IL81937A - Modified human tissue-type plasminogen activator devoid of functional carbohydrate structure at amino acid residue 117 having increased in vivo half-life, its preparation and pharmaceutical composition containing said molecule - Google Patents

Modified human tissue-type plasminogen activator devoid of functional carbohydrate structure at amino acid residue 117 having increased in vivo half-life, its preparation and pharmaceutical composition containing said molecule

Info

Publication number: IL81937A
Authority: IL; Israel
Prior art keywords: human tissue; plasminogen activator; type plasminogen; amino acid; type
Prior art date: 1986-03-18

Application number

IL8193787A

Other languages

English (en)

Other versions

IL81937A0 (en

Original Assignee

Genentech Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-03-18

Filing date

1987-03-18

Publication date

1994-07-31

1987-03-18 Application filed by Genentech Inc filed Critical Genentech Inc

1987-10-20 Publication of IL81937A0 publication Critical patent/IL81937A0/xx

1994-07-31 Publication of IL81937A publication Critical patent/IL81937A/en

Links

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125000000837 carbohydrate group Chemical group 0.000 title claims abstract 5
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238000001727 in vivo Methods 0.000 title claims description 10
239000008194 pharmaceutical composition Substances 0.000 title claims description 4
238000002360 preparation method Methods 0.000 title abstract description 4
238000000034 method Methods 0.000 claims description 25
108020004414 DNA Proteins 0.000 claims description 19
108020004705 Codon Proteins 0.000 claims description 17
235000001014 amino acid Nutrition 0.000 claims description 16
229940024606 amino acid Drugs 0.000 claims description 15
150000001413 amino acids Chemical class 0.000 claims description 15
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WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 7
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims description 6
230000004071 biological effect Effects 0.000 claims description 6
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125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 claims 1
125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 claims 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

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Bioinformatics & Cheminformatics (AREA)
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General Health & Medical Sciences (AREA)
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Diabetes (AREA)
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General Chemical & Material Sciences (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Pharmacology & Pharmacy (AREA)
Microbiology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Peptides Or Proteins (AREA)

IL8193787A 1986-03-18 1987-03-18 Modified human tissue-type plasminogen activator devoid of functional carbohydrate structure at amino acid residue 117 having increased in vivo half-life, its preparation and pharmaceutical composition containing said molecule IL81937A (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US84107586A	1986-03-18	1986-03-18
US2189387A	1987-03-04	1987-03-04

Publications (2)

Publication Number	Publication Date
IL81937A0 IL81937A0 (en)	1987-10-20
IL81937A true IL81937A (en)	1994-07-31

Family

ID=26695219

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL8193787A IL81937A (en)	1986-03-18	1987-03-18	Modified human tissue-type plasminogen activator devoid of functional carbohydrate structure at amino acid residue 117 having increased in vivo half-life, its preparation and pharmaceutical composition containing said molecule

Country Status (19)

Country	Link
EP (1)	EP0238304B1 (de)
JP (1)	JP2972812B2 (de)
AT (1)	ATE139569T1 (de)
AU (1)	AU602510B2 (de)
CA (1)	CA1341597C (de)
DD (1)	DD258999A5 (de)
DE (2)	DE3708681C2 (de)
DK (1)	DK175490B1 (de)
ES (1)	ES2088860T3 (de)
FI (1)	FI100802B (de)
FR (1)	FR2597883B1 (de)
GB (1)	GB2189249B (de)
GR (1)	GR3020850T3 (de)
HU (1)	HU202276B (de)
IE (1)	IE81073B1 (de)
IL (1)	IL81937A (de)
NO (1)	NO172133C (de)
NZ (1)	NZ219654A (de)
PT (1)	PT84493B (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0178105B1 (de) *	1984-10-01	1997-04-02	Genzyme Corporation	Rekombinante DNS-Verfahren und dadurch hergestellte Produkte
US5108909A (en) *	1985-03-22	1992-04-28	Chiron Corporation	Expression of TPA in mammalian cells
ES2031826T3 (es) *	1985-12-23	1993-01-01	Chiron Corporation	Procedimiento para producir nuevos peptidos activadores del plasminogeno.
WO1987004722A1 (en) *	1986-01-31	1987-08-13	Genetics Institute, Inc.	Novel thrombolytic proteins
US4851517A (en) *	1986-02-26	1989-07-25	Monsanto Company	Tissue plasminogen activator oligosaccharide from normal human colon cells
US4927630A (en) *	1986-02-26	1990-05-22	Monsanto Company	Tissue plasminogen activator from normal human colon cells
JPH084501B2 (ja) *	1987-03-20	1996-01-24	エーザイ株式会社	糖鎖に関する変異ｔＰＡ
EP0293934B1 (de) *	1987-06-04	1994-08-31	Zymogenetics, Inc.	Mutierter t-PA mit Kringle-Ersatz
AU615919B2 (en) *	1987-07-01	1991-10-17	Beecham Group Plc	Hybrid plasminogen activators
US5244676A (en) *	1987-10-09	1993-09-14	Monsanto Company	Modified tissue plasminogen activator with modified glycosylation site
IL88247A0 (en) *	1987-11-06	1989-06-30	Genentech Inc	Novel human tissue-type plasminogen activator variant
US5346824A (en) *	1988-05-20	1994-09-13	Genentech, Inc.	DNA encoding variants of tissue plasminogen activators and expression vectors and hosts thereof
IL90146A (en) *	1988-05-20	1994-10-07	Genentech Inc	Glycosylation derivatives of tissue plasminogen activator
US5270198A (en) *	1988-05-20	1993-12-14	Genentech, Inc.	DNA molecules encoding variants of tissue plasminogen activators, vectors, and host cells
US5714145A (en) *	1988-09-02	1998-02-03	Genentech, Inc.	Tissue plasminogen activator having zymogenic or fibrin specific properties
US5108901A (en) *	1988-09-02	1992-04-28	Genentech, Inc.	Tissue plasminogen activator having zymogenic or fibrin specific properties
US5262170A (en) *	1988-09-02	1993-11-16	Genentech, Inc.	Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
DE3841296A1 (de) *	1988-12-08	1990-06-13	Bosch Gmbh Robert	Antiblockierregelsystem
US5070021A (en) *	1989-01-10	1991-12-03	Monsanto Company	Method of modifying oligosaccharide structure of tissue plasminogen activator
US5242688A (en) *	1990-12-24	1993-09-07	Eli Lilly And Company	Method of treating thromboembolic disorders by administration of diglycosylated t-pa variants
US5223408A (en) *	1991-07-11	1993-06-29	Genentech, Inc.	Method for making variant secreted proteins with altered properties
ATE155816T1 (de)	1992-06-03	1997-08-15	Genentech Inc	Varianten des gewebeplasminogenaktivators mit verbesserter therapeutischer wirkung
US6706504B1 (en)	1996-11-12	2004-03-16	The Scripps Research Institute	Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
US6506598B1 (en)	1999-04-26	2003-01-14	Genentech, Inc.	Cell culture process
US7084118B2 (en)	2002-02-22	2006-08-01	Genentech, Inc.	Combination treatment with t-PA variant and low molecular weight heparin

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
HU200615B (en) *	1982-05-05	1990-07-28	Genentech Inc	Methods of preparing tissue plasiminogen activator derivative composition
EP0125254A1 (de) *	1982-10-28	1984-11-21	Beecham Group Plc	Enzymderivate und deren verwendung bei der behandlung von trombosen
EP0125269A1 (de) *	1982-11-11	1984-11-21	Beecham Group Plc	Pharmazeutisch aktive verbindungen
EP0178105B1 (de) *	1984-10-01	1997-04-02	Genzyme Corporation	Rekombinante DNS-Verfahren und dadurch hergestellte Produkte
DE3537176C2 (de) *	1984-10-18	1994-06-09	Zymogenetics Inc	Gewebeplasminogenaktivator und Verfahren zu seiner Herstellung
JPS61247384A (ja) *	1984-10-18	1986-11-04	チモ−ジエネテイツクス　インコ−ポレ−テツド	酵母中でプラスミノ−ゲン活性化因子を発現する方法
GR861037B (en) *	1985-04-22	1986-07-16	Genentech Inc	Novel human tissue plasminogen activator mutants
JPH0811066B2 (ja) *	1985-09-30	1996-02-07	インテグレーテッド・ジエネティックス・インコーポレーテッド	組換えｄｎａが生産するヒト子宮プラスミノ−ゲンアクチベ−タ−
GB8528321D0 (en) *	1985-11-18	1985-12-24	Ciba Geigy Ag	Modified fibrinolytic agents
ES2031826T3 (es) *	1985-12-23	1993-01-01	Chiron Corporation	Procedimiento para producir nuevos peptidos activadores del plasminogeno.
WO1987004722A1 (en) *	1986-01-31	1987-08-13	Genetics Institute, Inc.	Novel thrombolytic proteins

1987
- 1987-03-16 IE IE69187A patent/IE81073B1/en not_active IP Right Cessation
- 1987-03-17 ES ES87302267T patent/ES2088860T3/es not_active Expired - Lifetime
- 1987-03-17 NZ NZ219654A patent/NZ219654A/xx unknown
- 1987-03-17 NO NO871086A patent/NO172133C/no unknown
- 1987-03-17 GB GB8706249A patent/GB2189249B/en not_active Expired - Lifetime
- 1987-03-17 PT PT84493A patent/PT84493B/pt unknown
- 1987-03-17 FR FR878703644A patent/FR2597883B1/fr not_active Expired - Lifetime
- 1987-03-17 AT AT87302267T patent/ATE139569T1/de not_active IP Right Cessation
- 1987-03-17 HU HU871152A patent/HU202276B/hu unknown
- 1987-03-17 AU AU70088/87A patent/AU602510B2/en not_active Expired
- 1987-03-17 DK DK198701358A patent/DK175490B1/da not_active IP Right Cessation
- 1987-03-17 DE DE3708681A patent/DE3708681C2/de not_active Expired - Lifetime
- 1987-03-17 DE DE3751842T patent/DE3751842T2/de not_active Expired - Lifetime
- 1987-03-17 EP EP87302267A patent/EP0238304B1/de not_active Expired - Lifetime
- 1987-03-18 IL IL8193787A patent/IL81937A/en not_active IP Right Cessation
- 1987-03-18 DD DD30090087A patent/DD258999A5/de unknown
- 1987-03-18 FI FI871175A patent/FI100802B/fi not_active IP Right Cessation
- 1987-03-18 JP JP62065536A patent/JP2972812B2/ja not_active Expired - Lifetime
- 1987-03-18 CA CA000532353A patent/CA1341597C/en not_active Expired - Fee Related
1996
- 1996-08-22 GR GR960402207T patent/GR3020850T3/el unknown

Also Published As

Publication number	Publication date
PT84493A (en)	1987-04-01
NO172133C (no)	1993-06-09
AU602510B2 (en)	1990-10-18
DD258999A5 (de)	1988-08-10
GB2189249B (en)	1990-10-31
JP2972812B2 (ja)	1999-11-08
FI871175L (fi)	1987-09-19
NO871086D0 (no)	1987-03-17
GR3020850T3 (en)	1996-11-30
IL81937A0 (en)	1987-10-20
NO172133B (no)	1993-03-01
NO871086L (no)	1987-09-21
GB2189249A (en)	1987-10-21
JPS62282582A (ja)	1987-12-08
DK135887D0 (da)	1987-03-17
ATE139569T1 (de)	1996-07-15
EP0238304A2 (de)	1987-09-23
IE870691L (en)	1987-09-18
DE3751842D1 (de)	1996-07-25
PT84493B (pt)	1989-11-10
AU7008887A (en)	1987-09-24
HUT43108A (en)	1987-09-28
FR2597883A1 (fr)	1987-10-30
EP0238304B1 (de)	1996-06-19
CA1341597C (en)	2009-06-23
ES2088860T3 (es)	1996-10-01
FI100802B (fi)	1998-02-27
IE81073B1 (en)	2000-01-12
NZ219654A (en)	1990-08-28
FR2597883B1 (fr)	1990-04-20
DK175490B1 (da)	2004-11-08
HU202276B (en)	1991-02-28
EP0238304A3 (en)	1988-07-06
DE3708681A1 (de)	1987-10-01
DK135887A (da)	1987-09-19
DE3751842T2 (de)	1996-11-28
GB8706249D0 (en)	1987-04-23
FI871175A0 (fi)	1987-03-18
DE3708681C2 (de)	1995-08-17

Legal Events

Date	Code	Title
1997-06-10	KB	Patent renewed
2001-06-14	KB	Patent renewed
2005-05-17	KB	Patent renewed
2007-07-24	EXP	Patent expired

Publication	Publication Date	Title
CA1341597C (en)	2009-06-23	Mutant human tissue-type plasminogen activator with a modified carbohydrate structure at amino acid residue 117
US5308617A (en)	1994-05-03	Protein heparin conjugates
US4853330A (en)	1989-08-01	Human tissue plasminogen activator
Lau et al.	1987	A Modified Human Tissue Plasminogen Activator with Extended Half–Life In Vivo
Cole et al.	1993	In vivo clearance of tissue plasminogen activator: the complex role of sites of glycosylation and level of sialylation
AU624158B2 (en)	1992-06-04	Variants of plasminogen activators and processes for their production
EP0297066B1 (de)	1993-08-18	Neue fibrinolytische Enzyme
CA1341609C (en)	2011-02-01	Novel human tissue-type plasminogen activator variant
US5589361A (en)	1996-12-31	Human tissue-type plasminogen activator variant
HK1009289A (en)	1999-05-28	Novel human tissue-type plasminogen activator variant
IL104953A (en)	2002-08-14	Method for producing tissue-type plasminogen activator by transforming and cultivating a host cell incapable of forming complex glycoproteins
Li et al.	1992	Biochemical properties of recombinant mutants of nonglycosylated single chain urokinase-type plasminogen activator
US6682733B1 (en)	2004-01-27	Fibrinolytic enzymes
WO1989007641A1 (en)	1989-08-24	Enhancement of the therapeutic properties of glycoprotein