ES2100963T3 - Sintesis enzimatica de oligonucleotidos. - Google Patents

Sintesis enzimatica de oligonucleotidos.

Info

Publication number
ES2100963T3
ES2100963T3 ES91919119T ES91919119T ES2100963T3 ES 2100963 T3 ES2100963 T3 ES 2100963T3 ES 91919119 T ES91919119 T ES 91919119T ES 91919119 T ES91919119 T ES 91919119T ES 2100963 T3 ES2100963 T3 ES 2100963T3
Authority
ES
Spain
Prior art keywords
template
sequence
present
oligonucleotides
catalytic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91919119T
Other languages
English (en)
Inventor
Rodney M Richards
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Application granted granted Critical
Publication of ES2100963T3 publication Critical patent/ES2100963T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

LA PRESENTE INVENCION HACE POSIBLE LA PRODUCCION CATALITICA DE OLIGONUCLEOTIDOS DE SECUENCIA ESPECIFICA MEDIANTE EL USO DE UNA SECUENCIA MODELO ESPECIALMENTE DISEÑADA. SE PUEDE HACER QUE LA REACCION SE DESARROLLE ISOTERMICAMENTE ANTE LA PRESENCIA DE UN EXCEDENTE DE TRIFOSFATOS NUCLEOSIDOS, UN AGENTE PARA LA POLIMERIZACION Y UN AGENTE CORTANTE. PUESTO QUE EL PROCESO ES CATALITICO RESPECTO A LA SECUENCIA MODELO, SE PUEDE GENERAR TEORICAMENTE UNA CANTIDAD ILIMITADA DE UN PRODUCTO OLIGONUCLEOTIDO A PARTIR DE UNA SOLA MOLECULA MODELO. DONDE SE INICIA EL PROCESO POR LA PRESENCIA DE UNA SECUENCIA DE BLANCO DE ACIDO NUCLEICO, EL METODO DE LA PRESENTE INVENCION SE PUEDE UTILIZAR CON FINES DE DIAGNOSTICO COMO UN METODO DE AMPLIFICACION PARA MEJORAR LA SENSIBILIDAD. LA SENSIBILIDAD DEL DIAGNOSTICO SE PUEDE MEJORAR AUN MAS SI SE UTILIZA UNA CASCADA DE ESTAS SECUENCIAS MODELO.
ES91919119T 1990-09-21 1991-09-23 Sintesis enzimatica de oligonucleotidos. Expired - Lifetime ES2100963T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US58636890A 1990-09-21 1990-09-21
US76214091A 1991-09-23 1991-09-23
PCT/US1991/006887 WO1992005287A1 (en) 1990-09-21 1991-09-23 Enzymatic synthesis of oligonucleotides

Publications (1)

Publication Number Publication Date
ES2100963T3 true ES2100963T3 (es) 1997-07-01

Family

ID=27079687

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91919119T Expired - Lifetime ES2100963T3 (es) 1990-09-21 1991-09-23 Sintesis enzimatica de oligonucleotidos.

Country Status (11)

Country Link
US (1) US5650271A (es)
EP (1) EP0502180B1 (es)
JP (1) JPH05502594A (es)
AT (1) ATE151815T1 (es)
AU (1) AU648842B2 (es)
CA (1) CA2069096A1 (es)
DE (1) DE69125702T2 (es)
DK (1) DK0502180T3 (es)
ES (1) ES2100963T3 (es)
GR (1) GR3023287T3 (es)
WO (1) WO1992005287A1 (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5645987A (en) * 1990-09-21 1997-07-08 Amgen Inc. Enzymatic synthesis of oligonucleotides
AU672969B2 (en) * 1992-03-10 1996-10-24 United States of America, as represented by The Secretary, Department of Health & Human Services, The Exchangeable template reaction
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5700642A (en) * 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
JPH11513881A (ja) * 1995-06-07 1999-11-30 ジェン−プロープ・インコーポレーテッド テンプレートおよびプライマーに基づく酵素的に切断可能なオリゴヌクレオチドの合成
US5932450A (en) * 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
US5739311A (en) * 1995-06-07 1998-04-14 Gen-Probe Incorporated Enzymatic synthesis of phosphorothioate oligonucleotides using restriction endonucleases
US5652126A (en) * 1995-06-07 1997-07-29 Gen-Probe Incorporated Use of restriction endonuclease sequences for cleaving phosphorothioate oligonucleotides
EP1164203B1 (en) 1996-11-06 2007-10-10 Sequenom, Inc. DNA Diagnostics based on mass spectrometry
EP0963443B1 (en) 1996-12-10 2006-03-08 Sequenom, Inc. Releasable nonvolatile mass-label molecules
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
US6518017B1 (en) * 1997-10-02 2003-02-11 Oasis Biosciences Incorporated Combinatorial antisense library
US20030165888A1 (en) * 2001-07-18 2003-09-04 Brown Bob D. Oligonucleotide probes and primers comprising universal bases for diagnostic purposes
US6951719B1 (en) * 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US20030104417A1 (en) * 1998-08-12 2003-06-05 Proteus S.A. Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides
DE60023480T2 (de) * 1999-04-08 2006-07-20 Gen-Probe Inc., San Diego Amplifikation und sequenzierung von primer-paaren und deren verwendung
CA2365984A1 (en) * 1999-04-08 2000-10-19 Oasis Biosciences, Inc. Antisense oligonucleotides comprising universal and/or degenerate bases
US7125982B1 (en) 2001-12-05 2006-10-24 Frayne Consultants Microbial production of nuclease resistant DNA, RNA, and oligo mixtures
US20070238108A1 (en) * 2006-04-07 2007-10-11 Agilent Technologies, Inc. Validation of comparative genomic hybridization
US10604790B2 (en) * 2014-12-24 2020-03-31 Abbott Laboratories Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59219235A (ja) * 1983-05-30 1984-12-10 Mitsui Toatsu Chem Inc 消化性潰瘍用剤
US4795700A (en) * 1985-01-25 1989-01-03 California Institute Of Technology Nucleic acid probes and methods of using same
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
JP2856804B2 (ja) * 1988-03-24 1999-02-10 ユニバーシティ オブ アイオワ リサーチ ファウンデイション 標識された相補的核酸プローブを開裂する触媒反応の補因子としての活性に基づく核酸配列検出のための触媒ハイブリッド形成系
US5132215A (en) * 1988-09-15 1992-07-21 Eastman Kodak Company Method of making double-stranded dna sequences
AU649066B2 (en) * 1990-07-25 1994-05-12 Syngene, Inc. Circular extension for generating multiple nucleic acid complements
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification

Also Published As

Publication number Publication date
DE69125702T2 (de) 1997-11-27
EP0502180A4 (en) 1993-03-17
WO1992005287A1 (en) 1992-04-02
EP0502180B1 (en) 1997-04-16
US5650271A (en) 1997-07-22
AU648842B2 (en) 1994-05-05
DE69125702D1 (de) 1997-05-22
HK1007170A1 (en) 1999-04-01
DK0502180T3 (da) 1997-09-22
CA2069096A1 (en) 1992-03-22
GR3023287T3 (en) 1997-07-30
EP0502180A1 (en) 1992-09-09
AU8844691A (en) 1992-04-15
JPH05502594A (ja) 1993-05-13
ATE151815T1 (de) 1997-05-15

Similar Documents

Publication Publication Date Title
ES2100963T3 (es) Sintesis enzimatica de oligonucleotidos.
Agarwal et al. Total synthesis of the gene for an alanine transfer ribonucleic acid from yeast
ES8800357A1 (es) Un procedimiento para sintetizar un fragmento de acido nucleico
Sokolova et al. Chemical reactions within DNA duplexes cyanogen bromide as an effective oligodeoxyribonucleotide coupling agent
Sawai et al. Uranyl ion as a highly effective catalyst for internucleotide bond formation.
DE69026963D1 (de) Verfahren zur herstellung von oligonucleotiden, anwendbar zu serienherstellung
ATE244259T1 (de) Verwendungen von fluoreszierenden n-nukleosiden und dessen analogen
KR920701478A (ko) 핵산서열의 증폭방법
UA49843C2 (uk) Олігонуклеотид для ампліфікації послідовностей нуклеїнових кислот, спосіб ампліфікації цільової послідовності нуклеїнової кислоти, набір для здійснення реакції ампліфікації нуклеїнової кислоти
DK0919565T4 (da) Mærkede oligonukleotider
DE68928222D1 (de) 4 Selbstfortsetzendes System für Sequenzreplikation
NO895090D0 (no) Fremgangsmaate og system for deteksjonsdiagnostisering.
ATE178946T1 (de) Verbessertes verfahren für die quantifizierung von nukleinsäuren
Fairfield et al. On the processivity of DNA replication
DK0836612T3 (da) Nye synthoner til stereoselektiv oligonucleotidsyntese
Letsinger et al. Nucleotide chemistry. XVII. Enzymic synthesis of polydeoxyribonucleotides possessing internucleotide phosphoramidate bonds
ATE130634T1 (de) Enzymatisches amplifizierungsverfahren in vitro.
ES470340A1 (es) Un metodo para obtener una cepa de microorganismos modifica-dos para contener un fragmento de una secuencia especifica de desoxiribonucleotidos
Rammler Periodate oxidations of enamines. I. Oxidation of adenosine 5'-monophosphate in the presence of methylamine
DE68926460D1 (de) Systeme für amplifikation/nachweis von nukleinsäuren
ES453775A1 (es) Procedimiento para la preparacion de n,n'-bis-trimetilsili- lurea.
Rowe et al. Synthesis of 3′–5′ dinucleotides with RNase T1
DE60021875D1 (de) Verfahren zur herstellung von nukleosid-verbindung
DE59103277D1 (de) Verfahren zur chemischen Synthese von Oligonukleotiden.
NL7203123A (en) Riboside derivs from bacillus megaterium

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 502180

Country of ref document: ES