GR3023287T3 - Enzymatic synthesis of oligonucleotides - Google Patents

Enzymatic synthesis of oligonucleotides

Info

Publication number: GR3023287T3
Authority: GR; Greece
Prior art keywords: template; sequence; present; oligonucleotides; catalytic
Prior art date: 1990-09-21

Application number

GR970400952T

Other languages

English (en)

Inventor

Rodney M Richards

Original Assignee

Amgen Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-09-21

Filing date

1997-04-29

Publication date

1997-07-30

1997-04-29 Application filed by Amgen Inc filed Critical Amgen Inc

1997-07-30 Publication of GR3023287T3 publication Critical patent/GR3023287T3/el

Links

108091034117 Oligonucleotide Proteins 0.000 title abstract 3
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 2
230000015572 biosynthetic process Effects 0.000 title 1
230000002255 enzymatic effect Effects 0.000 title 1
238000003786 synthesis reaction Methods 0.000 title 1
238000000034 method Methods 0.000 abstract 4
230000003197 catalytic effect Effects 0.000 abstract 2
239000003795 chemical substances by application Substances 0.000 abstract 2
230000035945 sensitivity Effects 0.000 abstract 2
230000003321 amplification Effects 0.000 abstract 1
238000006243 chemical reaction Methods 0.000 abstract 1
238000004519 manufacturing process Methods 0.000 abstract 1
238000003199 nucleic acid amplification method Methods 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
239000002777 nucleoside Substances 0.000 abstract 1
-1 nucleoside triphosphates Chemical class 0.000 abstract 1
238000006116 polymerization reaction Methods 0.000 abstract 1
239000001226 triphosphate Substances 0.000 abstract 1
235000011178 triphosphate Nutrition 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Genetics & Genomics (AREA)
General Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Biophysics (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Biomedical Technology (AREA)
Chemical Kinetics & Catalysis (AREA)
Crystallography & Structural Chemistry (AREA)
Plant Pathology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

GR970400952T 1990-09-21 1997-04-29 Enzymatic synthesis of oligonucleotides GR3023287T3 (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US58636890A	1990-09-21	1990-09-21
US76214091A	1991-09-23	1991-09-23
PCT/US1991/006887 WO1992005287A1 (en)	1990-09-21	1991-09-23	Enzymatic synthesis of oligonucleotides

Publications (1)

Publication Number	Publication Date
GR3023287T3 true GR3023287T3 (en)	1997-07-30

Family

ID=27079687

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
GR970400952T GR3023287T3 (en)	1990-09-21	1997-04-29	Enzymatic synthesis of oligonucleotides

Country Status (11)

Country	Link
US (1)	US5650271A (el)
EP (1)	EP0502180B1 (el)
JP (1)	JPH05502594A (el)
AT (1)	ATE151815T1 (el)
AU (1)	AU648842B2 (el)
CA (1)	CA2069096A1 (el)
DE (1)	DE69125702T2 (el)
DK (1)	DK0502180T3 (el)
ES (1)	ES2100963T3 (el)
GR (1)	GR3023287T3 (el)
WO (1)	WO1992005287A1 (el)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5645987A (en) *	1990-09-21	1997-07-08	Amgen Inc.	Enzymatic synthesis of oligonucleotides
WO1993019202A2 (en) *	1992-03-10	1993-09-30	The United States Of America, As Represented By The Secretary, Department Of Health & Human Services	Exchangeable template reaction
US5605798A (en)	1993-01-07	1997-02-25	Sequenom, Inc.	DNA diagnostic based on mass spectrometry
US5648211A (en) *	1994-04-18	1997-07-15	Becton, Dickinson And Company	Strand displacement amplification using thermophilic enzymes
US5830655A (en)	1995-05-22	1998-11-03	Sri International	Oligonucleotide sizing using cleavable primers
US5700642A (en) *	1995-05-22	1997-12-23	Sri International	Oligonucleotide sizing using immobilized cleavable primers
US5916777A (en) *	1995-06-07	1999-06-29	Gen-Probe Incorporated	Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
US5932450A (en) *	1995-06-07	1999-08-03	Gen-Probe Incorporated	Enzymatic synthesis of oligonucleotides using digestible templates
CA2222510A1 (en) *	1995-06-07	1996-12-19	Gen-Probe Incorporated	Template and primer based synthesis of enzymatically cleavable oligonucleotides
US5652126A (en) *	1995-06-07	1997-07-29	Gen-Probe Incorporated	Use of restriction endonuclease sequences for cleaving phosphorothioate oligonucleotides
US5739311A (en) *	1995-06-07	1998-04-14	Gen-Probe Incorporated	Enzymatic synthesis of phosphorothioate oligonucleotides using restriction endonucleases
DE69738206T2 (de)	1996-11-06	2008-07-17	Sequenom, Inc., San Diego	DNA-Diagnostik mittels Massenspektrometrie
DE69735445T2 (de)	1996-12-10	2006-08-10	Sequenom, Inc., San Diego	Abspaltbare, nicht-flüchtige moleküle zur massenmarkierung
US6025133A (en) *	1996-12-30	2000-02-15	Gen-Probe Incorporated	Promoter-sequestered oligonucleoside and method of use
US20030165888A1 (en) *	2001-07-18	2003-09-04	Brown Bob D.	Oligonucleotide probes and primers comprising universal bases for diagnostic purposes
US6518017B1 (en) *	1997-10-02	2003-02-11	Oasis Biosciences Incorporated	Combinatorial antisense library
US20030104417A1 (en) *	1998-08-12	2003-06-05	Proteus S.A.	Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides
US6951719B1 (en) *	1999-08-11	2005-10-04	Proteus S.A.	Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
AU777910B2 (en) *	1999-04-08	2004-11-04	Gen-Probe Incorporated	Amplification and sequencing primer pairs and use thereof
CA2365984A1 (en) *	1999-04-08	2000-10-19	Oasis Biosciences, Inc.	Antisense oligonucleotides comprising universal and/or degenerate bases
US7125982B1 (en)	2001-12-05	2006-10-24	Frayne Consultants	Microbial production of nuclease resistant DNA, RNA, and oligo mixtures
US20070238108A1 (en) *	2006-04-07	2007-10-11	Agilent Technologies, Inc.	Validation of comparative genomic hybridization
EP3237641B1 (en) *	2014-12-24	2020-04-08	Abbott Laboratories	Detection method, composition and kit based on signal amplifier dna cascade reactions with target extension

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS59219235A (ja) *	1983-05-30	1984-12-10	Mitsui Toatsu Chem Inc	消化性潰瘍用剤
US4795700A (en) *	1985-01-25	1989-01-03	California Institute Of Technology	Nucleic acid probes and methods of using same
US4994368A (en) *	1987-07-23	1991-02-19	Syntex (U.S.A.) Inc.	Amplification method for polynucleotide assays
EP0365627B1 (en) *	1988-03-24	1993-12-22	University Of Iowa Research Foundation	Catalytic hybridization systems for the detection of nucleic acid sequences based on their activity as cofactors in catalytic reactions in which a complementary labeled nucleic acid probe is cleaved
US5132215A (en) *	1988-09-15	1992-07-21	Eastman Kodak Company	Method of making double-stranded dna sequences
AU649066B2 (en) *	1990-07-25	1994-05-12	Syngene, Inc.	Circular extension for generating multiple nucleic acid complements
US5455166A (en) *	1991-01-31	1995-10-03	Becton, Dickinson And Company	Strand displacement amplification

1991
- 1991-09-23 ES ES91919119T patent/ES2100963T3/es not_active Expired - Lifetime
- 1991-09-23 DK DK91919119.7T patent/DK0502180T3/da active
- 1991-09-23 AT AT91919119T patent/ATE151815T1/de not_active IP Right Cessation
- 1991-09-23 DE DE69125702T patent/DE69125702T2/de not_active Expired - Fee Related
- 1991-09-23 EP EP91919119A patent/EP0502180B1/en not_active Expired - Lifetime
- 1991-09-23 CA CA002069096A patent/CA2069096A1/en not_active Abandoned
- 1991-09-23 AU AU88446/91A patent/AU648842B2/en not_active Ceased
- 1991-09-23 WO PCT/US1991/006887 patent/WO1992005287A1/en not_active Ceased
- 1991-09-23 JP JP3517557A patent/JPH05502594A/ja active Pending
1993
- 1993-04-01 US US08/041,631 patent/US5650271A/en not_active Expired - Fee Related
1997
- 1997-04-29 GR GR970400952T patent/GR3023287T3/el unknown

Also Published As

Publication number	Publication date
EP0502180A1 (en)	1992-09-09
ES2100963T3 (es)	1997-07-01
CA2069096A1 (en)	1992-03-22
JPH05502594A (ja)	1993-05-13
HK1007170A1 (en)	1999-04-01
EP0502180A4 (en)	1993-03-17
DE69125702D1 (de)	1997-05-22
AU648842B2 (en)	1994-05-05
US5650271A (en)	1997-07-22
WO1992005287A1 (en)	1992-04-02
DK0502180T3 (da)	1997-09-22
AU8844691A (en)	1992-04-15
EP0502180B1 (en)	1997-04-16
DE69125702T2 (de)	1997-11-27
ATE151815T1 (de)	1997-05-15

Publication	Publication Date	Title
GR3023287T3 (en)	1997-07-30	Enzymatic synthesis of oligonucleotides
Sawai et al.	1989	Uranyl ion as a highly effective catalyst for internucleotide bond formation.
Agarwal et al.	1970	Total synthesis of the gene for an alanine transfer ribonucleic acid from yeast
Arai et al.	1981	Unique primed start of phage phi X174 DNA replication and mobility of the primosome in a direction opposite chain synthesis.
ES8800357A1 (es)	1987-10-16	Un procedimiento para sintetizar un fragmento de acido nucleico
ATE137760T1 (de)	1996-05-15	Verfahren zur herstellung von oligonucleotiden, anwendbar zu serienherstellung
UA49843C2 (uk)	2002-10-15	Олігонуклеотид для ампліфікації послідовностей нуклеїнових кислот, спосіб ампліфікації цільової послідовності нуклеїнової кислоти, набір для здійснення реакції ампліфікації нуклеїнової кислоти
ATE244259T1 (de)	2003-07-15	Verwendungen von fluoreszierenden n-nukleosiden und dessen analogen
CA2005589A1 (en)	1990-06-16	Self-sustained, sequence replication system
EP0395398A3 (en)	1991-09-18	Amplification of nucleic acid sequences using random sequence oligonucleotides as primers
Fairfield et al.	1983	On the processivity of DNA replication
DK0836612T3 (da)	2000-10-30	Nye synthoner til stereoselektiv oligonucleotidsyntese
ES454538A1 (es)	1977-12-01	Un proceso para realizar una reaccion.
Letsinger et al.	1972	Nucleotide chemistry. XVII. Enzymic synthesis of polydeoxyribonucleotides possessing internucleotide phosphoramidate bonds
ATE130634T1 (de)	1995-12-15	Enzymatisches amplifizierungsverfahren in vitro.
Rammler	1971	Periodate oxidations of enamines. I. Oxidation of adenosine 5'-monophosphate in the presence of methylamine
ES470340A1 (es)	1979-09-16	Un metodo para obtener una cepa de microorganismos modifica-dos para contener un fragmento de una secuencia especifica de desoxiribonucleotidos
ATE137807T1 (de)	1996-05-15	Systeme für amplifikation/nachweis von nukleinsäuren
GB1385164A (en)	1975-02-26	Process and catalyst for the polymerization of olefins
ES453775A1 (es)	1977-11-16	Procedimiento para la preparacion de n,n'-bis-trimetilsili- lurea.
HUT63432A (en)	1993-08-30	Process for cemically producing oliginucletides
NL7203123A (en)	1972-06-26	Riboside derivs from bacillus megaterium
ES390618A1 (es)	1973-06-16	Procedimiento para la polimerizacion en masa de acriloni- trilo.
Maitra	1970	The identification of 3′-hydroxyl nucleoside terminus of RNA chains released in the DNA-dependent RNA polymerase reaction
AU5337690A (en)	1990-10-09	Method for stabilizing the hybridization of complementary polynucleotide sequences