NO175640B - - Google Patents

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Publication number: NO175640B
Authority: NO; Norway
Prior art keywords: sequence; amino acids; plasmid; dna; fusion protein
Prior art date: 1985-07-27

Application number

NO863000A

Other languages

English (en)

Norwegian (no)

Other versions

NO175640C (no

NO863000L (no

NO863000D0 (no

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-07-27

Filing date

1986-07-25

Publication date

1994-08-01

1986-07-25 Application filed filed Critical

1986-07-25 Publication of NO863000D0 publication Critical patent/NO863000D0/no

1987-01-28 Publication of NO863000L publication Critical patent/NO863000L/no

1994-08-01 Publication of NO175640B publication Critical patent/NO175640B/no

1994-11-09 Publication of NO175640C publication Critical patent/NO175640C/no

Links

150000001413 amino acids Chemical class 0.000 claims abstract description 61
102000037865 fusion proteins Human genes 0.000 claims abstract description 42
108020001507 fusion proteins Proteins 0.000 claims abstract description 42
241000588724 Escherichia coli Species 0.000 claims abstract description 19
125000003275 alpha amino acid group Chemical group 0.000 claims abstract 5
108020004414 DNA Proteins 0.000 claims description 40
238000003776 cleavage reaction Methods 0.000 claims description 19
230000007017 scission Effects 0.000 claims description 19
108010076181 Proinsulin Proteins 0.000 claims description 12
108091028043 Nucleic acid sequence Proteins 0.000 claims description 11
239000002773 nucleotide Substances 0.000 claims description 8
125000003729 nucleotide group Chemical group 0.000 claims description 8
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WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 claims description 7
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125000003277 amino group Chemical group 0.000 description 1
HOPRXXXSABQWAV-UHFFFAOYSA-N anhydrous collidine Natural products CC1=CC=NC(C)=C1C HOPRXXXSABQWAV-UHFFFAOYSA-N 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
239000000872 buffer Substances 0.000 description 1
239000006227 byproduct Substances 0.000 description 1
210000004899 c-terminal region Anatomy 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
239000000460 chlorine Substances 0.000 description 1
229910052801 chlorine Inorganic materials 0.000 description 1
230000002759 chromosomal effect Effects 0.000 description 1
UTBIMNXEDGNJFE-UHFFFAOYSA-N collidine Natural products CC1=CC=C(C)C(C)=N1 UTBIMNXEDGNJFE-UHFFFAOYSA-N 0.000 description 1
238000004891 communication Methods 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
239000000470 constituent Substances 0.000 description 1
239000005289 controlled pore glass Substances 0.000 description 1
239000012043 crude product Substances 0.000 description 1
229940104302 cytosine Drugs 0.000 description 1
238000006642 detritylation reaction Methods 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
238000011049 filling Methods 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
239000010931 gold Substances 0.000 description 1
229910052737 gold Inorganic materials 0.000 description 1
230000000415 inactivating effect Effects 0.000 description 1
229910052740 iodine Inorganic materials 0.000 description 1
239000011630 iodine Substances 0.000 description 1
108010064235 lysylglycine Proteins 0.000 description 1
210000004962 mammalian cell Anatomy 0.000 description 1
239000000463 material Substances 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
CAAULPUQFIIOTL-UHFFFAOYSA-N methyl dihydrogen phosphate Chemical group COP(O)(O)=O CAAULPUQFIIOTL-UHFFFAOYSA-N 0.000 description 1
244000005700 microbiome Species 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
PSHKMPUSSFXUIA-UHFFFAOYSA-N n,n-dimethylpyridin-2-amine Chemical compound CN(C)C1=CC=CC=N1 PSHKMPUSSFXUIA-UHFFFAOYSA-N 0.000 description 1
238000000424 optical density measurement Methods 0.000 description 1
125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
239000002243 precursor Substances 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
238000004904 shortening Methods 0.000 description 1
239000001488 sodium phosphate Substances 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
239000012064 sodium phosphate buffer Substances 0.000 description 1
239000007787 solid Substances 0.000 description 1
238000003756 stirring Methods 0.000 description 1
GFYHSKONPJXCDE-UHFFFAOYSA-N sym-collidine Natural products CC1=CN=C(C)C(C)=C1 GFYHSKONPJXCDE-UHFFFAOYSA-N 0.000 description 1
125000001302 tertiary amino group Chemical group 0.000 description 1
229960002180 tetracycline Drugs 0.000 description 1
229930101283 tetracycline Natural products 0.000 description 1
235000019364 tetracycline Nutrition 0.000 description 1
150000003522 tetracyclines Chemical class 0.000 description 1
150000003536 tetrazoles Chemical class 0.000 description 1
229940113082 thymine Drugs 0.000 description 1
238000013518 transcription Methods 0.000 description 1
230000035897 transcription Effects 0.000 description 1
238000013519 translation Methods 0.000 description 1
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
239000001226 triphosphate Substances 0.000 description 1
235000011178 triphosphate Nutrition 0.000 description 1
125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
101150100816 trpD gene Proteins 0.000 description 1
101150079930 trpGD gene Proteins 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Microbiology (AREA)
Gastroenterology & Hepatology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Toxicology (AREA)
Diabetes (AREA)
Endocrinology (AREA)
Tropical Medicine & Parasitology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Saccharide Compounds (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Investigating Or Analysing Biological Materials (AREA)

NO863000A 1985-07-27 1986-07-25 Fusjonsproteiner og DNA som koder for disse NO175640C (no)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE19853526995 DE3526995A1 (de)	1985-07-27	1985-07-27	Fusionsproteine, verfahren zu ihrer herstellung und ihre verwendung

Publications (4)

Publication Number	Publication Date
NO863000D0 NO863000D0 (no)	1986-07-25
NO863000L NO863000L (no)	1987-01-28
NO175640B true NO175640B (pt)	1994-08-01
NO175640C NO175640C (no)	1994-11-09

Family

ID=6276985

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO863000A NO175640C (no)	1985-07-27	1986-07-25	Fusjonsproteiner og DNA som koder for disse

Country Status (18)

Country	Link
EP (1)	EP0211299B1 (pt)
JP (1)	JPH07113040B2 (pt)
KR (1)	KR950000299B1 (pt)
AT (1)	ATE50596T1 (pt)
AU (1)	AU595221B2 (pt)
CA (1)	CA1336329C (pt)
DE (2)	DE3526995A1 (pt)
DK (1)	DK172618B1 (pt)
ES (1)	ES2000278A6 (pt)
FI (1)	FI86887C (pt)
GR (1)	GR861957B (pt)
HU (1)	HU197356B (pt)
IE (1)	IE59127B1 (pt)
IL (1)	IL79522A (pt)
NO (1)	NO175640C (pt)
NZ (1)	NZ216967A (pt)
PT (1)	PT83065B (pt)
ZA (1)	ZA865556B (pt)

Families Citing this family (29)

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DE3738541A1 (de) *	1987-11-13	1989-05-24	Hoechst Ag	Verfahren zur isolierung und reinigung von hirudin
DE3541856A1 (de) *	1985-11-27	1987-06-04	Hoechst Ag	Eukaryotische fusionsproteine, ihre herstellung und verwendung sowie mittel zur durchfuehrung des verfahrens
JPS62220192A (ja) *	1986-03-12	1987-09-28	インタ−ナシヨナル・ミネラルズ・アンド・ケミカル・コ−ポレイシヨン	ハイブリツド表現ベクタ−、その構造および使用
FI896261A7 (fi) *	1987-06-24	1989-12-22	Novo Nordisk As	Menetelmä proteiinin tai polypeptidin valmistamiseksi, polypeptidiä ko odaava DNA-sekvenssi, DNA-sekvenssin sisältämä mikro-organismi sekä po lypeptidi ja sen käyttö farmaseuttisena valmisteena
US5179196A (en) *	1989-05-04	1993-01-12	Sri International	Purification of proteins employing ctap-iii fusions
IL95495A (en) *	1989-08-29	1996-10-16	Hoechst Ag	Fusion proteins their preparation and use
US5227293A (en) *	1989-08-29	1993-07-13	The General Hospital Corporation	Fusion proteins, their preparation and use
US5358857A (en) *	1989-08-29	1994-10-25	The General Hospital Corp.	Method of preparing fusion proteins
GB8927722D0 (en) *	1989-12-07	1990-02-07	British Bio Technology	Proteins and nucleic acids
DE3942580A1 (de) *	1989-12-22	1991-06-27	Basf Ag	Verfahren zur herstellung von hirudin
DE59711533D1 (de)	1996-07-26	2004-05-27	Aventis Pharma Gmbh	Insulinderivate mit erhöhter Zinkbindung
DE19726167B4 (de)	1997-06-20	2008-01-24	Sanofi-Aventis Deutschland Gmbh	Insulin, Verfahren zu seiner Herstellung und es enthaltende pharmazeutische Zubereitung
DE19825447A1 (de)	1998-06-06	1999-12-09	Hoechst Marion Roussel De Gmbh	Neue Insulinanaloga mit erhöhter Zinkbildung
DE10114178A1 (de)	2001-03-23	2002-10-10	Aventis Pharma Gmbh	Zinkfreie und zinkarme Insulinzubereitungen mit verbesserter Stabilität
DE10227232A1 (de)	2002-06-18	2004-01-15	Aventis Pharma Deutschland Gmbh	Saure Insulinzubereitungen mit verbesserter Stabilität
DK2201120T3 (en)	2007-09-21	2014-03-17	Cadila Healthcare Ltd	GCSF fusion protein systems which are suitable for high expression of the peptides
PT3228320T (pt)	2008-10-17	2020-03-26	Sanofi Aventis Deutschland	Combinação de uma insulina e de um agonista do glp-1
KR101853606B1 (ko)	2009-07-06	2018-05-03	사노피-아벤티스 도이칠란트 게엠베하	메티오닌을 함유하는 수성 인슐린 제제
DK2498802T3 (en)	2009-11-13	2015-04-13	Sanofi Aventis Deutschland	Pharmaceutical composition comprising a GLP-1 agonist, insulin and a methionine
KR101337322B1 (ko)	2009-11-13	2013-12-06	사노피-아벤티스 도이칠란트 게엠베하	Ｇｌｐ-1 효능제 및 메티오닌을 포함하는 약제학적 조성물
ES2606554T3 (es)	2010-08-30	2017-03-24	Sanofi-Aventis Deutschland Gmbh	Uso de AVE0010 para la fabricación de un medicamento para el tratamiento de la diabetes mellitus de tipo 2
US9821032B2 (en)	2011-05-13	2017-11-21	Sanofi-Aventis Deutschland Gmbh	Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
PL2750699T3 (pl)	2011-08-29	2015-12-31	Sanofi Aventis Deutschland	Kombinacja farmaceutyczna do stosowania w kontroli glikemii u pacjentów z cukrzycą typu 2
AR087744A1 (es)	2011-09-01	2014-04-16	Sanofi Aventis Deutschland	Composicion farmaceutica para uso en el tratamiento de una enfermedad neurodegenerativa
AU2015359376B2 (en)	2014-12-12	2021-09-09	Sanofi-Aventis Deutschland Gmbh	Insulin glargine/lixisenatide fixed ratio formulation
TWI748945B (zh)	2015-03-13	2021-12-11	德商賽諾菲阿凡提斯德意志有限公司	第２型糖尿病病患治療
TW201705975A (zh)	2015-03-18	2017-02-16	賽諾菲阿凡提斯德意志有限公司	第２型糖尿病病患之治療
KR20200080748A (ko)	2018-12-27	2020-07-07	주식회사 폴루스	음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법
KR20200080747A (ko)	2018-12-27	2020-07-07	주식회사 폴루스	인슐린 전구체의 인슐린 효소 전환용 조성물 및 이를 이용하여 인슐린 전구체를 인슐린으로 전환하는 방법

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Publication number	Priority date	Publication date	Assignee	Title
ZA802992B (en) *	1979-06-01	1981-10-28	Univ California	Human pre-growth hormone
ZA811368B (en) *	1980-03-24	1982-04-28	Genentech Inc	Bacterial polypedtide expression employing tryptophan promoter-operator
WO1986005513A1 (en) *	1985-03-18	1986-09-25	Gene Labs, Inc.	Hybrid-gene cassette vector
GB8507666D0 (en) *	1985-03-25	1985-05-01	Wellcome Found	Epidermal growth factor production
WO1986006099A1 (en) *	1985-04-08	1986-10-23	Genetic Systems Corporation	EXPRESSION AND DIAGNOSTIC USE OF gag ENCODED PEPTIDES WHICH ARE IMMUNOLOGICALLY REACTIVE WITH ANTIBODIES TO LAV

1985
- 1985-07-27 DE DE19853526995 patent/DE3526995A1/de not_active Withdrawn
1986
- 1986-07-19 AT AT86109945T patent/ATE50596T1/de active
- 1986-07-19 DE DE8686109945T patent/DE3669175D1/de not_active Expired - Lifetime
- 1986-07-19 EP EP86109945A patent/EP0211299B1/de not_active Expired - Lifetime
- 1986-07-24 ES ES8600544A patent/ES2000278A6/es not_active Expired
- 1986-07-24 FI FI863041A patent/FI86887C/fi not_active IP Right Cessation
- 1986-07-25 HU HU863100A patent/HU197356B/hu unknown
- 1986-07-25 ZA ZA865556A patent/ZA865556B/xx unknown
- 1986-07-25 NO NO863000A patent/NO175640C/no unknown
- 1986-07-25 CA CA000514682A patent/CA1336329C/en not_active Expired - Lifetime
- 1986-07-25 DK DK198603554A patent/DK172618B1/da not_active IP Right Cessation
- 1986-07-25 NZ NZ216967A patent/NZ216967A/xx unknown
- 1986-07-25 IE IE197786A patent/IE59127B1/en not_active IP Right Cessation
- 1986-07-25 GR GR861957A patent/GR861957B/el unknown
- 1986-07-25 PT PT83065A patent/PT83065B/pt unknown
- 1986-07-25 AU AU60565/86A patent/AU595221B2/en not_active Expired
- 1986-07-25 IL IL79522A patent/IL79522A/xx not_active IP Right Cessation
- 1986-07-26 KR KR1019860006142A patent/KR950000299B1/ko not_active Expired - Lifetime
- 1986-07-26 JP JP61176558A patent/JPH07113040B2/ja not_active Expired - Lifetime

Also Published As

Publication number	Publication date
NO175640C (no)	1994-11-09
FI86887C (fi)	1992-10-26
DK355486A (da)	1987-01-28
JPH07113040B2 (ja)	1995-12-06
DK355486D0 (da)	1986-07-25
DE3526995A1 (de)	1987-02-05
NZ216967A (en)	1988-08-30
IE59127B1 (en)	1994-01-12
PT83065A (de)	1986-08-01
DK172618B1 (da)	1999-03-01
JPS6229600A (ja)	1987-02-07
HU197356B (en)	1989-03-28
CA1336329C (en)	1995-07-18
ZA865556B (en)	1987-03-25
KR870001312A (ko)	1987-03-13
NO863000L (no)	1987-01-28
EP0211299B1 (de)	1990-02-28
IL79522A0 (en)	1986-10-31
ATE50596T1 (de)	1990-03-15
IL79522A (en)	1991-08-16
FI863041L (fi)	1987-01-28
IE861977L (en)	1987-01-27
GR861957B (en)	1986-11-25
FI863041A0 (fi)	1986-07-24
KR950000299B1 (ko)	1995-01-13
EP0211299A2 (de)	1987-02-25
HUT43629A (en)	1987-11-30
NO863000D0 (no)	1986-07-25
DE3669175D1 (de)	1990-04-05
EP0211299A3 (en)	1988-06-01
FI86887B (fi)	1992-07-15
PT83065B (pt)	1989-02-28
ES2000278A6 (es)	1988-02-01
AU595221B2 (en)	1990-03-29
AU6056586A (en)	1987-01-29

Publication	Publication Date	Title
DK172618B1 (da)	1999-03-01	Fusionsproteiner, deres fremstilling og anvendelse
IL95495A (en)	1996-10-16	Fusion proteins their preparation and use
KR950000300B1 (ko)	1995-01-13	융합 단백질의 제조방법
KR100858009B1 (ko)	2008-09-11	세균 배양물의 상층액으로 목적 단백질의 분비를 위한 융합 단백질
FI91883C (fi)	1994-08-25	Menetelmä hirudiinin vaikutusta omaavan polypeptidin valmistamiseksi
DK168823B1 (da)	1994-06-20	Fusionsproteiner, hvor ballastandelen består af peptider af interleukin 2, fremgangsmåde til deres fremstilling, deres anvendelse, genstruktur, der koder for et sådant fusionsprotein, vektor indeholdende en sådan genstruktur, og E. coli-celle indeholdende en sådan vektor.
IE63991B1 (en)	1995-06-28	Novel fusion proteins and their purification
IL74626A (en)	1999-10-28	Insulin-like growth factor i
US7202059B2 (en)	2007-04-10	Fusion proteins capable of being secreted into a fermentation medium
Sung et al.	1986	Short synthetic oligodeoxyribonucleotide leader sequences enhance accumulation of human proinsulin synthesized in Escherichia coli.
KR20010042383A (ko)	2001-05-25	분자내 샤퍼론 유사 서열을 함유하는 키메라 단백질 및이것의 인슐린 제조용 용도
EP0477200A1 (en)	1992-04-01	Purification of proteins employing ctap-iii fusions
Nishi et al.	1983	Construction and application of a novel plasmid" ATG vector" for direct expression of foreign genes in Escherichia coli
KR970001235B1 (ko)	1997-02-04	스트렙토마이세테스(Streptomycetes)에서 외래성 단백질을 제조하는 방법
Del Solar et al.	1994	Chemical synthesis of a fully active transcriptional repressor protein.
Miyashita et al.	1988	High level production of a peptide hormone analogue [Leu13] motilin in E. coli
Murata et al.	1993	Production of poly (L-aspartyl-L-phenylalanine) in Escherichia coli
AU600229B2 (en)	1990-08-09	Modification of the DNA sequemce between the shine-dalgarno sequence and the start codon of the TRP operon to increase protein expression
Verschoor et al.	1989	Cloning, expression and release of native and mutant cloacin DF13 immunity protein
FI97239B (fi)	1996-07-31	Menetelmä fuusioproteiinin valmistamiseksi