JPH03280839A - Feed additive for ruminant - Google Patents

Abstract

PURPOSE:To obtain the title additive protecting a biologically active substance from gastric juice in rumen and efficiently absorbing said substance in digestive organs in and after abomasum by doubly coating core granules containing a biologically active substance and a protective substance with a specific protect ing coating film and a titanium oxide containing coating film. CONSTITUTION:Core granules containing both a biologically active substance (preferably methionine, lysine hydrochloride or vitamins) and a protective sub stance (preferably the same kind of fatty acid salt as component of protecting coating film) are doubly coated with a protecting coating film consisting of (A) a salt of fatty acid (preferably calcium salt of a mixed fatty acids having 40-50 deg.C melting point) which is insoluble in a neutral range and shows decompos ing properties in an acidic range and (H) fats and oils and/or wax and a tita nium oxide-containing coating film (preferably coating film obtained by dispers ing powder, flakes, etc., of titanium oxide into water-soluble cellulose derivative) to give the objective additive.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a feed additive for ruminants, and more particularly, it protects biologically active substances from the gastric juice of the rumen of ruminants. The present invention relates to a preparation in which a biologically active substance is coated with a protective coating of a fatty acid salt in order to be efficiently absorbed in the digestive organs starting from the four stomachs.

The feed additive for ruminants of the present invention is added to feed and mixed,
It can be orally administered to ruminants such as cattle and sheep, and is suitably used as a preparation for efficiently absorbing biologically active substances such as amino acids, proteins, and veterinary drugs.

[Conventional technology]

When biologically active substances such as amino acids, proteins, and veterinary drugs are orally administered to ruminants, they are decomposed by microorganisms present in the gastric fluid of the rumen of the ruminant, and are not absorbed as is.

Therefore, in order to efficiently absorb these biologically active substances, the biologically active substances are coated and protected with a substance that is stable against the ruminal fluid, such as oil, and the digestive organs from the abomasum onward are protected. Various feed additives for ruminants that release biologically active substances have been proposed (Japanese Unexamined Patent Publication No. 56-15).
4956, Japanese Unexamined Patent Publication No. 151133/1983, etc.), the applicant of the present invention also added a biologically active substance to a protective substance such as hydrogenated oil, and determined the disintegrability of the protective substance in the digestive organs from the abomasum onwards. We propose a formulation protected by adding chitosan for the purpose of promoting
] It is published under the name.

On the other hand, divalent metals such as calcium and magnesium of fatty acids with 14.1G and/or 18 carbon atoms are used as an energy source that bypasses the rumen of ruminants and is absorbed with high efficiency in the digestive organs after the abomasum. Salt was proposed (US
P 4,826,694), calcium salts of mixed fatty acids made from natural fats and oils (hereinafter referred to as "bypass fats") are commercially available.

In addition, there is a known method for producing feed additives for ruminants, in which these bypass fats and oils are heated to a temperature above their melting point to soften them, a biologically active substance is added and mixed therein, and then the mixture is cooled, solidified, and crushed. (Refer to Japanese Patent Laid-Open No. 63-313546).

[Problem that the invention seeks to solve]

In feed additives for ruminants in which biologically active substances are coated and protected with the above-mentioned protective substances such as hydrogenated oil, the biologically active substances have ruminal bypass properties and are released in the digestive tract from the abomasum onwards. Excellent properties and storage stability, especially 40%
There is a need for improved thermal stability when stored at temperatures above °C.

On the other hand, bypass oils and fats have a higher melting point and excellent thermal stability than hardened oils and the like. Therefore, it is expected that a preparation in which a biologically active substance is coated and protected with a bypass oil or fat has excellent thermal stability. Due to a combination of various factors, the ruminal bypass properties of biologically active substances are poor in preparations manufactured with 2-50% fluoride, especially in preparations containing high amounts of biologically active substances or those containing highly water-soluble biologically active substances. In preparations containing the bypass oil, it is difficult to sufficiently cover the biologically active substance with the protective film of the bypass oil, so the biologically active substance hardly exhibits ruminal bypass properties, and Because the unsaturated fatty acids that make up these ingredients are unstable to oxygen and light in the air, they lack storage stability.

An object of the present invention is to provide a feed additive for ruminants that uses as a protective substance a bypass oil and fat that has excellent ruminal bypass properties for biologically active substances.

[Means for solving problems]

As a result of intensive research to achieve the above object, the present inventors have discovered that biologically active substances can be activated by coating core granules containing biologically active substances with a protective film made of fatty acid salts, oils, fats, waxes, etc. The inventors have completed the present invention by discovering that the rumen bypass properties of the substance are significantly improved, and that the storage stability is significantly improved by coating the coated preparation with a titanium oxide-containing film.

The present invention provides core granules containing a biologically active substance and a protective substance, comprising (a) a fatty acid salt that is insoluble in a neutral range and decomposable in an acidic range, and (b) a group consisting of oils, fats, and waxes. This feed additive for ruminants is characterized by being coated with two layers: a protective film consisting of one type selected from the following, and a titanium oxide-containing film.

In the present invention, the biologically active substance is a substance that is given to animals and exhibits activities such as promoting fattening, preventing diseases, and treating diseases. It is a substance that is decomposed by the microorganisms present in the body, and is difficult to exert its effects as it is.

For example, amino acids: methionine, lysine, tryptophan, etc., N-acyl amino acids diN-stearoylmethionine, N-oleylmethionine.

Salts of amino acids, such as calcium salts of N-hydroxymethylmethionine: Hydroxy homologues of amino acids, such as lysine hydrochloride = 2-hydroxy-4-methylmercaptobutyric acid and its calcium salts, etc., Proteins: Powder-free, casein, potato Protein, soy protein, etc., vitamins: vitamin A, vitamin A acetate, vitamin A palmitate, vitamin D31, vitamin E, nicotinic acid and nicotinamide, calcium pantothenate, β-carotene, etc., enzymes: acidic protease etc,
Carbohydrates: glucose, etc. Veterinary medicines: antibiotics such as penicillin and tetracycline, anthelmintics such as Negfon, etc., and one or more of these are used.

The protective substance, which is a component of the nuclear granule, is stable in the ruminal fluid of ruminants, which has a neutral pH of 5 to 8, and is stable in the ruminal fluid of ruminants, which has a neutral pH of 5 to 8, and is stable in the ruminal fluid of ruminants, which has a neutral pH of 5 to 8. Any material that can be broken down by digestive juices is fine.

For example, linear or branched saturated or unsaturated monocarboxylic acids having 8 to 22 carbon atoms and their salts, hardened animal and vegetable oils, etc. may be mentioned, and one or more of these may be used. Preferably, divalent metal salts of linear or branched saturated or unsaturated fatty acids having 14 to 18 carbon atoms are used, and more preferably their calcium salts are used.

As a component of the core granule, in addition to the biologically active substance and protective substance, inorganic powders such as calcium carbonate, magnesium carbonate, calcium phosphate, etc. can be added as a specific gravity regulator, if desired.

Core granules are made by granulating one or more of the above-mentioned biologically active substances and a specific gravity regulator added if desired, using a protective substance as a binder, and are usually prepared after uniformly mixing each component. It is manufactured by heating, softening, pelletizing, cooling and solidifying.

As the granulation method, a conventional granulation method such as an extrusion granulation method or an agitation granulation method can be employed.

Preferably, the softened mixture is extruded into strings by extrusion granulation, and then hot-cut to granulate. The shape of the granules is preferably closer to a true sphere than spherical, elliptical, etc.

The fatty acid salt used for the protective film is insoluble in a neutral range and degradable in an acidic range, and is a salt of a fatty acid that is stable in the ruminal juice of a ruminant and decomposed in the abomasal juice, similar to the above-mentioned protective substance. , for example, the carbon number 14.16 cited above and /
Alternatively, calcium salts of 18 fatty acids, calcium salts of mixed fatty acids produced from natural oils and fats, etc. are used.

Preferably the melting point is 30 to 50°C, more preferably 35°C.
Calcium salts of mixed fatty acids at ~45°C are used.

Also, as one component of the protective film, oils and fats, ie, triglycerides of the aforementioned fatty acids, waxes such as bran wax, and the like are used. In particular, triglycerides having a melting point in the range of 55 to 80°C are preferably used.

The protective film is easily formed by coating the core granules using a coating machine using a slurry in which the fatty acid salt powder is dispersed in the molten triglyceride or the like as a coating liquid.

A titanium oxide-containing film is a film in which titanium oxide powder is mixed and contained in a film-forming substance.

As the film-forming substance, water-soluble cellulose derivatives such as hydroxypropyl cellulose (hereinafter referred to as rHPc) and hydroxypropyl methylcellulose are preferably used, and further impart flexibility and adhesion to the protective film of the second layer. For this purpose, polyethylene glycol (hereinafter referred to as rPEGj), glycerin, etc. are added.

The titanium oxide-containing film is formed using a coating device,
It is formed by using a slurry in which a film-forming substance, powder of titanium oxide, and additives such as PEG are dissolved and dispersed in water as a coating liquid, and coating the preparation coated with the protective film of the fatty acid salt.

The protective coating and the titanium oxide-containing coating are formed using conventional coating equipment, such as a fluidized bed type or transfer type equipment. The core granules used at this time are preferably rounded from the viewpoint of coating efficiency.

[For production]

As described above, the present invention covers core granules containing biologically active substances with a protective film consisting of a fatty acid salt, oil, fat, wax, etc., which is insoluble in a neutral range and degradable in an acidic range. This feed additive for ruminants is further coated with a titanium oxide-containing film.

In the present invention, since the fatty acid salt that is a component of the protective film is insoluble in a neutral range and degradable in an acidic range,
It is extremely stable in the gastric fluid of the rumen of ruminants with a pH in the range of 5 to 8, and is easily degraded in the abomasum of ruminants with a pH of 3 or less. As a result, the biologically active substances contained in the nuclear granules are eluted in the abomasum of the ruminant and are efficiently absorbed in the subsequent digestive organs.

Furthermore, by using a protective substance that is itself insoluble in a neutral range and decomposable in an acidic range as a binder during the production of core granules, the rumen bypass properties of biologically active substances are improved. In particular, by using the same type of fatty acid calcium as the coating material, the binding force between the core granules and the coating is strengthened, and as a result, the destruction of the preparation due to rumination is reduced, further increasing the ruminal bypass rate of biologically active substances. improves.

In addition, by using oils, waxes, etc. as components of the protective film, the film quality becomes denser than that of a film formed only with fatty acid salts, which reduces the elution rate of biologically active substances in the rumen and improves the bypass rate. do. As a result, the biologically active substances contained in the nuclear granules are eluted in the abomasum of the ruminant and are efficiently absorbed in the subsequent digestive organs.

Furthermore, the temperature at which a protective film is formed on the core granules is 120 to 130°C when forming a protective film of only fatty acid salts, whereas the melting point temperature of fatty acids, oils, fats, waxes, etc. is 60 to 80°C.
The low temperature of ℃ not only prevents thermal decomposition of biologically active substances but also improves workability.

Furthermore, by using a protective substance that is itself insoluble in a neutral range and decomposable in an acidic range as a binder during the production of core granules, the rumen bypass properties of biologically active substances are improved. In particular, by using the same type of fatty acid salt as the protective coating, the bonding force between the core granules and the protective coating is strengthened, and as a result,
Destruction of the preparation due to rumination is also reduced and the first biologically active substance
Gastric bypass rates will further improve.

Furthermore, the titanium oxide-containing film of the outer layer not only blocks oxygen and prevents oxidation of the protective film, but also prevents deterioration due to light because the titanium oxide it contains blocks light. As a result, storage stability is improved.

〔Example〕

The present invention will be explained in more detail by Examples and Comparative Examples.

However, the scope of the present invention is not limited in any way by the following examples.

In addition, in the following steel, "parts" and "%" are based on weight unless otherwise specified.

(1) Preparation of core granules Calcium salt of beef tallow fatty acid with a melting point of 43°C
Heat it to 0°C to melt it, and add about 1 liter of methionine powder or risine hydrochloride powder to it.

After kneading for a minute, it was cooled to room temperature, crushed and sieved, and the particle size was 0.
．． Nuclear granules +A-1-A and -4 of 5 to 3 mm were prepared.

The composition and particle size of the obtained core granules are shown in Table 1.

(2) Preparation of coated preparation with protective film 1 kg of core granules A-1-A-4 prepared in the above item (1) was placed in a coating device (Henschel mixer) and heated to 65 to 70°C while stirring. heated to. Adding the same fatty acid calcium powder and hydrogenated beef tallow oil with a melting point of 60°C as used in item (1) above to this mixture, stirring is continued to form a protective film on the surface of the core granules to coat them. Then, a feed additive for ruminants was prepared.

The composition, coating amount, and biologically active substance content of the obtained feed additive for ruminants are shown in Table 1.

(3) Coating machine with titanium oxide-containing film (Hi-Coter, Freund Sangyo Co., Ltd.)
Coated preparation prepared in section (2) above and nuclear granules 2A-2 and A-4 prepared in section (1) (for comparison)
A titanium oxide-containing film was formed on 1 kg of each sample using a coating solution having the following composition to prepare a feed additive for ruminants.

[Coating liquid composition] Part by weight Titanium oxide powder 4.8 Hydroxypropyl cellulose 16.0 (HPC: 6 carbonate HPC-8L) Polyethylene glycol 1.6 (PEG:
Macrogol 6000) Water 84.
The composition of the titanium oxide-containing film of the obtained feed additive for ruminants is shown in Table 1.

(4) Dissolution test of biologically active substances 2 g each of the ruminant feed additive prepared in the above item (3) and the core granules prepared in the above item (1) for comparison
and Tris buffer 200c corresponding to bovine ruminal fluid.
After shaking and holding at a temperature of 37°C for 24 hours, it was taken out from the Tris buffer and 0.05M (=m) corresponding to bovine abomasal fluid.

A-dm-') It was immersed in 200 cc of hydrochloric acid and further shaken at a temperature of 37°C for 4 hours. Then, the preparation taken out from the 0.05 M hydrochloric acid was immersed in 200 cc of a solution corresponding to bovine small intestine, and further shaken at a temperature of 37°C for 4 hours.

Tris buffer 6.06 g of Tris [nidris(hydroxymethyl)aminomethane] was dissolved in 292 ml of 0.1 M hydrochloric acid, diluted to 1000 ml with water, pH 8.0, and then Tris buffer, 0.05 M Methionine and lysine eluted in hydrochloric acid and the corresponding small intestine fluid were quantified by iodine titration or ninhydrin color method. Quantification of vitamin E was performed by high performance liquid chromatography.

The test results are shown in Table 1.

(5) Storage stability test 2 g each of the ruminant feed additive prepared in the above item (3) and the core granules prepared in the above item (1) for comparison.
was left in direct sunlight for 80 days.

The POV and biologically active substance decomposition rate were measured for each sample after storage.

The measurement results are shown in Table 1 along with changes in appearance.

As shown in Table 2, the ruminant feed additive of the present invention shows no deterioration even when stored in a normal workplace, and has the same effect on ruminal fluid as when stored in a cool dark place. The elution rate of biologically active substances is extremely low (
, and most of the remainder is eluted in the fluid corresponding to the abomasum and the small intestine.

On the other hand, in formulations coated only with a titanium oxide-containing film and formulations without coating (see Comparative Examples), the dissolution rate of biologically active substances in the ruminal fluid is high.

In addition, in coated preparations with only a protective film without a titanium oxide film, not only deterioration is observed in appearance, but also
POV increases significantly and biologically active substances are also degraded.

〔Effect of the invention〕

As shown in the examples above, the feed additive for ruminants of the present invention not only has excellent ruminal bypass properties for biologically active substances contained therein when orally administered to ruminants. It also has excellent storage stability.

The present invention provides biologically active substances that are easily degraded in the rumen of ruminants when administered orally, and which are suitable for bypassing the rumen and absorbing them with high efficiency in the digestive organs from the abomasum onwards.
The present invention provides a feed additive for ruminants with excellent storage stability, and has extremely great significance in industry, particularly in the livestock field.

Claims (6)

[Claims] (1) Core granules containing biologically active substances and protective substances are selected from the group consisting of (a) fatty acid salts that are insoluble in neutral ranges and degradable in acidic ranges, and (b) oils, fats, and waxes. A feed additive for ruminants characterized by being coated with two layers: a protective film consisting of at least one selected species and a film containing titanium oxide. (2) A feed additive for ruminants according to claim (1), wherein the biologically active substance is at least one selected from the group consisting of methionine, lysine hydrochloride, and vitamins. (3) The feed additive for ruminants according to claim (1), wherein the protective substance is a fatty acid salt of the same type as the fatty acid salt that is a component of the protective film. (4) The feed additive for ruminants according to claim (1), wherein the fatty acid salt as a component of the protective film is a calcium salt of mixed fatty acids having a melting point in the range of 40 to 50°C. (5) The feed additive for ruminants according to claim (1), wherein the titanium oxide-containing film is a water-soluble polymer film containing titanium oxide powder, flakes, etc. dispersed therein. (6) The feed additive for ruminants according to claim (4), wherein the water-soluble polymer is a water-soluble cellulose derivative.