NO20000575L - Rekombinant ekspresjon av insulin C-peptid - Google Patents

Rekombinant ekspresjon av insulin C-peptid

Info

Publication number: NO20000575L
Authority: NO; Norway
Prior art keywords: insulin; peptide; recombinant expression; polypeptide; multimeric
Prior art date: 1997-08-07

Application number

NO20000575A

Other languages

English (en)

Norwegian (no)

Other versions

NO20000575D0 (no

Inventor

Stefan Stohl

Mathias Uhlen

Per-Ke Nygren

Per Jonasson

Original Assignee

Creative Peptides Sweden Ab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1997-08-07

Filing date

2000-02-04

Publication date

2000-04-04

2000-02-04 Application filed by Creative Peptides Sweden Ab filed Critical Creative Peptides Sweden Ab

2000-02-04 Publication of NO20000575D0 publication Critical patent/NO20000575D0/no

2000-04-04 Publication of NO20000575L publication Critical patent/NO20000575L/no

Links

VOUAQYXWVJDEQY-QENPJCQMSA-N 33017-11-7 Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N1[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)CCC1 VOUAQYXWVJDEQY-QENPJCQMSA-N 0.000 title abstract 5
238000003259 recombinant expression Methods 0.000 title 1
229920001184 polypeptide Polymers 0.000 abstract 3
108090000765 processed proteins & peptides Proteins 0.000 abstract 3
102000004196 processed proteins & peptides Human genes 0.000 abstract 3
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
238000000034 method Methods 0.000 abstract 2
108010075254 C-Peptide Proteins 0.000 abstract 1
102000004877 Insulin Human genes 0.000 abstract 1
108090001061 Insulin Proteins 0.000 abstract 1
238000003776 cleavage reaction Methods 0.000 abstract 1
239000013604 expression vector Substances 0.000 abstract 1
229940125396 insulin Drugs 0.000 abstract 1
238000004519 manufacturing process Methods 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
230000007017 scission Effects 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Biophysics (AREA)
Diabetes (AREA)
General Engineering & Computer Science (AREA)
Medicinal Chemistry (AREA)
Biotechnology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gastroenterology & Hepatology (AREA)
Endocrinology (AREA)
Physics & Mathematics (AREA)
Toxicology (AREA)
Plant Pathology (AREA)
Microbiology (AREA)
Chemical Kinetics & Catalysis (AREA)
Obesity (AREA)
Public Health (AREA)
Animal Behavior & Ethology (AREA)
Pharmacology & Pharmacy (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
General Chemical & Material Sciences (AREA)
Veterinary Medicine (AREA)
Hematology (AREA)
Emergency Medicine (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

NO20000575A 1997-08-07 2000-02-04 Rekombinant ekspresjon av insulin C-peptid NO20000575L (no)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GBGB9716790.2A GB9716790D0 (en)	1997-08-07	1997-08-07	Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
PCT/GB1998/002382 WO1999007735A2 (en)	1997-08-07	1998-08-07	Recombinant expression of insulin c-peptide

Publications (2)

Publication Number	Publication Date
NO20000575D0 NO20000575D0 (no)	2000-02-04
NO20000575L true NO20000575L (no)	2000-04-04

Family

ID=10817184

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO20000575A NO20000575L (no)	1997-08-07	2000-02-04	Rekombinant ekspresjon av insulin C-peptid

Country Status (21)

Country	Link
US (1)	US6558924B1 (is)
EP (1)	EP1001980A1 (is)
JP (1)	JP2001512745A (is)
KR (1)	KR20010022590A (is)
CN (1)	CN1268141A (is)
AU (1)	AU750685B2 (is)
BG (1)	BG104161A (is)
BR (1)	BR9811850A (is)
CA (1)	CA2297382A1 (is)
EA (1)	EA200000202A1 (is)
EE (1)	EE200000072A (is)
GB (1)	GB9716790D0 (is)
HU (1)	HUP0004626A1 (is)
IL (1)	IL134372A0 (is)
IS (1)	IS5368A (is)
NO (1)	NO20000575L (is)
NZ (1)	NZ502450A (is)
PL (1)	PL339085A1 (is)
SK (1)	SK1532000A3 (is)
TR (1)	TR200000288T2 (is)
WO (1)	WO1999007735A2 (is)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US20040005669A1 (en) *	1997-08-07	2004-01-08	Stefan Stahl	Recombinant expression of insulin C-peptide
FR2810674A1 (fr) *	2000-06-22	2001-12-28	Pf Medicament	Construction modifiee en amont du codon d'initiation pour la surexpression de proteines recombinantes
FR2810675B1 (fr) *	2000-06-22	2002-09-27	Pf Medicament	Construction modifiee en aval du codon d'initiation pour la surexpression de proteines recombinantes
CA2431964C (en) *	2000-12-20	2013-09-10	F. Hoffmann-La Roche Ag	Conjugates of erythropoietin (pep) with polyethylene glycol (peg)
WO2003068934A2 (en) *	2002-02-14	2003-08-21	Rutter William J	Chimeric molecules for cleavage in a treated host
BR0316560A (pt)	2002-11-26	2005-10-04	Nobex Corp	Conjugado de composto natriurético, formulação farmacêutica, método de tratar uma condição, método de preparação do conjugado de composto natriurético, conjugado de peptìdeo pró-polinatriurético modificado, análogo de peptìdeo natriurético, análogo de hbnp, composto, e, método de preparar um composto
US20050026826A1 (en) *	2003-01-17	2005-02-03	Margarethe Hoenig	Feline proinsulin, insulin and constituent peptides
WO2004106525A1 (fr) *	2003-06-03	2004-12-09	Shanghai Centre Of Research & Development Of New Drugs	Proteine de fusion pouvant etre exprimee tres efficacement et son procede de production
CN1930188B (zh) *	2004-03-10	2012-01-11	克赖顿大学	雌激素受体和使用方法
NZ603399A (en)	2010-05-17	2014-09-26	Cebix Inc	Pegylated c-peptide
WO2013075117A2 (en)	2011-11-17	2013-05-23	John Wahren	Pegylated c-peptide
AU2013234860B2 (en) *	2012-03-19	2016-01-21	Madeleine Pharmaceuticals Pty Ltd	Method of producing a recombinant peptide
WO2016145389A1 (en)	2015-03-12	2016-09-15	Board Of Trustees Of Michigan State University	Compositions and methods for measuring c-peptide binding and diagnosing immune-mediated diseases
CN109705221B (zh) *	2018-12-27	2021-03-09	美康生物科技股份有限公司	C肽免疫原及其单克隆抗体对及该抗体对在c肽磁微粒化学发光免疫试剂中的应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
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JPS5210872B2 (is)	1973-07-14	1977-03-26
GB2068971B (en)	1980-01-30	1983-06-08	Searle & Co	Recombinant dna techniques
CA1213537A (en)	1984-05-01	1986-11-04	Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee	Polypeptide expression method
AU605291B2 (en)	1987-03-20	1991-01-10	Micromet Ag	Process for the purification of recombinant polypeptides
US5013653A (en)	1987-03-20	1991-05-07	Creative Biomolecules, Inc.	Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
SE501169C2 (sv)	1988-02-05	1994-11-28	Aake Nygren	Framställning av serumalbuminbindande fusionsproteiner vilka innehåller protein G-domäner
US5130236A (en)	1989-12-07	1992-07-14	Eli Lilly And Company	Process for producing des(64,65)-proinsulin
US5595887A (en)	1990-07-16	1997-01-21	Bionebraska, Inc.	Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
GB9015825D0 (en)	1990-07-18	1990-09-05	Ciba Geigy Ag	In vitro processing of fusion proteins
JPH07108232B2 (ja)	1990-10-09	1995-11-22	エム・ディ・リサーチ株式会社	ペプチド又は蛋白質の製造方法
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WO1997023639A1 (en)	1995-12-22	1997-07-03	Toray Industries, Inc.	Process for producing biologically active fused proteins

1997
- 1997-08-07 GB GBGB9716790.2A patent/GB9716790D0/en active Pending
1998
- 1998-08-07 BR BR9811850-1A patent/BR9811850A/pt not_active Application Discontinuation
- 1998-08-07 SK SK153-2000A patent/SK1532000A3/sk unknown
- 1998-08-07 TR TR2000/00288T patent/TR200000288T2/xx unknown
- 1998-08-07 EP EP98937689A patent/EP1001980A1/en not_active Withdrawn
- 1998-08-07 EA EA200000202A patent/EA200000202A1/ru unknown
- 1998-08-07 NZ NZ502450A patent/NZ502450A/xx unknown
- 1998-08-07 CA CA002297382A patent/CA2297382A1/en not_active Abandoned
- 1998-08-07 WO PCT/GB1998/002382 patent/WO1999007735A2/en not_active Ceased
- 1998-08-07 JP JP2000506237A patent/JP2001512745A/ja active Pending
- 1998-08-07 HU HU0004626A patent/HUP0004626A1/hu unknown
- 1998-08-07 PL PL98339085A patent/PL339085A1/xx not_active Application Discontinuation
- 1998-08-07 US US09/485,286 patent/US6558924B1/en not_active Expired - Fee Related
- 1998-08-07 EE EEP200000072A patent/EE200000072A/xx unknown
- 1998-08-07 IL IL13437298A patent/IL134372A0/xx unknown
- 1998-08-07 KR KR1020007001182A patent/KR20010022590A/ko not_active Withdrawn
- 1998-08-07 CN CN98808075A patent/CN1268141A/zh active Pending
- 1998-08-07 AU AU86407/98A patent/AU750685B2/en not_active Ceased
2000
- 2000-02-01 IS IS5368A patent/IS5368A/is unknown
- 2000-02-04 NO NO20000575A patent/NO20000575L/no not_active Application Discontinuation
- 2000-02-17 BG BG104161A patent/BG104161A/xx unknown

Also Published As

Publication number	Publication date
AU750685B2 (en)	2002-07-25
HUP0004626A1 (hu)	2001-04-28
SK1532000A3 (en)	2000-09-12
PL339085A1 (en)	2000-12-04
NZ502450A (en)	2002-10-25
AU8640798A (en)	1999-03-01
WO1999007735A2 (en)	1999-02-18
BR9811850A (pt)	2000-08-08
GB9716790D0 (en)	1997-10-15
US6558924B1 (en)	2003-05-06
TR200000288T2 (tr)	2000-06-21
EE200000072A (et)	2000-10-16
EA200000202A1 (ru)	2001-04-23
BG104161A (en)	2000-12-29
NO20000575D0 (no)	2000-02-04
IL134372A0 (en)	2001-04-30
JP2001512745A (ja)	2001-08-28
IS5368A (is)	2000-02-01
KR20010022590A (ko)	2001-03-26
WO1999007735A3 (en)	1999-04-29
CN1268141A (zh)	2000-09-27
EP1001980A1 (en)	2000-05-24
CA2297382A1 (en)	1999-02-18

Legal Events

Date	Code	Title	Description
2002-05-21	FC2A	Withdrawal, rejection or dismissal of laid open patent application

Publication	Publication Date	Title
NO20020861L (no)	2002-04-19	Nye B7-4-molekyler og anvendelser derav
BRPI9811940A (pt)	2000-09-05	vetor de expressão para um polipeptídeo de ligação da interleucina-18 (il-18-bp), célula hospedeira procariótica transformada com o referido vetor, processo para produção de il-18-bp, proteína de fusão compreendendo o referido polipeptídeo, bem como il-18bp modificada.
BR9813608B1 (pt)	2011-11-01	célula hospedeira de bacillus transgênica, e, processo para produzir um polipeptìdeo tendo atividade aminopeptidase.
BR0009392A (pt)	2002-01-08	Polipeptìdeo, sequência de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, métodos para produzir uma sequência de ácido nucleico mutante e para produzir um polipeptìdeo, mutante de um polipeptìdeo com atividade de alafa-amilase, e, uso do polipeptìdeo ou variante
WO1994003616A3 (fr)	1994-03-31	Vecteur de clonage et/ou de sequençage
BR9713294A (pt)	2000-10-17	"anticorpos contra proteìna relacionada a hormÈnio de paratiróide humano"
WO1995011984A3 (en)	1995-07-06	Recombinant adenoviral vector and methods of use
KR960010864A (ko)	1996-04-20	동물 세포의 형질 감염을 위한 재조합 dna 바이러스 벡터
PT728214E (pt)	2004-11-30	Linhas celulares estaveis capazes de expressar o gene de replicacao do virus adeno-associado
DE68927456D1 (de)	1996-12-19	RNS-Katalysator zur Spaltung von spezifischen RNS-Sequenzen
EP0776331A4 (en)	1998-11-25	HIGHER ORDER STRUCTURE AND BINDING OF PEPTIDE NUCLEIC ACIDS
EP1798239A3 (en)	2012-08-15	G-protein coupled receptors and uses therefor
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BR9915821A (pt)	2001-10-23	Métodos para transformação de plantas para expressar delta-endotoxinas de bacillus thuringiensis
WO1999007735A3 (en)	1999-04-29	Recombinant expression of insulin c-peptide
IE831211L (en)	1983-11-25	Cloning vectors for expression of exogenous protein
DE69933550D1 (de)	2006-11-23	Modifizierte adenoviren welche ein faserersatzprotein enthalten
WO2004083234A3 (en)	2005-08-04	Insulin polypeptide-oligomer conjugates, proinsulin polypeptide-oligomer conjugates and methods of synthesizing same
MY127575A (en)	2006-12-29	Methods of synthesizing insulin polypeptide-oligomer conjugates, and proinsulin polypeptide-oligomer conjugates and methods of synthesizing same
BR9811865A (pt)	2000-08-15	Isolamento de um novo gene de fator de senescência, p23
ID27206A (id)	2001-03-08	Generasi produk-produk perbanyakan gena dari protein-protein fusi simerik melalui pemutusan endoprotease ubikuitus
AR012499A1 (es)	2000-10-18	Una secuencia quimerica de acido nucleico que codifica una proteina de fusion, un vector de expresion que comprende dicha secuencia quimerica, una celula huesped transformada que contiene dicho vector, la proteina de fusion obtenida y un metodo in vitro para la preparacion de un polipeptido recombin
NO972063L (no)	1997-05-05	Isolert BMP-reseptorkinaseprotein, DNA-sekvens, rekombinant ekspresjonsvektor samt vertscelle
WO2003099854A3 (en)	2007-02-15	Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides
KR950701345A (ko)	1995-03-23	엘라스타제 저해활성을 갖는 신규 펩티드 및 그의 제조방법