IL79760A - Purification and activation of proteins from insoluble inclusion bodies - Google Patents

Purification and activation of proteins from insoluble inclusion bodies

Info

Publication number: IL79760A
Authority: IL; Israel
Prior art keywords: protein; inclusion bodies; solution; purification; sds
Prior art date: 1985-08-19

Application number

IL79760A

Other languages

English (en)

Other versions

IL79760A0 (en

Original Assignee

Int Minerals & Chem Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-08-19

Filing date

1986-08-18

Publication date

1992-02-16

1986-08-18 Application filed by Int Minerals & Chem Corp filed Critical Int Minerals & Chem Corp

1986-11-30 Publication of IL79760A0 publication Critical patent/IL79760A0/xx

1992-02-16 Publication of IL79760A publication Critical patent/IL79760A/xx

Links

102000004169 proteins and genes Human genes 0.000 title abstract 6
108090000623 proteins and genes Proteins 0.000 title abstract 6
210000003000 inclusion body Anatomy 0.000 title abstract 3
238000000746 purification Methods 0.000 title abstract 2
230000004913 activation Effects 0.000 title 1
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 abstract 3
238000005349 anion exchange Methods 0.000 abstract 3
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 2
239000004202 carbamide Substances 0.000 abstract 2
238000004587 chromatography analysis Methods 0.000 abstract 2
239000000243 solution Substances 0.000 abstract 2
239000008366 buffered solution Substances 0.000 abstract 1
244000005700 microbiome Species 0.000 abstract 1
239000012460 protein solution Substances 0.000 abstract 1
230000007928 solubilization Effects 0.000 abstract 1
238000005063 solubilization Methods 0.000 abstract 1
238000000108 ultra-filtration Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/82—Proteins from microorganisms
- Y10S530/825—Bacteria

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Endocrinology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Medicinal Chemistry (AREA)
Crystallography & Structural Chemistry (AREA)
Toxicology (AREA)
Zoology (AREA)
Gastroenterology & Hepatology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Analytical Chemistry (AREA)
Peptides Or Proteins (AREA)
Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Treatment Of Liquids With Adsorbents In General (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Detergent Compositions (AREA)

IL79760A 1985-08-19 1986-08-18 Purification and activation of proteins from insoluble inclusion bodies IL79760A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US06/767,032 US4766224A (en)	1985-08-19	1985-08-19	Purification and activation of proteins from insoluble inclusion bodies

Publications (2)

Publication Number	Publication Date
IL79760A0 IL79760A0 (en)	1986-11-30
IL79760A true IL79760A (en)	1992-02-16

Family

ID=25078290

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL79760A IL79760A (en)	1985-08-19	1986-08-18	Purification and activation of proteins from insoluble inclusion bodies

Country Status (13)

Country	Link
US (1)	US4766224A (de)
EP (1)	EP0212960B1 (de)
AT (1)	ATE91285T1 (de)
BR (1)	BR8603944A (de)
CA (1)	CA1267495C (de)
DE (1)	DE3688663T2 (de)
DK (1)	DK394786A (de)
ES (1)	ES2000287A6 (de)
IE (1)	IE61756B1 (de)
IL (1)	IL79760A (de)
MX (1)	MX168137B (de)
PT (1)	PT83205B (de)
ZA (1)	ZA866250B (de)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB8508340D0 (en) *	1985-03-29	1985-05-09	Creighton T E	Production of protein
US4705848A (en) *	1986-06-02	1987-11-10	International Minerals & Chemical Corp.	Isolation of bioactive, monomeric growth hormone
AU612133B2 (en) *	1987-02-20	1991-07-04	Natinco Nv	Production of proteins in active forms
US5013653A (en)	1987-03-20	1991-05-07	Creative Biomolecules, Inc.	Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
CA1339757C (en) *	1987-04-16	1998-03-17	Robert F. Halenbeck	Production of purified biologically active, bacterially produced recombinant human csf-1
US4929700A (en) *	1987-04-16	1990-05-29	Cetus Corporation	Production of purified, biologically active, bacterially produced recombinant human CSF-1
CA1305285C (en) *	1987-04-21	1992-07-14	Malcolm Roy Brandon	Production of proteins in active forms
US4931543A (en) *	1987-05-11	1990-06-05	Cetus Corporation	Process for recovering microbially produced interleukin-2
US5162507A (en) *	1987-05-11	1992-11-10	Cetus Corporation	Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4961969A (en) *	1987-05-11	1990-10-09	Cetus Corporation	Process for recovering microbially produced interferon-β
US4801691A (en) *	1987-05-15	1989-01-31	International Minerals & Chemical Corp.	Method for removing sodium dodecyl sulfate from sodium dodecyl sulfate solubilized protein solutions
US6384194B1 (en)	1987-12-16	2002-05-07	Dsm N.V.	Expression and purification of human interleukin-3 and muteins thereof
EP0325691B1 (de) *	1988-01-25	1993-04-07	Consortium für elektrochemische Industrie GmbH	Verfahren zur Solubilisierung von unlöslichem Protein
US4981952A (en) *	1988-10-04	1991-01-01	Eli Lilly And Company	Method for the purification of vitamin K-dependent proteins
US5136027A (en) *	1989-05-02	1992-08-04	Abbott Laboratories	Method for renaturing proteins in the presence of alkyl sulfate detergents
US5179196A (en) *	1989-05-04	1993-01-12	Sri International	Purification of proteins employing ctap-iii fusions
US4975529A (en) *	1989-08-18	1990-12-04	Monsanto Company	Method of folding somatotropins
US5530100A (en) *	1990-05-07	1996-06-25	Rhone-Poulenc Rorer Pharmaceuticals Inc.	Methods for purification of recombinantly produced proteins
US5023323A (en) *	1990-09-12	1991-06-11	Monsanto Company	Method of somatotropin naturation
US5605691A (en) *	1992-09-17	1997-02-25	Ophidian Pharmaceuticals, Inc.	Immunologically active proteins from inclusion bodies
AU4727296A (en) *	1995-02-24	1996-09-11	Cantab Pharmaceuticals Research Limited	Polypeptides useful as immunotherapeutic agents and methods of polypeptide preparation
CA2223745C (en) *	1995-06-07	2004-03-16	Chiron Corporation	Method of solubilizing, purifying, and refolding protein
KR100374306B1 (ko) *	1995-07-14	2003-07-12	주식회사 엘지생명과학	재조합효모로부터과립구-콜로니자극인자를정제하는방법
ZA9711733B (en) *	1996-12-31	1998-07-01	Monsanto Co	Method for solubilization and naturation of somatotropins
JP2001512306A (ja) *	1997-01-08	2001-08-21	ライフテクノロジーズ，インコーポレイテッド	タンパク質の産生方法
ATE464123T1 (de) *	1997-06-20	2010-04-15	Univ New York	Elektrosprühen von lösungen zur massenherstellung von chips und molekülbibliotheken
US6068993A (en) *	1997-07-02	2000-05-30	Biobras Sa	Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
ATE349460T1 (de) *	1998-07-09	2007-01-15	Barofold Inc	Rückfaltung von protein-aggregaten und einschlussteilchen durch hohen druck
CA2434045A1 (en) *	2000-10-31	2002-08-15	The Regents Of The University Of Colorado, A Body Corporate	Improved protein disaggregation and refolding using high pressure
WO2008042495A2 (en)	2006-07-21	2008-04-10	Life Technologies Corporation	Sharply resolving labeled protein molecular weight standards
CA2663416A1 (en) *	2006-09-15	2008-03-20	Barofold, Inc.	High pressure treatment of proteins for reduced immunogenicity
EP2291388A4 (de) *	2008-06-03	2013-02-13	Patrys Ltd	Verfahren zur aufreinigung von antikörpern
EP2475677B1 (de) *	2009-08-26	2018-08-01	Commonwealth Scientific and Industrial Research Organisation	Verfahren zur herstellung einer seidendotierung
CN102977183B (zh) *	2012-12-11	2016-08-17	鲁南新时代生物技术有限公司	一种基因工程包涵体的洗涤方法
CN113773371B (zh) *	2021-09-16	2024-06-18	北京凯悦宁医药科技有限公司	一种vp1蛋白及其制备方法和应用
KR102781939B1 (ko) *	2021-11-01	2025-03-13	전남대학교산학협력단	바이러스 구조 단백질의 생산방법

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4426323A (en) *	1980-01-10	1984-01-17	Ionics, Incorporated	Selected recovery of proteins from fermentation broths
US4462940A (en) *	1982-09-23	1984-07-31	Cetus Corporation	Process for the recovery of human β-interferon-like polypeptides
US4511503A (en) *	1982-12-22	1985-04-16	Genentech, Inc.	Purification and activity assurance of precipitated heterologous proteins
US4599197A (en) *	1982-12-22	1986-07-08	Genentech, Inc.	Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) *	1982-12-22	1985-04-16	Genentech, Inc.	Purification and activity assurance of precipitated heterologous proteins
IE56372B1 (en) *	1982-12-22	1991-07-03	Genentech Inc	Microbially produced rennet methods for its production and plasmids used for its production
GR79124B (de) *	1982-12-22	1984-10-02	Genentech Inc
US4518526A (en) *	1982-12-22	1985-05-21	Genentech, Inc.	Purification and activity assurance of precipitated heterologous proteins
US4677196A (en) *	1985-09-06	1987-06-30	International Minerals & Chemical Corp.	Purification and activation of proteins from insoluble inclusion bodies

1985
- 1985-08-19 US US06/767,032 patent/US4766224A/en not_active Expired - Lifetime
1986
- 1986-08-14 PT PT83205A patent/PT83205B/pt not_active IP Right Cessation
- 1986-08-14 CA CA515964A patent/CA1267495C/en not_active Expired
- 1986-08-18 DE DE86306353T patent/DE3688663T2/de not_active Expired - Fee Related
- 1986-08-18 EP EP86306353A patent/EP0212960B1/de not_active Expired - Lifetime
- 1986-08-18 IL IL79760A patent/IL79760A/xx not_active IP Right Cessation
- 1986-08-18 IE IE221186A patent/IE61756B1/en not_active IP Right Cessation
- 1986-08-18 BR BR8603944A patent/BR8603944A/pt not_active IP Right Cessation
- 1986-08-18 AT AT86306353T patent/ATE91285T1/de active
- 1986-08-19 ZA ZA866250A patent/ZA866250B/xx unknown
- 1986-08-19 DK DK394786A patent/DK394786A/da not_active Application Discontinuation
- 1986-08-19 ES ES8601195A patent/ES2000287A6/es not_active Expired
- 1986-08-19 MX MX003479A patent/MX168137B/es unknown

Also Published As

Publication number	Publication date
ZA866250B (en)	1987-03-25
IL79760A0 (en)	1986-11-30
BR8603944A (pt)	1987-03-24
DK394786D0 (da)	1986-08-19
IE61756B1 (en)	1994-11-30
US4766224A (en)	1988-08-23
PT83205A (en)	1986-09-01
CA1267495A (en)	1990-04-03
PT83205B (pt)	1988-07-01
EP0212960A3 (en)	1989-02-22
EP0212960A2 (de)	1987-03-04
DE3688663D1 (de)	1993-08-12
CA1267495C (en)	1990-04-03
ATE91285T1 (de)	1993-07-15
DK394786A (da)	1987-02-20
MX168137B (es)	1993-05-06
IE862211L (en)	1987-02-19
EP0212960B1 (de)	1993-07-07
ES2000287A6 (es)	1988-02-01
DE3688663T2 (de)	1993-10-28

Legal Events

Date	Code	Title
1996-10-31	FF	Patent granted
2001-01-11	KB	Patent renewed
2005-07-25	MM9K	Patent not in force due to non-payment of renewal fees

Publication	Publication Date	Title
ZA866250B (en)	1987-03-25	Purification and activation of proteins from insoluble inclusion bodies
DE3684078D1 (de)	1992-04-09	Reinigung und aktivierung von proteinen aus unloeslichen einschlusskoerpern.
PT80172B (pt)	1987-06-17	Processo para recuperar e purificar o recombinante interleuquina-2
EP0215625A3 (en)	1989-04-12	Protein recovery
AU569896B2 (en)	1988-02-25	Process for the dissolution of peptides and proteins
ATE86627T1 (de)	1993-03-15	Isolierung von bioaktivem, monomerem wachstumshormon.
GB1401737A (en)	1975-07-30	Anti-coagulant isolation
ATE113062T1 (de)	1994-11-15	Throminbindende substanz und verfahren zu ihrer herstellung.
GB1419929A (en)	1975-12-31	Method for the preparation of high purity kallikrein
IE38688L (en)	1974-01-19	Purification of orgotein
Rausch et al.	1987	Purification and activation of proteins from insoluble inclusion bodies.
JPS6438097A (en)	1989-02-08	Method for reactivating protein
KR960031478A (ko)	1996-09-17	유비퀴틴 융합 인간 칼시토닌의 정제방법
HUP9903078A2 (hu)	1999-12-28	Eljárás rekombináns, nem-lipidezett Osp-protein előállítására és tisztítására
KR910016926A (ko)	1991-11-05	인간 β-인터페론의 정제방법
GB1440998A (en)	1976-06-30	Tuberculin active proteins and peptides