JPH09301883A - Cell activating agent containing Scutellaria barba extract and α-hydroxy acid and its application - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a safe and highly efficient cell activator that contains a blackberry lily extract and α-hydroxy acid, and a composition for percutaneous application and bathing which contains this cell activator and is useful for beauty culture, such as prevention of skin aging, skin condition improvement, wound treatment, etc. SOLUTION: This cell activator comprises an extract obtained from iridaceous plants and blackberry lily as well as α-hydroxy acid or its salt. The extract is obtained from dried blackberry lily or dried rhizomes of blackberry lily by extraction with one or more solvent selected from water, lower alcohols and organic polyol solvents. This cell activator is added to different agents for percutaneous application for daily use, and able to develop such efficacy as improvement of skin condition, wound healing, beauty culture, and keep the skin moist, lustrous, soft, pliable and supple, thereby maintaining essential homeostasis of the skin. The combination of the blackberry lily with α-hydroxy acid improves the cell activation efficacy of the composition and improves stability of the efficacy.

Description

Detailed Description of the Invention

[0001] The present invention relates to a novel plant-derived cell activator and its application. More specifically, Iridaceae plants (Belamcanda chinensi)
s L.) or an extract from a dried product of a rhizome (crude drug name: irrigation), and a useful and highly safe cell activator containing α-hydroxy acid or a salt thereof and a method for applying the same. Is.

[0002]

BACKGROUND OF THE INVENTION The cell activating agent according to the present invention has an excellent activating effect on cells and is extremely safe. Therefore, for example, when used in combination with various external preparations such as a skin external preparation, a scalp / hair preparation, and a bath preparation, cell metabolism is activated and skin aging is prevented, or skin quality is improved and wounds are improved. A cosmetic effect such as healing can be expected. Specifically, lotions, emulsions, creams, oils, packs, shampoos, rinses, hairnics, hair liquids, body soaps, or bath agents (liquid, powdery, granular, solid, etc. It can be applied to other applications. In addition to external preparations such as the above, for example, nutritional supplementation (nutritional supplement)
It can also be used by being mixed with foods and beverages for the purpose of maintaining health such as.

[0003]

2. Description of the Related Art Some plants have a therapeutic or inhibitory effect on various diseases, or have various useful physiological activities, and such useful plants have long been used for health maintenance. Has been widely used for. The iris of the family Iridaceae used in the present invention (the dried product of rhizome is called crude drug name: suiho) is said to have effects such as anti-inflammatory, antitussive / expectorant action, or antimicrobial action, especially sore throat and sore throat. , A plant that has been used as a folk medicine for the treatment of bronchial asthma.

The components contained in the Japanese cedar tree include irigenin and tectorigeni.
n), or their glycosides iridin (Iridi
n), Tectoridin, Others Belamcandin, Irisflorentin
Isoflavone compounds such as ntin) have been identified.

Further, the pharmacological action is described, for example, in [Encyclopedia of Chinese Medicine, Shogakukan (1985.12.10)] and the like. According to this, hyaluronidase (involvement in type I allergy) (Enzyme)), decoction and bathing agent of Hyougi against pathogenic dermatophytes, and alcohol extract has a hypotensive effect.

[0006] As a conventional technique relating to the application of Hyogi,
For example, JP-A-63-30417 discloses the use of five isoflavone compounds in Japanese cedar as an anti-allergic agent due to their PCA reaction inhibitory effect (anti-immediate allergic effect).
It has been proposed that the hydroalcoholic extract shown in No. 58-11922 is applied to skin whitening cosmetics by its thyrodinase activity inhibitory action.

[0007]

DISCLOSURE OF INVENTION Problems to be Solved by the Invention Based on the usefulness of plants that have been transmitted for a long time, the elucidation of biological mechanism of action such as pharmacological action and physiological activity, and the application method based on them. Although such research has been conducted, there are still few that are actively used effectively as a form reflected in general daily life.

[0008] The present inventors have searched for a plant component useful for beauty and health and examined its general use based on the theme of cell activation. In the process, an extract of Hydrangea vulgaris superior to the glycolytic enzyme pyruvate kinase, which is directly involved in the production of ATP (adenosine triphosphate) essential for cell activation. It was found that it exhibited an active action, and the application was filed first (Japanese Patent Laid-Open No. 7-83200).
-138179).

[0009] The present invention aims to further develop it, enhance the effect of the extract of Astragalus sinensis, and stabilize the formulation to further improve the utility value, and it is safe and highly effective. It provides a stable cell activating agent.

[0010]

[Means for Solving the Problems] An extract obtained by using one or more selected from water, a lower alcohol and a polyol organic solvent from a dried product of Japanese cedar or its rhizome, and an α-hydroxy acid or Include the salt.
Examples of the α-hydroxy acid used in the present invention include glycolic acid, citric acid, malic acid, tartaric acid and lactic acid.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION An extract and an α-hydroxy acid obtained by using one or more kinds selected from water, a lower alcohol and a polyol-based organic solvent from a dried product of Hydrangea vulgaris or rhizome thereof. It comprises a cell activating agent containing the salt, and a skin external preparation composition and a bath agent composition containing the same.

[0012]

[Examples] A cell is the smallest unit that constitutes a major part of each organ or tissue in an organism and is mainly responsible for those special functions. The original synthesis, decomposition,
It has all the functions of energy conservation, discharge of products, etc. and some defense mechanism, and their integrated functions. In these cells, if the division, new generation, or part of the function is reduced, the living body loses homeostasis and causes various symptoms and diseases, so research on cell aging is biological or medical. It has been promoted by a number of researchers from an academic point of view.

In particular, regarding the skin tissue, the tissue cells constituting the epidermis, dermis, and subcutaneous tissue are controlled by nutrients and regulatory factors from blood, lymph, etc. To maintain the homeostasis of the living body. In other words, the epidermis plays an important role in preventing external irritation and invasion of foreign substances, and in protecting the loss of body fluids and electrolytes from the inside of the skin (barrier function), and the dermis plays an important role as a supporting tissue of the skin. Is fulfilling.

However, when the metabolism of cells forming these tissues is deteriorated due to some factor, leading to a decrease in proliferative ability and various functions, the skin loses moisture, flexibility, and suppleness, and stains and wrinkles occur. It increases and it is easy to get hurt.
In addition, the rate of granulation and the rate of wound healing are reduced, sometimes leading to secondary diseases.

In order for cells to perform functions such as division, proliferation, differentiation or maintenance of homeostasis, at least supply of ATP which is an energy source is indispensable. The major supply of ATP in skin cells is considered to be due to glycolysis. It is known that 11 kinds of enzymes are actually involved in the reaction from glucose to pyruvic acid and lactic acid in the glycolytic system.
Pyruvate kinase (PK) is an enzyme that is directly involved in the production and is also involved in the irreversible reaction (the rate-limiting step of glycolysis) from phosphoenolpyruvate to pyruvate. Therefore, based on the idea that the activation of this enzyme is greatly involved in the activation of skin cells, the PK activation action of various plant extracts was evaluated in a test system using guinea pig skin.

As a result, PK which is excellent in the extract of Hyougi
An active action was observed, and based on this fact, the oxygen consumption test of guinea pig liver homogenate and the wound healing effect using rabbit were also examined. As a result, the efficacy was confirmed in both cases.

Further, various studies were conducted to enhance the effect and improve the stability, and earnestly researched for the purpose of making it a cell activator having high utility value.
The inventors have found that they can be achieved by further combining α-hydroxy acid with the extract of Hyougi, and completed the present invention. Examples will be shown below to describe the present invention in more detail.

[0018]

Example Pyruvate kinase (PK) activating action [Preparation of sample] a. 20% Ethanol extract of Scutellaria b. Extract of Hyogi with 20% 1,3-Butylene glycol c. Ethanol, 1,3-Butylene glycol, water (1:
1: 3) Extract with mixed solution d. Lactic acid + a Hyogi extract (dissolve lactic acid in 0.1% concentration) e. Glycolic acid + b smelt extract (dissolving glycolic acid in 0.1% concentration) f. A solution of Na citrate + c.
Dissolved in 0.1% concentration) g. A solution of sodium lactate + glycolic acid + a in a cedar extract (Na lactate: glycolic acid = 1: 1 dissolved in 0.5% concentration) h. 0.1% sodium lactate solution i. 0.1% glycolic acid solution (Note that the evaporation residue of each Hyogi extract is 0.5 to 1.5
% Is about)

[Animal used] Hartley female guinea pig, weighing about 300 g, 5 animals each

[Test Method] 1) Administration of Samples Both ventral sides of guinea pigs were shaved with hair clippers to prepare test and control groups. A lint cloth containing 1 mL of the sample was adhered to the test section with a tape and fixed with a polyethylene film for 4 hours. The control section was similarly treated with each solvent. This operation was performed once a day for two consecutive weeks. still,
The hair was shaved once every two days.

2) Preparation of skin extract 48 hours after the final administration of each extract of Hyougi, the guinea pigs were sacrificed by exsanguination, and the test and control skins were separated. The skin obtained is shaved with a hair clipper to completely remove the subcutaneous tissue, then exactly 2 g is separated and finely cut with scissors, then 20 mL under ice cooling.
The physiological saline solution was added and sonicated. This suspension
After centrifugation at 5,000 G for 20 minutes, the supernatant was used as a skin extract.

3) Measurement of enzyme activity The following substrate mixture was prepared in advance, 4 mL of it was sampled, 100 μL of guinea pig skin extract was added thereto, and the mixture was incubated at 37 ° C. for 20 minutes. Substrate mixture Tris-HCl (pH = 7.5) 100mL Phospho (enol) pyruvate 3Na 0.2g MgCl ₂ 0.02g KCl 0.02g ADP 2Na 0.02g For determination, the generated pyruvic acid was reacted with 2,4-dinitrophenylhydrazine to determine the absorbance. 510 nm was measured and it calculated | required by the following formula. The enzyme activity was converted into the amount of protein determined by the Biuret method, and the activity of the test plot when the control plot was set to 100% was estimated from the T table.

[Equation 1]

[Test Results] Of the samples used in the test, a
~ C is 113.8-117.3% (95% confidence interval) compared to the control
There is an enzyme activity action of 129.4 compared to the control group in d ~ g
A clear predominant difference was observed in the enzyme activity of ~ 136.2% (95% confidence interval). In addition, in h to i, it was 98.8 compared to the control group.
No significant effect was observed at ~ 103.1% (95% confidence interval).

[0024]

Example: Oxygen consumption capacity test [Preparation of sample] a. 20% Ethanol extract of Hyougi was concentrated to dryness under reduced pressure, and 0.0001% and 0.00001% solutions were prepared with purified water. b. Furthermore, 0.00001% of glycolic acid was added to this. c. 0.00001% solution of glycolic acid

[0025] [suspension Krebs-Ringer Bicarbonate (KBR) Adjustment] 0.90% NaCl 100 mL 1.15% KCl 4 mL 1.22% CaCl 2 3 mL 2.11% KH 2 PO 4 1 mL 3.82% MgSO 4 · 7H 2 O 1 mL 21 mL of 1.30% NaHCO ₃ Total 130 mL The pH was adjusted to 7.4 with 8.5% phosphoric acid.

[Preparation of Liver Homogenate] The liver of a Hartley guinea pig (female) was taken out, washed thoroughly with physiological saline, and homogenized in ice water by adding the same amount of KRB to prepare a 50% liver homogenate.

[Measuring equipment] TAIYO O ₂ UPTESTER (manufactured by Taiyo Kagaku Kogyo Co., Ltd.)

[Measurement of Oxygen Consumption] Sample 1.
Add 0 mL, KRB 2.0 mL, and liver homogenate 0.5 mL. Instead of the sample, add equal amount of purified water to the control. Also, set a blank without addition of liver homogenate. Attach a filter paper moistened with CO ₂ absorbent (2N-NaOH) to the lid of the container and seal it. The container is placed in a 37 ° C. thermostat and incubated for 15 minutes, then measurement is started, and the amount of water that has moved through the capillary tube is read from the scale plate every 5 minutes. The amount of air that has moved in the capillary from the blank under the influence of atmospheric pressure is calculated, and the true oxygen consumption is calculated.

[Test Results] FIG. 1 shows the change over time in oxygen consumption.
Shown in The 20% Ethanol extract of Astragalus membranaceus of the sample a had the effect of increasing the oxygen consumption in the oxygen consumption test using guinea pig hepatocytes. Further, in b, a higher value was shown. The same examination was conducted on the extracts of other solvents according to the present invention, and the effect substantially equivalent to this was obtained.

[Figure 1]

[0030]

Example: Wound healing effect [Preparation of test drug] a. 20% Ethanol extract of Hyogi b. Ethanol extract of cedar c. Extract of Hyogi with 20% 1,3-Butylene glycol d. Extract of Hyogi with 1,3-Butylene glycol e. 20% Propylene glycol extract of Hyogi f. Ethanol, 1,3-Butylene glycol, water (1:
1: 3) Extract with mixed solution g. Lactic acid + a cedar extract (dissolving lactic acid in 0.1% concentration) h. Glycolic acid + c cedar extract (dissolving glycolic acid in 0.1% concentration) i. Lactic acid: Glycolic acid = 1: 1 0.1% aqueous solution (The evaporation residue of each Hyougi extract is 0.5 to 1.5
%) Each of the above samples (2 mL) was mixed with a hydrophilic ointment (10 g) to prepare a mixture. Further, as a control, one prepared by adding only each solvent was prepared.

[Animal used] White Japanese rabbit, female, weight around 3,500 g, 3 animals

[Test Method] The inner ear of a rabbit was shaved with a depilatory cream, and 24 hours later, under anesthesia, two artificial circular ulcers having a depth of 0.2 mm were formed with a trepan, tweezers, and a scalpel each having a diameter of 5 mm. , Created at intervals of about 1 cm. After confirming hemostasis from the ulcer site, a sample ointment was applied to the inner surface of the left auricle, and a control ointment was applied to the inner surface of the right auricle. From the next day, the ulcer part was regularly copied on a transparent film and the area was measured by an image analyzer. The ointment was applied once a day after measuring the wound.

[Test Results] While the area on the 10th day after the start of the test is 14.5 to 15.6 mm ² in each control section,
In the test section, in samples a to f, 3.8 to 5.7 mm ²
The effect was recognized in. Also, in g to h, 3.1 to
A higher effect of 4.8 mm ² was obtained. Incidentally, i was 7.9 to 10.6 mm ² .

[0034]

[Examples] Safety test 1) Primary skin irritation test Ethanol, 1,3-Butylene glycol, water (1: 1: 3)
Rabbits with 3 parts of lactic acid: glycolic acid: citric acid = 1: 1: 1 mixed and dissolved in the extract of E. Weight 3,80
About 0 g) was applied to the skin. Judgment is 24,4 after pasting
At 8,72 hours, erythema and edema were used as indexes by the primary irritancy scoring method. As a result, all animals were judged as negative without any erythema or edema.

2) Cumulative skin irritation test Ethanol, 1,3-Butylene glycol, and water (1: 1: 3) of Japanese cedar
Lactate N was added to the extract (of the evaporation residue is about 1.4%)
a: Na glycolate: Na citrate = 1: 1: 1
Hair removal from the flank of a mixture of 2% and 2% (2 x 4 cm ² )
Hartley guinea pigs (female, 5 per group, weight 3)
About 20 g) was applied to the skin once a day, 5 times a week, at 0.5 mL / animal. Application was performed over 4 weeks and hair removal was performed on the last application day of each week. The judgment was made on the day following the last day of each week by using primary irritation scoring method with erythema and edema as indices. As a result, in all animals, 1-
No erythema or edema was observed over the 4th week, and the result was negative.

3) Acute toxicity test The solvent was completely distilled off under reduced pressure from the Ethanol extract of Hyogi, and Na lactate: Na glycolate: N citrate.
2,000 mg / kg to ddy mice (1 group, 5 mice, body weight 30 g), which were prepared by mixing equal amounts of a = 1: 1: 1 and fasting for 4 hours before the test.
The amount was orally administered, and the onset and degree of toxicity were observed over time. As a result, no abnormalities were observed in all the mice for 14 days, and no abnormalities were observed in the dissection results. LD ₅₀
Was determined to be 2,000 mg / kg or more.

[0037]

[Examples] Application to various preparations When the cell activating agent according to the present invention is applied to produce various preparations, pharmaceuticals, quasi-drugs, cosmetics, bath agents, foods and beverages as shown below are used. Among various components and additives usually used in the above, those suitable for the purpose can be arbitrarily selected and used in combination.

(1) Various oils and fats Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange lafa oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukui nut oil, safflower oil, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil , Castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, oak, coconut oil, beef tallow, lard, milk fat, or hydrogenated products of these fats (hardened oil, etc.) Such.

(2) Waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, etc.

(3) Mineral oil Liquid paraffin, petrolatum, paraffin, ozokeride, ceresin, microcristan wax, squalene, squalane, pristane, etc.

(4) Fatty acids Lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, natural fatty acids such as lanolin fatty acid, isononanoic acid, caproic acid , 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2
-Synthetic fatty acids such as ethylhexanoic acid and isopentanoic acid.

(5) Alcohols ethanol, isopropyl alcohol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, and phytosterol; and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol, etc.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, etc.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate, etc.

(9) Gum, sugar and water-soluble polymer compound gum arabic, benzoin gum, dammar gum, guaiac butter, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose and its esters, trehalose and its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or hydroxyalkyl (C ₂ -C ₄ ) oxide to which alkylene (C ₂ -C ₄ ) oxide such as ethylene oxide is added _{2 to} C ₄ ) chitin or chitosan, low molecular weight chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate and its salt, heparin, ethyl cellulose , Methylcellulose, carboxymethylcellulose, carboxyethylcellulose, sodium carboxyethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, nitrocellulose, crystalline cellulose Polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylic acid salts, polyalkylene oxide or crosslinked polymer thereof such as polyethylene oxide or polypropylene oxide, carboxyvinyl polymers, such as polyethyleneimine.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate ester salt, alkyl phosphate ester salt), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate type amphoteric surfactant, nonionic surfactant (ether type nonionic) Surfactants, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants), other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactant, surfactant containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A ₁ ), dehydroretinal (vitamin A ₂ ), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (Vitamin B ₁ ), riboflavin (vitamin B ₂ ), pyridoxine (vitamin B ₆ ), cyanocobalamin (vitamin B ₁₂ ), folic acid,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: ascorbic acid and its derivatives, vitamin D group: ergocalciferol (vitamin D ₂ ), cholecalciferol (vitamin D ₃ ),
Dihydrotaxosterol, vitamin E group: tocopherol and its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K ₁ ), menaquinone (vitamin K ₂ ), menadione (vitamin K ₃ ), menadiol (vitamin K ₄ ), and the like.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, etc. and their sulfates, phosphates, nitrates, citrates, or amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are processings (for example, crushing, milling, washing, hydrolysis, etc.) which are conventionally carried out depending on the product type and form to be added. Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, powderization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. are arbitrarily selected and combined, and various materials are selected. You can select and use it. The solvent used for extraction may be selected in consideration of the purpose and type of the product to be provided, the processing to be performed later, and the like. Usually, water or a water-soluble organic solvent (eg ethanol, propylene glycol) is selected. , 1,3-butylene glycol, etc.)
It is desirable to use one or a mixture of two or more selected from the above. However, when the content of the organic solvent is not preferable depending on the use, only water may be used, or ethanol which is easily removed after extraction may be used, and may be used alone or in an arbitrary mixture with water.

Additives derived from plant or animal raw materials are
When applied to systemic or topical external preparations and cosmetics, including protection of skin and hair, moisturizing, improvement of feel and texture,
Gives flexibility, relieves irritation, relieves stress due to aroma, activates cells (prevents cell aging), suppresses inflammation, improves skin and hair quality, prevents and improves rough skin, hair growth, hair growth, hair loss prevention, gloss Application, cleaning effect, alleviation of fatigue, promotion of blood flow,
In addition to cosmetic effects such as a warm bath effect, effects such as flavoring, deodorizing, thickening, preserving, and buffering can be expected. Furthermore, in addition to these, various cosmetic and pharmaceutical effects of each known raw material are expected, and by combining these, the intended effect can be enhanced or multifunctional. It is also possible to make a product that expects an effect.

As a concrete plant (crude drug) used as a raw material,
For example, Ai (Aiba), Aokazura (Seifu Fuji), Aotsu-Juraji (Kibo), Acacia, Akasugi fruit, Akagrape, Akamegashiwa (Akabu Kashiwa), Akane (Akane grass root), Akayajiou (Jihuang), Agi (Awei), Akinire (Roasted skin), akebi
(Kidori), Asa (Jin Asako), Asagao (Toshiko), Ashitaba
(Tomorrow's leaf), Adzuki (red azuki bean), Asenyaku (Asenyaku), Acerola, Anise fruit, Abemaki fruit, Avocado, Ama, Amacha (Amacha), Amadokoro (Tamatake), Amigasa lily (Shellfish), Almond, Altea, Arnica , Aloe (log), aloe vera, apricot (almond jelly), ansokkou (benzuka), Igakozolina (biliary head), knotweed (tiger cane root), strawberry, fig (non-flower fruit) or its leaves, strawberry (winter aoiko),
Itohimehagi (Enshi), carob, rice seed or seed coat,
Inondo seeds, nettle, ylang-ylang, ginkgo (gingko leaves, ginkgo), Ibuki-yakosou, fennel (fenka), ukiyagara (sanryou), turmeric fruit, turmeric
(Utsubane), Usbania Oyster, Usbenita Oyster, Usubasaishin (spicy), Utsubogusa (summer hay), Udo or Shishiudo (Qang activity, independence, Tang Dynasty activity), Ume (Umebai) or its pulp, Vladigashi, Uwaurushi, Unshurikan ( Chen), Estragon, Eleuthero (Ezo Goka), Enoki, Ebisugusa (Keiko), Elderberry fruit, Elemi, Enju
(Gossip, Gossip rice), ogre (Astragalus radiata), Ouren (Orchid), Ookarasuri (Karonin), Ootsu-la-Fuji (self-defense), Psyllium (Carmae, Carmaegrass), Toucan (Haruko), Obananaokera ( White ajutsu), fruit beetle fruit, hawthorn
(Yamako), barley (barley), ogurma (turning over), okra fruit, okera (white jujube), panax ginseng (carrot), hypericum (brother's cut grass), odorikosou (intermittent), onamimi (blue ears),
Origurumi, Oninoyagara (Tianma), Oniyuri (Yuri), Oninos, Halibut (Hihayu), Ominaeshi (defeated), Dutch strawberry, Dutch garashi, Dutch parsley, Dutch honeyspot, olive, oregano, orange fruit or peel,
Kaikei (ripe yellow), seaweed [green algae, brown algae, red algae],
Cacao seeds, oysters or their leaves (persimmon), kagi kazura (chou hooks), cassia, casino ki fruit (pepper fruit), gajutsu (shadoju),
Kashiwa (Shaju, Shayo), Cascarilla, Kanixa (Kin Sato),
Valerian (valerian root), squash, capokunoki seed, kahokusansho (pepper), cattail (kamaki), chamomile, kamiyatsude (passing grass), karasuri (flower), oak grass, rasabisaku (midsummer), guarana seeds, karaya, Karin (Gourd Gourd), Kawayanagi, Kawatake Mushroom, Kawamuragi (Inchinko), Kansui (Kansu), Licorice (Licorice), Candelilla, Kanto,
Canna, raspberry, kiwi fruit, kikarasuri (uryu root), kikyo (bellflower, bellflower root), chrysanthemum (chrysanthemum flower), catalpa
(Azumi), Rumex (Yotei root), Kizuta, Kidachi aloe, Kidachi mint, Kinoki, Yellowfin (yellow), Yellowtail
(Astragalus), Gymnema Sylvesta, Kiraja Saponaria, Kiraya, Cabbage, Cabbage Immature Fruit, Cucumber, Kumquat Fruit, Betel, Kinmizuki (Senakurushi), Guayule, Quercus Infectoria (Gallery), Kukuinuts, Kuko (Garium, Gugiko, gugi leaf, ground bone bark), cedar japonicus (tenmon winter), kuzu (kuzukon), camphor tree, gooseberry fruit, gardenia (garden garden), kunugi (bokuso), kumazasa,
Japanese black bean (Coleoptera), Clara (Bittersweet), Cranberry fruit, Chestnut or its astringent skin, Curculigo latifolia fruit, Grapefruit fruit, Buckthorn buckthorn, Black grape mochi (Needs rescue), Kurobana cypress (Enmeisou), Chlorella, Mulberry (Mulberry leaf, Mulberry fruit, mulberry fruit, mussels (thorns, stalks), celosia (cow flower, rooster), keirin saishin (spicy), laurel, gentian, gennoshoko
(Old tomato grass), Cilantro fruit, Koshinbara (Monthly flower), Kosui mint, Kozo fruit, Black tea, Kouhone (Raw bone), Kouhon (Straw and Kara straw), Koulyan, Kokemomo (Koshibachi),
Koganebana (Yellow Gon), Kogomeba Hypericum, Coconut Fruit, Goshuyu (Goshuyu), Goshostrawberry (covered bonko), Pepper (pepper), Copaiba Balsam, Coffee Beans, Kobushi
Burdock, burdock, burdock, sesame
(Sesame seeds), sesame seeds (brown ginseng), wheat (wheat), rice or rice bran (red bran, white bran), cola acuminata seeds, cola vera seeds, fenugreek fruit, colombo, condullango, kelp, comfrey ), Konjac, Sakisima button d'zuru (weird sen), cherry blossoms, leaves, fruits, bark (cherry bark),
Pomegranate, sasa, lily of the valley, lily of the valley, sasanqua, sardines of the sardines (sawashatsu), sweet potato, sugarcane, sugar beet,
Jujube jujube (acid jujube), saffron (ban safflower, western safflower),
Pomelo fruit, Soapwort, Salmonberry fruit, Sarashina-sho (Masahima), Salvia, Sawagikyo (Yamakona),
Sawagurumi leaves, Sankirai (Yamakoi), Hawthorn (Yamako), Sanshin ginseng (Sanjin ginseng), Sanshuyu (sanshuyu), Sansho (sansho), Sandscon (Yamazone), Cyanoki fruit, Shiitake mushroom, Jio (Jiro), Shion (Shi-en), Digitalis, Shikunshi (Kimiko), Shishiudo (Independent, Tang-do),
Perilla, Aojiso, Chirimenzo, Catamendiso (Shiyo leaf, Shiso), Weeping birch (white birch bark), Rosewood, Linden tree, Chinese beetle (Forestry), Shimakaniku (Chrysanthemum flower), Shimotsukesou, Potato, Peony (Shakuyaku), Shajin
(Sasan), Scotch beetle (Wheat gate winter), Shirakashi seed, Shirogoshi butterfly seed, Shirobanairisu, Shirobanatsuta flower, Shirominanten (South Tenmi), Juzudama, Palm fruit, Ginger (Ginger), Shoubu (Iris, Iris root) , Shozuku fruit, Birch, Cinconas sacyl bra, Cinnamon, Watermelon
(Watermelon), honeysuckle (gold and silver flowers, shintofu), sorrel (acidic pattern), swimberry fruit, strawberry fruit, horsetail (infestation), stevia, plum fruit, pine cone, pine larch, euphorbia edulis, edible walnut, Pear fruit, snowy pear, oilseed meadow, oilseed americanum, oilseed walnut, oilseed smelt, mint, euphoria melanogaster, euphoria dolphini, edible mackerel, euphorbia, saccharomyces, sacrum Kawakyu), Senna fruit or leaves, Senninsou (large tate), Japanese cabbage (medicine), Soka (grass), buckwheat seeds, Somei potato, Daio (Ohio), Radish, soybean, Daidai (orange peel, Kikomi), Thyme , Takatodai (Daigei), Takawarabi (Inu), Dark Sweet Ferry fruit, Thymus vulgaris (HyakuRika), Tachibana (Tachibanakawa), Tachiyanagi, tamarind seeds, onions, Magnolia salicifolia (fist), Aralia elata or the root bark, Danshen (Dan Shen), dammar, cherry fruit,
Chigaya or its roots (Chiyone), chicory, cloves (cloves, cloves), daffodil (gomiko), daffodil
(Ohta), Korean ginseng (Wuyi), Korean ginseng
(Ginseng), Artemisia (Aiba), Choreimaitake
(Irei), Tsukimiso, Camellia, Camellia, Tsumekusa (lacquer lacustrine), Tsurukusa (duckling grass), Tsuruazuki (red azuki bean), Tsurudukudami (Hanshukara), Tsuruna (Kankyo), Tsurujinjin (Four leaf ginseng), Tsuwabuki, Deiko , Teuchigurumi, Duberry fruit, Tengusa, Tendaiyaku (crow medicine), Touga (Winter melon), Pepper (Pan pepper), Touki (Toki), Toukinsenka, Maize or Maize hair (Nanban hair), Tosumi Mochi (Female Sadako) , Tourindo (dragon gall), dokudami (jujutsu), radish (tomato), tochiban ginseng (bamboo ginseng), eucommia (tochu, tochuha), tomato, tragacanth, triacanthos seed, tormentilla, dronoki, troloi, nigeriaberry fruit , Potatoes (herbal medicine), nagi-kida, naginatakoju, nazuna, jujube, jujube (daijutsu),
Naniwaibara (Kanekurako), Narco Lily (Yoshisei), Peanut (Peanut), Nanten (Namitenmi), Nigaki (Makiki), Nigayomogi (Gakui), Nikuzuku, Nikkei (Kisushi), Leek (Nirako),
Elderberry (bone grafted), carrot, garlic (garlic), nurde
(Five-fold child), leek, thistle (daiji), noibara (fruit), yarrow, nodake (maehu), nobara, peach, palm,
Hibiscus (Bussouge, Furin-Bussouge, Roselle), Pineapple fruit, Hakata lily (Yuri), Hakaumauraboshi (Bone crushing supplement), Hakusen (White moss bark), Huckleberry fruit, Hakobe (Resurrection), Hazelnut (Haruko), Hashidokoro (Roth root) , Basil, lotus (lotus meat, lotus root), parsley, batata, wasp (bamboo-boiled), patchouli, mint (light load, light load leaf), barley (yokuinin), hanasuge (chimomo), banana, hanahakka, vanilla bins, papaya, Stilt
(Mouse malt grass), hub grass, paprika, hamago (vine thorns), hamasuge (kosuke), hamabishi (situriko), hamanas (my rose flower), hamabafu (beach storm), hamamelis, rose (rose), parietalia , Harunile (Yu, YuYi, Yuba),
Breadfruit, lizard carrot (participant), pecan nuts, hemlock (stone garlic, vine pearl scent), Hikiokoshi (prolonged life grass), Hishi (Hishimi), pistachio, beet, Hittsuba (stone reed), Hinako no Kozuchi (cow knee) , Hiba, castor, sunflower, bell pepper, himemagama (scented pepper), Himematsutake, Pimenta fruit, Amaranthus fruit, Birodoaoi, Broadbrush
(Hakuhaku), Hiroha Senega (Senega), loquat (Silver, loquat leaf), Betel nut (large abdominal skin, Renko), Futokazura (Nanto), butterbur (Saika Fuyu), Fukitampo (Sakai Fuyu, Sakai Fuyu),
Fujibacama (Orchid grass), Fuji bean (flat bean), butcher bloom, grape skin or fruit, beech, Fuyusuminashikutaketake
(Caterpillar), Brazilian licorice, black currant fruit, blackberry fruit, plum fruit, fluceraria,
Blueberry fruit, prune, blond psyllium, bundou (mung bean), loofah, safflower (safflower), belladonna,
Berry fruit, Boysenberry fruit, Boufu (Windbreak), Spinach, Physalis (Torone), Honoki (Park, Japanese Koboku), Bokeh (wood melon), Hosobaokera (Soburaku), Hosobabarengiku, Bodaiju, button (peony skin), Hop, jojoba,
White berry fruit, hon apricot (apricot kernel), honshimeji, maizerutennansho (tennan star), maoh (mao),
Macadamia nuts, Macuri (Marine grass), Magwa (Mulberry leaves, Mulberry bark), Bamboo shoots (Boiled bamboo), Matatabi (Ten Taki tree), Matsukasa,
Matsuhodo, Marigold, Malvano jadine
(Apricot ginseng), Mulberry fruit, Quince, Marronnier, Mango, Mangosteen, Manchurian walnut, Mandarin fruit, Ganoderma lucidum (Ganoderma lucidum), Mannenrow, Citrus fruit (Crop fruit), Mishima Psycho (Saiko), Mizokukushi (Halfen lotus) ， Misohagi (Senkyaku), honeywort, honeywort, honeywort (vine vine), green mint, mimosa, myoga,
Miracle Fruit Fruit, Myrrh, Myrobalan, Starling
(Kokaki), Mukunoki, Makuji, Purple (Murasaki), Purple corn, Purple corn, Swordfish (Ichimokuso), Mebuki, Melaleuca, Melissa, Melilot, Melon fruit, Mukoyomogi, Mosouchi, Magnolia (spicy), Mockweed (woody incense),
Maple butter, fruit of peach, seed (peach kernel) or leaf (peach leaf), bean sprout, morelo cherry fruit, morohaya, yakawamurasakiki, yakchi (maschi), cornflower, cornflower, cornflower, yashabushi (arrow wheel), yachiyanagi ， Matsude (octagonal metal plate), Mistletoe (willow parasite), Willow tree leaf, porcupine, Yabukouji (purple gold bull), pokeweed
(Commercial land), Yamano yam (mountain drug), Yamanoki (Yamaharu), bayberry (Yangmei skin), eucalyptus, Yucca brebifolia, Yukinoshita (tiger ear grass), yuzu fruit, lily, mugwort (spoiler leaf), yellow grass, lime Fruits, peanuts, fruits of rachis, fruits of raspberries, lavender, longan (dragon eye meat), green tea, apple fruits, rubus suavisims (letter gourd), lettuce, red currant fruits, lemon fruits, lemongrass, forsythia, lotus root, There are wax palm, loganberry fruit, rose hip, rosemary, Roman chamomile, seaweed, wasabi, waremoko (earthen worm).

Materials derived from animal-derived raw materials include poultry extract, cow / human placenta extract, pig / cow stomach or duodenum or intestinal extract or its decomposition product, pig / cow spleen extract. Or its decomposition products, extracts of brain tissue of pigs and cows,
Water-soluble collagen, collagen derivative such as acylated collagen, collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, keratin and its degradation product or their derivatives, silk protein and its degradation product or their derivatives, Pig / Bovine blood cell proteolysis product (globin peptide), Pig / cow hemoglobin degradation product (hemin, hematin, heme, protoheme, heme iron, etc.), milk,
Casein and its degradation products or their derivatives, skim milk powder and its degradation products or their derivatives, lactoferrin or its degradation products, chicken egg components, fish meat degradation products, etc.

(14) Microbial culture metabolites Yeast metabolites, yeast extract, rice fermentation extract, rice bran fermentation extract, Euglena extract, lactic acid fermented product of skim milk powder, etc.

(15) Inorganic pigment Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(16) Ultraviolet absorber p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B ₆ derivatives, oxybenzone, benzophenone, guaiazulene, shikonin, baicalin, baicalein, berberine and the like.

(17) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid (including catechin compound) Such.

(18) Antioxidant Ascorbic acid and its salt, stearic acid ester, tocopherol and its ester derivative, nordihydroguaselethenoic acid, butylhydroxytoluene (BHT),
Butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol and the like.

(19) Anti-inflammatory agent Ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-
Camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts and the like.

(20) Disinfectant / Disinfectant Acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or its hydrolyzate.

(21) Fragrances Fragrances of natural animals such as musk, civet, catrium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayak Wood Essential Oil, Cumin Essential Oil, Black Letter Essential Oil, Cayenne Essential Oil, Cinnamon Essential Oil, Geranium Essential Oil, Copaiba Balsam Essential Oil, Coriandel Essential Oil, Perilla Essential Oil, Cedarwood Essential Oil, Citronella Essential Oil, Jasmine Essential Oil, Gingergrass Essential Oil, Cedar Essential Oil, Spearmint Essential Oil, Western Mint essential oil, large scented essential oil, tuberose essential oil, Choji essential oil, orange essential oil, winter green essential oil, true balsam essential oil, butchery essential oil, rose essential oil, palmarosa essential oil, cypress essential oil, hiba essential oil,
Sandalwood essential oil, Petitgren essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(22) Dye / coloring agent Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, ende dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, whole buckwheat pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

(23) Other moisturizers, hormones, sequestering agents, pH adjusters,
Chelating agents, antiseptic / antifungal agents, cooling agents, stabilizers, emulsifiers, animal / vegetable protein and its degradation products, animal / vegetable polysaccharides and its degradation products, animal / vegetable glycoprotein and its degradation products, Blood flow promoters, anti-inflammatory agents / anti-allergic agents, cell activating agents, keratolytic agents, wound healing agents, foaming agents, thickening agents, oral agents, deodorizing / deodorizing agents, enzymes, etc. When used in combination, additive and synergistic effects can be expected.

[0064]

EXAMPLES Preparation of Various Formulations Several topical formulations were prepared using the cell stimulant derived from Hyougi according to the present invention. The formulation examples are shown below, but the use of the cell activating agent according to the present invention is not limited to these.

1) Production Example of Lotion A lotion was produced according to the following formulation. % By weight Sorbit 2 2.1,3-butylene glycol 2 3. Polyethylene glycol 1000 1 4. Polyoxyethylene oleyl ether (25E.O.) 25 Ethanol 10 6. Hyougi extract (20% Ethanol ex.) 10 7. Sodium lactate 0.2 8. pH adjuster qs 9. Preservative appropriate amount 10. Purified water 100 residue

2) Production Example of Emulsion An emulsion was produced according to the following formulation. % By weight Squalane 3 2. Vaseline 1 3. Stearyl alcohol 0.3 4. Sorbitan monostearate 1.55. Polyoxyethylene (20) sorbitan monooleate 3 6.1,3-butylene glycol 5 7. Hyougi extract (20% 1,3-Butylene glycol ex.) 58. Glycolic acid 0.19. Preservative appropriate amount 10. Purified water 100 residue

3) Cream Production Example A cream was produced according to the following formulation. % By weight Squalane 20 2. Beeswax 5 3. Refined jojoba oil 5 4. Glycerin monostearate 2 5. Sorbitan monostearate 2 6 Polyoxyethylene (20) sorbitan monostearate 2 7. Glycerin 5 8. Hyougi extract (1,3-Butylene glycol ex.) 5 9. Sodium citrate 0.2 10. Preservative appropriate amount 11. Purified water 100 residue

4) Shampoo Production Example A shampoo was produced according to the following formulation. % By weight Triethanolamine lauryl sulfate 5 2. Polyoxyethylene lauryl ether sulfate Na 12 3.1,3-butylene glycol 4 4. Lauric acid diethanolamide 2 5. Disodium edetate 0.1 6. Hyougi extract (20% 1,3-Butylene glycol ex.) 10 7. Sodium tartrate 0.2 8. Preservative appropriate amount 9. Purified water 100 residue

5) Production Example of Hair Liquid A hair liquid was produced according to the following formulation. % By weight Ethanol 29 2. Polyoxypropylene butyl ether phosphoric acid 10 3. Polyoxypropylene monobutyl ether 5 4. Triethanolamine 1 5. White cedar extract (Ethanol, 1,3-BG, water (1: 1: 3) ex.) 5 6. Glycolic acid 1 7. Preservative appropriate amount 8. Purified water 100 residue

6) Production Example of Body Soap A body soap was produced according to the following formulation. % By weight Potassium laurate 15 2. Potassium myristate 5 3. Propylene glycol 5 4. Hyougi extract (20% Propylene Glycol ex.) 15 5. Sodium citrate 0.1 6. Sodium lactate 0.1 7. pH adjuster Appropriate amount 8. Preservative appropriate amount 9. Purified water 100 residue

7) Production Example of Bath Agent (A Type) A bath agent was produced according to the following formulation. % By weight Sodium hydrogen carbonate 55 2. 2. anhydrous sodium sulfate 30 Borax 2 4. 4. Dry powder of Hyogi extract (40% Ethanol ex.) 10 5. Sodium lactate 26. Sodium glycolate 1

8) Production Example of Bath Agent (B Type) A bath agent was produced according to the following formulation. % By weight Refined jojoba oil 5 2. 2. Polyoxyethylene sorbitan monolaurate 20 Glycerin monostearate 5 4. Liquid paraffin 2 5. Lauric acid diethanolamide 3 6. Hyougi extract (50% Propylene glycol ex.) 25 7. Malic acid 18. Sodium lactate 1 9. Purified water 100 residue

[0073]

[Example] Use test of various external preparations Male and female panelists (15 persons each) were allowed to freely use the lotion and the bath preparation manufactured in the example for one month, and a questionnaire survey on feeling of use was requested. The results are shown in Table 1.
It is as follows.

[Table 1]

[0074]

The cell activating agent according to the present invention is excellent in its effect and highly safe. By blending this with various skin external preparations that are routinely used and using them repeatedly, cosmetic effects such as an effect of activating skin cells to improve skin quality and a wound healing effect can be obtained. That is, it is possible to maintain the original homeostasis of the skin by keeping the skin moisturized, glossy, flexible, and supple. The combined use of α-hydroxy acid with the extract of Hyougi enhances the cell activation effect and also improves the stability. The cosmetic effect of the cell activating agent according to the present invention and the application method based on the cosmetic effect are useful for human beauty and health by an extremely daily method, and the effective utilization of plants is expanded. The effect on top is great.

[Brief description of drawings]

FIG. 1 is a graph showing the effect of the cell activating agent according to the present invention on the oxygen consumption of guinea pig hepatocytes. FIG.
FIG. 4 is a graph showing the effect of 20% Ethanol extract of Hyougi on oxygen consumption in guinea pig hepatocytes. FIG.
FIG. 4 is a graph showing the effect of glycolic acid on oxygen consumption of guinea pig hepatocytes.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Office reference number FI Technical display area A61K 7/48 A61K 7/48 7/50 7/50 31/19 ADA 31/19 ADA // ( A61K 35/78 31:19)

Claims (3)

[Claims] 1. An extract obtained by using one or more selected from water, a lower alcohol and a polyol organic solvent from a dried product of the iris family Hyogi or rhizome thereof, and an α-hydroxy acid or the same. Cell activating agent containing salt 2. A skin external preparation composition containing the cell activating agent according to claim 1, which comprises an extract from a dried product of the iris family Hyogi or rhizome thereof, and an α-hydroxy acid or a salt thereof. 3. A bath agent composition containing the cell activator according to claim 1, which contains an extract from a dried product of the iris family Hyogi or rhizome thereof and an α-hydroxy acid or a salt thereof.