JP4636876B2 - ポリペプチドの高収率生産のための方法およびｄｎａ構築物 - Google Patents

ポリペプチドの高収率生産のための方法およびｄｎａ構築物 Download PDF

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JP4636876B2
JP4636876B2 JP2004508263A JP2004508263A JP4636876B2 JP 4636876 B2 JP4636876 B2 JP 4636876B2 JP 2004508263 A JP2004508263 A JP 2004508263A JP 2004508263 A JP2004508263 A JP 2004508263A JP 4636876 B2 JP4636876 B2 JP 4636876B2
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polypeptide
amino acid
protease
expression cassette
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JP2006508639A (ja
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ジェイムズ・エイ・ウィリアムズ
ルアン・ペン
ユアナン・シャ
スコット・ハーレー
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Medtronic Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/635—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Endocrinology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2004508263A 2002-05-24 2003-05-23 ポリペプチドの高収率生産のための方法およびｄｎａ構築物 Expired - Fee Related JP4636876B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38337002P 2002-05-24 2002-05-24
PCT/US2003/016643 WO2003100021A2 (en) 2002-05-24 2003-05-23 Methods and dna constructs for high yield production of polypeptides

Related Child Applications (1)

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JP2010181250A Division JP5455840B2 (ja) 2002-05-24 2010-08-13 ポリペプチドの高収率生産のための方法およびｄｎａ構築物

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JP2006508639A JP2006508639A (ja) 2006-03-16
JP4636876B2 true JP4636876B2 (ja) 2011-02-23

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JP2004508263A Expired - Fee Related JP4636876B2 (ja) 2002-05-24 2003-05-23 ポリペプチドの高収率生産のための方法およびｄｎａ構築物
JP2010181250A Expired - Fee Related JP5455840B2 (ja) 2002-05-24 2010-08-13 ポリペプチドの高収率生産のための方法およびｄｎａ構築物

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US (3) US7501484B2 (de)
EP (1) EP1532261B1 (de)
JP (2) JP4636876B2 (de)
AT (1) ATE457355T1 (de)
AU (1) AU2003239895C1 (de)
CA (1) CA2485835C (de)
DE (1) DE60331226D1 (de)
DK (1) DK1532261T3 (de)
ES (1) ES2343061T3 (de)
WO (1) WO2003100021A2 (de)

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EP1411968B1 (de) * 2001-07-31 2008-09-17 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Glp 1 exendin 4 peptidanaloga und deren verwendungen
AU2003243316A1 (en) * 2002-05-24 2003-12-12 Nps Allelix Corp. Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides
ATE457355T1 (de) * 2002-05-24 2010-02-15 Medtronic Inc Verfahren und dna-konstrukte zur produktion von polypeptiden mit hoher ausbeute
WO2003100022A2 (en) * 2002-05-24 2003-12-04 Restoragen, Inc. Methods and dna constructs for high yield production of polypeptides
EP1539959A2 (de) * 2002-09-18 2005-06-15 Centre Hospitalier de l'Université de Montréal (CHUM) Ghrh-analoge
EP1529844B2 (de) † 2003-07-21 2018-11-07 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Komplexbildung zur Stabilisierung und Reinigung eines Proteins von Interesse
US7829307B2 (en) * 2003-11-21 2010-11-09 Nps Pharmaceuticals, Inc. Production of glucagon-like peptide 2
US20060069021A1 (en) * 2004-08-13 2006-03-30 Nastech Pharmaceutical Company Inc. Compositions and methods for intranasal administration of inactive analogs of PTH or inactivated preparations of PTH or PTH analogs
GB0426160D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Production of proteins
GB0426161D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Protein isolation and purification
CN101595228A (zh) 2005-07-21 2009-12-02 艾博特公司 包括sorf构建体的多基因表达和使用多蛋白、前体蛋白和蛋白酶解的方法
US7736633B2 (en) 2005-09-28 2010-06-15 E.I. Du Pont De Nemours And Company Method for enhancing effects of colorants and conditioners
US20100234568A1 (en) * 2006-10-19 2010-09-16 Linda Jane Decarolis Identification of peptide tags for the production of insoluble peptides by sequence scanning
US7662913B2 (en) 2006-10-19 2010-02-16 E. I. Du Pont De Nemours And Company Cystatin-based peptide tags for the expression and purification of bioactive peptides
US7732569B2 (en) 2006-10-19 2010-06-08 E.I. Du Pont De Nemours And Company Zein-based peptide tags for the expression and purification of bioactive peptides
US7951559B2 (en) * 2007-07-25 2011-05-31 E.I. Du Pont De Nemours And Company Recombinant peptide production using a cross-linkable solubility tag
US7829311B2 (en) 2007-07-25 2010-11-09 E.I. Du Pont De Nemours And Company Ketosteroid isomerase inclusion body tag engineered to be acid-resistant by replacing aspartates with glutamate
US7678883B2 (en) * 2007-07-25 2010-03-16 E.I. Du Pont De Nemours And Company Solubility tags for the expression and purification of bioactive peptides
WO2010047347A1 (ja) * 2008-10-21 2010-04-29 財団法人化学及血清療法研究所 封入体形成タンパク質の製造方法
US9062312B2 (en) 2011-06-21 2015-06-23 Danisco Us Inc. Fusion peptides comprising multi-functional peptidic solubility tags for efficient production, processing and surface applications
SG11201405721VA (en) * 2012-03-19 2014-10-30 Madeleine Pharmaceuticals Ρτy Ltd Method of producing a recombinant peptide
EP2792686A1 (de) 2013-04-17 2014-10-22 Heinrich-Heine-Universität Düsseldorf Verfahren zur Expression von Peptiden und Proteinen
JP6548063B2 (ja) * 2014-09-11 2019-07-24 国立大学法人 岡山大学 タンパク質、融合タンパク質の製造法及び精製法
RU2562869C1 (ru) * 2014-10-31 2015-09-10 Федеральное государственное бюджетное учреждение науки Институт биохимии им. А.Н. Баха Российской академии наук (ИНБИ РАН) ИНТЕГРАТИВНАЯ ГЕНЕТИЧЕСКАЯ КОНСТРУКЦИЯ pQ-SRUS ДЛЯ ПОЛУЧЕНИЯ ШТАММОВ ДРОЖЖЕЙ Yarrowia lipolytica, ОБЛАДАЮЩИХ СПОСОБНОСТЬЮ К ГОМОЛОГИЧНОЙ РЕКОМБИНАЦИИ ГЕНОМА МИТОХОНДРИЙ ЗА СЧЁТ ЭКСПРЕССИИ ГЕНА БЕЛКА RecA БАКТЕРИАЛЬНОГО ПРОИСХОЖДЕНИЯ
DK3242685T3 (da) 2015-01-09 2022-07-25 Adalta Ltd Cxcr4-bindende molekyler
CN108640972B (zh) * 2018-05-17 2021-07-30 中国药科大学 与vista具有亲和力的短肽及其用途
CN110656119B (zh) * 2019-10-09 2021-05-25 武汉博欧特生物科技有限公司 一种用于观察细菌蛋白定位的表达载体及其构建方法
AU2022247419A1 (en) 2021-03-31 2023-10-05 Biological E Limited Constructs and methods for increased expression of polypeptides
EP4444739A2 (de) 2021-12-08 2024-10-16 Oakgrove Bio LLC Biologische herstellung von histidinreichen peptiden
WO2024010489A2 (en) * 2022-07-08 2024-01-11 Shemyakin-Ovchinnikov Institute Of Bioorganic Chemistry Of The Russian Academy Of Sciences New hybrid protein cbd-hs-es-glp1, recombinant plasmid for its production, escherichia coli producer strain and method of producing glp-1 polypeptide
WO2024261777A1 (en) * 2023-06-19 2024-12-26 Biological E Limited Expression cassettes for protein expression, method(s) and application(s) thereof

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GB9203803D0 (en) * 1992-02-21 1992-04-08 Wellcome Found A recombinant polypeptide
US5814603A (en) * 1992-06-12 1998-09-29 Affymax Technologies N.V. Compounds with PTH activity
US5352771A (en) * 1992-08-31 1994-10-04 Iowa State University Research Foundation Inc. Hydrolysis of peptide bonds using Pt (II) and Pd (II) complexes
JP3802051B2 (ja) * 1993-08-16 2006-07-26 リサーチ ディベロップメント ファンデーション 膵臓島細胞におけるインシュリン表現を刺激する新しいホメオボックス因子
US5747453A (en) * 1995-06-06 1998-05-05 Alza Corporation Method for increasing the electrotransport flux of polypeptides
JP2001512306A (ja) * 1997-01-08 2001-08-21 ライフ テクノロジーズ，インコーポレイテッド タンパク質の産生方法
WO1998052966A1 (en) * 1997-05-19 1998-11-26 The Johns Hopkins University School Of Medecine Tissue specific prodrug
SI1724284T1 (sl) * 2000-12-07 2009-12-31 Lilly Co Eli Glp-1 fuzijski proteini
ATE457355T1 (de) * 2002-05-24 2010-02-15 Medtronic Inc Verfahren und dna-konstrukte zur produktion von polypeptiden mit hoher ausbeute

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Publication number Publication date
US20170029860A1 (en) 2017-02-02
EP1532261A2 (de) 2005-05-25
WO2003100021A3 (en) 2004-09-10
US20090170192A1 (en) 2009-07-02
DK1532261T3 (da) 2010-05-31
AU2003239895A1 (en) 2003-12-12
CA2485835C (en) 2018-03-06
US9951368B2 (en) 2018-04-24
US9322025B2 (en) 2016-04-26
DE60331226D1 (de) 2010-03-25
AU2003239895B2 (en) 2009-06-04
JP2006508639A (ja) 2006-03-16
ATE457355T1 (de) 2010-02-15
CA2485835A1 (en) 2003-12-04
JP2011004757A (ja) 2011-01-13
EP1532261B1 (de) 2010-02-10
JP5455840B2 (ja) 2014-03-26
WO2003100021A2 (en) 2003-12-04
US7501484B2 (en) 2009-03-10
AU2003239895C1 (en) 2010-01-07
US20050221444A1 (en) 2005-10-06
ES2343061T3 (es) 2010-07-22
EP1532261A4 (de) 2006-08-23

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