JPH0987189A - Antiallergic agent containing isodon japonicus hara, paeonia suffruticosa andrews, perilla frutescens britton var. acuta kudo, and/or arunica montana linne - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new and safe antiallergic agent, antihistaminic agent, anticomplement activator, hyaluronidase activity inhibitor, arachidonic acid metabolic activity inhibitor, contact dermatitis inhibitor, and further, a skin preparation for external use and bathing agent. SOLUTION: This agent is obtained as an extract from one or more plants selected from among Isodon japonicus Hara, Paeonia suffruticosa Andrews, Perilla frutescens Britton var, acuta Kudo and Arunica montana Linne with water, ethanol, 1,3-butylene glycol, propylene glycol or a mixture of two or more of these extractants, and used as an antiallergic agent, antihistaminic agent, anticomplement activator, hyaluronidase activity inhibitor, arachidonic acid metabolic activity inhibitor and contact dermatitis inhibitor, and furthermore used as a skin preparation for external use and bathing agent, with efficacy in preventing and healing different types of dermatitis, including allergic, atopic and contact dermatitis, or eczema, skin chapping, skin itching, dry skin, etc., and also has high safety.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an anti-allergic agent, anti-histamine agent, anti-complement activator, hyaluronidase activity inhibitor, arachidonic acid containing one or more plant extracts selected from nematodes, button pipi, perilla, and arnica. Metabolic activity inhibitor, contact dermatitis inhibitor, effective for prevention and improvement of allergic / atopic / contact skin inflammation or skin diseases such as eczema, itch, rough skin, and dryness of the skin, and safety The present invention relates to a highly effective external preparation for skin or bath agent.

[0002] The field of application is generally applicable to various internal and external preparations (including preparations used for animals), and specifically, ampules, capsules, pills, tablets, and powders. 1) Pharmaceuticals, 2) Quasi-drugs, 3) Foods, 4) Topical or systemic skin cosmetics, 5) Scalp / hair Pharmaceutical and / or cosmetic preparations (eg, shampoos, rinses, treatments, permanent solutions, hair dyes,
Hair styling agents, hair styling agents, hair restoration and hair nourishing agents, etc.) 6) Bath agents to be used by throwing them in a bath.

As foods, oral compositions (gum, candy, etc.), processed fish paste products such as kamaboko and chikuwa, livestock products such as sausage and ham, Western confectionery, Japanese confectionery, raw noodles, Chinese noodles, boiled foods. Noodles such as noodles and buckwheat, sauces, soy sauce, sauce, sugar, honey, seasonings such as powdered candy and starch syrup, curry powder, mustard powder, pepper powder and other spices, jam, marmalade, chocolate spread, pickles, soy vegetables , Sprinkles, processed vegetables and fruits such as canned and bottled vegetables and fruits, dairy products such as cheese, butter, yogurt, fruit juice, vegetable juice, whey beverages, soft drinks, beverages such as alcoholic beverages, etc.
Use in general foods and drinks such as health foods is raised.

[0004]

2. Description of the Related Art It is said that the number of people having skin problems such as skin irritation, urticaria and atopy continues to increase year by year, and it is said that one in five people, including infant eczema, is present. Among them, dermatitis caused by allergies, including those that develop in other organs such as hay fever and asthma, is particularly regarded as a problem, and is now said to be a national disease.

[0005] Essentially, living organisms have an immune function that works to protect themselves by eliminating foreign substances such as pathogenic microorganisms. However, this function sometimes works to injure the body and may cause various diseases. The impaired reaction caused by the immune function is particularly called an allergic reaction, and is classified into two groups, an immediate allergy and a delayed allergy, according to the time from the reaction to the onset of the allergy. It is classified into four types of IV.

[0006] Type I is the type most frequently occurring as an allergy, and is a reaction mainly involving immunoglobulin E (IgE) antibodies. This IgE is produced against allergens that have entered the body and has a strong effector effect on mast cells and basophils. Mast cells and basophils (a type of leukocyte) Cells having granules containing pharmacologically active amines such as serotonin, the former being present around blood vessels and connective tissues, and the latter being present in blood. On these cell membranes, a receptor that binds an IgE antibody is present and has a property of strongly binding to the IgE antibody, and for an IgE antibody bound to mast cells or basophils,
When the same type of allergen binds again, a number of active substances such as histamine and serotonin described above are released from these cells with degranulation, causing various allergic symptoms,
Symptoms such as itchy redness or swelling rash (urticaria) on the skin, inflammation of the nose and eyes and itching and increased secretion of nasal secretions and tears, or difficulty in breathing due to clogged trachea Symptoms such as bronchial asthma are classified as allergic diseases of this type.

[0007] In the type I allergy, the process can be roughly divided into three stages, which are classified into each stage based on their action points, and prevent or prevent allergic diseases by administering a drug or the like.
Attempts to alleviate or treat have been made. That is,
When foreign antigens enter the body, IgE antibodies are produced by immunocompetent cell lines, and the IgE antibodies are distributed in airway, skin, digestive organs and other mast cells,
Alternatively, sensitization is established by sticking to basophils in the blood. This is the reaction referred to as the first step.

Next, when the sensitized cells are again contacted with an antigen, the cells undergo morphological changes such as vacuolation, swelling, and degranulation, and release chemical transmitters such as histamine, serotonin, and SRS-A. I do. This is the second stage reaction.

The released chemical messenger acts on contraction of smooth muscles such as bronchial muscle and gastrointestinal tract, enhancement of capillary permeability, neutrophil migration, platelet aggregation, etc., resulting in asthma, lower back pain and the like. The process of developing allergic symptoms such as gastrointestinal allergy with diarrhea, nasal allergy and urticaria is the third stage reaction.

Therefore, drugs acting in the first and second stages in a narrow sense are prophylactic drugs for allergic reactions and
Those acting at the stage can be considered as symptomatic treatments. Currently, the most active research on antiallergic drugs is the development of drugs that suppress this second stage.

[0011] Type II allergy is known as a damaging reaction caused by the destruction of tissue cells by IgG and IgM antibodies. IgG and IgM antibodies that act on this system significantly activate the complement system when linked to an antigen. (Effector action). The reaction mechanism is based on the fact that IgG and IgM antibodies bind to antigen cells such as microorganisms, thereby activating the complement system and destroying target cells.

[0012] The function of the complement system is to activate the protease system in a chain on target cells to form and destroy the membrane damage complex (MAC), and to generate a group of fragments called anaphylatoxins. It takes a complex process such as promoting histamine release from mast cells and attracting polymorphonuclear leukocytes and macrophages to promote foreign body treatment. However, such reactions are caused by virus-infected autologous cells, tissue cells or erythrocytes to which haptens (substances that are not antigenic in nature but produce antibodies when bound to proteins, etc.) or bacteria are bound or adhered. In an immune disease, even if the cells are in their own normal cells, the living body recognizes and causes them as antigen cells. Typical examples of type II allergic diseases include aplastic anemia and hemolytic anemia.

[0013] Type III allergy is a reaction in which a large amount of an antigen-antibody conjugate formed by binding of an antibody to an antigen is deposited on a tissue and damaged. Antibodies involved in this reaction are mainly IgG antibodies, which are reactions involving many factors such as the complement system and polymorphonuclear leukocytes, similar to type II, but the difference from type II is that the antigen is not a cell but a substance. Is a major feature. still,
The reaction mechanism is that the produced antigen-antibody conjugate is deposited on tissues such as blood vessels, kidneys, joints and skin to activate the complement system (effector effect). As a result, a large amount of anaphylatoxin is produced, which increases vascular permeability, contracts smooth muscle, and promotes the release of histamine from mast cells, resulting in inflammation.

In addition, the action of phagocytic cells such as polymorphonuclear leukocytes accumulated by the action of anaphylatoxin causes a lysosomal degranulation reaction of the cells, and various types of degradative enzymes such as proteases in the lysosome are released to the outside. This leads to further tissue and cell damage and an allergic inflammatory response. The factors that cause damage to tissues and cells by the action of phagocytic cells are not only the effects of the released enzymes, but also the excess active oxygen produced by promoting respiration with phagocytosis. The action of is also considered important, excess active oxygen,
It has been pointed out that it has undesirable effects on living organisms, such as damage to normal tissue cells, pigmentation, and aging of cells.

Next, in the case of type I-III allergies, the reaction takes a relatively short time after contact with the antigen (a reaction appears in about 2 to 3 minutes, and the intensity of the reaction becomes maximum in about 10 minutes). In contrast to a type IV reaction, which is called an immediate allergy because of the appearance of an impaired reaction, the reaction begins to appear only a few hours after the reaction with the antigen, and the reaction progresses slowly thereafter. It is called delayed allergy because it takes about 24-48 hours to reach the highest strength.

A characteristic of type IV allergy is that no antibodies such as those found in the immediate form are involved, but instead lymphocytes called T cells are involved. That is, when T cells sensitized by an antigen are activated again by the antigen, various inflammatory factors called lymphokines (macrophage chemotactic factor, lymphocyte chemotactic factor, macrophage activating factor, vascular permeability factor) , Etc.), which are thought to cause a reaction to be amplified. Among the skin reactions in delayed allergy, contact dermatitis characterized by redness, swelling, induration, and infiltration of mononuclear cells into the local area is well known.

Various test methods have been proposed based on the allergic reaction types classified in this way, and approaches to factors effective for preventing or ameliorating allergic diseases have been actively conducted.

[0018]

For example, for type I allergy, antispasmodics that relax smooth muscle, sympathomimetics that suppress the increase in capillary permeability, and antihistamines are also mentioned. However, these are all drugs that act in the third stage and are effective as symptomatic treatments, but most of them are synthetic drugs and have problems in terms of side effects. Furthermore, active research is being conducted on the development of agents effective against type II-III and type IV allergies, but the current situation is that no specific antiallergic agent has been found yet.

[0019]

In view of the above circumstances, the present inventors have made various plants or ingredients useful as antiallergic agents a theme of development, and as a result, have extracted plants of Phellinus linteus, Botanpi, Perilla, Arnica. The substance has an antihistamine action, an anti-complement activity action, a hyaluronidase activity inhibition action, an arachidonic acid metabolic activity inhibition action, and a contact dermatitis inhibitory action, so that an effective factor for suppressing immediate to delayed allergies ( The present invention provides a highly safe external preparation for external use and bath preparation, which is effective in preventing and improving skin inflammation (for example, redness, eczema, edema, swelling), itch, rough skin, and dryness of the skin. Therefore, the present invention has been completed.

[0020]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, the term "Emnesia japonicum" refers to a plant of the Labiatae family
sodon japonicus Hara), or S. japonicus Hara)
on trichocarpus Kubo) Above ground or whole grass. or,
"Button pie" is a button of the family Paeoniaceae.
(Paeonia suffruticosa Andrews) root bark, "Perilla" means perilla frutescens Br.
Itton var.acuta Kudo) or its related plant (Labiatae) leaves and branch tips, and "Arnica" means the Asteraceae (Composita
e) Plants Flowers or roots of Arnica montana Linne are used.

[0023] The extracts of Tricholoma communis, Pleurotus cornucopiae, Perilla frutescens, and Arnica used in the present invention mean the parts of each plant body (above ground part, whole grass, root bark, leaves, branch tips, flowers, roots) as they are or after dried. And extracted with a solvent. As the extraction solvent, any one selected from water, ethanol, 1,3-butylene glycol and propylene glycol,
Alternatively, two or more kinds can be used in an arbitrary combination, and can also be used in a state in which each of water, ethanol, 1,3-butylene glycol and propylene glycol extraction is combined. Further, the obtained extract is applied as an anti-allergic agent, anti-histamine agent, anti-complement activator, hyaluronidase activity inhibitor, arachidonic acid metabolism inhibitor, contact dermatitis inhibitor, and further skin external preparation and bath preparation dosage form. It may be adjusted by optionally drying, concentrating, or diluting depending on the form.

The production method is not particularly limited, but it is usually within the range of the boiling point of the solvent at room temperature to atmospheric pressure, and after extraction, adsorption, decolorization It may be purified into a solution, paste, gel, or powder. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected.
An activated carbon column or the like may be used as a purification treatment means for decolorization or the like, and any ordinary means generally applied to an extracted substance may be selected.

[0023] The green leaves, pearl oysters, perilla, of the present invention,
The extract of Arnica can be used as it is as an anti-allergic agent, anti-histamine agent, anti-complement activator, hyaluronidase activity inhibitor, arachidonic acid metabolic activity inhibitor, contact dermatitis inhibitor, and can be blended into external skin agents and bath agents. However, the amount to be blended is not particularly specified,
Although it may vary slightly depending on the type and quality of various action inhibitors or cosmetics and the expected degree of action, it is usually 0.05% by weight or more (hereinafter referred to as% by weight), preferably 1 to 40%. If the blending amount is less than 0.05%, the effect cannot be expected sufficiently.

The anti-allergic agent, anti-histamine agent, anti-complement activator, hyaluronidase activity inhibitor, arachidonic acid metabolic activity inhibitor, contact dermatitis inhibitor, skin external preparation and bath preparation of the present invention are the above-mentioned essential components. In addition to the
The components and additives used in the preparation of pharmaceuticals, quasi drugs, cosmetics and the like can be used in combination within a range that does not impair the effects of the present invention.

For example, oils and fats (avocado oil, almond oil, fennel oil, perilla oil, olive oil, orange oil,
Orange rafer oil, sesame oil, cacao butter, chamomile oil,
Carrot oil, cucumber oil, beef tallow, tallow fatty acid, kukui nut oil, safflower oil, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, Cocoa butter, palm oil, palm kernel oil, mokuro, coconut oil, beef tallow,
(Pig fat, hydrogenated oil, hydrogenated castor oil, etc.)

Waxes (beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, etc.)

Mineral oil (liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcristan wax, polyethylene powder, squalene, squalane,
(Pristan etc.)

Fatty acids (lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12
-Hydroxystearic acid, undecylenic acid, tall oil, natural fatty acids such as lanolin fatty acid, isononanoic acid,
Caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-
Synthetic fatty acids such as methylpentanoic acid, 2-ethylhexanoic acid, and isopentanoic acid)

Alcohols (ethanol, isopropyl alcohol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol,
Cholesterol, natural alcohol such as phytosterol, synthetic alcohol such as 2-hexyldecanol, isostearyl alcohol, 2-octyldodecanol),
Furthermore, polyhydric alcohols (ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide,
Propylene glycol, polypropylene glycol, 1,
3-butylene glycol, glycerin, batyl alcohol, pentaerythritol, sorbitol, mannitol, glucose, sucrose, etc.)

Esters (isopropyl myristate,
Isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, diethyl phthalate, dibutyl phthalate, acetic acid Lanolin, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, etc.)

Metal soap (aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate, etc.)

Gum and water-soluble polymer compounds (arabic gum, benzoin gum, dammal gum, guaiac fat, Irish moss, karaya gum, tragacanth gum, carob gum, quince seed, agar, casein, dextrin,
Gelatin, pectin, starch, carrageenan, carboxyalkyl chitin or chitosan, hydroxyalkyl chitin or chitosan, low molecular weight chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid and its salt, hyaluronic acid and its salt,
Chondroitin sulfate and its salts, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, poly Acrylate, polyalkylene oxide such as polyethylene oxide or polypropylene oxide or cross-linked polymer, carboxyvinyl polymer, polyethyleneimine, etc.)

Surfactant "Anionic surfactant (carboxylate, sulfonate, sulfate ester salt, phosphate ester salt)", "cationic surfactant (amine salt, quaternary ammonium salt)", "amphoteric interface" Activator: Carboxylic acid type amphoteric surfactant (amino type, betaine type), Sulfate ester type amphoteric surfactant, Sulfonic acid type amphoteric surfactant, Phosphate ester type amphoteric surfactant "," Nonionic surfactant (Ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant,
Block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants "," Other surfactants (natural surfactants, protein hydrolyzate derivatives, polymeric surfactants, including titanium / silicon Surfactants, fluorocarbon surfactants) "

Vitamin "Vitamin A group: retinol,
Retinal (vitamin A ₁ ), dehydroretinal (vitamin A ₂ ), carotene, lycopene (provitamin A) ”,“ Vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B ₁ ), riboflavin (vitamin B ₂ ),
Pyridoxine (vitamin B ₆ ), cyanocobalamin (vitamin B ₁₂ ), folic acid, nicotinic acid, pantothenic acid, biotin, choline, inositol ”,“ vitamin C group: ascorbic acid and its derivatives ”,“ vitamin D group: ergo ” Calciferol (vitamin D ₂ ), cholecalciferol (vitamin D ₃ ), dihydrotaxosterol ”,“ Vitamin E group: tocopherol and its derivatives, ubiquinones ”,“ Vitamin K group: phytonadione (vitamin K ₁ ), menaquinone ( Vitamin K ₂ ), menadione (vitamin K ₃ ), menadiol (vitamin K ₄ ) "and others, ferulic acid, γ-oryzanol, etc.

Amino acids (valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, Arginine, ornithine, histidine, etc. and their sulfates, phosphates, nitrates, citrates, or amino acid derivatives such as pyrrolidone carboxylic acid)

Furthermore, 1 of animal tissues or plants, extracts of crude drugs (in the case of plants, water, organic solvents (ethanol, propylene glycol, 1,3-butylene glycol, etc.))
An extract extracted with one kind or a mixed solution of two or more kinds is desirable.
In addition, the animal tissue may be an extract obtained by hydrolyzing the tissue in addition to the extract obtained by the same method as the plant. These are used as moisturizing ingredients or for the purpose of various known cosmetic and medical effects. ), For example, Acenyak (Asenyaku), Ashitaba, Acerola, Altea, Avocado, Amacha (sweet tea), Aloe, Aloe vera, Nettle, Ginkgo (Ginkgo leaf, Ginkgo), Fennel (Smell incense), Turmeric (Ugane), Usubasaicin (Usubasaishin) (Spicy), Japanese apricot (Ume), Vladimir oak, Uwaurushi, Neubara (Yuomi), Ougi (Yellow 耆), Scutellaria baicalensis (Ogon), Yamazakura (Sakura), Yellowfin (Yellow), Oren (Huangren), Panax carrot , Hypericum (younger brother cut grass),
Odori Koso, Dutch pepper, orange, Itohimehagi (distinct), Nepenthes (Summer dead grass), Tsurudokudami (Several crow), Enju (Sophora), Mugwort (Guy leaf), Gajutsu (Slull), Kudzu (Kudzu root), Kanosou (Yoshikusa root) , Chamomile, chrysanthemum melon (urine root), kawamura mugwort (chinese linseed), licorice (licorice), coltsfoot (season winter flower, winter winter leaf), raspberry, kiwi fruit, kyokyo (Japanese bellflower), chrysanthemum (chrysanthemum), cowpea (Azusa) Fruit), Citrus fruit (Fruitaceae), Tachibana (Tachibana peel),
Cucumber, udo or shishido (independence, self-reliance), apricot (almond seed), wolfberry (terrestrial skin, husks, husks), clara (bitterness),
Camphor tree, Kumazasa, grapefruit fruit, Nikkei
(Keikin), Keigai (Keiguy), Ebisugusa (Keimeiko), Malva Morning Glory or Morning Glory (Ken Beef) ,, safflower (Safflower), Gobaishi (quintuplet), Comfrey, Copaiba, Gardenia (Yamasuko), Gentiana, Japanese honoki (Koho) ), Hinako no Kochi (beef knee), Goshuyu (Kurei), Burdock, Korean ginseng (Gomiza), Rice, Rice bran (Aka bran, Shiran bran), Wheat, Mishima Psycho (Saiko), Sakura, Saffron, Saponsou, Hawthorn (Mountain) Zako), Sansho (Sansho), Salvia,
Panax notoginseng (37 ginseng), shiitake mushroom (shiitake mushroom), dio (ground yellow), shikunshi (messenger), purple (purple root), perilla
(Shiso), Oyster (persimmon), Peony (peony), Psyllium
(Car maeko, car mae grass), ginger (ginger), ginger (iris),
Black-headed squirrel (Sadako), spirea, birch, honeysuckle (gold and silver flower, Shinobi winter), stevia, Ivy,
Achillea millefolium, Sambucus nigra, Azuki bean (red adzuki bean), Elderberry (bone grafting), mallow, senkyu (river kyu), senburi (our medicine), mulberry (mulberry bark, mulberry leaves), jujube (large jujube), soybean, tanaki ， Chixet carrot (Takebushi ginseng), hanasuge (mother), hibiscus (bushouge, furuinbussouge, roselle), waremoko (earthensis), dokudami (ten drug), fuyusuminakustaketake (winter caterpillar), capsicum, physalis root (Toro) ), Tachijakouso, Ryokucha (green tea), Kocha (black tea), Clove (chocolate), Unshiu mandarin orange (Chen skin), camellia, Centella asiatica, Capsicum (Pancho), Toki (Doki), Daffodil, Daidai (orange peel) , Waremoko (earthenworm), corn (nanban fur), Eucommia ulmoides (Tonchu, Morinaka leaf), tomato, nanten (southern heaven), garlic (large sun), barley (malt), Chinese cabbage (white moss bark) Ophiopogon (Ophiopogon), parsley, Batata, mint (peppermint), Hamamelis, roses, loquat leaf (loquat leaves), Wolfiporia Extensa (茯 Ryo), grapes or its leaves, loofah, linden, button
(Peony skin), hop, Maikai (My agate flower), pine needle, horse chestnut, mannen wax, sucrose, melissa, meliloth,
Bokeh (wood melon), bean sprouts, peaches (peach kernels, peach leaves), Japanese cedar (irrigation), betel nuts (cow roe), swordfish (beneficial grass), cornflower, yukinoshita (tiger ears grass), bayberry (pick ume bark),
Yashabushi (arrow wheel), pearl barley (pearl barley, Yokuinin), sagebrush, sardine, sagebrush, lavender, apple fruit, ganoderma lucidum, lemon fruit,
Forsythia (Forsythia), Forsythia vulgaris, Gentian ginger (Long-rooted grass), Hashiridokoro (roth root), Chicken gooseberry, placenta extract of cattle and humans, stomach and duodenum of pigs and cattle, or intestinal extract or its decomposition products, water soluble Collagen, water-soluble collagen derivative, collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, silk protein, silk protein hydrolyzate, bovine blood cell protein hydrolyzate, etc.)

Microorganism culture metabolites (yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, rice bran fermentation extract, Euglena extract, lactic acid fermentation product of skim milk powder, etc. ) And α-hydroxy acids (glycolic acid, citric acid, malic acid, tartaric acid, lactic acid, etc.)

Inorganic pigments (anhydrous silicic acid, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate,
(Magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, sunflower, chromium oxide, chromium hydroxide, carbon black, calamine, etc.)

Ultraviolet absorber (p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative, camphor derivative, furan derivative , Pyrone derivative, nucleic acid derivative, allantoin derivative, nicotinic acid derivative, vitamin B ₆ derivative, oxybenzone, benzophenone, guaiazulene, shikonin, baicalin, baicalein, berberine, etc.)

Astringents (lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid, etc. )

Antioxidants (ascorbic acid and its salts, stearic acid ester, tocopherol and its ester derivatives, nordihydroguaselethenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BH)
A), para-hydroxyanisole, propyl gallate,
(Sesameol, Sesamorin, Gossypol, etc.)

Anti-inflammatory agents (ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-camphor, hydrocortisone,
(Guaiazulene, chamazulen, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts, etc.)

Sterilizers / disinfectants (acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercury chrome, lactoferrin or hydrolysates thereof)

Hair agents (selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine,
Tiantor, Castali tincture, Ginger tincture,
Capsicum tincture, quinine hydrochloride, strong ammonia water, potassium bromate, sodium bromate, thioglycolic acid, etc.)

Fragrances (natural animal flavors such as musk, civet, catrium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil,
Fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guaiac wood essential oil, cumin essential oil, black character essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriander essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil , Essential oil of jasmine, essential oil of gingergrass, essential oil of cedar, essential oil of spearmint, essential oil of Western peppermint, essential oil of tubercula, essential oil of tuberose, essential oil of essential character, essential oil of orange flower, essential oil of winter green, essential oil of true balsam, essential oil of rose, essential oil of palmarosa, hinoki cypress Essential oils, Hiba essential oils, sandalwood essential oils, petitgrain essential oils, bay essential oils, vetiver essential oils, bergamot essential oils, Peruvian balsam essential oils, boad rose essential oils, mellow essential oils, mandarin essential oils, eucalyptus essential oils, lime essential oils, lavender essential oils, linaloe essential oils, lemongrass Essential oil, lemon essential oil, rosemary essential oil, vegetable flavoring such as Japanese mint essential oils, and other synthetic fragrances)

Colorants / colorants (red cabbage color, red rice color, madder color, anato color, squid color, turmeric color, ende color, krill color, persimmon color, caramel, gold, silver, gardenia color, corn color, onion Pigment, tamarind pigment, spirulina pigment, whole buckwheat pigment,
(Cherry pigment, seaweed pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.)

Sweeteners (sugar, sweet tea, fructose, arabinose, galactose, xylose, stevia, mannose, maltose, honey, glucose, miraculin, monerin, etc.)

Nutrient enhancer (calcined shell calcium, cyanocorabamine, yeast, wheat germ, egg yolk powder, hemicellulose, heme iron, etc.)

Dairy products (milk, cheese, fresh cream, butter, margarine, milk powder, whey, condensed milk, etc.)

In addition, moisturizers, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic / antifungal agents, cooling agents, stabilizers, emulsifiers, animal / vegetable proteins and their decomposed products, animal / animal Plant polysaccharides and their degradation products, animal and plant glycoproteins and their degradation products, blood flow promoters, anti-inflammatory / anti-allergic agents, cell activating agents, keratolytic agents, wound healing agents, foam thickeners, thickeners Agents, oral agents, deodorants / deodorants, bitters, seasonings, enzymes, etc., and antiallergic agents, antihistamines, arachidonic acid metabolic activity inhibitors, anticomplement activators, hyaluronidase activity inhibitors of the present invention , A contact dermatitis inhibitor and components known to date to have antiallergic action, antihistamine action, arachidonic acid metabolism inhibitory action, anti-complement activation action, hyaluronidase activity inhibitory action, contact dermatitis inhibitory action Some use can be expected additive and synergistic various effects of.

Further, the antiallergic agent, antihistamine agent, anticomplement activator, hyaluronidase activity inhibitor, arachidonic acid metabolic activity inhibitor, contact dermatitis inhibitor, as well as skin external preparations and bath preparations of the present invention may have any dosage form. And in ampoule shape,
Capsule, powder, granule, pill, tablet, solid,
Liquid, gel, foam, emulsion, cream, ointment,
It can be used by being mixed with pharmaceutical products such as sheets, quasi-drugs, cosmetics for skin and hair, and bath agents.

Specifically, for example, basic or external medicinal preparations, lotions, emulsions, creams, ointments, lotions, oils, basic cosmetics such as packs, facial cleansers, skin cleansers, shampoos, rinses, hair treatments. Hair cosmetics such as, hair cream, pomade, hair spray, hair styling agent, perm agent, hair tonic, hair dye, hair restoration and hair nourishing agent,
Makeup cosmetics such as foundation, white powder, whitening, lipstick, blusher, eyeshadow, eyeliner, mascara, eyebrows, eyelashes, etc.
Includes perfumes, bath agents, other toothpastes, mouth fresheners / gargles, liquid odor / deodorant inhibitors, sanitary products, sanitary cotton, wet tissues, etc., and also used in general foods and drinks It is possible.

The antiallergic agent, antihistamine agent, anticomplement activator, hyaluronidase activity inhibitor, arachidonic acid metabolic activity inhibitor, contact dermatitis inhibitor, and method of addition to the skin external preparation and bath agent of the present invention. Regarding, may be added in advance or may be added during the production, and may be appropriately selected in consideration of workability.

[0054]

EXAMPLES The present invention will now be described with reference to production examples, test examples, and prescription examples, but the present invention is not limited to these examples.

(Production Example 1) 100 g of Trifolium japonicum (Hiki-Okoshi or Kurobana Hiki-Okoshi) in 30% ethanol solution or
Immerse in 50% 1,3-butylene glycol solution, or 60% propylene glycol solution, or 1 kg of purified water (about 80 ° C),
After extracting at room temperature for 3 days and night, it is filtered to obtain about 0.8 kg of an extract (containing a dry solid content of 1.0 to 2.0 ^W / _V %).

(Preparation Example 2) 100 g of pea pie was added to 50% ethanol solution or 40% 1,3-butylene glycol solution or 40%
Propylene glycol solution or purified water (about 30 ℃) 1kg
And extract at room temperature for 3 days and night, and then filter to obtain about 1.0 kg of an extract (containing 0.5 to 1.5 ^W / _V % of dry solid).

(Production Example 3) 100 g of perilla was immersed in 40% ethanol solution or 30% 1,3-butylene glycol solution or 50% propylene glycol solution or 1 kg of purified water (about 40 ° C.), and the mixture was kept at room temperature for 3 days. After extracting day and night, it is filtered to obtain about 0.8 kg of extract (containing 0.5-1.0 ^w / _v % dry solids).

(Production Example 4) 100 g of Arnica was added to 40% ethanol solution or 30% 1,3-butylene glycol solution, or 50%
Propylene glycol solution or purified water (about 90 ℃) 1kg
And extract at room temperature for 3 days and night, and then filter to obtain about 0.8 kg of extract (containing dry solid content of 1.5 to 2.5 ^w / _v %).

(Test 1) Histamine Release Inhibition Test As a characteristic reaction in IgE antibody-related allergy, the release of chemical mediators (histamine etc.) from mast cells is carried out, resulting in allergic symptoms. Therefore, a substance that suppresses histamine release can be expected to have an effect of preventing and improving allergic inflammatory diseases. In this test, in the extracts obtained in Production Examples 1-4, Co, which is a histamine-releasing agent from rat mast cells, was extracted.
Test method to release histamine with mpound 48/80 (J.So
c. Cosmet. Japan, 25 (4), P246 (1992)). (Test method) a. Samples The various plant extracts were subjected to solvent distillation under reduced pressure, redissolved in purified water to a solid content concentration of 0.1 ^W / _V %, and subjected to the test. In addition, 0.1 ^W / _V % glycyrrhizin dipotassium aqueous solution was used as a positive control. b. Measurement of free histamine amount To 1.2 ml of mast cell suspension collected from the abdominal cavity of Wistar rats, 0.2 ml of sample and Compound 48/80 (final concentration 1 μg
/ Ml) was added and reacted at 37 ° C. for 15 minutes. After stopping the reaction by cooling with ice, the reaction solution was centrifuged, and the amount of released histamine was measured by the method of Shore et al. (J. Pharmacol. Exp. Therap., P12).
7,182 (1959)), and the histamine release inhibition rate was calculated by the following formula (Equation 1).

A: Fluorescence intensity of histamine released when a histamine releasing agent was added to mast cells in the presence of a test drug B: Fluorescence intensity of histamine released when a histamine releasing agent was added to mast cells C: Obesity Fluorescence intensity of histamine that is spontaneously released from cells (Note that A, B and C are the measured values minus the blinded values.)

[0061]

[Equation 1]

(Test Results) As shown in Table 1, it was confirmed that the Phellinus linteus, Phellinus linteus, Perilla frutescens and Arnica extract of the present invention had a stronger histamine release inhibitory action than the positive control aqueous solution of glycyrrhizin dipotassium.

[0063]

[Table 1]

(Test 2) Anti-Complement Activity Test The complement system is a reaction system that plays an important role in immediate allergy involving antibodies other than IgE antibodies. Substances that affect this complement system include allergies,
It may affect the formation of disease such as inflammation. In this test, the extract obtained in Production Examples 1-4 was examined using an anti-complement activity measurement method using the hemolytic reaction of sensitized red blood cells as an index. (Test method) a. Gelatin-Veronal buffer (GVB ²⁺ ) 1.7g sodium chloride, barbital 0.115g, sodium barbital 0.075g, calcium chloride 0.015g, magnesium chloride 0.010g, gelatin 0.2g, purified water 100ml are mixed and adjusted to pH 7.5. Then, the total volume was adjusted to 200 ml with purified water. b. Sheep red blood cell (SRBC) suspension The sheep blood was centrifuged at 2,000 rpm for 5 minutes, washed 3 times with physiological saline, and GVB ²⁺ was added to the precipitate to make a 10% SRBC suspension, and finally SRBC. Suspension 0.25ml to 3.05ml 0.1%
540 nm when completely hemolyzed by adding sodium carbonate solution
The absorbance was adjusted to 0.455. c. 0.2 ml of 10% SRBC suspension in anti-SRBC mouse serum was intravenously injected into the tail of an IVCS male mouse, and 4 days after that, blood was collected and the serum was separated and diluted 40-fold with GVB ²⁺ and used. d. Complement Guinea pig fresh serum was diluted 20-fold with GVB ²⁺ and used. e. After the solvent of the sample extract was distilled off under reduced pressure, the sample extract was redissolved in purified water to a solid content concentration of 0.5 ^W / _V %, and then subjected to the test.
As a positive control, 0.5 ^W / _V % aqueous solution of dipotassium glycyrrhizin was used. f. Measurement of anti-complement activity 0.1 ml sample in GVB ²⁺ 1.3 ml, 0.5 ml anti-SRBC serum, SRBC suspension
0.25 ml and a complement solution of 0.25 ml were sequentially added, and the reaction was carried out for 60 minutes in a constant temperature bath at 37 ° C. After leaving it in ice water for 10 minutes to stop the reaction, the reaction solution was centrifuged at 2000 rpm for 10 minutes to separate unlysed red blood cells, and the supernatant was 540 nm.
OD value was measured. It should be noted that, in which purified water was added instead of the sample as a control, a blank containing no serum was set for each sample and the control, and the complement activity suppression rate (= anti-complement activity effect) was calculated by the following formula (Equation 2). I asked.

[0065]

[Equation 2]

(Test Results) As shown in Table 2, it was confirmed that the Phellinus linteus, Pea pipi, Perilla frutescens and Arnica extract of the present invention had a stronger anti-complement activity than the positive control aqueous solution of glycyrrhizin dipotassium.

[0067]

[Table 2]

(Test 3) Hyaluronidase activity inhibition test Hyaluronidase is a hydrolase of hyaluronic acid distributed in connective tissue and is activated during inflammation, destroys connective tissue matrix, and penetrates cells and blood vessels of inflammatory system. It is believed to play a role in enhancing sex.
It is also known as a inflammatory enzyme, and has been used as a inflammatory agent to induce acute edema experimentally. Furthermore, it has been reported that the activity is inhibited by anti-inflammatory and anti-allergic agents (Inflammation, Vol. 4, No. 4, P. 437 (1984)). It is possible to evaluate antiallergic effects. In this test, the inhibitory effect of the extract obtained in Production Examples 1-4 was examined with reference to the Morgan-Elson method. (Test method) a. Sample Extraction solution was evaporated under reduced pressure to remove the solvent, and then purified water
It was redissolved so as to have a concentration of 0.5 ^W / _V % and used for the test. still,
A 0.5 ^W / _V % aqueous solution of glycyrrhizin dipotassium was used as a positive control. b. Measurement of hyaluronidase activity Hyaluronidase solution (final concentration 0.4 mg /
0.05 ml), leave it at 37 ℃ for 20 minutes, and then add Compound48 /
80 solution (final concentration 0.1 mg / ml) was added, and the mixture was allowed to stand at 37 ° C for 20 minutes, and then hyaluronic acid solution (final concentration 0.4 mg / m
l) 0.25 ml was added and left at 37 ° C. for 40 minutes. After stopping the reaction by adding 0.1 ml of 0.4N sodium hydroxide solution, 0.8M
0.1 ml of potassium borate solution was added and heated in boiling water for 3 minutes. After cooling to room temperature, add 3% of 1% p-dimethylaminobenzaldehyde acetic acid solution, leave at 37 ° C for 20 minutes, then
The absorbance at 85 nm was measured. In addition, the one in which purified water was added instead of the sample was used as a control, a blank containing no enzyme was set for each sample and the control, and the hyaluronidase activity inhibition rate was calculated by the following formula.

[0069]

(Equation 3)

(Test Results) As shown in Table 3, it was confirmed that the Phellinus linteus, Botanpi, Perilla frutescens, and Arnica extract of the present invention had a hyaluronidase activity inhibitory action almost equivalent to that of the positive control aqueous solution of glycyrrhizin dipotassium.

[0071]

[Table 3]

(Test 4) Arachidonic Acid Ear Edema Inhibition Test Also, in allergies involving IgE antibody, phospholipids in the cell membrane are destroyed to release arachidonic acid, which is one of the chemical mediators under the action of various enzymes. It is metabolized to prostaglandin, SRS-A, which results in various allergic symptoms. Therefore, the substance having the action of suppressing the metabolic activity of arachidonic acid can be expected to be used as an antiallergic agent. In this test, a hydrophilic vaseline ointment containing the extract obtained in Production Example 1-4 was prescribed,
The effect of 3,4-Dihydroxychalcone on mouse arachidonic acid ear edema by the method of Shinna et al .: The 113th Annual Meeting of the Pharmaceutical Society of Japan was investigated. (Test method) a. Sample The extract was concentrated by distilling off the solvent under reduced pressure, and a hydrophilic petrolatum ointment containing 10% by weight in terms of solid content was prepared and used by a conventional method. b. Measurement of edema swelling rate About 3% of the above ointment was applied beforehand with arachidonic acid.
Two and one hour before, a total of three times, a mouse (Slc: ICR female mouse, about 6 weeks old) was applied to the right auricle with thorough rubbing. Immediately before applying arachidonic acid, the ointment adhering to the auricle was wiped off, 20 μl of 5 ^w / _w % arachidonic acid (SIGMA) dissolved in acetone was applied, and the auricle was punched out (5.0 mm) after 1 hour. . Similarly, the left auricle was also excised, and the swelling rate of arachidonic acid ear edema was measured from the weight difference between the left and right auricles.
The determination was made by comparing the ear edema swelling rate of the control group to which only the base was applied as a blank, and the ear edema inhibition rate was calculated. In addition, 8-9 mice were used for the test.

(Test Results) As shown in Table 4, it was confirmed that the Phellinus linteus, Phellinus linteus, Perilla frutescens and Arnica extracts of the present invention have an activity of suppressing arachidonic acid metabolic activity.

(Test 5) Contact dermatitis inhibition test In the contact dermatitis reaction, T sensitized by the antigen was used.
Upon contact with the same antigen again, lymphocytes release various lymphokines that activate macrophages and lymphocytes, causing an inflammatory reaction. Therefore, a substance that suppresses a series of inflammatory reactions against the same antigen can be expected to be used as a delayed allergic agent. In this test, a hydrophilic petrolatum ointment containing the extract obtained in Production Example 1-4 was prescribed and the method of Nakamura et al.
980)) and its effect on the para-phenylenediamine-induced contact dermatitis reaction was examined.

(Test method) a. Sample The extract was concentrated by distilling off the solvent under reduced pressure, and a hydrophilic petrolatum ointment containing 10% by weight in terms of solid content was prepared and used by a conventional method. b. Measurement of edema swelling rate First, 0.1 ml of 2.5% paraphenylenediamine / acetone: olive oil = 4: 1 (hereinafter, PPD) was continuously applied to the shaved abdominal skin of mice (BALB / c female mice: about 8 weeks) for 3 days. After application, sensitization was carried out, and after 5 days, 20 mg of the test drug was applied to the auricle three times at intervals of 1 hour. One hour after the final application, the test drug was thoroughly wiped off, and 20 μl of PPD was applied to the auricle to induce it. 17 hours after elicitation, the test drug was again applied to the auricles that had been elicited twice at 1-hour intervals. Next, 19 hours after the stimulus, the test drug was thoroughly wiped off, and 24 hours after the elicitation, both auricles were punched to a certain area (diameter 5.0 mm) and its weight was measured.
In the determination, the swelling rate was measured from the difference in the weight of the untreated one-sided auricle, and the inhibition rate of dermatitis was determined by comparing with the control group, and a contact dermatitis inhibition test was performed. In addition, 8-9 mice were used for the test.

(Test Results) As shown in Table 4, it was confirmed that the Phellinus linteus, Phellinus linteus, Perilla frutescens and Arnica extracts of the present invention have a contact dermatitis inhibitory action.

[0077]

[Table 4]

(Test 6) Safety test (1) Primary skin irritation test The extracts of the nematodes, peony, perilla, and arnica obtained in Production Examples 1 to 4 had a dry solid concentration of about 1.0 ^W /
_It was prepared with purified water to _V % and applied to the skin of Japanese white rabbits (female, 3 animals per group, body weight around 2.3 kg) whose backs were shaved. The judgment was made 24, 48, and 72 hours after application by the primary irritation scoring method using erythema and edema as indices. The result was negative in all animals, with no evidence of erythema or edema.

(Test 7) Safety test (2) Cumulative skin irritation test Similarly, the dry extract, pea pie, perilla, and arnica extracts obtained in Production Examples 1 to 4 had a dry solid concentration of about 1.
Hartley guinea pigs (female, 3 per group, weight 32) prepared with purified water to 0 ^W / _V % and shaved flank
0.5 g / mouse was applied to the skin (about 0 g) once a day, 5 times a week. Application was performed over two weeks and shaving was performed on the last application day of each week. Judgment was made on the day of each application and on the day after the last application using the primary irritancy scoring method using erythema and edema as indices. As a result, all animals were judged as negative without any erythema and edema over 2 weeks.

(Test 8) Safety Test (3) Acute Toxicity Test Similarly, powders (dry solids) obtained by concentrating and vacuum-drying the extracts of Phellinus linteus, Peanut, Perilla, and Arnica obtained in Production Examples 1 to 4 in the same manner. before testing the minute about 1.0 ^W / _V%), 4 hours fasted ddy mice (male and female, 1 group 5 mice,
Oral administration was carried out at a dose of 2,000 mg / kg at 5 weeks of age, and the occurrence and degree of toxicity symptoms were observed over time. As a result, no abnormalities were observed in all the mice for 14 days, and no abnormalities were observed in the dissection results. Therefore, the LD ₅₀ was determined to be 2,000 mg / kg or more.

(Formulation Example) Manufacture of Various Topical Formulations According to the above-mentioned evaluation results, the formulation examples are shown below. Each formulation example may be one produced by a conventional method in the production of each product, and only the compounding amount is shown. It was Further, the present invention is not limited to these.

(Formulation Example 1) Emulsion weight% 1. Squalane 5.0 2. Olive oil 5.0 3. Jojoba oil 5.0 4. Cetyl alcohol 1.5 5. Glycerin monostearate 2.0 6. Polyoxyethylene (20) cetyl ether 3.0 7. Poly Oxyethylene (20) Soorbitan monooleate 2.0 8.1,3-Butylene glycol 1.0 9. Glycerin 2.0 10. A: Crassulaceae 30% ethanol extract (solid content 1.0%) B: Button pie 50% 1,3-butylene glycol extraction Liquid (solid content 1.0%) C: Perilla 50% ethanol extract (solid content 0.8%) D: Arnica 50% propylene glycol extract (solid content 1.8%) * Any one of A-D extract 5.0 11 .Perfume, antiseptic proper amount 12. Purified water 100 residue

(Formulation Example 2) Peel-off pack weight% 1. Glycerin 5.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 8.0 5. Polyoxyethylene glycol 1.0 6. Emmezo 50% 1,3-ethylene glycol extract (Solid content: 2.0%) 5.0 7. Perfume, preservative appropriate amount 8. Purified water 100 Residue

(Formulation Example 3) Cold cream weight% 1. White beeswax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Almond oil 15.0 5. Borax 0.5 6. Botanpi 50% ethanol extract (solid content 1.5%) 10.0 7. Perfume, antiseptic proper amount 8. Purified water 100 residue

(Formulation Example 4) Shampoo wt% 1. Triethanolamine lauryl sulfate 5.0 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3.1,3-butylene glycol 4.0 4. Diethanolamide lauric acid 2.0 5. Disodium edetate 0.1 6. Perilla 50% ethanol extract (solid content 1.0%) 10.0 7. Perfume, preservative appropriate amount 8. Purified water 100 residue

(Formulation Example 5) Body soap% by weight 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. Crassulaceae hot water extract (solid content 1.0%) 5.0 5. Arnica 50% Proroylene glycol Extraction liquid (solid content 1.7%) 5.0 6. pH adjuster proper amount 7. Preservative proper amount 8. Purified water 100 residue

(Formulation Example 6) Rinse weight% 1. Stearyl trimethyl ammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Buttonpi 70% ethanol extract (solid content 1.5% ) 6.0 6. pH adjuster proper amount 7. Preservative proper amount 8. Purified water 100 residue

(Formulation Example 7) Hair liquid weight% 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. A: Trichoderma lucidum 30% ethanol extract ( Solid content 1.0%) B: Botanpi 50% 1,3-butylene glycol extract (solid content 1.0%) C: Perilla 50% ethanol extract (solid content 0.8%) D: Arnica 50% propylene glycol extract (solid content) 1.8%) * Any one of A to D extract 3.0 6. Preservative proper amount 7. Purified water 100 remainder

(Formulation Example 8) Heartonic weight% 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. Crassulaceae extract (solid content 1.8%) 5.0 (ethanol: 1 , 3-butylene glycol = 1: 1 extract) 5. Residue to be purified water 100

(Formulation Example 9) Granule bath agent wt% 1. Sodium hydrogencarbonate 60.0 2. Anhydrous sodium sulfate 32.0 3. Borax 3.0 4. A: Triticum aureus 30% ethanol extract (solid content 1.0%) B: Button pie 50 % 1,3-Vetylene glycol extract (solid content 1.0%) C: Perilla 50% ethanol extract (solid content 0.8%) D: Arnica 50% propylene glycol extract (solid content 1.8%) * A-D 1 kind of extract 5.0

(Formulation Example 10) Coated protective agent A gauze or liniment cloth is impregnated with a formulation solution prepared by mixing an appropriate amount of 30% ethanol extract (solid content 2.0%), antibiotics, anti-inflammatory, etc., and attached to the wound area. . In addition, a 30% ethanol extract (solid content 2.0%) may be directly sprayed locally and covered with gauze or the like.

(Formulation Example 11) Fruit juice beverage% by weight 1. Glucose liquid sugar 33.0 2. Grapefruit juice 62.0 3. Tricholoma communis hot water extract (solid content 1.2%) 2.5 4. Arnica water extract (solid content 1.5%) 2.0 5. Fragrance 0.5 6. Acidulant Suitable amount

(Formulation Example 12) Gum weight% 1. Menthol micron 31.0 2. Grapefruit flavor 65.0 3. Water pipi extract (solid content 1.0%) 2.0 4. Perilla water extract (solid content 1.0%) 2.0

(Test 9) Use effect test The effect of actually using the external preparation for skin and the bath preparation of the present invention was examined. The test was conducted with 10 panelists, 10 to 2 to 30 years old, who suffer from skin diseases such as dry skin, eczema, urticaria, and atopic dermatitis. This was done by applying an appropriate amount to the face for 2 months. Also, for 10 persons (2 to 10 years old) who have similar skin diseases on the scalp and hairline, apply an appropriate amount of the hair artic formula of Prescription Example 8 to the scalp for 2 months after washing the hair daily. The usage test was carried out by.

Further, regarding the bath agent containing the Tricholoma communis / Portrait / Perilla / Arnica extract of Formulation Example 9,
20 people aged 1 to 65 years old who suffer from skin diseases such as dry skin, eczema, urticaria, atopic dermatitis, etc. were dissolved in a proper amount of bath agent once a day for 2 months. I had them bathe in a bath and conducted a usage test. As a control, emulsion,
From hair tonics and bath agents
The one prepared by the same method except for the perilla / Arnica extract was used. The evaluation method was carried out according to the following criteria, and the results are shown in Table 5 and the numerical values in the table represent the number of people.
No one complained of abnormalities on the skin or scalp during the use period.

[Effect of improving skin (scalp) disease] Effectiveness: Redness and itchiness associated with inflammation such as eczema, dry skin, and rough skin were improved. Somewhat effective: Redness and itching, dry skin, and rough skin due to inflammation such as eczema were slightly improved. Ineffective: No change from before use.

(Test Results) As is clear from the results in Table 5, the use of the external preparations for the skin and the bath preparations containing Crataegus infestans, Peacock Fructus, Perilla, and Arnica extract of the present invention, caused irritation due to eczema, itching, dry skin, and rough skin. A good effect was confirmed for the improvement of skin diseases such as.

[0098]

[Table 5]

[0099]

INDUSTRIAL APPLICABILITY According to the present invention, one or more kinds of plant extracts selected from Tricholoma communis, Pleurotus cornucopiae, Perilla frutescens, Arnica, have an antiallergic action, an antihistamine action, an anticomplement activation action,
It has a hyaluronidase activity inhibitory action, an arachidonic acid metabolic activity inhibitory action, and a contact dermatitis inhibitory action, and is safe for human or animal use internally or externally. Therefore, it can be used as an anti-allergic agent, anti-histamine, anti-complement activator, hyaluronidase activity inhibitor, arachidonic acid metabolic activity inhibitor or contact dermatitis inhibitor, eczema, urticaria, atopic dermatitis, allergic rhinitis, pollen. It is effective for the prevention and treatment of various allergic diseases such as infectious diseases. Furthermore, TS can be applied to various forms of preparations (medicines, quasi drugs, cosmetics, foods), and when used as a skin external preparation and a bath preparation, allergic skin inflammation (for example, Redness, eczema, edema, swelling, etc.), atopic dermatitis, contact dermatitis, as well as troubled skin such as eczema, rough skin, dry skin, and itching.
It can be used for the purpose of prevention and improvement of the scalp, and can also be applied to oral compositions and foods.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical display location A61K 35/78 AEM A61K 35/78 AEMT A23G 3/00 101 A23G 3/00 101 3/30 3 / 30 A23L 1/30 A23L 1/30 B 2/52 A61K 7/00 K A61K 7/00 U 7/06 7/06 7/075 7/075 7/08 7/08 7/48 7/48 7/50 7/50 C12N 9/99 C12N 9/99 A23L 2/00 F

Claims (7)

[Claims] 1. An extract obtained by using one or more kinds of plants selected from trillium japonicus, pea pie, perilla, and arnica with water, ethanol, 1,3-butylene glycol, propylene glycol, or a mixture of two or more kinds of these. An anti-allergic agent containing a substance. 2. An extract obtained by using one or more plants selected from Tricholoma communis, Pleurotus cornucopiae, perilla, and Arnica using water, ethanol, 1,3-butylene glycol, propylene glycol, or a mixture of two or more thereof. An antihistamine characterized by containing a substance. 3. An extract obtained by using one or more kinds of plants selected from trillium japonicus, pea pie, perilla, and arnica with water, ethanol, 1,3-butylene glycol, propylene glycol, or a mixed solution of two or more kinds thereof. An anti-complement activator containing a substance. 4. An extract obtained by using one or more plants selected from Tricholoma communis, Pleurotus cornucopiae, perilla, and Arnica using water, ethanol, 1,3-butylene glycol, propylene glycol, or a mixture of two or more thereof. A hyaluronidase activity inhibitor characterized by containing a substance. 5. An extraction obtained by using one or more plants selected from Triturium japonicum, pearl pie, perilla, and Arnica using water, ethanol, 1,3-butylene glycol, propylene glycol, or a mixture of two or more thereof. An agent for suppressing arachidonic acid metabolic activity, which comprises a substance. 6. An extract obtained by using one or more kinds of plants selected from trillium japonicus, pea pie, perilla, and arnica with water, ethanol, 1,3-butylene glycol, propylene glycol, or a mixture of two or more kinds of these. A contact dermatitis inhibitor characterized by containing a substance. 7. An anti-allergic agent according to claim 1-6,
An external preparation for skin and a bath preparation, which comprises any one or more of an antihistamine, an anti-complement activator, a hyaluronidase activity inhibitor, an arachidonic acid metabolic activity inhibitor, and a contact dermatitis inhibitor.