ES2567161T3 - Procedimiento para el cultivo de células para la producción de sustancias - Google Patents
Procedimiento para el cultivo de células para la producción de sustancias Download PDFInfo
- Publication number
- ES2567161T3 ES2567161T3 ES10152393.4T ES10152393T ES2567161T3 ES 2567161 T3 ES2567161 T3 ES 2567161T3 ES 10152393 T ES10152393 T ES 10152393T ES 2567161 T3 ES2567161 T3 ES 2567161T3
- Authority
- ES
- Spain
- Prior art keywords
- glucose
- limitation
- dgl
- cells
- qglc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Procedimiento para el cultivo de células CHO para la producción de inmunoglobulinas o fragmentos de inmunoglobulinas, caracterizado por que se cultiva una célula CHO que produce sustancias con limitación de glucosa DGL, siendo el grado de limitación de glucosa DGL >= qGlc/qGlcmáx con qGlc >= tasa de consumo de glucosa específica observada en el momento y qGlcmáx >= máxima tasa de consumo de glucosa específica conocida para estas células CHO, mayor que el DGL que conduce al mantenimiento exclusivo, DGLmantenimiento, de la célula CHO y es >= 0,5, siendo el DGLmantenimiento >= qGlcmantenimiento/qGlcmáx, con qGlcmantenimiento >= tasa de consumo de glucosa específica observada durante el metabolismo de mantenimiento puro y qGlcmáx >= máxima tasa de consumo de glucosa específica conocida para estas células CHO, siendo la cantidad de glucosa suministrada no superior al 50 % de la que puede consumir como máximo el número de células esperado máximo sin limitación de glucosa, en el que se usa un medio que contiene glucosa que con respecto a otros componentes de sustancias nutritivas no está limitado antes del inicio de la limitación de glucosa, en el que el procedimiento se inicia como procedimiento discontinuo y se continúa como procedimiento continuo, en el que antes del inicio del procedimiento continuo se hace funcionar el cultivo como procedimiento discontinuo.
Description
- Tiempo de proceso
- cv D Alimentación de glucosa Glucosa Lactato MUC1-IgG2a gMUC1-IgG2a DGL
- 94
- 2,14E+06 0,035 23,89 8,08 19,48 15,49 0,30 1,00
- 120
- 3,70E+06 0,035 23,89 5,84 22,35 18,02 0,22 0,72
- 136,5
- 4,68E+06 0,035 23,89 4,30 22,02 19,95 0,17 0,62
- 163,5
- 7,02E+06 0,035 23,89 3,17 22,66 22,67 0,14 0,40
- 187,5
- 6,96E+06 0,035 23,89 1,79 20,77 22,44 0,11 0,44
- 215,5
- 8,85E+06 0,035 23,89 1,04 17,46 28,24 0,13 0,35
- 264,75
- 1,30E+07 0,035 23,89 - 8,45 67,03 0,22 0,24
- 287
- 1,54E+07 0,035 23,89 - 5,25 89,42 0,22 0,20
- 310
- 1,64E+07 0,035 23,89 - 2,77 113,28 0,25 0,19
- 331
- 2,27E+07 0,035 23,89 - 1,24 133,80 0,24 0,14
- 352,4
- 1,45E+07 0,035 23,89 - 0,82 152,87 0,29 0,21
- 376,3
- 1,42E+07 0,035 23,89 - 0,53 182,52 0,45 0,22
- 404,4
- 1,58E+07 0,035 23,89 - 0,44 218,51 0,51 0,20
- 428
- 1,78E+07 0,035 23,89 - 0,58 241,75 0,50 0,17
- 448,4
- 2,08E+07 0,035 23,89 - 0,55 305,39 0,55 0,15
- 473,63
- 1,35E+07 0,035 23,89 - 0,55 290,52 0,60 0,23
- 496,8
- 9,30E+06 0,035 23,89 - 0,51 274,94 0,85 0,33
- 521,82
- 1,53E+07 0,035 23,89 - 0,56 301,12 0,87 0,20
- Datos para la fermentación de MUC1-IgG2a
Tabla 2:
- Tiempo de proceso
- Número de células vivas D Alimentación de glucosa Glucosa Lactato MUC2-GFP-Cterm Producto q DGL
- h
- 1/ml 1/h mmol/l mmol/l mmol/l nM nmol h*E9
- 0,5
- 7,50E+04 0 0 21,37 3,12 0,00
- 106
- 1,80E+06 0 0 4,25 21,1 1,66 0,01 0,44
- 106,01
- 0,035 23,89 8,92
- 130
- 2,20E+06 0,035 23,89 9,36 7,71 0,14 0,66
- 154
- 2,90E+06 0,035 23,89 8,32 18,23 10,72 0,05 1,00
- 182,38
- 6,83E+06 0,035 23,89 5,58 19,28 14,08 0,17 0,53
- 212,9
- 1,19E+07 0,035 23,89 1,65 18,78 26,15 0,12 0,33
- 237,2
- 1,44E+07 0,035 23,89 0,54 13,84 38,37 0,11 0,26
- 254
- 1,48E+07 0,035 23,89 0,52 9,81 50,08 0,13 0,24
- 278
- 120E+07 0,035 23,89 - 5,19 65,63 0,20 0,35
- 302
- 1,40E+07 0,035 23,89 - 2,05 81,53 027 0,29
- 326
- 1,20E+07 0,035 23,89 - 0,7 88,03 0,30 0,34
- 349,9
- 2,16E+07 0,035 23,89 - 0,33 104,60 0,28 0,19
- 374
- 1,20E+07 0,035 23,89 - 0,26 104,03 0,28 0,34
- 0,035
- 23,89 - 84,47
- 0,035
- 23,89 - 75,16
- 446
- 1,10E+07 0,035 23,89 - 0,19 64,81 0,37
- 470
- 1,10E+07 0,035 23,89 - 0,53 52,36 0,37
- 494
- 1,40E+07 0,035 23,89 - 0,32 69,63 0,24 0,29
- 518
- 1,30E+07 0,035 23,89 - 79,34 0,26 0,32
- 0,035
- 23,89 - 93,94
- 0,035
- 23,89 - 0,35 104,57
7
Claims (1)
-
imagen1 imagen2
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10255508 | 2002-11-27 | ||
| DE10255508A DE10255508A1 (de) | 2002-11-27 | 2002-11-27 | Verfahren zur Kultivierung von Zellen zur Produktion von Substanzen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2567161T3 true ES2567161T3 (es) | 2016-04-20 |
Family
ID=32318751
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES03767406T Expired - Lifetime ES2340377T3 (es) | 2002-11-27 | 2003-11-07 | Procedimiento para el cultivo de celulas para la produccion de substancias. |
| ES10176622T Expired - Lifetime ES2749217T3 (es) | 2002-11-27 | 2003-11-07 | Procedimiento para cultivar células para la producción de sustancias |
| ES10152393.4T Expired - Lifetime ES2567161T3 (es) | 2002-11-27 | 2003-11-07 | Procedimiento para el cultivo de células para la producción de sustancias |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES03767406T Expired - Lifetime ES2340377T3 (es) | 2002-11-27 | 2003-11-07 | Procedimiento para el cultivo de celulas para la produccion de substancias. |
| ES10176622T Expired - Lifetime ES2749217T3 (es) | 2002-11-27 | 2003-11-07 | Procedimiento para cultivar células para la producción de sustancias |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US20060127975A1 (es) |
| EP (3) | EP1585810B1 (es) |
| JP (2) | JP4469283B2 (es) |
| AT (1) | ATE462013T1 (es) |
| CY (2) | CY1110267T1 (es) |
| DE (2) | DE10255508A1 (es) |
| DK (2) | DK1585810T3 (es) |
| ES (3) | ES2340377T3 (es) |
| HU (1) | HUE028862T2 (es) |
| PT (1) | PT1585810E (es) |
| SI (3) | SI2275530T1 (es) |
| WO (1) | WO2004048556A1 (es) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2322216A1 (en) | 1997-03-21 | 2011-05-18 | Chugai Seiyaku Kabushiki Kaisha | A preventive or therapeutic agent for sensitized T cell-mediated diseases comprising IL-6 antagonist as an active ingredient |
| UA80091C2 (en) | 2001-04-02 | 2007-08-27 | Chugai Pharmaceutical Co Ltd | Remedies for infant chronic arthritis-relating diseases and still's disease which contain an interleukin-6 (il-6) antagonist |
| GB2401040A (en) | 2003-04-28 | 2004-11-03 | Chugai Pharmaceutical Co Ltd | Method for treating interleukin-6 related diseases |
| EP1623019B2 (en) * | 2003-05-15 | 2017-01-25 | Wyeth LLC | Restricted glucose feed for animal cell culture |
| PE20091174A1 (es) | 2007-12-27 | 2009-08-03 | Chugai Pharmaceutical Co Ltd | Formulacion liquida con contenido de alta concentracion de anticuerpo |
| DE102008002210A1 (de) * | 2008-06-04 | 2009-12-10 | Evonik Degussa Gmbh | Verfahren zur fermentativen Herstellung von Erythropoietin |
| AU2016210748B2 (en) * | 2009-10-26 | 2018-03-01 | Chugai Seiyaku Kabushiki Kaisha | Method for the production of a glycosylated immunoglobulin |
| AU2015203309B2 (en) * | 2009-10-26 | 2016-05-05 | Chugai Seiyaku Kabushiki Kaisha | Method for the production of a glycosylated immunoglobulin |
| SI2493922T1 (sl) | 2009-10-26 | 2017-06-30 | F. Hoffmann-La Roche Ag | Postopek za proizvodnjo glikoziliranega imunoglobulina |
| TWI537386B (zh) * | 2009-12-22 | 2016-06-11 | Ｄｓｍ智慧財產有限公司 | 由發酵法獲得的６－胺己酸製備己內醯胺 |
| KR20190120439A (ko) | 2010-11-08 | 2019-10-23 | 제넨테크, 인크. | 피하 투여용 항―ｉｌ―6 수용체 항체 |
| BR112015032960B1 (pt) | 2013-07-04 | 2021-01-05 | F. Hoffmann-La Roche Ag | imunoensaio suprimido por interferência para detectar anticorpos anti-fármaco em amostras de soro |
| US10544395B2 (en) | 2014-03-19 | 2020-01-28 | Pfizer Inc. | Method of cell culture |
| JP7001585B2 (ja) | 2015-09-23 | 2022-02-04 | ファイザー・インク | 細胞および細胞培養の方法 |
| EP3891292A1 (en) | 2018-12-06 | 2021-10-13 | Pfizer Inc. | Cells with reduced inhibitor production and methods of use thereof |
| WO2025088516A1 (en) | 2023-10-26 | 2025-05-01 | Pfizer Inc. | Method of forming multi-specific antibodies from homodimer antibodies and pharmaceutical product including multi-specific antibodies thus obtained |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4657863A (en) * | 1982-07-02 | 1987-04-14 | Celanese Corporation | Stabilization of a mutant microorganism population |
| JPH066054B2 (ja) * | 1987-10-15 | 1994-01-26 | 帝人株式会社 | 動物細胞の培養方法 |
| JP3494300B2 (ja) * | 1990-04-03 | 2004-02-09 | ジェネンテック・インコーポレーテッド | Ｈｉｖに対する予防接種のための方法および組成物 |
| GB9022545D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| US5252216A (en) * | 1992-03-24 | 1993-10-12 | Smithkline Beecham Corporation | Protein purification |
| JPH08508875A (ja) * | 1992-05-01 | 1996-09-24 | 帝人株式会社 | 蛋白分泌細胞の流加回分培養法 |
| GB9506249D0 (en) * | 1995-03-27 | 1995-05-17 | Karobio Ab | Media for insect cell cultures |
| WO1998041611A1 (en) | 1997-03-20 | 1998-09-24 | Regents Of The University Of Minnesota | Process for the continuous culture of cells |
| US6338964B1 (en) * | 1999-05-07 | 2002-01-15 | Bayer Corporation | Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration |
-
2002
- 2002-11-27 DE DE10255508A patent/DE10255508A1/de not_active Withdrawn
-
2003
- 2003-11-07 JP JP2004554195A patent/JP4469283B2/ja not_active Expired - Lifetime
- 2003-11-07 DK DK03767406.6T patent/DK1585810T3/da active
- 2003-11-07 DE DE50312554T patent/DE50312554D1/de not_active Expired - Lifetime
- 2003-11-07 HU HUE10152393A patent/HUE028862T2/en unknown
- 2003-11-07 EP EP03767406A patent/EP1585810B1/de not_active Expired - Lifetime
- 2003-11-07 US US10/535,581 patent/US20060127975A1/en not_active Abandoned
- 2003-11-07 PT PT03767406T patent/PT1585810E/pt unknown
- 2003-11-07 DK DK10152393.4T patent/DK2226381T3/en active
- 2003-11-07 SI SI200332602T patent/SI2275530T1/sl unknown
- 2003-11-07 ES ES03767406T patent/ES2340377T3/es not_active Expired - Lifetime
- 2003-11-07 EP EP10152393.4A patent/EP2226381B1/de not_active Expired - Lifetime
- 2003-11-07 SI SI200332473A patent/SI2226381T1/sl unknown
- 2003-11-07 AT AT03767406T patent/ATE462013T1/de active
- 2003-11-07 ES ES10176622T patent/ES2749217T3/es not_active Expired - Lifetime
- 2003-11-07 EP EP10176622.8A patent/EP2275530B1/de not_active Expired - Lifetime
- 2003-11-07 SI SI200331791T patent/SI1585810T1/sl unknown
- 2003-11-07 ES ES10152393.4T patent/ES2567161T3/es not_active Expired - Lifetime
- 2003-11-07 WO PCT/DE2003/003693 patent/WO2004048556A1/de not_active Ceased
-
2010
- 2010-01-19 JP JP2010009094A patent/JP5221573B2/ja not_active Expired - Lifetime
- 2010-06-11 CY CY20101100518T patent/CY1110267T1/el unknown
-
2014
- 2014-01-21 US US14/160,336 patent/US20140329277A1/en not_active Abandoned
-
2016
- 2016-05-13 CY CY20161100413T patent/CY1117558T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2340377T3 (es) | 2010-06-02 |
| DE10255508A1 (de) | 2004-06-17 |
| EP2226381A1 (de) | 2010-09-08 |
| DK2226381T3 (en) | 2016-04-11 |
| SI1585810T1 (sl) | 2010-06-30 |
| JP2006507821A (ja) | 2006-03-09 |
| JP4469283B2 (ja) | 2010-05-26 |
| ES2749217T3 (es) | 2020-03-19 |
| JP2010110331A (ja) | 2010-05-20 |
| ATE462013T1 (de) | 2010-04-15 |
| JP5221573B2 (ja) | 2013-06-26 |
| SI2226381T1 (sl) | 2016-05-31 |
| HUE028862T2 (en) | 2017-01-30 |
| EP1585810B1 (de) | 2010-03-24 |
| EP1585810A1 (de) | 2005-10-19 |
| EP2275530B1 (de) | 2019-08-07 |
| CY1117558T1 (el) | 2017-04-26 |
| EP2275530A2 (de) | 2011-01-19 |
| PT1585810E (pt) | 2010-05-05 |
| US20140329277A1 (en) | 2014-11-06 |
| DK1585810T3 (da) | 2010-06-07 |
| SI2275530T1 (sl) | 2019-11-29 |
| EP2226381B1 (de) | 2016-03-09 |
| DE50312554D1 (de) | 2010-05-06 |
| US20060127975A1 (en) | 2006-06-15 |
| EP2275530A3 (de) | 2012-05-23 |
| WO2004048556A1 (de) | 2004-06-10 |
| CY1110267T1 (el) | 2015-01-14 |
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