SK284801B6 - Spôsob kryokonzervácie tkanív cicavcov - Google Patents
Spôsob kryokonzervácie tkanív cicavcov Download PDFInfo
- Publication number
- SK284801B6 SK284801B6 SK1033-97A SK103397A SK284801B6 SK 284801 B6 SK284801 B6 SK 284801B6 SK 103397 A SK103397 A SK 103397A SK 284801 B6 SK284801 B6 SK 284801B6
- Authority
- SK
- Slovakia
- Prior art keywords
- tissue
- temperature
- equivalent
- cryopreserved
- skin
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 64
- 238000005138 cryopreservation Methods 0.000 claims abstract description 41
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 29
- 238000007710 freezing Methods 0.000 claims abstract description 23
- 230000008014 freezing Effects 0.000 claims abstract description 23
- 230000035515 penetration Effects 0.000 claims abstract description 8
- 239000003755 preservative agent Substances 0.000 claims description 59
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 50
- 230000002335 preservative effect Effects 0.000 claims description 50
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 38
- 230000002500 effect on skin Effects 0.000 claims description 29
- 230000010412 perfusion Effects 0.000 claims description 21
- 238000001816 cooling Methods 0.000 claims description 20
- 238000007654 immersion Methods 0.000 claims description 3
- 238000003860 storage Methods 0.000 abstract description 19
- 238000000338 in vitro Methods 0.000 abstract description 16
- 238000002054 transplantation Methods 0.000 abstract description 16
- 210000004087 cornea Anatomy 0.000 abstract description 7
- 238000010899 nucleation Methods 0.000 abstract description 7
- 238000001727 in vivo Methods 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 5
- 239000002577 cryoprotective agent Substances 0.000 abstract description 4
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 238000002513 implantation Methods 0.000 abstract description 2
- 238000010874 in vitro model Methods 0.000 abstract description 2
- 238000012216 screening Methods 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 122
- 210000003491 skin Anatomy 0.000 description 98
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 73
- 210000004027 cell Anatomy 0.000 description 56
- 239000010410 layer Substances 0.000 description 44
- 239000002609 medium Substances 0.000 description 34
- 210000002615 epidermis Anatomy 0.000 description 31
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 30
- 239000000243 solution Substances 0.000 description 30
- 241000699666 Mus <mouse, genus> Species 0.000 description 26
- 239000000523 sample Substances 0.000 description 25
- 238000012360 testing method Methods 0.000 description 23
- 239000007788 liquid Substances 0.000 description 19
- 235000011187 glycerol Nutrition 0.000 description 16
- 238000010257 thawing Methods 0.000 description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 14
- 239000001569 carbon dioxide Substances 0.000 description 14
- 229910002092 carbon dioxide Inorganic materials 0.000 description 14
- 239000013078 crystal Substances 0.000 description 12
- 239000012595 freezing medium Substances 0.000 description 12
- 241000699670 Mus sp. Species 0.000 description 10
- 230000035899 viability Effects 0.000 description 9
- 230000006870 function Effects 0.000 description 8
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 102000008186 Collagen Human genes 0.000 description 6
- 108010035532 Collagen Proteins 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 229920001436 collagen Polymers 0.000 description 6
- 230000018044 dehydration Effects 0.000 description 6
- 238000006297 dehydration reaction Methods 0.000 description 6
- 210000002950 fibroblast Anatomy 0.000 description 6
- 239000007789 gas Substances 0.000 description 6
- 230000003834 intracellular effect Effects 0.000 description 6
- 230000006911 nucleation Effects 0.000 description 6
- 239000002356 single layer Substances 0.000 description 6
- 208000027418 Wounds and injury Diseases 0.000 description 5
- 230000006378 damage Effects 0.000 description 5
- 210000002919 epithelial cell Anatomy 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000002829 reductive effect Effects 0.000 description 5
- 238000009827 uniform distribution Methods 0.000 description 5
- 238000004017 vitrification Methods 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 239000000969 carrier Substances 0.000 description 4
- 230000005779 cell damage Effects 0.000 description 4
- 208000037887 cell injury Diseases 0.000 description 4
- 230000003833 cell viability Effects 0.000 description 4
- 210000001339 epidermal cell Anatomy 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000007791 liquid phase Substances 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 230000002503 metabolic effect Effects 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 229940105631 nembutal Drugs 0.000 description 4
- 210000000056 organ Anatomy 0.000 description 4
- WEXRUCMBJFQVBZ-UHFFFAOYSA-N pentobarbital Chemical compound CCCC(C)C1(CC)C(=O)NC(=O)NC1=O WEXRUCMBJFQVBZ-UHFFFAOYSA-N 0.000 description 4
- 230000035699 permeability Effects 0.000 description 4
- 239000012071 phase Substances 0.000 description 4
- 230000001681 protective effect Effects 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 101710088194 Dehydrogenase Proteins 0.000 description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 3
- 231100000002 MTT assay Toxicity 0.000 description 3
- 238000000134 MTT assay Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 239000012620 biological material Substances 0.000 description 3
- 238000001574 biopsy Methods 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 239000006143 cell culture medium Substances 0.000 description 3
- 210000004207 dermis Anatomy 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000012894 fetal calf serum Substances 0.000 description 3
- 210000002510 keratinocyte Anatomy 0.000 description 3
- 239000008101 lactose Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- NBWRJAOOMGASJP-UHFFFAOYSA-N 2-(3,5-diphenyl-1h-tetrazol-1-ium-2-yl)-4,5-dimethyl-1,3-thiazole;bromide Chemical compound [Br-].S1C(C)=C(C)N=C1N1N(C=2C=CC=CC=2)N=C(C=2C=CC=CC=2)[NH2+]1 NBWRJAOOMGASJP-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 208000025865 Ulcer Diseases 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 235000011089 carbon dioxide Nutrition 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000000512 collagen gel Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 238000002425 crystallisation Methods 0.000 description 2
- 230000008025 crystallization Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 230000006910 ice nucleation Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 239000002085 irritant Substances 0.000 description 2
- 231100000021 irritant Toxicity 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000001000 micrograph Methods 0.000 description 2
- 230000002438 mitochondrial effect Effects 0.000 description 2
- 230000000394 mitotic effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000004781 supercooling Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 230000010388 wound contraction Effects 0.000 description 2
- 241000282465 Canis Species 0.000 description 1
- 102000004266 Collagen Type IV Human genes 0.000 description 1
- 108010042086 Collagen Type IV Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 206010056340 Diabetic ulcer Diseases 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 208000000558 Varicose Ulcer Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 238000005280 amorphization Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000007630 basic procedure Methods 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000003570 cell viability assay Methods 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 210000000399 corneal endothelial cell Anatomy 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 210000000871 endothelium corneal Anatomy 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000005175 epidermal keratinocyte Anatomy 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000009477 glass transition Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000004898 mitochondrial function Effects 0.000 description 1
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000011580 nude mouse model Methods 0.000 description 1
- 125000000962 organic group Chemical group 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 1
- 230000008458 response to injury Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 210000005122 simple epithelium Anatomy 0.000 description 1
- 238000007390 skin biopsy Methods 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- -1 sodium NADH salts Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 210000002536 stromal cell Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- 231100000816 toxic dose Toxicity 0.000 description 1
- 231100000721 toxic potential Toxicity 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 230000036269 ulceration Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/14—Mechanical aspects of preservation; Apparatus or containers therefor
- A01N1/146—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/16—Physical preservation processes
- A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D55/00—Accessories for container closures not otherwise provided for
- B65D55/02—Locking devices; Means for discouraging or indicating unauthorised opening or removal of closure
- B65D55/06—Deformable or tearable wires, strings or strips; Use of seals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D77/00—Packages formed by enclosing articles or materials in preformed containers, e.g. boxes, cartons, sacks or bags
- B65D77/04—Articles or materials enclosed in two or more containers disposed one within another
- B65D77/048—Articles or materials enclosed in two or more containers disposed one within another the inner and outer containers being rigid and the outer container being of curved cross-section, e.g. cylindrical
- B65D77/0486—Articles or materials enclosed in two or more containers disposed one within another the inner and outer containers being rigid and the outer container being of curved cross-section, e.g. cylindrical the inner container being coaxially disposed within the outer container
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D85/00—Containers, packaging elements or packages, specially adapted for particular articles or materials
- B65D85/50—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Mechanical Engineering (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Packging For Living Organisms, Food Or Medicinal Products That Are Sensitive To Environmental Conditiond (AREA)
- Packages (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Catching Or Destruction (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/380,099 US5891617A (en) | 1993-09-15 | 1995-01-30 | Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing |
| PCT/US1996/001217 WO1996024018A1 (fr) | 1995-01-30 | 1996-01-30 | Procede et structure d'emballage pour la conservation et le stockage cryogeniques d'equivalents de tissu de culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SK103397A3 SK103397A3 (en) | 1998-06-03 |
| SK284801B6 true SK284801B6 (sk) | 2005-11-03 |
Family
ID=23499896
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SK1033-97A SK284801B6 (sk) | 1995-01-30 | 1996-01-30 | Spôsob kryokonzervácie tkanív cicavcov |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US5891617A (fr) |
| EP (2) | EP1516532B1 (fr) |
| JP (4) | JPH11501298A (fr) |
| KR (1) | KR100328742B1 (fr) |
| CN (2) | CN1292651C (fr) |
| AT (2) | ATE376356T1 (fr) |
| AU (1) | AU716869B2 (fr) |
| BR (1) | BR9606864A (fr) |
| CZ (1) | CZ294950B6 (fr) |
| DE (2) | DE69637301T2 (fr) |
| ES (1) | ES2231804T3 (fr) |
| FI (1) | FI113694B (fr) |
| HU (1) | HU223789B1 (fr) |
| NO (1) | NO310491B1 (fr) |
| NZ (1) | NZ302913A (fr) |
| PL (1) | PL181762B1 (fr) |
| PT (1) | PT807234E (fr) |
| RU (1) | RU2178865C2 (fr) |
| SG (1) | SG106553A1 (fr) |
| SK (1) | SK284801B6 (fr) |
| WO (1) | WO1996024018A1 (fr) |
Families Citing this family (96)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5891617A (en) * | 1993-09-15 | 1999-04-06 | Organogenesis Inc. | Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing |
| US5689961A (en) | 1996-01-30 | 1997-11-25 | Organogenesis Inc. | Ice seeding apparatus for cryopreservation systems |
| GB2330516A (en) * | 1997-10-22 | 1999-04-28 | Elizabeth Acton | Cryopreservation of cell suspensions |
| US6291240B1 (en) * | 1998-01-29 | 2001-09-18 | Advanced Tissue Sciences, Inc. | Cells or tissues with increased protein factors and methods of making and using same |
| DE69929691T2 (de) | 1998-05-26 | 2006-09-07 | Lifecell Corp. | Cryokonservierung menschlicher roter blutzellen |
| WO2000029553A1 (fr) | 1998-11-19 | 2000-05-25 | Organogenesis Inc. | Constructions tissulaires transgeniques et procedes de production et d'utilisation associes |
| US20010036665A1 (en) * | 1999-03-18 | 2001-11-01 | Susan M. Young | Method of preparing cryogenically preserved adherent cell containing plate for tissue culture applications |
| US20040043006A1 (en) * | 2002-08-27 | 2004-03-04 | Badylak Stephen F. | Tissue regenerative composition |
| US6576265B1 (en) * | 1999-12-22 | 2003-06-10 | Acell, Inc. | Tissue regenerative composition, method of making, and method of use thereof |
| US6579538B1 (en) | 1999-12-22 | 2003-06-17 | Acell, Inc. | Tissue regenerative compositions for cardiac applications, method of making, and method of use thereof |
| AU2001252914A1 (en) * | 2000-03-14 | 2001-09-24 | Alnis Bioscience, Inc. | Cryoprotective system |
| EP1269840A4 (fr) * | 2000-03-24 | 2009-05-06 | Menicon Co Ltd | Procede de conservation d'equivalent au tissu et equivalent au tissu conserver a l'etat congele |
| US6521402B1 (en) | 2000-06-14 | 2003-02-18 | The Texas A&M University System | Cryopreservation of tissues for use in nuclear transfer |
| AU2002227255A1 (en) * | 2000-12-08 | 2002-06-18 | Ardais Corporation | Container for cryopreserved material |
| DE10061968A1 (de) * | 2000-12-13 | 2002-06-20 | Friedrich Hoffmann | Verfahren und Vorrichtung zum Einfrieren von Hornhautgewebe |
| AU2002226094A1 (en) * | 2000-12-15 | 2002-06-24 | The Texas A And M University System | Cloning bovines by nuclear transplantation |
| IL157532A0 (en) | 2000-12-27 | 2004-03-28 | Ortec International Inc | Processes for making cryopreserved composite living constructs and products resulting therefrom |
| RU2003124058A (ru) * | 2001-01-02 | 2005-01-10 | Сьюпачилл Текнолоджиз Пти. Лтд. (Au) | Способ и система приготовления образцов тканей для гистологического и патологического исследования |
| KR100514582B1 (ko) * | 2001-09-05 | 2005-09-13 | 한스바이오메드 주식회사 | 생체복원물질의 제조방법 |
| WO2003024213A1 (fr) * | 2001-09-17 | 2003-03-27 | Provincia Italiana Della Congregazione Dei Figli Dell'immacolata Concezione - Istituto Dermopatico Dell'immacolata | Boitier de protection et de transport de greffons d'epiderme ou de peau cultives in vitro a partir d'un greffon de peau preleve sur un donneur |
| US6656380B2 (en) | 2001-10-16 | 2003-12-02 | Supachill Technologies Pty. Ltd. | Super-coolable composition having long-duration phase change capability, process for preparation of same, process for super-cooling same and articles comprising same |
| DE10151296A1 (de) * | 2001-10-17 | 2003-04-30 | Boehringer Ingelheim Pharma | Keratinozyten verwendbar als biologisch aktive Substanz bei der Behandlung von Wunden |
| CA2466130A1 (fr) * | 2001-11-01 | 2003-05-08 | Nicolas Voute | Systemes et procedes pour congeler et stocker des materiaux biopharmaceutiques |
| US6681581B2 (en) | 2001-11-20 | 2004-01-27 | Supachill Technologies Pty. Ltd. | Pre-conditioned solute for use in cryogenic processes |
| UA46673C2 (en) * | 2002-01-14 | 2005-04-15 | Halyna Stepanivna Lobintseva | Method of cryopreservation of human hemopoietic cells |
| AU2003219830A1 (en) * | 2002-02-22 | 2003-09-09 | Ebi, L.P. | Methods and compositions for treating bone or cartilage defects |
| AU2003268033A1 (en) * | 2002-07-26 | 2004-02-16 | The General Hospital Corporation | Systems and methods for cell preservation |
| WO2004010780A2 (fr) * | 2002-07-30 | 2004-02-05 | General Biotechnology, Llc | Glace intracellulaire inoffensive |
| WO2004069156A2 (fr) * | 2003-01-30 | 2004-08-19 | The Regents Of The University Of California | Bacteries probiotiques inactivees et leurs procedes d'utilisation |
| US20040176855A1 (en) * | 2003-03-07 | 2004-09-09 | Acell, Inc. | Decellularized liver for repair of tissue and treatment of organ deficiency |
| US20040175366A1 (en) * | 2003-03-07 | 2004-09-09 | Acell, Inc. | Scaffold for cell growth and differentiation |
| US20050025838A1 (en) * | 2003-06-25 | 2005-02-03 | Badylak Stephen F. | Conditioned compositions for tissue restoration |
| FR2858057B1 (fr) * | 2003-07-21 | 2006-05-26 | Abx Sa | Dispositif de controle de qualite pour analyseur sanguin fonctionnant en sang total |
| JP2008501320A (ja) | 2004-06-02 | 2008-01-24 | イーエス・セル・インターナショナル・プライヴェート・リミテッド | 細胞保存方法 |
| RU2268590C1 (ru) * | 2004-06-15 | 2006-01-27 | Государственное образовательное учреждение высшего профессионального образования Московская медицинская академия им. И.М. Сеченова | Способ криоконсервации органов и тканей in situ |
| CN100386061C (zh) * | 2005-05-17 | 2008-05-07 | 浙江大学医学院附属邵逸夫医院 | 一种经冰冻处理的低抗原的异种角膜基质及制备方法 |
| US20090035289A1 (en) | 2005-09-26 | 2009-02-05 | Lifecell Corporation | Dry platelet composition |
| EP1976980B1 (fr) * | 2005-12-22 | 2019-03-06 | Jane Ennis | Cellules viables obtenues de tissu de cordon ombilical congele |
| BRPI0706055B8 (pt) | 2006-04-28 | 2021-06-22 | Zeltiq Aesthetics Inc | sistema e conjunto de componentes para remover calor de células subcutâneas ricas em lipídios |
| WO2008022651A1 (fr) | 2006-08-21 | 2008-02-28 | Antoine Turzi | Procédé et dispositif de préparation de plasma riche en plaquettes pour utilisation impromptue et sa combinaison avec des cellules cutanées et osseuses |
| US9132031B2 (en) | 2006-09-26 | 2015-09-15 | Zeltiq Aesthetics, Inc. | Cooling device having a plurality of controllable cooling elements to provide a predetermined cooling profile |
| US8192474B2 (en) | 2006-09-26 | 2012-06-05 | Zeltiq Aesthetics, Inc. | Tissue treatment methods |
| US20080077201A1 (en) * | 2006-09-26 | 2008-03-27 | Juniper Medical, Inc. | Cooling devices with flexible sensors |
| US20080287839A1 (en) | 2007-05-18 | 2008-11-20 | Juniper Medical, Inc. | Method of enhanced removal of heat from subcutaneous lipid-rich cells and treatment apparatus having an actuator |
| US8523927B2 (en) | 2007-07-13 | 2013-09-03 | Zeltiq Aesthetics, Inc. | System for treating lipid-rich regions |
| WO2009026471A1 (fr) | 2007-08-21 | 2009-02-26 | Zeltiq Aesthetics, Inc. | Surveillance du refroidissement de cellules riches en lipides sous-cutanés, tel que le refroidissement du tissu adipeux |
| US8603073B2 (en) | 2008-12-17 | 2013-12-10 | Zeltiq Aesthetics, Inc. | Systems and methods with interrupt/resume capabilities for treating subcutaneous lipid-rich cells |
| EP2221362A1 (fr) * | 2009-02-19 | 2010-08-25 | Naturin GmbH & Co | Procédé de cryoconservation de cellules, constructions cellulaires artificielles ou ensembles de tissus complexes tridimensionnels |
| SG10201401922TA (en) | 2009-04-30 | 2014-06-27 | Zeltiq Aesthetics Inc | Device, system and method of removing heat from subcutaneous lipid-rich cells |
| EP2528560A1 (fr) | 2010-01-25 | 2012-12-05 | Zeltiq Aesthetics, Inc. | Applicateurs à usage domestique pour éliminer de façon non invasive de la chaleur de cellules sous-cutanées riches en lipides par l'intermédiaire d'agents de refroidissement à changement de phase, et dispositifs, systèmes et procédés associés |
| AR080219A1 (es) | 2010-02-18 | 2012-03-21 | Osiris Therapeutics Inc | Productos de membranas corionicas inmunocompatibles |
| KR101089614B1 (ko) * | 2010-02-26 | 2011-12-05 | (주)시지바이오 | 무세포 진피 기질의 제조 방법 및 그로부터 제조된 무세포 진피 기질 |
| GB201004072D0 (en) | 2010-03-11 | 2010-04-28 | Turzi Antoine | Process, tube and device for the preparation of wound healant composition |
| RU2420736C1 (ru) * | 2010-03-15 | 2011-06-10 | Государственное образовательное учреждение высшего профессионального образования "Красноярский государственный медицинский университет имени профессора В.Ф. Войно - Ясенецкого Министерства здравоохранения и социального развития Российской Федерации" | Способ заливки тканей глаза |
| HUE062745T2 (hu) * | 2010-05-28 | 2023-12-28 | Genea Ip Holdings Pty Ltd | Továbbfejlesztett mikromanipulációs és tároló készülék és eljárások |
| US8676338B2 (en) | 2010-07-20 | 2014-03-18 | Zeltiq Aesthetics, Inc. | Combined modality treatment systems, methods and apparatus for body contouring applications |
| SG188466A1 (en) * | 2010-09-13 | 2013-05-31 | Singapore Health Serv Pte Ltd | Method for performing a reversible refractive procedure |
| JP5915977B2 (ja) * | 2011-11-29 | 2016-05-11 | 学校法人明治大学 | 凍結細胞シートの製造方法 |
| WO2013174769A1 (fr) | 2012-05-25 | 2013-11-28 | Boehringer Ingelheim International Gmbh | Utilisation de kératinocytes en tant que substance biologiquement active dans le traitement des plaies, par exemple des plaies diabétiques, facultativement en combinaison avec un inhibiteur de la dpp-4 |
| DE102012013267A1 (de) * | 2012-07-04 | 2014-01-09 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Substrateinrichtung, Konservierungsgerät und Verfahren zur Kryokonservierung einer biologischen Probe |
| TW201422155A (zh) | 2012-07-06 | 2014-06-16 | Arigos Biomedical Inc | 以氣體灌注快速冷卻與升溫預防玻璃質化組織受熱-力破壞的方法與裝置 |
| ES2705043T3 (es) * | 2013-03-13 | 2019-03-21 | Stratatech Corp | Criopreservación de sustitutos de piel humana viables |
| US9545523B2 (en) | 2013-03-14 | 2017-01-17 | Zeltiq Aesthetics, Inc. | Multi-modality treatment systems, methods and apparatus for altering subcutaneous lipid-rich tissue |
| US9844460B2 (en) | 2013-03-14 | 2017-12-19 | Zeltiq Aesthetics, Inc. | Treatment systems with fluid mixing systems and fluid-cooled applicators and methods of using the same |
| EP3099261A2 (fr) | 2014-01-31 | 2016-12-07 | Zeltiq Aesthetics, Inc. | Systèmes de traitement destinés au traitement de la cellulite par refroidissement |
| US10675176B1 (en) | 2014-03-19 | 2020-06-09 | Zeltiq Aesthetics, Inc. | Treatment systems, devices, and methods for cooling targeted tissue |
| USD777338S1 (en) | 2014-03-20 | 2017-01-24 | Zeltiq Aesthetics, Inc. | Cryotherapy applicator for cooling tissue |
| US10952891B1 (en) | 2014-05-13 | 2021-03-23 | Zeltiq Aesthetics, Inc. | Treatment systems with adjustable gap applicators and methods for cooling tissue |
| US10935174B2 (en) | 2014-08-19 | 2021-03-02 | Zeltiq Aesthetics, Inc. | Stress relief couplings for cryotherapy apparatuses |
| US10568759B2 (en) | 2014-08-19 | 2020-02-25 | Zeltiq Aesthetics, Inc. | Treatment systems, small volume applicators, and methods for treating submental tissue |
| JP6130868B2 (ja) * | 2015-02-19 | 2017-05-17 | テルモ株式会社 | 単離されたシート状細胞培養物の製造方法 |
| WO2017070112A1 (fr) | 2015-10-19 | 2017-04-27 | Zeltiq Aesthetics, Inc. | Systèmes de traitement vasculaire, dispositifs de refroidissement et procédés pour refroidir des structures vasculaires |
| CN108472151B (zh) | 2016-01-07 | 2020-10-27 | 斯尔替克美学股份有限公司 | 在组织冷却期间施用器与皮肤之间的温度依赖性粘附 |
| US10765552B2 (en) | 2016-02-18 | 2020-09-08 | Zeltiq Aesthetics, Inc. | Cooling cup applicators with contoured heads and liner assemblies |
| US11382790B2 (en) | 2016-05-10 | 2022-07-12 | Zeltiq Aesthetics, Inc. | Skin freezing systems for treating acne and skin conditions |
| US10682297B2 (en) | 2016-05-10 | 2020-06-16 | Zeltiq Aesthetics, Inc. | Liposomes, emulsions, and methods for cryotherapy |
| US10555831B2 (en) | 2016-05-10 | 2020-02-11 | Zeltiq Aesthetics, Inc. | Hydrogel substances and methods of cryotherapy |
| BR112019006765B1 (pt) * | 2016-10-04 | 2023-09-26 | Membrane Protective Technologies, Inc | Método para congelar células e tecidos |
| CA3049781A1 (fr) * | 2017-01-27 | 2018-08-02 | Stratatech Corporation | Systemes de contenants pour soutenir le transport, la decongelation et l'utilisation de substituts de peau humaine cryoconserves |
| EP3576527B1 (fr) | 2017-02-01 | 2026-05-20 | Us Health | Dispositifs de cryoconservation et de récupération de tissu |
| RU2650694C1 (ru) * | 2017-02-15 | 2018-04-17 | Федеральное государственное бюджетное учреждение "Государственный научный центр Российской Федерации - Федеральный медицинский биофизический центр имени А.И. Бурназяна" (ФГБУ ГНЦ ФМБЦ им. А.И. Бурназяна ФМБА России) | Способ эффективной и безопасной криоконсервации донорских сосудистых трансплантатов, обеспечивающий оптимизацию их дальнейшего процессинга - радиационной стерилизации и децеллюляризации |
| US11076879B2 (en) | 2017-04-26 | 2021-08-03 | Zeltiq Aesthetics, Inc. | Shallow surface cryotherapy applicators and related technology |
| EP3722411B1 (fr) * | 2017-12-18 | 2024-10-16 | Terumo Kabushiki Kaisha | Dispositif de retenue d'objet fragile pourvu d'un mécanisme de protection |
| CA3107932A1 (fr) | 2018-07-31 | 2020-02-06 | Zeltiq Aesthetics, Inc. | Procedes, dispositifs et systemes pour l'amelioration d'une ou de plusieurs caracteristiques de la peau |
| US12408661B2 (en) | 2018-08-30 | 2025-09-09 | The Curators Of The University Of Missouri | Efficient cryopreservation device preventing the direct contact between samples and extracellular ice |
| WO2020056392A1 (fr) | 2018-09-14 | 2020-03-19 | University Of Miami | Flacon à double chambre pour conservation de greffe cornéenne |
| EP3852524B1 (fr) | 2018-09-20 | 2023-06-28 | Iovance Biotherapeutics, Inc. | Expansion de til à partir d'échantillons de tumeur cryoconservés |
| WO2020154305A1 (fr) | 2019-01-21 | 2020-07-30 | Eclipse Medcorp, Llc | Procédé, système et appareil pour séparer des composants d'un échantillon biologique |
| US10610280B1 (en) | 2019-02-02 | 2020-04-07 | Ayad K. M. Agha | Surgical method and apparatus for destruction and removal of intraperitoneal, visceral, and subcutaneous fat |
| US12007382B2 (en) | 2019-10-31 | 2024-06-11 | Crown Laboratories, Inc. | Systems, methods and apparatus for separating components of a sample |
| RU2731287C1 (ru) * | 2019-11-21 | 2020-09-01 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский государственный педиатрический медицинский университет" Министерства здравоохранения Российской Федерации (ФГБОУ ВО СПбГПМУ Минздрава России) | Пробирка для криоконсервации ткани яичников |
| RU2731065C1 (ru) * | 2019-12-24 | 2020-08-28 | Федеральное государственное бюджетное учреждение науки Институт биологии развития им. Н.К. Кольцова РАН (ИБР РАН) | Состав криоконсерванта для длительного хранения первичных кератиноцитов |
| WO2022025240A1 (fr) * | 2020-07-31 | 2022-02-03 | 学校法人同志社 | Procédé et réceptacle pour la conservation de cellules endothéliales cornéennes |
| EP4375358B1 (fr) * | 2021-07-21 | 2025-12-31 | Kitazato Corporation | Plateau de travail pour le traitement de cellules biologiques et kit de travail pour le traitement de précongélation de cellules biologiques |
| CA3247638A1 (fr) | 2022-04-06 | 2023-10-12 | Maria Fardis | Traitement de patients souffrant de carcinome pulmonaire non à petites cellules (cpnpc) avec des thérapies lymphocytaires infiltrant les tumeurs |
| US20250281537A1 (en) | 2022-05-10 | 2025-09-11 | Iovance Biotherapeutics, Inc. | Treatment of cancer patients with tumor infiltrating lymphocyte therapies in combination with an il-15r agonist |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2348448A (en) * | 1942-02-16 | 1944-05-09 | Kimble Glass Co | Apparatus for the cultivation of anaerobic and microaerophilic organisms |
| US3842831A (en) * | 1972-10-27 | 1974-10-22 | Genetic Labor Inc | Cellular skin patch |
| FR2486915A1 (fr) * | 1980-07-21 | 1982-01-22 | Biomerieux Sa | Boite etanche a fermeture par joint |
| US4539716A (en) * | 1981-03-19 | 1985-09-10 | Massachusetts Institute Of Technology | Fabrication of living blood vessels and glandular tissues |
| US4546500A (en) * | 1981-05-08 | 1985-10-15 | Massachusetts Institute Of Technology | Fabrication of living blood vessels and glandular tissues |
| US4485096A (en) * | 1982-02-26 | 1984-11-27 | Massachusetts Institute Of Technology | Tissue-equivalent and method for preparation thereof |
| US4485097A (en) * | 1982-05-26 | 1984-11-27 | Massachusetts Institute Of Technology | Bone-equivalent and method for preparation thereof |
| FR2558337B1 (fr) * | 1984-01-19 | 1986-05-02 | Air Liquide | Dispositif de congelation de produits biologiques conditionnes en paillettes |
| US5084377A (en) * | 1984-09-19 | 1992-01-28 | Larry Rowan | Cryogenic suspension method |
| US4604346A (en) * | 1984-10-09 | 1986-08-05 | Massachusetts Institute Of Technology | Skin-equivalent prepared by the use of punch biopsy |
| US5026649A (en) * | 1986-03-20 | 1991-06-25 | Costar Corporation | Apparatus for growing tissue cultures in vitro |
| US4890457A (en) * | 1987-01-02 | 1990-01-02 | Cryolife, Inc. | Method for cryopreserving heart valves |
| IT1207525B (it) * | 1987-06-23 | 1989-05-25 | Ist Naz Ric Sul Cancro | Metodo per la preservazione difogli trapiantabili di epitelio coltivato in vitro vitale. |
| US4978540A (en) * | 1988-01-20 | 1990-12-18 | Lee Tung Ching | Production of frozen foods and other products |
| US5194269A (en) * | 1988-01-20 | 1993-03-16 | Lee Tung Ching | Production of frozen foods and other products |
| US4837379A (en) * | 1988-06-02 | 1989-06-06 | Organogenesis Inc. | Fibrin-collagen tissue equivalents and methods for preparation thereof |
| CA1335723C (fr) * | 1988-08-04 | 1995-05-30 | Thomas Glonek | Methode et composition pour la cryoconservation de tissue |
| CA2000181A1 (fr) * | 1988-10-14 | 1990-04-14 | Chung-Faye Chao | Methode de culture de lambeaux d'epiderme, produit et methode |
| JPH0651601B2 (ja) * | 1988-10-27 | 1994-07-06 | 伊藤忠飼料株式会社 | 豚の凍結受精卵及び凍結保存方法 |
| JPH02300101A (ja) * | 1989-05-12 | 1990-12-12 | Tabai Espec Corp | 生物試料凍結保存装置における植氷方法および植氷装置 |
| US4988302A (en) * | 1989-06-08 | 1991-01-29 | Difco Laboratories Incorporated | Culture media package |
| US5131850A (en) * | 1989-11-03 | 1992-07-21 | Cryolife, Inc. | Method for cryopreserving musculoskeletal tissues |
| JPH05504052A (ja) * | 1989-11-20 | 1993-07-01 | セル・システムズ・リミテッド | 冷却過程と装置 |
| US5145770A (en) * | 1990-06-04 | 1992-09-08 | Biosurface Technology, Inc. | Cryopreservation of cultured epithelial sheets |
| US5040677A (en) * | 1990-06-04 | 1991-08-20 | Biosurface Technology, Inc. | Container for storage and distribution of a skin wound dressing |
| US5336616A (en) * | 1990-09-12 | 1994-08-09 | Lifecell Corporation | Method for processing and preserving collagen-based tissues for transplantation |
| JPH04365434A (ja) * | 1991-06-12 | 1992-12-17 | Oji Paper Co Ltd | 不定根および/または毛状根の凍結保存法 |
| JP3672201B2 (ja) * | 1991-12-26 | 2005-07-20 | 雪印乳業株式会社 | 哺乳動物胚移植用ストロー及びその製造法 |
| US5366893A (en) * | 1993-01-13 | 1994-11-22 | Becton, Dickinson And Company | Culture vessel |
| US5518878A (en) * | 1993-09-15 | 1996-05-21 | Organogenesis Inc. | Cryopreservation of cultured skin or cornea equivalents with agitation |
| US5891617A (en) * | 1993-09-15 | 1999-04-06 | Organogenesis Inc. | Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing |
| US5689961A (en) * | 1996-01-30 | 1997-11-25 | Organogenesis Inc. | Ice seeding apparatus for cryopreservation systems |
-
1995
- 1995-01-30 US US08/380,099 patent/US5891617A/en not_active Expired - Lifetime
-
1996
- 1996-01-30 CN CNB031457983A patent/CN1292651C/zh not_active Expired - Fee Related
- 1996-01-30 CZ CZ19972413A patent/CZ294950B6/cs not_active IP Right Cessation
- 1996-01-30 WO PCT/US1996/001217 patent/WO1996024018A1/fr not_active Ceased
- 1996-01-30 AT AT04077072T patent/ATE376356T1/de not_active IP Right Cessation
- 1996-01-30 PL PL96321089A patent/PL181762B1/pl not_active IP Right Cessation
- 1996-01-30 EP EP04077072A patent/EP1516532B1/fr not_active Expired - Lifetime
- 1996-01-30 NZ NZ302913A patent/NZ302913A/en unknown
- 1996-01-30 SK SK1033-97A patent/SK284801B6/sk unknown
- 1996-01-30 HU HU9800411A patent/HU223789B1/hu not_active IP Right Cessation
- 1996-01-30 RU RU97116150/13A patent/RU2178865C2/ru not_active IP Right Cessation
- 1996-01-30 SG SG9802806A patent/SG106553A1/en unknown
- 1996-01-30 CN CN96191673A patent/CN1119599C/zh not_active Expired - Fee Related
- 1996-01-30 EP EP96905275A patent/EP0807234B1/fr not_active Expired - Lifetime
- 1996-01-30 ES ES96905275T patent/ES2231804T3/es not_active Expired - Lifetime
- 1996-01-30 AU AU49082/96A patent/AU716869B2/en not_active Ceased
- 1996-01-30 PT PT96905275T patent/PT807234E/pt unknown
- 1996-01-30 JP JP8523665A patent/JPH11501298A/ja not_active Withdrawn
- 1996-01-30 BR BR9606864A patent/BR9606864A/pt not_active IP Right Cessation
- 1996-01-30 DE DE69637301T patent/DE69637301T2/de not_active Expired - Lifetime
- 1996-01-30 DE DE69633854T patent/DE69633854T2/de not_active Expired - Lifetime
- 1996-01-30 KR KR1019970705324A patent/KR100328742B1/ko not_active Expired - Fee Related
- 1996-01-30 AT AT96905275T patent/ATE282805T1/de not_active IP Right Cessation
-
1997
- 1997-06-16 FI FI972563A patent/FI113694B/fi active
- 1997-07-28 NO NO19973463A patent/NO310491B1/no unknown
-
2006
- 2006-07-10 JP JP2006188993A patent/JP4361550B2/ja not_active Expired - Fee Related
-
2007
- 2007-01-04 JP JP2007000107A patent/JP2007161723A/ja not_active Withdrawn
-
2013
- 2013-12-27 JP JP2013272171A patent/JP2014065736A/ja active Pending
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5891617A (en) | Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing | |
| US5964096A (en) | Method and package design for cryopreservation and storage of cultured tissue equivalents | |
| KR100361357B1 (ko) | 배양된 조직등가물의 동결보존법 | |
| JP2722134B2 (ja) | 培養上皮細胞シートの凍結保存 | |
| AU2019232795B2 (en) | Cryopreservation of viable human skin substitutes | |
| CN112638157B (zh) | 一种防止样品和细胞外冰直接接触的有效低温保存装置 | |
| CN111657267B (zh) | 一种用于软骨,肌腱,半月板保存的无冰晶冷冻保存液和冷冻方法 | |
| Zieger et al. | Mechanisms of cryoinjury and cryoprotection in split-thickness skin | |
| JPH09110601A (ja) | 培養上皮細胞シートの凍結保存 | |
| CA2210532C (fr) | Procede et structure d'emballage pour la conservation et le stockage cryogeniques d'equivalents de tissu de culture | |
| Mazur | Principles of medical cryobiology: The freezing of living cells, tissues, and organs |