SK7702000A3 - Process for preparing coryneform bacteria having an improved amino acid- or nucleic acid-productivity - Google Patents

Process for preparing coryneform bacteria having an improved amino acid- or nucleic acid-productivity Download PDF

Info

Publication number: SK7702000A3
Authority: SK; Slovakia
Prior art keywords: promoter; sequence; region; gene; amino acid
Prior art date: 1998-09-25

Application number

SK770-2000A

Other languages

English (en)

Slovak (sk)

Inventor

Yoko Asakura

Jun Nakamura

Sohei Kanno

Mikiko Suga

Eiichiro Kimura

Hisao Ito

Kazuhiko Matsui

Tsuyoshi Ohsumi

Tsuyoshi Nakamatsu

Osamu Kurahashi

Original Assignee

Ajinomoto Kk

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-09-25

Filing date

1999-09-22

Publication date

2000-10-09

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26549879&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SK7702000(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1999-09-22 Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk

2000-10-09 Publication of SK7702000A3 publication Critical patent/SK7702000A3/sk

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/14—Glutamic acid; Glutamine
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression

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Genetics & Genomics (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Chemical & Material Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Molecular Biology (AREA)
Plant Pathology (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

SK770-2000A 1998-09-25 1999-09-22 Process for preparing coryneform bacteria having an improved amino acid- or nucleic acid-productivity SK7702000A3 (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
JP27178798		1998-09-25
JP27178698		1998-09-25
PCT/JP1999/005175 WO2000018935A1 (en)	1998-09-25	1999-09-22	Process for constructing amino acid-producing bacterium and process for producing amino acid by fermentation method with the use of the thus constructed amino acid-producing bacterium

Publications (1)

Publication Number	Publication Date
SK7702000A3 true SK7702000A3 (en)	2000-10-09

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ID=26549879

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Application Number	Title	Priority Date	Filing Date
SK770-2000A SK7702000A3 (en)	1998-09-25	1999-09-22	Process for preparing coryneform bacteria having an improved amino acid- or nucleic acid-productivity

Country Status (16)

Country	Link
US (2)	US6962805B2 (de)
EP (1)	EP1033407B1 (de)
KR (1)	KR100630604B1 (de)
CN (1)	CN1170938C (de)
AT (1)	ATE447039T1 (de)
BR (2)	BR9917880B1 (de)
DE (1)	DE69941594D1 (de)
ES (1)	ES2335828T3 (de)
HU (2)	HU224975B1 (de)
ID (1)	ID25513A (de)
MY (1)	MY130756A (de)
PE (1)	PE20001111A1 (de)
RU (1)	RU2214456C2 (de)
SK (1)	SK7702000A3 (de)
TW (4)	TWI247805B (de)
WO (1)	WO2000018935A1 (de)

Families Citing this family (151)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN1165619C (zh)	1995-06-07	2004-09-08	味之素株式会社	制备l-赖氨酸的方法
SK7702000A3 (en)	1998-09-25	2000-10-09	Ajinomoto Kk	Process for preparing coryneform bacteria having an improved amino acid- or nucleic acid-productivity
WO2002008437A2 (en) *	2000-07-24	2002-01-31	Degussa Ag	Process for the fermentative preparation of l-glutamic acid using coryneform bacteria
DE60133304T2 (de) *	2000-11-17	2009-03-05	Cj Cheiljedang Corp.	L-glutamine produzierende mikroorganismen und verfahren zur herstellung von l-glutamine unter verwendung derselben
BR0304860A (pt) *	2002-11-11	2004-08-31	Ajinomoto Kk	Método para produzir uma substância alvo pela utilização de uma-bactéria pertencente ao gênero escherichia
DE60335774D1 (de) *	2002-11-26	2011-03-03	Ajinomoto Kk	Methode für die Produktion von L-Glutamin und L-Glutamin-produzierendes Bakterium
RU2268305C2 (ru) *	2003-12-18	2006-01-20	Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ)	Способ получения l-аминокислот с использованием бактерий с оптимизированным уровнем генной экспрессии
DE10359594A1 (de) *	2003-12-18	2005-07-28	Basf Ag	PEF-TU-Expressionseinheiten
BRPI0506369B1 (pt) *	2004-01-30	2020-11-03	Ajinomoto Co., Inc	microorganismo transgênico, e, método para produzir um l-aminoácido
BRPI0509056A (pt)	2004-03-31	2007-08-21	Ajinomoto Kk	bactéria pertencendo ao gênero bacillus ou ao gênero escherichia, e, métodos para produzir um nucleosìdeo de purina, para produzir um nucleotìdeo de purina e para produzir ácido 5'-guanìlico
DE102004035065A1 (de)	2004-07-20	2006-02-16	Basf Ag	P-ET-TS-Expressionseinheiten
JP2006063002A (ja) *	2004-08-25	2006-03-09	Ajinomoto Co Inc	反芻動物用のメタン生成抑制剤および飼料用組成物
CN104073506B (zh) *	2004-10-13	2018-02-13	三井化学株式会社	编码具有d‑丝氨酸合成活性的酶的dna、该酶的制备方法及d‑丝氨酸制备方法
JP4595506B2 (ja) *	2004-11-25	2010-12-08	味の素株式会社	Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造方法
KR100620092B1 (ko) *	2004-12-16	2006-09-08	씨제이 주식회사	코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
BR122019024048B1 (pt)	2004-12-28	2022-05-24	Ajinomoto Co., Inc	Método para produzir ácido l-glutâmico
US7794989B2 (en) *	2004-12-28	2010-09-14	Ajinomoto Co., Inc.	L-glutamic acid-producing microorganism and a method for producing L-glutamic acid
US7326546B2 (en)	2005-03-10	2008-02-05	Ajinomoto Co., Inc.	Purine-derived substance-producing bacterium and a method for producing purine-derived substance
US7781191B2 (en) *	2005-04-12	2010-08-24	E. I. Du Pont De Nemours And Company	Treatment of biomass to obtain a target chemical
CA2603774C (en) *	2005-04-12	2015-11-17	E.I. Du Pont De Nemours And Company	System and process for biomass treatment
US20070029252A1 (en) *	2005-04-12	2007-02-08	Dunson James B Jr	System and process for biomass treatment
TWI361834B (en)	2005-04-12	2012-04-11	Kyowa Hakko Bio Co Ltd	A method for producing amino acids
JP2008283863A (ja) *	2005-08-26	2008-11-27	Ajinomoto Co Inc	Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造方法
JP5088136B2 (ja)	2005-10-18	2012-12-05	味の素株式会社	コハク酸の製造方法
BRPI0709635A2 (pt)	2006-04-24	2011-07-19	Ajinomoto Kk	bactéria pertencendo ao gênero bacillus, e, métodos para produzir uma substáncia derivada de purina, e um nucleotìdeo de purina
ES2401607T3 (es)	2006-04-24	2013-04-23	Ajinomoto Co., Inc.	Bacteria que puede producir una sustancia de purina y procedimiento para producir una sustancia de purina
WO2007136133A1 (en)	2006-05-23	2007-11-29	Ajinomoto Co., Inc.	A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family
WO2008044614A1 (en)	2006-09-28	2008-04-17	Ajinomoto Co., Inc.	Method for producing 4-hydroxy-l-isoleucine
KR100822041B1 (ko)	2006-12-21	2008-04-15	씨제이제일제당 (주)	몰리브덴 보조인자 생합성 효소 에이 코딩 유전자의 발현이강화된 코리네박테리움 속 미생물 및 이를 이용한엘-라이신 생산방법
KR100882418B1 (ko) *	2007-01-15	2009-02-05	씨제이제일제당 (주)	이노신을 생산하는 미생물 및 그를 이용한 이노신 제조방법
JP5540504B2 (ja)	2007-01-22	2014-07-02	味の素株式会社	Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
JP2010088301A (ja) *	2007-02-01	2010-04-22	Ajinomoto Co Inc	Ｌ−アミノ酸の製造法
JP2010110217A (ja) *	2007-02-22	2010-05-20	Ajinomoto Co Inc	Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造法
JP2010130899A (ja)	2007-03-14	2010-06-17	Ajinomoto Co Inc	Ｌ−グルタミン酸系アミノ酸生産微生物及びアミノ酸の製造法
WO2008133131A1 (ja)	2007-04-16	2008-11-06	Ajinomoto Co., Inc.	有機酸の製造方法
CN101688176B (zh)	2007-04-17	2013-11-06	味之素株式会社	具有羧基的酸性物质的生产方法
US7807419B2 (en) *	2007-08-22	2010-10-05	E. I. Du Pont De Nemours And Company	Process for concentrated biomass saccharification
US8445236B2 (en) *	2007-08-22	2013-05-21	Alliance For Sustainable Energy Llc	Biomass pretreatment
US7819976B2 (en) *	2007-08-22	2010-10-26	E. I. Du Pont De Nemours And Company	Biomass treatment method
US20090053800A1 (en) *	2007-08-22	2009-02-26	Julie Friend	Biomass Treatment Apparatus
JP2010263790A (ja) *	2007-09-04	2010-11-25	Ajinomoto Co Inc	アミノ酸生産微生物及びアミノ酸の製造法
RU2396336C2 (ru)	2007-09-27	2010-08-10	Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ)	СПОСОБ ПОЛУЧЕНИЯ АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia
WO2009072562A1 (ja)	2007-12-06	2009-06-11	Ajinomoto Co., Inc.	有機酸の製造方法
JP2011067095A (ja)	2008-01-10	2011-04-07	Ajinomoto Co Inc	発酵法による目的物質の製造法
WO2009093703A1 (ja)	2008-01-23	2009-07-30	Ajinomoto Co., Inc.	Ｌ-アミノ酸の製造法
WO2009154280A1 (ja) *	2008-06-19	2009-12-23	味の素株式会社	過食防止剤
JP5488467B2 (ja)	2008-09-05	2014-05-14	味の素株式会社	Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造法
EP2336347B1 (de)	2008-09-08	2017-03-15	Ajinomoto Co., Inc.	L-aminosäure-produzierender mikroorganismus und verfahren zur herstellung von l-aminosäure
JP2012029565A (ja)	2008-11-27	2012-02-16	Ajinomoto Co Inc	Ｌ−アミノ酸の製造法
BRPI1014661B1 (pt)	2009-07-29	2020-12-15	Ajinomoto Co., Inc.	Método para produzir um l-aminoácido
JP5636648B2 (ja)	2009-08-10	2014-12-10	味の素株式会社	５’−グアニル酸の製造法
JP2012223091A (ja)	2009-08-25	2012-11-15	Ajinomoto Co Inc	Ｌ−アミノ酸の製造法
JP2012223092A (ja)	2009-08-28	2012-11-15	Ajinomoto Co Inc	Ｌ−アミノ酸の製造法
JP2013013329A (ja)	2009-11-06	2013-01-24	Ajinomoto Co Inc	Ｌ−アミノ酸の製造法
JP2013074795A (ja)	2010-02-08	2013-04-25	Ajinomoto Co Inc	変異型ｒｐｓＡ遺伝子及びＬ−アミノ酸の製造法
KR101226384B1 (ko)	2010-03-05	2013-01-25	씨제이제일제당 (주)	개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법
US8721794B2 (en)	2010-04-28	2014-05-13	E I Du Pont De Nemours And Company	Production of high solids syrup from lignocellulosic biomass hydrolysate fermentation broth
US8906235B2 (en)	2010-04-28	2014-12-09	E I Du Pont De Nemours And Company	Process for liquid/solid separation of lignocellulosic biomass hydrolysate fermentation broth
RU2501858C2 (ru)	2010-07-21	2013-12-20	Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ)	СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТЫ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА Enterobacteriaceae
EP2615170B1 (de)	2010-09-08	2015-11-04	Green Phenol Development Co., Ltd.	Mittel zur transformierung coryneformer bakterien sowie verfahren zur phenolherstellung damit
BR112013006031A2 (pt)	2010-09-14	2016-06-07	Ajinomoto Kk	bactéria,e, método para produzir um aminoácido contendo enxofre, uma substância relacionada ao mesmo, ou uma mnistura dos mesmos.
EP2639295B1 (de)	2010-11-10	2017-06-28	Green Phenol Development Co., Ltd.	Mittel zur transformierung coryneformer bakterien sowie verfahren zur phenolherstellung damit
JP2014036576A (ja)	2010-12-10	2014-02-27	Ajinomoto Co Inc	Ｌ−アミノ酸の製造法
JP2014087259A (ja)	2011-02-22	2014-05-15	Ajinomoto Co Inc	Ｌ−システイン生産菌及びｌ−システインの製造法
EP2687591B1 (de)	2011-03-18	2021-01-06	Mitsubishi Chemical Corporation	Verfahren zur herstellung von polymeren, herstellungsverfahren für organische säuren und organische säuren produzierender mikroorganismus
EP2695940B1 (de)	2011-04-01	2016-11-30	Ajinomoto Co., Inc.	Verfahren zur herstellung von l-cystein
DE102011006716A1 (de)	2011-04-04	2012-10-04	Evonik Degussa Gmbh	Mikroorganismus und Verfahren zur fermentativen Herstellung einer organisch-chemischen Verbindung
JP2014131487A (ja)	2011-04-18	2014-07-17	Ajinomoto Co Inc	Ｌ−システインの製造法
RU2496867C2 (ru)	2011-04-25	2013-10-27	Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ")	Способ получения l-аминокислоты семейства глутамата с использованием коринеформной бактерии
JP6136930B2 (ja)	2011-11-02	2017-05-31	味の素株式会社	タンパク質の分泌生産法
WO2013069634A1 (ja)	2011-11-11	2013-05-16	味の素株式会社	発酵法による目的物質の製造法
RU2012129311A (ru)	2012-07-11	2014-01-20	Закрытое акционерное общество " Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ")	Днк, кодирующая дипептид-синтезирующий фермер (варианты), бактерия рода escherichia и способ получения дипептидов с ее использованием
RU2550269C2 (ru)	2012-08-17	2015-05-10	Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ")	СПОСОБ ПОЛУЧЕНИЯ L-АРГИНИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА Enterobacteriaceae, СОДЕРЖАЩЕЙ N-АЦЕТИЛОРНИТИНДЕАЦЕТИЛАЗУ С НАРУШЕННОЙ АКТИВНОСТЬЮ
EP2762571A1 (de)	2013-01-30	2014-08-06	Evonik Industries AG	Mikroorganismus und Verfahren zur fermentativen Herstellung von Aminosäuren
JP6335196B2 (ja)	2013-02-08	2018-05-30	ニンシャーエッペンバイオテックカンパニーリミテッド	アコニターゼ遺伝子および／またはその調節エレメントの改変により、ｌ−リジンを生産する方法
US20140273105A1 (en)	2013-03-12	2014-09-18	E I Du Pont De Nemours And Company	Gradient pretreatment of lignocellulosic biomass
US20140315258A1 (en)	2013-03-14	2014-10-23	Abengoa Bioenergy New Technologies, Llc	Methods for converting cellulosic waste to bioproducts
RU2013118637A (ru)	2013-04-23	2014-10-27	Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ")	СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ РАЗРЕГУЛИРОВАН ГЕН yjjK
RU2013119826A (ru)	2013-04-29	2014-11-10	Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ")	Коринеформная бактерия и способ получения гетерологичных гибридных белков
EP2868745B1 (de)	2013-05-13	2017-06-21	Ajinomoto Co., Inc.	Verfahren zur Herstellung einer L-Aminosäure
EA201591846A1 (ru) *	2013-05-17	2016-08-31	Ксилеко, Инк.	Обработка биомассы
JP2016165225A (ja)	2013-07-09	2016-09-15	味の素株式会社	有用物質の製造方法
RU2013140115A (ru)	2013-08-30	2015-03-10	Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ")	СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ НАРУШЕНА ЭКСПРЕССИЯ КЛАСТЕРА ГЕНОВ znuACB
JP2016192903A (ja)	2013-09-17	2016-11-17	味の素株式会社	海藻由来バイオマスからのｌ−アミノ酸の製造方法
JP5958653B2 (ja)	2013-10-02	2016-08-02	味の素株式会社	アンモニア制御装置およびアンモニア制御方法
WO2015050184A1 (ja)	2013-10-02	2015-04-09	味の素株式会社	ヘパロサン生産細菌及びヘパロサンの製造法
RU2013144250A (ru)	2013-10-02	2015-04-10	Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ")	СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА, КОДИРУЮЩЕГО ФОСФАТНЫЙ ТРАНСПОРТЕР
EP2886651B1 (de)	2013-10-21	2018-08-22	Ajinomoto Co., Inc.	Verfahren zur herstellung von l-aminosäure
JP6519476B2 (ja)	2013-10-23	2019-05-29	味の素株式会社	目的物質の製造法
US10227623B2 (en)	2013-11-24	2019-03-12	E I Du Pont De Nemours And Company	High force and high stress destructuring of cellulosic biomass
JP6582997B2 (ja)	2014-01-31	2019-10-02	味の素株式会社	変異型グルタミン酸−システインリガーゼ、及び、γ−グルタミルバリルグリシンの製造法
WO2016007350A1 (en)	2014-07-09	2016-01-14	Danisco Us Inc.	Preconditioning of lignocellulosic biomass
US20170327850A1 (en) *	2014-10-30	2017-11-16	Board Of Regents, The University Of Texas System	Engineered fungi for itaconic acid production
CN104513830B (zh) *	2015-01-27	2017-08-11	华东理工大学	一种适用于氧化葡萄糖酸杆菌的基因表达载体及其应用
WO2016185211A1 (en)	2015-05-19	2016-11-24	Lucite International Uk Limited	Process for the biological productiion of methacrylic acid and derivatives thereof
RU2015120052A (ru)	2015-05-28	2016-12-20	Аджиномото Ко., Инк.	Способ получения L-аминокислоты с использованием бактерии семейства Enterobacteriaceae, в которой ослаблена экспрессия гена gshA
CN108350412B (zh)	2015-10-27	2022-02-11	味之素株式会社	用于生产醛的方法
JP6623690B2 (ja)	2015-10-30	2019-12-25	味の素株式会社	グルタミン酸系ｌ−アミノ酸の製造法
CN108463555A (zh)	2016-01-12	2018-08-28	味之素株式会社	生产苯甲醛的方法
KR102335301B1 (ko)	2016-10-21	2021-12-06	아지노모토 가부시키가이샤	단백질의 분비 생산법
WO2018079683A1 (en)	2016-10-26	2018-05-03	Ajinomoto Co., Inc.	Method for producing objective substance
WO2018079684A1 (en)	2016-10-26	2018-05-03	Ajinomoto Co., Inc.	Method for producing objective substance
GB201619827D0 (en)	2016-11-23	2017-01-04	Lucite Int Uk Ltd	Process for the production of methyl methacrylate
WO2019059404A1 (ja)	2017-09-25	2019-03-28	味の素株式会社	タンパク質の製造法および二糖の製造法
WO2019078095A1 (en)	2017-10-16	2019-04-25	Ajinomoto Co., Inc.	PROCESS FOR PRODUCTION OF PROTEIN HAVING ALPHA-HYDROXYLANTIC PEPTIDYLGLYCIN MONOOXYGENASE ACTIVITY
US11680279B2 (en)	2017-11-29	2023-06-20	Ajinomoto Co., Inc.	Method for producing objective substance
BR112020012044A2 (pt)	2017-12-26	2020-11-24	Ajinomoto Co., Inc.	método para produzir glicina ou um sal da mesma, e, bactéria produtora de glicina.
JP7367667B2 (ja)	2018-02-20	2023-10-24	味の素株式会社	Ｒｎａサイレンシングを誘導する方法
JP7124338B2 (ja)	2018-02-27	2022-08-24	味の素株式会社	変異型グルタチオン合成酵素、及び、γ－グルタミルバリルグリシンの製造法
HRP20231510T1 (hr)	2018-07-11	2024-03-01	Ajinomoto Co., Inc.	Postupak enzimskog sulfuriliranja alkohola i amina uz pomoć bakterije iz porodice enterobacteriaceae
WO2020027251A1 (en)	2018-08-03	2020-02-06	Ajinomoto Co., Inc.	Method for producing objective substance
WO2020067487A1 (en)	2018-09-28	2020-04-02	Ajinomoto Co., Inc.	Method for producing l-methionine using a bacterium
EP3861109A1 (de)	2018-10-05	2021-08-11	Ajinomoto Co., Inc.	Verfahren zur herstellung einer zielsubstanz durch bakterielle fermentation
AU2019364829B2 (en)	2018-10-25	2025-11-20	Ajinomoto Co., Inc.	Method for secretory production of protein
KR102075160B1 (ko)	2018-12-13	2020-02-10	대상 주식회사	L-글루탐산 생산능이 향상된 변이 균주 및 이를 이용한 l-글루탐산의 제조 방법
WO2020138178A1 (en)	2018-12-27	2020-07-02	Ajinomoto Co., Inc.	Method for producing basic l-amino acids or salts thereof by fermentation of an enterobacteriaceae bacterium
BR112021014194A2 (pt)	2019-02-22	2021-12-28	Ajinomoto Kk	Método para a produção de um l-aminoácido
BR112021017870A2 (pt)	2019-04-05	2021-12-07	Ajinomoto Kk	Método para produzir um l-aminoácido
JP7764852B2 (ja)	2019-09-25	2025-11-06	味の素株式会社	細菌の発酵による２－メチル酪酸の製造方法
JP7655312B2 (ja)	2019-09-25	2025-04-02	味の素株式会社	細菌の発酵によるｌ－アミノ酸の製造方法
JP7735863B2 (ja)	2019-10-28	2025-09-09	味の素株式会社	ベンズアルデヒドの製造方法
CN110951662B (zh) *	2019-12-26	2024-03-12	新疆梅花氨基酸有限责任公司	一种高产赖氨酸的棒状细菌及其构建方法与应用
KR102269639B1 (ko)	2020-02-12	2021-06-25	대상 주식회사	L-글루탐산 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-글루탐산의 생산 방법
CN111334535B (zh) *	2020-03-26	2021-11-30	廊坊梅花生物技术开发有限公司	生产l-氨基酸的重组菌株及其应用
CN113201535B (zh) *	2020-07-20	2022-02-08	中国科学院天津工业生物技术研究所	谷氨酸脱氢酶基因启动子的突变体及其应用
CN113201536B (zh) *	2020-08-19	2022-09-13	中国科学院天津工业生物技术研究所	具有启动子活性的多核苷酸及其在生产氨基酸中的应用
WO2022071061A1 (ja)	2020-09-29	2022-04-07	味の素株式会社	変異型トランスグルタミナーゼ
JP7845187B2 (ja)	2020-10-28	2026-04-14	味の素株式会社	Ｌ－アミノ酸の製造法
CN112695036B (zh) *	2021-03-23	2021-07-06	中国科学院天津工业生物技术研究所	一种天冬氨酸激酶基因表达调控序列及其应用
US20240191270A1 (en)	2021-04-16	2024-06-13	Inbiose N.V.	Fermentative production
KR20230170961A (ko)	2021-04-16	2023-12-19	인바이오스 엔.브이.	시알릴화 이당 및/또는 올리고당의 세포 생산
KR102377745B1 (ko) *	2021-05-12	2022-03-23	씨제이제일제당 주식회사	신규 프로모터 및 이의 용도
CN113308426B (zh) *	2021-05-27	2022-10-18	齐鲁工业大学	一种改造tk基因5′端序列的重组棒状杆菌及其应用
KR20240032941A (ko)	2021-07-07	2024-03-12	아지노모토 가부시키가이샤	비천연 아미노산 함유 단백질의 분비 생산법
CA3228922A1 (en)	2021-10-11	2023-04-20	Ajinomoto Co., Inc.	Bacterium modified to express heterologous tat protein
JPWO2023200008A1 (de)	2022-04-15	2023-10-19
KR102636674B1 (ko)	2022-07-11	2024-02-15	대상 주식회사	L-글루탐산을 생산하는 코리네박테리움 속 변이 미생물 및 이를 이용한 l-글루탐산의 생산 방법
KR102572849B1 (ko)	2022-07-11	2023-08-31	대상 주식회사	L-글루탐산을 생산하는 코리네박테리움 속 변이 미생물 및 이를 이용한 l-글루탐산의 생산 방법
KR102792507B1 (ko)	2022-07-11	2025-04-09	대상 주식회사	L-글루탐산을 생산하는 코리네박테리움 속 변이 미생물 및 이를 이용한 l-글루탐산의 생산 방법
KR102572850B1 (ko)	2022-07-11	2023-08-31	대상 주식회사	L-글루탐산을 생산하는 코리네박테리움 속 변이 미생물 및 이를 이용한 l-글루탐산의 생산 방법
GB2621337A (en)	2022-08-08	2024-02-14	Mitsubishi Chemical Uk Ltd	Process for the biological production of methacrylic acid and derivatives thereof
US20240117393A1 (en)	2022-09-30	2024-04-11	Ajinomoto Co., Inc.	Method for producing l-amino acid
KR102546734B1 (ko)	2022-10-31	2023-06-23	대상 주식회사	L-글루탐산을 생산하는 코리네박테리움 속 변이 미생물 및 이를 이용한 l-글루탐산의 생산 방법
KR102636672B1 (ko)	2022-10-31	2024-02-15	대상 주식회사	L-글루탐산을 생산하는 코리네박테리움 속 변이 미생물 및 이를 이용한 l-글루탐산의 생산 방법
KR102905116B1 (ko)	2022-10-31	2025-12-31	대상 주식회사	L-글루탐산을 생산하는 코리네박테리움 속 변이 미생물 및 이를 이용한 l-글루탐산의 생산 방법
KR20250151452A (ko)	2023-02-16	2025-10-21	아지노모토 가부시키가이샤	단백질의 분비 생산법
KR102843469B1 (ko)	2023-04-28	2025-08-12	대상 주식회사	L-글루탐산을 생산하는 코리네박테리움 속 미생물 및 이를 이용한 l-글루탐산 생산 방법
WO2024258202A1 (ko)	2023-06-16	2024-12-19	대상 주식회사	알킬하이드로페록시다아제 ahpd 패밀리 코어 도메인 신규 변이체 및 이를 이용한 l-글루탐산의 생산 방법
JP7716823B1 (ja)	2023-12-28	2025-08-01	味の素株式会社	Ｌ－グルタミン酸生産菌およびｌ－グルタミン酸の製造方法
EP4692312A1 (de)	2023-12-28	2026-02-11	Ajinomoto Co., Inc.	L-glutaminsäure produzierendes bakterium und verfahren zur herstellung von l-glutaminsäure
CN120505260A (zh) *	2024-02-19	2025-08-19	廊坊梅花生物技术开发有限公司	一种生产l-谷氨酸的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
LU81207A1 (fr)	1979-04-30	1980-12-16	Arbed	Procede d'affinage d'un bain de metal contenant des matieres refroidissantes solides
JPS61104790A (ja)	1984-10-29	1986-05-23	Showa Denko Kk	Ｌ−トリプトフアンの生合成に関与する遺伝情報を有する新規組換え体ｄｎａ
JP2520895B2 (ja) *	1987-03-04	1996-07-31	旭化成工業株式会社	Ｌ―グルタミン酸の製造方法
JP2708168B2 (ja)	1988-02-23	1998-02-04	昭和電工株式会社	微生物の改良
JPH06502548A (ja) *	1991-07-30	1994-03-24	オルサン	特にコリネバクテリア中で用いることのできる蛋白質の発現および分泌系
WO1995034672A1 (en) *	1994-06-14	1995-12-21	Ajinomoto Co., Inc.	α-KETOGLUTARIC DEHYDROGENASE GENE
EP2034021A1 (de)	1996-08-23	2009-03-11	Peter Ruhdal Jensen	Künstliche promotorbibliotheken für ausgewählte organismen sowie daraus entstammende promotoren
SK7702000A3 (en) *	1998-09-25	2000-10-09	Ajinomoto Kk	Process for preparing coryneform bacteria having an improved amino acid- or nucleic acid-productivity
RU2194076C2 (ru) *	1998-10-19	2002-12-10	Адзиномото Ко., Инк.	Способ получения l-глутаминовой кислоты
US6255086B1 (en) *	1999-02-01	2001-07-03	Ajinomoto Co., Inc.	Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine
US7160705B2 (en) *	2000-04-28	2007-01-09	Ajinomoto Co., Inc.	Arginine repressor deficient strain of coryneform bacterium and method for producing L-arginine

1999
- 1999-09-22 SK SK770-2000A patent/SK7702000A3/sk unknown
- 1999-09-22 AT AT99944770T patent/ATE447039T1/de not_active IP Right Cessation
- 1999-09-22 HU HU0100045A patent/HU224975B1/hu unknown
- 1999-09-22 EP EP99944770A patent/EP1033407B1/de not_active Expired - Lifetime
- 1999-09-22 WO PCT/JP1999/005175 patent/WO2000018935A1/ja not_active Ceased
- 1999-09-22 ID IDW20000964A patent/ID25513A/id unknown
- 1999-09-22 HU HU0100045D patent/HU225541B1/hu unknown
- 1999-09-22 BR BRPI9917880-0A patent/BR9917880B1/pt active IP Right Grant
- 1999-09-22 CN CNB998023809A patent/CN1170938C/zh not_active Expired - Lifetime
- 1999-09-22 DE DE69941594T patent/DE69941594D1/de not_active Expired - Lifetime
- 1999-09-22 RU RU2000112926/13A patent/RU2214456C2/ru not_active IP Right Cessation
- 1999-09-22 ES ES99944770T patent/ES2335828T3/es not_active Expired - Lifetime
- 1999-09-22 BR BRPI9909409A patent/BRPI9909409B1/pt not_active IP Right Cessation
- 1999-09-22 KR KR1020007005666A patent/KR100630604B1/ko not_active Expired - Fee Related
- 1999-09-24 MY MYPI99004141A patent/MY130756A/en unknown
- 1999-09-24 PE PE1999000974A patent/PE20001111A1/es not_active Application Discontinuation
- 1999-09-27 TW TW088116544A patent/TWI247805B/zh not_active IP Right Cessation
- 1999-09-27 TW TW093129853A patent/TWI247807B/zh not_active IP Right Cessation
- 1999-09-27 TW TW093129852A patent/TWI247806B/zh not_active IP Right Cessation
- 1999-09-27 TW TW093129855A patent/TWI247808B/zh not_active IP Right Cessation
2000
- 2000-05-25 US US09/577,005 patent/US6962805B2/en not_active Expired - Lifetime
2005
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Also Published As

Publication number	Publication date
BR9909409A (pt)	2000-11-21
US6962805B2 (en)	2005-11-08
PE20001111A1 (es)	2000-10-31
US7608437B2 (en)	2009-10-27
EP1033407A1 (de)	2000-09-06
CN1170938C (zh)	2004-10-13
ID25513A (id)	2000-10-05
HU224975B1 (en)	2006-04-28
ATE447039T1 (de)	2009-11-15
TW200504213A (en)	2005-02-01
KR20010032426A (ko)	2001-04-16
BR9917880B1 (pt)	2014-02-18
EP1033407B1 (de)	2009-10-28
HU225541B1 (en)	2007-03-28
DE69941594D1 (de)	2009-12-10
BRPI9909409B1 (pt)	2016-03-22
US20060003424A1 (en)	2006-01-05
TW200504215A (en)	2005-02-01
US20040002143A1 (en)	2004-01-01
EP1033407A4 (de)	2004-08-18
HUP0100045A3 (en)	2003-01-28
ES2335828T3 (es)	2010-04-05
WO2000018935A1 (en)	2000-04-06
TW200504214A (en)	2005-02-01
HUP0100045A2 (hu)	2001-05-28
MY130756A (en)	2007-07-31
TWI247805B (en)	2006-01-21
TWI247806B (en)	2006-01-21
CN1289368A (zh)	2001-03-28
TWI247807B (en)	2006-01-21
KR100630604B1 (ko)	2006-10-04
TWI247808B (en)	2006-01-21
RU2214456C2 (ru)	2003-10-20

Publication	Publication Date	Title
SK7702000A3 (en)	2000-10-09	Process for preparing coryneform bacteria having an improved amino acid- or nucleic acid-productivity
RU2197528C2 (ru)	2003-01-27	Способ получения l-лизина (варианты) и рекомбинантная днк, используемая для его осуществления (варианты)
JP4595506B2 (ja)	2010-12-08	Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造方法
JP4035855B2 (ja)	2008-01-23	Ｌ−リジンの製造法
EP0857784B1 (de)	2006-09-20	Verfahren zur Herstellung von L-Lysin
JPWO1996040934A1 (ja)	1999-06-22	Ｌ−リジンの製造法
US20070172932A1 (en)	2007-07-26	L-Glutamic Acid-Producing Microorganism and a Method for Producing L-Glutamic Acid
JP3937726B2 (ja)	2007-06-27	発酵法によるｌ−グルタミン酸の製造法
JPWO1999007853A1 (ja)	2002-08-13	発酵法によるｌ−グルタミン酸の製造法
JP2000197490A (ja)	2000-07-18	Ｌ―アルギニンの製造法
JP3651002B2 (ja)	2005-05-25	アミノ酸生産菌の構築方法及び構築されたアミノ酸生産菌を用いる醗酵法によるアミノ酸の製造法
AU737421B2 (en)	2001-08-16	Process for constructing amino acid-producing bacterium and process for producing amino acid by fermentation method with the use of the thus constructed amino acid-producing bacterium
AU757594B2 (en)	2003-02-27	Process for constructing amino acid-producing bacterium and process for producing amino acid by fermentation method with the use of the thus constructed amino acid-producing bacterium
JPH1087A (ja)	1998-01-06	発酵法による目的物質の製造法
JP2000232890A (ja)	2000-08-29	発酵法によるｌ−グルタミン酸の製造法
MXPA00005186A (en)	2001-07-03	Process for constructing amino acid-producing bacterium and process for producing amino acid by fermentation method with the use of the thus constructed amino acid-producing bacterium
JPWO2000018935A1 (ja)	2001-12-18	アミノ酸生産菌の構築方法及び構築されたアミノ酸生産菌を用いる醗酵法によるアミノ酸の製造法