JPH047759B2 - - Google Patents

Info

Publication number: JPH047759B2
Authority: JP; Japan
Prior art keywords: water; antibody protein; antibody; aqueous phase; monolayer
Prior art date: 1986-08-25
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP19722086A

Other languages

English (en)

Japanese (ja)

Other versions

JPS6354399A (ja

Inventor

Takeyuki Kawaguchi

Hisashi Jo

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Teijin Ltd

Original Assignee

Teijin Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-08-25

Filing date

1986-08-25

Publication date

1992-02-12

1986-08-25 Application filed by Teijin Ltd filed Critical Teijin Ltd

1986-08-25 Priority to JP19722086A priority Critical patent/JPS6354399A/ja

1988-03-08 Publication of JPS6354399A publication Critical patent/JPS6354399A/ja

1992-02-12 Publication of JPH047759B2 publication Critical patent/JPH047759B2/ja

Status Granted legal-status Critical Current

Links

102000004169 proteins and genes Human genes 0.000 claims description 30
108090000623 proteins and genes Proteins 0.000 claims description 30
238000000034 method Methods 0.000 claims description 14
239000000758 substrate Substances 0.000 claims description 11
239000007787 solid Substances 0.000 claims description 10
239000008346 aqueous phase Substances 0.000 claims description 7
230000003100 immobilizing effect Effects 0.000 claims description 7
239000002356 single layer Substances 0.000 claims description 6
150000002148 esters Chemical class 0.000 claims description 5
150000004668 long chain fatty acids Chemical class 0.000 claims description 4
229910052751 metal Inorganic materials 0.000 claims description 4
239000002184 metal Substances 0.000 claims description 4
150000003839 salts Chemical class 0.000 claims description 4
125000004432 carbon atom Chemical group C* 0.000 claims description 3
239000013554 lipid monolayer Substances 0.000 claims description 3
230000005501 phase interface Effects 0.000 claims description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 23
239000012528 membrane Substances 0.000 description 12
238000006243 chemical reaction Methods 0.000 description 9
230000000694 effects Effects 0.000 description 9
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 7
239000000243 solution Substances 0.000 description 6
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
102000036639 antigens Human genes 0.000 description 4
108091007433 antigens Proteins 0.000 description 4
150000002632 lipids Chemical class 0.000 description 4
KILNVBDSWZSGLL-KXQOOQHDSA-N 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCCCCCCCCC KILNVBDSWZSGLL-KXQOOQHDSA-N 0.000 description 3
UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
235000021353 Lignoceric acid Nutrition 0.000 description 3
CQXMAMUUWHYSIY-UHFFFAOYSA-N Lignoceric acid Natural products CCCCCCCCCCCCCCCCCCCCCCCC(=O)OCCC1=CC=C(O)C=C1 CQXMAMUUWHYSIY-UHFFFAOYSA-N 0.000 description 3
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
235000021355 Stearic acid Nutrition 0.000 description 3
239000002253 acid Substances 0.000 description 3
239000000427 antigen Substances 0.000 description 3
239000007864 aqueous solution Substances 0.000 description 3
230000001186 cumulative effect Effects 0.000 description 3
FARYTWBWLZAXNK-WAYWQWQTSA-N ethyl (z)-3-(methylamino)but-2-enoate Chemical compound CCOC(=O)\C=C(\C)NC FARYTWBWLZAXNK-WAYWQWQTSA-N 0.000 description 3
239000010410 layer Substances 0.000 description 3
QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 3
OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 3
VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
230000007480 spreading Effects 0.000 description 3
239000008117 stearic acid Substances 0.000 description 3
108010022366 Carcinoembryonic Antigen Proteins 0.000 description 2
102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 description 2
102000004190 Enzymes Human genes 0.000 description 2
108090000790 Enzymes Proteins 0.000 description 2
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
238000000862 absorption spectrum Methods 0.000 description 2
238000009825 accumulation Methods 0.000 description 2
VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 2
230000000890 antigenic effect Effects 0.000 description 2
235000014113 dietary fatty acids Nutrition 0.000 description 2
229940088598 enzyme Drugs 0.000 description 2
229930195729 fatty acid Natural products 0.000 description 2
239000000194 fatty acid Substances 0.000 description 2
150000004665 fatty acids Chemical class 0.000 description 2
XMHIUKTWLZUKEX-UHFFFAOYSA-N hexacosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCCCCCCCC(O)=O XMHIUKTWLZUKEX-UHFFFAOYSA-N 0.000 description 2
230000002209 hydrophobic effect Effects 0.000 description 2
VKOBVWXKNCXXDE-UHFFFAOYSA-N icosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCC(O)=O VKOBVWXKNCXXDE-UHFFFAOYSA-N 0.000 description 2
229940027941 immunoglobulin g Drugs 0.000 description 2
UTOPWMOLSKOLTQ-UHFFFAOYSA-N octacosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCCCCCCCCCC(O)=O UTOPWMOLSKOLTQ-UHFFFAOYSA-N 0.000 description 2
238000005192 partition Methods 0.000 description 2
GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
102000016938 Catalase Human genes 0.000 description 1
108010053835 Catalase Proteins 0.000 description 1
102000011022 Chorionic Gonadotropin Human genes 0.000 description 1
108010062540 Chorionic Gonadotropin Proteins 0.000 description 1
102000008857 Ferritin Human genes 0.000 description 1
108050000784 Ferritin Proteins 0.000 description 1
238000008416 Ferritin Methods 0.000 description 1
108010015776 Glucose oxidase Proteins 0.000 description 1
239000004366 Glucose oxidase Substances 0.000 description 1
206010029719 Nonspecific reaction Diseases 0.000 description 1
102000003992 Peroxidases Human genes 0.000 description 1
BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
JAWMENYCRQKKJY-UHFFFAOYSA-N [3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-ylmethyl)-1-oxa-2,8-diazaspiro[4.5]dec-2-en-8-yl]-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]methanone Chemical compound N1N=NC=2CN(CCC=21)CC1=NOC2(C1)CCN(CC2)C(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F JAWMENYCRQKKJY-UHFFFAOYSA-N 0.000 description 1
229910052784 alkaline earth metal Inorganic materials 0.000 description 1
150000001342 alkaline earth metals Chemical class 0.000 description 1
229910052782 aluminium Inorganic materials 0.000 description 1
XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
239000003012 bilayer membrane Substances 0.000 description 1
229910052793 cadmium Inorganic materials 0.000 description 1
BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
150000001735 carboxylic acids Chemical class 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
229940015047 chorionic gonadotropin Drugs 0.000 description 1
230000000052 comparative effect Effects 0.000 description 1
238000010668 complexation reaction Methods 0.000 description 1
239000002131 composite material Substances 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
230000006835 compression Effects 0.000 description 1
238000007906 compression Methods 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
230000009849 deactivation Effects 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000036425 denaturation Effects 0.000 description 1
238000012631 diagnostic technique Methods 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
125000000524 functional group Chemical group 0.000 description 1
229940116332 glucose oxidase Drugs 0.000 description 1
235000019420 glucose oxidase Nutrition 0.000 description 1
238000007654 immersion Methods 0.000 description 1
230000005965 immune activity Effects 0.000 description 1
230000003993 interaction Effects 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
239000000463 material Substances 0.000 description 1
238000005259 measurement Methods 0.000 description 1
229910021645 metal ion Inorganic materials 0.000 description 1
239000011259 mixed solution Substances 0.000 description 1
239000003960 organic solvent Substances 0.000 description 1
108040007629 peroxidase activity proteins Proteins 0.000 description 1
239000010453 quartz Substances 0.000 description 1
230000004044 response Effects 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
229910000077 silane Inorganic materials 0.000 description 1
238000012546 transfer Methods 0.000 description 1

Landscapes

Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Peptides Or Proteins (AREA)

JP19722086A 1986-08-25 1986-08-25 脂質単分子膜を用いた抗体タンパクの固定化方法 Granted JPS6354399A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP19722086A JPS6354399A (ja)	1986-08-25	1986-08-25	脂質単分子膜を用いた抗体タンパクの固定化方法

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP19722086A JPS6354399A (ja)	1986-08-25	1986-08-25	脂質単分子膜を用いた抗体タンパクの固定化方法

Publications (2)

Publication Number	Publication Date
JPS6354399A JPS6354399A (ja)	1988-03-08
JPH047759B2 true JPH047759B2 (da)	1992-02-12

Family

ID=16370833

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP19722086A Granted JPS6354399A (ja)	1986-08-25	1986-08-25	脂質単分子膜を用いた抗体タンパクの固定化方法

Country Status (1)

Country	Link
JP (1)	JPS6354399A (da)

1986
- 1986-08-25 JP JP19722086A patent/JPS6354399A/ja active Granted

Also Published As

Publication number	Publication date
JPS6354399A (ja)	1988-03-08

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