HK1047603B - Nucleic acid detection methods using universal priming - Google Patents

Nucleic acid detection methods using universal priming Download PDF

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Publication number
HK1047603B
HK1047603B HK02109139.1A HK02109139A HK1047603B HK 1047603 B HK1047603 B HK 1047603B HK 02109139 A HK02109139 A HK 02109139A HK 1047603 B HK1047603 B HK 1047603B
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Hong Kong
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target
probe
probes
sequence
beads
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HK02109139.1A
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German (de)
English (en)
Chinese (zh)
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HK1047603A1 (en
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Jian-Bing Fan
Mark S. Chee
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Illumina, Inc.
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Application filed by Illumina, Inc. filed Critical Illumina, Inc.
Publication of HK1047603A1 publication Critical patent/HK1047603A1/en
Publication of HK1047603B publication Critical patent/HK1047603B/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (5)

  1. Méthode de détermination de l'identification de nucléotides en des positions de détection dans des séquences cibles consistant à:
    a) prévoir un certain nombre de groupes de sondes, où chaque groupe comprend:
    i) une première sonde de ligature comprenant un site d'amorce universelle en amont et une première séquence spécifique de la cible; et
    ii) une seconde sonde de ligature comprenant un site d'amorce universelle en aval et une seconde séquence spécifique de la cible; où la première ou la seconde séquence spécifique de la cible comprend une base en une position d'interrogation et où au moins l'une desdites première et seconde sondes de ligature comprend une séquence d'adaptation complémentaire d'une sonde de capture sur une série;
    b) hybrider ladite quantité de groupes de sondes à des séquences cibles immobilisées sur un support ou des supports, lesdites séquences cibles comprenant chacune un premier domaine de cible comprenant une position de détection et un second domaine de cible adjacent à ladite position de détection, où une première sonde de ligature comprenant une séquence spécifique de la cible s'hybride au premier domaine de la cible et une seconde sonde de ligature comprenant une séquence spécifique de la cible s'hybride au second domaine de la cible et où si ladite base à ladite position d'interrogation est parfaitement complémentaire d'un nucléotide à ladite position de détection, un complexe de ligature se forme;
    c) prévoir une ligase qui lie lesdites première et seconde sondes de ligature pour former des sondes ligaturées;
    d) amplifier lesdites sondes ligaturées pour générer un certain nombre d'amplicons;
    e) mettre lesdits amplicons en contact avec une série comprenant des sondes de capture sur un support solide; et
    f) déterminer le nucléotide à chacune desdites positions de détection.
  2. Méthode de la revendication 1, où les amplicons comprennent un marqueur.
  3. Méthode de la revendication 2, où le marqueur comprend un marquer fluorescent.
  4. Méthode de l'une quelconque des revendications précédentes, où les premier et second domaines de la cible sont séparés par un ou plusieurs nucléotides et où la méthode comprend l'addition de dNTP et d'une polymérase avant de produire la ligase pour former la sonde ligaturée.
  5. Méthode de l'une quelconque des revendications précédentes, où les séquences de la cible sont de l'ADN génomique.
HK02109139.1A 2000-02-07 2001-02-07 Nucleic acid detection methods using universal priming HK1047603B (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US18081000P 2000-02-07 2000-02-07
US60/180,810 2000-02-07
US23473200P 2000-09-22 2000-09-22
US60/234,732 2000-09-22
PCT/US2001/004056 WO2001057269A2 (fr) 2000-02-07 2001-02-07 Procedes de detection d'acide nucleique par amorçage universel

Publications (2)

Publication Number Publication Date
HK1047603A1 HK1047603A1 (en) 2003-02-28
HK1047603B true HK1047603B (en) 2009-03-27

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Family Applications (1)

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HK02109139.1A HK1047603B (en) 2000-02-07 2001-02-07 Nucleic acid detection methods using universal priming

Country Status (10)

Country Link
US (5) US20020006617A1 (fr)
EP (2) EP1990428B1 (fr)
JP (1) JP2003521252A (fr)
AT (2) ATE492652T1 (fr)
AU (1) AU2001238068A1 (fr)
CA (1) CA2399733C (fr)
DE (2) DE60143723D1 (fr)
DK (1) DK1259643T3 (fr)
HK (1) HK1047603B (fr)
WO (1) WO2001057269A2 (fr)

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ATE411397T1 (de) 2008-10-15
WO2001057269A2 (fr) 2001-08-09
US20040224352A1 (en) 2004-11-11
HK1047603A1 (en) 2003-02-28
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JP2003521252A (ja) 2003-07-15
ATE492652T1 (de) 2011-01-15
US20040224353A1 (en) 2004-11-11
CA2399733A1 (fr) 2001-08-09
US20020006617A1 (en) 2002-01-17
DE60143723D1 (de) 2011-02-03
EP1259643A2 (fr) 2002-11-27
EP1990428B1 (fr) 2010-12-22
CA2399733C (fr) 2011-09-20
WO2001057269A3 (fr) 2002-07-18
EP1990428A1 (fr) 2008-11-12
US20020132241A1 (en) 2002-09-19
US20030003490A1 (en) 2003-01-02
DK1259643T3 (da) 2009-02-23
EP1259643B1 (fr) 2008-10-15

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