JPH047760B2 - - Google Patents

Info

Publication number: JPH047760B2
Authority: JP; Japan
Prior art keywords: antibody protein; antibody; water; monolayer; aqueous phase
Prior art date: 1986-08-25
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP19722186A

Other languages

English (en)

Japanese (ja)

Other versions

JPS6354400A (ja

Inventor

Takeyuki Kawaguchi

Hisashi Jo

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Teijin Ltd

Original Assignee

Teijin Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-08-25

Filing date

1986-08-25

Publication date

1992-02-12

1986-08-25 Application filed by Teijin Ltd filed Critical Teijin Ltd

1986-08-25 Priority to JP19722186A priority Critical patent/JPS6354400A/ja

1988-03-08 Publication of JPS6354400A publication Critical patent/JPS6354400A/ja

1992-02-12 Publication of JPH047760B2 publication Critical patent/JPH047760B2/ja

Status Granted legal-status Critical Current

Links

102000004169 proteins and genes Human genes 0.000 claims description 30
108090000623 proteins and genes Proteins 0.000 claims description 30
238000000034 method Methods 0.000 claims description 11
239000000758 substrate Substances 0.000 claims description 11
239000007787 solid Substances 0.000 claims description 10
230000003100 immobilizing effect Effects 0.000 claims description 8
239000008346 aqueous phase Substances 0.000 claims description 6
239000002356 single layer Substances 0.000 claims description 6
150000002148 esters Chemical class 0.000 claims description 5
239000013554 lipid monolayer Substances 0.000 claims description 4
229910052751 metal Inorganic materials 0.000 claims description 4
239000002184 metal Substances 0.000 claims description 4
150000003839 salts Chemical class 0.000 claims description 4
125000004432 carbon atom Chemical group C* 0.000 claims description 3
150000004668 long chain fatty acids Chemical class 0.000 claims description 3
230000005501 phase interface Effects 0.000 claims description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 20
239000012528 membrane Substances 0.000 description 10
238000006243 chemical reaction Methods 0.000 description 9
230000000694 effects Effects 0.000 description 8
235000021355 Stearic acid Nutrition 0.000 description 7
QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 7
OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 7
239000008117 stearic acid Substances 0.000 description 7
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 6
102000036639 antigens Human genes 0.000 description 5
108091007433 antigens Proteins 0.000 description 5
239000007864 aqueous solution Substances 0.000 description 5
239000000243 solution Substances 0.000 description 5
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
239000000427 antigen Substances 0.000 description 4
IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 4
150000002632 lipids Chemical class 0.000 description 4
KILNVBDSWZSGLL-KXQOOQHDSA-N 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCCCCCCCCC KILNVBDSWZSGLL-KXQOOQHDSA-N 0.000 description 3
UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 description 3
229910001626 barium chloride Inorganic materials 0.000 description 3
239000012153 distilled water Substances 0.000 description 3
239000010410 layer Substances 0.000 description 3
VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
230000007480 spreading Effects 0.000 description 3
108010022366 Carcinoembryonic Antigen Proteins 0.000 description 2
102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 description 2
102000004190 Enzymes Human genes 0.000 description 2
108090000790 Enzymes Proteins 0.000 description 2
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
235000021314 Palmitic acid Nutrition 0.000 description 2
VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 2
229910052793 cadmium Inorganic materials 0.000 description 2
BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 2
230000001186 cumulative effect Effects 0.000 description 2
235000014113 dietary fatty acids Nutrition 0.000 description 2
UKMSUNONTOPOIO-UHFFFAOYSA-N docosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCCCC(O)=O UKMSUNONTOPOIO-UHFFFAOYSA-N 0.000 description 2
229940088598 enzyme Drugs 0.000 description 2
229930195729 fatty acid Natural products 0.000 description 2
239000000194 fatty acid Substances 0.000 description 2
150000004665 fatty acids Chemical class 0.000 description 2
VKOBVWXKNCXXDE-UHFFFAOYSA-N icosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCC(O)=O VKOBVWXKNCXXDE-UHFFFAOYSA-N 0.000 description 2
229940027941 immunoglobulin g Drugs 0.000 description 2
WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 2
238000005192 partition Methods 0.000 description 2
229910000028 potassium bicarbonate Inorganic materials 0.000 description 2
235000015497 potassium bicarbonate Nutrition 0.000 description 2
239000011736 potassium bicarbonate Substances 0.000 description 2
TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 2
229940086066 potassium hydrogencarbonate Drugs 0.000 description 2
239000010453 quartz Substances 0.000 description 2
GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
TWJNQYPJQDRXPH-UHFFFAOYSA-N 2-cyanobenzohydrazide Chemical compound NNC(=O)C1=CC=CC=C1C#N TWJNQYPJQDRXPH-UHFFFAOYSA-N 0.000 description 1
235000021357 Behenic acid Nutrition 0.000 description 1
102000016938 Catalase Human genes 0.000 description 1
108010053835 Catalase Proteins 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
102000011022 Chorionic Gonadotropin Human genes 0.000 description 1
108010062540 Chorionic Gonadotropin Proteins 0.000 description 1
102000008857 Ferritin Human genes 0.000 description 1
108050000784 Ferritin Proteins 0.000 description 1
238000008416 Ferritin Methods 0.000 description 1
108010015776 Glucose oxidase Proteins 0.000 description 1
239000004366 Glucose oxidase Substances 0.000 description 1
235000021360 Myristic acid Nutrition 0.000 description 1
TUNFSRHWOTWDNC-UHFFFAOYSA-N Myristic acid Natural products CCCCCCCCCCCCCC(O)=O TUNFSRHWOTWDNC-UHFFFAOYSA-N 0.000 description 1
VCUFZILGIRCDQQ-KRWDZBQOSA-N N-[[(5S)-2-oxo-3-(2-oxo-3H-1,3-benzoxazol-6-yl)-1,3-oxazolidin-5-yl]methyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C1O[C@H](CN1C1=CC2=C(NC(O2)=O)C=C1)CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F VCUFZILGIRCDQQ-KRWDZBQOSA-N 0.000 description 1
206010029719 Nonspecific reaction Diseases 0.000 description 1
102000003992 Peroxidases Human genes 0.000 description 1
BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
238000004847 absorption spectroscopy Methods 0.000 description 1
238000000862 absorption spectrum Methods 0.000 description 1
239000002253 acid Substances 0.000 description 1
229910052784 alkaline earth metal Inorganic materials 0.000 description 1
150000001342 alkaline earth metals Chemical class 0.000 description 1
229910052782 aluminium Inorganic materials 0.000 description 1
XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
230000000890 antigenic effect Effects 0.000 description 1
229940116226 behenic acid Drugs 0.000 description 1
239000003012 bilayer membrane Substances 0.000 description 1
150000001732 carboxylic acid derivatives Chemical class 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
229940015047 chorionic gonadotropin Drugs 0.000 description 1
230000000052 comparative effect Effects 0.000 description 1
238000010668 complexation reaction Methods 0.000 description 1
239000002131 composite material Substances 0.000 description 1
230000006835 compression Effects 0.000 description 1
238000007906 compression Methods 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
230000009849 deactivation Effects 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000036425 denaturation Effects 0.000 description 1
238000012631 diagnostic technique Methods 0.000 description 1
238000007598 dipping method Methods 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
125000000524 functional group Chemical group 0.000 description 1
229940116332 glucose oxidase Drugs 0.000 description 1
235000019420 glucose oxidase Nutrition 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
230000005965 immune activity Effects 0.000 description 1
230000003993 interaction Effects 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
239000000463 material Substances 0.000 description 1
238000005259 measurement Methods 0.000 description 1
229910021645 metal ion Inorganic materials 0.000 description 1
239000011259 mixed solution Substances 0.000 description 1
239000003960 organic solvent Substances 0.000 description 1
108040007629 peroxidase activity proteins Proteins 0.000 description 1
230000004044 response Effects 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
229910000077 silane Inorganic materials 0.000 description 1
238000012546 transfer Methods 0.000 description 1

Landscapes

Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Peptides Or Proteins (AREA)

JP19722186A 1986-08-25 1986-08-25 脂質単分子膜により抗体タンパクを固定化する方法 Granted JPS6354400A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP19722186A JPS6354400A (ja)	1986-08-25	1986-08-25	脂質単分子膜により抗体タンパクを固定化する方法

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP19722186A JPS6354400A (ja)	1986-08-25	1986-08-25	脂質単分子膜により抗体タンパクを固定化する方法

Publications (2)

Publication Number	Publication Date
JPS6354400A JPS6354400A (ja)	1988-03-08
JPH047760B2 true JPH047760B2 (de)	1992-02-12

Family

ID=16370848

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP19722186A Granted JPS6354400A (ja)	1986-08-25	1986-08-25	脂質単分子膜により抗体タンパクを固定化する方法

Country Status (1)

Country	Link
JP (1)	JPS6354400A (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1991003589A1 (en) *	1989-09-07	1991-03-21	Matsushita Electric Industrial Co., Ltd.	Washing machine
JP5891951B2 (ja) *	2012-05-28	2016-03-23	トヨタ紡織株式会社	車両用シート

1986
- 1986-08-25 JP JP19722186A patent/JPS6354400A/ja active Granted

Also Published As

Publication number	Publication date
JPS6354400A (ja)	1988-03-08

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JP3713516B2 (ja)	2005-11-09	ポリマー変態を基礎とするセンサ
US5405766A (en)	1995-04-11	Immobilization of biologically active protein on a support with a 7-18 carbon spacer and a bifunctional phospholipid
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US6241863B1 (en)	2001-06-05	Amperometric biosensors based on redox enzymes
JPH08505123A (ja)	1996-06-04	改良されたセンサー膜
JPH04501605A (ja)	1992-03-19	バイオセンサーシステムに使用する選択的生体分子相互作用の可能な検出表面
JPH03505222A (ja)	1991-11-14	担体にたん白質、ペプチド、補酵素等々を固定化する方法
WO1986004095A1 (en)	1986-07-17	Preparation of reagents
JPH0561279B2 (de)	1993-09-06
JPH047760B2 (de)	1992-02-12
Rodrigues et al.	2014	Cellulase and alcohol dehydrogenase immobilized in Langmuir and Langmuir–Blodgett films and their molecular-level effects upon contact with cellulose and ethanol
US6316273B1 (en)	2001-11-13	Biosensor for detection of small molecule analytes
US5879878A (en)	1999-03-09	Method of producing a first layer electrode membrane for a biosensor
JPH047759B2 (de)	1992-02-12
FR2550799A1 (fr)	1985-02-22	Compose marque par une enzyme, son procede de preparation et son utilisation en enzymoimmunologie
JPS60155129A (ja)	1985-08-15	固定化抗原または抗体の製造方法
JPH0473749B2 (de)	1992-11-24
JPH0285755A (ja)	1990-03-27	免疫センサ
Damrongchai et al.	1995	Calcium responsive two-dimensional molecular assembling of lipid-conjugated calmodulin
Gabrielli et al.	2000	Interactions and reactions of monolayers and Langmuir–Blodgett multilayers with compounds in the bulk phase
JPH02272081A (ja)	1990-11-06	機能性有機薄膜
JP3509142B2 (ja)	2004-03-22	反応性リポソームおよび形成剤
AU692107B2 (en)	1998-05-28	Detection of small analytes
Brennan et al.	1994	Transduction of the reaction between urea and covalently immobilized urease by fluorescent amphiphilic membranes