JPH0143550B2 - - Google Patents

Info

Publication number: JPH0143550B2
Authority: JP; Japan
Prior art keywords: urokinase; perlite; human urine; urine; washed
Prior art date: 1981-06-26
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP9833881A

Other languages

English (en)

Japanese (ja)

Other versions

JPS58890A (ja

Inventor

Shigeji Yonezawa

Masato Nagata

Masao Takahashi

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Godo Shusei KK

Original Assignee

Godo Shusei KK

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1981-06-26

Filing date

1981-06-26

Publication date

1989-09-21

1981-06-26 Application filed by Godo Shusei KK filed Critical Godo Shusei KK

1981-06-26 Priority to JP9833881A priority Critical patent/JPS58890A/ja

1983-01-06 Publication of JPS58890A publication Critical patent/JPS58890A/ja

1989-09-21 Publication of JPH0143550B2 publication Critical patent/JPH0143550B2/ja

Status Granted legal-status Critical Current

Links

102000003990 Urokinase-type plasminogen activator Human genes 0.000 claims description 39
108090000435 Urokinase-type plasminogen activator Proteins 0.000 claims description 39
229960005356 urokinase Drugs 0.000 claims description 39
210000002700 urine Anatomy 0.000 claims description 22
238000000034 method Methods 0.000 claims description 19
239000010451 perlite Substances 0.000 claims description 15
235000019362 perlite Nutrition 0.000 claims description 15
238000001179 sorption measurement Methods 0.000 claims description 12
239000007788 liquid Substances 0.000 claims description 6
239000000243 solution Substances 0.000 claims description 6
229910001562 pearlite Inorganic materials 0.000 claims description 5
239000012266 salt solution Substances 0.000 claims description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 16
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 15
239000007864 aqueous solution Substances 0.000 description 11
102000004169 proteins and genes Human genes 0.000 description 11
108090000623 proteins and genes Proteins 0.000 description 11
239000011780 sodium chloride Substances 0.000 description 8
230000000694 effects Effects 0.000 description 7
238000010828 elution Methods 0.000 description 6
VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 5
239000003463 adsorbent Substances 0.000 description 5
239000012153 distilled water Substances 0.000 description 5
238000005187 foaming Methods 0.000 description 5
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
239000000126 substance Substances 0.000 description 4
239000000356 contaminant Substances 0.000 description 3
239000002244 precipitate Substances 0.000 description 3
102000009123 Fibrin Human genes 0.000 description 2
108010073385 Fibrin Proteins 0.000 description 2
BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
208000007536 Thrombosis Diseases 0.000 description 2
TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 description 2
229950003499 fibrin Drugs 0.000 description 2
150000003839 salts Chemical class 0.000 description 2
238000005406 washing Methods 0.000 description 2
206010014523 Embolism and thrombosis Diseases 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
-1 Hyflo Super Sale Chemical compound 0.000 description 1
BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical group [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
102000013566 Plasminogen Human genes 0.000 description 1
108010051456 Plasminogen Proteins 0.000 description 1
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
229910021536 Zeolite Inorganic materials 0.000 description 1
239000002246 antineoplastic agent Substances 0.000 description 1
229940041181 antineoplastic drug Drugs 0.000 description 1
239000000440 bentonite Substances 0.000 description 1
229910000278 bentonite Inorganic materials 0.000 description 1
SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
230000017531 blood circulation Effects 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
CGMRCMMOCQYHAD-UHFFFAOYSA-J dicalcium hydroxide phosphate Chemical compound [OH-].[Ca++].[Ca++].[O-]P([O-])([O-])=O CGMRCMMOCQYHAD-UHFFFAOYSA-J 0.000 description 1
HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
239000003814 drug Substances 0.000 description 1
229940088598 enzyme Drugs 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
239000012535 impurity Substances 0.000 description 1
229910052500 inorganic mineral Inorganic materials 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
239000011707 mineral Substances 0.000 description 1
208000010125 myocardial infarction Diseases 0.000 description 1
231100000957 no side effect Toxicity 0.000 description 1
230000002093 peripheral effect Effects 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
229940012957 plasmin Drugs 0.000 description 1
239000000843 powder Substances 0.000 description 1
239000011435 rock Substances 0.000 description 1
239000000741 silica gel Substances 0.000 description 1
229910002027 silica gel Inorganic materials 0.000 description 1
229940124597 therapeutic agent Drugs 0.000 description 1
239000010457 zeolite Substances 0.000 description 1

Landscapes

Enzymes And Modification Thereof (AREA)

JP9833881A 1981-06-26 1981-06-26 ウロキナ−ゼの回収法 Granted JPS58890A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP9833881A JPS58890A (ja)	1981-06-26	1981-06-26	ウロキナ−ゼの回収法

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP9833881A JPS58890A (ja)	1981-06-26	1981-06-26	ウロキナ−ゼの回収法

Publications (2)

Publication Number	Publication Date
JPS58890A JPS58890A (ja)	1983-01-06
JPH0143550B2 true JPH0143550B2 (de)	1989-09-21

Family

ID=14217111

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP9833881A Granted JPS58890A (ja)	1981-06-26	1981-06-26	ウロキナ−ゼの回収法

Country Status (1)

Country	Link
JP (1)	JPS58890A (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS61241171A (ja) *	1985-04-18	1986-10-27	Tokyo Electric Co Ltd	印字装置

1981
- 1981-06-26 JP JP9833881A patent/JPS58890A/ja active Granted

Also Published As

Publication number	Publication date
JPS58890A (ja)	1983-01-06

Publication	Publication Date	Title
CA2034826A1 (en)	1991-07-25	Process for the production of thrombin and high purity thrombin preparations thereby obtained
US3943245A (en)	1976-03-09	Purification of plasminogen
JP2521354B2 (ja)	1996-08-07	１ｙｓ―プラスミノ―ゲンの製造方法
US4066506A (en)	1978-01-03	Method of separating and purifying two active forms of urokinase using affinity chromatography
US4259447A (en)	1981-03-31	Process for the production of urokinase in pure condition
FI96211C (fi)	1996-05-27	Menetelmä yksisäikeisen kudosplasminogeeniaktivaattorin (tPA) ja kaksisäikeisen tPA:n puhdistamiseksi ja eristämiseksi
JPH0260312B2 (de)	1990-12-14
WO1987001389A1 (en)	1987-03-12	Methods for the recovery of tissue plasminogen activator
JPH0143550B2 (de)	1989-09-21
EP0040238B1 (de)	1984-07-11	Plasminogen-aktivatoren verwendbar als heil- und diagnosemittel und deren isolierung
US4189350A (en)	1980-02-19	Process for purifying urokinase
US4160697A (en)	1979-07-10	Method for purification of crude urokinase
USRE32271E (en)	1986-10-28	Isolation of plasminogen activators useful as therapeutic and diagnostic agents
US5015583A (en)	1991-05-14	Process for the purification of plasminogen activators (PA)
JPH0258913B2 (de)	1990-12-11
JPH0556951B2 (de)	1993-08-20
KR830001822B1 (ko)	1983-09-12	유로 키나제의 제조방법
JPS5816874B2 (ja)	1983-04-02	ウロキナ−ゼの精製法
JPS63317082A (ja)	1988-12-26	ｔＰＡの精製
JPS5932116B2 (ja)	1984-08-06	ウロキナ−ゼの精製法
US3884760A (en)	1975-05-20	Production of urokinase
KR790001376B1 (ko)	1979-09-29	우로키나제의 정제 및 회수법
JPH038196B2 (de)	1991-02-05
Banton et al.	1987	Fibrin Binding: An essential property of anisoylated plasminogen streptokinase activator complex
JPS5820268B2 (ja)	1983-04-22	ウロキナ−ゼ精製法