NO310491B1 - Metode samt apparat for kryokonservering - Google Patents

Metode samt apparat for kryokonservering Download PDF

Info

Publication number: NO310491B1
Authority: NO; Norway
Prior art keywords: tissue; temperature; equivalent; cryopreserved; cultured
Prior art date: 1995-01-30

Application number

NO19973463A

Other languages

English (en)

Norwegian (no)

Other versions

NO973463L (no

NO973463D0 (no

Inventor

Stephen R Watson

Mehmet Toner

Alexander G Tschumakow

Original Assignee

Organogenesis Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1995-01-30

Filing date

1997-07-28

Publication date

2001-07-16

1997-07-28 Application filed by Organogenesis Inc filed Critical Organogenesis Inc

1997-07-28 Publication of NO973463D0 publication Critical patent/NO973463D0/no

1997-09-30 Publication of NO973463L publication Critical patent/NO973463L/no

2001-07-16 Publication of NO310491B1 publication Critical patent/NO310491B1/no

Links

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Classifications

- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/14—Mechanical aspects of preservation; Apparatus or containers therefor
- A01N1/146—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/16—Physical preservation processes
- A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D55/00—Accessories for container closures not otherwise provided for
- B65D55/02—Locking devices; Means for discouraging or indicating unauthorised opening or removal of closure
- B65D55/06—Deformable or tearable wires, strings or strips; Use of seals
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D77/00—Packages formed by enclosing articles or materials in preformed containers, e.g. boxes, cartons, sacks or bags
- B65D77/04—Articles or materials enclosed in two or more containers disposed one within another
- B65D77/048—Articles or materials enclosed in two or more containers disposed one within another the inner and outer containers being rigid and the outer container being of curved cross-section, e.g. cylindrical
- B65D77/0486—Articles or materials enclosed in two or more containers disposed one within another the inner and outer containers being rigid and the outer container being of curved cross-section, e.g. cylindrical the inner container being coaxially disposed within the outer container
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D85/00—Containers, packaging elements or packages, specially adapted for particular articles or materials
- B65D85/50—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage

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Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Dentistry (AREA)
General Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Environmental Sciences (AREA)
Mechanical Engineering (AREA)
Agricultural Chemicals And Associated Chemicals (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)
Packging For Living Organisms, Food Or Medicinal Products That Are Sensitive To Environmental Conditiond (AREA)
Packages (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Catching Or Destruction (AREA)
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NO19973463A 1995-01-30 1997-07-28 Metode samt apparat for kryokonservering NO310491B1 (no)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US08/380,099 US5891617A (en)	1993-09-15	1995-01-30	Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing
PCT/US1996/001217 WO1996024018A1 (fr)	1995-01-30	1996-01-30	Procede et structure d'emballage pour la conservation et le stockage cryogeniques d'equivalents de tissu de culture

Publications (3)

Publication Number	Publication Date
NO973463D0 NO973463D0 (no)	1997-07-28
NO973463L NO973463L (no)	1997-09-30
NO310491B1 true NO310491B1 (no)	2001-07-16

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ID=23499896

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO19973463A NO310491B1 (no)	1995-01-30	1997-07-28	Metode samt apparat for kryokonservering

Country Status (21)

Country	Link
US (1)	US5891617A (fr)
EP (2)	EP0807234B1 (fr)
JP (4)	JPH11501298A (fr)
KR (1)	KR100328742B1 (fr)
CN (2)	CN1292651C (fr)
AT (2)	ATE376356T1 (fr)
AU (1)	AU716869B2 (fr)
BR (1)	BR9606864A (fr)
CZ (1)	CZ294950B6 (fr)
DE (2)	DE69633854T2 (fr)
ES (1)	ES2231804T3 (fr)
FI (1)	FI113694B (fr)
HU (1)	HU223789B1 (fr)
NO (1)	NO310491B1 (fr)
NZ (1)	NZ302913A (fr)
PL (1)	PL181762B1 (fr)
PT (1)	PT807234E (fr)
RU (1)	RU2178865C2 (fr)
SG (1)	SG106553A1 (fr)
SK (1)	SK284801B6 (fr)
WO (1)	WO1996024018A1 (fr)

Families Citing this family (96)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5891617A (en) *	1993-09-15	1999-04-06	Organogenesis Inc.	Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing
US5689961A (en)	1996-01-30	1997-11-25	Organogenesis Inc.	Ice seeding apparatus for cryopreservation systems
GB2330516A (en) *	1997-10-22	1999-04-28	Elizabeth Acton	Cryopreservation of cell suspensions
US6291240B1 (en) *	1998-01-29	2001-09-18	Advanced Tissue Sciences, Inc.	Cells or tissues with increased protein factors and methods of making and using same
ES2257050T3 (es)	1998-05-26	2006-07-16	Lifecell Corporation	Conservacion por el frio de hematies humanos.
EP2295540A1 (fr)	1998-11-19	2011-03-16	Organogenesis, Inc.	Constructions de tissu issues de la bioingénierie et leurs procédés de fabrication et d'utilisation
US20010036665A1 (en) *	1999-03-18	2001-11-01	Susan M. Young	Method of preparing cryogenically preserved adherent cell containing plate for tissue culture applications
US6576265B1 (en)	1999-12-22	2003-06-10	Acell, Inc.	Tissue regenerative composition, method of making, and method of use thereof
US6579538B1 (en)	1999-12-22	2003-06-17	Acell, Inc.	Tissue regenerative compositions for cardiac applications, method of making, and method of use thereof
US20040043006A1 (en) *	2002-08-27	2004-03-04	Badylak Stephen F.	Tissue regenerative composition
EP1263285A2 (fr) *	2000-03-14	2002-12-11	Alnis Biosciences, Inc.	Systeme de cryoprotection
EP1269840A4 (fr) *	2000-03-24	2009-05-06	Menicon Co Ltd	Procede de conservation d'equivalent au tissu et equivalent au tissu conserver a l'etat congele
US6521402B1 (en)	2000-06-14	2003-02-18	The Texas A&M University System	Cryopreservation of tissues for use in nuclear transfer
US20020069649A1 (en) *	2000-12-08	2002-06-13	Ardais Corporation	Container for cryopreserved material
DE10061968A1 (de) *	2000-12-13	2002-06-20	Friedrich Hoffmann	Verfahren und Vorrichtung zum Einfrieren von Hornhautgewebe
WO2002047479A2 (fr) *	2000-12-15	2002-06-20	The Texas A & M University System	Clonage de bovins par transplantation nucleaire
AU2002246747A1 (en)	2000-12-27	2002-08-06	Ortec International, Inc.	Processes for making cryopreserved composite living constructs and products resulting therefrom
NZ526793A (en) *	2001-01-02	2006-02-24	Supachill Technologies Pty Ltd	Method and system for preparing tissue samples for histological pathological examination
WO2003024496A1 (fr) *	2001-09-05	2003-03-27	Hans Biomed Corporation	Procede de preparation de biomateriau de reparation tissulaire
WO2003024213A1 (fr) *	2001-09-17	2003-03-27	Provincia Italiana Della Congregazione Dei Figli Dell'immacolata Concezione - Istituto Dermopatico Dell'immacolata	Boitier de protection et de transport de greffons d'epiderme ou de peau cultives in vitro a partir d'un greffon de peau preleve sur un donneur
US6656380B2 (en)	2001-10-16	2003-12-02	Supachill Technologies Pty. Ltd.	Super-coolable composition having long-duration phase change capability, process for preparation of same, process for super-cooling same and articles comprising same
DE10151296A1 (de) *	2001-10-17	2003-04-30	Boehringer Ingelheim Pharma	Keratinozyten verwendbar als biologisch aktive Substanz bei der Behandlung von Wunden
CN1229018C (zh) *	2001-11-01	2005-11-30	综合生物系统公司	用于冷冻、贮存和解冻生物制药材料的系统和方法
US6681581B2 (en)	2001-11-20	2004-01-27	Supachill Technologies Pty. Ltd.	Pre-conditioned solute for use in cryogenic processes
UA46673C2 (en) *	2002-01-14	2005-04-15	Halyna Stepanivna Lobintseva	Method of cryopreservation of human hemopoietic cells
AU2003219830A1 (en) *	2002-02-22	2003-09-09	Ebi, L.P.	Methods and compositions for treating bone or cartilage defects
AU2003268033A1 (en) *	2002-07-26	2004-02-16	The General Hospital Corporation	Systems and methods for cell preservation
WO2004010780A2 (fr) *	2002-07-30	2004-02-05	General Biotechnology, Llc	Glace intracellulaire inoffensive
AU2003303894A1 (en) *	2003-01-30	2004-08-30	The Regents Of The University Of California	Inactivated probiotic bacteria and methods of use thereof
US20040175366A1 (en) *	2003-03-07	2004-09-09	Acell, Inc.	Scaffold for cell growth and differentiation
US20040176855A1 (en) *	2003-03-07	2004-09-09	Acell, Inc.	Decellularized liver for repair of tissue and treatment of organ deficiency
CA2530490A1 (fr)	2003-06-25	2005-01-13	Stephen F. Badylak	Compositions matricielles conditionnees pour la restauration d'un tissu
FR2858057B1 (fr) *	2003-07-21	2006-05-26	Abx Sa	Dispositif de controle de qualite pour analyseur sanguin fonctionnant en sang total
JP2008501320A (ja)	2004-06-02	2008-01-24	イーエス・セル・インターナショナル・プライヴェート・リミテッド	細胞保存方法
RU2268590C1 (ru) *	2004-06-15	2006-01-27	Государственное образовательное учреждение высшего профессионального образования Московская медицинская академия им. И.М. Сеченова	Способ криоконсервации органов и тканей in situ
CN100386061C (zh) *	2005-05-17	2008-05-07	浙江大学医学院附属邵逸夫医院	一种经冰冻处理的低抗原的异种角膜基质及制备方法
JP5478884B2 (ja)	2005-09-26	2014-04-23	ライフセルコーポレーション	乾燥血小板組成物
CN103173401A (zh) *	2005-12-22	2013-06-26	简·恩尼斯	来自冷冻的脐带组织的活细胞
KR101039758B1 (ko)	2006-04-28	2011-06-09	젤티크 애스세틱스, 인코포레이티드.	피하 지질 과다 세포의 개선된 냉각을 위한 치료 장치와함께 사용하기 위한 동결 방지제
WO2008022651A1 (fr)	2006-08-21	2008-02-28	Antoine Turzi	Procédé et dispositif de préparation de plasma riche en plaquettes pour utilisation impromptue et sa combinaison avec des cellules cutanées et osseuses
US9132031B2 (en)	2006-09-26	2015-09-15	Zeltiq Aesthetics, Inc.	Cooling device having a plurality of controllable cooling elements to provide a predetermined cooling profile
US20080077201A1 (en)	2006-09-26	2008-03-27	Juniper Medical, Inc.	Cooling devices with flexible sensors
US8192474B2 (en)	2006-09-26	2012-06-05	Zeltiq Aesthetics, Inc.	Tissue treatment methods
US20080287839A1 (en)	2007-05-18	2008-11-20	Juniper Medical, Inc.	Method of enhanced removal of heat from subcutaneous lipid-rich cells and treatment apparatus having an actuator
US8523927B2 (en)	2007-07-13	2013-09-03	Zeltiq Aesthetics, Inc.	System for treating lipid-rich regions
EP3488833B1 (fr)	2007-08-21	2025-10-22	Zeltiq Aesthetics, Inc.	Surveillance du refroidissement de cellules riches en lipides sous-cutanés, tel que le refroidissement du tissu adipeux
US8603073B2 (en)	2008-12-17	2013-12-10	Zeltiq Aesthetics, Inc.	Systems and methods with interrupt/resume capabilities for treating subcutaneous lipid-rich cells
EP2221362A1 (fr) *	2009-02-19	2010-08-25	Naturin GmbH & Co	Procédé de cryoconservation de cellules, constructions cellulaires artificielles ou ensembles de tissus complexes tridimensionnels
JP5519000B2 (ja)	2009-04-30	2014-06-11	ゼルティックエステティックスインコーポレイテッド	脂肪が豊富な皮下細胞から熱を除去するためのデバイス、システム、及び方法
CA2787374A1 (fr) *	2010-01-25	2011-07-28	Zeltiq Aesthetics, Inc.	Applicateurs a usage domestique pour eliminer de facon non invasive de la chaleur de cellules sous-cutanees riches en lipides par l'intermediaire d'agents de refroidissement a changement de phase, et dispositifs, systemes et procedes associes
CA2790436A1 (fr)	2010-02-18	2011-08-25	Osiris Therapeutics, Inc.	Produits de membrane chorionique immunocompatible
KR101089614B1 (ko) *	2010-02-26	2011-12-05	(주)시지바이오	무세포 진피 기질의 제조 방법 및 그로부터 제조된 무세포 진피 기질
GB201004072D0 (en)	2010-03-11	2010-04-28	Turzi Antoine	Process, tube and device for the preparation of wound healant composition
RU2420736C1 (ru) *	2010-03-15	2011-06-10	Государственное образовательное учреждение высшего профессионального образования "Красноярский государственный медицинский университет имени профессора В.Ф. Войно - Ясенецкого Министерства здравоохранения и социального развития Российской Федерации"	Способ заливки тканей глаза
CN103179852B (zh) *	2010-05-28	2015-04-08	格尼亚有限公司	改良的显微操作和储存装置以及方法
US8676338B2 (en)	2010-07-20	2014-03-18	Zeltiq Aesthetics, Inc.	Combined modality treatment systems, methods and apparatus for body contouring applications
SG188466A1 (en) *	2010-09-13	2013-05-31	Singapore Health Serv Pte Ltd	Method for performing a reversible refractive procedure
JP5915977B2 (ja) *	2011-11-29	2016-05-11	学校法人明治大学	凍結細胞シートの製造方法
WO2013174769A1 (fr)	2012-05-25	2013-11-28	Boehringer Ingelheim International Gmbh	Utilisation de kératinocytes en tant que substance biologiquement active dans le traitement des plaies, par exemple des plaies diabétiques, facultativement en combinaison avec un inhibiteur de la dpp-4
DE102012013267A1 (de)	2012-07-04	2014-01-09	Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.	Substrateinrichtung, Konservierungsgerät und Verfahren zur Kryokonservierung einer biologischen Probe
TW201422155A (zh)	2012-07-06	2014-06-16	Arigos Biomedical Inc	以氣體灌注快速冷卻與升溫預防玻璃質化組織受熱-力破壞的方法與裝置
CA3071928A1 (fr) *	2013-03-13	2014-10-02	Stratatech Corporation	Cryoconservation de substituts de peau humaine viables
US9545523B2 (en)	2013-03-14	2017-01-17	Zeltiq Aesthetics, Inc.	Multi-modality treatment systems, methods and apparatus for altering subcutaneous lipid-rich tissue
US9844460B2 (en)	2013-03-14	2017-12-19	Zeltiq Aesthetics, Inc.	Treatment systems with fluid mixing systems and fluid-cooled applicators and methods of using the same
ES2974899T3 (es)	2014-01-31	2024-07-02	Zeltiq Aesthetics Inc	Composiciones y sistemas de tratamiento para el enfriamiento mejorado de tejido rico en lípidos
US10675176B1 (en)	2014-03-19	2020-06-09	Zeltiq Aesthetics, Inc.	Treatment systems, devices, and methods for cooling targeted tissue
USD777338S1 (en)	2014-03-20	2017-01-24	Zeltiq Aesthetics, Inc.	Cryotherapy applicator for cooling tissue
US10952891B1 (en)	2014-05-13	2021-03-23	Zeltiq Aesthetics, Inc.	Treatment systems with adjustable gap applicators and methods for cooling tissue
US10935174B2 (en)	2014-08-19	2021-03-02	Zeltiq Aesthetics, Inc.	Stress relief couplings for cryotherapy apparatuses
US10568759B2 (en)	2014-08-19	2020-02-25	Zeltiq Aesthetics, Inc.	Treatment systems, small volume applicators, and methods for treating submental tissue
JP6130868B2 (ja) *	2015-02-19	2017-05-17	テルモ株式会社	単離されたシート状細胞培養物の製造方法
US11154418B2 (en)	2015-10-19	2021-10-26	Zeltiq Aesthetics, Inc.	Vascular treatment systems, cooling devices, and methods for cooling vascular structures
AU2017206118B2 (en)	2016-01-07	2021-09-30	Zeltiq Aesthetics, Inc.	Temperature-dependent adhesion between applicator and skin during cooling of tissue
US10765552B2 (en)	2016-02-18	2020-09-08	Zeltiq Aesthetics, Inc.	Cooling cup applicators with contoured heads and liner assemblies
US11382790B2 (en)	2016-05-10	2022-07-12	Zeltiq Aesthetics, Inc.	Skin freezing systems for treating acne and skin conditions
US10555831B2 (en)	2016-05-10	2020-02-11	Zeltiq Aesthetics, Inc.	Hydrogel substances and methods of cryotherapy
US10682297B2 (en)	2016-05-10	2020-06-16	Zeltiq Aesthetics, Inc.	Liposomes, emulsions, and methods for cryotherapy
BR112019006765B1 (pt) *	2016-10-04	2023-09-26	Membrane Protective Technologies, Inc	Método para congelar células e tecidos
EP3573458A4 (fr) *	2017-01-27	2020-12-16	Stratatech Corporation	Systèmes de récipient de tissu
AU2018214954B2 (en) *	2017-02-01	2023-07-13	The United States Of America, As Represented By The Secretary, Department Of Health And Human Services	Devices for tissue cryopreservation and recovery
RU2650694C1 (ru) *	2017-02-15	2018-04-17	Федеральное государственное бюджетное учреждение "Государственный научный центр Российской Федерации - Федеральный медицинский биофизический центр имени А.И. Бурназяна" (ФГБУ ГНЦ ФМБЦ им. А.И. Бурназяна ФМБА России)	Способ эффективной и безопасной криоконсервации донорских сосудистых трансплантатов, обеспечивающий оптимизацию их дальнейшего процессинга - радиационной стерилизации и децеллюляризации
US11076879B2 (en)	2017-04-26	2021-08-03	Zeltiq Aesthetics, Inc.	Shallow surface cryotherapy applicators and related technology
WO2019124339A1 (fr) *	2017-12-18	2019-06-27	テルモ株式会社	Dispositif de retenue d'objet fragile pourvu d'un mécanisme de protection
CN112789013A (zh)	2018-07-31	2021-05-11	斯尔替克美学股份有限公司	改善肤质的方法、装置和系统
CN112638157B (zh) *	2018-08-30	2024-01-09	密苏里大学管理机构	一种防止样品和细胞外冰直接接触的有效低温保存装置
CA3112767C (fr)	2018-09-14	2021-06-15	University Of Miami	Flacon a double chambre pour conservation de greffe corneenne
IL281423B2 (en)	2018-09-20	2024-08-01	Iovance Biotherapeutics Inc	Expansion of TILS from cryopreserved tumor samples
CA3127191A1 (fr)	2019-01-21	2020-07-30	Eclipse Medcorp, Llc	Procede, systeme et appareil pour separer des composants d'un echantillon biologique
US10610280B1 (en)	2019-02-02	2020-04-07	Ayad K. M. Agha	Surgical method and apparatus for destruction and removal of intraperitoneal, visceral, and subcutaneous fat
MX2022004999A (es)	2019-10-31	2022-07-27	Eclipse Medcorp Llc	Sistemas, metodos y aparatos para separar componentes de una muestra.
RU2731287C1 (ru) *	2019-11-21	2020-09-01	Федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский государственный педиатрический медицинский университет" Министерства здравоохранения Российской Федерации (ФГБОУ ВО СПбГПМУ Минздрава России)	Пробирка для криоконсервации ткани яичников
RU2731065C1 (ru) *	2019-12-24	2020-08-28	Федеральное государственное бюджетное учреждение науки Институт биологии развития им. Н.К. Кольцова РАН (ИБР РАН)	Состав криоконсерванта для длительного хранения первичных кератиноцитов
WO2022025240A1 (fr) *	2020-07-31	2022-02-03	学校法人同志社	Procédé et réceptacle pour la conservation de cellules endothéliales cornéennes
WO2023002974A1 (fr) *	2021-07-21	2023-01-26	株式会社北里コーポレーション	Plateau de travail pour le traitement de cellules biologiques et kit de travail pour le traitement de précongélation de cellules biologiques
WO2023196877A1 (fr)	2022-04-06	2023-10-12	Iovance Biotherapeutics, Inc.	Traitement de patients souffrant de cpnpc avec des thérapies lymphocytaires infiltrant les tumeurs
CA3251533A1 (fr)	2022-05-10	2023-11-16	Iovance Biotherapeutics, Inc.	Traitement de patients atteints d'un cancer avec des thérapies lymphocytaires infiltrant les tumeurs en combinaison avec un agoniste d'il-15r

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US2348448A (en) *	1942-02-16	1944-05-09	Kimble Glass Co	Apparatus for the cultivation of anaerobic and microaerophilic organisms
US3842831A (en) *	1972-10-27	1974-10-22	Genetic Labor Inc	Cellular skin patch
FR2486915A1 (fr) *	1980-07-21	1982-01-22	Biomerieux Sa	Boite etanche a fermeture par joint
US4539716A (en) *	1981-03-19	1985-09-10	Massachusetts Institute Of Technology	Fabrication of living blood vessels and glandular tissues
US4546500A (en) *	1981-05-08	1985-10-15	Massachusetts Institute Of Technology	Fabrication of living blood vessels and glandular tissues
US4485096A (en) *	1982-02-26	1984-11-27	Massachusetts Institute Of Technology	Tissue-equivalent and method for preparation thereof
US4485097A (en) *	1982-05-26	1984-11-27	Massachusetts Institute Of Technology	Bone-equivalent and method for preparation thereof
FR2558337B1 (fr) *	1984-01-19	1986-05-02	Air Liquide	Dispositif de congelation de produits biologiques conditionnes en paillettes
US5084377A (en) *	1984-09-19	1992-01-28	Larry Rowan	Cryogenic suspension method
US4604346A (en) *	1984-10-09	1986-08-05	Massachusetts Institute Of Technology	Skin-equivalent prepared by the use of punch biopsy
US5026649A (en) *	1986-03-20	1991-06-25	Costar Corporation	Apparatus for growing tissue cultures in vitro
US4890457A (en) *	1987-01-02	1990-01-02	Cryolife, Inc.	Method for cryopreserving heart valves
IT1207525B (it) *	1987-06-23	1989-05-25	Ist Naz Ric Sul Cancro	Metodo per la preservazione difogli trapiantabili di epitelio coltivato in vitro vitale.
US4978540A (en) *	1988-01-20	1990-12-18	Lee Tung Ching	Production of frozen foods and other products
US5194269A (en) *	1988-01-20	1993-03-16	Lee Tung Ching	Production of frozen foods and other products
US4837379A (en) *	1988-06-02	1989-06-06	Organogenesis Inc.	Fibrin-collagen tissue equivalents and methods for preparation thereof
CA1335723C (fr) *	1988-08-04	1995-05-30	Thomas Glonek	Methode et composition pour la cryoconservation de tissue
CA2000181A1 (fr) *	1988-10-14	1990-04-14	Chung-Faye Chao	Methode de culture de lambeaux d'epiderme, produit et methode
JPH0651601B2 (ja) *	1988-10-27	1994-07-06	伊藤忠飼料株式会社	豚の凍結受精卵及び凍結保存方法
JPH02300101A (ja) *	1989-05-12	1990-12-12	Tabai Espec Corp	生物試料凍結保存装置における植氷方法および植氷装置
US4988302A (en) *	1989-06-08	1991-01-29	Difco Laboratories Incorporated	Culture media package
US5131850A (en) *	1989-11-03	1992-07-21	Cryolife, Inc.	Method for cryopreserving musculoskeletal tissues
EP0502033A1 (fr) *	1989-11-20	1992-09-09	Cell Systems Ltd	Procede et appareil de refroidissement
US5040677A (en) *	1990-06-04	1991-08-20	Biosurface Technology, Inc.	Container for storage and distribution of a skin wound dressing
US5145770A (en) *	1990-06-04	1992-09-08	Biosurface Technology, Inc.	Cryopreservation of cultured epithelial sheets
US5336616A (en) *	1990-09-12	1994-08-09	Lifecell Corporation	Method for processing and preserving collagen-based tissues for transplantation
JPH04365434A (ja) *	1991-06-12	1992-12-17	Oji Paper Co Ltd	不定根および／または毛状根の凍結保存法
JP3672201B2 (ja) *	1991-12-26	2005-07-20	雪印乳業株式会社	哺乳動物胚移植用ストロー及びその製造法
US5366893A (en) *	1993-01-13	1994-11-22	Becton, Dickinson And Company	Culture vessel
US5518878A (en) *	1993-09-15	1996-05-21	Organogenesis Inc.	Cryopreservation of cultured skin or cornea equivalents with agitation
US5891617A (en) *	1993-09-15	1999-04-06	Organogenesis Inc.	Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing
US5689961A (en) *	1996-01-30	1997-11-25	Organogenesis Inc.	Ice seeding apparatus for cryopreservation systems

1995
- 1995-01-30 US US08/380,099 patent/US5891617A/en not_active Expired - Lifetime
1996
- 1996-01-30 HU HU9800411A patent/HU223789B1/hu not_active IP Right Cessation
- 1996-01-30 ES ES96905275T patent/ES2231804T3/es not_active Expired - Lifetime
- 1996-01-30 EP EP96905275A patent/EP0807234B1/fr not_active Expired - Lifetime
- 1996-01-30 SK SK1033-97A patent/SK284801B6/sk unknown
- 1996-01-30 RU RU97116150/13A patent/RU2178865C2/ru not_active IP Right Cessation
- 1996-01-30 WO PCT/US1996/001217 patent/WO1996024018A1/fr not_active Ceased
- 1996-01-30 SG SG9802806A patent/SG106553A1/en unknown
- 1996-01-30 AU AU49082/96A patent/AU716869B2/en not_active Ceased
- 1996-01-30 DE DE69633854T patent/DE69633854T2/de not_active Expired - Lifetime
- 1996-01-30 PT PT96905275T patent/PT807234E/pt unknown
- 1996-01-30 AT AT04077072T patent/ATE376356T1/de not_active IP Right Cessation
- 1996-01-30 JP JP8523665A patent/JPH11501298A/ja not_active Withdrawn
- 1996-01-30 EP EP04077072A patent/EP1516532B1/fr not_active Expired - Lifetime
- 1996-01-30 PL PL96321089A patent/PL181762B1/pl not_active IP Right Cessation
- 1996-01-30 AT AT96905275T patent/ATE282805T1/de not_active IP Right Cessation
- 1996-01-30 NZ NZ302913A patent/NZ302913A/en unknown
- 1996-01-30 CZ CZ19972413A patent/CZ294950B6/cs not_active IP Right Cessation
- 1996-01-30 KR KR1019970705324A patent/KR100328742B1/ko not_active Expired - Fee Related
- 1996-01-30 CN CNB031457983A patent/CN1292651C/zh not_active Expired - Fee Related
- 1996-01-30 BR BR9606864A patent/BR9606864A/pt not_active IP Right Cessation
- 1996-01-30 CN CN96191673A patent/CN1119599C/zh not_active Expired - Fee Related
- 1996-01-30 DE DE69637301T patent/DE69637301T2/de not_active Expired - Lifetime
1997
- 1997-06-16 FI FI972563A patent/FI113694B/fi active
- 1997-07-28 NO NO19973463A patent/NO310491B1/no unknown
2006
- 2006-07-10 JP JP2006188993A patent/JP4361550B2/ja not_active Expired - Fee Related
2007
- 2007-01-04 JP JP2007000107A patent/JP2007161723A/ja not_active Withdrawn
2013
- 2013-12-27 JP JP2013272171A patent/JP2014065736A/ja active Pending

Also Published As

Publication number	Publication date
DE69637301T2 (de)	2008-08-14
CZ294950B6 (cs)	2005-04-13
AU4908296A (en)	1996-08-21
KR19980701927A (ko)	1998-06-25
DE69633854D1 (de)	2004-12-23
DE69637301D1 (de)	2007-12-06
CN1292651C (zh)	2007-01-03
JP2006325598A (ja)	2006-12-07
EP0807234B1 (fr)	2004-11-17
WO1996024018A1 (fr)	1996-08-08
JP2007161723A (ja)	2007-06-28
SK284801B6 (sk)	2005-11-03
EP0807234A4 (fr)	2002-08-14
HUP9800411A2 (en)	1998-06-29
PL181762B1 (en)	2001-09-28
ES2231804T3 (es)	2005-05-16
PT807234E (pt)	2005-03-31
NO973463L (no)	1997-09-30
JP2014065736A (ja)	2014-04-17
SK103397A3 (en)	1998-06-03
CN1173220A (zh)	1998-02-11
FI113694B (fi)	2004-05-31
KR100328742B1 (ko)	2002-09-17
FI972563A0 (fi)	1997-06-16
CZ241397A3 (en)	1997-11-12
EP1516532B1 (fr)	2007-10-24
PL321089A1 (en)	1997-11-24
RU2178865C2 (ru)	2002-01-27
SG106553A1 (en)	2004-10-29
ATE376356T1 (de)	2007-11-15
JP4361550B2 (ja)	2009-11-11
EP1516532A1 (fr)	2005-03-23
DE69633854T2 (de)	2005-10-20
FI972563L (fi)	1997-09-29
JPH11501298A (ja)	1999-02-02
NO973463D0 (no)	1997-07-28
CN1589622A (zh)	2005-03-09
BR9606864A (pt)	1997-12-23
US5891617A (en)	1999-04-06
NZ302913A (en)	1999-04-29
HU223789B1 (hu)	2005-01-28
ATE282805T1 (de)	2004-12-15
CN1119599C (zh)	2003-08-27
EP0807234A1 (fr)	1997-11-19
AU716869B2 (en)	2000-03-09

Publication	Publication Date	Title
NO310491B1 (no)	2001-07-16	Metode samt apparat for kryokonservering
US5964096A (en)	1999-10-12	Method and package design for cryopreservation and storage of cultured tissue equivalents
AU2021261937B2 (en)	2023-11-16	Cryopreservation of viable human skin substitutes
EP0719084B1 (fr)	2001-12-12	Cryoconservation d'equivalents tissulaires cultives
CA2210532C (fr)	2007-05-15	Procede et structure d'emballage pour la conservation et le stockage cryogeniques d'equivalents de tissu de culture