SK287146B6 - Spôsob prípravy rekombinantného choriogonadotropínu - hCG zo vzorky - Google Patents

Spôsob prípravy rekombinantného choriogonadotropínu - hCG zo vzorky Download PDF

Info

Publication number: SK287146B6
Authority: SK; Slovakia
Prior art keywords: hcg; column; eluting; sample; chromatography
Prior art date: 2000-02-22

Application number

SK1206-2002A

Other languages

English (en)

Slovak (sk)

Other versions

SK12062002A3 (sk

Inventor

Gianfranco Paradisi

Mara Rossi

Laura Scaglia

Original Assignee

Laboratoires Serono Sa

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-02-22

Filing date

2001-01-22

Publication date

2010-01-07

2001-01-22 Application filed by Laboratoires Serono Sa filed Critical Laboratoires Serono Sa

2002-12-03 Publication of SK12062002A3 publication Critical patent/SK12062002A3/sk

2010-01-07 Publication of SK287146B6 publication Critical patent/SK287146B6/sk

Links

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238000002360 preparation method Methods 0.000 title claims abstract description 11
102000011022 Chorionic Gonadotropin Human genes 0.000 title description 5
108010062540 Chorionic Gonadotropin Proteins 0.000 title description 5
229940015047 chorionic gonadotropin Drugs 0.000 title 1
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 40
238000004255 ion exchange chromatography Methods 0.000 claims abstract description 20
239000000377 silicon dioxide Substances 0.000 claims abstract description 20
238000004007 reversed phase HPLC Methods 0.000 claims abstract description 14
238000004587 chromatography analysis Methods 0.000 claims abstract description 11
238000001542 size-exclusion chromatography Methods 0.000 claims abstract description 6
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 28
229920002271 DEAE-Sepharose Polymers 0.000 claims description 12
238000010828 elution Methods 0.000 claims description 12
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239000012506 Sephacryl® Substances 0.000 claims description 9
239000001963 growth medium Substances 0.000 claims description 9
239000012064 sodium phosphate buffer Substances 0.000 claims description 9
239000000872 buffer Substances 0.000 claims description 7
ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims description 6
229910000013 Ammonium bicarbonate Inorganic materials 0.000 claims description 6
235000012538 ammonium bicarbonate Nutrition 0.000 claims description 6
239000001099 ammonium carbonate Substances 0.000 claims description 6
238000004128 high performance liquid chromatography Methods 0.000 claims description 5
JLEXUIVKURIPFI-UHFFFAOYSA-N tris phosphate Chemical compound OP(O)(O)=O.OCC(N)(CO)CO JLEXUIVKURIPFI-UHFFFAOYSA-N 0.000 claims description 5
210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 2
NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims 2
239000003456 ion exchange resin Substances 0.000 claims 2
229920003303 ion-exchange polymer Polymers 0.000 claims 2
239000000243 solution Substances 0.000 description 23
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
238000000108 ultra-filtration Methods 0.000 description 14
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NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 11
239000000203 mixture Substances 0.000 description 10
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239000001488 sodium phosphate Substances 0.000 description 9
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210000002700 urine Anatomy 0.000 description 9
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229930195725 Mannitol Natural products 0.000 description 3
229910021529 ammonia Inorganic materials 0.000 description 3
-1 analytical grade Chemical compound 0.000 description 3
230000007717 exclusion Effects 0.000 description 3
239000012091 fetal bovine serum Substances 0.000 description 3
239000000594 mannitol Substances 0.000 description 3
235000010355 mannitol Nutrition 0.000 description 3
102000039446 nucleic acids Human genes 0.000 description 3
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239000008363 phosphate buffer Substances 0.000 description 3
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
206010058359 Hypogonadism Diseases 0.000 description 2
108010057021 Menotropins Proteins 0.000 description 2
UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
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239000005556 hormone Substances 0.000 description 2
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CBUWTGCATVNMJE-UHFFFAOYSA-N 6,6-dimethylheptanal Chemical compound CC(C)(C)CCCCC=O CBUWTGCATVNMJE-UHFFFAOYSA-N 0.000 description 1
241001135756 Alphaproteobacteria Species 0.000 description 1
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
241001135755 Betaproteobacteria Species 0.000 description 1
102000004506 Blood Proteins Human genes 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
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SGHZXLIDFTYFHQ-UHFFFAOYSA-L Brilliant Blue Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 SGHZXLIDFTYFHQ-UHFFFAOYSA-L 0.000 description 1
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206010012205 Delayed puberty Diseases 0.000 description 1
241000283086 Equidae Species 0.000 description 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
108010079345 Follicle Stimulating Hormone Proteins 0.000 description 1
102000012673 Follicle Stimulating Hormone Human genes 0.000 description 1
108010086677 Gonadotropins Proteins 0.000 description 1
102000006771 Gonadotropins Human genes 0.000 description 1
241001622557 Hesperia Species 0.000 description 1
108010073521 Luteinizing Hormone Proteins 0.000 description 1
102000009151 Luteinizing Hormone Human genes 0.000 description 1
208000007466 Male Infertility Diseases 0.000 description 1
241001494479 Pecora Species 0.000 description 1
241000282887 Suidae Species 0.000 description 1
206010042573 Superovulation Diseases 0.000 description 1
ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 1
241000700605 Viruses Species 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
239000002253 acid Substances 0.000 description 1
239000013543 active substance Substances 0.000 description 1
238000005571 anion exchange chromatography Methods 0.000 description 1
206010003883 azoospermia Diseases 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
230000008901 benefit Effects 0.000 description 1
230000004071 biological effect Effects 0.000 description 1
244000309464 bull Species 0.000 description 1
239000006143 cell culture medium Substances 0.000 description 1
239000012560 cell impurity Substances 0.000 description 1
230000008859 change Effects 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
235000019693 cherries Nutrition 0.000 description 1
238000004140 cleaning Methods 0.000 description 1
229940046989 clomiphene citrate Drugs 0.000 description 1
238000004440 column chromatography Methods 0.000 description 1
238000011109 contamination Methods 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
239000012045 crude solution Substances 0.000 description 1
201000000160 cryptorchidism Diseases 0.000 description 1
239000012228 culture supernatant Substances 0.000 description 1
ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
238000000354 decomposition reaction Methods 0.000 description 1
229940075894 denatured ethanol Drugs 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
239000000539 dimer Substances 0.000 description 1
BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
230000000694 effects Effects 0.000 description 1
239000002158 endotoxin Substances 0.000 description 1
238000011067 equilibration Methods 0.000 description 1
230000004720 fertilization Effects 0.000 description 1
229940028334 follicle stimulating hormone Drugs 0.000 description 1
230000008217 follicular development Effects 0.000 description 1
238000004108 freeze drying Methods 0.000 description 1
238000007710 freezing Methods 0.000 description 1
230000008014 freezing Effects 0.000 description 1
230000001456 gonadotroph Effects 0.000 description 1
239000002622 gonadotropin Substances 0.000 description 1
229940094892 gonadotropins Drugs 0.000 description 1
238000003306 harvesting Methods 0.000 description 1
201000003368 hypogonadotropic hypogonadism Diseases 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
208000000509 infertility Diseases 0.000 description 1
230000036512 infertility Effects 0.000 description 1
231100000535 infertility Toxicity 0.000 description 1
238000010255 intramuscular injection Methods 0.000 description 1
239000007927 intramuscular injection Substances 0.000 description 1
239000007788 liquid Substances 0.000 description 1
239000012669 liquid formulation Substances 0.000 description 1
239000012263 liquid product Substances 0.000 description 1
229940040129 luteinizing hormone Drugs 0.000 description 1
239000000463 material Substances 0.000 description 1
238000005374 membrane filtration Methods 0.000 description 1
230000003278 mimic effect Effects 0.000 description 1
230000004048 modification Effects 0.000 description 1
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229910000403 monosodium phosphate Inorganic materials 0.000 description 1
235000019799 monosodium phosphate Nutrition 0.000 description 1
208000008634 oligospermia Diseases 0.000 description 1
230000036616 oligospermia Effects 0.000 description 1
231100000528 oligospermia Toxicity 0.000 description 1
210000000287 oocyte Anatomy 0.000 description 1
239000008188 pellet Substances 0.000 description 1
239000002243 precursor Substances 0.000 description 1
230000006318 protein oxidation Effects 0.000 description 1
239000008213 purified water Substances 0.000 description 1
239000002994 raw material Substances 0.000 description 1
230000001850 reproductive effect Effects 0.000 description 1
238000004366 reverse phase liquid chromatography Methods 0.000 description 1
235000012239 silicon dioxide Nutrition 0.000 description 1
229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
235000017557 sodium bicarbonate Nutrition 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
239000007790 solid phase Substances 0.000 description 1
241000894007 species Species 0.000 description 1
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238000010186 staining Methods 0.000 description 1
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239000013589 supplement Substances 0.000 description 1
229960003604 testosterone Drugs 0.000 description 1
238000010257 thawing Methods 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
238000000870 ultraviolet spectroscopy Methods 0.000 description 1
238000005406 washing Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Endocrinology (AREA)
Medicinal Chemistry (AREA)
General Health & Medical Sciences (AREA)
Reproductive Health (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Engineering & Computer Science (AREA)
General Chemical & Material Sciences (AREA)
Pharmacology & Pharmacy (AREA)
Chemical Kinetics & Catalysis (AREA)
Animal Behavior & Ethology (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Molecular Biology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Biophysics (AREA)
Biochemistry (AREA)
Gastroenterology & Hepatology (AREA)
Zoology (AREA)
Toxicology (AREA)
Analytical Chemistry (AREA)
Gynecology & Obstetrics (AREA)
Diabetes (AREA)
Pregnancy & Childbirth (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Detergent Compositions (AREA)
Compounds Of Unknown Constitution (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Treatment Of Liquids With Adsorbents In General (AREA)

SK1206-2002A 2000-02-22 2001-01-22 Spôsob prípravy rekombinantného choriogonadotropínu - hCG zo vzorky SK287146B6 (sk)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP00103690		2000-02-22
PCT/EP2001/000665 WO2001062773A1 (fr)	2000-02-22	2001-01-22	PROCEDE DE PURIFICATION DE hCG ET hCG RECOMBINEE PURIFIEE PAR CETTE METHODE

Publications (2)

Publication Number	Publication Date
SK12062002A3 SK12062002A3 (sk)	2002-12-03
SK287146B6 true SK287146B6 (sk)	2010-01-07

Family

ID=8167925

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SK1206-2002A SK287146B6 (sk)	2000-02-22	2001-01-22	Spôsob prípravy rekombinantného choriogonadotropínu - hCG zo vzorky

Country Status (31)

Country	Link
US (2)	US7297777B2 (fr)
EP (2)	EP1752466A3 (fr)
JP (1)	JP4588960B2 (fr)
KR (1)	KR100806911B1 (fr)
CN (1)	CN100482679C (fr)
AT (1)	ATE406378T1 (fr)
AU (2)	AU783320B2 (fr)
BG (1)	BG65807B1 (fr)
BR (1)	BRPI0108556B8 (fr)
CA (1)	CA2399020A1 (fr)
CY (1)	CY1108460T1 (fr)
CZ (1)	CZ303144B6 (fr)
DE (1)	DE60135541D1 (fr)
DK (1)	DK1257564T3 (fr)
EA (1)	EA006605B1 (fr)
EE (1)	EE05644B1 (fr)
ES (1)	ES2307585T3 (fr)
HR (1)	HRP20020650B1 (fr)
HU (2)	HU1300063D0 (fr)
IL (2)	IL151308A0 (fr)
ME (1)	ME00296B (fr)
MX (1)	MXPA02007871A (fr)
NO (1)	NO328694B1 (fr)
PL (1)	PL205149B1 (fr)
PT (1)	PT1257564E (fr)
RS (1)	RS50741B (fr)
SI (1)	SI1257564T1 (fr)
SK (1)	SK287146B6 (fr)
UA (2)	UA86938C2 (fr)
WO (1)	WO2001062773A1 (fr)
ZA (1)	ZA200206109B (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL151308A0 (en) *	2000-02-22	2003-04-10	Applied Research Systems	PURIFICATION OF hCG AND PHARMACEUTICAL COMPOSITIONS CONTAINING IT
JP2007513192A (ja) *	2004-10-04	2007-05-24	ノベタイド，リミティド	ペプチド用の対イオン交換法
CN1958603B (zh) *	2005-11-04	2010-05-05	上海天伟生物制药有限公司	一种人绒毛膜促性腺素的纯化方法
EA200802426A1 (ru) *	2006-07-11	2009-04-28	Коваль, Антон Александрович	Применение хорионического или лютеинизирующего гормона для профилактики или лечения возрастного гипогонадизма
CN101397339B (zh) *	2008-09-24	2012-07-25	上海天伟生物制药有限公司	糖蛋白中去除/灭活病毒的方法
TWI532495B (zh) *	2009-10-05	2016-05-11	菲瑞茵國際中心股份有限公司	藥學製劑
CN101792481B (zh) *	2010-03-12	2012-05-30	丽珠医药集团股份有限公司	一种绒毛膜促性腺激素的纯化方法
WO2012120535A2 (fr) *	2011-02-03	2012-09-13	Sanzyme Limited	Composition contenant de la gonadotropine chorionique extrêmement purifiée, sa formulation et ses utilisations
US9757469B2 (en)	2011-03-31	2017-09-12	Ferring B.V.	Pharmaceutical preparation
WO2013102921A2 (fr) *	2011-11-17	2013-07-11	Sanzyme Limited	Hcg - nouvelle modalité de traitement du diabète de type 2 (t2dm)
CN103288950A (zh) *	2012-12-28	2013-09-11	青岛九龙生物医药有限公司	改进柱分离工序洗涤液和洗脱液配比去除杂质的方法
CN104101656A (zh) *	2013-04-01	2014-10-15	上海天伟生物制药有限公司	一种促性腺激素的纯度测定方法
US20150065426A1 (en) *	2013-08-27	2015-03-05	Professional Compounding Centers Of America	Testosterone Booster Transdermal Compositions
CN105968185A (zh) *	2016-07-20	2016-09-28	宁波人健药业集团股份有限公司	一种绒促性素纯化方法
CN111233999B (zh) *	2020-03-21	2024-02-23	上海浦东明炎生物技术有限公司	一种从人尿中提取人绒毛膜促性腺激素的方法
CN113398251B (zh) *	2021-07-30	2022-08-05	宁波人健药业集团股份有限公司	一种重组人绒毛膜促性腺激素冻干粉针剂及其制备方法
CN116063452A (zh) *	2023-01-03	2023-05-05	杭州浦泰生物科技有限公司	一种人绒毛膜促性腺激素高效纯化方法

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- 2001-01-22 US US10/204,630 patent/US7297777B2/en not_active Expired - Lifetime
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Also Published As

Publication number	Publication date
HU1300063D0 (hu)	2003-03-28
EE05644B1 (et)	2013-04-15
MEP38908A (en)	2011-02-10
NO20023985D0 (no)	2002-08-21
US20070299001A1 (en)	2007-12-27
NO20023985L (no)	2002-08-21
DK1257564T3 (da)	2008-10-06
EE200200469A (et)	2004-04-15
EP1752466A2 (fr)	2007-02-14
PT1257564E (pt)	2008-09-10
CZ303144B6 (cs)	2012-05-02
EP1257564B1 (fr)	2008-08-27
HK1052016A1 (zh)	2003-08-29
PL205149B1 (pl)	2010-03-31
HUP0204231A3 (en)	2005-09-28
ME00296B (fr)	2011-05-10
EP1752466A3 (fr)	2012-10-10
SK12062002A3 (sk)	2002-12-03
US20030104553A1 (en)	2003-06-05
CN100482679C (zh)	2009-04-29
HUP0204231A2 (hu)	2003-03-28
WO2001062773A1 (fr)	2001-08-30
UA78488C2 (en)	2007-04-10
RS50741B (sr)	2010-08-31
EA200200892A1 (ru)	2003-02-27
IL151308A (en)	2009-11-18
AU2680401A (en)	2001-09-03
BG65807B1 (bg)	2009-12-31
HRP20020650A2 (en)	2004-12-31
MXPA02007871A (es)	2003-02-10
SI1257564T1 (sl)	2008-12-31
CY1108460T1 (el)	2014-04-09
ZA200206109B (en)	2003-07-31
JP2003524015A (ja)	2003-08-12
BRPI0108556B8 (pt)	2021-05-25
UA86938C2 (uk)	2009-06-10
PL357508A1 (en)	2004-07-26
CZ20022855A3 (cs)	2002-11-13
CA2399020A1 (fr)	2001-08-30
YU60202A (sh)	2005-09-19
BG107000A (en)	2003-04-30
HRP20020650B1 (en)	2011-02-28
US7297777B2 (en)	2007-11-20
AU2006200059A1 (en)	2006-02-02
HU228935B1 (en)	2013-06-28
CN1404485A (zh)	2003-03-19
NO328694B1 (no)	2010-04-26
KR20030001359A (ko)	2003-01-06
EP1257564A1 (fr)	2002-11-20
BR0108556A (pt)	2003-01-28
BRPI0108556B1 (pt)	2015-04-14
EA006605B1 (ru)	2006-02-24
ATE406378T1 (de)	2008-09-15
AU783320B2 (en)	2005-10-13
ES2307585T3 (es)	2008-12-01
IL151308A0 (en)	2003-04-10
DE60135541D1 (de)	2008-10-09
JP4588960B2 (ja)	2010-12-01
KR100806911B1 (ko)	2008-02-22

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2012-08-06

TC4A

Change of owner's name

Owner name: MERCK SERONO SA, COINSINS, VAUD, CH

Effective date: 20120709

2021-02-10

MK4A

Patent expired

Expiry date: 20210122

Publication	Publication Date	Title
US20070299001A1 (en)	2007-12-27	PURIFIED hCG
RS60202B1 (sr)	2020-06-30	Jedinjenja cikličnog etra pirazol-4-il-heterociklil-karboksamida i postupci primene
US7053054B2 (en)	2006-05-30	Purified LH
HK1052016B (en)	2009-08-28	Process of purification of hcg