UA102854C2 - Способ и устройство для сортирования клеток - Google Patents

Способ и устройство для сортирования клеток

Info

Publication number
UA102854C2
UA102854C2 UAA201100874A UAA201100874A UA102854C2 UA 102854 C2 UA102854 C2 UA 102854C2 UA A201100874 A UAA201100874 A UA A201100874A UA A201100874 A UAA201100874 A UA A201100874A UA 102854 C2 UA102854 C2 UA 102854C2
Authority
UA
Ukraine
Prior art keywords
cells
determination
sorting
objective lens
analyte
Prior art date
Application number
UAA201100874A
Other languages
English (en)
Ukrainian (uk)
Inventor
Марк ЛАШЕР
Original Assignee
Микробикс Байосистемз Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Микробикс Байосистемз Инк. filed Critical Микробикс Байосистемз Инк.
Publication of UA102854C2 publication Critical patent/UA102854C2/ru

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N15/1409—Handling samples, e.g. injecting samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Sustainable Development (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Изобретение относится к устройству – сортировальному проточному цитомеру, который включает линзу объектива, имеющую оптическую ось, размещенную коаксиально с путем потока в фокусной точке. Контролированный источник энергии выборочно видоизменяет аналит (клетки пробы), для определения, принадлежит ли данный аналит к желаемой субпопуляции, фокусирование вторичного излучения из клетки линзой объектива, обнаружение сфокусированного вторичного излучения из отдельных клеток, определения клеток по интересу, отбор клеток. Изобретение также относится к способу и системе для дифференциального сортирования клеток.
UAA201100874A 2008-06-30 2009-06-30 Способ и устройство для сортирования клеток UA102854C2 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7708308P 2008-06-30 2008-06-30
PCT/IB2009/006480 WO2010001254A2 (en) 2008-06-30 2009-06-30 Method and apparatus for sorting cells

Publications (1)

Publication Number Publication Date
UA102854C2 true UA102854C2 (ru) 2013-08-27

Family

ID=41447919

Family Applications (2)

Application Number Title Priority Date Filing Date
UAA201100874A UA102854C2 (ru) 2008-06-30 2009-06-30 Способ и устройство для сортирования клеток
UAA201305229A UA115521C2 (uk) 2008-06-30 2009-06-30 Метод і пристосування для сортування клітин

Family Applications After (1)

Application Number Title Priority Date Filing Date
UAA201305229A UA115521C2 (uk) 2008-06-30 2009-06-30 Метод і пристосування для сортування клітин

Country Status (13)

Country Link
US (2) US8004661B2 (ru)
EP (1) EP2304020B1 (ru)
CN (1) CN102119212B (ru)
BR (1) BRPI0914919A2 (ru)
CA (1) CA2728289C (ru)
DK (1) DK2304020T3 (ru)
ES (1) ES2709073T3 (ru)
MX (1) MX2011000182A (ru)
NZ (1) NZ590166A (ru)
PL (1) PL2304020T3 (ru)
RU (1) RU2520848C2 (ru)
UA (2) UA102854C2 (ru)
WO (1) WO2010001254A2 (ru)

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CN102119212A (zh) 2011-07-06
ES2709073T3 (es) 2019-04-15
EP2304020A4 (en) 2017-10-11
WO2010001254A2 (en) 2010-01-07
WO2010001254A3 (en) 2010-04-01
DK2304020T3 (en) 2019-02-04
EP2304020B1 (en) 2018-11-07
EP2304020A2 (en) 2011-04-06
CA2728289C (en) 2016-11-29
US8467040B2 (en) 2013-06-18
NZ590166A (en) 2013-09-27
MX2011000182A (es) 2011-08-03
BRPI0914919A2 (pt) 2015-08-25
US20090325217A1 (en) 2009-12-31
RU2011102290A (ru) 2012-08-10
PL2304020T3 (pl) 2019-06-28
CA2728289A1 (en) 2010-01-07
US20120038914A1 (en) 2012-02-16
US8004661B2 (en) 2011-08-23
RU2520848C2 (ru) 2014-06-27
UA115521C2 (uk) 2017-11-27

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