RU2017116790A - Инсектицидные белки и способы их применения - Google Patents
Инсектицидные белки и способы их применения Download PDFInfo
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- RU2017116790A RU2017116790A RU2017116790A RU2017116790A RU2017116790A RU 2017116790 A RU2017116790 A RU 2017116790A RU 2017116790 A RU2017116790 A RU 2017116790A RU 2017116790 A RU2017116790 A RU 2017116790A RU 2017116790 A RU2017116790 A RU 2017116790A
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- Prior art keywords
- ptip
- seq
- polynucleotide
- polypeptide
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- 230000000749 insecticidal effect Effects 0.000 title claims 2
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 241000238631 Hexapoda Species 0.000 claims 15
- 108091033319 polynucleotide Proteins 0.000 claims 13
- 102000040430 polynucleotide Human genes 0.000 claims 13
- 239000002157 polynucleotide Substances 0.000 claims 13
- 229920001184 polypeptide Polymers 0.000 claims 12
- 102000004196 processed proteins & peptides Human genes 0.000 claims 12
- 108090000765 processed proteins & peptides Proteins 0.000 claims 12
- 241000196324 Embryophyta Species 0.000 claims 11
- 241000607479 Yersinia pestis Species 0.000 claims 11
- 230000009261 transgenic effect Effects 0.000 claims 5
- 230000001276 controlling effect Effects 0.000 claims 4
- 241000255967 Helicoverpa zea Species 0.000 claims 2
- 241000209149 Zea Species 0.000 claims 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 235000005822 corn Nutrition 0.000 claims 2
- 230000002401 inhibitory effect Effects 0.000 claims 2
- 108020004705 Codon Proteins 0.000 claims 1
- 241000254173 Coleoptera Species 0.000 claims 1
- 241000255777 Lepidoptera Species 0.000 claims 1
- 239000002299 complementary DNA Substances 0.000 claims 1
- 108020001507 fusion proteins Proteins 0.000 claims 1
- 102000037865 fusion proteins Human genes 0.000 claims 1
- 208000015181 infectious disease Diseases 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 239000003053 toxin Substances 0.000 claims 1
- 231100000765 toxin Toxicity 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/04—Pteridophyta [fern allies]; Filicophyta [ferns]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C07K14/08—RNA viruses
- C07K14/145—Rhabdoviridae, e.g. rabies virus, Duvenhage virus, Mokola virus or vesicular stomatitis virus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Insects & Arthropods (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Claims (22)
1. Рекомбинантный полипептид PtIP-96 с последовательностью, по меньшей мере на 80% идентичной аминокислотной последовательности под SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 26, SEQ ID NO: 42 или SEQ ID NO: 54.
2. Рекомбинантный полипептид PtIP-96 по п. 1, где последовательность полипептида PtIP-96 по меньшей мере на 95% идентична аминокислотной последовательности под SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 26, SEQ ID NO: 42 или SEQ ID NO: 54.
3. Рекомбинантный полипептид PtIP-96 по п. 1 или 2, где полипептид PtIP-96 обладает инсектицидной активностью против совки кукурузной (Helicoverpa zea).
4. Рекомбинантный полинуклеотид, кодирующий полипептид PtIP-96 по любому из пп. 1-3.
5. Рекомбинантный полинуклеотид по п. 4, где полинуклеотид представляет собой полинуклеотид, отличающийся от геномного.
6. Рекомбинантный полинуклеотид по п. 5, где полинуклеотид представляет собой кДНК.
7. Рекомбинантный полинуклеотид по п. 5, где полинуклеотид представляет собой синтетический полинуклеотид.
8. Рекомбинантный полинуклеотид по п. 7, где полинуклеотид имеет кодоны, оптимизированные для экспрессии в культуре, важной с точки зрения сельского хозяйства.
9. Трансгенное растение или растительная клетка, содержащие полинуклеотид по любому из пп. 4-8.
10. ДНК-конструкция, содержащая полинуклеотид по любому из пп. 4-8, функционально связанный с гетерологичным регуляторным элементом.
11. Трансгенное растение или растительная клетка, содержащие ДНК-конструкцию по п. 10.
12. Композиция, содержащая полипептид PtIP-96 по любому из пп. 1-3.
13. Белок слияния, содержащий полипептид PtIP-96 по любому из пп. 1-3.
14. Способ контроля популяции насекомого-вредителя, включающий приведение в контакт популяции насекомого-вредителя с полипептидом PtIP-96 по любому из пп. 1-3.
15. Способ подавления роста или уничтожения насекомого-вредителя, включающий приведение в контакт насекомого-вредителя с композицией, содержащей полипептид PtIP-96 по любому из пп. 1-3.
16. Способ контроля заражения трансгенного растения насекомыми из отряда Lepidoptera и/или Coleoptera и обеспечения управления устойчивостью насекомых, включающий экспрессию в растении полипептида PtIP-96 по любому из пп. 1-3.
17. Способ контроля популяции насекомого-вредителя, включающий приведение в контакт популяции насекомого-вредителя с трансгенным растением или растительной клеткой по п. 9 или 11.
18. Способ подавления роста или уничтожения насекомого-вредителя, включающий трансформирование растения ДНК-конструкцией по п. 10.
19. Способ по п. 18, дополнительно включающий приведение в контакт насекомого-вредителя с трансгенным растением или растительной клеткой.
20. Способ по п. 18 или 19, где насекомое-вредитель представляет собой совку кукурузную (Helicoverpa zea).
21. Способ по любому из пп. 15, 16, 17, 18, 19 или 20, где насекомое-вредитель или популяция насекомых-вредителей являются устойчивыми по меньшей мере к одному Bt-токсину.
22. Применение полипептида PtIP-96 по любому из пп. 1-3 для подавления роста или уничтожения насекомого или популяции насекомых.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462064810P | 2014-10-16 | 2014-10-16 | |
| US62/064,810 | 2014-10-16 | ||
| PCT/US2015/055502 WO2016061206A1 (en) | 2014-10-16 | 2015-10-14 | Insecticidal proteins and methods for their use |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| RU2017116790A true RU2017116790A (ru) | 2018-11-19 |
| RU2017116790A3 RU2017116790A3 (ru) | 2019-05-17 |
| RU2733898C2 RU2733898C2 (ru) | 2020-10-09 |
Family
ID=54396965
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2017116790A RU2733898C2 (ru) | 2014-10-16 | 2015-10-14 | Инсектицидные белки и способы их применения |
Country Status (11)
| Country | Link |
|---|---|
| US (5) | US10435706B2 (ru) |
| EP (1) | EP3207143B1 (ru) |
| CN (2) | CN113372421B (ru) |
| AR (1) | AR102293A1 (ru) |
| CA (1) | CA2963558C (ru) |
| MX (1) | MX381370B (ru) |
| PH (1) | PH12017500694A1 (ru) |
| RU (1) | RU2733898C2 (ru) |
| UA (1) | UA126192C2 (ru) |
| WO (1) | WO2016061206A1 (ru) |
| ZA (1) | ZA201702161B (ru) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112016018103B1 (pt) | 2014-02-07 | 2024-01-16 | E.I. Du Pont De Nemours And Company | Polipeptídeo e seu uso, polinucleotídeo, composição, proteína de fusão, método para controlar uma população, método para inibir o crescimento, método para controlar a infestação, método para obtenção de uma planta ou célula vegetal, construto |
| CN113372421B (zh) | 2014-10-16 | 2024-08-06 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| US11213028B2 (en) | 2016-12-22 | 2022-01-04 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| RU2019140646A (ru) | 2017-05-11 | 2021-06-11 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
| BR112020012658A2 (pt) | 2017-12-22 | 2020-12-01 | Pioneer Hi-Bred International, Inc. | construto de dna, planta transgênica, método para controlar uma população de pragas de inseto, polipeptídeo, polinucleotídeo |
| CA3092078A1 (en) | 2018-03-14 | 2019-09-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
| AU2019234562B2 (en) * | 2018-03-14 | 2024-08-01 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
| WO2020046701A1 (en) | 2018-08-29 | 2020-03-05 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| CN112771068A (zh) * | 2018-09-11 | 2021-05-07 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| US20220015372A1 (en) | 2018-12-14 | 2022-01-20 | Pioneer Hi-Bred International, Inc. | Biologicals and their use in plants |
| CN109456976B (zh) * | 2018-12-26 | 2021-09-28 | 菏泽学院 | 舞毒蛾JHAMT基因、其编码蛋白及其dsRNA在害虫防治中的应用 |
| WO2020146439A1 (en) * | 2019-01-10 | 2020-07-16 | Syngenta Crop Protection Ag | Compositions and methods for controlling insect pests |
| EP3708669A1 (en) * | 2019-03-13 | 2020-09-16 | Genective | Pesticidal genes and methods of use |
| WO2021076346A1 (en) | 2019-10-18 | 2021-04-22 | Pioneer Hi-Bred International, Inc. | Maize event dp-202216-6 and dp-023211-2 stack |
| TWI906251B (zh) | 2020-02-04 | 2025-12-01 | 美商科迪華農業科技有限責任公司 | 具有殺有害生物效用之組成物及與其相關之方法 |
| BR112022027035A2 (pt) | 2020-07-14 | 2023-04-11 | Pioneer Hi Bred Int | Proteínas inseticidas e métodos para uso das mesmas |
| CN116096903A (zh) | 2020-08-10 | 2023-05-09 | 先锋国际良种公司 | 植物调节元件及其使用方法 |
| CN111965353B (zh) * | 2020-08-18 | 2022-08-19 | 四川农业大学 | 绵羊痒螨半胱氨酸蛋白酶抑制剂的应用以及一种elisa试剂盒 |
| TW202345696A (zh) | 2022-05-18 | 2023-12-01 | 美商科迪華農業科技有限責任公司 | 具有殺有害生物效用之組成物及與其相關的方法 |
| WO2025178772A1 (en) | 2024-02-23 | 2025-08-28 | Genective Sa | Insecticidal proteins compositions and methods of use |
| WO2025193453A1 (en) | 2024-03-14 | 2025-09-18 | Genective Sa | Insecticidal proteins compositions and methods of use |
| WO2025235220A1 (en) | 2024-05-08 | 2025-11-13 | Genective Sa | Insecticidal proteins compositions and methods of use |
| WO2025264577A1 (en) | 2024-06-20 | 2025-12-26 | Genective Sa | Insecticidal proteins, compositions and methods of use |
| WO2025264584A1 (en) | 2024-06-20 | 2025-12-26 | Genective Sa | Insecticidal proteins, compositions and methods of use |
| WO2026006045A1 (en) | 2024-06-25 | 2026-01-02 | Genective Sa | Insecticidal proteins, compositions and methods of use |
| WO2026043743A1 (en) | 2024-08-21 | 2026-02-26 | Genective Sa | Insecticidal proteins compositions and methods of use |
| WO2026055006A2 (en) | 2024-09-03 | 2026-03-12 | Genective Sa | Insecticidal proteins compositions and methods of use |
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| MX381370B (es) | 2025-03-12 |
| CN107108705B (zh) | 2021-05-25 |
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| US10781458B2 (en) | 2020-09-22 |
| CN107108705A (zh) | 2017-08-29 |
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| US20230056043A1 (en) | 2023-02-23 |
| RU2017116790A3 (ru) | 2019-05-17 |
| MX2017004745A (es) | 2017-10-20 |
| EP3207143A1 (en) | 2017-08-23 |
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| US10435706B2 (en) | 2019-10-08 |
| WO2016061206A1 (en) | 2016-04-21 |
| CA2963558C (en) | 2023-04-04 |
| UA126192C2 (uk) | 2022-08-31 |
| US11492641B2 (en) | 2022-11-08 |
| US20170233440A1 (en) | 2017-08-17 |
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| EP3207143B1 (en) | 2023-11-22 |
| US20200399653A1 (en) | 2020-12-24 |
| BR112017007932A2 (pt) | 2018-01-23 |
| PH12017500694A1 (en) | 2017-10-09 |
| US20200010514A1 (en) | 2020-01-09 |
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