JPH03280838A - Feed additive for ruminant - Google Patents

Abstract

PURPOSE:To obtain the title additive protecting a biologically active substance from gastric juice in rumen and efficiently absorbing said substance in digestive organs in and after abomasum by coating core granules containing a biologically active substance and a protective substance with a protecting coating film consisting of a specific salt of fatty acid and fats and oils, etc. CONSTITUTION:Core granules containing both a biologically active substance (preferably methionine, lysine hydrochloride or vitamins) and a protective substance (preferably the same kind of fatty acid salt as that of protecting coating film) are coated with a protecting coating film consisting of (A) a salt of fatty acid (preferably calcium salt of mixed fatty acid having 30-50 deg.C melting point) of fatty acid salt which is insoluble in a neutral range and shows decomposing properties in an acidic range and (B) fats and oils and/or wax to give the objective additive.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a feed additive for ruminants, and more particularly, it protects biologically active substances from the gastric juice of the rumen of ruminants. The present invention relates to a preparation in which a biologically active substance is coated with a protective coating of a fatty acid salt in order to be efficiently absorbed in the digestive organs starting from the four stomachs.

The feed additive for ruminants of the present invention can be added and mixed into concentrated feed and orally administered to ruminants such as cattle and sheep, and biologically active substances such as amino acids, proteins, and veterinary drugs can be efficiently absorbed. It is suitably used as a formulation for

[Conventional technology]

When biologically active substances such as amino acids, proteins, and veterinary drugs are orally administered to ruminants, they are not effectively absorbed because they are degraded by microorganisms present in the gastric juice of the rumen of the ruminants.

Therefore, in order to efficiently absorb these biologically active substances, the biologically active substances are coated and protected with a substance that is stable against the ruminal fluid, such as oil, and the digestive organs from the abomasum onward are protected. Various feed additives for ruminants that release biologically active substances have been proposed (Japanese Unexamined Patent Publication No. 56-15).
4956, Japanese Unexamined Patent Publication No. 151133/1983, etc.), the applicant of the present invention also added a biologically active substance to a protective substance such as hydrogenated oil, and determined the disintegrability of the protective substance in the digestive organs from the abomasum onwards. For the purpose of promoting this, a preparation protected by adding chitosan has been proposed and is marketed under the name Lafchit (see JP-A-58-175449, JP-A-59-198946, etc.).

On the other hand, as an energy source that bypasses the rumen of ruminants and is absorbed with high efficiency in the digestive organs after the abomasum, calcium of fatty acids having 14.16 and/or 18 carbon atoms,
Divalent metal salts such as magnesium have been proposed (USP 4,
826,694), and calcium salts of mixed fatty acids made from natural fats and oils (hereinafter referred to as "bypass fats and oils") are commercially available.

There is also known a method for producing feed additives for ruminants, in which these bypass fats and oils are melted, a biologically active substance is added thereto, and the mixture is then cooled and solidified (Japanese Patent Laid-Open No. 63-313).
(See Publication No. 546).

[Problem that the invention seeks to solve]

In feed additives for ruminants in which a biologically active substance is coated and protected with a protective substance such as the hydrogenated oil cited above, the ability of the biologically active substance to bypass the rumen and to release it in the digestive tract from the abomasum onwards is important. However, the storage stability, especially the thermal stability at temperatures above 40°C, is insufficient.

On the other hand, bypass oils and fats have a higher melting point and excellent thermal stability than hardened oils and the like. Therefore, preparations in which biologically active substances are coated and protected with bypass oils and fats can be expected to have excellent thermal stability, but formulations containing biologically active substances in high concentrations or those containing water-soluble biologically active substances can be expected to have excellent thermal stability. When a preparation containing a substance is produced by the above-mentioned method, various factors overlap and it is not possible to sufficiently impart ruminal bypass properties to the biologically active substance. The formulation is further coated with a protective coating of bypass oil, which allows the first biologically active substance to be
Gastric bypass properties can be improved, but in this case,
In order to form a dense protective film, it is necessary to dissolve the bypass oil in a solvent and coat it, which increases manufacturing costs. Furthermore, a protective film made only of bypass fats and oils needs to solve the problem of deterioration of the unsaturated fatty acids that constitute the bypass fats and oils.

An object of the present invention is to provide a feed additive for ruminants that has excellent rumen bypass properties for biologically active substances and improved stability.

[Means for solving problems]

As a result of intensive research to achieve the above object, the present inventors have discovered that the core granules containing biologically active substances are coated with a protective film consisting of fatty acid salts, oil, fat, wax, etc. using the method cited above. The present invention was completed based on the discovery that the ruminal bypass properties were significantly improved.

The present invention provides core granules containing biologically active substances and protective substances from the group consisting of (a) fatty acid salts that are insoluble in neutral ranges and degradable in acidic ranges, and (b) oils, fats, and waxes. A feed additive for ruminants characterized by being coated with a protective film comprising at least one selected species.

In the present invention, biologically active substances are substances that exhibit activities such as promoting fattening, preventing diseases, and treating diseases when given to animals. In particular, when orally administered to ruminants, biologically active substances are It is a substance that is decomposed by microorganisms present in

For example, amino acids: methionine, lysine, tryptophan, etc., N-acyl amino acids diN-stearoylmethionine, N-oleylmethionine.

Amino acid salts, such as the calcium salt of N-hydroxymethylmethionine: Hydroxy analogues of amino acids, such as lysine hydrochloride: 2-hydroxy-4-methylmercaptobutyric acid and its calcium salt, etc., Proteins: Fish powder, casein, potato Protein, soy protein, etc., vitamins: vitamin A, vitamin A acetate, vitamin A palmitate, vitamin D3. Vitamin E, nicotinic acid and nicotinamide, calcium pantothenate.

β-carotene, etc., enzymes: acid protease, etc., carbohydrates: glucose, etc., veterinary drugs: penicillin.

Examples include antibiotics such as tetracycline, anthelmintics such as Negfon, and one or more of these may be used.

The protective substance used as a binder during the production of nuclear granules is
It is stable in the rumen juice of ruminants, which has a neutral pH of 5 to 8, and is decomposed in the abomasal juice, which has a strongly acidic pH of 3 or less.
Any material that emulsifies with digestive juices from the stomach onwards is acceptable.

For example, linear or branched saturated or unsaturated monocarboxylic acids having 8 to 22 carbon atoms and their salts, hardened animal and vegetable oils, etc. may be mentioned, and one or more of these may be used. Preferably, divalent metal salts of linear or branched saturated or unsaturated fatty acids having 14 to 18 carbon atoms are used, and more preferably their calcium salts are used.

Nuclear granules are made by granulating one or more of the biologically active substances mentioned above and using a protective substance as a binder, and are usually prepared by uniformly mixing the biologically active substance powder and the protective substance, and then granulating them. It is manufactured by heating, softening, pelletizing, and cooling to solidify. As the granulation method, general granulation methods such as extrusion granulation method and stirring granulation method can be employed.

The fatty acid salt, which is a component of the protective film, is insoluble in the neutral range and decomposable in the acidic range, and like the above-mentioned protective substances, it is a fatty acid salt that is stable in the rumen juice of ruminants and decomposed in the abomasal juice. , for example, the carbon number 14.16 cited above and /
Alternatively, calcium salts or magnesium salts of 18 fatty acids, calcium salts of mixed fatty acids produced from natural oils and fats, etc. are used.

Preferably the melting point is 30 to 50°C, more preferably 35°C.
Use calcium salts of mixed fatty acids at ~45°C.

Also, as one component of the protective film, oils and fats, ie, triglycerides of the aforementioned fatty acids, waxes such as bran wax, and the like are used. In particular, triglycerides having a melting point in the range of 55 to 80°C are preferably used.

The protective film is easily formed by coating the core granules using a coating machine using a slurry in which the fatty acid salt powder is dispersed in the molten triglyceride or the like as a coating liquid.

[For production]

As described above, the present invention covers core granules containing biologically active substances with a protective film consisting of a fatty acid salt, oil, fat, wax, etc., which is insoluble in a neutral range and degradable in an acidic range. This is a feed additive for ruminants characterized by the following.

In the present invention, since the protective film is water-insoluble in the neutral range, it is extremely stable in the ruminal fluid of ruminants in the pH range of 5 to 8, and its component fatty acid salt is in the acidic range. Since it is degradable, it is easily broken down in the abomasum of ruminants at pH 3 or lower.

In addition, by using oils, waxes, etc. as components of the protective film, the film quality becomes denser than that of a film formed only with fatty acid salts, and the elution rate of biologically active substances in the rumen decreases, resulting in a reduction in the rate of elution of biologically active substances in the rumen. rate is improved. As a result, the biologically active substances contained in the nuclear granules are eluted in the abomasum of the ruminant and are efficiently absorbed in the subsequent digestive organs. The coating material itself is also absorbed in the digestive organs starting from the abomasum and is used as an energy source for ruminants.

In addition, the temperature at which a protective film is formed on the core granules is lower, at 55-80°C, which is the melting point temperature of oils, fats, waxes, etc., compared to 120-130°C when forming a protective film of only fatty acid salts. , not only the thermal decomposition of biologically active substances is prevented, but also the workability is improved.

Furthermore, by using a protective substance that is itself insoluble in a neutral range and decomposable in an acidic range as a binder during the production of core granules, the rumen bypass properties of biologically active substances are improved.

Furthermore, the bypass oil used as a binder for the core granules and a component of the protective film has strong water repellency and heat resistance, which prevents deterioration of the formulation itself even when the formulation is stored at high temperatures of 40 to 50°C. and the rumen bypass properties of biologically active substances are also maintained.

〔Example〕

The present invention will be explained in more detail by Examples and Comparative Examples.

However, the scope of the present invention is not limited in any way by the following examples.

In addition, in the following steel, "parts" and "%" are based on weight unless otherwise specified.

(1) Preparation of core granules Calcium salt of beef tallow fatty acid with a melting point of 43°C
Heat to 40°C to soften, add methionine powder or risine hydrochloride powder, knead for about 10 minutes, cool to room temperature, crush and sieve to obtain core granules with a particle size of 0.5 to 3 mm. was prepared.

The composition and particle size of the obtained core granules are shown in Table 1.

(2) Preparation of feed additive for ruminants 1 kg of the core of the core granules prepared in the above item (1) was placed in a coating device (Henschel mixer) and heated to 65 to 70° C. while stirring. Adding the same fatty acid calcium powder and hydrogenated beef tallow oil with a melting point of 60°C to this as used in item (1) above, stirring was continued to form a protective film on the surface of the core granules to coat them. , prepared a feed additive for ruminants.

In addition, for the core granules prepared in the above item (1), the heating temperature was set to 120 to 130°C, and when the surface of the core granules had started to melt slightly, the same as that used in the above item (1) was heated. Stirring was continued while adding fatty acid calcium powder to form a protective film consisting only of fatty acid calcium on the surface of the core granules, thereby preparing a comparative formulation.

The coating amount and biologically active substance content of the obtained feed additive for ruminants are shown in Table 1.

(3) Dissolution test of biologically active substances 2 g of each of the ruminant feed additive prepared in the above item (2) and the nuclear granules prepared in the above item (1) for comparison were administered to cattle. It was immersed in 200 cc of Tris buffer corresponding to the rumen juice of a cow and kept shaking for 24 hours at a temperature of 37°C. ! ・dm-3
) Immersed in 200cc of hydrochloric acid and heated at 37℃ for 4 more hours.
Shake for an hour. Then, the preparation taken out from 0.05 M hydrochloric acid was immersed in 200 cc of a solution corresponding to bovine small intestine, and further shaken at a temperature of 37° C. for 4 hours.

Tris buffer Tris [tris(hydroxymethyl)aminomethane]
6.06g, 292mi! A solution of pH 8.0 was dissolved in 0.1 M hydrochloric acid and diluted to 1000 ml with water, and then the methionine and lysine eluted in Tris buffer, 0.05 M hydrochloric acid, and the corresponding small intestine solution were measured by iodo titration method or ninhydrin color method. It was quantified by

The test results, Shown in Table 1.

As shown in Table 1, in the feed additive for ruminants coated with the fatty acid calcium film of the present invention, the dissolution rate of biologically active substances in the rumen-compatible fluid is extremely low, and Most of the remainder is eluted in the corresponding solution and the small intestine solution.

On the other hand, in preparations coated only with fatty acid calcium and in preparations without coating (see comparative example), the dissolution rate of biologically active substances in the ruminal fluid was high, and in particular in preparations without coating, biologically active substances 1 of the chemically active substances
Only about 0-15% bypass the rumen.

〔Effect of the invention〕

As shown in the examples above, the feed additive for ruminants of the present invention has extremely excellent rumen bypass properties for the biologically active substances contained therein when orally administered to ruminants.

Furthermore, the fatty acid salt used to impart bypass properties is also decomposed and absorbed in the digestive organs starting from the abomasum, and is effectively used as an energy source for ruminants.

The present invention is directed to a ruminant animal suitable for bypassing the rumen and absorbing biologically active substances that are easily degraded in the rumen of the ruminant when administered orally and with high efficiency in the digestive organs from the abomasum onwards. It provides feed additives for livestock, and its significance in industry, especially in the livestock field, is extremely large.

Claims (4)

[Claims] (1) Core granules containing biologically active substances and protective substances are selected from the group consisting of (a) fatty acid salts that are insoluble in neutral ranges and degradable in acidic ranges, and (b) oils, fats, and waxes. A feed additive for ruminants characterized by being coated with a protective film consisting of at least one type of (2) A feed additive for ruminants according to claim (1), wherein the biologically active substance is at least one selected from the group consisting of methionine, lysine hydrochloride, and vitamins. (3) The feed additive for ruminants according to claim (1), wherein the protective substance is a fatty acid salt of the same type as the fatty acid salt that is a component of the protective film. (4) The feed additive for ruminants according to claim (1), wherein the fatty acid salt that is a component of the protective film is a calcium salt of mixed fatty acids having a melting point in the range of 30 to 50°C.