PL162317B1 - Method for controlling transcription of a chemically controlled gene in plant material - Google Patents

Method for controlling transcription of a chemically controlled gene in plant material

Info

Publication number: PL162317B1
Authority: PL; Poland
Prior art keywords: gene; plant; dna; sequence; dna sequence
Prior art date: 1988-03-08

Application number

PL89278117A

Other languages

English (en)

Polish (pl)

Other versions

PL278117A1 (en

Original Assignee

Ciba Geigy Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-03-08

Filing date

1989-03-07

Publication date

1993-09-30

1989-03-07 Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag

1989-11-27 Publication of PL278117A1 publication Critical patent/PL278117A1/xx

1993-09-30 Publication of PL162317B1 publication Critical patent/PL162317B1/pl

Links

108090000623 proteins and genes Proteins 0.000 title claims abstract description 385
238000000034 method Methods 0.000 title claims abstract description 159
238000013518 transcription Methods 0.000 title claims abstract description 48
230000035897 transcription Effects 0.000 title claims abstract description 48
239000000463 material Substances 0.000 title claims abstract description 19
108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 285
230000001105 regulatory effect Effects 0.000 claims abstract description 104
239000000126 substance Substances 0.000 claims abstract description 101
230000014509 gene expression Effects 0.000 claims abstract description 78
108091032973 (ribonucleotides)n+m Proteins 0.000 claims abstract description 72
230000033228 biological regulation Effects 0.000 claims abstract description 34
108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 25
102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 18
230000001419 dependent effect Effects 0.000 claims abstract description 17
230000000692 anti-sense effect Effects 0.000 claims abstract description 11
229920001184 polypeptide Polymers 0.000 claims abstract description 11
238000012216 screening Methods 0.000 claims abstract description 7
230000003213 activating effect Effects 0.000 claims abstract description 3
108020004414 DNA Proteins 0.000 claims description 173
210000001938 protoplast Anatomy 0.000 claims description 118
210000004027 cell Anatomy 0.000 claims description 89
239000002299 complementary DNA Substances 0.000 claims description 56
230000009466 transformation Effects 0.000 claims description 55
239000000523 sample Substances 0.000 claims description 37
102000004190 Enzymes Human genes 0.000 claims description 32
108090000790 Enzymes Proteins 0.000 claims description 32
101000611441 Solanum lycopersicum Pathogenesis-related leaf protein 6 Proteins 0.000 claims description 31
239000004009 herbicide Substances 0.000 claims description 30
241000193388 Bacillus thuringiensis Species 0.000 claims description 28
229940097012 bacillus thuringiensis Drugs 0.000 claims description 28
230000001939 inductive effect Effects 0.000 claims description 28
239000003550 marker Substances 0.000 claims description 26
230000002363 herbicidal effect Effects 0.000 claims description 25
238000004519 manufacturing process Methods 0.000 claims description 19
WQZZXEIOGYIXAZ-UHFFFAOYSA-N 1,2,3-benzothiadiazole-4-carboxylic acid Chemical compound OC(=O)C1=CC=CC2=C1N=NS2 WQZZXEIOGYIXAZ-UHFFFAOYSA-N 0.000 claims description 18
241000589158 Agrobacterium Species 0.000 claims description 18
238000002955 isolation Methods 0.000 claims description 17
239000002158 endotoxin Substances 0.000 claims description 16
244000052769 pathogen Species 0.000 claims description 15
108010025815 Kanamycin Kinase Proteins 0.000 claims description 14
WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 14
YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims description 14
108010000700 Acetolactate synthase Proteins 0.000 claims description 13
238000012546 transfer Methods 0.000 claims description 13
108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 claims description 12
102000003960 Ligases Human genes 0.000 claims description 12
108090000364 Ligases Proteins 0.000 claims description 12
238000011161 development Methods 0.000 claims description 10
230000001717 pathogenic effect Effects 0.000 claims description 9
230000008569 process Effects 0.000 claims description 9
230000001131 transforming effect Effects 0.000 claims description 9
229920002125 Sokalan® Polymers 0.000 claims description 8
239000004584 polyacrylic acid Substances 0.000 claims description 8
FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims description 7
229960004889 salicylic acid Drugs 0.000 claims description 7
239000000411 inducer Substances 0.000 claims description 6
239000005711 Benzoic acid Substances 0.000 claims description 5
241000607479 Yersinia pestis Species 0.000 claims description 5
235000010233 benzoic acid Nutrition 0.000 claims description 5
230000001404 mediated effect Effects 0.000 claims description 5
229930000044 secondary metabolite Natural products 0.000 claims description 5
ADNVINJYCACQDG-UHFFFAOYSA-N 1,2,3-benzothiadiazole-4-carbohydrazide Chemical compound NNC(=O)C1=CC=CC2=C1N=NS2 ADNVINJYCACQDG-UHFFFAOYSA-N 0.000 claims description 4
NHPZGJNCBBVUHW-UHFFFAOYSA-N 1,2,3-benzothiadiazole-4-carboxamide Chemical compound NC(=O)C1=CC=CC2=C1N=NS2 NHPZGJNCBBVUHW-UHFFFAOYSA-N 0.000 claims description 4
108060001084 Luciferase Proteins 0.000 claims description 4
239000005089 Luciferase Substances 0.000 claims description 4
238000000520 microinjection Methods 0.000 claims description 4
QKJURJAPCGTFOF-UHFFFAOYSA-N 1,2,3-benzothiadiazole-4-carbonitrile Chemical compound N#CC1=CC=CC2=C1N=NS2 QKJURJAPCGTFOF-UHFFFAOYSA-N 0.000 claims description 3
RHAMZQRZFUCHFE-UHFFFAOYSA-N 1,2,3-benzothiadiazole-4-carbothioic s-acid Chemical compound SC(=O)C1=CC=CC2=C1N=NS2 RHAMZQRZFUCHFE-UHFFFAOYSA-N 0.000 claims description 3
BUQPTOSHKHYHHB-UHFFFAOYSA-N 3,5-dichloropyridine-4-carboxylic acid Chemical compound OC(=O)C1=C(Cl)C=NC=C1Cl BUQPTOSHKHYHHB-UHFFFAOYSA-N 0.000 claims description 3
IMXSCCDUAFEIOE-UHFFFAOYSA-N D-Octopin Natural products OC(=O)C(C)NC(C(O)=O)CCCN=C(N)N IMXSCCDUAFEIOE-UHFFFAOYSA-N 0.000 claims description 3
101150038105 pr gene Proteins 0.000 claims description 3
230000036512 infertility Effects 0.000 claims description 2
238000011426 transformation method Methods 0.000 claims description 2
BSYNRYMUTXBXSQ-FOQJRBATSA-N 59096-14-9 Chemical compound CC(=O)OC1=CC=CC=C1[14C](O)=O BSYNRYMUTXBXSQ-FOQJRBATSA-N 0.000 claims 2
229960004365 benzoic acid Drugs 0.000 claims 2
208000035240 Disease Resistance Diseases 0.000 claims 1
230000007246 mechanism Effects 0.000 abstract description 14
241000196324 Embryophyta Species 0.000 description 297
239000013612 plasmid Substances 0.000 description 230
239000012634 fragment Substances 0.000 description 132
210000001519 tissue Anatomy 0.000 description 127
239000002609 medium Substances 0.000 description 79
244000061176 Nicotiana tabacum Species 0.000 description 76
239000000243 solution Substances 0.000 description 76
235000002637 Nicotiana tabacum Nutrition 0.000 description 68
239000013598 vector Substances 0.000 description 66
102000004169 proteins and genes Human genes 0.000 description 64
206010020649 Hyperkeratosis Diseases 0.000 description 59
235000018102 proteins Nutrition 0.000 description 59
229920000936 Agarose Polymers 0.000 description 57
108010060309 Glucuronidase Proteins 0.000 description 48
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 48
108091026890 Coding region Proteins 0.000 description 46
102000053187 Glucuronidase Human genes 0.000 description 45
239000000203 mixture Substances 0.000 description 39
239000000725 suspension Substances 0.000 description 38
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 37
239000013615 primer Substances 0.000 description 35
101000742121 Arabidopsis thaliana Pathogenesis-related protein 1 Proteins 0.000 description 34
101000742139 Cucumis melo Pathogenesis-related protein Proteins 0.000 description 34
230000006698 induction Effects 0.000 description 33
229920001223 polyethylene glycol Polymers 0.000 description 33
239000000047 product Substances 0.000 description 33
239000002202 Polyethylene glycol Substances 0.000 description 32
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 32
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 30
229940088598 enzyme Drugs 0.000 description 30
239000011543 agarose gel Substances 0.000 description 28
239000000872 buffer Substances 0.000 description 28
238000010276 construction Methods 0.000 description 27
239000002253 acid Substances 0.000 description 26
230000000694 effects Effects 0.000 description 26
108091034117 Oligonucleotide Proteins 0.000 description 24
240000008067 Cucumis sativus Species 0.000 description 22
240000008042 Zea mays Species 0.000 description 22
108020004999 messenger RNA Proteins 0.000 description 22
229920001817 Agar Polymers 0.000 description 21
235000010799 Cucumis sativus var sativus Nutrition 0.000 description 21
238000006243 chemical reaction Methods 0.000 description 21
238000002360 preparation method Methods 0.000 description 21
239000008272 agar Substances 0.000 description 20
229930027917 kanamycin Natural products 0.000 description 20
229960000318 kanamycin Drugs 0.000 description 20
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 20
229930182823 kanamycin A Natural products 0.000 description 20
150000001875 compounds Chemical class 0.000 description 19
238000001962 electrophoresis Methods 0.000 description 19
241000894006 Bacteria Species 0.000 description 17
108010022172 Chitinases Proteins 0.000 description 17
229930006000 Sucrose Natural products 0.000 description 17
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 17
229960004793 sucrose Drugs 0.000 description 17
238000005406 washing Methods 0.000 description 17
241000219194 Arabidopsis Species 0.000 description 16
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 16
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 16
108010076504 Protein Sorting Signals Proteins 0.000 description 16
238000004458 analytical method Methods 0.000 description 16
239000000499 gel Substances 0.000 description 16
238000013507 mapping Methods 0.000 description 16
239000011780 sodium chloride Substances 0.000 description 16
239000005720 sucrose Substances 0.000 description 16
230000009261 transgenic effect Effects 0.000 description 16
101000879603 Nicotiana tabacum Acidic endochitinase Q Proteins 0.000 description 15
241000723873 Tobacco mosaic virus Species 0.000 description 14
125000003275 alpha amino acid group Chemical group 0.000 description 14
238000005119 centrifugation Methods 0.000 description 14
239000007788 liquid Substances 0.000 description 14
238000005507 spraying Methods 0.000 description 14
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 13
238000002474 experimental method Methods 0.000 description 13
238000009396 hybridization Methods 0.000 description 13
229910052757 nitrogen Inorganic materials 0.000 description 13
239000002773 nucleotide Substances 0.000 description 13
239000006228 supernatant Substances 0.000 description 13
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 12
GRRNUXAQVGOGFE-UHFFFAOYSA-N Hygromycin-B Natural products OC1C(NC)CC(N)C(O)C1OC1C2OC3(C(C(O)C(O)C(C(N)CO)O3)O)OC2C(O)C(CO)O1 GRRNUXAQVGOGFE-UHFFFAOYSA-N 0.000 description 12
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 12
235000007244 Zea mays Nutrition 0.000 description 12
238000003556 assay Methods 0.000 description 12
230000029087 digestion Effects 0.000 description 12
101150054900 gus gene Proteins 0.000 description 12
GRRNUXAQVGOGFE-NZSRVPFOSA-N hygromycin B Chemical compound O[C@@H]1[C@@H](NC)C[C@@H](N)[C@H](O)[C@H]1O[C@H]1[C@H]2O[C@@]3([C@@H]([C@@H](O)[C@@H](O)[C@@H](C(N)CO)O3)O)O[C@H]2[C@@H](O)[C@@H](CO)O1 GRRNUXAQVGOGFE-NZSRVPFOSA-N 0.000 description 12
229940097277 hygromycin b Drugs 0.000 description 12
238000002156 mixing Methods 0.000 description 12
125000003729 nucleotide group Chemical group 0.000 description 12
238000011069 regeneration method Methods 0.000 description 12
239000008223 sterile water Substances 0.000 description 12
239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 11
108020005029 5' Flanking Region Proteins 0.000 description 11
240000004585 Dactylis glomerata Species 0.000 description 11
101710163270 Nuclease Proteins 0.000 description 11
230000015572 biosynthetic process Effects 0.000 description 11
238000004520 electroporation Methods 0.000 description 11
239000001963 growth medium Substances 0.000 description 11
230000001965 increasing effect Effects 0.000 description 11
230000008929 regeneration Effects 0.000 description 11
239000000758 substrate Substances 0.000 description 11
241000589155 Agrobacterium tumefaciens Species 0.000 description 10
238000002965 ELISA Methods 0.000 description 10
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 10
241000238631 Hexapoda Species 0.000 description 10
101000706020 Nicotiana tabacum Pathogenesis-related protein R minor form Proteins 0.000 description 10
108091092724 Noncoding DNA Proteins 0.000 description 10
DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 10
235000002017 Zea mays subsp mays Nutrition 0.000 description 10
230000008901 benefit Effects 0.000 description 10
244000309466 calf Species 0.000 description 10
230000000875 corresponding effect Effects 0.000 description 10
238000001914 filtration Methods 0.000 description 10
239000008103 glucose Substances 0.000 description 10
-1 heat shock Substances 0.000 description 10
208000015181 infectious disease Diseases 0.000 description 10
210000004196 psta Anatomy 0.000 description 10
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 9
102000012286 Chitinases Human genes 0.000 description 9
235000002767 Daucus carota Nutrition 0.000 description 9
244000000626 Daucus carota Species 0.000 description 9
241000588724 Escherichia coli Species 0.000 description 9
108700009124 Transcription Initiation Site Proteins 0.000 description 9
239000007983 Tris buffer Substances 0.000 description 9
238000010367 cloning Methods 0.000 description 9
230000018109 developmental process Effects 0.000 description 9
239000012528 membrane Substances 0.000 description 9
239000002244 precipitate Substances 0.000 description 9
150000003839 salts Chemical class 0.000 description 9
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 9
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 8
229920000742 Cotton Polymers 0.000 description 8
238000001712 DNA sequencing Methods 0.000 description 8
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 8
YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 8
244000299507 Gossypium hirsutum Species 0.000 description 8
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 8
240000003768 Solanum lycopersicum Species 0.000 description 8
125000000217 alkyl group Chemical group 0.000 description 8
235000001014 amino acid Nutrition 0.000 description 8
238000005516 engineering process Methods 0.000 description 8
239000000284 extract Substances 0.000 description 8
239000012737 fresh medium Substances 0.000 description 8
238000011534 incubation Methods 0.000 description 8
150000004702 methyl esters Chemical class 0.000 description 8
238000012986 modification Methods 0.000 description 8
230000004048 modification Effects 0.000 description 8
SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 8
FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 7
229930195725 Mannitol Natural products 0.000 description 7
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 7
229940024606 amino acid Drugs 0.000 description 7
150000001413 amino acids Chemical class 0.000 description 7
230000003115 biocidal effect Effects 0.000 description 7
FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 7
229960003669 carbenicillin Drugs 0.000 description 7
230000000408 embryogenic effect Effects 0.000 description 7
239000000594 mannitol Substances 0.000 description 7
235000010355 mannitol Nutrition 0.000 description 7
108010058731 nopaline synthase Proteins 0.000 description 7
239000008188 pellet Substances 0.000 description 7
108091008146 restriction endonucleases Proteins 0.000 description 7
239000007787 solid Substances 0.000 description 7
238000010561 standard procedure Methods 0.000 description 7
238000012360 testing method Methods 0.000 description 7
210000001541 thymus gland Anatomy 0.000 description 7
238000013519 translation Methods 0.000 description 7
FNQJDLTXOVEEFB-UHFFFAOYSA-N 1,2,3-benzothiadiazole Chemical compound C1=CC=C2SN=NC2=C1 FNQJDLTXOVEEFB-UHFFFAOYSA-N 0.000 description 6
108010066133 D-octopine dehydrogenase Proteins 0.000 description 6
102100031780 Endonuclease Human genes 0.000 description 6
235000010469 Glycine max Nutrition 0.000 description 6
235000003222 Helianthus annuus Nutrition 0.000 description 6
244000020551 Helianthus annuus Species 0.000 description 6
235000007688 Lycopersicon esculentum Nutrition 0.000 description 6
101000763602 Manilkara zapota Thaumatin-like protein 1 Proteins 0.000 description 6
101000763586 Manilkara zapota Thaumatin-like protein 1a Proteins 0.000 description 6
101100464974 Medicago truncatula PR-1 gene Proteins 0.000 description 6
101000966653 Musa acuminata Glucan endo-1,3-beta-glucosidase Proteins 0.000 description 6
241000208125 Nicotiana Species 0.000 description 6
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 6
241000700605 Viruses Species 0.000 description 6
235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 6
230000009471 action Effects 0.000 description 6
238000004113 cell culture Methods 0.000 description 6
239000003795 chemical substances by application Substances 0.000 description 6
230000000295 complement effect Effects 0.000 description 6
230000004927 fusion Effects 0.000 description 6
238000002372 labelling Methods 0.000 description 6
229910001629 magnesium chloride Inorganic materials 0.000 description 6
235000009973 maize Nutrition 0.000 description 6
239000000843 powder Substances 0.000 description 6
RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 6
230000035939 shock Effects 0.000 description 6
DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 6
239000011550 stock solution Substances 0.000 description 6
235000004977 Brassica sinapistrum Nutrition 0.000 description 5
241000701959 Escherichia virus Lambda Species 0.000 description 5
229920002148 Gellan gum Polymers 0.000 description 5
244000068988 Glycine max Species 0.000 description 5
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 5
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 5
230000002378 acidificating effect Effects 0.000 description 5
125000003545 alkoxy group Chemical group 0.000 description 5
238000000376 autoradiography Methods 0.000 description 5
239000011575 calcium Substances 0.000 description 5
239000005018 casein Substances 0.000 description 5
BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 5
235000021240 caseins Nutrition 0.000 description 5
235000021186 dishes Nutrition 0.000 description 5
210000002257 embryonic structure Anatomy 0.000 description 5
230000002255 enzymatic effect Effects 0.000 description 5
241001233957 eudicotyledons Species 0.000 description 5
238000000605 extraction Methods 0.000 description 5
238000010353 genetic engineering Methods 0.000 description 5
238000000227 grinding Methods 0.000 description 5
230000012010 growth Effects 0.000 description 5
239000004570 mortar (masonry) Substances 0.000 description 5
229920003023 plastic Polymers 0.000 description 5
239000004033 plastic Substances 0.000 description 5
238000000746 purification Methods 0.000 description 5
238000011160 research Methods 0.000 description 5
125000001424 substituent group Chemical group 0.000 description 5
238000003786 synthesis reaction Methods 0.000 description 5
SQSYNRCXIZHKAI-UHFFFAOYSA-N 2,6-dichloroisonicotinic acid Chemical compound OC(=O)C1=CC(Cl)=NC(Cl)=C1 SQSYNRCXIZHKAI-UHFFFAOYSA-N 0.000 description 4
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
ROWKJAVDOGWPAT-UHFFFAOYSA-N Acetoin Chemical compound CC(O)C(C)=O ROWKJAVDOGWPAT-UHFFFAOYSA-N 0.000 description 4
ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 4
241000589156 Agrobacterium rhizogenes Species 0.000 description 4
102000002260 Alkaline Phosphatase Human genes 0.000 description 4
108020004774 Alkaline Phosphatase Proteins 0.000 description 4
JFPVXVDWJQMJEE-QMTHXVAHSA-N Cefuroxime Chemical compound N([C@@H]1C(N2C(=C(COC(N)=O)CS[C@@H]21)C(O)=O)=O)C(=O)C(=NOC)C1=CC=CO1 JFPVXVDWJQMJEE-QMTHXVAHSA-N 0.000 description 4
102000012410 DNA Ligases Human genes 0.000 description 4
108010061982 DNA Ligases Proteins 0.000 description 4
239000005504 Dicamba Substances 0.000 description 4
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
241000233866 Fungi Species 0.000 description 4
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
244000100545 Lolium multiflorum Species 0.000 description 4
208000007466 Male Infertility Diseases 0.000 description 4
240000004658 Medicago sativa Species 0.000 description 4
206010028980 Neoplasm Diseases 0.000 description 4
241000219000 Populus Species 0.000 description 4
CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
229940100389 Sulfonylurea Drugs 0.000 description 4
KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 4
230000002411 adverse Effects 0.000 description 4
125000005907 alkyl ester group Chemical group 0.000 description 4
125000004414 alkyl thio group Chemical group 0.000 description 4
125000003118 aryl group Chemical group 0.000 description 4
230000001580 bacterial effect Effects 0.000 description 4
238000012512 characterization method Methods 0.000 description 4
239000003153 chemical reaction reagent Substances 0.000 description 4
229960005091 chloramphenicol Drugs 0.000 description 4
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 4
DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 4
238000005520 cutting process Methods 0.000 description 4
IWEDIXLBFLAXBO-UHFFFAOYSA-N dicamba Chemical compound COC1=C(Cl)C=CC(Cl)=C1C(O)=O IWEDIXLBFLAXBO-UHFFFAOYSA-N 0.000 description 4
238000010828 elution Methods 0.000 description 4
229910052736 halogen Inorganic materials 0.000 description 4
150000002367 halogens Chemical class 0.000 description 4
238000004128 high performance liquid chromatography Methods 0.000 description 4
239000000543 intermediate Substances 0.000 description 4
210000002977 intracellular fluid Anatomy 0.000 description 4
229940087515 mefoxin Drugs 0.000 description 4
XSKGHSUHOYEBTK-UHFFFAOYSA-N methyl 2,6-dichloropyridine-4-carboxylate Chemical compound COC(=O)C1=CC(Cl)=NC(Cl)=C1 XSKGHSUHOYEBTK-UHFFFAOYSA-N 0.000 description 4
YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 4
239000008363 phosphate buffer Substances 0.000 description 4
239000011541 reaction mixture Substances 0.000 description 4
238000000926 separation method Methods 0.000 description 4
238000012163 sequencing technique Methods 0.000 description 4
229910000162 sodium phosphate Inorganic materials 0.000 description 4
239000002689 soil Substances 0.000 description 4
238000004659 sterilization and disinfection Methods 0.000 description 4
COAIOOWBEPAOFY-UHFFFAOYSA-N 1,2,3-benzothiadiazole-7-carboxylic acid Chemical compound OC(=O)C1=CC=CC2=C1SN=N2 COAIOOWBEPAOFY-UHFFFAOYSA-N 0.000 description 3
241000193830 Bacillus <bacterium> Species 0.000 description 3
240000002791 Brassica napus Species 0.000 description 3
108010059892 Cellulase Proteins 0.000 description 3
102000053602 DNA Human genes 0.000 description 3
102000004594 DNA Polymerase I Human genes 0.000 description 3
108010017826 DNA Polymerase I Proteins 0.000 description 3
206010020751 Hypersensitivity Diseases 0.000 description 3
206010021928 Infertility female Diseases 0.000 description 3
206010021929 Infertility male Diseases 0.000 description 3
FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 3
101001018085 Lysobacter enzymogenes Lysyl endopeptidase Proteins 0.000 description 3
235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 3
NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 3
229920002274 Nalgene Polymers 0.000 description 3
241000208133 Nicotiana plumbaginifolia Species 0.000 description 3
240000007594 Oryza sativa Species 0.000 description 3
235000007164 Oryza sativa Nutrition 0.000 description 3
240000007377 Petunia x hybrida Species 0.000 description 3
108020004511 Recombinant DNA Proteins 0.000 description 3
240000004808 Saccharomyces cerevisiae Species 0.000 description 3
238000012300 Sequence Analysis Methods 0.000 description 3
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 description 3
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
238000000246 agarose gel electrophoresis Methods 0.000 description 3
239000003242 anti bacterial agent Substances 0.000 description 3
239000008346 aqueous phase Substances 0.000 description 3
230000009286 beneficial effect Effects 0.000 description 3
UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 3
150000001732 carboxylic acid derivatives Chemical class 0.000 description 3
230000001413 cellular effect Effects 0.000 description 3
229940106157 cellulase Drugs 0.000 description 3
239000013043 chemical agent Substances 0.000 description 3
239000007795 chemical reaction product Substances 0.000 description 3
235000005822 corn Nutrition 0.000 description 3
125000004093 cyano group Chemical group *C#N 0.000 description 3
230000006378 damage Effects 0.000 description 3
238000012217 deletion Methods 0.000 description 3
230000037430 deletion Effects 0.000 description 3
239000000975 dye Substances 0.000 description 3
235000013399 edible fruits Nutrition 0.000 description 3
239000000839 emulsion Substances 0.000 description 3
238000009472 formulation Methods 0.000 description 3
238000001502 gel electrophoresis Methods 0.000 description 3
230000002068 genetic effect Effects 0.000 description 3
230000001976 improved effect Effects 0.000 description 3
230000006872 improvement Effects 0.000 description 3
238000003780 insertion Methods 0.000 description 3
230000037431 insertion Effects 0.000 description 3
230000003993 interaction Effects 0.000 description 3
229960000485 methotrexate Drugs 0.000 description 3
CUIIHNPMMCYAMK-UHFFFAOYSA-N methyl 1,2,3-benzothiadiazole-7-carboxylate Chemical compound COC(=O)C1=CC=CC2=C1SN=N2 CUIIHNPMMCYAMK-UHFFFAOYSA-N 0.000 description 3
239000011785 micronutrient Substances 0.000 description 3
235000013369 micronutrients Nutrition 0.000 description 3
230000000877 morphologic effect Effects 0.000 description 3
125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 description 3
229910052760 oxygen Inorganic materials 0.000 description 3
230000036961 partial effect Effects 0.000 description 3
239000002245 particle Substances 0.000 description 3
239000012071 phase Substances 0.000 description 3
239000003375 plant hormone Substances 0.000 description 3
229950010131 puromycin Drugs 0.000 description 3
230000003362 replicative effect Effects 0.000 description 3
230000001718 repressive effect Effects 0.000 description 3
238000004007 reversed phase HPLC Methods 0.000 description 3
235000009566 rice Nutrition 0.000 description 3
239000012266 salt solution Substances 0.000 description 3
239000001632 sodium acetate Substances 0.000 description 3
235000017281 sodium acetate Nutrition 0.000 description 3
229940054269 sodium pyruvate Drugs 0.000 description 3
229960004025 sodium salicylate Drugs 0.000 description 3
229910001220 stainless steel Inorganic materials 0.000 description 3
239000010935 stainless steel Substances 0.000 description 3
230000001954 sterilising effect Effects 0.000 description 3
YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical class OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 3
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 3
230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 3
229940088594 vitamin Drugs 0.000 description 3
235000013343 vitamin Nutrition 0.000 description 3
239000011782 vitamin Substances 0.000 description 3
229930003231 vitamin Natural products 0.000 description 3
239000011534 wash buffer Substances 0.000 description 3
239000004563 wettable powder Substances 0.000 description 3
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 2
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
241000239223 Arachnida Species 0.000 description 2
101710130006 Beta-glucanase Proteins 0.000 description 2
108010006654 Bleomycin Proteins 0.000 description 2
108091003079 Bovine Serum Albumin Proteins 0.000 description 2
235000014698 Brassica juncea var multisecta Nutrition 0.000 description 2
235000006008 Brassica napus var napus Nutrition 0.000 description 2
235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 2
244000188595 Brassica sinapistrum Species 0.000 description 2
LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 2
241000218631 Coniferophyta Species 0.000 description 2
241001131785 Escherichia coli HB101 Species 0.000 description 2
108091029865 Exogenous DNA Proteins 0.000 description 2
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Natural products NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
108010004889 Heat-Shock Proteins Proteins 0.000 description 2
240000005979 Hordeum vulgare Species 0.000 description 2
235000007340 Hordeum vulgare Nutrition 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
101150031639 IV gene Proteins 0.000 description 2
FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 2
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
SNDPXSYFESPGGJ-UHFFFAOYSA-N L-norVal-OH Natural products CCCC(N)C(O)=O SNDPXSYFESPGGJ-UHFFFAOYSA-N 0.000 description 2
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
OJMMVQQUTAEWLP-UHFFFAOYSA-N Lincomycin Natural products CN1CC(CCC)CC1C(=O)NC(C(C)O)C1C(O)C(O)C(O)C(SC)O1 OJMMVQQUTAEWLP-UHFFFAOYSA-N 0.000 description 2
241001465754 Metazoa Species 0.000 description 2
DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
MDRASYOETDBPOH-UHFFFAOYSA-N O=C(C1=C2N=NSC2=CC=C1)OCC1=CC=CC=C1 Chemical compound O=C(C1=C2N=NSC2=CC=C1)OCC1=CC=CC=C1 MDRASYOETDBPOH-UHFFFAOYSA-N 0.000 description 2
108020005187 Oligonucleotide Probes Proteins 0.000 description 2
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 2
OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
108700001094 Plant Genes Proteins 0.000 description 2
108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 2
102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 2
229920001213 Polysorbate 20 Polymers 0.000 description 2
101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 2
229920005654 Sephadex Polymers 0.000 description 2
239000012507 Sephadex™ Substances 0.000 description 2
108020004682 Single-Stranded DNA Proteins 0.000 description 2
244000061458 Solanum melongena Species 0.000 description 2
235000002597 Solanum melongena Nutrition 0.000 description 2
244000061456 Solanum tuberosum Species 0.000 description 2
235000002595 Solanum tuberosum Nutrition 0.000 description 2
240000006394 Sorghum bicolor Species 0.000 description 2
235000007230 Sorghum bicolor Nutrition 0.000 description 2
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
239000008049 TAE buffer Substances 0.000 description 2
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
239000007984 Tris EDTA buffer Substances 0.000 description 2
102000004142 Trypsin Human genes 0.000 description 2
108090000631 Trypsin Proteins 0.000 description 2
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
238000009825 accumulation Methods 0.000 description 2
HGEVZDLYZYVYHD-UHFFFAOYSA-N acetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid Chemical compound CC(O)=O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O HGEVZDLYZYVYHD-UHFFFAOYSA-N 0.000 description 2
230000004913 activation Effects 0.000 description 2
150000001298 alcohols Chemical class 0.000 description 2
208000026935 allergic disease Diseases 0.000 description 2
102000006646 aminoglycoside phosphotransferase Human genes 0.000 description 2
235000011114 ammonium hydroxide Nutrition 0.000 description 2
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
229910052921 ammonium sulfate Inorganic materials 0.000 description 2
235000011130 ammonium sulphate Nutrition 0.000 description 2
229960000723 ampicillin Drugs 0.000 description 2
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
239000007864 aqueous solution Substances 0.000 description 2
MXWJVTOOROXGIU-UHFFFAOYSA-N atrazine Chemical compound CCNC1=NC(Cl)=NC(NC(C)C)=N1 MXWJVTOOROXGIU-UHFFFAOYSA-N 0.000 description 2
239000011324 bead Substances 0.000 description 2
125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 2
229960001561 bleomycin Drugs 0.000 description 2
OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 2
229940098773 bovine serum albumin Drugs 0.000 description 2
239000000969 carrier Substances 0.000 description 2
230000015556 catabolic process Effects 0.000 description 2
238000006555 catalytic reaction Methods 0.000 description 2
210000000349 chromosome Anatomy 0.000 description 2
239000000084 colloidal system Substances 0.000 description 2
230000001276 controlling effect Effects 0.000 description 2
229940109239 creatinine Drugs 0.000 description 2
238000012258 culturing Methods 0.000 description 2
OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
238000006731 degradation reaction Methods 0.000 description 2
239000003085 diluting agent Substances 0.000 description 2
238000010790 dilution Methods 0.000 description 2
239000012895 dilution Substances 0.000 description 2
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
238000010410 dusting Methods 0.000 description 2
230000005684 electric field Effects 0.000 description 2
210000002472 endoplasmic reticulum Anatomy 0.000 description 2
238000012869 ethanol precipitation Methods 0.000 description 2
239000013604 expression vector Substances 0.000 description 2
239000011536 extraction buffer Substances 0.000 description 2
VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 description 2
235000019162 flavin adenine dinucleotide Nutrition 0.000 description 2
239000011714 flavin adenine dinucleotide Substances 0.000 description 2
229940093632 flavin-adenine dinucleotide Drugs 0.000 description 2
OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
238000004108 freeze drying Methods 0.000 description 2
230000004345 fruit ripening Effects 0.000 description 2
230000006870 function Effects 0.000 description 2
238000010359 gene isolation Methods 0.000 description 2
BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Natural products O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 2
229930182480 glucuronide Natural products 0.000 description 2
150000008134 glucuronides Chemical class 0.000 description 2
235000011187 glycerol Nutrition 0.000 description 2
125000004438 haloalkoxy group Chemical group 0.000 description 2
125000001188 haloalkyl group Chemical group 0.000 description 2
238000010438 heat treatment Methods 0.000 description 2
239000011539 homogenization buffer Substances 0.000 description 2
GFAZHVHNLUBROE-UHFFFAOYSA-N hydroxymethyl propionaldehyde Natural products CCC(=O)CO GFAZHVHNLUBROE-UHFFFAOYSA-N 0.000 description 2
230000009610 hypersensitivity Effects 0.000 description 2
FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 2
QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 2
229960001669 kinetin Drugs 0.000 description 2
OJMMVQQUTAEWLP-KIDUDLJLSA-N lincomycin Chemical compound CN1C[C@H](CCC)C[C@H]1C(=O)N[C@H]([C@@H](C)O)[C@@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](SC)O1 OJMMVQQUTAEWLP-KIDUDLJLSA-N 0.000 description 2
229960005287 lincomycin Drugs 0.000 description 2
KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 2
230000004807 localization Effects 0.000 description 2
235000021073 macronutrients Nutrition 0.000 description 2
210000001161 mammalian embryo Anatomy 0.000 description 2
238000002844 melting Methods 0.000 description 2
239000002207 metabolite Substances 0.000 description 2
SPARFBXWIOTGCY-UHFFFAOYSA-N methyl 1,2,3-benzothiadiazole-4-carboxylate Chemical compound COC(=O)C1=CC=CC2=C1N=NS2 SPARFBXWIOTGCY-UHFFFAOYSA-N 0.000 description 2
235000019799 monosodium phosphate Nutrition 0.000 description 2
238000002703 mutagenesis Methods 0.000 description 2
231100000350 mutagenesis Toxicity 0.000 description 2
125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 2
230000017074 necrotic cell death Effects 0.000 description 2
230000007935 neutral effect Effects 0.000 description 2
239000002751 oligonucleotide probe Substances 0.000 description 2
210000000056 organ Anatomy 0.000 description 2
230000008506 pathogenesis Effects 0.000 description 2
239000000575 pesticide Substances 0.000 description 2
125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 2
230000008121 plant development Effects 0.000 description 2
230000008635 plant growth Effects 0.000 description 2
238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 2
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
239000002987 primer (paints) Substances 0.000 description 2
229960002429 proline Drugs 0.000 description 2
230000000644 propagated effect Effects 0.000 description 2
230000002285 radioactive effect Effects 0.000 description 2
230000009467 reduction Effects 0.000 description 2
230000001172 regenerating effect Effects 0.000 description 2
238000009877 rendering Methods 0.000 description 2
230000010076 replication Effects 0.000 description 2
230000000754 repressing effect Effects 0.000 description 2
230000005070 ripening Effects 0.000 description 2
238000002741 site-directed mutagenesis Methods 0.000 description 2
235000017550 sodium carbonate Nutrition 0.000 description 2
229910000029 sodium carbonate Inorganic materials 0.000 description 2
AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 2
239000001488 sodium phosphate Substances 0.000 description 2
235000011008 sodium phosphates Nutrition 0.000 description 2
241000894007 species Species 0.000 description 2
UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 2
229960000268 spectinomycin Drugs 0.000 description 2
239000007921 spray Substances 0.000 description 2
239000007858 starting material Substances 0.000 description 2
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
235000000346 sugar Nutrition 0.000 description 2
229910052717 sulfur Inorganic materials 0.000 description 2
235000011149 sulphuric acid Nutrition 0.000 description 2
230000001052 transient effect Effects 0.000 description 2
230000007704 transition Effects 0.000 description 2
239000001226 triphosphate Substances 0.000 description 2
235000011178 triphosphate Nutrition 0.000 description 2
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
239000012588 trypsin Substances 0.000 description 2
230000003612 virological effect Effects 0.000 description 2
LDVVMCZRFWMZSG-OLQVQODUSA-N (3ar,7as)-2-(trichloromethylsulfanyl)-3a,4,7,7a-tetrahydroisoindole-1,3-dione Chemical compound C1C=CC[C@H]2C(=O)N(SC(Cl)(Cl)Cl)C(=O)[C@H]21 LDVVMCZRFWMZSG-OLQVQODUSA-N 0.000 description 1
102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 1
KJCVRFUGPWSIIH-UHFFFAOYSA-N 1-naphthol Chemical compound C1=CC=C2C(O)=CC=CC2=C1 KJCVRFUGPWSIIH-UHFFFAOYSA-N 0.000 description 1
101150072734 1a gene Proteins 0.000 description 1
VGKZZKKTZKRCPA-UHFFFAOYSA-N 2,3-dichloropyridine-4-carboxylic acid Chemical class OC(=O)C1=CC=NC(Cl)=C1Cl VGKZZKKTZKRCPA-UHFFFAOYSA-N 0.000 description 1
UPUWMQZUXFAUCJ-UHFFFAOYSA-N 2,5-dihydro-1,2-thiazole Chemical group C1SNC=C1 UPUWMQZUXFAUCJ-UHFFFAOYSA-N 0.000 description 1
SXGZJKUKBWWHRA-UHFFFAOYSA-N 2-(N-morpholiniumyl)ethanesulfonate Chemical compound [O-]S(=O)(=O)CC[NH+]1CCOCC1 SXGZJKUKBWWHRA-UHFFFAOYSA-N 0.000 description 1
LMSDCGXQALIMLM-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;iron Chemical compound [Fe].OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O LMSDCGXQALIMLM-UHFFFAOYSA-N 0.000 description 1
OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 1
IJNVARQSRMTMKS-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;dihydrochloride Chemical compound Cl.Cl.OCC(N)(CO)CO IJNVARQSRMTMKS-UHFFFAOYSA-N 0.000 description 1
JLIDBLDQVAYHNE-LXGGSRJLSA-N 2-cis-abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\C1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-LXGGSRJLSA-N 0.000 description 1
239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 description 1
108020005065 3' Flanking Region Proteins 0.000 description 1
XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical compound OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 description 1
WUBBRNOQWQTFEX-UHFFFAOYSA-N 4-aminosalicylic acid Chemical compound NC1=CC=C(C(O)=O)C(O)=C1 WUBBRNOQWQTFEX-UHFFFAOYSA-N 0.000 description 1
KFDVPJUYSDEJTH-UHFFFAOYSA-N 4-ethenylpyridine Chemical compound C=CC1=CC=NC=C1 KFDVPJUYSDEJTH-UHFFFAOYSA-N 0.000 description 1
101150096316 5 gene Proteins 0.000 description 1
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
SITWEMZOJNKJCH-WDSKDSINSA-N Ala-Arg Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)CCCN=C(N)N SITWEMZOJNKJCH-WDSKDSINSA-N 0.000 description 1
QXRNAOYBCYVZCD-BQBZGAKWSA-N Ala-Lys Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)CCCCN QXRNAOYBCYVZCD-BQBZGAKWSA-N 0.000 description 1
LIWMQSWFLXEGMA-WDSKDSINSA-N Ala-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](C)N LIWMQSWFLXEGMA-WDSKDSINSA-N 0.000 description 1
102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
244000291564 Allium cepa Species 0.000 description 1
235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
FDQGNLOWMMVRQL-UHFFFAOYSA-N Allobarbital Chemical compound C=CCC1(CC=C)C(=O)NC(=O)NC1=O FDQGNLOWMMVRQL-UHFFFAOYSA-N 0.000 description 1
108090000915 Aminopeptidases Proteins 0.000 description 1
102000004400 Aminopeptidases Human genes 0.000 description 1
ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 1
229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 1
229940122816 Amylase inhibitor Drugs 0.000 description 1
238000006677 Appel reaction Methods 0.000 description 1
241001605719 Appias drusilla Species 0.000 description 1
241000219195 Arabidopsis thaliana Species 0.000 description 1
SJUXYGVRSGTPMC-IMJSIDKUSA-N Asn-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@@H](N)CC(N)=O SJUXYGVRSGTPMC-IMJSIDKUSA-N 0.000 description 1
GFGUPLIETCNQGF-DCAQKATOSA-N Asn-Pro-His Chemical compound C1C[C@H](N(C1)C(=O)[C@H](CC(=O)N)N)C(=O)N[C@@H](CC2=CN=CN2)C(=O)O GFGUPLIETCNQGF-DCAQKATOSA-N 0.000 description 1
JHFNSBBHKSZXKB-VKHMYHEASA-N Asp-Gly Chemical compound OC(=O)C[C@H](N)C(=O)NCC(O)=O JHFNSBBHKSZXKB-VKHMYHEASA-N 0.000 description 1
DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 description 1
101000878902 Bacillus thuringiensis Pesticidal crystal protein Cry6Aa Proteins 0.000 description 1
101000878906 Bacillus thuringiensis Pesticidal crystal protein Cry6Ba Proteins 0.000 description 1
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
DLEJXFOWLRKJEN-UHFFFAOYSA-N C(CCC)N(N)C(=O)C1=CC=CC=2N=NSC21 Chemical compound C(CCC)N(N)C(=O)C1=CC=CC=2N=NSC21 DLEJXFOWLRKJEN-UHFFFAOYSA-N 0.000 description 1
RQWZGSHUMUYSMD-UHFFFAOYSA-N CC(C)(C)N(C(=O)C1=C2C(=CC=C1)N=NS2)N Chemical compound CC(C)(C)N(C(=O)C1=C2C(=CC=C1)N=NS2)N RQWZGSHUMUYSMD-UHFFFAOYSA-N 0.000 description 1
KJBQDYDTQCIAFO-UHFFFAOYSA-N CC(C)CC(N)C(O)=O.NCCCCC(N)C(O)=O Chemical compound CC(C)CC(N)C(O)=O.NCCCCC(N)C(O)=O KJBQDYDTQCIAFO-UHFFFAOYSA-N 0.000 description 1
241000244203 Caenorhabditis elegans Species 0.000 description 1
101100401100 Caenorhabditis elegans mes-1 gene Proteins 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
241000283707 Capra Species 0.000 description 1
239000005745 Captan Substances 0.000 description 1
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
235000019743 Choline chloride Nutrition 0.000 description 1
102100034229 Citramalyl-CoA lyase, mitochondrial Human genes 0.000 description 1
108020004705 Codon Proteins 0.000 description 1
208000035473 Communicable disease Diseases 0.000 description 1
108020004635 Complementary DNA Proteins 0.000 description 1
235000009849 Cucumis sativus Nutrition 0.000 description 1
KOHBWQDSVCARMI-BWBBJGPYSA-N Cys-Cys-Thr Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H](CS)C(=O)N[C@@H]([C@@H](C)O)C(O)=O KOHBWQDSVCARMI-BWBBJGPYSA-N 0.000 description 1
YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
108020001019 DNA Primers Proteins 0.000 description 1
238000007399 DNA isolation Methods 0.000 description 1
239000003155 DNA primer Substances 0.000 description 1
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
241000483370 Dactylia Species 0.000 description 1
241000630627 Diodella Species 0.000 description 1
241001057636 Dracaena deremensis Species 0.000 description 1
101100533511 Drosophila melanogaster SIFaR gene Proteins 0.000 description 1
108700034853 E coli TRPR Proteins 0.000 description 1
108010042407 Endonucleases Proteins 0.000 description 1
208000007984 Female Infertility Diseases 0.000 description 1
229920001917 Ficoll Polymers 0.000 description 1
229930091371 Fructose Natural products 0.000 description 1
239000005715 Fructose Substances 0.000 description 1
229920001503 Glucan Polymers 0.000 description 1
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
102000005720 Glutathione transferase Human genes 0.000 description 1
108010070675 Glutathione transferase Proteins 0.000 description 1
239000004471 Glycine Substances 0.000 description 1
239000005562 Glyphosate Substances 0.000 description 1
101150031823 HSP70 gene Proteins 0.000 description 1
102000002812 Heat-Shock Proteins Human genes 0.000 description 1
KRBMQYPTDYSENE-BQBZGAKWSA-N His-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CNC=N1 KRBMQYPTDYSENE-BQBZGAKWSA-N 0.000 description 1
UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
102100024319 Intestinal-type alkaline phosphatase Human genes 0.000 description 1
101710184243 Intestinal-type alkaline phosphatase Proteins 0.000 description 1
108091092195 Intron Proteins 0.000 description 1
108010044467 Isoenzymes Proteins 0.000 description 1
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
SITWEMZOJNKJCH-UHFFFAOYSA-N L-alanine-L-arginine Natural products CC(N)C(=O)NC(C(O)=O)CCCNC(N)=N SITWEMZOJNKJCH-UHFFFAOYSA-N 0.000 description 1
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 1
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
QOOWRKBDDXQRHC-BQBZGAKWSA-N L-lysyl-L-alanine Chemical compound OC(=O)[C@H](C)NC(=O)[C@@H](N)CCCCN QOOWRKBDDXQRHC-BQBZGAKWSA-N 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
229930182821 L-proline Natural products 0.000 description 1
KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
GDBQQVLCIARPGH-UHFFFAOYSA-N Leupeptin Natural products CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(C=O)CCCN=C(N)N GDBQQVLCIARPGH-UHFFFAOYSA-N 0.000 description 1
241000209510 Liliopsida Species 0.000 description 1
108020004687 Malate Synthase Proteins 0.000 description 1
235000010624 Medicago sativa Nutrition 0.000 description 1
241000721621 Myzus persicae Species 0.000 description 1
OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 1
101150005851 NOS gene Proteins 0.000 description 1
241000244206 Nematoda Species 0.000 description 1
229930193140 Neomycin Natural products 0.000 description 1
101100109871 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) aro-8 gene Proteins 0.000 description 1
101001068537 Nicotiana tabacum Pathogenesis-related protein 1A Proteins 0.000 description 1
239000004677 Nylon Substances 0.000 description 1
102000043276 Oncogene Human genes 0.000 description 1
108700020796 Oncogene Proteins 0.000 description 1
108700026244 Open Reading Frames Proteins 0.000 description 1
241000283973 Oryctolagus cuniculus Species 0.000 description 1
238000012408 PCR amplification Methods 0.000 description 1
239000008118 PEG 6000 Substances 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
101710096342 Pathogenesis-related protein Proteins 0.000 description 1
108010029182 Pectin lyase Proteins 0.000 description 1
108091005804 Peptidases Proteins 0.000 description 1
102000035195 Peptidases Human genes 0.000 description 1
OAICVXFJPJFONN-OUBTZVSYSA-N Phosphorus-32 Chemical compound [32P] OAICVXFJPJFONN-OUBTZVSYSA-N 0.000 description 1
108091000080 Phosphotransferase Proteins 0.000 description 1
241000233629 Phytophthora parasitica Species 0.000 description 1
239000005595 Picloram Substances 0.000 description 1
239000004698 Polyethylene Substances 0.000 description 1
229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
101710117018 Prolactin receptor Proteins 0.000 description 1
ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
239000004365 Protease Substances 0.000 description 1
102100024601 Protein tyrosine phosphatase type IVA 3 Human genes 0.000 description 1
241000589516 Pseudomonas Species 0.000 description 1
241000589624 Pseudomonas amygdali pv. tabaci Species 0.000 description 1
108090000919 Pyroglutamyl-Peptidase I Proteins 0.000 description 1
238000002123 RNA extraction Methods 0.000 description 1
239000013614 RNA sample Substances 0.000 description 1
AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
235000003846 Ricinus Nutrition 0.000 description 1
241000322381 Ricinus <louse> Species 0.000 description 1
241000209056 Secale Species 0.000 description 1
235000007238 Secale cereale Nutrition 0.000 description 1
WBAXJMCUFIXCNI-WDSKDSINSA-N Ser-Pro Chemical compound OC[C@H](N)C(=O)N1CCC[C@H]1C(O)=O WBAXJMCUFIXCNI-WDSKDSINSA-N 0.000 description 1
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
108010071390 Serum Albumin Proteins 0.000 description 1
102000007562 Serum Albumin Human genes 0.000 description 1
DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 description 1
239000005708 Sodium hypochlorite Substances 0.000 description 1
101000966595 Solanum lycopersicum Glucan endo-1,3-beta-glucosidase B Proteins 0.000 description 1
244000061457 Solanum nigrum Species 0.000 description 1
235000002594 Solanum nigrum Nutrition 0.000 description 1
235000011684 Sorghum saccharatum Nutrition 0.000 description 1
244000062793 Sorghum vulgare Species 0.000 description 1
238000002105 Southern blotting Methods 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
101000720079 Stichodactyla helianthus DELTA-stichotoxin-She4a Proteins 0.000 description 1
NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
239000008051 TBE buffer Substances 0.000 description 1
239000004098 Tetracycline Substances 0.000 description 1
AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
239000004473 Threonine Substances 0.000 description 1
241000219793 Trifolium Species 0.000 description 1
244000042324 Trifolium repens Species 0.000 description 1
235000013540 Trifolium repens var repens Nutrition 0.000 description 1
241000209140 Triticum Species 0.000 description 1
235000021307 Triticum Nutrition 0.000 description 1
240000000581 Triticum monococcum Species 0.000 description 1
235000007251 Triticum monococcum Nutrition 0.000 description 1
MYVYPSWUSKCCHG-JQWIXIFHSA-N Trp-Ser Chemical compound C1=CC=C2C(C[C@H](N)C(=O)N[C@@H](CO)C(O)=O)=CNC2=C1 MYVYPSWUSKCCHG-JQWIXIFHSA-N 0.000 description 1
JHDZONWZTCKTJR-KJEVXHAQSA-N Tyr-Thr-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 JHDZONWZTCKTJR-KJEVXHAQSA-N 0.000 description 1
241000726445 Viroids Species 0.000 description 1
229930003779 Vitamin B12 Natural products 0.000 description 1
208000027418 Wounds and injury Diseases 0.000 description 1
SRBFZHDQGSBBOR-IOVATXLUSA-N Xylose Natural products O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 1
241000482268 Zea mays subsp. mays Species 0.000 description 1
CTCBPRXHVPZNHB-VQFZJOCSSA-N [[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate;(2r,3r,4s,5r)-2-(6-aminopurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O.C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O CTCBPRXHVPZNHB-VQFZJOCSSA-N 0.000 description 1
230000002159 abnormal effect Effects 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
239000008351 acetate buffer Substances 0.000 description 1
229960001138 acetylsalicylic acid Drugs 0.000 description 1
230000003044 adaptive effect Effects 0.000 description 1
229960000643 adenine Drugs 0.000 description 1
239000003463 adsorbent Substances 0.000 description 1
108010045649 agarase Proteins 0.000 description 1
235000004279 alanine Nutrition 0.000 description 1
125000001931 aliphatic group Chemical group 0.000 description 1
125000003342 alkenyl group Chemical group 0.000 description 1
125000004183 alkoxy alkyl group Chemical group 0.000 description 1
125000005081 alkoxyalkoxyalkyl group Chemical group 0.000 description 1
125000000304 alkynyl group Chemical group 0.000 description 1
102000004139 alpha-Amylases Human genes 0.000 description 1
108090000637 alpha-Amylases Proteins 0.000 description 1
229940024171 alpha-amylase Drugs 0.000 description 1
125000000539 amino acid group Chemical group 0.000 description 1
229960004050 aminobenzoic acid Drugs 0.000 description 1
235000012538 ammonium bicarbonate Nutrition 0.000 description 1
239000001099 ammonium carbonate Substances 0.000 description 1
239000001166 ammonium sulphate Substances 0.000 description 1
230000003321 amplification Effects 0.000 description 1
239000003392 amylase inhibitor Substances 0.000 description 1
229940025131 amylases Drugs 0.000 description 1
ALYNCZNDIQEVRV-UHFFFAOYSA-N aniline-p-carboxylic acid Natural products NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 1
210000004102 animal cell Anatomy 0.000 description 1
238000005571 anion exchange chromatography Methods 0.000 description 1
150000001450 anions Chemical class 0.000 description 1
238000000137 annealing Methods 0.000 description 1
239000000427 antigen Substances 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
239000004599 antimicrobial Substances 0.000 description 1
PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
230000001174 ascending effect Effects 0.000 description 1
235000009582 asparagine Nutrition 0.000 description 1
229960001230 asparagine Drugs 0.000 description 1
235000003704 aspartic acid Nutrition 0.000 description 1
108010047857 aspartylglycine Proteins 0.000 description 1
239000012131 assay buffer Substances 0.000 description 1
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
238000000211 autoradiogram Methods 0.000 description 1
PZYASAHPHYCDDC-UHFFFAOYSA-N benzyl 1,2,3-benzothiadiazole-7-carboxylate Chemical compound C=1C=CC=2N=NSC=2C=1C(=O)OCC1=CC=CC=C1 PZYASAHPHYCDDC-UHFFFAOYSA-N 0.000 description 1
INXDXESZDNREDU-UHFFFAOYSA-N benzyl 2-chloro-5-methyl-4-(trifluoromethyl)-3h-1,3-thiazole-2-carboxylate Chemical compound S1C(C)=C(C(F)(F)F)NC1(Cl)C(=O)OCC1=CC=CC=C1 INXDXESZDNREDU-UHFFFAOYSA-N 0.000 description 1
SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
239000003139 biocide Substances 0.000 description 1
230000005540 biological transmission Effects 0.000 description 1
229960002685 biotin Drugs 0.000 description 1
235000020958 biotin Nutrition 0.000 description 1
239000011616 biotin Substances 0.000 description 1
230000036983 biotransformation Effects 0.000 description 1
230000000903 blocking effect Effects 0.000 description 1
238000009395 breeding Methods 0.000 description 1
230000001488 breeding effect Effects 0.000 description 1
AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
239000001110 calcium chloride Substances 0.000 description 1
229910001628 calcium chloride Inorganic materials 0.000 description 1
229940117949 captan Drugs 0.000 description 1
229910052799 carbon Inorganic materials 0.000 description 1
125000004432 carbon atom Chemical group C* 0.000 description 1
230000034303 cell budding Effects 0.000 description 1
210000003855 cell nucleus Anatomy 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
230000008859 change Effects 0.000 description 1
235000013351 cheese Nutrition 0.000 description 1
108091000085 chlorophyll binding Proteins 0.000 description 1
229960003178 choline chloride Drugs 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
239000013611 chromosomal DNA Substances 0.000 description 1
235000015165 citric acid Nutrition 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
230000000536 complexating effect Effects 0.000 description 1
238000010924 continuous production Methods 0.000 description 1
238000001816 cooling Methods 0.000 description 1
230000002596 correlated effect Effects 0.000 description 1
235000012343 cottonseed oil Nutrition 0.000 description 1
235000020247 cow milk Nutrition 0.000 description 1
239000012228 culture supernatant Substances 0.000 description 1
ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
125000001316 cycloalkyl alkyl group Chemical group 0.000 description 1
125000000753 cycloalkyl group Chemical group 0.000 description 1
235000018417 cysteine Nutrition 0.000 description 1
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
229960003067 cystine Drugs 0.000 description 1
229940104302 cytosine Drugs 0.000 description 1
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
230000007423 decrease Effects 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
230000007123 defense Effects 0.000 description 1
238000001514 detection method Methods 0.000 description 1
238000001784 detoxification Methods 0.000 description 1
230000001627 detrimental effect Effects 0.000 description 1
238000010586 diagram Methods 0.000 description 1
235000013681 dietary sucrose Nutrition 0.000 description 1
ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
239000002270 dispersing agent Substances 0.000 description 1
239000006185 dispersion Substances 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
230000003828 downregulation Effects 0.000 description 1
239000003814 drug Substances 0.000 description 1
238000001035 drying Methods 0.000 description 1
239000000428 dust Substances 0.000 description 1
230000013020 embryo development Effects 0.000 description 1
239000003995 emulsifying agent Substances 0.000 description 1
230000001804 emulsifying effect Effects 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
238000001976 enzyme digestion Methods 0.000 description 1
150000002148 esters Chemical class 0.000 description 1
238000005530 etching Methods 0.000 description 1
210000003527 eukaryotic cell Anatomy 0.000 description 1
238000010195 expression analysis Methods 0.000 description 1
230000002349 favourable effect Effects 0.000 description 1
230000004720 fertilization Effects 0.000 description 1
239000003337 fertilizer Substances 0.000 description 1
230000004883 flower formation Effects 0.000 description 1
239000003269 fluorescent indicator Substances 0.000 description 1
239000011888 foil Substances 0.000 description 1
229960000304 folic acid Drugs 0.000 description 1
235000019152 folic acid Nutrition 0.000 description 1
239000011724 folic acid Substances 0.000 description 1
230000037406 food intake Effects 0.000 description 1
239000004459 forage Substances 0.000 description 1
239000003205 fragrance Substances 0.000 description 1
238000007710 freezing Methods 0.000 description 1
230000008014 freezing Effects 0.000 description 1
235000011087 fumaric acid Nutrition 0.000 description 1
230000002538 fungal effect Effects 0.000 description 1
230000000855 fungicidal effect Effects 0.000 description 1
239000007789 gas Substances 0.000 description 1
239000003349 gelling agent Substances 0.000 description 1
230000035784 germination Effects 0.000 description 1
239000012869 germination medium Substances 0.000 description 1
IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
239000011521 glass Substances 0.000 description 1
235000013922 glutamic acid Nutrition 0.000 description 1
239000004220 glutamic acid Substances 0.000 description 1
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
125000000404 glutamine group Chemical class N[C@@H](CCC(N)=O)C(=O)* 0.000 description 1
XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
229940097068 glyphosate Drugs 0.000 description 1
239000008187 granular material Substances 0.000 description 1
235000021384 green leafy vegetables Nutrition 0.000 description 1
230000009036 growth inhibition Effects 0.000 description 1
229960000789 guanidine hydrochloride Drugs 0.000 description 1
PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
125000005843 halogen group Chemical group 0.000 description 1
238000003306 harvesting Methods 0.000 description 1
239000011874 heated mixture Substances 0.000 description 1
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
108010018006 histidylserine Proteins 0.000 description 1
238000000265 homogenisation Methods 0.000 description 1
229940088597 hormone Drugs 0.000 description 1
239000005556 hormone Substances 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
238000007654 immersion Methods 0.000 description 1
238000001114 immunoprecipitation Methods 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
208000000509 infertility Diseases 0.000 description 1
208000021267 infertility disease Diseases 0.000 description 1
239000003112 inhibitor Substances 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
208000014674 injury Diseases 0.000 description 1
238000011081 inoculation Methods 0.000 description 1
229960000367 inositol Drugs 0.000 description 1
230000000749 insecticidal effect Effects 0.000 description 1
238000007689 inspection Methods 0.000 description 1
229960000310 isoleucine Drugs 0.000 description 1
AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
TWBYWOBDOCUKOW-UHFFFAOYSA-N isonicotinic acid Chemical group OC(=O)C1=CC=NC=C1 TWBYWOBDOCUKOW-UHFFFAOYSA-N 0.000 description 1
150000002576 ketones Chemical class 0.000 description 1
GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 1
108010052968 leupeptin Proteins 0.000 description 1
239000012669 liquid formulation Substances 0.000 description 1
239000012160 loading buffer Substances 0.000 description 1
235000011090 malic acid Nutrition 0.000 description 1
230000013011 mating Effects 0.000 description 1
230000035800 maturation Effects 0.000 description 1
230000010534 mechanism of action Effects 0.000 description 1
230000008018 melting Effects 0.000 description 1
230000002503 metabolic effect Effects 0.000 description 1
229910052751 metal Inorganic materials 0.000 description 1
239000002184 metal Substances 0.000 description 1
MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
UZKWTJUDCOPSNM-UHFFFAOYSA-N methoxybenzene Substances CCCCOC=C UZKWTJUDCOPSNM-UHFFFAOYSA-N 0.000 description 1
OSWPMRLSEDHDFF-UHFFFAOYSA-N methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 description 1
244000005700 microbiome Species 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
239000006870 ms-medium Substances 0.000 description 1
230000036438 mutation frequency Effects 0.000 description 1
125000001326 naphthylalkyl group Chemical group 0.000 description 1
229930014626 natural product Natural products 0.000 description 1
235000021096 natural sweeteners Nutrition 0.000 description 1
229960004927 neomycin Drugs 0.000 description 1
235000005152 nicotinamide Nutrition 0.000 description 1
239000011570 nicotinamide Substances 0.000 description 1
238000010899 nucleation Methods 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
210000004940 nucleus Anatomy 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
235000016709 nutrition Nutrition 0.000 description 1
229920001778 nylon Polymers 0.000 description 1
150000002894 organic compounds Chemical class 0.000 description 1
230000003204 osmotic effect Effects 0.000 description 1
239000001301 oxygen Substances 0.000 description 1
238000004806 packaging method and process Methods 0.000 description 1
230000007170 pathology Effects 0.000 description 1
101150072645 pea gene Proteins 0.000 description 1
239000003415 peat Substances 0.000 description 1
239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
230000000737 periodic effect Effects 0.000 description 1
125000005359 phenoxyalkyl group Chemical group 0.000 description 1
COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
125000003884 phenylalkyl group Chemical group 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
229910052698 phosphorus Inorganic materials 0.000 description 1
239000011574 phosphorus Substances 0.000 description 1
102000020233 phosphotransferase Human genes 0.000 description 1
231100000208 phytotoxic Toxicity 0.000 description 1
230000000885 phytotoxic effect Effects 0.000 description 1
NQQVFXUMIDALNH-UHFFFAOYSA-N picloram Chemical compound NC1=C(Cl)C(Cl)=NC(C(O)=O)=C1Cl NQQVFXUMIDALNH-UHFFFAOYSA-N 0.000 description 1
SIOXPEMLGUPBBT-UHFFFAOYSA-N picolinic acid Chemical group OC(=O)C1=CC=CC=N1 SIOXPEMLGUPBBT-UHFFFAOYSA-N 0.000 description 1
239000000419 plant extract Substances 0.000 description 1
238000004161 plant tissue culture Methods 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920000573 polyethylene Polymers 0.000 description 1
238000003752 polymerase chain reaction Methods 0.000 description 1
229920000136 polysorbate Polymers 0.000 description 1
239000011148 porous material Substances 0.000 description 1
235000012015 potatoes Nutrition 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
239000002243 precursor Substances 0.000 description 1
230000002035 prolonged effect Effects 0.000 description 1
LGGNBHZZNNEZCQ-UHFFFAOYSA-N propyl 1,2,3-benzothiadiazole-7-carboxylate Chemical compound CCCOC(=O)C1=CC=CC2=C1SN=N2 LGGNBHZZNNEZCQ-UHFFFAOYSA-N 0.000 description 1
239000003223 protective agent Substances 0.000 description 1
238000011002 quantification Methods 0.000 description 1
238000004445 quantitative analysis Methods 0.000 description 1
239000000700 radioactive tracer Substances 0.000 description 1
239000002994 raw material Substances 0.000 description 1
239000012429 reaction media Substances 0.000 description 1
230000008844 regulatory mechanism Effects 0.000 description 1
239000011347 resin Substances 0.000 description 1
229920005989 resin Polymers 0.000 description 1
108010074709 ronidase Proteins 0.000 description 1
230000007017 scission Effects 0.000 description 1
CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
239000013049 sediment Substances 0.000 description 1
230000010153 self-pollination Effects 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
108010026333 seryl-proline Proteins 0.000 description 1
150000004760 silicates Chemical class 0.000 description 1
238000010583 slow cooling Methods 0.000 description 1
239000002002 slurry Substances 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
MKWYFZFMAMBPQK-UHFFFAOYSA-J sodium feredetate Chemical compound [Na+].[Fe+3].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O MKWYFZFMAMBPQK-UHFFFAOYSA-J 0.000 description 1
235000010267 sodium hydrogen sulphite Nutrition 0.000 description 1
210000001082 somatic cell Anatomy 0.000 description 1
238000009331 sowing Methods 0.000 description 1
239000008107 starch Substances 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
238000003756 stirring Methods 0.000 description 1
238000003860 storage Methods 0.000 description 1
229960005322 streptomycin Drugs 0.000 description 1
150000008163 sugars Chemical class 0.000 description 1
FRGKKTITADJNOE-UHFFFAOYSA-N sulfanyloxyethane Chemical compound CCOS FRGKKTITADJNOE-UHFFFAOYSA-N 0.000 description 1
239000011593 sulfur Substances 0.000 description 1
235000020238 sunflower seed Nutrition 0.000 description 1
230000001629 suppression Effects 0.000 description 1
239000004094 surface-active agent Substances 0.000 description 1
238000004114 suspension culture Methods 0.000 description 1
230000002459 sustained effect Effects 0.000 description 1
230000001360 synchronised effect Effects 0.000 description 1
230000002194 synthesizing effect Effects 0.000 description 1
230000009885 systemic effect Effects 0.000 description 1
229960002180 tetracycline Drugs 0.000 description 1
229930101283 tetracycline Natural products 0.000 description 1
235000019364 tetracycline Nutrition 0.000 description 1
150000003522 tetracyclines Chemical class 0.000 description 1
229960003495 thiamine Drugs 0.000 description 1
DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
235000019190 thiamine hydrochloride Nutrition 0.000 description 1
239000011747 thiamine hydrochloride Substances 0.000 description 1
229960002363 thiamine pyrophosphate Drugs 0.000 description 1
235000008170 thiamine pyrophosphate Nutrition 0.000 description 1
239000011678 thiamine pyrophosphate Substances 0.000 description 1
YXVCLPJQTZXJLH-UHFFFAOYSA-N thiamine(1+) diphosphate chloride Chemical compound [Cl-].CC1=C(CCOP(O)(=O)OP(O)(O)=O)SC=[N+]1CC1=CN=C(C)N=C1N YXVCLPJQTZXJLH-UHFFFAOYSA-N 0.000 description 1
150000003566 thiocarboxylic acids Chemical class 0.000 description 1
229940104230 thymidine Drugs 0.000 description 1
230000009772 tissue formation Effects 0.000 description 1
108700012359 toxins Proteins 0.000 description 1
230000005026 transcription initiation Effects 0.000 description 1
238000000844 transformation Methods 0.000 description 1
230000010474 transient expression Effects 0.000 description 1
UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
125000000430 tryptophan group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12 0.000 description 1
108010045269 tryptophyltryptophan Proteins 0.000 description 1
WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
229910052721 tungsten Inorganic materials 0.000 description 1
239000010937 tungsten Substances 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
230000003827 upregulation Effects 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
229940035893 uracil Drugs 0.000 description 1
239000004474 valine Substances 0.000 description 1
230000003253 viricidal effect Effects 0.000 description 1
230000001018 virulence Effects 0.000 description 1
238000012800 visualization Methods 0.000 description 1
235000019163 vitamin B12 Nutrition 0.000 description 1
239000011715 vitamin B12 Substances 0.000 description 1
230000003442 weekly effect Effects 0.000 description 1
238000009736 wetting Methods 0.000 description 1
239000000080 wetting agent Substances 0.000 description 1
NLIVDORGVGAOOJ-MAHBNPEESA-M xylene cyanol Chemical compound [Na+].C1=C(C)C(NCC)=CC=C1C(\C=1C(=CC(OS([O-])=O)=CC=1)OS([O-])=O)=C\1C=C(C)\C(=[NH+]/CC)\C=C/1 NLIVDORGVGAOOJ-MAHBNPEESA-M 0.000 description 1
235000020138 yakult Nutrition 0.000 description 1

Classifications

- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

PL89278117A 1988-03-08 1989-03-07 Method for controlling transcription of a chemically controlled gene in plant material PL162317B1 (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US16566788A	1988-03-08	1988-03-08

Publications (2)

Publication Number	Publication Date
PL278117A1 PL278117A1 (en)	1989-11-27
PL162317B1 true PL162317B1 (en)	1993-09-30

Family

ID=22599926

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
PL89278117A PL162317B1 (en)	1988-03-08	1989-03-07	Method for controlling transcription of a chemically controlled gene in plant material

Country Status (3)

Country	Link
DD (1)	DD283647A5 (de)
PL (1)	PL162317B1 (de)
ZA (1)	ZA891726B (de)

1989
- 1989-03-06 DD DD89326307A patent/DD283647A5/de not_active IP Right Cessation
- 1989-03-07 PL PL89278117A patent/PL162317B1/pl unknown
- 1989-03-07 ZA ZA891726A patent/ZA891726B/xx unknown

Also Published As

Publication number	Publication date
PL278117A1 (en)	1989-11-27
ZA891726B (en)	1989-11-29
DD283647A5 (de)	1990-10-17

Publication	Publication Date	Title
AU617433B2 (en)	1991-11-28	Chemically regulatable dna sequences and genes and uses thereof
US5847258A (en)	1998-12-08	DNA encoding β-1,3-glucanases
US5789214A (en)	1998-08-04	Method of inducing gene transcription in a plant
CA2012778C (en)	2005-08-09	Disease-resistant transgenic plants
US6107549A (en)	2000-08-22	Genetically engineered plant resistance to thiazopyr and other pyridine herbicides
US5571706A (en)	1996-11-05	Plant virus resistance gene and methods
AU610825B2 (en)	1991-05-30	Herbicide tolerant plants containing gluthathione S-transferase gene
HUP0201018A2 (en)	2002-07-29	Herbicide resistant plants
JPH10507913A (ja)	1998-08-04	ジャガイモ由来のプロモーター配列
US6054637A (en)	2000-04-25	Signal sequences for vacuolar sorting
US7767801B2 (en)	2010-08-03	Tissue specific promoters
BRPI1011495B1 (pt)	2019-05-14	Método para produção de uma planta transgênica tendo tolerância aumentada ao estresse por calor em relação a um controle do tipo selvagem
AU719467B2 (en)	2000-05-11	Method for regulating cell death
US5276269A (en)	1994-01-04	Lectin cDNA and transgenic plants derived therefrom
US5851766A (en)	1998-12-22	Process for isolating chemically regulatable DNA sequences
PL162317B1 (en)	1993-09-30	Method for controlling transcription of a chemically controlled gene in plant material
US6653463B1 (en)	2003-11-25	Biocidal protein
CN100424177C (zh)	2008-10-08	融合杀虫基因cryci及其应用
US7094952B1 (en)	2006-08-22	Method for obtaining transgenic plants expressing a protein with activity producing hydrogen peroxide by transformation by agrobacterium rhizogenes
US20020069431A1 (en)	2002-06-06	Effect of endochitinase and chitobiosidase and their encoding genes on plant growth and development