JPH02142481A - 新規組換えプラスミド、それを含む大腸菌、それを用いて得られるジヒドロ葉酸還元酵素及びその分離精製方法 - Google Patents

新規組換えプラスミド、それを含む大腸菌、それを用いて得られるジヒドロ葉酸還元酵素及びその分離精製方法

Info

Publication number
JPH02142481A
JPH02142481A JP29551988A JP29551988A JPH02142481A JP H02142481 A JPH02142481 A JP H02142481A JP 29551988 A JP29551988 A JP 29551988A JP 29551988 A JP29551988 A JP 29551988A JP H02142481 A JPH02142481 A JP H02142481A
Authority
JP
Japan
Prior art keywords
dhfr
dimer
enzyme
protein
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP29551988A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0364114B2 (de
Inventor
Masahiro Iwakura
正寛 巌倉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP29551988A priority Critical patent/JPH02142481A/ja
Publication of JPH02142481A publication Critical patent/JPH02142481A/ja
Publication of JPH0364114B2 publication Critical patent/JPH0364114B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP29551988A 1988-11-22 1988-11-22 新規組換えプラスミド、それを含む大腸菌、それを用いて得られるジヒドロ葉酸還元酵素及びその分離精製方法 Granted JPH02142481A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP29551988A JPH02142481A (ja) 1988-11-22 1988-11-22 新規組換えプラスミド、それを含む大腸菌、それを用いて得られるジヒドロ葉酸還元酵素及びその分離精製方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP29551988A JPH02142481A (ja) 1988-11-22 1988-11-22 新規組換えプラスミド、それを含む大腸菌、それを用いて得られるジヒドロ葉酸還元酵素及びその分離精製方法

Publications (2)

Publication Number Publication Date
JPH02142481A true JPH02142481A (ja) 1990-05-31
JPH0364114B2 JPH0364114B2 (de) 1991-10-03

Family

ID=17821672

Family Applications (1)

Application Number Title Priority Date Filing Date
JP29551988A Granted JPH02142481A (ja) 1988-11-22 1988-11-22 新規組換えプラスミド、それを含む大腸菌、それを用いて得られるジヒドロ葉酸還元酵素及びその分離精製方法

Country Status (1)

Country Link
JP (1) JPH02142481A (de)

Also Published As

Publication number Publication date
JPH0364114B2 (de) 1991-10-03

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Legal Events

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