ATE130634T1 - Enzymatisches amplifizierungsverfahren in vitro. - Google Patents

Enzymatisches amplifizierungsverfahren in vitro.

Info

Publication number: ATE130634T1
Authority: AT; Austria
Prior art keywords: amplification; vitro; reamplification; pcr reaction; resulting
Prior art date: 1990-06-29

Application number

AT91911799T

Other languages

English (en)

Inventor

Pascal Brandys

Auriol Luc D

Original Assignee

Genset Sa

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-06-29

Filing date

1991-06-27

Publication date

1995-12-15

1991-06-27 Application filed by Genset Sa filed Critical Genset Sa

1995-12-15 Application granted granted Critical

1995-12-15 Publication of ATE130634T1 publication Critical patent/ATE130634T1/de

Links

230000003321 amplification Effects 0.000 title abstract 6
238000003199 nucleic acid amplification method Methods 0.000 title abstract 6
238000000034 method Methods 0.000 title abstract 4
238000000338 in vitro Methods 0.000 title abstract 2
230000002255 enzymatic effect Effects 0.000 title 1
108091034117 Oligonucleotide Proteins 0.000 abstract 2
108020004414 DNA Proteins 0.000 abstract 1
102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Saccharide Compounds (AREA)

AT91911799T 1990-06-29 1991-06-27 Enzymatisches amplifizierungsverfahren in vitro. ATE130634T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
FR9008247A FR2663949A1 (fr)	1990-06-29	1990-06-29	Perfectionnements a un procede d'amplification enzymatique in vitro.

Publications (1)

Publication Number	Publication Date
ATE130634T1 true ATE130634T1 (de)	1995-12-15

Family

ID=9398171

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT91911799T ATE130634T1 (de)	1990-06-29	1991-06-27	Enzymatisches amplifizierungsverfahren in vitro.

Country Status (6)

Country	Link
EP (1)	EP0536214B1 (de)
AT (1)	ATE130634T1 (de)
AU (1)	AU8092791A (de)
DE (1)	DE69114844T2 (de)
FR (1)	FR2663949A1 (de)
WO (1)	WO1992000384A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6589734B1 (en)	1989-07-11	2003-07-08	Gen-Probe Incorporated	Detection of HIV
US5856088A (en) *	1989-07-11	1999-01-05	Gen-Probe Incorporated	Detection of human immunodeficiency virus type 1
US5650302A (en) *	1990-05-01	1997-07-22	Amgen Inc.	Method for reducing carryover contamination in an amplification procedure
CA2058232A1 (en) *	1991-01-22	1992-07-23	Joseph A. Walder	Process to prevent contamination by amplified nucleic acid sequence
CA2101119A1 (en) *	1992-08-17	1994-02-18	George T. Walker	Exonuclease decontamination method
WO1994023069A1 (en) *	1993-03-26	1994-10-13	Gen-Probe Incorporated	Detection of human immunodeficiency virus type 1
US5605796A (en) *	1994-07-19	1997-02-25	Behringwerke Ag	Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conjugates
ZA956776B (en)	1994-08-25	1996-03-20	Akzo Nobel Nv	A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
CA2176193A1 (en) *	1995-05-22	1996-11-23	John Wesley Backus	Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
FR2737223B1 (fr) *	1995-07-24	1997-09-12	Bio Merieux	Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
US6495320B1 (en) *	1999-07-21	2002-12-17	Affymetrix, Inc.	Even length proportional amplification of nucleic acids

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4683202A (en)	1985-03-28	1987-07-28	Cetus Corporation	Process for amplifying nucleic acid sequences
JP2856804B2 (ja) *	1988-03-24	1999-02-10	ユニバーシティオブアイオワリサーチファウンデイション	標識された相補的核酸プローブを開裂する触媒反応の補因子としての活性に基づく核酸配列検出のための触媒ハイブリッド形成系
DE68928853T2 (de) *	1988-05-20	1999-08-05	Cetus Corp., Emeryville, Calif.	Befestigung von sequenzspezifischen proben
AU4181089A (en) *	1988-08-01	1990-03-05	George D. Cimino	Identification of allele specific nucleic acid sequences by hybridization with crosslinkable oligonucleotide probes
CA2004574A1 (en) *	1988-12-05	1990-06-05	Barbara C. Chu	Target nucleic acid amplification/detection systems
AU5358990A (en) *	1989-03-21	1990-10-22	Collaborative Research Inc.	Multiplex dna diagnostic test
US5035996A (en) *	1989-06-01	1991-07-30	Life Technologies, Inc.	Process for controlling contamination of nucleic acid amplification reactions
FR2649122B1 (fr) *	1989-07-03	1991-10-18	Pasteur Institut	Perfectionnements apportes aux techniques d'amplification par reaction de polymerisation en chaine (pcr)
DK0415755T3 (da) *	1989-09-01	1996-01-22	Life Technologies Inc	Fremgangsmåde til bekæmpelse af kontaminering af oligonukleotidafhængige nukleinsyreamplifikationsreaktioner

1990
- 1990-06-29 FR FR9008247A patent/FR2663949A1/fr active Granted
1991
- 1991-06-27 WO PCT/FR1991/000513 patent/WO1992000384A1/fr not_active Ceased
- 1991-06-27 DE DE69114844T patent/DE69114844T2/de not_active Expired - Fee Related
- 1991-06-27 EP EP91911799A patent/EP0536214B1/de not_active Expired - Lifetime
- 1991-06-27 AT AT91911799T patent/ATE130634T1/de not_active IP Right Cessation
- 1991-06-27 AU AU80927/91A patent/AU8092791A/en not_active Abandoned

Also Published As

Publication number	Publication date
AU8092791A (en)	1992-01-23
EP0536214B1 (de)	1995-11-22
FR2663949A1 (fr)	1992-01-03
DE69114844D1 (de)	1996-01-04
WO1992000384A1 (fr)	1992-01-09
FR2663949B1 (de)	1995-01-20
EP0536214A1 (de)	1993-04-14
DE69114844T2 (de)	1996-08-01

Legal Events

Date	Code	Title	Description
1996-05-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
Schiebel et al.	1993	RNA-directed RNA polymerase from tomato leaves. II. Catalytic in vitro properties
ES2108748T3 (es)	1998-01-01	Amplificacion de acidos nucleicos con actividad de rna polimerasa dependiente de dna, de rna replicasas.
UA49843C2 (uk)	2002-10-15	Олігонуклеотид для ампліфікації послідовностей нуклеїнових кислот, спосіб ампліфікації цільової послідовності нуклеїнової кислоти, набір для здійснення реакції ампліфікації нуклеїнової кислоти
DE3856455D1 (de)	2001-03-22	Selektive Amplifikation von Oligonukleotidenzielsequenzen
DK0721988T3 (da)	2000-07-24	Kimærisk oligonucleotid og dets anvendelse ved opnåelse af transkripter af en nucleinsyre
DK1300466T3 (da)	2006-08-28	En kaskade nukleinsyre amplifikationsreaktion
ATE206467T1 (de)	2001-10-15	'terminal repeat'- amplifikation
ATE130634T1 (de)	1995-12-15	Enzymatisches amplifizierungsverfahren in vitro.
MX9705961A (es)	1998-02-28	Polimerasa de adn termoestable, modificada.
ATE161587T1 (de)	1998-01-15	Technik des genetischen fingerabdrucks mit dns- vervielfältigung
CA2082842A1 (en)	1993-05-20	Nucleic acid target generation
EP0395398A3 (de)	1991-09-18	Verstärkung von Nukleinsäure-Sequenzen unter Verwendung von Stichsequenz-Oligonukleotiden als Primer
KR920701478A (ko)	1992-08-11	핵산서열의 증폭방법
JO1598B1 (en)	1989-12-16	A method for amplifying and amplifying the biocleotide sequences
ATE251674T1 (de)	2003-10-15	Verfahren, reagenz und salz zum nachweis und amplifikation von nukleinsäuresequenzen
DK0502180T3 (da)	1997-09-22	Enzymatisk syntese af oligonukleotider
ATE361377T1 (de)	2007-05-15	Leistungstarke verknüpfung von nukleinsäuresegmenten
GR3037030T3 (en)	2002-01-31	Method for detecting a target nucleic acid
SE9403805D0 (sv)	1994-11-07	Method of preparing oligonucleotide probes or primers, vector therefor and use thereof
EP1103624A4 (de)	2004-12-22	Verbessertes verfahren zur amplifikation von nukleinsäuren
EP0989192A3 (de)	2004-02-25	Verfahren zur Nucleinsäuresynthese
DE59814461D1 (de)	2010-09-16	Verfahren zur synthese und amplifikation von nukleinsäuren
DE50209094D1 (de)	2007-02-08	Vermehrung von ribonukleinsäuren
MY111893A (en)	2001-02-28	Method for detecting a target nucleic acid
MX9706988A (es)	1998-08-30	Procedimiento para la amplificacion de acidos nucleicos.