OA10791A - Vaccines against infections caused by yf virus yf infectious cdna method for producing a recombinantyf virus from the yf infectious cdna and plasmids to assemble the yf infectious cdna - Google Patents

Vaccines against infections caused by yf virus yf infectious cdna method for producing a recombinantyf virus from the yf infectious cdna and plasmids to assemble the yf infectious cdna Download PDF

Info

Publication number: OA10791A
Authority: OA; OAPI
Prior art keywords: virus; cdna; infectious cdna; recombinant; infectious
Prior art date: 1997-04-11

Application number

OA9800041A

Other languages

English (en)

Inventor

Ricardo Galler

Marcos Da Silva Freire

Original Assignee

Fundacoa Oswaldo Cruz Fiocruz

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1997-04-11

Filing date

1998-04-09

Publication date

2002-12-24

1998-04-09 Application filed by Fundacoa Oswaldo Cruz Fiocruz filed Critical Fundacoa Oswaldo Cruz Fiocruz

2002-12-24 Publication of OA10791A publication Critical patent/OA10791A/en

Links

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Classifications

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- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
- C12N2770/24121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
- C12N2770/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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OA9800041A 1997-04-11 1998-04-09 Vaccines against infections caused by yf virus yf infectious cdna method for producing a recombinantyf virus from the yf infectious cdna and plasmids to assemble the yf infectious cdna OA10791A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
BRPI9701774A BRPI9701774B8 (pt)	1997-04-11	1997-04-11	cdna mutante e infeccioso do vírus da febre amarela, constructo de dna, vírus recombinante de febre amarela e vacina para humanos contra infecções de febre amarela.

Publications (1)

Publication Number	Publication Date
OA10791A true OA10791A (en)	2002-12-24

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ID=4066904

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
OA9800041A OA10791A (en)	1997-04-11	1998-04-09	Vaccines against infections caused by yf virus yf infectious cdna method for producing a recombinantyf virus from the yf infectious cdna and plasmids to assemble the yf infectious cdna

Country Status (9)

Country	Link
US (2)	US6171854B1 (es)
EP (1)	EP0877086B1 (es)
BR (1)	BRPI9701774B8 (es)
CA (1)	CA2233932C (es)
CU (1)	CU22770A3 (es)
DE (1)	DE69839169T2 (es)
OA (1)	OA10791A (es)
RU (1)	RU98106999A (es)
ZA (1)	ZA982760B (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6962708B1 (en) *	1997-02-28	2005-11-08	Acambis, Inc.	Chimeric flavivirus vaccines
BRPI9701774B8 (pt)	1997-04-11	2015-09-29	Fundação Oswaldo Cruz Fiocruz	cdna mutante e infeccioso do vírus da febre amarela, constructo de dna, vírus recombinante de febre amarela e vacina para humanos contra infecções de febre amarela.
DE19757301A1 (de)	1997-12-22	1999-06-24	Deutsches Primatenzentrum Gmbh	Intra-nasale Gelbfieber-Impfung
US6541011B2 (en)	1998-02-11	2003-04-01	Maxygen, Inc.	Antigen library immunization
GB2372991B (en) *	2001-03-09	2004-11-17	Fiocruz Fundacao Oswaldo Cruz	Flavivirus expression vector
CN1551782A (zh) *	2001-06-01	2004-12-01	��ķ��	嵌合黄病毒载体
KR101074175B1 (ko) *	2002-01-15	2011-10-14	사노피 파스테르 바이오로직스 씨오	플라비바이러스 백신
US20050002968A1 (en) *	2002-01-15	2005-01-06	Monath Thomas P.	Flavivirus vaccines
US20040259224A1 (en) *	2002-05-31	2004-12-23	Farshad Guirakhoo	Tetravalent Dengue vaccines
DK1375512T3 (da) *	2002-06-20	2009-10-26	Pasteur Institut	Infektiös cDNA af en godkendt vaccinestamme af mæslingevirus. Anvendelse til immunogene sammensætninger
WO2004009764A2 (en) *	2002-07-19	2004-01-29	Board Of Regents, The University Of Texas System	Methods and compositions concerning altered yellow fever virus strains
AU2003263853A1 (en) *	2002-08-16	2004-03-03	Board Of Regents The University Of Texas System	Compositions and methods related to flavivirus envelope protein domain iii antigens
WO2004045529A2 (en) *	2002-11-15	2004-06-03	Acambis, Inc.	West nile virus vaccine
CA2576798C (en)	2004-07-27	2014-09-09	Gwong-Jen J. Chang	Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes and methods for their use
BRPI0504945B8 (pt)	2005-10-31	2022-08-02	Fundacao Oswaldo Cruz	Método para a produção de flavivirus recombinante contendo sequências de nucleotídeos codificantes de uma proteína heteróloga, constructo de dna, flavivirus, e, composição vacinal para imunizar contra flavivirus e/ou outros patógenos.
JP4673428B2 (ja)	2006-06-30	2011-04-20	アーベーベー・テヒノロギー・アーゲー	Ｈｖｄｃシステム及びｈｖｄｃシステムの電圧源変換器の制御方法
US7872560B2 (en) *	2007-03-19	2011-01-18	Abc Taiwan Electronics Corp.	Independent planar transformer
EP1982727A1 (en) *	2007-04-17	2008-10-22	Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.	Method for purification of viral proteins
DK2200588T3 (da) *	2007-09-25	2019-07-01	Solubest Ltd	Sammensætninger, som omfatter lipofile aktive forbindelser, og fremgangsmåde til fremstilling deraf
CN101959527B (zh)	2008-03-05	2016-04-20	赛诺菲巴斯德有限公司	一种使含佐剂的疫苗组合物稳定的方法
EP2143440A1 (fr)	2008-07-09	2010-01-13	Sanofi Pasteur	Agent stabilisant et composition vaccinale comprenant un ou plusieurs flavivirus vivants atténués
ES2544702T3 (es) *	2008-07-17	2015-09-02	Medigen, Inc.	Vacunas en forma de ADNi y métodos para utilizarlas
BRPI0904020B8 (pt)	2009-10-01	2021-05-25	Fundacao Oswaldo Cruz	composição vacinal contra o vírus da dengue, e, kit
BRPI0905645B8 (pt) *	2009-10-27	2021-05-25	Fundacao Oswaldo Cruz	vacina de dna contra o vírus da febre amarela
US11459619B2 (en)	2016-02-08	2022-10-04	The Johns Hopkins University	Handheld nucleic acid-based assay for rapid identification

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4719177A (en)	1981-04-20	1988-01-12	Massachusetts Institute Of Technology	Production of complementary DNA representing RNA viral sequences by recombinant DNA methods and uses therefor
EP1018556B1 (en)	1991-09-19	2005-11-23	THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the Secretary of the Department of HEALTH AND HUMAN SERVICES	Chimeric and/or growth-restricted flaviviruses
BRPI9701774B8 (pt)	1997-04-11	2015-09-29	Fundação Oswaldo Cruz Fiocruz	cdna mutante e infeccioso do vírus da febre amarela, constructo de dna, vírus recombinante de febre amarela e vacina para humanos contra infecções de febre amarela.

1997
- 1997-04-11 BR BRPI9701774A patent/BRPI9701774B8/pt not_active IP Right Cessation
1998
- 1998-04-01 ZA ZA982760A patent/ZA982760B/xx unknown
- 1998-04-07 CA CA002233932A patent/CA2233932C/en not_active Expired - Fee Related
- 1998-04-08 DE DE69839169T patent/DE69839169T2/de not_active Expired - Lifetime
- 1998-04-08 EP EP98106495A patent/EP0877086B1/en not_active Expired - Lifetime
- 1998-04-09 OA OA9800041A patent/OA10791A/en unknown
- 1998-04-10 RU RU98106999/13A patent/RU98106999A/ru not_active Application Discontinuation
- 1998-04-10 US US09/058,411 patent/US6171854B1/en not_active Expired - Lifetime
- 1998-04-10 CU CU1998046A patent/CU22770A3/es unknown
2000
- 2000-11-06 US US09/705,949 patent/US6589522B1/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
CU22770A3 (es)	2002-07-24
ZA982760B (en)	1999-01-18
US6589522B1 (en)	2003-07-08
DE69839169D1 (de)	2008-04-10
BRPI9701774B1 (pt)	2015-08-25
US6171854B1 (en)	2001-01-09
EP0877086A2 (en)	1998-11-11
CA2233932C (en)	2009-01-13
EP0877086A3 (en)	1999-09-01
DE69839169T2 (de)	2009-03-19
EP0877086B1 (en)	2008-02-27
RU98106999A (ru)	2000-02-20
BR9701774A (pt)	2000-08-01
BRPI9701774B8 (pt)	2015-09-29
CA2233932A1 (en)	1998-10-11

Publication	Publication Date	Title
OA10791A (en)	2002-12-24	Vaccines against infections caused by yf virus yf infectious cdna method for producing a recombinantyf virus from the yf infectious cdna and plasmids to assemble the yf infectious cdna
JP3681358B2 (ja)	2005-08-10	キメラおよび／または増殖制限されたフラビウイルス
US8691550B2 (en)	2014-04-08	Vaccines against japanese encephalitis virus and west nile virus
US8088391B2 (en)	2012-01-03	West nile virus vaccine
US7569383B2 (en)	2009-08-04	Chimeric flavivirus vectors
JP6486827B2 (ja)	2019-03-20	デングウイルス血清型４（ｄｅｎ−４）構築物に関する核酸分子、組成物および使用
CA2432370C (en)	2012-01-31	Attenuated flaviviral live vaccine comprising a capsid protein deletion
ES2287074T3 (es)	2007-12-16	Molecula de rna autorreplicante del virus de la hepatitis c.
US20030194801A1 (en)	2003-10-16	Use of flavivirus for the expression of protein epitopes and development of new live attenuated vaccine virus to immune against flavivirus and other infectious agents
AU2006338210A1 (en)	2007-08-23	RNA virus vaccines and methods
Nath et al.	2020	Evaluation of Japanese encephalitis virus E and NS1 proteins immunogenicity using a recombinant Newcastle disease virus in mice
CN101084010B (zh)	2015-05-06	抗日本脑炎病毒及西尼罗病毒的疫苗
MXPA98002801A (es)	1999-05-31	Vacunas contra infecciones provocadas por virus yf;cdna infeccioso de yf, método para producir un virus yf recombinante a partir del cdna infeccioso de yf y plásmidos para juntar el cdna infeccioso de yf
HK1106723B (en)	2015-10-30	Vaccines against japanese encephalitis virus and west nile virus
HK1083192B (en)	2010-09-10	West nile virus vaccine