JPH0529040B2 - - Google Patents

Info

Publication number: JPH0529040B2
Authority: JP; Japan
Prior art keywords: protein; chlorophyll; gel; surfactant; electrophoresis
Prior art date: 1985-07-24
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

JP60163230A

Other languages

English (en)

Japanese (ja)

Other versions

JPS6222796A (ja

Inventor

Kanenobu Kubo

Masahiro Fukuda

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Lion Corp

Original Assignee

Lion Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-07-24

Filing date

1985-07-24

Publication date

1993-04-28

1985-07-24 Application filed by Lion Corp filed Critical Lion Corp

1985-07-24 Priority to JP60163230A priority Critical patent/JPS6222796A/ja

1987-01-30 Publication of JPS6222796A publication Critical patent/JPS6222796A/ja

1993-04-28 Publication of JPH0529040B2 publication Critical patent/JPH0529040B2/ja

Status Granted legal-status Critical Current

Links

238000000034 method Methods 0.000 description 28
108010052285 Membrane Proteins Proteins 0.000 description 25
239000004094 surface-active agent Substances 0.000 description 21
239000000499 gel Substances 0.000 description 20
102000004169 proteins and genes Human genes 0.000 description 20
108090000623 proteins and genes Proteins 0.000 description 20
235000018102 proteins Nutrition 0.000 description 17
238000000746 purification Methods 0.000 description 17
238000000926 separation method Methods 0.000 description 15
239000003945 anionic surfactant Substances 0.000 description 14
239000000872 buffer Substances 0.000 description 14
239000007864 aqueous solution Substances 0.000 description 13
ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 13
238000001962 electrophoresis Methods 0.000 description 13
229930002875 chlorophyll Natural products 0.000 description 12
235000019804 chlorophyll Nutrition 0.000 description 12
238000001502 gel electrophoresis Methods 0.000 description 11
239000000975 dye Substances 0.000 description 9
229920002401 polyacrylamide Polymers 0.000 description 9
125000004432 carbon atom Chemical group C* 0.000 description 8
239000000693 micelle Substances 0.000 description 8
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 7
239000007853 buffer solution Substances 0.000 description 7
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
230000008827 biological function Effects 0.000 description 6
239000002736 nonionic surfactant Substances 0.000 description 6
229920001184 polypeptide Polymers 0.000 description 6
108090000765 processed proteins & peptides Proteins 0.000 description 6
102000004196 processed proteins & peptides Human genes 0.000 description 6
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 5
102000018697 Membrane Proteins Human genes 0.000 description 5
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 5
230000000694 effects Effects 0.000 description 5
239000000203 mixture Substances 0.000 description 5
241000196324 Embryophyta Species 0.000 description 4
125000000217 alkyl group Chemical group 0.000 description 4
125000005037 alkyl phenyl group Chemical group 0.000 description 4
ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 4
238000004925 denaturation Methods 0.000 description 4
230000036425 denaturation Effects 0.000 description 4
150000002500 ions Chemical class 0.000 description 4
239000012528 membrane Substances 0.000 description 4
ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 4
229910052757 nitrogen Inorganic materials 0.000 description 4
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
210000004027 cell Anatomy 0.000 description 3
230000000052 comparative effect Effects 0.000 description 3
238000001816 cooling Methods 0.000 description 3
239000003398 denaturant Substances 0.000 description 3
238000000502 dialysis Methods 0.000 description 3
MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 3
229940043264 dodecyl sulfate Drugs 0.000 description 3
150000002632 lipids Chemical class 0.000 description 3
239000003960 organic solvent Substances 0.000 description 3
238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 3
238000006116 polymerization reaction Methods 0.000 description 3
238000005063 solubilization Methods 0.000 description 3
230000007928 solubilization Effects 0.000 description 3
239000000243 solution Substances 0.000 description 3
239000000126 substance Substances 0.000 description 3
101710195281 Chlorophyll a-b binding protein Proteins 0.000 description 2
101710143415 Chlorophyll a-b binding protein 1, chloroplastic Proteins 0.000 description 2
101710181042 Chlorophyll a-b binding protein 1A, chloroplastic Proteins 0.000 description 2
101710091905 Chlorophyll a-b binding protein 2, chloroplastic Proteins 0.000 description 2
101710095244 Chlorophyll a-b binding protein 3, chloroplastic Proteins 0.000 description 2
101710127489 Chlorophyll a-b binding protein of LHCII type 1 Proteins 0.000 description 2
101710184917 Chlorophyll a-b binding protein of LHCII type I, chloroplastic Proteins 0.000 description 2
101710102593 Chlorophyll a-b binding protein, chloroplastic Proteins 0.000 description 2
ROSDSFDQCJNGOL-UHFFFAOYSA-N Dimethylamine Chemical compound CNC ROSDSFDQCJNGOL-UHFFFAOYSA-N 0.000 description 2
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
108010034715 Light-Harvesting Protein Complexes Proteins 0.000 description 2
KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 2
LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
241000219315 Spinacia Species 0.000 description 2
235000009337 Spinacia oleracea Nutrition 0.000 description 2
108010089860 Thylakoid Membrane Proteins Proteins 0.000 description 2
238000000862 absorption spectrum Methods 0.000 description 2
229910001870 ammonium persulfate Inorganic materials 0.000 description 2
239000003833 bile salt Substances 0.000 description 2
229940093761 bile salts Drugs 0.000 description 2
239000003093 cationic surfactant Substances 0.000 description 2
150000001768 cations Chemical class 0.000 description 2
229930002868 chlorophyll a Natural products 0.000 description 2
229930002869 chlorophyll b Natural products 0.000 description 2
NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 2
238000000432 density-gradient centrifugation Methods 0.000 description 2
238000001879 gelation Methods 0.000 description 2
238000002523 gelfiltration Methods 0.000 description 2
238000005259 measurement Methods 0.000 description 2
238000013508 migration Methods 0.000 description 2
230000005012 migration Effects 0.000 description 2
125000004433 nitrogen atom Chemical group N* 0.000 description 2
102000039446 nucleic acids Human genes 0.000 description 2
108020004707 nucleic acids Proteins 0.000 description 2
150000007523 nucleic acids Chemical class 0.000 description 2
230000029553 photosynthesis Effects 0.000 description 2
238000010672 photosynthesis Methods 0.000 description 2
239000002685 polymerization catalyst Substances 0.000 description 2
-1 polyoxyethylene dodecyl ether Polymers 0.000 description 2
238000001556 precipitation Methods 0.000 description 2
238000007873 sieving Methods 0.000 description 2
238000010186 staining Methods 0.000 description 2
210000002377 thylakoid Anatomy 0.000 description 2
238000011282 treatment Methods 0.000 description 2
DRDCQJADRSJFFD-UHFFFAOYSA-N tris-hydroxymethyl-methyl-ammonium Chemical compound OC[N+](C)(CO)CO DRDCQJADRSJFFD-UHFFFAOYSA-N 0.000 description 2
HKJKONMZMPUGHJ-UHFFFAOYSA-N 4-amino-5-hydroxy-3-[(4-nitrophenyl)diazenyl]-6-phenyldiazenylnaphthalene-2,7-disulfonic acid Chemical compound OS(=O)(=O)C1=CC2=CC(S(O)(=O)=O)=C(N=NC=3C=CC=CC=3)C(O)=C2C(N)=C1N=NC1=CC=C([N+]([O-])=O)C=C1 HKJKONMZMPUGHJ-UHFFFAOYSA-N 0.000 description 1
108091006112 ATPases Proteins 0.000 description 1
102000057290 Adenosine Triphosphatases Human genes 0.000 description 1
102100025287 Cytochrome b Human genes 0.000 description 1
108010075028 Cytochromes b Proteins 0.000 description 1
AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
241000588724 Escherichia coli Species 0.000 description 1
QUSNBJAOOMFDIB-UHFFFAOYSA-N Ethylamine Chemical compound CCN QUSNBJAOOMFDIB-UHFFFAOYSA-N 0.000 description 1
239000000232 Lipid Bilayer Substances 0.000 description 1
BAVYZALUXZFZLV-UHFFFAOYSA-O Methylammonium ion Chemical compound [NH3+]C BAVYZALUXZFZLV-UHFFFAOYSA-O 0.000 description 1
OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
108010013381 Porins Proteins 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical class OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
SLINHMUFWFWBMU-UHFFFAOYSA-N Triisopropanolamine Chemical class CC(O)CN(CC(C)O)CC(C)O SLINHMUFWFWBMU-UHFFFAOYSA-N 0.000 description 1
241001464837 Viridiplantae Species 0.000 description 1
239000002253 acid Substances 0.000 description 1
239000013543 active substance Substances 0.000 description 1
238000001042 affinity chromatography Methods 0.000 description 1
239000011543 agarose gel Substances 0.000 description 1
239000003513 alkali Substances 0.000 description 1
210000001557 animal structure Anatomy 0.000 description 1
125000000129 anionic group Chemical group 0.000 description 1
238000000429 assembly Methods 0.000 description 1
230000000712 assembly Effects 0.000 description 1
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
KQQCTWHSWXCZHB-UHFFFAOYSA-N azane;propan-2-ol Chemical compound N.CC(C)O KQQCTWHSWXCZHB-UHFFFAOYSA-N 0.000 description 1
239000003899 bactericide agent Substances 0.000 description 1
239000003012 bilayer membrane Substances 0.000 description 1
238000010504 bond cleavage reaction Methods 0.000 description 1
UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
239000006172 buffering agent Substances 0.000 description 1
239000003054 catalyst Substances 0.000 description 1
125000002091 cationic group Chemical group 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
239000001752 chlorophylls and chlorophyllins Substances 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
239000000470 constituent Substances 0.000 description 1
238000010276 construction Methods 0.000 description 1
NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
238000004132 cross linking Methods 0.000 description 1
238000002425 crystallisation Methods 0.000 description 1
210000004748 cultured cell Anatomy 0.000 description 1
CSMFSDCPJHNZRY-UHFFFAOYSA-M decyl sulfate Chemical compound CCCCCCCCCCOS([O-])(=O)=O CSMFSDCPJHNZRY-UHFFFAOYSA-M 0.000 description 1
238000007872 degassing Methods 0.000 description 1
HPNMFZURTQLUMO-UHFFFAOYSA-O diethylammonium Chemical compound CC[NH2+]CC HPNMFZURTQLUMO-UHFFFAOYSA-O 0.000 description 1
238000010494 dissociation reaction Methods 0.000 description 1
230000005593 dissociations Effects 0.000 description 1
DDXLVDQZPFLQMZ-UHFFFAOYSA-M dodecyl(trimethyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+](C)(C)C DDXLVDQZPFLQMZ-UHFFFAOYSA-M 0.000 description 1
239000003814 drug Substances 0.000 description 1
230000001516 effect on protein Effects 0.000 description 1
238000000909 electrodialysis Methods 0.000 description 1
238000005370 electroosmosis Methods 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
238000005194 fractionation Methods 0.000 description 1
102000034238 globular proteins Human genes 0.000 description 1
108091005896 globular proteins Proteins 0.000 description 1
235000011187 glycerol Nutrition 0.000 description 1
LPTIRUACFKQDHZ-UHFFFAOYSA-N hexadecyl sulfate;hydron Chemical compound CCCCCCCCCCCCCCCCOS(O)(=O)=O LPTIRUACFKQDHZ-UHFFFAOYSA-N 0.000 description 1
OHMBHFSEKCCCBW-UHFFFAOYSA-N hexane-2,5-diol Chemical compound CC(O)CCC(C)O OHMBHFSEKCCCBW-UHFFFAOYSA-N 0.000 description 1
125000001183 hydrocarbyl group Chemical group 0.000 description 1
230000001771 impaired effect Effects 0.000 description 1
239000007788 liquid Substances 0.000 description 1
YFVGRULMIQXYNE-UHFFFAOYSA-M lithium;dodecyl sulfate Chemical compound [Li+].CCCCCCCCCCCCOS([O-])(=O)=O YFVGRULMIQXYNE-UHFFFAOYSA-M 0.000 description 1
FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical group [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
238000002156 mixing Methods 0.000 description 1
239000002808 molecular sieve Substances 0.000 description 1
YNAVUWVOSKDBBP-UHFFFAOYSA-O morpholinium Chemical group [H+].C1COCCN1 YNAVUWVOSKDBBP-UHFFFAOYSA-O 0.000 description 1
229910052760 oxygen Inorganic materials 0.000 description 1
239000001301 oxygen Substances 0.000 description 1
238000005192 partition Methods 0.000 description 1
229910052698 phosphorus Inorganic materials 0.000 description 1
239000011574 phosphorus Substances 0.000 description 1
239000000049 pigment Substances 0.000 description 1
102000007739 porin activity proteins Human genes 0.000 description 1
239000011148 porous material Substances 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
230000001376 precipitating effect Effects 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
239000003755 preservative agent Substances 0.000 description 1
230000002335 preservative effect Effects 0.000 description 1
235000004252 protein component Nutrition 0.000 description 1
UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
JUJWROOIHBZHMG-UHFFFAOYSA-O pyridinium Chemical compound C1=CC=[NH+]C=C1 JUJWROOIHBZHMG-UHFFFAOYSA-O 0.000 description 1
125000001453 quaternary ammonium group Chemical group 0.000 description 1
238000010526 radical polymerization reaction Methods 0.000 description 1
238000011160 research Methods 0.000 description 1
239000002151 riboflavin Substances 0.000 description 1
235000019192 riboflavin Nutrition 0.000 description 1
229960002477 riboflavin Drugs 0.000 description 1
239000011734 sodium Substances 0.000 description 1
URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
URLJMZWTXZTZRR-UHFFFAOYSA-N sodium myristyl sulfate Chemical compound CCCCCCCCCCCCCCOS(O)(=O)=O URLJMZWTXZTZRR-UHFFFAOYSA-N 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
239000001488 sodium phosphate Substances 0.000 description 1
229960000776 sodium tetradecyl sulfate Drugs 0.000 description 1
230000003381 solubilizing effect Effects 0.000 description 1
239000002904 solvent Substances 0.000 description 1
238000002336 sorption--desorption measurement Methods 0.000 description 1
241000894007 species Species 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
239000008107 starch Substances 0.000 description 1
150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
CSMFSDCPJHNZRY-UHFFFAOYSA-N sulfuric acid monodecyl ester Natural products CCCCCCCCCCOS(O)(=O)=O CSMFSDCPJHNZRY-UHFFFAOYSA-N 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
230000009182 swimming Effects 0.000 description 1
CBXCPBUEXACCNR-UHFFFAOYSA-N tetraethylammonium Chemical compound CC[N+](CC)(CC)CC CBXCPBUEXACCNR-UHFFFAOYSA-N 0.000 description 1
QEMXHQIAXOOASZ-UHFFFAOYSA-N tetramethylammonium Chemical compound C[N+](C)(C)C QEMXHQIAXOOASZ-UHFFFAOYSA-N 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
GSEJCLTVZPLZKY-UHFFFAOYSA-O triethanolammonium Chemical compound OCC[NH+](CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-O 0.000 description 1
ZMANZCXQSJIPKH-UHFFFAOYSA-O triethylammonium ion Chemical compound CC[NH+](CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-O 0.000 description 1
GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 1
FVJLCPJDDAGIJE-UHFFFAOYSA-N tris(2-hydroxyethyl)-methylazanium Chemical compound OCC[N+](C)(CCO)CCO FVJLCPJDDAGIJE-UHFFFAOYSA-N 0.000 description 1
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1

Landscapes

Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Medicines Containing Plant Substances (AREA)
Peptides Or Proteins (AREA)

JP60163230A 1985-07-24 1985-07-24 生体膜蛋白質の分離精製法 Granted JPS6222796A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP60163230A JPS6222796A (ja)	1985-07-24	1985-07-24	生体膜蛋白質の分離精製法

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP60163230A JPS6222796A (ja)	1985-07-24	1985-07-24	生体膜蛋白質の分離精製法

Publications (2)

Publication Number	Publication Date
JPS6222796A JPS6222796A (ja)	1987-01-30
JPH0529040B2 true JPH0529040B2 (de)	1993-04-28

Family

ID=15769797

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP60163230A Granted JPS6222796A (ja)	1985-07-24	1985-07-24	生体膜蛋白質の分離精製法

Country Status (1)

Country	Link
JP (1)	JPS6222796A (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN111566210B (zh) *	2018-01-12	2025-01-07	安捷伦科技有限公司	使用三羟基和四羟基季铵化合物作为电泳分离的拆分剂

1985
- 1985-07-24 JP JP60163230A patent/JPS6222796A/ja active Granted

Also Published As

Publication number	Publication date
JPS6222796A (ja)	1987-01-30

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Rabilloud et al.	2000	Solubilization of proteins in two-dimensional electrophoresis
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