ES8506087A1 - Procedimiento para preparar proteina de uroquinasa humana bioactiva - Google Patents

Procedimiento para preparar proteina de uroquinasa humana bioactiva

Info

Publication number
ES8506087A1
ES8506087A1 ES533924A ES533924A ES8506087A1 ES 8506087 A1 ES8506087 A1 ES 8506087A1 ES 533924 A ES533924 A ES 533924A ES 533924 A ES533924 A ES 533924A ES 8506087 A1 ES8506087 A1 ES 8506087A1
Authority
ES
Spain
Prior art keywords
protein
human urokinase
polypeptides
preparation
functional human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES533924A
Other languages
English (en)
Other versions
ES533924A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of ES533924A0 publication Critical patent/ES533924A0/es
Publication of ES8506087A1 publication Critical patent/ES8506087A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Engineering & Computer Science (AREA)
  • Vascular Medicine (AREA)
  • Biotechnology (AREA)
  • Cardiology (AREA)
  • Diabetes (AREA)
  • Urology & Nephrology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PROCEDIMIENTO PARA PREPAR PROTEINA DE UROQUINASA HUMANA BIOACTIVA.CONSISTE EN UTILIZAR TECNOLOGIA DE ADN RECOMBINANTE/BIOQUIMICA DE PROTEINAS PARA PRODUCIR SATISFACTORIAMENTE UROQUINASA HUMANA EN LA FORMA DE ESPECIES BIOLOGIMANETES FUNCIONALES, HECHAS A MEDIDA. COMPRENDE DISOCIAR PROTEOLITICAMENTE IN VITRO UNA PROTEINA ESENCIALMENTE BIOINACTIVA, EN EL TERMINO N DE LA PROTEINA DE BAJO PESO MOLECULAR, REALIZANDO LA DISOCIACION CON TRIPSINA.
ES533924A 1982-04-15 1984-07-02 Procedimiento para preparar proteina de uroquinasa humana bioactiva Expired ES8506087A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36877382A 1982-04-15 1982-04-15
US47493083A 1983-03-14 1983-03-14

Publications (2)

Publication Number Publication Date
ES533924A0 ES533924A0 (es) 1985-07-01
ES8506087A1 true ES8506087A1 (es) 1985-07-01

Family

ID=27004326

Family Applications (3)

Application Number Title Priority Date Filing Date
ES521418A Expired ES8500325A1 (es) 1982-04-15 1983-04-13 Procedimiento para la preparacion de proteinas funcionales de uroquinasa humana.
ES533925A Granted ES533925A0 (es) 1982-04-15 1984-07-02 Procedimiento para producir un vehiculo de expresion para la formacion de uroquinasa humana
ES533924A Expired ES8506087A1 (es) 1982-04-15 1984-07-02 Procedimiento para preparar proteina de uroquinasa humana bioactiva

Family Applications Before (2)

Application Number Title Priority Date Filing Date
ES521418A Expired ES8500325A1 (es) 1982-04-15 1983-04-13 Procedimiento para la preparacion de proteinas funcionales de uroquinasa humana.
ES533925A Granted ES533925A0 (es) 1982-04-15 1984-07-02 Procedimiento para producir un vehiculo de expresion para la formacion de uroquinasa humana

Country Status (20)

Country Link
EP (2) EP0620279B1 (es)
JP (3) JPH0655145B2 (es)
AT (2) ATE112802T1 (es)
AU (1) AU573523B2 (es)
BR (1) BR8301918A (es)
CA (1) CA1341301C (es)
DE (2) DE3382760T2 (es)
DK (1) DK173910B1 (es)
ES (3) ES8500325A1 (es)
FI (1) FI88932C (es)
GR (1) GR78180B (es)
HK (1) HK38297A (es)
IE (1) IE64536B1 (es)
IL (2) IL68366A (es)
MY (1) MY102472A (es)
NO (1) NO831317L (es)
NZ (1) NZ203869A (es)
PH (4) PH24783A (es)
PT (1) PT76546B (es)
YU (1) YU45128B (es)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
AU587960B2 (en) * 1983-06-24 1989-09-07 Genentech Inc. Procaryotic carbonyl hydrolases
EP0139447B1 (en) * 1983-09-13 1992-12-30 The Green Cross Corporation A process for preparing urokinase zymogen
JPS61177987A (ja) * 1985-01-31 1986-08-09 Green Cross Corp:The ヒトウロキナ−ゼの製造方法
JPS60180591A (ja) * 1984-02-27 1985-09-14 Green Cross Corp:The ヒトウロキナ−ゼのｄνａ配列，プラスミド，宿主
DE3585097D1 (de) * 1984-02-27 1992-02-20 Green Cross Corp Herstellung menschlicher urokinase.
JPS61181377A (ja) * 1985-01-25 1986-08-14 Sagami Chem Res Center ヒトウロキナ−ゼ遺伝子
EP0210279B1 (en) * 1985-01-25 1991-08-14 Sagami Chemical Research Center Stabilized human prourokinase
US5219569A (en) * 1985-04-22 1993-06-15 Genentech, Inc. Protease resistant urokinase
US4916071A (en) 1985-08-14 1990-04-10 American Home Products Corporation Poly-kringle plasminogen activator
US4997766A (en) * 1986-07-11 1991-03-05 American Home Products Corporation Poly-kringle plasminogen activator
JPS62149625A (ja) * 1985-12-25 1987-07-03 Green Cross Corp:The 生理活性物質の製造方法
DE3613401A1 (de) * 1986-04-21 1987-12-17 Boehringer Mannheim Gmbh Verfahren zur herstellung von plasminogen-aktivatoren in prokaryonten
NL8601240A (nl) * 1986-05-15 1987-12-01 Leuven Res & Dev Vzw Plasminogeenactivator en trombolytisch geneesmiddel.
GB8623823D0 (en) * 1986-10-03 1986-11-05 Sandoz Ltd Therapeutic lysis of fibrin blood clots
JPS63105675A (ja) * 1986-10-23 1988-05-10 Green Cross Corp:The ヒトウロキナ−ゼの製造方法
ZA879286B (en) 1986-12-16 1988-10-26 Smith Kline Rit New plasminogen activators
EP0299053A1 (en) * 1987-01-30 1989-01-18 American Home Products Corporation Des-epidermal growth factor plasminogen activators
MY103358A (en) * 1987-04-15 1993-06-30 Novartis Ag Process for the production of protiens.
EP0330700B1 (en) * 1987-08-19 1993-12-22 Sagami Chemical Research Center Human prourokinase-like polypeptide
ES2061585T3 (es) * 1987-10-02 1994-12-16 Zymogenetics Inc Señal de secrecion de bar1.
AU2354588A (en) * 1987-10-09 1989-04-20 Collaborative Research Inc. Modified low molecular weight plasminogen activator and method of preparation
US4999194A (en) * 1988-01-14 1991-03-12 Collaborative Research, Inc. Two-chain urokinase plasminogen activators for treatment of thrombotic disease
US4920051A (en) * 1988-02-03 1990-04-24 Damon Biotech, Inc. Recovery of urokinase compounds
IE901849A1 (en) 1989-07-19 1991-06-19 Gruenenthal Gmbh Plasmids, their preparation and their use in the manufacture¹of a plasminogen activator
AU6470590A (en) 1989-10-23 1991-04-26 Smithkline Beecham Corporation Cyclic anti-aggregatory peptides
EP0897987B1 (en) * 1989-10-24 2001-02-28 Chiron Corporation Secretion of human protein linked to gamma interferon signal peptide
DE4101736A1 (de) 1991-01-22 1992-07-23 Gruenenthal Gmbh Neue als plasminogenaktivatoren einsetzbare polypeptide, dafuer codierende plasmide und verfahren zu deren herstellung und deren verwendung
US5888814A (en) * 1994-06-06 1999-03-30 Chiron Corporation Recombinant host cells encoding TNF proteins
WO2000000624A1 (en) * 1998-06-26 2000-01-06 Croptech Development Corporation Production of urokinase in plant-based expression systems
WO2001097752A2 (en) * 2000-06-20 2001-12-27 The Trustees Of The University Of Pennsylvania Compositions comprising urokinase for modulating muscle contractility and angiogenisis
US20030040095A1 (en) * 2001-03-16 2003-02-27 Achille Arini Method for the production of pharmaceutically active recombinant proteins
MX2008004693A (es) 2005-10-21 2008-09-03 Catalyst Biosciences Inc Proteasas modificadas que inhiben la activacion del complemento.
NZ596269A (en) 2006-07-05 2012-12-21 Catalyst Biosciences Inc Protease screening methods and proteases identified thereby

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0734743B2 (ja) * 1979-11-13 1995-04-19 バスキユラー・ラボラトリー・インコーポレーテツド プラスミノーゲン活性化剤
US4321363A (en) * 1980-02-21 1982-03-23 Nippon Soda Company Limited Adsorbent for urokinase containing agarose
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
US4506013A (en) * 1980-10-03 1985-03-19 Eli Lilly And Company Stabilizing and selecting recombinant DNA host cells
DE3479520D1 (en) * 1983-11-21 1989-09-28 Ciba Geigy Ag Synthesis of tissue plasminogen activator(tpa) by yeast

Also Published As

Publication number Publication date
PH24783A (en) 1990-10-30
EP0092182A3 (en) 1984-07-18
AU1352483A (en) 1983-10-20
YU45128B (en) 1992-03-10
JPS5951300A (ja) 1984-03-24
ATE232237T1 (de) 2003-02-15
FI88932B (fi) 1993-04-15
PH25312A (en) 1991-04-30
MY102472A (en) 1992-06-30
ATE112802T1 (de) 1994-10-15
IL83236A0 (en) 1987-12-31
DE3382760D1 (de) 1994-11-17
ES521418A0 (es) 1984-11-01
EP0620279B1 (en) 2003-02-05
NZ203869A (en) 1987-04-30
JPH0655145B2 (ja) 1994-07-27
EP0092182A2 (en) 1983-10-26
DE3382760T2 (de) 1995-03-02
YU86883A (en) 1988-10-31
CA1341301C (en) 2001-10-09
JPH0824579B2 (ja) 1996-03-13
DK163683D0 (da) 1983-04-14
IE950235L (en) 1983-10-15
DK173910B1 (da) 2002-02-11
IE830850L (en) 1983-10-15
ES533924A0 (es) 1985-07-01
EP0620279A1 (en) 1994-10-19
ES8602121A1 (es) 1985-12-01
BR8301918A (pt) 1983-12-20
PH25120A (en) 1991-02-19
DK163683A (da) 1983-11-28
FI831228L (fi) 1983-10-16
DE3382837T2 (de) 2004-01-15
DE3382837D1 (de) 2003-03-13
JPH0530970A (ja) 1993-02-09
IL68366A0 (en) 1983-07-31
JPH07186A (ja) 1995-01-06
IL68366A (en) 1990-08-31
PT76546B (en) 1986-03-20
JP2584192B2 (ja) 1997-02-19
NO831317L (no) 1983-10-17
AU573523B2 (en) 1988-06-16
PT76546A (en) 1983-05-01
HK38297A (en) 1997-04-04
FI831228A0 (fi) 1983-04-12
FI88932C (fi) 1993-07-26
IE64536B1 (en) 1995-08-09
ES8500325A1 (es) 1984-11-01
ES533925A0 (es) 1985-12-01
PH22461A (en) 1988-09-12
EP0092182B1 (en) 1994-10-12
GR78180B (es) 1984-09-26

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