HUE032608T2 - Javított eljárás sejtek tenyésztésére - Google Patents

Javított eljárás sejtek tenyésztésére Download PDF

Info

Publication number
HUE032608T2
HUE032608T2 HUE13169694A HUE13169694A HUE032608T2 HU E032608 T2 HUE032608 T2 HU E032608T2 HU E13169694 A HUE13169694 A HU E13169694A HU E13169694 A HUE13169694 A HU E13169694A HU E032608 T2 HUE032608 T2 HU E032608T2
Authority
HU
Hungary
Prior art keywords
cells
cell culture
cell
biological substance
desired biological
Prior art date
Application number
HUE13169694A
Other languages
English (en)
Inventor
Gerben Meile Zijlstra
Robert Patrick Hof
Jacob Schilder
Original Assignee
Patheon Holdings I B V
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38441437&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HUE032608(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Patheon Holdings I B V filed Critical Patheon Holdings I B V
Publication of HUE032608T2 publication Critical patent/HUE032608T2/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/08—Hollow fibre membranes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Ophthalmology & Optometry (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Water Supply & Treatment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)

Claims (4)

  1. javúoit eljárás sejt# tenyésztésére Ss#>a|Ä! igÉiyböniok E Egy &9infc biológiái anyagot termeli emlős sejtek Muszgenziját tattelmmti s«|rmnyiszeL melynek életképes seitsürüsége legalább 50 x 1(L sep/ml és a kívánt biológiai anyag keneeni-ráció)a legalább 5 g/L, amely a sejteknek reaktorban sejtten\és/tó: kbkegbeá szusxpmzióban történd tenyésztésével állítható elő, áltól a sejtek a kívánt biológiai anyagot termelik, ahol legalább egy sejtteeyesztö közeg komponenst adagolónk a scjitenyészetbez, és altöba sejteket, e;kMni biológiai miyagnt és sejlteayésztO; közeget taríaínrazó sejttenyészetet egy szűri felett keringtetjük tangenciálís áramoltatás alkalmazásával, és aboi a szóró pornsméretét egy olyan molekulatömeg küszibéitékjél§étaezis mely kisebb, mint á kívánt biológiai anyag molekulatömege, a kívánt biológiai anyagnak: a kívánt biológiát anyagnál kisebb moléknlatótnegá anyagoktól történő elválasztásába^ aboi a szörörői élvezeteit folyadék lényegében csak; a kívánt biológiai an vápái; kisebb mole» kulatömegü anyagokat tartalmazza, és ahol a kívánt biológiai anyag a reaktorban marad, vagy oda visszavezetésre kerül.
  2. 2. .Az I, igénypont szétinti sejttenyészet, áltól a kívánt biológiái anyagot legalább egy, a seb tökbe CranszíéMált gén kódolja.
  3. 3, Az I < vagy 2, igénypont szerinti segtenyészeb aboi a kívánt: biológiai anyag koncentrációja legalább 10 g/L. á, Az lók Igénypntok bármelyiké szetmtl sejttenyészet ahol a kívánt biológiai anyag kom eenttáalója legalább 11 g/L. $;. Az: í~4, igénypontok bármelyike szerint! sejttenyészet, altöl: a Rivant biológiai anyag egy Igák Az 1-5. ig^övpoättak bármelyike szerinti sejtíeayészet, ahol az emlős sejtok El kmnortaiizáh HER sejtek. ?. A 6. igénypont szerinti seittenyészei, ahol m emlős sejtek FE11.C6 sejtek. k. Az !~7, igénypontok bármelyike szerinti sojttonyészet, ahol az életképes sejtek shfősége 50 a 10° seji/rnl és 20.0 a Kf sej i/ml között van,
  4. 9, Az I~8, igénypontok' bármely ike szerinti sejttenyészet, ahol m életképes sejtek sűrűsége 80 a 10° sejt/tm és 200 a ük sejiőtú között vart.
HUE13169694A 2006-07-14 2007-07-04 Javított eljárás sejtek tenyésztésére HUE032608T2 (hu)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06014671 2006-07-14
EP07002571 2007-02-06

Publications (1)

Publication Number Publication Date
HUE032608T2 true HUE032608T2 (hu) 2017-10-30

Family

ID=38441437

Family Applications (5)

Application Number Title Priority Date Filing Date
HUE13169693A HUE035109T2 (hu) 2006-07-14 2007-07-04 Javított eljárás sejtek tenyésztésére
HUE13169694A HUE032608T2 (hu) 2006-07-14 2007-07-04 Javított eljárás sejtek tenyésztésére
HUE13169685A HUE034299T2 (hu) 2006-07-14 2007-07-04 Javított eljárás sejtek tenyésztésére
HUE10183677A HUE028593T2 (hu) 2006-07-14 2007-07-04 Sejtek tenyésztésére szolgáló, javított eljárás
HUE07765049A HUE028365T2 (hu) 2006-07-14 2007-07-04 Javított eljárás sejtek tenyésztésére

Family Applications Before (1)

Application Number Title Priority Date Filing Date
HUE13169693A HUE035109T2 (hu) 2006-07-14 2007-07-04 Javított eljárás sejtek tenyésztésére

Family Applications After (3)

Application Number Title Priority Date Filing Date
HUE13169685A HUE034299T2 (hu) 2006-07-14 2007-07-04 Javított eljárás sejtek tenyésztésére
HUE10183677A HUE028593T2 (hu) 2006-07-14 2007-07-04 Sejtek tenyésztésére szolgáló, javított eljárás
HUE07765049A HUE028365T2 (hu) 2006-07-14 2007-07-04 Javított eljárás sejtek tenyésztésére

Country Status (22)

Country Link
US (7) US8119368B2 (hu)
EP (5) EP2634243B2 (hu)
JP (4) JP5092129B2 (hu)
KR (3) KR101685246B1 (hu)
CN (2) CN104263700A (hu)
AR (1) AR062072A1 (hu)
AU (1) AU2007272054C1 (hu)
BR (1) BRPI0714347A2 (hu)
CA (1) CA2657040C (hu)
CL (1) CL2007002060A1 (hu)
DK (4) DK2634242T3 (hu)
EA (2) EA016451B1 (hu)
ES (5) ES2625062T3 (hu)
FI (1) FI2343362T4 (hu)
HU (5) HUE035109T2 (hu)
IL (2) IL196322A0 (hu)
MX (2) MX340242B (hu)
PL (5) PL2634242T3 (hu)
PT (5) PT2634242T (hu)
SI (5) SI2634243T1 (hu)
TW (3) TWI627274B (hu)
WO (1) WO2008006494A1 (hu)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2557046A1 (en) 2004-03-05 2005-10-13 John Crowley Process for cell culturing by continuous perfusion and alternating tangential flow
US8585594B2 (en) * 2006-05-24 2013-11-19 Phoenix Biomedical, Inc. Methods of assessing inner surfaces of body lumens or organs
KR101685246B1 (ko) 2006-07-14 2016-12-09 디피엑스 홀딩스 비.브이. 개선된 세포 배양 방법
US9109193B2 (en) 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
US8623650B2 (en) 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum
US9238792B2 (en) * 2009-09-15 2016-01-19 E I Du Pont De Nemours And Company Compartmentalized simultaneous saccharification and fermentation of biomass
CA2777116C (en) 2009-10-15 2020-09-01 Crucell Holland B.V. Process for adenovirus purification from high cell density cultures
JP5393896B2 (ja) 2009-10-15 2014-01-22 クルセル ホランド ベー ヴェー アデノウイルス粒子の精製方法
WO2012030512A1 (en) 2010-09-03 2012-03-08 Percivia Llc. Flow-through protein purification process
EP2957628A1 (en) * 2010-10-05 2015-12-23 Novo Nordisk Health Care AG Process for protein production
SG192611A1 (en) 2011-02-10 2013-09-30 Crucell Holland Bv Pneumatic alternating pressure membrane cell separation system
KR101591671B1 (ko) * 2011-04-29 2016-02-04 바이오콘 리서치 리미티드 항체의 이질성을 감소시키는 방법 및 그 항체의 제조방법
ES2970057T3 (es) 2011-05-13 2024-05-24 Octapharma Ag Un procedimiento para aumentar la productividad de células eucarióticas en la producción de FVIII recombinante
PT2837680T (pt) 2011-07-01 2020-04-17 Amgen Inc Cultura celular de mamífero
TWI637057B (zh) 2012-11-09 2018-10-01 拜爾沙納有限公司 具交替生物反應器用途之不連續進料批次製程
JP6017060B2 (ja) * 2012-11-29 2016-10-26 イー・エム・デイー・ミリポア・コーポレイシヨン フード付き磁気インペラアセンブリを備える容器
HUE056073T2 (hu) * 2013-03-15 2022-01-28 Genzyme Corp Magas denzitású sejtbanki tárolási eljárások
ES2842350T3 (es) 2013-03-19 2021-07-13 Cmc Biologics As Un procedimiento para la producción de un producto (por ejemplo, polipéptido) en un proceso de fermentación de cultivo celular continuo
BR112016005545B1 (pt) * 2013-09-16 2021-06-01 Genzyme Corporation Método de processamento de uma cultura de células de mamífero
BR112016006063A2 (pt) * 2013-09-30 2017-08-01 Crucell Holland Bv método para clarificação de uma colheita de cultura celular em bruto
AU2015269365A1 (en) 2014-06-04 2016-12-15 Amgen Inc. Methods for harvesting mammalian cell cultures
CN106414719B (zh) * 2014-06-13 2020-02-14 杰特有限公司 生物反应器中重组von Willebrand因子的改进生产
EP3172317B1 (en) 2014-07-24 2019-05-01 Janssen Vaccines & Prevention B.V. Process for the purification of poliovirus from cell cultures
JP6530171B2 (ja) * 2014-09-09 2019-06-12 旭化成メディカル株式会社 培養産生物の回収方法
US11674952B2 (en) 2016-02-24 2023-06-13 The Rockefeller University Embryonic cell-based therapeutic candidate screening systems, models for Huntington's Disease and uses thereof
US10358626B2 (en) 2016-04-12 2019-07-23 Artemis Biosystems, Inc. Perfusion filtration systems
CN110268043A (zh) * 2017-03-03 2019-09-20 富士胶片株式会社 细胞培养装置及细胞培养方法
US11609120B2 (en) 2017-10-06 2023-03-21 Lonza Ltd Automated control of cell culture using Raman spectroscopy
EP4039786A1 (en) 2017-10-16 2022-08-10 Regeneron Pharmaceuticals, Inc. Perfusion bioreactor and related methods of use
EP3700505A4 (en) 2017-10-26 2021-01-06 Repligen Corporation MICRO-ALTERNATIVE TANGENTIAL FLOW INFUSION FILTER, PROCESSING CONTAINER, AND METHODS OF USE THEREOF
AU2018383679B2 (en) * 2017-12-11 2025-10-09 Amgen Inc. Continuous manufacturing process for bispecific antibody products
SG11202005782UA (en) 2018-03-08 2020-09-29 Repligen Corp Tangential flow depth filtration systems and methods of filtration using same
EP3770266A4 (en) * 2018-03-19 2021-06-09 FUJIFILM Corporation PRODUCT MANUFACTURING PROCESS
KR20200141455A (ko) 2018-05-25 2020-12-18 리플리겐 코포레이션 접선 유동 여과 시스템 및 방법
CN109157690A (zh) * 2018-06-15 2019-01-08 翁炳焕 猴-人细胞融合母胎血型不合治疗杂交株的制备
JP7707062B2 (ja) * 2018-11-02 2025-07-14 ウーシー バイオロジクス アイルランド リミテッド 連続的採取を行い細胞流出を行わない強化灌流による細胞培養法
EP3647405A1 (en) * 2018-11-02 2020-05-06 Microfluidx Cell culture device and method of using the same
CN113924355B (zh) * 2019-05-28 2024-04-02 上海药明生物技术有限公司 用于监测和自动控制灌流细胞培养的拉曼光谱集成灌流细胞培养系统
US12460170B2 (en) 2019-11-07 2025-11-04 Merck Patent Gmbh Methods and systems for performing perfusion cell culture
WO2021183687A2 (en) 2020-03-10 2021-09-16 Cellares Corporation Systems, devices, and methods for cell processing
US12252678B2 (en) 2021-12-01 2025-03-18 Microfluidx Ltd Systems and methods for bioprocessing
WO2024228717A2 (en) 2022-09-07 2024-11-07 Quantitative Biosciences, Inc. Fentanyl-specific single variable-domain antibodies and use in a continuous agglutination assay
US11898127B1 (en) 2022-09-26 2024-02-13 Upside Foods, Inc. Harvesting cell-based meat
WO2024072444A1 (en) * 2022-09-26 2024-04-04 Upside Foods, Inc. Harvesting cell-based meat
US12180453B2 (en) 2023-03-21 2024-12-31 Cellares Corporation Systems, devices, and methods for electroporation within a cell processing system
WO2024226439A1 (en) 2023-04-24 2024-10-31 Quantitative Biosciences, Inc. Engineered biosensor strains of e. coli for continuous aerobic detection of analytes
US12399193B2 (en) 2023-05-05 2025-08-26 Cellares Corporation Systems, devices, and methods for combined cell processes
WO2025007051A2 (en) 2023-06-30 2025-01-02 Cellares Corporation Systems, devices, and methods for fluid transfer within an automated cell processing system
US12497587B2 (en) 2023-08-21 2025-12-16 Cellares Corporation Bioreactors and methods of their use in automatic cell processing systems
US12305156B2 (en) 2023-08-21 2025-05-20 Cellares Corporation Systems, devices, and methods for fluid control in a cell processing system
WO2025041064A2 (en) 2023-08-21 2025-02-27 Cellares Corporation Systems, devices, and methods for automatic cell sorting
WO2025128343A1 (en) 2023-12-11 2025-06-19 Just-Evotec Biologics, Inc. Protein expression using trans-splicing and split selectable markers
US12492368B2 (en) 2024-03-11 2025-12-09 Cellares Corporation Monitoring air pressure within a cell processing system
WO2025202944A2 (en) 2024-03-27 2025-10-02 Cellares Corporation Liquid level and flow rate detection within a cell processing system
USD1109621S1 (en) 2024-04-15 2026-01-20 Cellares Corporation Analytical platform for biological material testing
EP4711442A1 (en) 2024-09-15 2026-03-18 Quantitative Biosciences, Inc. Microfluidic chip for the parallel culture of microbial biosensor strains

Family Cites Families (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4477342A (en) 1982-08-31 1984-10-16 Becton, Dickinson And Company Apparatus and method for controlling ultrafiltration during hemodialysis
EP0205404B1 (en) * 1985-06-11 1992-07-15 Ciba-Geigy Ag Hybrid interferons
US5286646A (en) * 1985-11-25 1994-02-15 Boehringer Mannheim Gmbh Method for mammalian cell culture
PT84991B (pt) 1986-06-06 1990-03-08 Genentech Inc Processo para a producao de actividador do plasminogenio biologicamente activo
JPH0642905B2 (ja) 1986-06-13 1994-06-08 東レ株式会社 血液透析膜
GB8617646D0 (en) 1986-07-18 1986-08-28 Manchester Inst Science Tech Recovery of biological material
US6022742A (en) * 1986-11-26 2000-02-08 Kopf; Henry B. Culture device and method
US4806484A (en) * 1987-08-07 1989-02-21 Igb Products, Ltd. Perfusion airlift bioreactor
JPH0797982B2 (ja) 1987-09-24 1995-10-25 東洋紡績株式会社 細胞培養装置
US4921792A (en) 1987-11-27 1990-05-01 Miles Inc. Continuous cell dispersion, cultivation and substance recovery process
JPH01243985A (ja) 1988-03-25 1989-09-28 P C C Technol:Kk 植物器官の培養法及びその培養槽
US5595909A (en) 1988-05-23 1997-01-21 Regents Of The University Of Minnesota Filter device
JPH02119772A (ja) * 1988-10-29 1990-05-07 Shimadzu Corp 細胞培養装置
JP2625990B2 (ja) * 1988-11-19 1997-07-02 株式会社島津製作所 細胞培養装置
US5019512A (en) 1989-03-17 1991-05-28 Baxter International Inc. Spin filter for removing substantially cell-free culture medium from suspension cell culture system
JP2690144B2 (ja) * 1989-04-24 1997-12-10 哲哉 峠 膜型細胞培養装置
JPH0793874B2 (ja) * 1989-04-27 1995-10-11 日本碍子株式会社 バイオリアクタ
US5256294A (en) 1990-09-17 1993-10-26 Genentech, Inc. Tangential flow filtration process and apparatus
JP2948675B2 (ja) * 1991-03-20 1999-09-13 日本碍子株式会社 発酵液中の有価物を回収するクロスフロー濾過方法
JPH0531332A (ja) * 1991-07-30 1993-02-09 Toto Ltd 微生物の膜分離方法
GB9118664D0 (en) 1991-08-30 1991-10-16 Celltech Ltd Cell culture
JPH07123972A (ja) * 1993-11-01 1995-05-16 Fumio Yamauchi 膜型バイオリアクター
JPH07298871A (ja) * 1994-05-06 1995-11-14 Fumio Yamauchi 膜型バイオリアクター
US6204000B1 (en) 1995-04-28 2001-03-20 The United States Of America As Represented By The Department Of Health And Human Services Diagnostic methods and gene therapy using reagents derived from the human metastasis suppressor gene KAI1
IL160406A0 (en) 1995-06-15 2004-07-25 Crucell Holland Bv A cell harbouring nucleic acid encoding adenoritus e1a and e1b gene products
US5994728A (en) 1995-11-15 1999-11-30 Matsushita Electronics Corporation Field effect transistor and method for producing the same
JPH1015357A (ja) * 1996-07-01 1998-01-20 Nitto Denko Corp 排水の処理方法
JPH1033671A (ja) * 1996-07-24 1998-02-10 Jms Co Ltd 中空糸型人工肝臓とその灌流及び培養システム
JP3346996B2 (ja) 1996-09-18 2002-11-18 金剛株式会社 免震自立棚
JPH10108673A (ja) * 1996-10-04 1998-04-28 Asahi Medical Co Ltd 中空糸型培養器を用いた動物細胞の培養方法
JPH10108666A (ja) * 1996-10-04 1998-04-28 Asahi Medical Co Ltd 中空糸型培養器
US6001585A (en) 1997-11-14 1999-12-14 Cellex Biosciences, Inc. Micro hollow fiber bioreactor
US8026096B1 (en) 1998-10-08 2011-09-27 Protein Sciences Corporation In vivo active erythropoietin produced in insect cells
DE60041322D1 (de) 1999-01-26 2009-02-26 Schering Plough Ltd Vermehrung des bovinen coronavirus in cho (chinese hamster ovary) zellen
US6855544B1 (en) 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
HK1048138B (zh) 1999-04-15 2009-06-12 荷兰克鲁塞尔公司 用编码腺病毒e1蛋白的序列在人体细胞中生产重组蛋白
JP2001149099A (ja) 1999-11-26 2001-06-05 Olympus Optical Co Ltd 染色体異常解析のための有核細胞の標本作製方法
EP1103610A1 (en) 1999-11-26 2001-05-30 Introgene B.V. Production of vaccines from immortalised mammalian cell lines
US6544424B1 (en) 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
US6168941B1 (en) 2000-04-07 2001-01-02 Genvec, Inc. Method of producing adenoviral vector stocks
JP4138209B2 (ja) 2000-06-29 2008-08-27 エーザイ・アール・アンド・ディー・マネジメント株式会社 プラバスタチン含有組成物
JP2002113896A (ja) 2000-10-05 2002-04-16 Seiko Epson Corp 画像形成装置
EP1363857A2 (en) 2001-02-23 2003-11-26 V.A.I. Ltd. Methods and apparatus for biological treatment of waste waters
JP3547715B2 (ja) * 2001-03-06 2004-07-28 榮 新垣 ベクター精製用装置及び方法
US6716354B2 (en) 2001-03-08 2004-04-06 Cdg Technology, Inc. Methods of treating water using combinations of chlorine dioxide, chlorine and ammonia
WO2002086133A1 (fr) 2001-04-17 2002-10-31 Seiren Kabushiki Kaisha Additifs de milieu et milieux de culture de cellules animales
DE10120835C2 (de) 2001-04-27 2003-04-10 Sifin Inst Fuer Immunpraeparat Verfahren zur Herstellung von monoklonalen Antikörpern
DE10120838A1 (de) 2001-04-27 2002-10-31 Basf Ag Stoffmischung zur UV-Stabilisierung von Kunststoffen und Herstellung davon
DK1451290T3 (da) 2001-08-31 2011-04-26 Bayer Schering Pharma Ag Indretning og fremgangsmåde til udførelse af fermentering af høj celledensitet
DE60207824T2 (de) 2001-09-05 2006-07-06 Yamasa Corp., Chosi Cytidin-diphosphocholin gegen diabetische neuropathie
US20030054544A1 (en) 2001-09-14 2003-03-20 Medcell Biologics, Inc. Oxygen enriched bioreactor and method of culturing cells
GB0123098D0 (en) * 2001-09-26 2001-11-14 Lonza Biologics Plc Use of aminoglycoside resistance gene
ES2243794T3 (es) 2001-11-15 2005-12-01 AESCULAP AG &amp; CO. KG Recipiente esteril.
JP2003219873A (ja) 2002-01-24 2003-08-05 Japan Science & Technology Corp サケ科キングサーモン由来の不死化細胞
US20030186335A1 (en) 2002-03-28 2003-10-02 National Institute Of Advanced Industrial Science And Technology Monoclonal antibodies, hybridomas, cell isolation method, isolated cells and immunological diagnostic method
US6902909B2 (en) 2002-07-09 2005-06-07 Synthecon, Inc. Methods for efficient production of mammalian recombinant proteins
DE10237082B4 (de) 2002-08-09 2014-12-31 Sartorius Stedim Biotech Gmbh Verfahren und Vorrichtung zur biotechnologischen Herstellung von Wertstoffen
US20070065907A1 (en) * 2003-05-01 2007-03-22 Dms Ip Assets B.V. Process for the production of biological substances by perfusion culturing of suspended animal cells
WO2004099396A1 (en) * 2003-05-09 2004-11-18 Crucell Holland B.V. Cultures of e1-immortalized cells and processes for culturing the same to increase product yields therefrom
CA2532754A1 (en) * 2003-07-17 2005-02-03 Global Cell Solutions, Llc Automated cell culture system and process
JP2005041984A (ja) 2003-07-22 2005-02-17 Shin Kobe Electric Mach Co Ltd 水架橋ポリオレフィン製シートの製造法
CA2557046A1 (en) 2004-03-05 2005-10-13 John Crowley Process for cell culturing by continuous perfusion and alternating tangential flow
WO2005094963A1 (en) * 2004-03-29 2005-10-13 Pall Corporation Pleated, crossflow fluid treatment elements
EP1835022B1 (en) * 2005-01-05 2015-02-11 Chugai Seiyaku Kabushiki Kaisha Cell culture method and utilization of the same
CN100354408C (zh) * 2005-10-09 2007-12-12 南京工业大学 一种高密度细胞培养方法及其生物反应装置
EP1968740B8 (en) * 2005-12-17 2017-02-22 AirInSpace S.E. Air purification devices
JP5031332B2 (ja) 2006-03-31 2012-09-19 旭化成ホームズ株式会社 線切断具
KR101685246B1 (ko) 2006-07-14 2016-12-09 디피엑스 홀딩스 비.브이. 개선된 세포 배양 방법
JP2008308560A (ja) 2007-06-13 2008-12-25 Tohcello Co Ltd 表面防汚性複合フィルムの製造方法
EP2014760A1 (en) 2007-06-13 2009-01-14 CMC Biopharmaceuticals A/S A method for producing a biopolymer (e.g. polypeptide) in a continuous fermentation process
JP5426937B2 (ja) * 2009-06-19 2014-02-26 興和株式会社 光学的反応測定装置および光学的反応測定方法

Also Published As

Publication number Publication date
US8685731B2 (en) 2014-04-01
AU2007272054A1 (en) 2008-01-17
EP2634250B1 (en) 2017-04-05
EP2041259B2 (en) 2024-10-09
PL2634250T3 (pl) 2017-09-29
EP2041259B1 (en) 2015-10-14
EP2634242B1 (en) 2017-04-05
TWI512102B (zh) 2015-12-11
EP2634243B2 (en) 2024-07-24
JP2014217395A (ja) 2014-11-20
DK2634243T3 (en) 2017-12-11
ES2625066T3 (es) 2017-07-18
WO2008006494A1 (en) 2008-01-17
EP2634242A1 (en) 2013-09-04
DK2634243T4 (da) 2024-09-23
BRPI0714347A2 (pt) 2013-05-07
DK2343362T4 (da) 2024-07-08
TW201341527A (zh) 2013-10-16
PT2634243T (pt) 2017-12-18
MX2009000522A (es) 2009-01-27
AR062072A1 (es) 2008-10-15
PL2343362T3 (pl) 2016-11-30
CN104263700A (zh) 2015-01-07
SI2634250T1 (sl) 2017-06-30
PT2634242T (pt) 2017-07-12
KR20130014640A (ko) 2013-02-07
PT2343362T (pt) 2016-07-13
US20140154745A1 (en) 2014-06-05
US20100075413A1 (en) 2010-03-25
ES2579957T3 (es) 2016-08-17
DK2343362T3 (en) 2016-07-18
HUE028365T2 (hu) 2016-12-28
EP2041259A1 (en) 2009-04-01
AU2007272054C1 (en) 2014-08-28
ES2650042T3 (es) 2018-01-16
EP2343362B1 (en) 2016-03-30
TW200813216A (en) 2008-03-16
EA201101581A1 (ru) 2012-04-30
EP2343362A1 (en) 2011-07-13
SI2041259T1 (sl) 2016-04-29
EP2634243B1 (en) 2017-09-06
EP2634243A1 (en) 2013-09-04
HUE028593T2 (hu) 2016-12-28
PL2634243T3 (pl) 2018-02-28
US9469865B2 (en) 2016-10-18
US20130095527A1 (en) 2013-04-18
SI2634242T1 (sl) 2017-07-31
EA016451B1 (ru) 2012-05-30
US8222001B2 (en) 2012-07-17
SI2343362T1 (sl) 2016-09-30
PT2634250T (pt) 2017-07-13
ES2559308T3 (es) 2016-02-11
FI2343362T4 (fi) 2024-07-04
JP2011067222A (ja) 2011-04-07
DK2041259T3 (en) 2016-01-25
CL2007002060A1 (es) 2008-04-18
AU2007272054B2 (en) 2011-09-08
EP2634250A1 (en) 2013-09-04
HUE034299T2 (hu) 2018-02-28
TWI413689B (zh) 2013-11-01
US8440458B2 (en) 2013-05-14
JP5448010B2 (ja) 2014-03-19
IL234890A (en) 2016-05-31
HUE035109T2 (hu) 2018-05-02
KR20090031899A (ko) 2009-03-30
SI2634243T1 (en) 2018-01-31
CA2657040A1 (en) 2008-01-17
JP2012165764A (ja) 2012-09-06
US20160265017A1 (en) 2016-09-15
PL2634242T3 (pl) 2017-09-29
JP5092129B2 (ja) 2012-12-05
JP2009543565A (ja) 2009-12-10
ES2625062T3 (es) 2017-07-18
PL2343362T5 (pl) 2024-12-02
US20120149107A1 (en) 2012-06-14
PT2041259E (pt) 2016-02-15
TW201604277A (zh) 2016-02-01
EP2343362B8 (en) 2016-05-18
KR20120009530A (ko) 2012-01-31
MX340242B (es) 2016-07-01
US8119368B2 (en) 2012-02-21
US9670520B2 (en) 2017-06-06
DK2634242T3 (en) 2017-05-22
TWI627274B (zh) 2018-06-21
US20120149063A1 (en) 2012-06-14
CA2657040C (en) 2013-10-01
ES2579957T5 (es) 2024-12-10
EA200900188A1 (ru) 2009-06-30
PL2041259T3 (pl) 2016-04-29
IL196322A0 (en) 2009-09-22
KR101685246B1 (ko) 2016-12-09
KR101143796B1 (ko) 2012-05-21
EP2343362B2 (en) 2024-04-03
CN102876628A (zh) 2013-01-16
US20120064623A1 (en) 2012-03-15

Similar Documents

Publication Publication Date Title
US9670520B2 (en) Process for the culturing of cells
AU2013204016B2 (en) Improved process for the culturing of cells
HK1189024B (en) Improved process for the culturing of cells
HK1189025B (en) Improved process for the culturing of cells
HK1189034B (en) Improved process for the culturing of cells
HK1189024A (en) Improved process for the culturing of cells
HK1189025A (en) Improved process for the culturing of cells
HK1189034A (en) Improved process for the culturing of cells