NO20021254L - Signalsekvenser for fremstilling av LEU-hirudin via sekresjon fra E. Coli i et dyrkningsmedium - Google Patents

Signalsekvenser for fremstilling av LEU-hirudin via sekresjon fra E. Coli i et dyrkningsmedium

Info

Publication number
NO20021254L
NO20021254L NO20021254A NO20021254A NO20021254L NO 20021254 L NO20021254 L NO 20021254L NO 20021254 A NO20021254 A NO 20021254A NO 20021254 A NO20021254 A NO 20021254A NO 20021254 L NO20021254 L NO 20021254L
Authority
NO
Norway
Prior art keywords
hirudin
leu
coli
preparation
culture medium
Prior art date
Application number
NO20021254A
Other languages
English (en)
Other versions
NO20021254D0 (no
NO329723B1 (no
Inventor
Paul Habermann
Rudolf Bender
Original Assignee
Aventis Pharma Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Gmbh filed Critical Aventis Pharma Gmbh
Publication of NO20021254D0 publication Critical patent/NO20021254D0/no
Publication of NO20021254L publication Critical patent/NO20021254L/no
Publication of NO329723B1 publication Critical patent/NO329723B1/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
NO20021254A 1999-09-18 2002-03-13 Signalsekvenser for fremstilling av LEU-hirudin via sekresjon og anvendelsen av denne NO329723B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19944870A DE19944870A1 (de) 1999-09-18 1999-09-18 Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
PCT/EP2000/008537 WO2001021662A1 (de) 1999-09-18 2000-09-01 Signalsequenzen zur herstellung von leu-hirudin über sekretion durch e. coli in das kulturmedium

Publications (3)

Publication Number Publication Date
NO20021254D0 NO20021254D0 (no) 2002-03-13
NO20021254L true NO20021254L (no) 2002-05-07
NO329723B1 NO329723B1 (no) 2010-12-06

Family

ID=7922547

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20021254A NO329723B1 (no) 1999-09-18 2002-03-13 Signalsekvenser for fremstilling av LEU-hirudin via sekresjon og anvendelsen av denne

Country Status (28)

Country Link
US (1) US7455987B1 (no)
EP (1) EP1216259B1 (no)
JP (1) JP4669181B2 (no)
KR (1) KR100737189B1 (no)
CN (2) CN1269838C (no)
AR (1) AR025660A1 (no)
AT (1) ATE357458T1 (no)
AU (1) AU775923B2 (no)
BR (1) BR0014519A (no)
CA (1) CA2385287C (no)
CZ (1) CZ2002899A3 (no)
DE (2) DE19944870A1 (no)
DK (1) DK1216259T3 (no)
EE (1) EE05187B1 (no)
HR (1) HRP20020226B1 (no)
HU (1) HUP0202596A3 (no)
IL (2) IL148624A0 (no)
ME (1) MEP27908A (no)
MX (1) MXPA02002469A (no)
NO (1) NO329723B1 (no)
NZ (1) NZ517825A (no)
PL (1) PL205117B1 (no)
RS (1) RS50435B (no)
RU (1) RU2261867C2 (no)
SK (1) SK287865B6 (no)
TR (2) TR200501541T2 (no)
WO (1) WO2001021662A1 (no)
ZA (1) ZA200202106B (no)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
US7638618B2 (en) 2001-02-20 2009-12-29 Sanofi-Aventis Deutschland Gmbh Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
AU2004317306B2 (en) * 2003-11-21 2010-09-02 Pelican Technology Holdings, Inc. Improved expression systems with Sec-system secretion
US8603824B2 (en) 2004-07-26 2013-12-10 Pfenex, Inc. Process for improved protein expression by strain engineering
DE102005046113A1 (de) * 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen
DE102006044841A1 (de) * 2006-09-22 2008-04-03 Wacker Chemie Ag Signalpeptid zur Produktion von rekombinanten Proteinen
JP5714230B2 (ja) 2007-01-31 2015-05-07 フェネックス インコーポレイテッド 発現上昇のための細菌リーダー配列
JP5444553B2 (ja) 2007-04-27 2014-03-19 フェネックス インコーポレイテッド 微生物宿主を迅速にスクリーニングして、異種タンパク質発現の収率および／または質が改善されている特定の株を同定する方法
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
ES2853935T3 (es) 2013-03-12 2021-09-20 Massachusetts Gen Hospital Proteínas modificadas de sustancia inhibidora muleriana (MIS) y usos de las mismas para el tratamiento de enfermedades
US11135269B2 (en) 2013-12-11 2021-10-05 The General Hospital Corporation Use of mullerian inhibiting substance (MIS) proteins for contraception and ovarian reserve preservation
CN108220369B (zh) * 2017-12-04 2021-03-12 广东双骏生物科技有限公司 一种生产重组水蛭素的方法
CN108359689B (zh) * 2018-01-19 2021-03-23 华中农业大学 利用抗病基因识别无毒效应蛋白以鉴定具有分泌功能信号肽的方法
CN115572329B (zh) * 2021-06-21 2024-02-06 王大勇 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
ES2055149T3 (es) * 1985-04-11 1994-08-16 Hoechst Ag Derivado de hirudina.
US5084384A (en) * 1987-04-23 1992-01-28 Monsanto Company Secretion of insulin-like growth factor-I
AU604925B2 (en) * 1988-02-23 1991-01-03 Schering Aktiengesellschaft A hirudin derivative
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
DK0687731T3 (da) * 1990-11-08 1999-09-20 Japan Energy Corp Sekretionsvektor, transformerede mikroorganismer indeholdende vektoren og fremstilling af produkter fra denne mikroorganism
JPH07119237B2 (ja) * 1990-11-08 1995-12-20 株式会社ジャパンエナジー ヒルジン変異体，その製造法及び抗凝血剤
US5389529A (en) * 1991-06-12 1995-02-14 Regeneron Pharmaceuticals, Inc. Modified lamβ signal sequence and processes for producing recombinant neurotrophins
PT100580A (pt) * 1991-06-12 1993-09-30 Regeneron Pharma Producao e recuperacao de neurotrofinas recombinantes
DE4140381A1 (de) 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De Neue synthetische isohirudine mit verbesserter stabilitaet

Also Published As

Publication number Publication date
NZ517825A (en) 2004-07-30
MXPA02002469A (es) 2002-08-28
DE50014189D1 (de) 2007-05-03
DK1216259T3 (da) 2007-07-02
RU2002110278A (ru) 2004-03-27
WO2001021662A1 (de) 2001-03-29
BR0014519A (pt) 2002-06-11
EE200200139A (et) 2003-06-16
TR200200667T2 (tr) 2002-06-21
HUP0202596A2 (hu) 2002-12-28
HK1068352A1 (en) 2005-04-29
KR100737189B1 (ko) 2007-07-10
PL205117B1 (pl) 2010-03-31
CN1269838C (zh) 2006-08-16
HUP0202596A3 (en) 2005-01-28
PL354444A1 (en) 2004-01-12
DE19944870A1 (de) 2001-03-29
CN1192037C (zh) 2005-03-09
HRP20020226B1 (hr) 2010-08-31
HK1049172A1 (en) 2003-05-02
ATE357458T1 (de) 2007-04-15
AU6842100A (en) 2001-04-24
CN1550502A (zh) 2004-12-01
YU14602A (sh) 2005-07-19
JP4669181B2 (ja) 2011-04-13
SK3622002A3 (en) 2003-06-03
RS50435B (sr) 2009-12-31
EE05187B1 (et) 2009-06-15
HRP20020226A2 (en) 2004-04-30
NO20021254D0 (no) 2002-03-13
TR200501541T2 (tr) 2005-06-21
EP1216259B1 (de) 2007-03-21
EP1216259A1 (de) 2002-06-26
KR20020034188A (ko) 2002-05-08
CZ2002899A3 (cs) 2002-06-12
ZA200202106B (en) 2003-01-09
NO329723B1 (no) 2010-12-06
CN1374970A (zh) 2002-10-16
MEP27908A (en) 2010-10-10
CA2385287C (en) 2010-10-19
CA2385287A1 (en) 2001-03-29
AR025660A1 (es) 2002-12-11
IL148624A0 (en) 2002-09-12
IL148624A (en) 2009-08-03
JP2003510041A (ja) 2003-03-18
US7455987B1 (en) 2008-11-25
AU775923B2 (en) 2004-08-19
RU2261867C2 (ru) 2005-10-10
SK287865B6 (sk) 2012-02-03

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