PL354444A1 - Sekwencje sygnałowe do wytwarzania leu-hirudyny przez wydzielanie do pożywki hodowlanej przez E.coli - Google Patents

Sekwencje sygnałowe do wytwarzania leu-hirudyny przez wydzielanie do pożywki hodowlanej przez E.coli

Info

Publication number
PL354444A1
PL354444A1 PL00354444A PL35444400A PL354444A1 PL 354444 A1 PL354444 A1 PL 354444A1 PL 00354444 A PL00354444 A PL 00354444A PL 35444400 A PL35444400 A PL 35444400A PL 354444 A1 PL354444 A1 PL 354444A1
Authority
PL
Poland
Prior art keywords
hirudine
leu
coli
production
signal sequences
Prior art date
Application number
PL00354444A
Other languages
English (en)
Other versions
PL205117B1 (pl
Inventor
Paul Habermann
Rudolf Bender
Original Assignee
Aventis Pharma Deutschland Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Deutschland Gmbh filed Critical Aventis Pharma Deutschland Gmbh
Publication of PL354444A1 publication Critical patent/PL354444A1/pl
Publication of PL205117B1 publication Critical patent/PL205117B1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PL354444A 1999-09-18 2000-09-01 Prekursor hirudyny obejmujący sekwencję sygnałową, jego zastosowanie oraz sposób wytwarzania Leu-hirudyny PL205117B1 (pl)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19944870A DE19944870A1 (de) 1999-09-18 1999-09-18 Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
PCT/EP2000/008537 WO2001021662A1 (de) 1999-09-18 2000-09-01 Signalsequenzen zur herstellung von leu-hirudin über sekretion durch e. coli in das kulturmedium

Publications (2)

Publication Number Publication Date
PL354444A1 true PL354444A1 (pl) 2004-01-12
PL205117B1 PL205117B1 (pl) 2010-03-31

Family

ID=7922547

Family Applications (1)

Application Number Title Priority Date Filing Date
PL354444A PL205117B1 (pl) 1999-09-18 2000-09-01 Prekursor hirudyny obejmujący sekwencję sygnałową, jego zastosowanie oraz sposób wytwarzania Leu-hirudyny

Country Status (28)

Country Link
US (1) US7455987B1 (pl)
EP (1) EP1216259B1 (pl)
JP (1) JP4669181B2 (pl)
KR (1) KR100737189B1 (pl)
CN (2) CN1269838C (pl)
AR (1) AR025660A1 (pl)
AT (1) ATE357458T1 (pl)
AU (1) AU775923B2 (pl)
BR (1) BR0014519A (pl)
CA (1) CA2385287C (pl)
CZ (1) CZ2002899A3 (pl)
DE (2) DE19944870A1 (pl)
DK (1) DK1216259T3 (pl)
EE (1) EE05187B1 (pl)
HR (1) HRP20020226B1 (pl)
HU (1) HUP0202596A3 (pl)
IL (2) IL148624A0 (pl)
ME (1) MEP27908A (pl)
MX (1) MXPA02002469A (pl)
NO (1) NO329723B1 (pl)
NZ (1) NZ517825A (pl)
PL (1) PL205117B1 (pl)
RS (1) RS50435B (pl)
RU (1) RU2261867C2 (pl)
SK (1) SK287865B6 (pl)
TR (2) TR200200667T2 (pl)
WO (1) WO2001021662A1 (pl)
ZA (1) ZA200202106B (pl)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7638618B2 (en) 2001-02-20 2009-12-29 Sanofi-Aventis Deutschland Gmbh Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
BRPI0416807A (pt) 2003-11-21 2007-01-09 Dow Global Technologies Inc sistemas de expressão aperfeiçoados com secreção de sistema sec
CN101031655A (zh) 2004-07-26 2007-09-05 陶氏环球技术公司 通过株工程改进蛋白表达的方法
DE102005046113A1 (de) * 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen
DE102006044841A1 (de) 2006-09-22 2008-04-03 Wacker Chemie Ag Signalpeptid zur Produktion von rekombinanten Proteinen
KR101491867B1 (ko) 2007-01-31 2015-02-10 피페넥스 인크. 증가된 발현을 위한 박테리아 리더 서열
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US11084860B2 (en) 2013-03-12 2021-08-10 The General Hospital Corporation Modified Mullerian inhibiting substance (MIS) proteins and uses thereof for the treatment of diseases
US11135269B2 (en) 2013-12-11 2021-10-05 The General Hospital Corporation Use of mullerian inhibiting substance (MIS) proteins for contraception and ovarian reserve preservation
CN108220369B (zh) * 2017-12-04 2021-03-12 广东双骏生物科技有限公司 一种生产重组水蛭素的方法
CN108359689B (zh) * 2018-01-19 2021-03-23 华中农业大学 利用抗病基因识别无毒效应蛋白以鉴定具有分泌功能信号肽的方法
CN115572329B (zh) * 2021-06-21 2024-02-06 王大勇 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE58903988D1 (de) * 1985-04-11 1993-05-13 Hoechst Ag Hirudin-derivat.
US5084384A (en) * 1987-04-23 1992-01-28 Monsanto Company Secretion of insulin-like growth factor-I
AU604925B2 (en) * 1988-02-23 1991-01-03 Schering Aktiengesellschaft A hirudin derivative
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
DE69130872T2 (de) * 1990-11-08 1999-08-26 Japan Energy Corp. Sekretionsvektor, diesen enthaltene transformierte Mikroorganismen und Herstellung von Produkten durch obigen Mikroorganismus
JPH07119237B2 (ja) * 1990-11-08 1995-12-20 株式会社ジャパンエナジー ヒルジン変異体，その製造法及び抗凝血剤
PT100580A (pt) * 1991-06-12 1993-09-30 Regeneron Pharma Producao e recuperacao de neurotrofinas recombinantes
US5389529A (en) * 1991-06-12 1995-02-14 Regeneron Pharmaceuticals, Inc. Modified lamβ signal sequence and processes for producing recombinant neurotrophins
DE4140381A1 (de) 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De Neue synthetische isohirudine mit verbesserter stabilitaet

Also Published As

Publication number Publication date
TR200200667T2 (tr) 2002-06-21
NO20021254D0 (no) 2002-03-13
AR025660A1 (es) 2002-12-11
HUP0202596A2 (hu) 2002-12-28
RU2261867C2 (ru) 2005-10-10
SK287865B6 (sk) 2012-02-03
ZA200202106B (en) 2003-01-09
HK1049172A1 (en) 2003-05-02
JP2003510041A (ja) 2003-03-18
JP4669181B2 (ja) 2011-04-13
NZ517825A (en) 2004-07-30
IL148624A0 (en) 2002-09-12
CA2385287A1 (en) 2001-03-29
HRP20020226B1 (hr) 2010-08-31
HRP20020226A2 (en) 2004-04-30
HUP0202596A3 (en) 2005-01-28
EP1216259B1 (de) 2007-03-21
TR200501541T2 (tr) 2005-06-21
EP1216259A1 (de) 2002-06-26
PL205117B1 (pl) 2010-03-31
CN1192037C (zh) 2005-03-09
RU2002110278A (ru) 2004-03-27
DK1216259T3 (da) 2007-07-02
CA2385287C (en) 2010-10-19
HK1068352A1 (en) 2005-04-29
RS50435B (sr) 2009-12-31
NO329723B1 (no) 2010-12-06
BR0014519A (pt) 2002-06-11
YU14602A (sh) 2005-07-19
DE19944870A1 (de) 2001-03-29
CN1269838C (zh) 2006-08-16
SK3622002A3 (en) 2003-06-03
KR20020034188A (ko) 2002-05-08
DE50014189D1 (de) 2007-05-03
AU6842100A (en) 2001-04-24
EE200200139A (et) 2003-06-16
CN1374970A (zh) 2002-10-16
KR100737189B1 (ko) 2007-07-10
WO2001021662A1 (de) 2001-03-29
NO20021254L (no) 2002-05-07
CZ2002899A3 (cs) 2002-06-12
IL148624A (en) 2009-08-03
ATE357458T1 (de) 2007-04-15
EE05187B1 (et) 2009-06-15
MXPA02002469A (es) 2002-08-28
CN1550502A (zh) 2004-12-01
MEP27908A (en) 2010-10-10
US7455987B1 (en) 2008-11-25
AU775923B2 (en) 2004-08-19

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Legal Events

Date Code Title Description
LAPS Decisions on the lapse of the protection rights

Effective date: 20120901