SK287932B6 - Magnetic glass particles, method for their preparation and uses thereof - Google Patents
Magnetic glass particles, method for their preparation and uses thereof Download PDFInfo
- Publication number
- SK287932B6 SK287932B6 SK664-2002A SK6642002A SK287932B6 SK 287932 B6 SK287932 B6 SK 287932B6 SK 6642002 A SK6642002 A SK 6642002A SK 287932 B6 SK287932 B6 SK 287932B6
- Authority
- SK
- Slovakia
- Prior art keywords
- magnetic
- suspension
- particles
- mgp
- nucleic acid
- Prior art date
Links
- 239000002245 particle Substances 0.000 title claims abstract description 143
- 238000000034 method Methods 0.000 title claims abstract description 139
- 239000011521 glass Substances 0.000 title claims abstract description 110
- 230000005291 magnetic effect Effects 0.000 title claims abstract description 105
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 239000000725 suspension Substances 0.000 claims abstract description 63
- 239000006249 magnetic particle Substances 0.000 claims abstract description 42
- 238000000746 purification Methods 0.000 claims abstract description 24
- 150000007523 nucleic acids Chemical class 0.000 claims description 107
- 239000000203 mixture Substances 0.000 claims description 101
- 102000039446 nucleic acids Human genes 0.000 claims description 95
- 108020004707 nucleic acids Proteins 0.000 claims description 95
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 88
- 108020004414 DNA Proteins 0.000 claims description 71
- 239000007921 spray Substances 0.000 claims description 57
- 238000006243 chemical reaction Methods 0.000 claims description 52
- 239000012620 biological material Substances 0.000 claims description 48
- 238000001514 detection method Methods 0.000 claims description 47
- 239000000243 solution Substances 0.000 claims description 43
- 238000003752 polymerase chain reaction Methods 0.000 claims description 42
- 108091034117 Oligonucleotide Proteins 0.000 claims description 38
- 230000008569 process Effects 0.000 claims description 37
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 35
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 28
- 230000003321 amplification Effects 0.000 claims description 27
- 150000003839 salts Chemical class 0.000 claims description 27
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 26
- 230000000694 effects Effects 0.000 claims description 24
- 238000004062 sedimentation Methods 0.000 claims description 24
- 230000003196 chaotropic effect Effects 0.000 claims description 20
- 238000001694 spray drying Methods 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 18
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 claims description 15
- 239000003153 chemical reaction reagent Substances 0.000 claims description 15
- 239000002773 nucleotide Substances 0.000 claims description 15
- 125000003729 nucleotide group Chemical group 0.000 claims description 15
- 238000004519 manufacturing process Methods 0.000 claims description 14
- 238000012360 testing method Methods 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 12
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- 229910052742 iron Inorganic materials 0.000 claims description 11
- 238000000926 separation method Methods 0.000 claims description 11
- 108091023037 Aptamer Proteins 0.000 claims description 9
- 238000005245 sintering Methods 0.000 claims description 9
- 230000005294 ferromagnetic effect Effects 0.000 claims description 8
- 230000000903 blocking effect Effects 0.000 claims description 7
- 230000000295 complement effect Effects 0.000 claims description 7
- 239000002904 solvent Substances 0.000 claims description 7
- 238000003860 storage Methods 0.000 claims description 7
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 6
- 230000007062 hydrolysis Effects 0.000 claims description 5
- 238000006460 hydrolysis reaction Methods 0.000 claims description 5
- 108010037497 3'-nucleotidase Proteins 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 4
- 230000001419 dependent effect Effects 0.000 claims description 4
- 238000010494 dissociation reaction Methods 0.000 claims description 4
- 230000005593 dissociations Effects 0.000 claims description 4
- 239000003480 eluent Substances 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 239000012634 fragment Substances 0.000 claims description 3
- 239000012456 homogeneous solution Substances 0.000 claims description 2
- 238000007837 multiplex assay Methods 0.000 claims description 2
- 230000036961 partial effect Effects 0.000 claims description 2
- 241000209219 Hordeum Species 0.000 claims 2
- 235000007340 Hordeum vulgare Nutrition 0.000 claims 2
- 230000037452 priming Effects 0.000 claims 1
- 239000000523 sample Substances 0.000 description 94
- 102000053602 DNA Human genes 0.000 description 70
- 239000013615 primer Substances 0.000 description 45
- 239000000049 pigment Substances 0.000 description 44
- 229920002477 rna polymer Polymers 0.000 description 37
- 241000725303 Human immunodeficiency virus Species 0.000 description 31
- 241000711549 Hepacivirus C Species 0.000 description 29
- 239000002585 base Substances 0.000 description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 28
- 239000012530 fluid Substances 0.000 description 24
- 238000009739 binding Methods 0.000 description 22
- 229910004298 SiO 2 Inorganic materials 0.000 description 21
- 230000027455 binding Effects 0.000 description 21
- 238000002474 experimental method Methods 0.000 description 21
- -1 B 2 O 3 Inorganic materials 0.000 description 20
- 238000011534 incubation Methods 0.000 description 20
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 19
- 238000001212 derivatisation Methods 0.000 description 19
- 239000000872 buffer Substances 0.000 description 18
- 239000011162 core material Substances 0.000 description 18
- 238000005259 measurement Methods 0.000 description 17
- 238000005406 washing Methods 0.000 description 17
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 16
- 239000000463 material Substances 0.000 description 16
- 239000011734 sodium Substances 0.000 description 16
- 239000000126 substance Substances 0.000 description 16
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 15
- 239000011324 bead Substances 0.000 description 15
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 14
- 229910018072 Al 2 O 3 Inorganic materials 0.000 description 13
- 239000006228 supernatant Substances 0.000 description 13
- 238000003556 assay Methods 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 12
- 239000011159 matrix material Substances 0.000 description 12
- 239000008279 sol Substances 0.000 description 12
- 241000700605 Viruses Species 0.000 description 11
- 238000002955 isolation Methods 0.000 description 11
- 230000002441 reversible effect Effects 0.000 description 11
- 241000282414 Homo sapiens Species 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 description 10
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 description 10
- 238000001035 drying Methods 0.000 description 10
- 238000000605 extraction Methods 0.000 description 10
- 230000001965 increasing effect Effects 0.000 description 10
- 239000012139 lysis buffer Substances 0.000 description 10
- 239000000499 gel Substances 0.000 description 9
- 238000009396 hybridization Methods 0.000 description 9
- 239000000377 silicon dioxide Substances 0.000 description 9
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 9
- 239000011534 wash buffer Substances 0.000 description 9
- 239000007983 Tris buffer Substances 0.000 description 8
- 230000008033 biological extinction Effects 0.000 description 8
- 229910052757 nitrogen Inorganic materials 0.000 description 8
- 238000001179 sorption measurement Methods 0.000 description 8
- 238000002123 RNA extraction Methods 0.000 description 7
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 7
- 230000008901 benefit Effects 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 7
- 229960002685 biotin Drugs 0.000 description 7
- 235000020958 biotin Nutrition 0.000 description 7
- 239000011616 biotin Substances 0.000 description 7
- 239000000356 contaminant Substances 0.000 description 7
- 238000010790 dilution Methods 0.000 description 7
- 239000012895 dilution Substances 0.000 description 7
- 238000010828 elution Methods 0.000 description 7
- 239000007789 gas Substances 0.000 description 7
- 239000006148 magnetic separator Substances 0.000 description 7
- 238000005507 spraying Methods 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 7
- 238000011144 upstream manufacturing Methods 0.000 description 7
- 230000003612 virological effect Effects 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 229910052681 coesite Inorganic materials 0.000 description 6
- 229910052906 cristobalite Inorganic materials 0.000 description 6
- 230000009089 cytolysis Effects 0.000 description 6
- 230000007423 decrease Effects 0.000 description 6
- 238000007885 magnetic separation Methods 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 6
- 230000002285 radioactive effect Effects 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 6
- 235000012239 silicon dioxide Nutrition 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 229910052682 stishovite Inorganic materials 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 229910052905 tridymite Inorganic materials 0.000 description 6
- 239000001226 triphosphate Substances 0.000 description 6
- 235000011178 triphosphate Nutrition 0.000 description 6
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical class C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
- 108010067770 Endopeptidase K Proteins 0.000 description 5
- BOTDANWDWHJENH-UHFFFAOYSA-N Tetraethyl orthosilicate Chemical compound CCO[Si](OCC)(OCC)OCC BOTDANWDWHJENH-UHFFFAOYSA-N 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- 150000001298 alcohols Chemical class 0.000 description 5
- 239000012491 analyte Substances 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 238000009795 derivation Methods 0.000 description 5
- 239000012154 double-distilled water Substances 0.000 description 5
- 229960001484 edetic acid Drugs 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 235000013980 iron oxide Nutrition 0.000 description 5
- 239000010445 mica Substances 0.000 description 5
- 229910052618 mica group Inorganic materials 0.000 description 5
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 5
- 239000011148 porous material Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 238000000163 radioactive labelling Methods 0.000 description 5
- 230000002829 reductive effect Effects 0.000 description 5
- 125000006853 reporter group Chemical group 0.000 description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- 239000003463 adsorbent Substances 0.000 description 4
- 238000013459 approach Methods 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 4
- 238000002825 functional assay Methods 0.000 description 4
- 230000005484 gravity Effects 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 150000001282 organosilanes Chemical class 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000011550 stock solution Substances 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 3
- 229910010413 TiO 2 Inorganic materials 0.000 description 3
- 108010020713 Tth polymerase Proteins 0.000 description 3
- 230000004931 aggregating effect Effects 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 229910001566 austenite Inorganic materials 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 239000012149 elution buffer Substances 0.000 description 3
- 238000006911 enzymatic reaction Methods 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 239000011888 foil Substances 0.000 description 3
- 239000003365 glass fiber Substances 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000001198 high resolution scanning electron microscopy Methods 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 150000002576 ketones Chemical class 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 239000002987 primer (paints) Substances 0.000 description 3
- 238000010839 reverse transcription Methods 0.000 description 3
- 238000003757 reverse transcription PCR Methods 0.000 description 3
- 235000009518 sodium iodide Nutrition 0.000 description 3
- BAZAXWOYCMUHIX-UHFFFAOYSA-M sodium perchlorate Chemical compound [Na+].[O-]Cl(=O)(=O)=O BAZAXWOYCMUHIX-UHFFFAOYSA-M 0.000 description 3
- 229910001488 sodium perchlorate Inorganic materials 0.000 description 3
- 230000009870 specific binding Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 3
- 239000012224 working solution Substances 0.000 description 3
- 239000011701 zinc Substances 0.000 description 3
- 208000023275 Autoimmune disease Diseases 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 241000700721 Hepatitis B virus Species 0.000 description 2
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 102000001253 Protein Kinase Human genes 0.000 description 2
- 229910003902 SiCl 4 Inorganic materials 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 2
- ZOIORXHNWRGPMV-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O.CC(O)=O ZOIORXHNWRGPMV-UHFFFAOYSA-N 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 150000004645 aluminates Chemical class 0.000 description 2
- SMZOGRDCAXLAAR-UHFFFAOYSA-N aluminium isopropoxide Chemical compound [Al+3].CC(C)[O-].CC(C)[O-].CC(C)[O-] SMZOGRDCAXLAAR-UHFFFAOYSA-N 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 235000010216 calcium carbonate Nutrition 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000008139 complexing agent Substances 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000010419 fine particle Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 229960000789 guanidine hydrochloride Drugs 0.000 description 2
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 2
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 2
- 239000001307 helium Substances 0.000 description 2
- 229910052734 helium Inorganic materials 0.000 description 2
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 2
- 238000001889 high-resolution electron micrograph Methods 0.000 description 2
- 150000004679 hydroxides Chemical class 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000009830 intercalation Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 238000010907 mechanical stirring Methods 0.000 description 2
- 229910044991 metal oxide Inorganic materials 0.000 description 2
- 150000004706 metal oxides Chemical class 0.000 description 2
- 239000004005 microsphere Substances 0.000 description 2
- 238000001821 nucleic acid purification Methods 0.000 description 2
- 239000002777 nucleoside Substances 0.000 description 2
- NDLPOXTZKUMGOV-UHFFFAOYSA-N oxo(oxoferriooxy)iron hydrate Chemical compound O.O=[Fe]O[Fe]=O NDLPOXTZKUMGOV-UHFFFAOYSA-N 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
- 238000000159 protein binding assay Methods 0.000 description 2
- 108060006633 protein kinase Proteins 0.000 description 2
- 239000012925 reference material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 238000003980 solgel method Methods 0.000 description 2
- 239000007962 solid dispersion Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000012876 topography Methods 0.000 description 2
- AJSTXXYNEIHPMD-UHFFFAOYSA-N triethyl borate Chemical compound CCOB(OCC)OCC AJSTXXYNEIHPMD-UHFFFAOYSA-N 0.000 description 2
- 239000004246 zinc acetate Substances 0.000 description 2
- 235000013904 zinc acetate Nutrition 0.000 description 2
- 229960000314 zinc acetate Drugs 0.000 description 2
- 229910000859 α-Fe Inorganic materials 0.000 description 2
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 1
- IEQAICDLOKRSRL-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-dodecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO IEQAICDLOKRSRL-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- BMYCCWYAFNPAQC-UHFFFAOYSA-N 2-[dodecyl(methyl)azaniumyl]acetate Chemical compound CCCCCCCCCCCCN(C)CC(O)=O BMYCCWYAFNPAQC-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 125000000954 2-hydroxyethyl group Chemical group [H]C([*])([H])C([H])([H])O[H] 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 238000004438 BET method Methods 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 229910001369 Brass Inorganic materials 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 description 1
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 241001524679 Escherichia virus M13 Species 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 229910017061 Fe Co Inorganic materials 0.000 description 1
- 229910005793 GeO 2 Inorganic materials 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 208000028782 Hereditary disease Diseases 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 229910020451 K2SiO3 Inorganic materials 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 208000024556 Mendelian disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- BACYUWVYYTXETD-UHFFFAOYSA-N N-Lauroylsarcosine Chemical compound CCCCCCCCCCCC(=O)N(C)CC(O)=O BACYUWVYYTXETD-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- FTJDMCFKMFIQQN-UHFFFAOYSA-N P(=O)(O)(O)O.OCN(CO)CO Chemical compound P(=O)(O)(O)O.OCN(CO)CO FTJDMCFKMFIQQN-UHFFFAOYSA-N 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 238000010802 RNA extraction kit Methods 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 229910008051 Si-OH Inorganic materials 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 229910006358 Si—OH Inorganic materials 0.000 description 1
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 239000007997 Tricine buffer Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Natural products O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 1
- GEIAQOFPUVMAGM-UHFFFAOYSA-N ZrO Inorganic materials [Zr]=O GEIAQOFPUVMAGM-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001251 acridines Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 229910001413 alkali metal ion Inorganic materials 0.000 description 1
- 150000001340 alkali metals Chemical class 0.000 description 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 1
- 229910001420 alkaline earth metal ion Inorganic materials 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 238000001636 atomic emission spectroscopy Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 150000001642 boronic acid derivatives Chemical class 0.000 description 1
- 239000010951 brass Substances 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 1
- 239000001639 calcium acetate Substances 0.000 description 1
- 235000011092 calcium acetate Nutrition 0.000 description 1
- 229960005147 calcium acetate Drugs 0.000 description 1
- 239000003990 capacitor Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000012568 clinical material Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- KPLQYGBQNPPQGA-UHFFFAOYSA-N cobalt samarium Chemical compound [Co].[Sm] KPLQYGBQNPPQGA-UHFFFAOYSA-N 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000011258 core-shell material Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000001955 cumulated effect Effects 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 238000001739 density measurement Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000003891 environmental analysis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- CCIVGXIOQKPBKL-UHFFFAOYSA-M ethanesulfonate Chemical compound CCS([O-])(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-M 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000005293 ferrimagnetic effect Effects 0.000 description 1
- 239000003302 ferromagnetic material Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000005308 flint glass Substances 0.000 description 1
- 238000004186 food analysis Methods 0.000 description 1
- 238000011990 functional testing Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 238000001239 high-resolution electron microscopy Methods 0.000 description 1
- 238000007849 hot-start PCR Methods 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- VBMVTYDPPZVILR-UHFFFAOYSA-N iron(2+);oxygen(2-) Chemical class [O-2].[Fe+2] VBMVTYDPPZVILR-UHFFFAOYSA-N 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 229920001684 low density polyethylene Polymers 0.000 description 1
- 239000004702 low-density polyethylene Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 238000002826 magnetic-activated cell sorting Methods 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 230000005415 magnetization Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 239000011325 microbead Substances 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 239000012299 nitrogen atmosphere Substances 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 description 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000011197 physicochemical method Methods 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000005373 porous glass Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 150000003138 primary alcohols Chemical class 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 229910000938 samarium–cobalt magnet Inorganic materials 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 108700004121 sarkosyl Proteins 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 150000003333 secondary alcohols Chemical class 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 208000007056 sickle cell anemia Diseases 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 125000005372 silanol group Chemical group 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000005368 silicate glass Substances 0.000 description 1
- 150000004760 silicates Chemical class 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000003746 solid phase reaction Methods 0.000 description 1
- 239000012798 spherical particle Substances 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000004441 surface measurement Methods 0.000 description 1
- 150000003509 tertiary alcohols Chemical class 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 238000007669 thermal treatment Methods 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 229910001428 transition metal ion Inorganic materials 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000001392 ultraviolet--visible--near infrared spectroscopy Methods 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/005—Pretreatment specially adapted for magnetic separation
- B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y25/00—Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance
-
- C—CHEMISTRY; METALLURGY
- C03—GLASS; MINERAL OR SLAG WOOL
- C03B—MANUFACTURE, SHAPING, OR SUPPLEMENTARY PROCESSES
- C03B19/00—Other methods of shaping glass
- C03B19/10—Forming beads
- C03B19/1005—Forming solid beads
- C03B19/106—Forming solid beads by chemical vapour deposition; by liquid phase reaction
- C03B19/1065—Forming solid beads by chemical vapour deposition; by liquid phase reaction by liquid phase reactions, e.g. by means of a gel phase
-
- C—CHEMISTRY; METALLURGY
- C03—GLASS; MINERAL OR SLAG WOOL
- C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
- C03C1/00—Ingredients generally applicable to manufacture of glasses, glazes, or vitreous enamels
- C03C1/006—Ingredients generally applicable to manufacture of glasses, glazes, or vitreous enamels to produce glass through wet route
-
- C—CHEMISTRY; METALLURGY
- C03—GLASS; MINERAL OR SLAG WOOL
- C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
- C03C12/00—Powdered glass; Bead compositions
-
- C—CHEMISTRY; METALLURGY
- C03—GLASS; MINERAL OR SLAG WOOL
- C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
- C03C14/00—Glass compositions containing a non-glass component, e.g. compositions containing fibres, filaments, whiskers, platelets, or the like, dispersed in a glass matrix
- C03C14/004—Glass compositions containing a non-glass component, e.g. compositions containing fibres, filaments, whiskers, platelets, or the like, dispersed in a glass matrix the non-glass component being in the form of particles or flakes
-
- C—CHEMISTRY; METALLURGY
- C03—GLASS; MINERAL OR SLAG WOOL
- C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
- C03C3/00—Glass compositions
- C03C3/04—Glass compositions containing silica
- C03C3/076—Glass compositions containing silica with 40% to 90% silica, by weight
- C03C3/089—Glass compositions containing silica with 40% to 90% silica, by weight containing boron
- C03C3/091—Glass compositions containing silica with 40% to 90% silica, by weight containing boron containing aluminium
-
- C—CHEMISTRY; METALLURGY
- C03—GLASS; MINERAL OR SLAG WOOL
- C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
- C03C3/00—Glass compositions
- C03C3/04—Glass compositions containing silica
- C03C3/076—Glass compositions containing silica with 40% to 90% silica, by weight
- C03C3/089—Glass compositions containing silica with 40% to 90% silica, by weight containing boron
- C03C3/091—Glass compositions containing silica with 40% to 90% silica, by weight containing boron containing aluminium
- C03C3/093—Glass compositions containing silica with 40% to 90% silica, by weight containing boron containing aluminium containing zinc or zirconium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23C—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
- C23C26/00—Coating not provided for in groups C23C2/00 - C23C24/00
-
- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23C—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
- C23C30/00—Coating with metallic material characterised only by the composition of the metallic material, i.e. not characterised by the coating process
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
- H01F1/00—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
- H01F1/0036—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties showing low dimensional magnetism, i.e. spin rearrangements due to a restriction of dimensions, e.g. showing giant magnetoresistivity
- H01F1/0045—Zero dimensional, e.g. nanoparticles, soft nanoparticles for medical/biological use
- H01F1/0054—Coated nanoparticles, e.g. nanoparticles coated with organic surfactant
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
- H01F1/00—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
- H01F1/0036—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties showing low dimensional magnetism, i.e. spin rearrangements due to a restriction of dimensions, e.g. showing giant magnetoresistivity
- H01F1/0045—Zero dimensional, e.g. nanoparticles, soft nanoparticles for medical/biological use
- H01F1/0063—Zero dimensional, e.g. nanoparticles, soft nanoparticles for medical/biological use in a non-magnetic matrix, e.g. granular solids
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
- H01F1/00—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
- H01F1/44—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of magnetic liquids, e.g. ferrofluids
-
- C—CHEMISTRY; METALLURGY
- C03—GLASS; MINERAL OR SLAG WOOL
- C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
- C03C2214/00—Nature of the non-vitreous component
- C03C2214/08—Metals
-
- C—CHEMISTRY; METALLURGY
- C03—GLASS; MINERAL OR SLAG WOOL
- C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
- C03C2214/00—Nature of the non-vitreous component
- C03C2214/32—Nature of the non-vitreous component comprising a sol-gel process
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/101—Taqman
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Nanotechnology (AREA)
- Geochemistry & Mineralogy (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Power Engineering (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Dispersion Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Metallurgy (AREA)
- Mechanical Engineering (AREA)
- Plant Pathology (AREA)
- Ceramic Engineering (AREA)
- Manufacturing & Machinery (AREA)
- Inorganic Chemistry (AREA)
- Glass Compositions (AREA)
- Saccharide Compounds (AREA)
- Glass Melting And Manufacturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Hard Magnetic Materials (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP99122853 | 1999-11-17 | ||
| EP00110165A EP1154443A1 (fr) | 2000-05-12 | 2000-05-12 | Particules magnétiques de verre, méthode de fabrication et utilisation |
| PCT/EP2000/011459 WO2001037291A1 (fr) | 1999-11-17 | 2000-11-17 | Particules de verre magnetiques, procede de preparation et utilisations |
| US10/147,679 US20030224366A1 (en) | 1999-11-17 | 2002-05-16 | Magnetic glass particles, method for their preparation and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SK6642002A3 SK6642002A3 (en) | 2003-08-05 |
| SK287932B6 true SK287932B6 (sk) | 2012-05-03 |
Family
ID=32096539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SK664-2002A SK287932B6 (sk) | 1999-11-17 | 2000-11-17 | Magnetic glass particles, method for their preparation and uses thereof |
Country Status (15)
| Country | Link |
|---|---|
| US (3) | US20030224366A1 (fr) |
| EP (1) | EP1232502B1 (fr) |
| JP (2) | JP2003514383A (fr) |
| AT (1) | ATE315826T1 (fr) |
| CA (1) | CA2392009C (fr) |
| CY (1) | CY1105229T1 (fr) |
| CZ (1) | CZ20021608A3 (fr) |
| DE (1) | DE60025529T2 (fr) |
| DK (1) | DK1232502T3 (fr) |
| ES (1) | ES2256068T3 (fr) |
| HU (1) | HUP0203386A3 (fr) |
| PL (1) | PL202358B1 (fr) |
| PT (1) | PT1232502E (fr) |
| SK (1) | SK287932B6 (fr) |
| WO (1) | WO2001037291A1 (fr) |
Families Citing this family (94)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| PT1232502E (pt) | 1999-11-17 | 2006-05-31 | Roche Diagnostics Gmbh | Particulas de vidro magneticas, metodo para a sua preparacao e suas utilizacoes |
| DE10140089A1 (de) * | 2001-08-16 | 2003-02-27 | Degussa | Superparamagnetische oxidische Partikel, Verfahren zu deren Herstellung und ihre Verwendung |
| DE10153547A1 (de) | 2001-10-30 | 2003-05-22 | Degussa | Dispersion, enthaltend pyrogen hergestellte Abrasivpartikel mit superparamagnetischen Domänen |
| WO2003057910A2 (fr) | 2002-01-08 | 2003-07-17 | Roche Diagnostics Gmbh | Utilisation d'un materiau de silice dans une reaction d'amplification |
| DE10201084A1 (de) * | 2002-01-14 | 2003-07-24 | Bayer Ag | Siliziumhaltige Magnetpartikel, Verfahren zu deren Herstellung und Verwendung der Partikel |
| EP1376129B1 (fr) * | 2002-06-27 | 2007-10-10 | Toyo Boseki Kabushiki Kaisha | Supports magnétiques pour substances biologiques, leur production et leur utilisation pour isoler ces substances |
| GB0215185D0 (en) | 2002-07-01 | 2002-08-07 | Genovision As | Binding a target substance |
| US9394332B2 (en) | 2002-08-29 | 2016-07-19 | Epigenomics Ag | Method for bisulfite treatment |
| EP1394172A1 (fr) * | 2002-08-29 | 2004-03-03 | Boehringer Mannheim Gmbh | Procédé amélioré de traitement par bisulphite |
| US7597936B2 (en) | 2002-11-26 | 2009-10-06 | University Of Utah Research Foundation | Method of producing a pigmented composite microporous material |
| US7682688B2 (en) * | 2002-11-26 | 2010-03-23 | University Of Utah Research Foundation | Microporous materials, methods, and articles for localizing and quantifying analytes |
| US7560231B2 (en) | 2002-12-20 | 2009-07-14 | Roche Molecular Systems, Inc. | Mannitol and glucitol derivatives |
| WO2004067545A1 (fr) | 2003-01-29 | 2004-08-12 | Roche Diagnostics Gmbh | Procede ameliore pour traiter l'hydrogenosulfite |
| JP3644641B2 (ja) * | 2003-03-07 | 2005-05-11 | 株式会社日立ハイテクノロジーズ | 核酸回収方法 |
| CA2463719A1 (fr) | 2003-04-05 | 2004-10-05 | F. Hoffmann-La Roche Ag | Analogues de nucleotides possedant des cycles a six chainons |
| CA2482097C (fr) | 2003-10-13 | 2012-02-21 | F. Hoffmann-La Roche Ag | Methodes pour isoler des acides nucleiques |
| EP1524317B1 (fr) * | 2003-10-13 | 2015-03-04 | Roche Diagnostics GmbH | Procédé pour l'isolation d'acides nucléiques |
| WO2005054502A1 (fr) | 2003-12-02 | 2005-06-16 | Roche Diagnostics Gmbh | Procede ameliore de traitement au bisulfite |
| US7727710B2 (en) | 2003-12-24 | 2010-06-01 | 3M Innovative Properties Company | Materials, methods, and kits for reducing nonspecific binding of molecules to a surface |
| US7939249B2 (en) | 2003-12-24 | 2011-05-10 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
| EP2251441B1 (fr) | 2004-02-10 | 2013-05-08 | F. Hoffmann-La Roche AG | Nouvelles amorces et sondes pour la détection du parvovirus B19 |
| US7939251B2 (en) | 2004-05-06 | 2011-05-10 | Roche Molecular Systems, Inc. | SENP1 as a marker for cancer |
| EP1632578A1 (fr) | 2004-09-03 | 2006-03-08 | Roche Diagnostics GmbH | Méthode pour la décontamination de l' ADN |
| EP1642648A1 (fr) | 2004-09-30 | 2006-04-05 | Roche Diagnostics GmbH | Dispositif et proeédé pour regler la temperature d'un liquide |
| US7842794B2 (en) | 2004-12-17 | 2010-11-30 | Roche Molecular Systems, Inc. | Reagents and methods for detecting Neisseria gonorrhoeae |
| EP1749892B1 (fr) | 2005-08-02 | 2008-03-19 | Roche Diagnostics GmbH | Séquence nucléotidique permettant d'évaluer TSE |
| US7249521B1 (en) | 2005-08-24 | 2007-07-31 | Peter Frasca | Method for determining the presence of a magnetically susceptible or magnetic material in a sample |
| US7569367B2 (en) | 2005-09-07 | 2009-08-04 | Roche Diagnostics Operations, Inc. | Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy |
| US7981606B2 (en) | 2005-12-21 | 2011-07-19 | Roche Molecular Systems, Inc. | Control for nucleic acid testing |
| EP1932913B1 (fr) | 2006-12-11 | 2013-01-16 | Roche Diagnostics GmbH | Isolation d'acide nucléique utilisant du polidocanol et des dérivés |
| CN102776173B (zh) | 2006-12-11 | 2014-10-29 | 霍夫曼-拉罗奇有限公司 | 使用聚多卡醇和衍生物的核酸分离 |
| ES2394815T3 (es) | 2006-12-13 | 2013-02-05 | F. Hoffmann-La Roche Ag | Utilización de TDE para el aislamiento de ácidos nucleicos |
| EP2126074B1 (fr) | 2006-12-13 | 2010-12-01 | Roche Diagnostics GmbH | Utilisation des acétals pour isolation des acides nucleic |
| US8535888B2 (en) | 2006-12-29 | 2013-09-17 | Mayo Foundation For Medical Education And Research | Compositions and methods for detecting methicillin-resistant S. aureus |
| WO2008134472A1 (fr) * | 2007-04-25 | 2008-11-06 | 3M Innovative Properties Company | Compositions, méthodes et dispositifs servant à isoler des matériels biologiques |
| US20100129878A1 (en) * | 2007-04-25 | 2010-05-27 | Parthasarathy Ranjani V | Methods for nucleic acid amplification |
| DE102007063102B4 (de) | 2007-12-28 | 2022-02-10 | Robert Bosch Gmbh | Verfahren zur Erfassung eines periodisch pulsierenden Betriebsparameters |
| WO2009129236A2 (fr) * | 2008-04-14 | 2009-10-22 | Integrated Nano-Technologies Llc | Procédé pour manipuler des échantillons avec des nanoparticules de nucléation magnétiques |
| US20090258368A1 (en) * | 2008-04-14 | 2009-10-15 | Noonan John M | Paramagnetic nucleation nanoparticles |
| ES2439951T3 (es) | 2008-06-06 | 2014-01-27 | F. Hoffmann-La Roche Ag | Detección y cuantificación multiplex de ácidos nucleicos microbianos controlada de forma interna |
| EP2177271B8 (fr) | 2008-10-15 | 2019-12-18 | F. Hoffmann-La Roche AG | Système de séparation magnétique comportant des tiges magnétiques flexibles et procédé correspontant |
| CA2688174C (fr) | 2008-12-19 | 2018-08-07 | F. Hoffmann-La Roche Ag | Composition seche de reactifs comprenant de la polymerase stabilisee |
| WO2010102301A1 (fr) * | 2009-03-06 | 2010-09-10 | Jennifer Hollenstein | Procédé et appareil de mise en suspension de microparticules magnétiques |
| DE102009058650A1 (de) | 2009-12-16 | 2011-06-22 | Leibniz-Institut für Neue Materialien gemeinnützige GmbH, 66123 | Magnetische Kompositpartikel |
| US20120258553A1 (en) * | 2009-12-23 | 2012-10-11 | Koninklijke Philips Electronics N.V. | Analyte measurement apparatus and method |
| US8865000B2 (en) | 2010-06-11 | 2014-10-21 | Basf Se | Utilization of the naturally occurring magnetic constituents of ores |
| EP2579987B1 (fr) * | 2010-06-11 | 2020-03-18 | Basf Se | Utilisation des composants magnétiques natifs de minerais |
| EP2423688B1 (fr) | 2010-06-22 | 2016-03-23 | F. Hoffmann-La Roche AG | Conteneur de suspension pour des particules de liaison pour l'isolation de matériel biologique |
| EP2598653B1 (fr) | 2010-07-29 | 2017-08-23 | F. Hoffmann-La Roche AG | Pcr générique |
| US8609340B2 (en) | 2010-07-29 | 2013-12-17 | Roche Molecular Systems, Inc. | Control nucleic acids for multiple parameters |
| CN103154269A (zh) | 2010-07-29 | 2013-06-12 | 霍夫曼-拉罗奇有限公司 | 用于多种参数的对照核酸 |
| US9725754B2 (en) | 2010-07-29 | 2017-08-08 | Sean F. Boyle | Generic sample preparation |
| US9175332B2 (en) | 2010-07-29 | 2015-11-03 | Roche Molecular Systems, Inc. | Generic PCR |
| ES2605303T3 (es) | 2010-07-29 | 2017-03-13 | F. Hoffmann-La Roche Ag | Detección cualitativa y cuantitativa de ácidos nucleicos microbianos |
| CN103025891A (zh) | 2010-07-29 | 2013-04-03 | 霍夫曼-拉罗奇有限公司 | 通用样品制备 |
| EP2426222A1 (fr) | 2010-09-07 | 2012-03-07 | F. Hoffmann-La Roche AG | Tampon générique pour amplification |
| NZ610129A (en) | 2010-10-04 | 2014-08-29 | Genapsys Inc | Systems and methods for automated reusable parallel biological reactions |
| US9399217B2 (en) | 2010-10-04 | 2016-07-26 | Genapsys, Inc. | Chamber free nanoreactor system |
| US9184099B2 (en) | 2010-10-04 | 2015-11-10 | The Board Of Trustees Of The Leland Stanford Junior University | Biosensor devices, systems and methods therefor |
| EP2465945A1 (fr) | 2010-12-17 | 2012-06-20 | F. Hoffmann-La Roche AG | Matrice générique pour acides nucléiques de contrôle |
| US8585973B2 (en) | 2011-05-27 | 2013-11-19 | The Board Of Trustees Of The Leland Stanford Junior University | Nano-sensor array |
| US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
| EP2535712A1 (fr) | 2011-06-15 | 2012-12-19 | F. Hoffmann-La Roche AG | Système analytique pour la préparation d'un matériau biologique |
| GB201113698D0 (en) * | 2011-08-09 | 2011-09-21 | Wirtz Ralf M | Matrix and method for purifying and/or isolating nucleic acids |
| US20130071946A1 (en) | 2011-09-21 | 2013-03-21 | Roche Molecular Systems, Inc. | Suspension Container For Binding Particles For The Isolation Of Biological Material |
| US10093975B2 (en) | 2011-12-01 | 2018-10-09 | Genapsys, Inc. | Systems and methods for high efficiency electronic sequencing and detection |
| WO2013185078A1 (fr) * | 2012-06-07 | 2013-12-12 | Somalogic, Inc. | Essais multiplexés à base d'aptamères |
| US9399986B2 (en) | 2012-07-31 | 2016-07-26 | General Electric Company | Devices and systems for isolating biomolecules and associated methods thereof |
| US20140322706A1 (en) | 2012-10-24 | 2014-10-30 | Jon Faiz Kayyem | Integrated multipelx target analysis |
| JP1628115S (fr) | 2012-10-24 | 2019-04-01 | ||
| RU2527047C1 (ru) * | 2013-02-14 | 2014-08-27 | Виталий Богданович Черногиль | Способ изготовления микрошариков и микросфер |
| RU2527427C1 (ru) * | 2013-02-14 | 2014-08-27 | Виталий Богданович Черногиль | Способ производства микрошариков и микросфер |
| US20140272971A1 (en) * | 2013-03-14 | 2014-09-18 | Health Diagnostic Laboratory, Inc. | Unheated extraction of genomic dna in an automated laboratory system |
| CN107866286A (zh) | 2013-03-15 | 2018-04-03 | 金马克诊断股份有限公司 | 用于操纵可变形流体容器的系统、方法和设备 |
| EP2971141B1 (fr) | 2013-03-15 | 2018-11-28 | Genapsys, Inc. | Systèmes pour l'analyse biologique |
| US9409148B2 (en) | 2013-08-08 | 2016-08-09 | Uchicago Argonne, Llc | Compositions and methods for direct capture of organic materials from process streams |
| RU2540312C1 (ru) * | 2013-10-22 | 2015-02-10 | Федеральное государственное бюджетное учреждение науки Институт химии и химической технологии Сибирского отделения Российской академии наук | Способ получения магнитного аффинного сорбента для выделения рекомбинантных белков |
| US9498778B2 (en) | 2014-11-11 | 2016-11-22 | Genmark Diagnostics, Inc. | Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system |
| USD881409S1 (en) | 2013-10-24 | 2020-04-14 | Genmark Diagnostics, Inc. | Biochip cartridge |
| CN105916962B (zh) | 2013-11-19 | 2019-02-05 | 三星电子株式会社 | 发光颗粒、包含其的材料和产品、以及方法 |
| WO2015089238A1 (fr) | 2013-12-11 | 2015-06-18 | Genapsys, Inc. | Systèmes et procédés d'analyse et d'informatique biologiques |
| US9822401B2 (en) | 2014-04-18 | 2017-11-21 | Genapsys, Inc. | Methods and systems for nucleic acid amplification |
| WO2016065218A1 (fr) | 2014-10-23 | 2016-04-28 | Corning Incorporated | Nanoparticules magnétiques encapsulées par polymère |
| US10005080B2 (en) | 2014-11-11 | 2018-06-26 | Genmark Diagnostics, Inc. | Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation |
| US9598722B2 (en) | 2014-11-11 | 2017-03-21 | Genmark Diagnostics, Inc. | Cartridge for performing assays in a closed sample preparation and reaction system |
| WO2018017884A1 (fr) | 2016-07-20 | 2018-01-25 | Genapsys, Inc. | Systèmes et procédés de séquençage d'acides nucléiques |
| EP3299471B1 (fr) | 2016-09-23 | 2019-10-23 | Roche Diagniostics GmbH | Procédés pour déterminer la quantité d'acide nucléique utile dans un échantillon non traité |
| WO2019060628A1 (fr) | 2017-09-21 | 2019-03-28 | Genapsys, Inc. | Systèmes et procédés de séquençage d'acides nucléiques |
| SG11202109801YA (en) * | 2019-03-19 | 2021-10-28 | Amgen Inc | Alternate detergents for viral inactivation |
| WO2021054209A1 (fr) * | 2019-09-19 | 2021-03-25 | 三洋化成工業株式会社 | Composition de particules magnétiques, utilisation de la composition de particules magnétiques pour la séparation d'acides nucléiques, trousse pour obtention d'une composition de particules magnétiques, particules magnétiques, sel chaotropique et procédé de séparation et de purification |
| WO2021198289A1 (fr) | 2020-03-30 | 2021-10-07 | Université de Liège | Préparation de particules à noyau et enveloppe magnétiques |
| EP4015074A1 (fr) | 2020-12-17 | 2022-06-22 | Université de Liège | Préparation de particules c ur-écorce magnétiques |
| WO2022060732A1 (fr) | 2020-09-17 | 2022-03-24 | Nanophase Technologies Corporation | Billes magnétiques, procédé de fabrication et procédé d'utilisation associés |
Family Cites Families (112)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2913419A (en) * | 1956-04-18 | 1959-11-17 | Du Pont | Chemical process and composition |
| US2885366A (en) * | 1956-06-28 | 1959-05-05 | Du Pont | Product comprising a skin of dense, hydrated amorphous silica bound upon a core of another solid material and process of making same |
| US3290122A (en) * | 1964-07-28 | 1966-12-06 | Sam D Clinton | Process for preparing oxide gel microspheres from sols |
| DE2313331C2 (de) * | 1973-03-17 | 1986-11-13 | Merck Patent Gmbh, 6100 Darmstadt | Eisenoxidhaltige Glimmerschuppenpigmente |
| US3945862A (en) * | 1973-06-26 | 1976-03-23 | Merck & Co., Inc. | Coated ferrous substrates comprising an amorphous magnesia-silica complex |
| DE2625106C2 (de) * | 1976-06-04 | 1982-03-11 | Bayer Ag, 5090 Leverkusen | Eisenoxidschwarz-Pigmente mit verbesserter Oxidationsbeständigkeit und Verfahren zu ihrer Herstellung |
| US4126437A (en) * | 1976-12-02 | 1978-11-21 | Xerox Corporation | Magnetic glass carrier materials |
| US4124385A (en) * | 1976-12-02 | 1978-11-07 | Xerox Corporation | Magnetic glass carrier materials |
| US4124735A (en) * | 1976-12-02 | 1978-11-07 | Xerox Corporation | Magnetic glass carrier materials |
| US4233169A (en) * | 1979-04-13 | 1980-11-11 | Corning Glass Works | Porous magnetic glass structure |
| US4395271A (en) * | 1979-04-13 | 1983-07-26 | Corning Glass Works | Method for making porous magnetic glass and crystal-containing structures |
| US4297337A (en) * | 1979-04-13 | 1981-10-27 | Corning Glass Works | Solid-phase immunoassays using magnetic glass |
| JPS5676510A (en) * | 1979-11-28 | 1981-06-24 | Tdk Corp | Manufacture of magnetic recording material |
| JPS56105337A (en) * | 1980-01-28 | 1981-08-21 | Tdk Corp | Magnetic recording medium and its production |
| US4280918A (en) * | 1980-03-10 | 1981-07-28 | International Business Machines Corporation | Magnetic particle dispersions |
| US4360441A (en) * | 1981-06-25 | 1982-11-23 | Corning Glass Works | Glass-encapsulated magnetic materials and methods for making them |
| US4448884A (en) | 1982-03-03 | 1984-05-15 | Kms Fusion, Inc. | Glass-surface microcarrier for growth of cell cultures |
| DE3211309A1 (de) * | 1982-03-26 | 1983-09-29 | Metin Dipl.-Ing. 6100 Darmstadt Colpan | Chromatographisches verfahren zur isolierung von makromolekuelen |
| US4477492A (en) * | 1983-04-22 | 1984-10-16 | E. I. Du Pont De Nemours And Company | Process for preparing superficially porous supports for chromatography and catalysts |
| US4672040A (en) * | 1983-05-12 | 1987-06-09 | Advanced Magnetics, Inc. | Magnetic particles for use in separations |
| US4628037A (en) * | 1983-05-12 | 1986-12-09 | Advanced Magnetics, Inc. | Binding assays employing magnetic particles |
| US4554088A (en) * | 1983-05-12 | 1985-11-19 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
| US4698302A (en) * | 1983-05-12 | 1987-10-06 | Advanced Magnetics, Inc. | Enzymatic reactions using magnetic particles |
| US4695392A (en) * | 1983-05-12 | 1987-09-22 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
| US4695393A (en) * | 1983-05-12 | 1987-09-22 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
| GB8401636D0 (en) * | 1984-01-21 | 1984-02-22 | British Petroleum Co Plc | Coating process |
| US5236623A (en) * | 1984-07-11 | 1993-08-17 | Rhone-Poulenc Chimie | Process for the production of a silica colloid |
| US4824712A (en) * | 1984-07-16 | 1989-04-25 | Ppg Industries, Inc. | Treatment of glass to reduce venting during thermal treatment and a glass article made thereby |
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5512332A (en) * | 1985-10-04 | 1996-04-30 | Immunivest Corporation | Process of making resuspendable coated magnetic particles |
| US5597531A (en) * | 1985-10-04 | 1997-01-28 | Immunivest Corporation | Resuspendable coated magnetic particles and stable magnetic particle suspensions |
| US5076950A (en) * | 1985-12-20 | 1991-12-31 | Syntex (U.S.A.) Inc. | Magnetic composition for particle separation |
| US5206568A (en) * | 1986-03-26 | 1993-04-27 | Beckman Instruments, Inc. | Coordinated control of stepper motors |
| US4751211A (en) * | 1986-08-07 | 1988-06-14 | Aluminum Company Of America | Composite adsorbent for removing acids from organophosphate functional fluids |
| JPS63109105A (ja) * | 1986-10-25 | 1988-05-13 | Chisso Corp | 強磁性金属微粒子の製造方法 |
| DE3639949A1 (de) * | 1986-11-22 | 1988-06-09 | Diagen Inst Molekularbio | Verfahren zur trennung von langkettigen nukleinsaeuren |
| US4767670A (en) * | 1987-01-21 | 1988-08-30 | E. I. Du Pont De Nemours And Company | Chromatographic supports for separation of oligonucleotides |
| NO162946C (no) * | 1987-08-21 | 1990-03-14 | Otto Soerensen | Anordning for magnetisk separasjon av celler. |
| DE3724442A1 (de) | 1987-07-23 | 1989-02-02 | Europ Lab Molekularbiolog | Verfahren und vorrichtung zur reinigung von m-13-phagen-dna |
| ES2066851T3 (es) * | 1988-05-24 | 1995-03-16 | Anagen Uk Ltd | Particulas atraibles magneticamente y metodo de preparacion. |
| US5312485A (en) * | 1988-08-05 | 1994-05-17 | J. M. Huber Corporation | Precipitated encapsulated paper pigments and methods |
| US5041390A (en) * | 1988-08-11 | 1991-08-20 | Skov Per S | Method and means for allergy diagnosis |
| US5075430A (en) * | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
| US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5352645A (en) * | 1989-04-14 | 1994-10-04 | E. I. Du Pont De Nemours And Company | Silica microspheres, method of improving attrition resistance and use |
| JP2545282B2 (ja) * | 1989-04-17 | 1996-10-16 | 日東化学工業株式会社 | 球状シリカ粒子の製造方法 |
| US5055194A (en) * | 1989-07-28 | 1991-10-08 | University Of Pennsylvania | Support for high performance liquid chromatography in a magnetically stabilized fluidized bed |
| US5698271A (en) * | 1989-08-22 | 1997-12-16 | Immunivest Corporation | Methods for the manufacture of magnetically responsive particles |
| US5279936A (en) * | 1989-12-22 | 1994-01-18 | Syntex (U.S.A.) Inc. | Method of separation employing magnetic particles and second medium |
| US5523231A (en) * | 1990-02-13 | 1996-06-04 | Amersham International Plc | Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules |
| EP0443840B1 (fr) * | 1990-02-21 | 1995-04-19 | Toda Kogyo Corp. | Particules fines super-paramagnétiques d'oxyde de fer et milieux d'enregistrement magnétique utilisant de telles particules |
| US5316699A (en) * | 1990-03-28 | 1994-05-31 | The United States Of America As Repesented By The Secretary Of Commerce | Process for the controlled preparation of a composite of ultrafine magnetic particles homogeneously dispersed in a dielectric matrix |
| ATE154981T1 (de) * | 1990-04-06 | 1997-07-15 | Perkin Elmer Corp | Automatisiertes labor für molekularbiologie |
| US5693502A (en) * | 1990-06-11 | 1997-12-02 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand inhibitors to DNA polymerases |
| US5763173A (en) * | 1990-06-11 | 1998-06-09 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand inhibitors to DNA polymerases |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5217804A (en) * | 1990-11-06 | 1993-06-08 | Eastman Kodak Company | Magnetic particles |
| US5155018A (en) * | 1991-07-10 | 1992-10-13 | Hahnemann University | Process and kit for isolating and purifying RNA from biological sources |
| US5395498A (en) * | 1991-11-06 | 1995-03-07 | Gombinsky; Moshe | Method for separating biological macromolecules and means therfor |
| US5734020A (en) * | 1991-11-20 | 1998-03-31 | Cpg, Inc. | Production and use of magnetic porous inorganic materials |
| US5610274A (en) * | 1991-11-20 | 1997-03-11 | Cpg, Inc. | Production and use of magnetic porous inorganic materials |
| US5346994A (en) * | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
| DE69324716T2 (de) * | 1992-02-13 | 1999-09-09 | Becton Dickinson And Co. | Celithydrat und Reinigung von DNS |
| EP0566790B1 (fr) * | 1992-04-23 | 1996-08-07 | Toda Kogyo Corp. | Poudre magnétique et toner magnétique |
| TW221381B (fr) * | 1992-04-28 | 1994-03-01 | Du Pont | |
| IT1256064B (it) * | 1992-07-28 | 1995-11-27 | Donegani Guido Ist | Metodo per la preparazione di geli porosi contenenti boro |
| CA2107524C (fr) * | 1992-10-06 | 1999-01-19 | Hiromitsu Misawa | Particules d'oxyde de fer et methode pour les preparer |
| US5578238A (en) * | 1992-10-30 | 1996-11-26 | Lord Corporation | Magnetorheological materials utilizing surface-modified particles |
| CA2102264C (fr) * | 1992-11-13 | 2000-08-01 | Daniel Lee Woodard | Borosilicates, aluminosilicates, phosphosilicates et purification de l'adn |
| DE4307262A1 (de) | 1993-03-02 | 1994-09-08 | Christian Bergemann | Magnetisches polymeres Siliciumdioxid |
| US5648170A (en) * | 1993-04-27 | 1997-07-15 | Toda Kogyo Corporation | Coated granular magnetite particles and process for producing the same |
| AU7375794A (en) | 1993-07-28 | 1995-02-28 | Akzo Nobel N.V. | Process for isolating nucleic acid from gram positive microorganisms |
| AU689815B2 (en) * | 1993-08-30 | 1998-04-09 | Promega Corporation | Nucleic acid purification compositions and methods |
| US5468427A (en) * | 1993-09-27 | 1995-11-21 | Alfred University | Process for making ultra-fine ceramic particles |
| US5438127A (en) * | 1993-09-27 | 1995-08-01 | Becton Dickinson And Company | DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane |
| US5503816A (en) * | 1993-09-27 | 1996-04-02 | Becton Dickinson And Company | Silicate compounds for DNA purification |
| US5523065A (en) * | 1993-09-27 | 1996-06-04 | Alfred University | Process for making ultra-fine barium titanate particles |
| US5599627A (en) | 1993-10-08 | 1997-02-04 | Toda Kogyo Corporation | Magnetic particles comprising magnetite core and process for producing the same |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5990301A (en) * | 1994-02-07 | 1999-11-23 | Qiagen Gmbh | Process for the separation and purification of nucleic acids from biological sources |
| ATE187733T1 (de) * | 1994-02-07 | 2000-01-15 | Qiagen Gmbh | Verfahren zur herstellung endotoxinfreier oder an endotoxin abgereicherter nucleinsäuren und/oder oligonucleotide für die gentherapie |
| GB9411572D0 (en) * | 1994-06-09 | 1994-08-03 | Amersham Int Plc | Magnetic bead precipitation method |
| ATE191086T1 (de) * | 1994-07-27 | 2000-04-15 | Pilgrimm Herbert | Superparamagnetische teilchen, verfahren zur herstellung und deren verwendung |
| US5817327A (en) * | 1994-07-27 | 1998-10-06 | The Trustees Of The University Of Pennsylvania | Incorporation of biologically active molecules into bioactive glasses |
| US5582988A (en) * | 1994-09-15 | 1996-12-10 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same |
| US5705628A (en) * | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US5660984A (en) * | 1994-12-09 | 1997-08-26 | Davis; Thomas E. | DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof |
| FR2732116B1 (fr) * | 1995-03-21 | 1997-05-09 | Bio Merieux | Procede et dispositif pour la determination qualitative et/ou quantitative d'un analyte, notamment d'une bacterie, dans un echantillon, par voie magnetique |
| US5683875A (en) * | 1995-05-04 | 1997-11-04 | Hewlett-Packard Company | Method for detecting a target nucleic acid analyte in a sample |
| IN188702B (fr) * | 1995-06-01 | 2002-10-26 | Degussa | |
| WO2000032762A1 (fr) * | 1998-11-30 | 2000-06-08 | Roche Diagnostics Gmbh | Particules magnetiques pour purifier des acides nucleiques |
| DE19520964A1 (de) * | 1995-06-08 | 1996-12-12 | Inst Neue Mat Gemein Gmbh | Beschichtete anorganische Pigmente, Verfahren zu deren Herstellung und deren Verwendung |
| DE19854973B4 (de) * | 1998-11-30 | 2010-02-04 | Institut Für Neue Materialien Gem. Gmbh | Verfahren zur Reinigung von Nukleinsäuren |
| KR100463475B1 (ko) * | 1995-06-08 | 2005-06-22 | 로셰 디아그노스틱스 게엠베하 | 자기성피그먼트 |
| DE19520398B4 (de) * | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| JP2965131B2 (ja) * | 1995-07-07 | 1999-10-18 | 東洋紡績株式会社 | 核酸結合用磁性担体およびそれを用いる核酸単離方法 |
| US5578179A (en) * | 1995-07-12 | 1996-11-26 | The Perkin-Elmer Corporation | Method and silicate composition for conditioning silica surfaces |
| US5783686A (en) * | 1995-09-15 | 1998-07-21 | Beckman Instruments, Inc. | Method for purifying nucleic acids from heterogenous mixtures |
| US5904848A (en) * | 1996-02-21 | 1999-05-18 | Cpg, Inc. | Controlled pore glass-synthetic resin membrane |
| US5972721A (en) * | 1996-03-14 | 1999-10-26 | The United States Of America As Represented By The Secretary Of The Air Force | Immunomagnetic assay system for clinical diagnosis and other purposes |
| DE19622885A1 (de) | 1996-06-07 | 1997-12-11 | Boehringer Mannheim Gmbh | Reagenzzubereitung enthaltend magnetische Partikel in Form einer Tablette |
| US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
| PL191422B1 (pl) * | 1997-01-21 | 2006-05-31 | Grace W R & Co | Adsorbent o cząstkach stałych i sposób wytwarzania cząstek adsorbenta |
| ATE280177T1 (de) | 1997-03-20 | 2004-11-15 | Hoffmann La Roche | Modifizierte primer |
| DE19743518A1 (de) * | 1997-10-01 | 1999-04-15 | Roche Diagnostics Gmbh | Automatisierbare universell anwendbare Probenvorbereitungsmethode |
| US5990479A (en) * | 1997-11-25 | 1999-11-23 | Regents Of The University Of California | Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes |
| DE69815282T2 (de) | 1997-12-25 | 2004-05-06 | Tosoh Corp., Shinnanyo | Magnetischer Träger, seine Herstellung, und Verfahren zum Extrahieren von Nukleinsäuren |
| PT1232502E (pt) | 1999-11-17 | 2006-05-31 | Roche Diagnostics Gmbh | Particulas de vidro magneticas, metodo para a sua preparacao e suas utilizacoes |
| US7267964B2 (en) * | 2000-03-31 | 2007-09-11 | Merial Limited | DNA molecules encoding ligand gated ion channels from Dermacentor variabilis |
| US20030032146A1 (en) | 2001-07-10 | 2003-02-13 | Dwulet Francis Edward | Enzyme/tag binding and detection system |
-
2000
- 2000-11-17 PT PT00981267T patent/PT1232502E/pt unknown
- 2000-11-17 SK SK664-2002A patent/SK287932B6/sk not_active IP Right Cessation
- 2000-11-17 AT AT00981267T patent/ATE315826T1/de active
- 2000-11-17 DK DK00981267T patent/DK1232502T3/da active
- 2000-11-17 CA CA002392009A patent/CA2392009C/fr not_active Expired - Lifetime
- 2000-11-17 DE DE60025529T patent/DE60025529T2/de not_active Expired - Lifetime
- 2000-11-17 ES ES00981267T patent/ES2256068T3/es not_active Expired - Lifetime
- 2000-11-17 HU HU0203386A patent/HUP0203386A3/hu not_active Application Discontinuation
- 2000-11-17 JP JP2001537749A patent/JP2003514383A/ja active Pending
- 2000-11-17 WO PCT/EP2000/011459 patent/WO2001037291A1/fr not_active Ceased
- 2000-11-17 CZ CZ20021608A patent/CZ20021608A3/cs unknown
- 2000-11-17 PL PL356771A patent/PL202358B1/pl not_active IP Right Cessation
- 2000-11-17 EP EP00981267A patent/EP1232502B1/fr not_active Expired - Lifetime
-
2002
- 2002-05-16 US US10/147,679 patent/US20030224366A1/en not_active Abandoned
-
2005
- 2005-05-11 US US11/128,024 patent/US8129118B2/en not_active Expired - Fee Related
-
2006
- 2006-01-27 JP JP2006019516A patent/JP4361905B2/ja not_active Expired - Lifetime
- 2006-04-03 CY CY20061100461T patent/CY1105229T1/el unknown
-
2012
- 2012-02-13 US US13/372,218 patent/US20120247150A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA2392009C (fr) | 2009-07-14 |
| EP1232502B1 (fr) | 2006-01-11 |
| CA2392009A1 (fr) | 2001-05-25 |
| DK1232502T3 (da) | 2006-05-22 |
| US20120247150A1 (en) | 2012-10-04 |
| JP2003514383A (ja) | 2003-04-15 |
| HUP0203386A1 (hu) | 2002-12-28 |
| JP4361905B2 (ja) | 2009-11-11 |
| DE60025529T2 (de) | 2006-08-24 |
| WO2001037291A1 (fr) | 2001-05-25 |
| JP2006193421A (ja) | 2006-07-27 |
| US8129118B2 (en) | 2012-03-06 |
| HUP0203386A3 (en) | 2010-01-28 |
| CY1105229T1 (el) | 2010-03-03 |
| DE60025529D1 (de) | 2006-04-06 |
| US20030224366A1 (en) | 2003-12-04 |
| ES2256068T3 (es) | 2006-07-16 |
| PT1232502E (pt) | 2006-05-31 |
| CZ20021608A3 (cs) | 2003-06-18 |
| US20050266462A1 (en) | 2005-12-01 |
| ATE315826T1 (de) | 2006-02-15 |
| SK6642002A3 (en) | 2003-08-05 |
| PL202358B1 (pl) | 2009-06-30 |
| PL356771A1 (en) | 2004-07-12 |
| EP1232502A1 (fr) | 2002-08-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SK287932B6 (sk) | Magnetic glass particles, method for their preparation and uses thereof | |
| JP4148900B2 (ja) | 増幅反応におけるシリカ物質の使用 | |
| JP4048022B2 (ja) | 自動化可能なユニバーサル試料調製方法 | |
| US9868756B2 (en) | Method for bisulfite treatment | |
| JP4456046B2 (ja) | 磁性色素 | |
| CN101082062B (zh) | 单个细胞的基因表达定量方法 | |
| JP5537894B2 (ja) | 磁性色素 | |
| WO2007103558A2 (fr) | Transfert d'energie hybride pour la detection d'acide nucleique | |
| CA2614069A1 (fr) | Isolement d'acide nucleique au moyen de polidocanol et de derives connexes | |
| EP1932913A1 (fr) | Isolation d'acide nucléique utilisant du polidocanol et des dérivés | |
| KR102738301B1 (ko) | 다중 진단을 위한 핵산 분리 및 검출용 미세입자 프로브 | |
| EP1154443A1 (fr) | Particules magnétiques de verre, méthode de fabrication et utilisation | |
| KR102880002B1 (ko) | 단일 염기 다형성 분석을 위한 미세입자 프로브 | |
| KR20230066585A (ko) | 자성 비드, 이의 제조 방법 및 사용 방법 | |
| JP2001078761A (ja) | 核酸結合性磁性シリカ粒子担体 | |
| EP4527943A1 (fr) | Sonde de diagnostic à microparticules comprenant une particule magnétique et des ciseaux génétiques inactivés, ainsi que système et procédé multi-diagnostic impliquant la sonde |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Patent lapsed due to non-payment of maintenance fees |
Effective date: 20121117 |