ES2600955T3 - Adenovirus oncolíticos para el tratamiento del cáncer - Google Patents

Adenovirus oncolíticos para el tratamiento del cáncer Download PDF

Info

Publication number
ES2600955T3
ES2600955T3 ES10772050.0T ES10772050T ES2600955T3 ES 2600955 T3 ES2600955 T3 ES 2600955T3 ES 10772050 T ES10772050 T ES 10772050T ES 2600955 T3 ES2600955 T3 ES 2600955T3
Authority
ES
Spain
Prior art keywords
adenovirus
oncolytic adenovirus
oncolytic
tumor
cancer treatment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES10772050.0T
Other languages
English (en)
Other versions
ES2600955T5 (en
Inventor
Sònia GUEDAN CARRIÓ
Manel Maria Cascallo Piqueras
Ramón ALEMANY BONASTRE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fundacio Privada Inst D'investigacio Biomedica
Fundacio Privada Institut D'investigacio Biomedica
Institut Català d'Oncologia
Original Assignee
Fundacio Privada Inst D'investigacio Biomedica
Fundacio Privada Institut D'investigacio Biomedica
Institut Català d'Oncologia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43050014&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2600955(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Fundacio Privada Inst D'investigacio Biomedica, Fundacio Privada Institut D'investigacio Biomedica, Institut Català d'Oncologia filed Critical Fundacio Privada Inst D'investigacio Biomedica
Application granted granted Critical
Publication of ES2600955T3 publication Critical patent/ES2600955T3/es
Publication of ES2600955T5 publication Critical patent/ES2600955T5/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/76—Viruses; Subviral particles; Bacteriophages
    • A61K35/761—Adenovirus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/861—Adenoviral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2474—Hyaluronoglucosaminidase (3.2.1.35), i.e. hyaluronidase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10321—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10332—Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10371—Demonstrated in vivo effect

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La invención se refiere a un adenovirus oncolítico que comprende una secuencia que codifica una enzima hialuronidasa insertada en su genoma. Este adenovirus se distribuye más eficientemente por la masa tumoral y por consiguiente se aumenta el efecto oncolítico. Inyectando el adenovirus oncolítico de la invención endovenosamente se obtienen regresiones del volumen tumoral. Por lo tanto el adenovirus oncolítico de la presente invención es útil para el tratamiento del cáncer o de un estado pre-maligno del mismo.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
adenovírica a modificar, y después realizar una recombinación homóloga en bacterias con un plásmido que contiene el resto del genoma vírico.
El adenovirus que contiene el gen de la hialuronidasa objeto de la presente invención se propaga y amplifica en
5 líneas celulares normalmente utilizadas en el campo de la terapia génica y viroterapia, tales como las líneas HEK-293 y A549. El método preferente de propagación es por infección de una línea celular permisiva a la replicación del adenovirus. La línea de adenocarcinoma pulmonar A549 es un ejemplo de una línea con tales características. La propagación se realiza por ejemplo del siguiente modo: las células A549 se siembran en placas de cultivo celular de plástico y se infectan utilizando 100 partículas víricas por célula. Dos días después el efecto
10 citopático que refleja la producción de virus se observa como un agrupamiento y redondamiento de las células. Las células se recogen y se almacenan en tubos. Después de una centrifugación a 1000 g durante 5 minutos, el sedimento celular se congela y descongela tres veces para romper las células. El extracto celular resultante se centrifuga a 1000 g durante 5 minutos y el sobrenadante con virus se carga en un gradiente de cloruro de cesio y se centrifuga durante 1 hora a 35000 g. La banda de virus obtenida del gradiente se carga de nuevo en otro
15 gradiente de cloruro de cesio y se centrifuga otra vez durante 16 horas a 35000 g. La banda de virus se recoge y se dializa frente a PBS-glicerol al 10%. El virus dializado se alícuota y mantiene a -80 ºC. La cuantificación del número de partículas víricas y de unidades formadoras de placa se realiza siguiendo protocolos convencionales. La solución salina tamponada con fosfato (PBS) con glicerol al 5% es una formulación convencional para el almacenamiento de adenovirus. No obstante, se han descrito nuevas formulaciones que mejoran la estabilidad del
20 virus. Los métodos de purificación del adenovirus que contiene el gen de la hialuronidasa para su utilización en el tratamiento del cáncer, son los mismos que los descritos para otros adenovirus y vectores adenovíricos utilizados en viroterapia y terapia génica del cáncer.
El adenovirus oncolítico de la presente invención puede administrarse a un mamífero, preferentemente un humano.
25 La intención de la administración del adenovirus oncolítico es terapéutica, incluyendo, pero sin limitación, melanoma, cáncer de páncreas, cáncer de colon y cáncer de pulmón. Además, se contempla la administración del adenovirus oncolítico en una fase pre-maligno de un tumor.
Se entiende que el adenovirus oncolítico se administra en una forma farmacéuticamente aceptable. Los expertos
30 en la materia pueden cerciorarse de la dosis apropiada utilizando procedimientos convencionales. Se entiende que la dosis debe ser una cantidad eficaz del adenovirus oncolítico para que produzca una reducción del tumor en el paciente tratado. El virus se puede administrar de forma directa virus al tumor, en la cavidad donde se emplaza el tumor, en la vasculatura del tumor, alrededor del tumor o por la inyección endovenosa sistémica en el paciente. Preferentemente, la administración es sistémica.
35 Los protocolos para utilizar los virus descritos en la presente invención para el tratamiento del cáncer son los mismos procedimientos utilizados en los campos de la viroterapia con adenovirus y terapia génica con adenovirus. Existe una amplia experiencia en el uso de adenovirus no oncolíticos y oncolíticos en el campo de la terapia génica. Existen numerosas publicaciones que describen el tratamiento de células tumorales en cultivo, en modelos
40 animales y en ensayos clínicos con pacientes. Para el tratamiento de células en cultivos in vitro el adenovirus purificado mediante cualquiera de las formulaciones descritas anteriormente, se añade al medio de cultivo para obtener la infección de las células tumorales. Para tratar tumores en modelos animales o en pacientes humanos, el adenovirus se puede administrar loco-regionalmente mediante inyección en el tumor o en una cavidad corporal donde se localiza el tumor, o bien por vía sistémica mediante inyección en la circulación sanguínea.
45 El adenovirus oncolítico de la invención puede administrarse solo o en una composición con transportadores o excipientes farmacéuticamente aceptables. El experto en la materia adaptará la composición de acuerdo con el modo particular de administración. Las composiciones pueden comprender el adenovirus oncolítico como el único agente contra el tumor, o en combinación con otro agente terapéutico tal como un fármaco quimioterapéutico o un
50 vector con un gen terapéutico insertado. También puede combinarse la terapia con el adenovirus oncolítico con radioterapia.
A menos que se defina de otro modo, todos los términos técnicos y científicos utilizados en el presente documento tienen el mismo significado a los que entiende comúnmente una persona experta en la materia. En la práctica de la 55 presente invención pueden utilizarse métodos y materiales similares o equivalentes a los descritos en el presente documento. A lo largo de la descripción y las reivindicaciones la palabra "comprende" y sus variantes no pretenden excluir otras características técnicas, aditivos, componentes o etapas. Los objetivos, ventajas y características adicionales de la invención, serán evidentes para los expertos en la materia después de examinar la descripción o pueden aprenderse mediante la práctica de la invención. Las siguientes realizaciones particulares y los dibujos se
60 proporcionan a modo de ilustración, y no se pretende que sean limitativos de la presente invención.
Descripción de los dibujos
La FIG. 1 (a) muestra la estructura de adenovirus oncolíticos caracterizados por contener y expresar el gen de la
7
imagen6
imagen7
imagen8
imagen9
imagen10
imagen11
imagen12

Claims (1)

  1. imagen1
    imagen2
ES10772050T 2009-05-06 2010-05-05 Oncolytic adenoviruses for treating cancer Active ES2600955T5 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ES200901201 2009-05-06
ES200901201A ES2385251B1 (es) 2009-05-06 2009-05-06 Adenovirus oncolíticos para el tratamiento del cáncer.
PCT/ES2010/000196 WO2010128182A1 (es) 2009-05-06 2010-05-05 Adenovirus oncolíticos para el tratamiento del cáncer

Publications (2)

Publication Number Publication Date
ES2600955T3 true ES2600955T3 (es) 2017-02-13
ES2600955T5 ES2600955T5 (en) 2025-10-02

Family

ID=43050014

Family Applications (2)

Application Number Title Priority Date Filing Date
ES200901201A Expired - Fee Related ES2385251B1 (es) 2009-05-06 2009-05-06 Adenovirus oncolíticos para el tratamiento del cáncer.
ES10772050T Active ES2600955T5 (en) 2009-05-06 2010-05-05 Oncolytic adenoviruses for treating cancer

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES200901201A Expired - Fee Related ES2385251B1 (es) 2009-05-06 2009-05-06 Adenovirus oncolíticos para el tratamiento del cáncer.

Country Status (15)

Country Link
US (3) US10316065B2 (es)
EP (1) EP2428229B2 (es)
JP (1) JP2012525833A (es)
KR (1) KR101878274B1 (es)
CN (2) CN107252438A (es)
AU (1) AU2010244348B2 (es)
BR (1) BRPI1011445B8 (es)
CA (1) CA2761183C (es)
ES (2) ES2385251B1 (es)
FI (1) FI2428229T4 (es)
HK (1) HK1244679A1 (es)
MX (1) MX2011011818A (es)
PL (1) PL2428229T5 (es)
RU (1) RU2536931C2 (es)
WO (1) WO2010128182A1 (es)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2330213A1 (en) 2003-03-05 2011-06-08 Halozyme, Inc. Soluble hyaluronidase glycoprotein (sHASEGP), process for preparing the same, uses and pharmaceutical compositions comprising thereof
ES2385251B1 (es) * 2009-05-06 2013-05-06 Fundació Privada Institut D'investigació Biomèdica De Bellvitge Adenovirus oncolíticos para el tratamiento del cáncer.
SG11201507393TA (en) 2013-03-14 2015-10-29 Salk Inst For Biological Studi Oncolytic adenovirus compositions
CN103405480B (zh) * 2013-05-03 2015-11-25 浙江理工大学 10-羟基喜树碱-丁二酸与腺病毒结合物及制法和用途
CN103614416B (zh) * 2013-09-30 2016-09-07 中国人民解放军第二军医大学东方肝胆外科医院 一种携带人穿膜肽p53与GM-CSF基因的重组溶瘤腺病毒及其用途
KR102272932B1 (ko) * 2013-10-25 2021-07-05 싸이오서스 테라퓨틱스 엘티디. 이종 유전자로 무장된 종양살상 아데노바이러스
EP2940128A1 (en) 2014-04-30 2015-11-04 Institut d'Investigació Biomèdica de Bellvitge (IDIBELL) Adenovirus comprising an albumin-binding moiety
EP3186281B1 (en) 2014-08-28 2019-04-10 Halozyme, Inc. Combination therapy with a hyaluronan-degrading enzyme and an immune checkpoint inhibitor
ES2899913T3 (es) 2014-09-24 2022-03-15 Salk Inst For Biological Studi Virus tumorales oncolíticos y métodos de uso
AU2015332533B2 (en) 2014-10-14 2018-08-30 Halozyme, Inc. Compositions of adenosine deaminase-2 (ADA2), variants thereof and methods of using same
GB201505860D0 (en) 2015-04-07 2015-05-20 Agalimmune Ltd Therapeutic compositions and methods of use for treating cancer
DK3288573T3 (da) 2015-04-30 2020-03-16 Psioxus Therapeutics Ltd Onkolytisk adenovirus, der koder for et b7-protein
CN107849111B (zh) * 2015-05-04 2022-02-22 Vcn生物科学有限公司 在免疫显性腺病毒抗原表位中具有突变的溶瘤腺病毒及其在癌症治疗中的用途
CN104946601A (zh) * 2015-05-28 2015-09-30 金宁一 重组溶瘤腺病毒及其应用
US11155622B2 (en) 2015-12-17 2021-10-26 Psioxus Therapeutics Limited Virus encoding an anti-TCR-complex antibody or fragment
EP3390645B1 (en) 2016-02-23 2022-09-14 Salk Institute for Biological Studies Exogenous gene expression in therapeutic adenovirus for minimal impact on viral kinetics
WO2017147265A1 (en) 2016-02-23 2017-08-31 Salk Institute For Biological Studies High throughput assay for measuring adenovirus replication kinetics
KR102323872B1 (ko) * 2016-04-22 2021-11-08 임비라 컴퍼니 리미티드 암치료용 종양 용해성 단순 헤르페스 바이러스(oHSV) 절대 벡터 및 그 구조체의 구조
KR20240011868A (ko) * 2016-04-29 2024-01-26 이노비오 파마수티컬즈, 인크. 제제의 전달을 향상시키기 위한 콘드로이티나제 및/또는 히알루로니다제의 생체내 용도
WO2017201635A1 (zh) * 2016-05-23 2017-11-30 蔡胜和 透明质酸酶的细胞表达及其在实体瘤细胞治疗中的应用
EP3478321A4 (en) * 2016-06-30 2020-04-22 Oncorus, Inc. PSEUDOTYPIZED ONCOLYTIC VIRAL ADMINISTRATION OF THERAPEUTIC POLYPEPTIDES
KR102491752B1 (ko) 2016-08-29 2023-01-26 싸이오서스 테라퓨틱스 엘티디. 이중특이성 T 세포 세포 관여자(BiTE)가 보강된 아데노바이러스
GB201713765D0 (en) 2017-08-28 2017-10-11 Psioxus Therapeutics Ltd Modified adenovirus
CN109982709A (zh) * 2016-11-17 2019-07-05 Vcn生物科学有限公司 病毒载体在治疗视网膜母细胞瘤中的用途
AU2017375633C1 (en) 2016-12-12 2023-04-27 Salk Institute For Biological Studies Tumor-targeting synthetic adenoviruses and uses thereof
WO2019089755A1 (en) 2017-10-31 2019-05-09 Western Oncolytics Ltd. Platform oncolytic vector for systemic delivery
EP3708657A4 (en) 2017-11-08 2021-08-25 Kagoshima University ONCOLYTIC VIRUS (ONCOLYTIC IMMUNOTHERAPY) THAT IS CAPABLE OF TREATING METASTATIC CANCER ALSO WITH ENSURED SAFETY, WITH EXPRESSION CONTROL SYSTEM THAT BUILDS AN OPTIMAL EXPRESSION LEVEL
WO2019102268A1 (en) 2017-11-22 2019-05-31 Mesoblast International Sarl Cellular compositions and methods of treatment i
CN109913422A (zh) * 2017-12-13 2019-06-21 苏州康聚生物科技有限公司 一种包含肿瘤抗原识别受体的免疫细胞及其应用
WO2019133847A1 (en) 2017-12-29 2019-07-04 Oncorus, Inc. Oncolytic viral delivery of therapeutic polypeptides
AU2019208892B2 (en) * 2018-01-19 2022-02-03 Kolon Life Science, Inc. Recombinant vaccinia virus and pharmaceutical composition comprising same
WO2019174610A1 (zh) * 2018-03-14 2019-09-19 蔡立刚 一种溶瘤病毒、合成dna序列及其应用
EP3773649A4 (en) 2018-03-28 2022-02-23 EpicentRx, Inc. PERSONALIZED CANCER VACCINE
BR112020019942A2 (pt) 2018-04-09 2021-01-26 Salk Institute For Biological Studies composições de adenovírus oncolítico com propriedades de replicação aprimoradas
MX2020009824A (es) 2018-07-25 2021-01-15 Alteogen Inc Nuevas variantes de hialuronidasa y composicion farmaceutica que comprende la misma.
US20210340279A1 (en) 2018-08-31 2021-11-04 Yale University Compositions and methods of using cell-penetrating antibodies in combination with immune checkpoint modulators
CN112203642B (zh) 2019-03-25 2025-03-07 阿特根公司 用于皮下注射的包含人透明质酸酶ph20变体和药物的药物组合物
KR20220038485A (ko) 2019-08-05 2022-03-28 메조블라스트 인터내셔널 에스에이알엘 바이러스 벡터를 포함하는 세포 조성물 및 치료 방법
TW202615652A (zh) 2020-01-23 2026-04-16 南韓商阿特根公司 新穎的具有改善的穩定性的玻尿酸酶變異體及含有其的醫藥組合物
CN111909962A (zh) * 2020-07-23 2020-11-10 药鼎(北京)国际细胞医学技术有限公司 一种治疗肝癌的病毒构建体及其用途和构建方法
WO2022031093A1 (ko) 2020-08-07 2022-02-10 (주)알테오젠 재조합 히알루로니다제의 생산 방법
CN115996735A (zh) 2020-08-10 2023-04-21 迈索布拉斯特国际有限公司 细胞组合物和治疗方法
RU2753742C1 (ru) * 2020-10-16 2021-08-24 Федеральное государственное автономное образовательное учреждение высшего образования "Новосибирский национальный исследовательский государственный университет" (Новосибирский государственный университет, НГУ) Рекомбинантный штамм Ad6-hTERT-GMCSF, содержащий встройку промотора теломеразы человека hTERT, а также гена гранулоцитарно-макрофагального колониестимулирующего фактора человека, обладающий избирательной цитолитической активностью против теломераза-положительных опухолевых клеток и экспрессирующий активный человеческий гранулоцитарно-макрофагальный колониестимулирующий фактор
JP2023552289A (ja) 2020-11-19 2023-12-15 カリヴィル イムノセラピューティクス， インコーポレイテッド 腫瘍微小環境リモデリングによる腫瘍溶解性免疫療法
EP4329786A4 (en) 2021-04-30 2024-12-11 KaliVir Immunotherapeutics, Inc. Oncolytic viruses for modified mhc expression
CN114703186B (zh) * 2022-03-31 2023-08-29 四川大学 肿瘤特异性启动子及其应用
CN117379389B (zh) * 2022-07-12 2026-03-06 四川大学 血小板-溶瘤病毒复合物、其制备方法及其应用
WO2024062372A1 (en) * 2022-09-19 2024-03-28 Theriva Biologics, S.L. Combination therapies with oncolytic adenovirus and topoisomerase i inhibitors or prodrugs thereof
JP2025536260A (ja) 2022-10-11 2025-11-05 イェール ユニバーシティー 細胞透過性抗体の組成物および使用方法
KR20260077739A (ko) * 2023-09-20 2026-05-28 테리바 바이오로직스 에스.엘. 암의 치료에 사용하기 위한 종양용해성 아데노바이러스와 토포아이소머라제 i 저해제

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL97312A (en) 1990-02-23 1999-01-26 Lilly Co Eli A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient
US5721348A (en) 1990-12-14 1998-02-24 University Of Connecticut DNA encoding PH-20 proteins
EP0872552A1 (en) 1997-04-15 1998-10-21 Leadd B.V. A gene delivery vehicle expressing the apoptosis-inducing proteins VP2 and/or Apoptin
US5516631A (en) 1992-10-13 1996-05-14 La Jolla Cancer Research Foundation Method of inhibiting replication of hyperproliferative cells
GB9223084D0 (en) 1992-11-04 1992-12-16 Imp Cancer Res Tech Compounds to target cells
US5846945A (en) 1993-02-16 1998-12-08 Onyx Pharmaceuticals, Inc. Cytopathic viruses for therapy and prophylaxis of neoplasia
DK0689447T3 (da) 1993-02-16 1999-10-18 Onyx Pharma Inc Cytopatiske vira til behandling af og profylakse for neoplasia
GB9415167D0 (en) 1994-07-27 1994-09-14 Springer Caroline J Improvements relating to cancer therapy
US5998205A (en) 1994-11-28 1999-12-07 Genetic Therapy, Inc. Vectors for tissue-specific replication
US6326356B1 (en) 1996-10-18 2001-12-04 Board Of Regents, The University Of Texas System Suppression of neu overexpression using a mini-E1A gene
US20040048821A1 (en) 1997-06-27 2004-03-11 Cold Spring Harbor Laboratory Enhancement of drug cytotoxicity in tumor cells containing mutant Rb gene
CN1304316A (zh) 1998-02-06 2001-07-18 亚拉巴马大学伯明翰分校研究基金会 在纤维结的hi环中含有异源肽表位的腺病毒载体
US6900049B2 (en) 1998-09-10 2005-05-31 Cell Genesys, Inc. Adenovirus vectors containing cell status-specific response elements and methods of use thereof
CZ301506B6 (cs) 1998-10-15 2010-03-31 Canji, Inc. Selektivne se replikující rekombinantní virový vektor a zpusob jeho prípravy, farmaceutická formulace, zpusob usmrcení bunky s defektní dráhou, transformovaná bunka a promotor reagující na dráhu p53 a TGF-ß
US20020037274A1 (en) 1998-10-26 2002-03-28 Angelica Williams Single agent method for killing tumor and tumor associated endothelial cells using adenoviral mutants
WO2000029599A1 (en) 1998-11-18 2000-05-25 Canji, Inc. Viral vectors with late transgene expression
US6495130B1 (en) 1998-12-30 2002-12-17 Calydon, Inc. Target cell-specific adenoviral vectors containing E3 and methods of use thereof
EP1180932B1 (en) 1999-05-12 2012-01-11 The Uab Research Foundation Infectivity-enhanced conditionally-replicative adenovirus and uses thereof
US7078030B2 (en) 1999-11-15 2006-07-18 Onyx Pharmaceuticals, Inc Oncolytic adenovirus
WO2001036650A2 (en) 1999-11-15 2001-05-25 Onyx Pharmaceuticals, Inc. An oncolytic adenovirus
US7306902B2 (en) 2002-06-28 2007-12-11 Oncolyties Biotech Inc. Oncolytic viruses as phenotyping agents for neoplasms
US20020164715A1 (en) 2000-07-10 2002-11-07 Lin Ji Chromosome 3p21.3 genes are tumor suppressors
EP1203819A3 (en) 2000-10-06 2002-08-07 Transgene S.A. Anti-inflammatory vectors
IL152420A0 (en) 2001-02-23 2003-05-29 Novartis Ag Novel oncolytic adenoviral vectors
CA2439185A1 (en) 2001-02-23 2002-09-06 Novartis Ag Vector constructs
NZ527687A (en) 2001-05-11 2005-10-28 Wellstat Biolog Corp Formerly Preferential binding of an oncolytic virus such as Newcastle Disease Virus (NDV) to leukocytes compared with erythrocytes used as a therapy for cancer
EP1516055A4 (en) 2002-01-24 2007-08-08 Scripps Research Inst FIBER OPERATING MODIFICATIONS FOR EFFICIENT TARGETS
AU2003241268A1 (en) 2002-02-05 2003-09-09 Cornell Research Foundation, Inc. Adenoviral vector with replication-dependent transgene expression
US20060147420A1 (en) 2004-03-10 2006-07-06 Juan Fueyo Oncolytic adenovirus armed with therapeutic genes
AU2003220115A1 (en) 2002-05-20 2003-12-12 Board Of Regents, The University Of Texas System Methods and compositions for delivering enzymes and nucleic acid molecules to brain, bone, and other tissues
EP2330213A1 (en) 2003-03-05 2011-06-08 Halozyme, Inc. Soluble hyaluronidase glycoprotein (sHASEGP), process for preparing the same, uses and pharmaceutical compositions comprising thereof
US20060104968A1 (en) 2003-03-05 2006-05-18 Halozyme, Inc. Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminogly ycanases
CA2519680A1 (en) * 2003-03-28 2004-11-18 The Scripps Research Institute Adenovirus particles with enhanced infectivity of dendritic cells and particles with decreased infectivity of hepatocytes
WO2005018332A1 (en) * 2003-08-13 2005-03-03 The General Hospital Corporation Modified microorganisms for anti-cancer therapy
US20070036721A1 (en) * 2003-09-23 2007-02-15 Uab Resarch Foundation Methods and compositions for in vivo inflammation monitoring
US20060292682A1 (en) * 2004-07-22 2006-12-28 Hawkins Lynda K Addition of transgenes into adenoviral vectors
US8658778B2 (en) 2005-03-09 2014-02-25 Board Of Regents, The University Of Texas System hTMC promoter and vectors for the tumor-selective and high-efficient expression of cancer therapeutic genes
WO2007059473A2 (en) * 2005-11-12 2007-05-24 Introgen Therapeutics, Inc. Methods for the production and purification of adenoviral vectors
ATE516370T1 (de) 2005-12-22 2011-07-15 Sloan Kettering Inst Cancer Verfahren zur erfassung von krebszellen unter verwendung eines virus
CN101970650B (zh) * 2008-03-06 2017-07-25 哈洛齐梅公司 可溶性透明质酸酶的大规模生产
ES2385251B1 (es) * 2009-05-06 2013-05-06 Fundació Privada Institut D'investigació Biomèdica De Bellvitge Adenovirus oncolíticos para el tratamiento del cáncer.
BR112020015488A2 (pt) * 2018-02-08 2020-12-08 Sangamo Therapeutics, Inc. Nucleases alvo-específicas modificadas

Also Published As

Publication number Publication date
HK1167818A1 (en) 2012-12-14
CA2761183A1 (en) 2010-11-11
EP2428229B2 (en) 2025-05-21
PL2428229T3 (pl) 2017-02-28
US20190345204A1 (en) 2019-11-14
MX2011011818A (es) 2012-06-19
BRPI1011445B1 (pt) 2022-10-04
BRPI1011445B8 (pt) 2022-10-25
US20250011373A1 (en) 2025-01-09
WO2010128182A1 (es) 2010-11-11
ES2385251A1 (es) 2012-07-20
KR20120049185A (ko) 2012-05-16
AU2010244348A1 (en) 2011-12-01
KR101878274B1 (ko) 2018-07-13
BRPI1011445A2 (pt) 2020-09-15
US20120148535A1 (en) 2012-06-14
RU2536931C2 (ru) 2014-12-27
RU2011149458A (ru) 2013-06-27
US10316065B2 (en) 2019-06-11
ES2600955T5 (en) 2025-10-02
PL2428229T5 (pl) 2025-08-18
ES2385251B1 (es) 2013-05-06
CN107252438A (zh) 2017-10-17
CA2761183C (en) 2019-03-26
EP2428229A1 (en) 2012-03-14
CN102548584A (zh) 2012-07-04
HK1244679A1 (zh) 2018-08-17
FI2428229T4 (fi) 2025-07-29
AU2010244348B2 (en) 2016-08-11
EP2428229B1 (en) 2016-08-03
JP2012525833A (ja) 2012-10-25
US12129280B2 (en) 2024-10-29

Similar Documents

Publication Publication Date Title
Zhang et al. S-RBD-modified and miR-486-5p-engineered exosomes derived from mesenchymal stem cells suppress ferroptosis and alleviate radiation-induced lung injury and long-term pulmonary fibrosis
ES2600955T5 (en) Oncolytic adenoviruses for treating cancer
JP6188728B2 (ja) 核酸送達の区画化方法ならびにその組成物および使用
ES2466415T3 (es) Construcción de recombinante de adenovirus oncolítico que expresa específicamente el factor inmunomodulador GM-CSF en células tumorales y usos del mismo
JP2016531670A (ja) 低侵襲処置用クランプデバイスおよびその使用
US10744172B2 (en) Tropism modified cancer terminator virus (Ad.5/3 CTV;Ad.5/3-CTV-M7)
Wirtz et al. Efficient gene delivery to the inflamed colon by local administration of recombinant adenoviruses with normal or modified fibre structure
ES2231281T3 (es) Vectores competentes de replicacion anticancerosos.
Nisar et al. An extensive review on preclinical and clinical trials of oncolytic viruses therapy for pancreatic cancer
Oyama et al. Intravesical and intravenous therapy of human bladder cancer by the herpes vector G207
Prieto et al. Gene therapy of liver diseases
Prieto et al. The promise of gene therapy in gastrointestinal and liver diseases
CA2917584C (en) Genetically stable oncolytic rna virus, method of manufacturing and use thereof
JP3867968B2 (ja) 腫瘍細胞において選択的に増殖する腫瘍融解ウイルス
WO2003080083A1 (es) Uso de adenovirus mutados en los genes va para el tratamiento del cancer
Gao et al. Gene therapy: will the promise of optimizing lung allografts become reality?
Sorace et al. Enhancement of adenovirus delivery after ultrasound-stimulated therapy in a cancer model
JP2002515442A (ja) 癌を処置するためのアデノウィルス−化学治療剤のコンビネーション
Yang et al. A new recombinant vaccinia with targeted deletion of three viral genes: its safety and efficacy as an oncolytic virus
Zheng et al. Effects of extracellular vesicles derived from mesenchymal stem/stromal cells on liver diseases
Kuroda et al. Successful extracorporeal membranous oxygenation for a patient with life-threatening transfusion-related acute lung injury
Nawa et al. Gene directed enzyme prodrug therapy for ovarian cancer: could GDEPT become a promising treatment against ovarian cancer?
Jiang et al. Combining conditionally replicating adenovirus‐mediated gene therapy with chemotherapy: A novel antitumor approach
Fujita et al. Sendai virus-mediated gene delivery into hepatocytes via isolated hepatic perfusion
CN102229961A (zh) 小肽修饰表达两个外源基因的条件复制型溶瘤腺病毒载体及构建方法和应用