LT3963B - Enzymatic product containing mutant proteolytic enzyme, process for selecting proteolytic enzyme, mutant gene, expression vector, procariotic host-strain, process for preparing mutant proteolytic enzyme, detergent composition - Google Patents

Enzymatic product containing mutant proteolytic enzyme, process for selecting proteolytic enzyme, mutant gene, expression vector, procariotic host-strain, process for preparing mutant proteolytic enzyme, detergent composition Download PDF

Info

Publication number: LT3963B
Authority: LT; Lithuania
Prior art keywords: protease; amino acid; mutant; enzyme; enzymatic product
Prior art date: 1988-02-11

Application number

LTIP1650A

Other languages

English (en)

Lithuanian (lt)

Inventor

Christiaan Albertus Vaneekelen

Leonardus Johannes S Mulleners

Johannes Cornelis Vanderlaan

Onno Misset

Roelck Anneke Cuperus

Johan Herman Albert Lensink

Original Assignee

Gist Brocades Nv

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-02-11

Filing date

1993-12-21

Publication date

1996-05-27

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8199752&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=LT3963(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1993-12-21 Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv

1995-07-25 Publication of LTIP1650A publication Critical patent/LTIP1650A/xx

1996-05-27 Publication of LT3963B publication Critical patent/LT3963B/lt

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NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 description 1
229910052911 sodium silicate Inorganic materials 0.000 description 1
229910052938 sodium sulfate Inorganic materials 0.000 description 1
235000011152 sodium sulphate Nutrition 0.000 description 1
239000004328 sodium tetraborate Substances 0.000 description 1
235000010339 sodium tetraborate Nutrition 0.000 description 1
239000002904 solvent Substances 0.000 description 1
239000002594 sorbent Substances 0.000 description 1
239000000600 sorbitol Substances 0.000 description 1
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YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
239000012137 tryptone Substances 0.000 description 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
Oil, Petroleum & Natural Gas (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Detergent Compositions (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

LTIP1650A 1988-02-11 1993-12-21 Enzymatic product containing mutant proteolytic enzyme, process for selecting proteolytic enzyme, mutant gene, expression vector, procariotic host-strain, process for preparing mutant proteolytic enzyme, detergent composition LT3963B (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
EP88200255		1988-02-11

Publications (2)

Publication Number	Publication Date
LTIP1650A LTIP1650A (en)	1995-07-25
LT3963B true LT3963B (en)	1996-05-27

Family

ID=8199752

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
LTIP1650A LT3963B (en)	1988-02-11	1993-12-21	Enzymatic product containing mutant proteolytic enzyme, process for selecting proteolytic enzyme, mutant gene, expression vector, procariotic host-strain, process for preparing mutant proteolytic enzyme, detergent composition

Country Status (21)

Country	Link
US (1)	US5336611A (de)
EP (3)	EP1160327A3 (de)
JP (1)	JP2849141B2 (de)
KR (1)	KR970005249B1 (de)
CN (1)	CN1056187C (de)
AT (1)	ATE250669T1 (de)
AU (1)	AU629814B2 (de)
BG (1)	BG60566B1 (de)
BR (1)	BR8905580A (de)
CA (1)	CA1340366C (de)
DE (2)	DE68912359T3 (de)
DK (1)	DK175813B1 (de)
ES (1)	ES2061929T5 (de)
FI (1)	FI105481B (de)
GR (1)	GR3021934T3 (de)
IE (2)	IE940164L (de)
LT (1)	LT3963B (de)
LV (1)	LV10652B (de)
PT (1)	PT89702B (de)
RU (1)	RU2069695C1 (de)
WO (1)	WO1989007642A1 (de)

Families Citing this family (127)

* Cited by examiner, † Cited by third party
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US4760025A (en) *	1984-05-29	1988-07-26	Genencor, Inc.	Modified enzymes and methods for making same
US5185258A (en) †	1984-05-29	1993-02-09	Genencor International, Inc.	Subtilisin mutants
EP0309550B1 (de) *	1987-04-03	1996-06-19	Amgen Inc.	Proteolytische enzyme
US6287841B1 (en)	1988-02-11	2001-09-11	Genencor International, Inc.	High alkaline serine protease
ES2227508T3 (es) *	1989-06-26	2005-04-01	Unilever N.V.	Composiciones detergentes enzimaticas.
US5665587A (en) *	1989-06-26	1997-09-09	Novo Nordisk A/S	Modified subtilisins and detergent compositions containing same
DK0414297T3 (da) *	1989-08-11	1997-07-07	Genencor Int	Effektiv fremstilling af mutantproteaser
DE4023458A1 (de) *	1989-08-31	1991-03-07	Kali Chemie Ag	Neue hochalkalische proteasen
US5352603A (en) *	1989-08-31	1994-10-04	Kali-Chemie Ag	Highly alkaline proteases
US6271012B1 (en) *	1989-10-11	2001-08-07	Genencor International, Inc.	Protease muteins and their use in detergents
DK97190D0 (da) *	1990-04-19	1990-04-19	Novo Nordisk As	Oxidationsstabile detergentenzymer
US5733473A (en) *	1990-11-14	1998-03-31	The Procter & Gamble Company	Liquid detergent composition containing lipase and protease
DK0486073T3 (da) *	1990-11-14	1996-12-09	Procter & Gamble	Væskeformig detergentsammensætning, der indeholder lipase og protease
DK271490D0 (da) *	1990-11-14	1990-11-14	Novo Nordisk As	Detergentkomposition
US5482849A (en) *	1990-12-21	1996-01-09	Novo Nordisk A/S	Subtilisin mutants
GB9027836D0 (en) *	1990-12-21	1991-02-13	Unilever Plc	Enzymes and enzymatic detergent compositions
DE4106525A1 (de) *	1991-03-01	1992-09-10	Solvay Enzymes Gmbh & Co Kg	Verfahren zur verbesserung der stabilitaet von hochalkalischen proteasen
US5863783A (en) *	1991-03-27	1999-01-26	Gist-Brocades, N.V.	Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
DK58391D0 (da) *	1991-04-03	1991-04-03	Novo Nordisk As	Hidtil ukendte proteaser
ES2121014T3 (es) *	1991-05-01	1998-11-16	Novo Nordisk As	Enzimas estabilizadas y composiciones detergentes.
US5340735A (en)	1991-05-29	1994-08-23	Cognis, Inc.	Bacillus lentus alkaline protease variants with increased stability
US5510048A (en) *	1991-05-31	1996-04-23	Colgate Palmolive Co.	Nonaqueous liquid, phosphate-free, improved autoamatic dishwashing composition containing enzymes
EP0995801A1 (de) *	1991-07-27	2000-04-26	Genencor International GmbH	Verfahren zur Verbesserung des Stabilität von Enzymen und stabilisierte Enzyme
AT397390B (de) *	1992-04-06	1994-03-25	Immuno Ag	Verfahren zur spaltung von proteinen
US5792623A (en) *	1992-04-06	1998-08-11	Immuno Aktiengesellschaft	Method for producing activated blood factors with a protease and a detergent
EP0571014B1 (de) *	1992-05-18	2004-03-31	Genencor International, Inc.	Alkalische Proteasen produzierende Bakterien, und Verfahren zur Herstellung dieser alkalischen Proteasen
AU682314B2 (en) *	1992-07-17	1997-10-02	Genencor International, Inc.	High alkaline serine proteases
KR100294361B1 (ko) *	1992-07-23	2001-09-17	피아 스타르	돌연변이체알파-아밀라제,세정제,접시세척제,및액화제
US5989887A (en) *	1992-11-25	1999-11-23	Dsm N.V.	Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin
DE4304161A1 (de) *	1993-02-12	1994-08-25	Solvay Enzymes Gmbh & Co Kg	Verbesserte hochalkalische Proteasen
US6440717B1 (en)	1993-09-15	2002-08-27	The Procter & Gamble Company	BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) *	1993-09-15	2002-08-20	The Procter & Gamble Company	BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
DE69434962T2 (de) *	1993-10-14	2008-01-17	The Procter & Gamble Company, Cincinnati	Proteasehaltige reinigungsmittel
US5932532A (en) *	1993-10-14	1999-08-03	Procter & Gamble Company	Bleach compositions comprising protease enzyme
ES2199978T3 (es) *	1993-10-14	2004-03-01	THE PROCTER & GAMBLE COMPANY	Composiciones de blanqueo que comprenden enzimas proteasa.
MA23346A1 (fr)	1993-10-14	1995-04-01	Genencor Int	Variantes de la subtilisine
ES2364774T3 (es)	1994-02-24	2011-09-14	HENKEL AG & CO. KGAA	Enzimas mejoradas y detergentes que las contienen.
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Also Published As

Publication number	Publication date
LV10652A (lv)	1995-04-20
KR900700597A (ko)	1990-08-16
PT89702B (pt)	1994-04-29
EP0571049B2 (de)	2008-09-03
AU3050189A (en)	1989-09-06
CN1056187C (zh)	2000-09-06
EP1160327A3 (de)	2002-11-06
FI894799A0 (fi)	1989-10-10
BR8905580A (pt)	1990-08-14
ATE250669T1 (de)	2003-10-15
DE68912359T3 (de)	1998-01-08
CA1340366C (en)	1999-02-02
DE68929484T3 (de)	2009-06-25
DK501289D0 (da)	1989-10-10
DE68929484T2 (de)	2004-07-22
LV10652B (en)	1995-08-20
IE940164L (en)	1989-08-11
EP0571049B1 (de)	2003-09-24
DK175813B1 (da)	2005-03-07
US5336611A (en)	1994-08-09
BG89942A (bg)	1993-12-24
EP0328229B1 (de)	1994-01-19
WO1989007642A1 (en)	1989-08-24
KR970005249B1 (ko)	1997-04-14
DE68929484D1 (de)	2003-10-30
EP1160327A2 (de)	2001-12-05
DK501289A (da)	1989-12-07
BG60566B1 (en)	1995-08-28
EP0328229B2 (de)	1996-10-30
EP0571049A1 (de)	1993-11-24
JP2849141B2 (ja)	1999-01-20
AU629814B2 (en)	1992-10-15
ES2061929T5 (es)	1997-03-16
CN1036602A (zh)	1989-10-25
DE68912359D1 (de)	1994-03-03
ES2061929T3 (es)	1994-12-16
EP0328229A1 (de)	1989-08-16
LTIP1650A (en)	1995-07-25
RU2069695C1 (ru)	1996-11-27
IE890446L (en)	1989-08-11
IE63248B1 (en)	1995-04-05
JPH02503986A (ja)	1990-11-22
FI105481B (fi)	2000-08-31
GR3021934T3 (en)	1997-03-31
PT89702A (pt)	1989-10-04
DE68912359T2 (de)	1994-06-09

Legal Events

Date	Code	Title	Description
1996-10-25	PC9A	Transfer of patents	Free format text: GENENCOR INTERNATIONAL INC., 4 CAMDRIDGE PLACE, 1870 SOUTH WINTON ROAD, ROCHESTER NY 14618,US,960826
1998-08-25	MM9A	Lapsed patents	Effective date: 19971221

Publication	Publication Date	Title
RU2069695C1 (ru)	1996-11-27	Способ получения мутантной протеазы
US5324653A (en)	1994-06-28	Recombinant genetic means for the production of serine protease muteins
US6271012B1 (en)	2001-08-07	Protease muteins and their use in detergents
US6808913B2 (en)	2004-10-26	Useful mutations of bacterial alkaline protease
DE69839076T2 (de)	2009-01-22	Proteasevarianten und zusammensetzungen
DE69535736T2 (de)	2009-04-30	Verbesserte enzyme und diese enthaltene detergentien
JPH09512433A (ja)	1997-12-16	ズブチリシンｂｐｎ′変異体を含む布帛クリーニング組成物
DK175855B1 (da)	2005-04-04	Mutantprotease og protease til anvendelse i vaskemidler, DNA-sekvens, der koder for en sådan protease, ekspressionsvektor, der omfatter denne DNA-sekvens, prokaryotisk værtsstamme transformeret med en sådan vektor, fremgangsmåde til.....
KR20010093165A (ko)	2001-10-27	활성부위 루프 영역에 추가적 아미노산 잔기를 가지는서브그룹 ｉ-ｓ1과 ｉ-ｓ2의 서브틸라제 효소
MXPA99004150A (en)	1999-09-01	Subtilase variants and compositions