NO308619B1 - Rekombinante DNA-molekyler og fremgangsmÕte for fremstilling av heterologt protein - Google Patents

Rekombinante DNA-molekyler og fremgangsmÕte for fremstilling av heterologt protein Download PDF

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Publication number: NO308619B1
Authority: NO; Norway
Prior art keywords: yeast; protein; sequence; host cell; kex2
Prior art date: 1991-12-16

Application number

NO924847A

Other languages

English (en)

Norwegian (no)

Other versions

NO924847L (no

NO924847D0 (no

Inventor

Bhabatosh Chaudhuri

Christine Stephan

Peter Seeboth

Howard Riezman

Original Assignee

Novartis Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-12-16

Filing date

1992-12-15

Publication date

2000-10-02

1992-12-15 Application filed by Novartis Ag filed Critical Novartis Ag

1992-12-15 Publication of NO924847D0 publication Critical patent/NO924847D0/no

1993-06-17 Publication of NO924847L publication Critical patent/NO924847L/no

2000-10-02 Publication of NO308619B1 publication Critical patent/NO308619B1/no

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Mycology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Endocrinology (AREA)
Diabetes (AREA)
Gastroenterology & Hepatology (AREA)
Analytical Chemistry (AREA)
Toxicology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Saccharide Compounds (AREA)
Enzymes And Modification Thereof (AREA)
Peptides Or Proteins (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)

NO924847A 1991-12-16 1992-12-15 Rekombinante DNA-molekyler og fremgangsmÕte for fremstilling av heterologt protein NO308619B1 (no)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
EP91810984		1991-12-16

Publications (3)

Publication Number	Publication Date
NO924847D0 NO924847D0 (no)	1992-12-15
NO924847L NO924847L (no)	1993-06-17
NO308619B1 true NO308619B1 (no)	2000-10-02

Family

ID=8208912

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO924847A NO308619B1 (no)	1991-12-16	1992-12-15	Rekombinante DNA-molekyler og fremgangsmÕte for fremstilling av heterologt protein

Country Status (17)

Country	Link
US (2)	US5501975A (fr)
EP (1)	EP0548012B1 (fr)
JP (1)	JP3305781B2 (fr)
KR (1)	KR100270283B1 (fr)
AT (1)	ATE157703T1 (fr)
AU (1)	AU667852B2 (fr)
CA (1)	CA2085308C (fr)
DE (1)	DE69222013T2 (fr)
DK (1)	DK0548012T3 (fr)
ES (1)	ES2107520T3 (fr)
FI (1)	FI105482B (fr)
GR (1)	GR3025324T3 (fr)
IL (1)	IL104075A (fr)
MX (1)	MX9207291A (fr)
NO (1)	NO308619B1 (fr)
NZ (1)	NZ245469A (fr)
ZA (1)	ZA929711B (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB9404270D0 (en) *	1994-03-05	1994-04-20	Delta Biotechnology Ltd	Yeast strains and modified albumins
EP0812362A1 (fr) *	1995-02-27	1997-12-17	Abbott Laboratories	Plasmide pour l'expression de proteines de recombinaison modifiees dans un systeme bacterien
US5942254A (en) *	1995-02-27	1999-08-24	Abbott Laboratories	Phosphorylated recombinant human β-casein expressed in a bacterial system
EP0812361A1 (fr) *	1995-02-27	1997-12-17	Abbott Laboratories	Methode d'expression de proteines recombinantes modifiees dans un systeme bacterien
US5807702A (en) *	1995-02-27	1998-09-15	Abbott Laboratories	Method for expressing phosphorylated recombinant human β-casein in a bacterial system
US5710044A (en) *	1995-02-27	1998-01-20	Abbott Laboratories	Plasmid for expressing phosphorylated recombinant proteins in a bacterial system
AT404838B (de) *	1995-11-24	1999-03-25	Immuno Ag	Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
FR2751000B1 (fr)	1996-07-12	1998-10-30	Inst Nat Sante Rech Med	Adn specifiques des bacteries de l'espece neisseria meningitidis, leurs procedes d'obtention et leurs applications biologiques
JP4463988B2 (ja) *	1998-11-06	2010-05-19	ノボノルディスクヘルスケアアクチェンゲゼルシャフト	Ｖｉｉ因子の生産方法
EP2028275A3 (fr)	2000-06-30	2009-05-06	VIB vzw	Modification de glycosylation de protéine dans pichia pastoris
DE10221411B4 (de) *	2002-05-14	2004-07-08	GSF-Forschungszentrum für Umwelt und Gesundheit GmbH	Rekombinantes Fowlpox-Virus
US20040117874A1 (en) *	2002-10-15	2004-06-17	Jianjun Yang	Methods for accumulating translocated proteins
US7507573B2 (en)	2003-11-14	2009-03-24	Vib, Vzw	Modification of protein glycosylation in methylotrophic yeast
AU2005323451B2 (en) *	2004-05-21	2010-11-18	Qiagen Sciences Llc	Kits and processes for removing contaminants from nucleic acids in environmental and biological samples
US20080082338A1 (en) *	2006-09-29	2008-04-03	O'neil Michael P	Systems and methods for secure voice identification and medical device interface
RU2344173C2 (ru) *	2006-10-27	2009-01-20	Институт биохимии им. А.Н. Баха Российской Академии Наук	Кассета и рекомбинантная плазмида для зкспрессии и секреции механозависимого фактора роста человека (mgf), штамм saccharomyces cerevisiae - продуцент mgf и способ получения mgf
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1992
- 1992-12-08 AT AT92810964T patent/ATE157703T1/de active
- 1992-12-08 ES ES92810964T patent/ES2107520T3/es not_active Expired - Lifetime
- 1992-12-08 DE DE69222013T patent/DE69222013T2/de not_active Expired - Lifetime
- 1992-12-08 DK DK92810964.4T patent/DK0548012T3/da active
- 1992-12-08 EP EP92810964A patent/EP0548012B1/fr not_active Expired - Lifetime
- 1992-12-10 AU AU30071/92A patent/AU667852B2/en not_active Expired
- 1992-12-13 IL IL10407592A patent/IL104075A/xx not_active IP Right Cessation
- 1992-12-14 KR KR1019920024117A patent/KR100270283B1/ko not_active Expired - Lifetime
- 1992-12-14 CA CA002085308A patent/CA2085308C/fr not_active Expired - Lifetime
- 1992-12-14 FI FI925678A patent/FI105482B/fi not_active IP Right Cessation
- 1992-12-14 NZ NZ245469A patent/NZ245469A/en unknown
- 1992-12-15 ZA ZA929711A patent/ZA929711B/xx unknown
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- 1992-12-15 NO NO924847A patent/NO308619B1/no not_active IP Right Cessation
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1994
- 1994-10-24 US US08/328,961 patent/US5501975A/en not_active Expired - Lifetime
1995
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1997
- 1997-11-07 GR GR970402966T patent/GR3025324T3/el unknown

Also Published As

Publication number	Publication date
JP3305781B2 (ja)	2002-07-24
FI925678L (fi)	1993-06-17
IL104075A (en)	2003-01-12
ES2107520T3 (es)	1997-12-01
GR3025324T3 (en)	1998-02-27
NO924847L (no)	1993-06-17
JPH06197787A (ja)	1994-07-19
NO924847D0 (no)	1992-12-15
DK0548012T3 (da)	1998-04-14
ZA929711B (en)	1993-06-16
AU667852B2 (en)	1996-04-18
ATE157703T1 (de)	1997-09-15
AU3007192A (en)	1993-06-17
US5618690A (en)	1997-04-08
US5501975A (en)	1996-03-26
KR100270283B1 (ko)	2000-11-01
EP0548012B1 (fr)	1997-09-03
FI925678A0 (fi)	1992-12-14
DE69222013D1 (de)	1997-10-09
MX9207291A (es)	1993-07-01
DE69222013T2 (de)	1998-01-29
KR930013115A (ko)	1993-07-21
IL104075A0 (en)	1993-05-13
EP0548012A1 (fr)	1993-06-23
CA2085308A1 (fr)	1993-06-17
CA2085308C (fr)	2003-12-02
NZ245469A (en)	1995-03-28
FI105482B (fi)	2000-08-31

Legal Events

Date	Code	Title	Description
2013-01-14	MK1K	Patent expired

Publication	Publication Date	Title
EP0548012B1 (fr)	1997-09-03	Endoprotéase dibasique recombinante localisée dans le réticulum endoplasmique et ses applications
Parekh et al.	1995	Multicopy overexpression of bovine pancreatic trypsin inhibitor saturates the protein folding and secretory capacity of Saccharomyces cerevisiae
JP5027812B2 (ja)	2012-09-19	トリプシンの変異体によるインスリン前駆体の切断
IL114160A (en)	2006-12-31	Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast
IE66044B1 (en)	1995-12-13	Improvements in the production of polypeptides
NO175871B (fr)	1994-09-12
US6358705B1 (en)	2002-03-19	Method of making proteins in transformed yeast cells
US5521093A (en)	1996-05-28	Yeast vector coding for heterologous gene fusions linked via KEX2 cleavage site and coding for truncated KEX2 genes
JP4180112B2 (ja)	2008-11-12	酵母細胞におけるｎ末端を伸長されたタンパクの発現のためのベクター
US6183989B1 (en)	2001-02-06	Process for making desired polypeptides in yeast
EP1211314A2 (fr)	2002-06-05	Expression d'un précurseur de l'insuline humaine dans p. pastoris
EP0467839B1 (fr)	1996-08-21	Traitement in vitro de protéine fusion
EP1097228B1 (fr)	2006-03-01	Technique de production de proteines dans des cellules de levure transformees
NZ250442A (en)	1995-12-21	Hybrid vectors and yeast cells and their use in the production of polypeptides
RU2167939C2 (ru)	2001-05-27	Способ продуцирования полипептида в дрожжах
KR19990025486A (ko)	1999-04-06	Yap3 유전자가 결손된 신규한 효모균주 및 그를 이용한 재조합 인체 부갑상선호르몬의 생산
HK1124341B (en)	2013-06-14	Cleavage of precursors of insulins by a variant of trypsin