ES2570828T3 - Método de análisis de metilación - Google Patents

Método de análisis de metilación

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Publication number
ES2570828T3
ES2570828T3 ES12164321T ES12164321T ES2570828T3 ES 2570828 T3 ES2570828 T3 ES 2570828T3 ES 12164321 T ES12164321 T ES 12164321T ES 12164321 T ES12164321 T ES 12164321T ES 2570828 T3 ES2570828 T3 ES 2570828T3
Authority
ES
Spain
Prior art keywords
terminal
oligonucleotide
nucleotides
seq
variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES12164321T
Other languages
English (en)
Inventor
Theo Devos
Cathy Lofton-Day
Andrew Sledziewski
Fabian Model
Michael Krouse
Mathias Schuster
Jürgen Distler
Reimo Tetzner
Jesse Ho
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Application granted granted Critical
Publication of ES2570828T3 publication Critical patent/ES2570828T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Un método para análisis de metilación, comprende a) tratar ADN genómico con uno o más reactivos para convertir bases de citosina no metiladas a sulfonato de uracilo o a otra base que tenga un comportamiento de unión diferente de citosina, mientras que la citosina metilada permanece sin cambio; b) amplificar el ADN tratado por medio de i) un oligonucleótido que comprende o que consiste de una secuencia definida por la SEQ ID NO: 5 o una variante de la misma con supresión de 5'-terminal y/o 3'-terminal de 1, 2, 3, 4 o 5 nucleótidos; y ii) un oligonucleótido que comprende o que consiste de una secuencia que se define por la SEQ ID NO: 44 o una variante de la misma con supresión de 5'-terminal y/o 3'-terminal de 1, 2, 3, 4 o 5 nucleótidos; en donde dichos oligonucleótidos son adecuados para uso como cebadores; c) deducir la presencia o ausencia de metilación de los dinucleótidos CpG amplificados en la etapa b) a partir de los resultados de la etapa b).
ES12164321T 2007-06-08 2008-06-06 Método de análisis de metilación Active ES2570828T3 (es)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP07109907 2007-06-08
EP07110409 2007-06-15
EP07113516 2007-07-31
EP07114659 2007-08-21
EP07114863 2007-08-23
EP08150552 2008-01-23
EP08151442 2008-02-14

Publications (1)

Publication Number Publication Date
ES2570828T3 true ES2570828T3 (es) 2016-05-20

Family

ID=40094240

Family Applications (2)

Application Number Title Priority Date Filing Date
ES12164321T Active ES2570828T3 (es) 2007-06-08 2008-06-06 Método de análisis de metilación
ES16163841T Active ES2769841T3 (es) 2007-06-08 2008-06-06 Método de análisis de metilación

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES16163841T Active ES2769841T3 (es) 2007-06-08 2008-06-06 Método de análisis de metilación

Country Status (11)

Country Link
US (1) US8623599B2 (es)
EP (3) EP2152902A2 (es)
CY (1) CY1118259T1 (es)
DK (2) DK2479289T3 (es)
ES (2) ES2570828T3 (es)
HR (2) HRP20160425T1 (es)
HU (1) HUE028727T2 (es)
LT (1) LT3101141T (es)
PL (1) PL2479289T3 (es)
SI (2) SI2479289T1 (es)
WO (1) WO2008149237A2 (es)

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JP6153866B2 (ja) 2010-05-25 2017-06-28 キアゲン ガイサーズバーグ アイエヌシー． 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ
US9725754B2 (en) * 2010-07-29 2017-08-08 Sean F. Boyle Generic sample preparation
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
CN103597095B (zh) 2011-02-24 2016-08-17 奇亚根盖瑟斯堡股份有限公司 用于检测hpv核酸的材料和方法
CA2863215C (en) 2012-01-30 2021-05-04 Exact Sciences Corporation Modification of dna on magnetic beads
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EP2746403A1 (de) 2012-12-21 2014-06-25 Life Science Inkubator GmbH & Co. KG Verfahren zur Bestimmung des DNA-Methylierungsgrades
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EP3132055A2 (en) 2014-04-14 2017-02-22 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. A method and kit for determining the tissue or cell origin of dna
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WO2016097319A1 (en) * 2014-12-19 2016-06-23 Epigenomics Ag METHODS FOR DETECTING CpG METHYLATION AND FOR DIAGNOSING CANCER
CN107532124B (zh) 2015-03-27 2022-08-09 精密科学公司 检测食管疾病
CN108138235B (zh) 2015-08-31 2022-04-15 梅约医药教育及研究基金会 检测胃肿瘤
AU2016343937B2 (en) 2015-10-30 2023-01-19 Exact Sciences Corporation Multiplex amplification detection assay and isolation and detection of DNA from plasma
SG10201912967YA (en) * 2015-11-27 2020-02-27 Agency Science Tech & Res Determination of nucleic acid methylation
CN105506133A (zh) * 2016-01-15 2016-04-20 武汉艾米森生命科技有限公司 用于检测sept9基因甲基化的组合物、试剂盒及方法
CN116064796A (zh) 2016-05-05 2023-05-05 精密科学公司 通过分析甲基化dna来检测肺肿瘤
KR102892245B1 (ko) 2017-01-27 2025-11-27 이그젝트 싸이언스 디블롭먼트 컴패니, 엘엘씨 메틸화된 dna 분석에 의한 결장 신조직형성의 검출
CN107164524A (zh) * 2017-06-27 2017-09-15 深圳市优圣康生物科技有限公司 用于基因甲基化检测的引物和探针、采样方法、试剂盒
US20200165671A1 (en) * 2017-07-13 2020-05-28 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Detecting tissue-specific dna
US11466323B2 (en) 2017-07-13 2022-10-11 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Dual-probe digital droplet PCR strategy for specific detection of tissue-specific circulating DNA molecules
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
CN108048570A (zh) * 2017-12-29 2018-05-18 韩林志 用于结直肠癌相关基因Septin9甲基化检测的引物对、试剂盒及方法
CN110283913A (zh) * 2019-07-21 2019-09-27 广州奥百阕谱生物科技有限公司 Rassf1a基因甲基化状态检测试剂盒及其应用
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Also Published As

Publication number Publication date
US20110009277A1 (en) 2011-01-13
WO2008149237A2 (en) 2008-12-11
EP3101141A3 (en) 2017-01-11
DK3101141T3 (da) 2020-02-03
ES2769841T3 (es) 2020-06-29
HUE028727T2 (en) 2017-01-30
EP3101141A2 (en) 2016-12-07
WO2008149237A3 (en) 2009-06-04
EP3101141B1 (en) 2019-10-16
EP2152902A2 (en) 2010-02-17
PL2479289T3 (pl) 2016-10-31
SI2479289T1 (sl) 2016-10-28
DK2479289T3 (en) 2016-05-02
HRP20160425T1 (hr) 2016-08-12
HRP20200059T1 (hr) 2020-04-03
EP2479289B1 (en) 2016-04-06
HK1172656A1 (en) 2013-04-26
SI3101141T1 (sl) 2020-03-31
EP2479289A2 (en) 2012-07-25
EP2479289A3 (en) 2013-06-19
US8623599B2 (en) 2014-01-07
LT3101141T (lt) 2020-03-10
CY1118259T1 (el) 2017-06-28

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