IL108607A - Preparation of attached photoproteins and method of using them - Google Patents
Preparation of attached photoproteins and method of using themInfo
- Publication number
- IL108607A IL108607A IL10860794A IL10860794A IL108607A IL 108607 A IL108607 A IL 108607A IL 10860794 A IL10860794 A IL 10860794A IL 10860794 A IL10860794 A IL 10860794A IL 108607 A IL108607 A IL 108607A
- Authority
- IL
- Israel
- Prior art keywords
- photoprotein
- sulfhydryl
- binding reagent
- aequorin
- binding
- Prior art date
Links
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- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 1
- 229960005147 calcium acetate Drugs 0.000 description 1
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- 239000001639 calcium acetate Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
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- 238000004440 column chromatography Methods 0.000 description 1
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- 108010031180 cypridina luciferase Proteins 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000001641 gel filtration chromatography Methods 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 108700014210 glycosyltransferase activity proteins Proteins 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000012500 ion exchange media Substances 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000008176 lyophilized powder Substances 0.000 description 1
- 125000005439 maleimidyl group Chemical group C1(C=CC(N1*)=O)=O 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 102000035118 modified proteins Human genes 0.000 description 1
- 108091005573 modified proteins Proteins 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 150000003230 pyrimidines Chemical group 0.000 description 1
- 238000006862 quantum yield reaction Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- VUFNRPJNRFOTGK-UHFFFAOYSA-M sodium;1-[4-[(2,5-dioxopyrrol-1-yl)methyl]cyclohexanecarbonyl]oxy-2,5-dioxopyrrolidine-3-sulfonate Chemical compound [Na+].O=C1C(S(=O)(=O)[O-])CC(=O)N1OC(=O)C1CCC(CN2C(C=CC2=O)=O)CC1 VUFNRPJNRFOTGK-UHFFFAOYSA-M 0.000 description 1
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- 125000006850 spacer group Chemical group 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1711693A | 1993-02-12 | 1993-02-12 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL108607A0 IL108607A0 (en) | 1994-05-30 |
| IL108607A true IL108607A (en) | 1998-12-06 |
Family
ID=21780817
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL10860794A IL108607A (en) | 1993-02-12 | 1994-02-09 | Preparation of attached photoproteins and method of using them |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5486455A (de) |
| EP (1) | EP0683822B1 (de) |
| JP (1) | JPH08506897A (de) |
| AT (1) | ATE217086T1 (de) |
| AU (1) | AU6171894A (de) |
| DE (1) | DE69430523T2 (de) |
| IL (1) | IL108607A (de) |
| MX (1) | MX9401112A (de) |
| WO (1) | WO1994018342A1 (de) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4438660A1 (de) * | 1994-10-28 | 1996-05-02 | Boehringer Mannheim Gmbh | Biolumineszenzmarkierte Hapten-Konjugate für den Einsatz in kompetitiven Immunoassays |
| EP0736770A3 (de) * | 1995-04-05 | 1997-05-02 | Anda Biolog Sa | Immunoreaktives Konjugat, Verfahren zu seiner Herstellung, gegen dieses gerichtete Antikörper und sie enthaltende pharmazeutische Zusammensetzung und diagnostische Vorrichtung |
| DE19537952A1 (de) | 1995-10-12 | 1997-04-17 | Boehringer Mannheim Gmbh | Verfahren zum Nachweis eines Analyten |
| US5876995A (en) * | 1996-02-06 | 1999-03-02 | Bryan; Bruce | Bioluminescent novelty items |
| US5942407A (en) * | 1996-06-25 | 1999-08-24 | Immunomatrix, Inc. | Light-emitting immunoassay |
| US6416960B1 (en) | 1996-08-08 | 2002-07-09 | Prolume, Ltd. | Detection and visualization of neoplastic tissues and other tissues |
| IL129767A0 (en) | 1996-12-12 | 2000-02-29 | Prolume Ltd | Apparatus and method for detecting and identifying infectious agents |
| DE19652576A1 (de) * | 1996-12-17 | 1998-06-25 | Boehringer Mannheim Gmbh | Niedermolekulare Photoprotein-Konjugate |
| US5876935A (en) * | 1997-01-08 | 1999-03-02 | Dade Behring Inc. | Luminescent specific binding assay |
| WO1998036081A2 (en) | 1997-02-13 | 1998-08-20 | Memorial Sloan-Kettering Cancer Center | Hybrid molecules for optically detecting changes in cellular microenvironments |
| AU6658898A (en) * | 1997-02-18 | 1998-09-08 | Oncor, Inc. | Detection of targets with green fluorescent protein and fluorescent variants thereof |
| DE19719465A1 (de) * | 1997-05-07 | 1998-11-12 | Boehringer Mannheim Gmbh | Konservierung von Aequorin-Reagenzien |
| EP1925320A3 (de) | 1998-03-27 | 2008-09-03 | Prolume, Ltd. | Luciferasen, fluoreszierende Proteine, Nukleinsäuren zur Kodierung der Luciferasen und der fluoreszierenden Proteine sowie deren Verwendung zur Diagnose, zum Screening mit hohem Durchsatz und zur Behandlung neuartiger Probleme |
| JP2002507410A (ja) | 1998-03-27 | 2002-03-12 | プロルーム・リミテッド | ルシフェラーゼ、蛍光タンパク質、ルシフェラーゼおよび蛍光タンパク質をコードする核酸および、診断、高処理スクリーニングおよび新規アイテムにおけるその使用 |
| JP2003502670A (ja) * | 1999-06-18 | 2003-01-21 | カージオジェニックス， インコーポレイテッド | 化学発光結合アッセイを実施するための方法 |
| US7109315B2 (en) | 2000-03-15 | 2006-09-19 | Bruce J. Bryan | Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items |
| US7659078B1 (en) * | 2002-07-17 | 2010-02-09 | University Of Kentucky Research Foundation | Method and kit for determination of prostacyclin in plasma |
| US20080280310A1 (en) * | 2007-05-09 | 2008-11-13 | Louis Panagopoulos | Testing for Blood Group Immunological Reaction Without the Use of Anti-Human Globulin |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4604364A (en) * | 1974-01-04 | 1986-08-05 | Kosak Kenneth M | Bioluminescent tracer composition and method of use in immunoassays |
| GB1578275A (en) * | 1977-06-14 | 1980-11-05 | Maier C | Procedure for the assay of pharmacologically immunologically and biochemically active compounds in biological fluids |
| US4396579A (en) * | 1981-08-06 | 1983-08-02 | Miles Laboratories, Inc. | Luminescence detection device |
| US4608246A (en) * | 1983-03-10 | 1986-08-26 | Immucor, Inc. | Testing for a blood group immunological reaction |
| DK487784A (da) * | 1983-10-13 | 1985-04-14 | Univ Georgia | Immunoassay |
| US4665022A (en) * | 1984-02-17 | 1987-05-12 | The Regents Of The University Of California | Bioluminescent assay reagent and method |
| US4927916A (en) * | 1984-04-23 | 1990-05-22 | The General Hospital Corporation | Method of producing fibrin-specific monoclonal antibodies lacking fibrinogen-cross-reactivity using fibrin-specific peptides |
| US4764368A (en) * | 1984-08-29 | 1988-08-16 | Dana-Farber Cancer Institute, Inc. | Acid-cleavable compound |
| US4657853A (en) * | 1984-09-14 | 1987-04-14 | E. I. Du Pont De Nemours And Company | Immunoassays utilizing covalent conjugates of polymerized enzyme and antibody |
| US4935339A (en) * | 1985-05-07 | 1990-06-19 | Nichols Institute Diagnostics | Delayed solid phase immunologic assay |
| DE3537877A1 (de) * | 1985-10-24 | 1987-04-30 | Geiger Reinhard | Luciferin-derivate und immunoassays unter einsatz derartiger luciferin-derivate |
| GB8603537D0 (en) * | 1986-02-13 | 1986-03-19 | Parker D | Conjugate compound |
| US4916070A (en) * | 1986-04-14 | 1990-04-10 | The General Hospital Corporation | Fibrin-specific antibodies and method of screening for the antibodies |
| DE3714147A1 (de) * | 1987-04-28 | 1988-11-17 | Boehringer Mannheim Gmbh | Immunchemisches verfahren und reagenz zur bestimmung eines polyvalenten antigens in einer fluessigen probe |
| JPS6447379A (en) * | 1987-08-19 | 1989-02-21 | Chisso Corp | Preparation of calcium-dependent oxygenation enzyme |
| US4981979A (en) * | 1987-09-10 | 1991-01-01 | Neorx Corporation | Immunoconjugates joined by thioether bonds having reduced toxicity and improved selectivity |
| US5094939A (en) * | 1988-07-19 | 1992-03-10 | Fujirebio, Inc. | Chemiluminescence assays using stabilized dioxetane derivatives |
| US4925648A (en) * | 1988-07-29 | 1990-05-15 | Immunomedics, Inc. | Detection and treatment of infectious and inflammatory lesions |
| US5112615A (en) * | 1988-08-03 | 1992-05-12 | New England Deaconess Hospital Corporation | Soluble hirudin conjugates |
| US5019393A (en) * | 1988-08-03 | 1991-05-28 | New England Deaconess Hospital Corporation | Biocompatible substance with thromboresistance |
| US5135736A (en) * | 1988-08-15 | 1992-08-04 | Neorx Corporation | Covalently-linked complexes and methods for enhanced cytotoxicity and imaging |
| US5066789A (en) * | 1988-09-30 | 1991-11-19 | Neorx Corporation | Targeting substance-diagnostic/therapeutic agent conjugates having Schiff base linkages |
| JPH02253162A (ja) * | 1989-03-27 | 1990-10-11 | Chisso Corp | 特異的結合能を有する物質と結合したエクオリンを用いる検出法 |
| WO1990006774A1 (en) * | 1988-12-22 | 1990-06-28 | Xoma Corporation | Hindered linking agents and methods |
| US5134071A (en) * | 1989-02-06 | 1992-07-28 | State University Of New York | Polymerization and copolymerization of proteins |
| JPH03153699A (ja) * | 1989-11-09 | 1991-07-01 | Chisso Corp | 化学結合法により担体に固定化されたエクオリン活性を示す蛋白質 |
| JPH03169897A (ja) * | 1989-11-29 | 1991-07-23 | Chisso Corp | 化学修飾エクオリンとその調製法 |
| US5116944A (en) * | 1989-12-29 | 1992-05-26 | Neorx Corporation | Conjugates having improved characteristics for in vivo administration |
-
1994
- 1994-02-04 WO PCT/US1994/001387 patent/WO1994018342A1/en not_active Ceased
- 1994-02-04 JP JP6518303A patent/JPH08506897A/ja active Pending
- 1994-02-04 AT AT94908734T patent/ATE217086T1/de active
- 1994-02-04 DE DE69430523T patent/DE69430523T2/de not_active Expired - Fee Related
- 1994-02-04 AU AU61718/94A patent/AU6171894A/en not_active Abandoned
- 1994-02-04 EP EP94908734A patent/EP0683822B1/de not_active Expired - Lifetime
- 1994-02-09 IL IL10860794A patent/IL108607A/en not_active IP Right Cessation
- 1994-02-11 MX MX9401112A patent/MX9401112A/es unknown
- 1994-08-22 US US08/293,648 patent/US5486455A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE69430523T2 (de) | 2002-11-28 |
| AU6171894A (en) | 1994-08-29 |
| ATE217086T1 (de) | 2002-05-15 |
| IL108607A0 (en) | 1994-05-30 |
| EP0683822B1 (de) | 2002-05-02 |
| EP0683822A1 (de) | 1995-11-29 |
| JPH08506897A (ja) | 1996-07-23 |
| EP0683822A4 (de) | 1997-11-05 |
| DE69430523D1 (de) | 2002-06-06 |
| MX9401112A (es) | 1994-08-31 |
| US5486455A (en) | 1996-01-23 |
| WO1994018342A1 (en) | 1994-08-18 |
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Legal Events
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| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed | ||
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| KB | Patent renewed | ||
| MM9K | Patent not in force due to non-payment of renewal fees |