JPH03280841A - Feed additive for ruminant - Google Patents

Abstract

PURPOSE:To obtain the title additive protecting a biologically active substance from gastric juice in rumen and efficiently absorbing said substance in digestive organs in and after abomasum by coating core granules molded from a biologically active substance and a protective substance with a specific protecting coating film and further with a titanium oxide-containing coating film. CONSTITUTION:Core granules containing both a biologically active substance (preferably methionine, lysine hydrochloride or vitamins) and a protective substance (preferably bivalent metallic salt of mixed fatty acid having 30-50 deg.C melting point and water-soluble higher fatty acid, etc.) are coated with a protecting coating film consisting of a salt of fatty acid (preferably bivalent metallic salt of a mixed fatty acids having 30-50 deg.C melting point) insoluble in a neutral range and shows decomposing properties in an acidic range and a water- insoluble substance (preferably higher fatty acid, higher alcohol or glycerin mono-fatty acid ester) having compatibility with the salt of fatty acid and further with a titanium oxide-containing coating film to give the objective additive.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a feed additive for ruminants, and more particularly, it protects biologically active substances from the gastric juice of the rumen of ruminants. In order to efficiently absorb the biologically active substance in the digestive organs starting from the stomach, the core granules containing the biologically active substance are coated with a protective film consisting of a fatty acid salt and a water-insoluble substance that is compatible with the fatty acid salt, and further coated with a titanium oxide-containing film. Concerning coated formulations.

The feed additive for ruminants of the present invention is added to feed and mixed,
It can be orally administered to ruminants such as cattle and sheep, and is suitably used as a preparation for efficiently absorbing biologically active substances such as amino acids, proteins, and veterinary drugs.

[Conventional technology]

When biologically active substances such as amino acids, proteins, and veterinary drugs are orally administered to ruminant animals, they are decomposed by microorganisms present in the gastric juice of the rumen of the rumen, and are not absorbed as is.

Therefore, in order to efficiently absorb these biologically active substances, the biologically active substances are coated and protected with a substance that is stable against the ruminal fluid, such as oil, and the digestive organs from the abomasum onward are protected. Various feed additives for ruminants that release biologically active substances have been proposed (Japanese Unexamined Patent Publication No. 56-15).
4956, Japanese Unexamined Patent Publication No. 151133/1983, etc.), the applicant of the present invention also added a biologically active substance to a protective substance such as hydrogenated oil, and determined the disintegrability of the protective substance in the digestive organs from the abomasum onwards. For the purpose of promoting this, a preparation protected by adding chitosan has been proposed and is marketed under the name Lafchit (see JP-A-58-175449, JP-A-59-198946, etc.).

On the other hand, as an energy source that bypasses the rumen of ruminants and is absorbed with high efficiency in the digestive organs from the abomasum onwards, divalent fatty acids such as calcium and magnesium containing 14 carbon atoms, 6 and/or 18 carbon atoms, and Metal salts were proposed (U.S.
P 4.826.694), calcium salts of mixed fatty acids made from natural fats and oils (hereinafter referred to as "bypass fats") are commercially available.

In addition, there is a known method for producing feed additives for ruminants, in which these bypass fats and oils are heated to a temperature above their melting point to soften them, a biologically active substance is added and mixed therein, and then the mixture is cooled, solidified, and crushed. (Refer to Japanese Patent Laid-Open No. 63-313546).

[Problem that the invention seeks to solve]

In feed additives for ruminants in which biologically active substances are coated and protected with the above-mentioned protective substances such as hydrogenated oil, the biologically active substances have ruminal bypass properties and are released in the digestive tract from the abomasum onward. However, there is a need to improve storage stability, especially thermal stability when stored at 40°C or higher.

On the other hand, bypass oils and fats have a higher melting point and excellent thermal stability than hardened oils and the like. Therefore, a preparation in which a biologically active substance is coated and protected with a bypass oil can be expected to have excellent thermal stability, but the method described in the cited document in which a biologically active substance is simply mixed with a heat-softened bypass oil Due to a combination of various factors, the ruminal bypass properties of biologically active substances are poor in preparations manufactured with 2-50% fluoride, especially in preparations containing high amounts of biologically active substances or those containing highly water-soluble biologically active substances. In preparations containing biologically active substances, it is difficult to sufficiently cover biologically active substances with a protective film of bypass oil. f: f<l+)-su,)'a
m++f # The unsaturated fatty acids that make up bypass fats and oils are unstable to oxygen and light in the air, so they lack storage stability.

An object of the present invention is to provide a feed additive for ruminants that uses as a protective substance a bypass fat and oil that exhibits excellent ruminal binostatic properties for biologically active substances.

[Means for solving problems]

As a result of intensive research to achieve the above object, the present inventors have discovered that core granules prepared by using biologically active substances as bypass oils and fats and water-insoluble fatty acids, fats, oils, wax, etc. and coating with a protective substance consisting of a water-insoluble substance that is compatible with the bypass oil and fat, the ruminal bypass property of the biologically active substance is significantly improved, and further, the coated preparation is coated with a titanium oxide-containing film. This significantly improves storage stability.1. - Constructed a big invention.

The present invention provides a repellent consisting of a fatty acid salt that is insoluble in a neutral range and decomposable in an acidic range, and a water-insoluble substance that is compatible with this fatty acid salt, in core granules formed from a biologically active substance and a protective substance. Covered with a dense protective film that is highly water-based,
The feed additive for ruminants is further characterized in that it is coated with a film containing titanium oxide.

In the present invention, biologically active substances are substances that exhibit activities such as promoting fattening, preventing diseases, and treating diseases when given to animals. In particular, when orally administered to ruminants, biologically active substances are It is a substance that is decomposed by microorganisms present in the body, and is difficult to exert its effects as it is.

For example, amino acids: methionine, lysine, tryptophan, etc., N-acyl amino acids diN-stearoylmethionine, N-oleylmethionine.

Salts of amino acids, such as calcium salts of N-hydroxymethylmethionine: Hydroxy analogues of amino acids, such as lysine hydrochloride: 2-hydroxy-4-methylmercaptobutyric acid and its calcium salts, etc., Proteins: No powder, casein, potato Protein, soy protein, etc., vitamins: vitamin A, vitamin A acetate, vitamin A palmitate, vitamin D3+, vitamin E, nicotinic acid and nicotinamide, calcium pantothenate, β-carotene, etc., enzymes: acid protease etc,
Carbohydrates: glucose, etc. Veterinary medicines: antibiotics such as penicillin and tetracycline, anthelmintics such as Negfon, etc., and one or more of these are used.

The protective substance used as a binder during the production of nuclear granules is
If it is stable in the rumen juice of ruminants in the neutral pH range of 5 to 8, and is decomposed or emulsified in the abomasal gastric juice in the acidic range of pH 3 or lower or in the digestive juices of the digestive organs after the abomasum. good.

For example, the metal salt of a straight chain or branched saturated or unsaturated fatty acid having 8 to 22 carbon atoms is preferably a divalent metal salt, and more preferably a calcium salt. These can be used alone, but include straight chain or branched saturated or unsaturated monocarboxylic acids with 14 to 22 carbon atoms, saturated or unsaturated monocarboxylic acids with straight chain or branching of 14 to 22 carbon atoms, It is also possible to use it in combination with one or more of alcohols, fatty acid esters of glycerin, hardened animal and vegetable oils, etc.

The mixing ratio is not particularly specified, but the carbon number is 8 to 22.
It is necessary to use the metal salt of a saturated or unsaturated fatty acid having a linear or branched chain in an amount of at least 30% by weight, and the melting point of the protective substance is preferably at least 80°C or higher. Furthermore, inorganic fillers such as calcium carbonate may be added to adjust the specific gravity of the preparation.

Nuclear granules can be produced by mixing one or more of the biologically active substances described above with a protective substance and using a conventional granulation method. As a manufacturing method, a spray granulation method, an extrusion granulation method, an agitation granulation method, etc. can be used.

The fatty acid salt used for the protective film is insoluble in a neutral range and degradable in an acidic range, and is a salt of a fatty acid that is stable in the ruminal juice of a ruminant and decomposed in the abomasal juice, similar to the above-mentioned protective substance. , for example, the carbon number 14.16 cited above and /
Alternatively, calcium salts of 18 fatty acids, calcium salts of mixed fatty acids produced from natural oils and fats, etc. are used.

Preferably the melting point is 30 to 50°C, more preferably 35°C.
Calcium salts of mixed fatty acids at ~45°C are used.

Water-insoluble substances that are compatible with the fatty acid salt, which is one of the components of the protective film, include, for example, saturated or unsaturated linear or branched fatty acids and alcohols having 8 to 22 carbon atoms, glycerin monofatty acid esters, etc. etc. are used. In particular, the aforementioned fatty acids, alcohol glycerol monofatty acid esters and mixtures thereof having melting points in the range from 55 to 80° C. are used. Further, in order to further improve the rumen bypass property, hardened animal and vegetable oils, wax, etc. can be added. There is no particular restriction on the proportion of these additions, but when the melting point of the protective substance is measured, it is preferably 80°C or higher.

The protective coating and the titanium oxide-containing coating are formed using conventional coating equipment, such as a fluidized bed type or transfer type equipment. The core granules used at this time are preferably rounded from the viewpoint of coating efficiency.

A titanium oxide-containing film is a film in which titanium oxide powder is mixed and contained in a film-forming substance.

As the film-forming substance, for example, water-soluble cellulose derivatives such as hydroxypropyl cellulose (hereinafter referred to as rHPCJ) and hydroxypropyl methyl cellulose are preferably used, and are expected to provide flexibility and adhesion to the first protective film. For this purpose, polyethylene glycol (hereinafter referred to as rPEG), glycerin, etc. are added.

The titanium oxide-containing film is formed using a coating device,
It is formed by using a slurry in which a film-forming substance, powder of titanium oxide, and additives such as PEG are dissolved and dispersed in water as a coating liquid, and coating the preparation coated with the protective film of the fatty acid salt.

[For production]

In the present invention, during the production of core granules, fatty acid salts, higher fatty acids, higher alcohols, which are insoluble in a neutral region and decomposable in an acidic region,
By using fats and oils in combination, denser core granules can be obtained than by molding with fatty acid salt alone, and the rumen bypass properties of ruminants are also improved.

In addition, by using fatty acid salts that are insoluble in neutral ranges and decomposable in acidic ranges as protective substances, fatty acids, higher alcohols, glycerin monofatty acid esters, etc. that are compatible with these salts, the film is compared to films formed only with fatty acid salts. , the membrane quality becomes dense, the ability of biologically active substances to bypass the rumen is improved, and the release ability of the abomasum is also improved. Furthermore, the melting point is 80℃
Because of the above, storage stability is also improved.

In the present invention, since the fatty acid salt that is a component of the protective film is insoluble in a neutral range and degradable in an acidic range,
It is extremely stable in the rumen gastric fluid of ruminants with a pH in the range of 5 to 8, and is easily decomposed in the abomasum of ruminants with a pH of 3 or less. As a result, the biologically active substances contained in the nuclear granules are eluted in the abomasum of the ruminant and are efficiently absorbed in the subsequent digestive organs.

In addition, by using water-insoluble substances that are compatible with fatty acid salts such as fatty acids, alcohols, and glycerin monofatty acid esters as components of the protective film, the film quality becomes denser than that of a film formed only with fatty acid salts, and biological The dissolution rate of the active substance in the rumen is reduced and the bypass rate is improved.

As a result, the biologically active substances contained in the nuclear granules are eluted in the abomasum of the ruminant and are efficiently absorbed in the subsequent digestive organs.

Furthermore, the titanium oxide-containing film of the outer layer not only blocks oxygen and prevents oxidation of the protective film, but also prevents deterioration due to light because the titanium oxide it contains blocks light. As a result, storage stability is improved.

〔Example〕

The present invention will be explained in more detail by Examples and Comparative Examples.

However, the scope of the present invention is not limited in any way by the following examples.

In addition, in the following steel, "parts" and "%" are based on weight unless otherwise specified.

(1) Preparation of core granules Calcium salt of beef tallow fatty acid with a melting point of 43°C
After heating to 0°C to melt and soften, add castor hydrogenated fatty acid and methionine powder and knead for about 10 minutes.
Extruded and cut at a temperature of 90-110℃, particle size 1.0
−3.4 mn+ nuclear granules Al and A-2 were prepared.

Similarly, after mixing calcium salt of tallow fatty acid with a melting point of 43°C, stearic acid, calcium carbonate, and lysine hydrochloride powder or vitamin E, the mixture is kneaded, extruded, and cut to form core granules with a particle size of 1.0 to 1.2 mm. A-3 and A-4 were prepared.

In addition, a calcium salt of beef tallow fatty acid with a melting point of 43 degrees Celsius was dissolved in a mixture of stearic acid and beef tallow hydrogenated oil heated and melted at 120 degrees Celsius, and a slurry prepared by adding and mixing lysine hydrochloride powder and calcium carbonate was sprayed to solidify the particle size. A nuclear granule A-5 of 0.6-0.8 strength was prepared.

The composition, melting point and particle size of the obtained core granules are shown in Table 1.

(2) Preparation of coated preparation with protective film Core granules prepared in the above item (1): A-1 to A-5-1
kg into a Henschel mixer, and while stirring, add a 1200C melt of castor hydrogenated fatty acid or castor hydrogenated fatty acid and stearic acid in which the same fatty acid calcium salt as used in item (1) above was dissolved, or the same A protective film was formed on the surface of the core granules by spraying a 120° C. melt containing stearic acid in which a fatty acid calcium salt was dissolved and hydrogenated rapeseed oil.

The composition, coating amount, softening point/melting point, and biologically active substance content of the coating layer of the obtained preparation are shown in Table 2.

(3) Coating machine with titanium oxide-containing film (Hikoter Freund Sangyo Co., Ltd.)
1k each of the coated preparation prepared in section (2) above and the core granules: A-2 (for comparison) prepared in section (1)
A titanium oxide-containing film was formed using a coating solution having the following composition on G. g to prepare a feed additive for ruminants.

[Coating liquid composition]

titanium oxide powder hydroxypropyl cellulose (HPC: 6C HPC-8L) polyethylene glycol (PEG: Macrogol 6000) water Weight part 4.8 16,0 ■.

Table 2 shows the composition of the titanium oxide-containing film of the ruminant feed additive obtained.

(3) Dissolution test of biologically active substances 2 g each of the ruminant feed additive prepared in the above item (3) and the core granules prepared in the above item (1) for comparison
and Tris buffer 200c corresponding to bovine ruminal fluid.
After shaking and holding at a temperature of 37°C for 24 hours, it was taken out from the Tris buffer and 0.05M (=m) corresponding to bovine abomasal fluid.

1-dm-'') in 200 cc of hydrochloric acid and shaken at a temperature of 37°C for an additional 4 hours.Then, the preparation taken out from the 0.05 M hydrochloric acid was immersed in 200 cc of a fluid corresponding to the small intestine of a cow, and then shaken at a temperature of 37°C. Shake for an additional 4 hours.

Tris buffer Tris [) Lis(hydroxymethyl)aminomethane (36.06 g) was dissolved in 292 ml of 0.1 M hydrochloric acid and diluted to 1000 ml with water to a pH of 8.0, followed by Tris buffer and 0.05 M hydrochloric acid. And methionine and lysine eluted into the small intestine fluid were quantified by iodine titration or ninhydrin color method. Quantification of vitamin E was performed by high performance liquid chromatography.

The test results are shown in Table 2.

(4) Storage stability test 2 g each of the ruminant feed additive prepared in the above item (3) and the core granules prepared in the above item (1) for comparison.
was left in direct sunlight for 80 days.

The POV and biologically active substance decomposition rate were measured for each sample after storage.

The measurement results are shown in Table 2 along with changes in appearance.

As shown in Table 2, the ruminant feed additive of the present invention shows no deterioration even when stored in a normal workplace, and has the same effect on ruminal fluid as when stored in a cool dark place. The elution rate of the biologically active substance is extremely low, and most of the remainder is eluted in the fluid corresponding to the abomasum and the small intestine.

On the other hand, in formulations coated only with a titanium oxide-containing film and formulations without coating (see Comparative Examples), the dissolution rate of biologically active substances in the ruminal fluid is high.

〔Effect of the invention〕

As shown in the examples above, the feed additive for ruminants of the present invention not only has extremely excellent ruminal bypass properties for the biologically active substances contained therein when orally administered to ruminants. It also has excellent storage stability.

The present invention provides biologically active substances that are easily degraded in the rumen of ruminants when administered orally, and are suitable for bypassing the rumen and absorbing them with high efficiency in the digestive organs from the abomasum onwards.
The present invention provides a feed additive for ruminants with excellent storage stability, and has extremely great significance in industry, particularly in the livestock field.

Claims (5)

[Claims] (1) A protective coating consisting of a fatty acid salt that is insoluble in a neutral range and decomposable in an acidic range and a water-insoluble substance that is compatible with this fatty acid salt is formed on the core granule formed from a biologically active substance and a protective substance. A feed additive for ruminants characterized by being coated with a film containing titanium oxide and further coated with a film containing titanium oxide. (2) A feed additive for ruminants according to claim (1), wherein the biologically active substance is at least one selected from the group consisting of methionine, lysine hydrochloride, and vitamins. (3) The feed additive for ruminants according to claim (1), wherein the fatty acid salt forming the protective film is a divalent metal salt of a mixed fatty acid having a melting point in the range of 30 to 50°C. (4) In claim (1), the protective substance has a melting point of 3
A product for ruminants comprising a divalent metal salt of a mixed fatty acid in the range of 0 to 50°C and at least one member selected from the group consisting of water-insoluble higher fatty acids, higher alcohols, fatty acid esters of glycerin, fats and oils, and waxes. feed additives (5) A feed additive for ruminants according to claim (1), wherein the water-insoluble substance compatible with the fatty acid salt is at least one selected from the group consisting of higher fatty acids, higher alcohols, and glycerin monofatty acid esters. agent