JPH0142670B2 - - Google Patents

Info

Publication number: JPH0142670B2
Authority: JP; Japan
Prior art keywords: peroxidase; culture; medium; callus; plant
Prior art date: 1982-08-09
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP13869882A

Other languages

English (en)

Japanese (ja)

Other versions

JPS5928473A (ja

Inventor

Setsuo Kobayashi

Teruo Oomori

Ryotaro Kotani

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Toyobo Co Ltd

Original Assignee

Toyobo Co Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1982-08-09

Filing date

1982-08-09

Publication date

1989-09-13

1982-08-09 Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd

1982-08-09 Priority to JP13869882A priority Critical patent/JPS5928473A/ja

1984-02-15 Publication of JPS5928473A publication Critical patent/JPS5928473A/ja

1989-09-13 Publication of JPH0142670B2 publication Critical patent/JPH0142670B2/ja

Status Granted legal-status Critical Current

Links

102000003992 Peroxidases Human genes 0.000 claims description 38
108040007629 peroxidase activity proteins Proteins 0.000 claims description 38
241000196324 Embryophyta Species 0.000 claims description 21
240000003291 Armoracia rusticana Species 0.000 claims description 8
235000011330 Armoracia rusticana Nutrition 0.000 claims description 8
238000012258 culturing Methods 0.000 claims description 7
238000004519 manufacturing process Methods 0.000 claims description 5
235000015097 nutrients Nutrition 0.000 claims description 4
206010020649 Hyperkeratosis Diseases 0.000 description 17
239000002609 medium Substances 0.000 description 16
241000220259 Raphanus Species 0.000 description 12
244000195452 Wasabia japonica Species 0.000 description 12
235000000760 Wasabia japonica Nutrition 0.000 description 12
235000006140 Raphanus sativus var sativus Nutrition 0.000 description 11
239000000243 solution Substances 0.000 description 11
102000004190 Enzymes Human genes 0.000 description 10
108090000790 Enzymes Proteins 0.000 description 10
238000000034 method Methods 0.000 description 9
239000001963 growth medium Substances 0.000 description 6
230000000694 effects Effects 0.000 description 5
238000004161 plant tissue culture Methods 0.000 description 5
229930192334 Auxin Natural products 0.000 description 4
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
229910052921 ammonium sulfate Inorganic materials 0.000 description 4
235000011130 ammonium sulphate Nutrition 0.000 description 4
239000002363 auxin Substances 0.000 description 4
239000000463 material Substances 0.000 description 4
239000000203 mixture Substances 0.000 description 4
239000000126 substance Substances 0.000 description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 3
229920005654 Sephadex Polymers 0.000 description 3
239000012507 Sephadex™ Substances 0.000 description 3
239000007864 aqueous solution Substances 0.000 description 3
239000004062 cytokinin Substances 0.000 description 3
UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 3
238000003306 harvesting Methods 0.000 description 3
QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 3
229960001669 kinetin Drugs 0.000 description 3
102000004169 proteins and genes Human genes 0.000 description 3
108090000623 proteins and genes Proteins 0.000 description 3
239000007787 solid Substances 0.000 description 3
239000006228 supernatant Substances 0.000 description 3
238000012360 testing method Methods 0.000 description 3
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
108010044467 Isoenzymes Proteins 0.000 description 2
244000046052 Phaseolus vulgaris Species 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
239000000872 buffer Substances 0.000 description 2
229910052799 carbon Inorganic materials 0.000 description 2
230000004663 cell proliferation Effects 0.000 description 2
239000012153 distilled water Substances 0.000 description 2
238000005194 fractionation Methods 0.000 description 2
SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 2
239000004615 ingredient Substances 0.000 description 2
239000007788 liquid Substances 0.000 description 2
230000007935 neutral effect Effects 0.000 description 2
239000008363 phosphate buffer Substances 0.000 description 2
238000005185 salting out Methods 0.000 description 2
239000002689 soil Substances 0.000 description 2
SODPIMGUZLOIPE-UHFFFAOYSA-N (4-chlorophenoxy)acetic acid Chemical compound OC(=O)COC1=CC=C(Cl)C=C1 SODPIMGUZLOIPE-UHFFFAOYSA-N 0.000 description 1
239000005971 1-naphthylacetic acid Substances 0.000 description 1
239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 1
CDAWCLOXVUBKRW-UHFFFAOYSA-N 2-aminophenol Chemical compound NC1=CC=CC=C1O CDAWCLOXVUBKRW-UHFFFAOYSA-N 0.000 description 1
IIDAJRNSZSFFCB-UHFFFAOYSA-N 4-amino-5-methoxy-2-methylbenzenesulfonamide Chemical compound COC1=CC(S(N)(=O)=O)=C(C)C=C1N IIDAJRNSZSFFCB-UHFFFAOYSA-N 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
241000219196 Armoracia Species 0.000 description 1
235000011293 Brassica napus Nutrition 0.000 description 1
240000008100 Brassica rapa Species 0.000 description 1
235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
240000003421 Dianthus chinensis Species 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 1
244000017020 Ipomoea batatas Species 0.000 description 1
235000002678 Ipomoea batatas Nutrition 0.000 description 1
NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
244000061176 Nicotiana tabacum Species 0.000 description 1
235000002637 Nicotiana tabacum Nutrition 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
235000021307 Triticum Nutrition 0.000 description 1
244000098338 Triticum aestivum Species 0.000 description 1
239000002253 acid Substances 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
239000008272 agar Substances 0.000 description 1
150000001298 alcohols Chemical class 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
238000003149 assay kit Methods 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
230000032823 cell division Effects 0.000 description 1
239000012295 chemical reaction liquid Substances 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
238000003795 desorption Methods 0.000 description 1
238000007865 diluting Methods 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
239000004744 fabric Substances 0.000 description 1
239000003337 fertilizer Substances 0.000 description 1
238000002523 gelfiltration Methods 0.000 description 1
239000008103 glucose Substances 0.000 description 1
235000021332 kidney beans Nutrition 0.000 description 1
239000012533 medium component Substances 0.000 description 1
239000008267 milk Substances 0.000 description 1
235000013336 milk Nutrition 0.000 description 1
210000004080 milk Anatomy 0.000 description 1
239000004570 mortar (masonry) Substances 0.000 description 1
229930014626 natural product Natural products 0.000 description 1
150000007524 organic acids Chemical class 0.000 description 1
235000005985 organic acids Nutrition 0.000 description 1
239000003375 plant hormone Substances 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
230000002062 proliferating effect Effects 0.000 description 1
238000011403 purification operation Methods 0.000 description 1
239000000700 radioactive tracer Substances 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
239000007974 sodium acetate buffer Substances 0.000 description 1
SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
230000001954 sterilising effect Effects 0.000 description 1
238000004659 sterilization and disinfection Methods 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
229930003231 vitamin Natural products 0.000 description 1
235000013343 vitamin Nutrition 0.000 description 1
239000011782 vitamin Substances 0.000 description 1
229940088594 vitamin Drugs 0.000 description 1
238000005406 washing Methods 0.000 description 1
229940023877 zeatin Drugs 0.000 description 1

Landscapes

Enzymes And Modification Thereof (AREA)

JP13869882A 1982-08-09 1982-08-09 ペルオキシダ−ゼの製造法 Granted JPS5928473A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP13869882A JPS5928473A (ja)	1982-08-09	1982-08-09	ペルオキシダ−ゼの製造法

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP13869882A JPS5928473A (ja)	1982-08-09	1982-08-09	ペルオキシダ−ゼの製造法

Publications (2)

Publication Number	Publication Date
JPS5928473A JPS5928473A (ja)	1984-02-15
JPH0142670B2 true JPH0142670B2 (it)	1989-09-13

Family

ID=15228029

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP13869882A Granted JPS5928473A (ja)	1982-08-09	1982-08-09	ペルオキシダ−ゼの製造法

Country Status (1)

Country	Link
JP (1)	JPS5928473A (it)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS62172667A (ja) *	1986-01-23	1987-07-29	Japan Storage Battery Co Ltd	蓄電池の充電方法
JP2534043B2 (ja) *	1986-10-01	1996-09-11	金印わさび株式会社	十字花科植物から有効成分の分離方法
JPH02276574A (ja) *	1989-04-17	1990-11-13	Gakken Co Ltd	ワサビ培養細胞によるペルオキシダーゼ製造法及び培養物からのペルオキシダーゼ精製法
US5728550A (en) *	1990-01-15	1998-03-17	Phytera, Inc.	Peroxidase production
GB9119717D0 (en) *	1991-09-16	1991-10-30	Plant Science Ltd	Peroxidase producing plant cell cultures
US7390641B2 (en)	2004-05-25	2008-06-24	Council Of Scientific And Industrial Research	Production of peroxidase from plant cell and callus cultures

1982
- 1982-08-09 JP JP13869882A patent/JPS5928473A/ja active Granted

Also Published As

Publication number	Publication date
JPS5928473A (ja)	1984-02-15

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