JPH0441996B2 - - Google Patents

Info

Publication number: JPH0441996B2
Authority: JP; Japan
Prior art keywords: megadaltons; restriction enzyme; dna; cleavage; plasmid
Prior art date: 1983-04-28
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP7556083A

Other languages

English (en)

Japanese (ja)

Other versions

JPS59210887A (ja

Inventor

Takanosuke Sano

Koichi Ito

Kyoshi Miwa

Shigeru Nakamori

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Ajinomoto Co Inc

Original Assignee

Ajinomoto Co Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-04-28

Filing date

1983-04-28

Publication date

1992-07-10

1983-04-28 Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc

1983-04-28 Priority to JP7556083A priority Critical patent/JPS59210887A/ja

1984-11-29 Publication of JPS59210887A publication Critical patent/JPS59210887A/ja

1992-07-10 Publication of JPH0441996B2 publication Critical patent/JPH0441996B2/ja

Status Granted legal-status Critical Current

Links

239000013612 plasmid Substances 0.000 claims description 89
108091008146 restriction endonucleases Proteins 0.000 claims description 60
238000003776 cleavage reaction Methods 0.000 claims description 45
230000007017 scission Effects 0.000 claims description 44
239000012634 fragment Substances 0.000 claims description 35
241000186146 Brevibacterium Species 0.000 claims description 23
241000894006 Bacteria Species 0.000 claims description 22
108010082690 diaminopimelic acid decarboxylase Proteins 0.000 claims description 13
108030003594 Diaminopimelate decarboxylases Proteins 0.000 claims description 10
239000013611 chromosomal DNA Substances 0.000 claims description 9
GMKMEZVLHJARHF-SYDPRGILSA-N meso-2,6-diaminopimelic acid Chemical compound [O-]C(=O)[C@@H]([NH3+])CCC[C@@H]([NH3+])C([O-])=O GMKMEZVLHJARHF-SYDPRGILSA-N 0.000 claims description 9
244000005700 microbiome Species 0.000 claims description 9
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 8
230000000694 effects Effects 0.000 claims description 8
238000005520 cutting process Methods 0.000 claims description 6
229930195712 glutamate Natural products 0.000 claims description 6
108090000623 proteins and genes Proteins 0.000 claims description 6
102000004031 Carboxy-Lyases Human genes 0.000 claims description 5
238000002360 preparation method Methods 0.000 claims description 5
210000000349 chromosome Anatomy 0.000 claims description 4
239000004220 glutamic acid Substances 0.000 claims description 4
230000002950 deficient Effects 0.000 claims description 3
101001058938 Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / CCUG 9996 / CIP 106432 / VR4) Glutaconyl-CoA decarboxylase subunit beta Proteins 0.000 claims 4
108020004414 DNA Proteins 0.000 description 56
238000000034 method Methods 0.000 description 26
210000004027 cell Anatomy 0.000 description 24
KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 20
210000001938 protoplast Anatomy 0.000 description 20
241000186226 Corynebacterium glutamicum Species 0.000 description 17
239000004472 Lysine Substances 0.000 description 16
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 14
239000002131 composite material Substances 0.000 description 13
235000018977 lysine Nutrition 0.000 description 13
238000006243 chemical reaction Methods 0.000 description 11
229960005091 chloramphenicol Drugs 0.000 description 11
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 11
239000000243 solution Substances 0.000 description 10
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
230000001580 bacterial effect Effects 0.000 description 9
238000004519 manufacturing process Methods 0.000 description 9
241000588724 Escherichia coli Species 0.000 description 8
238000000246 agarose gel electrophoresis Methods 0.000 description 8
230000009466 transformation Effects 0.000 description 8
238000002955 isolation Methods 0.000 description 7
GMKMEZVLHJARHF-UHFFFAOYSA-N (2R,6R)-form-2.6-Diaminoheptanedioic acid Natural products OC(=O)C(N)CCCC(N)C(O)=O GMKMEZVLHJARHF-UHFFFAOYSA-N 0.000 description 6
108090000790 Enzymes Proteins 0.000 description 6
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 6
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
238000005119 centrifugation Methods 0.000 description 6
229930027917 kanamycin Natural products 0.000 description 6
229960000318 kanamycin Drugs 0.000 description 6
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 6
229930182823 kanamycin A Natural products 0.000 description 6
229920001817 Agar Polymers 0.000 description 5
101000798402 Bacillus licheniformis Ornithine racemase Proteins 0.000 description 5
244000063299 Bacillus subtilis Species 0.000 description 5
235000014469 Bacillus subtilis Nutrition 0.000 description 5
102000004190 Enzymes Human genes 0.000 description 5
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
102000016943 Muramidase Human genes 0.000 description 5
108010014251 Muramidase Proteins 0.000 description 5
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 5
239000008272 agar Substances 0.000 description 5
230000003698 anagen phase Effects 0.000 description 5
238000012258 culturing Methods 0.000 description 5
229940088598 enzyme Drugs 0.000 description 5
238000002474 experimental method Methods 0.000 description 5
239000008103 glucose Substances 0.000 description 5
229960000274 lysozyme Drugs 0.000 description 5
239000004325 lysozyme Substances 0.000 description 5
235000010335 lysozyme Nutrition 0.000 description 5
239000003550 marker Substances 0.000 description 5
230000008929 regeneration Effects 0.000 description 5
238000011069 regeneration method Methods 0.000 description 5
239000006228 supernatant Substances 0.000 description 5
AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 4
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 4
239000001888 Peptone Substances 0.000 description 4
108010080698 Peptones Proteins 0.000 description 4
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
230000003115 biocidal effect Effects 0.000 description 4
229940041514 candida albicans extract Drugs 0.000 description 4
238000001962 electrophoresis Methods 0.000 description 4
239000006166 lysate Substances 0.000 description 4
230000002934 lysing effect Effects 0.000 description 4
235000019319 peptone Nutrition 0.000 description 4
239000012138 yeast extract Substances 0.000 description 4
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
241000186216 Corynebacterium Species 0.000 description 3
102000012410 DNA Ligases Human genes 0.000 description 3
108010061982 DNA Ligases Proteins 0.000 description 3
235000019766 L-Lysine Nutrition 0.000 description 3
201000004283 Shwachman-Diamond syndrome Diseases 0.000 description 3
239000002253 acid Substances 0.000 description 3
238000004166 bioassay Methods 0.000 description 3
239000001110 calcium chloride Substances 0.000 description 3
229910001628 calcium chloride Inorganic materials 0.000 description 3
235000011148 calcium chloride Nutrition 0.000 description 3
238000010276 construction Methods 0.000 description 3
239000000499 gel Substances 0.000 description 3
229910001629 magnesium chloride Inorganic materials 0.000 description 3
238000013508 migration Methods 0.000 description 3
230000005012 migration Effects 0.000 description 3
239000000203 mixture Substances 0.000 description 3
230000000644 propagated effect Effects 0.000 description 3
238000011426 transformation method Methods 0.000 description 3
241001485655 Corynebacterium glutamicum ATCC 13032 Species 0.000 description 2
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
239000002202 Polyethylene glycol Substances 0.000 description 2
101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 2
102000006382 Ribonucleases Human genes 0.000 description 2
108010083644 Ribonucleases Proteins 0.000 description 2
239000004473 Threonine Substances 0.000 description 2
239000011543 agarose gel Substances 0.000 description 2
239000003242 anti bacterial agent Substances 0.000 description 2
239000000872 buffer Substances 0.000 description 2
239000012153 distilled water Substances 0.000 description 2
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
238000001493 electron microscopy Methods 0.000 description 2
235000013922 glutamic acid Nutrition 0.000 description 2
238000010438 heat treatment Methods 0.000 description 2
229940056360 penicillin g Drugs 0.000 description 2
229920001223 polyethylene glycol Polymers 0.000 description 2
238000011160 research Methods 0.000 description 2
GHSJKUNUIHUPDF-UHFFFAOYSA-N s-(2-aminoethyl)-l-cysteine Chemical compound NCCSCC(N)C(O)=O GHSJKUNUIHUPDF-UHFFFAOYSA-N 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
229940074404 sodium succinate Drugs 0.000 description 2
ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 description 2
229960002898 threonine Drugs 0.000 description 2
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
238000005406 washing Methods 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 description 1
241000186361 Actinobacteria <class> Species 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
241000304886 Bacilli Species 0.000 description 1
241000193830 Bacillus <bacterium> Species 0.000 description 1
108090000489 Carboxy-Lyases Proteins 0.000 description 1
241001517047 Corynebacterium acetoacidophilum Species 0.000 description 1
241000186145 Corynebacterium ammoniagenes Species 0.000 description 1
FFEARJCKVFRZRR-SCSAIBSYSA-N D-methionine Chemical compound CSCC[C@@H](N)C(O)=O FFEARJCKVFRZRR-SCSAIBSYSA-N 0.000 description 1
102000053602 DNA Human genes 0.000 description 1
108010069941 DNA receptor Proteins 0.000 description 1
206010059866 Drug resistance Diseases 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
241001646716 Escherichia coli K-12 Species 0.000 description 1
GRSZFWQUAKGDAV-KQYNXXCUSA-L IMP(2-) Chemical compound O[C@@H]1[C@H](O)[C@@H](COP([O-])([O-])=O)O[C@H]1N1C(N=CNC2=O)=C2N=C1 GRSZFWQUAKGDAV-KQYNXXCUSA-L 0.000 description 1
UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
229930010555 Inosine Natural products 0.000 description 1
ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
241000144155 Microbacterium ammoniaphilum Species 0.000 description 1
OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
241000187561 Rhodococcus erythropolis Species 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
241000191967 Staphylococcus aureus Species 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
239000004098 Tetracycline Substances 0.000 description 1
AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
241000700605 Viruses Species 0.000 description 1
241000319304 [Brevibacterium] flavum Species 0.000 description 1
239000008351 acetate buffer Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
235000001014 amino acid Nutrition 0.000 description 1
229940024606 amino acid Drugs 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
229960000723 ampicillin Drugs 0.000 description 1
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
239000005557 antagonist Substances 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
238000003556 assay Methods 0.000 description 1
AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
238000010367 cloning Methods 0.000 description 1
238000012217 deletion Methods 0.000 description 1
230000037430 deletion Effects 0.000 description 1
238000000432 density-gradient centrifugation Methods 0.000 description 1
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
235000019797 dipotassium phosphate Nutrition 0.000 description 1
229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
239000003814 drug Substances 0.000 description 1
229940079593 drug Drugs 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
238000012869 ethanol precipitation Methods 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
229960005542 ethidium bromide Drugs 0.000 description 1
238000000605 extraction Methods 0.000 description 1
238000012215 gene cloning Methods 0.000 description 1
230000012010 growth Effects 0.000 description 1
229960003786 inosine Drugs 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
239000007788 liquid Substances 0.000 description 1
238000004811 liquid chromatography Methods 0.000 description 1
229960003646 lysine Drugs 0.000 description 1
VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
239000011976 maleic acid Substances 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
229960004452 methionine Drugs 0.000 description 1
238000002156 mixing Methods 0.000 description 1
239000000178 monomer Substances 0.000 description 1
230000035699 permeability Effects 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
239000000047 product Substances 0.000 description 1
230000035755 proliferation Effects 0.000 description 1
239000002212 purine nucleoside Substances 0.000 description 1
239000002213 purine nucleotide Substances 0.000 description 1
150000003212 purines Chemical class 0.000 description 1
238000011084 recovery Methods 0.000 description 1
239000000126 substance Substances 0.000 description 1
239000000758 substrate Substances 0.000 description 1
WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
229960002180 tetracycline Drugs 0.000 description 1
229930101283 tetracycline Natural products 0.000 description 1
235000019364 tetracycline Nutrition 0.000 description 1
150000003522 tetracyclines Chemical class 0.000 description 1
VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
238000005199 ultracentrifugation Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

JP7556083A 1983-04-28 1983-04-28 Ｄｎａ断片及びその調製法 Granted JPS59210887A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP7556083A JPS59210887A (ja)	1983-04-28	1983-04-28	Ｄｎａ断片及びその調製法

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP7556083A JPS59210887A (ja)	1983-04-28	1983-04-28	Ｄｎａ断片及びその調製法

Publications (2)

Publication Number	Publication Date
JPS59210887A JPS59210887A (ja)	1984-11-29
JPH0441996B2 true JPH0441996B2 (de)	1992-07-10

Family

ID=13579681

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP7556083A Granted JPS59210887A (ja)	1983-04-28	1983-04-28	Ｄｎａ断片及びその調製法

Country Status (1)

Country	Link
JP (1)	JPS59210887A (de)

1983
- 1983-04-28 JP JP7556083A patent/JPS59210887A/ja active Granted

Also Published As

Publication number	Publication date
JPS59210887A (ja)	1984-11-29

Publication	Publication Date	Title
US4514502A (en)	1985-04-30	Composite plasmid
JP2973446B2 (ja)	1999-11-08	新規プラスミドベクター
EP0077548B1 (de)	1989-01-18	Plasmide
JPH06102024B2 (ja)	1994-12-14	新規プロモーター及び該プロモーターを用いた遺伝子発現方法
JPH0691827B2 (ja)	1994-11-16	新規ベクタ−プラスミド
US4861722A (en)	1989-08-29	Coryneform bacteria carrying recombinant plasmids and their use in the fermentative production of L-lysine
JP3979679B2 (ja)	2007-09-19	プロモーター機能を有するｄｎａ断片
EP0093611B2 (de)	1995-03-22	Mehrkomponentenplasmid
EP0137348B1 (de)	1991-12-04	Rekombinante DNS, diese rekombinante DNS enthaltende Bakterien, und Verfahren zur Herstellung von L-Threonin oder L-Isoleucin unter Verwendung dieser Bakterien
EP0158940B1 (de)	1991-12-11	Zusammengesetztes transduzierbares Plasmid
EP0124048B1 (de)	1992-07-22	Verfahren zur Herstellung von L-Tryptophan durch Gärung
US5426050A (en)	1995-06-20	Plasmid vectors for expression of genes in coryneform bacteria
EP0215388B1 (de)	1991-12-04	Plasmidvektor und ein Verfahren zur Regulierung der Genexpression unter Verwendung dieses Vektors
EP0137387B1 (de)	1991-09-04	Verfahren zur Herstellung von L-Phenylalanin durch Fermentation
JPH0441996B2 (de)	1992-07-10
US4778762A (en)	1988-10-18	Plasmid
EP0170257B1 (de)	1992-04-15	Verfahren zur Herstellung von L-Tryptophan
JPH05184371A (ja)	1993-07-27	ジヒドロジピコリン酸シンセターゼをコードする遺伝子ｄｎａ及びその利用
JPH0616713B2 (ja)	1994-03-09	複合プラスミド
JPH0559710B2 (de)	1993-08-31
JPH0321154B2 (de)	1991-03-22
JPH0734744B2 (ja)	1995-04-19	形質導入可能な複合プラスミド
JPH0734745B2 (ja)	1995-04-19	形質導入方法
JPH0476678B2 (de)	1992-12-04
JPH055479B2 (de)	1993-01-22