UA35599C2 - Способ очистки гемоглобина из сырого раствора - Google Patents
Способ очистки гемоглобина из сырого раствораInfo
- Publication number
- UA35599C2 UA35599C2 UA95058439A UA95058439A UA35599C2 UA 35599 C2 UA35599 C2 UA 35599C2 UA 95058439 A UA95058439 A UA 95058439A UA 95058439 A UA95058439 A UA 95058439A UA 35599 C2 UA35599 C2 UA 35599C2
- Authority
- UA
- Ukraine
- Prior art keywords
- hemoglobin
- column
- admixtures
- raw solution
- stage
- Prior art date
Links
- 102000001554 Hemoglobins Human genes 0.000 title abstract 9
- 108010054147 Hemoglobins Proteins 0.000 title abstract 9
- 238000000034 method Methods 0.000 title abstract 2
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 238000011097 chromatography purification Methods 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/422—Displacement mode
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Это изобретение касается очистки гемоглобина хроматографией. Способ очистки гемоглобина из сырого раствора, содержащего загрязняющие протеиноподобные вещества, путем ионообменной хроматографии. Сырой раствор используют в концентрации 0,1-20%, раствор на первой стадии пропускают через колонку, заполненную либо анионообменником для очистки сырого раствора, в котором загрязняющие вещества имеют более кислую реакцию, чем гемоглобин, либо катионообменником для очистки сырого раствора, в котором загрязняющие вещества имеют более основную реакцию, чем гемоглобин, при этом непрерывное пропускание сырого раствора на первой стадии осуществляют вначале до полного насыщения колонки гемоглобином и компонентами с высоким сродством к соответствующему ионообменнику, а в дальнейшем - до сверхнагрузки колонки с последующим замещением на ней гемоглобина, на второй стадии осуществляют пропускание элюата, полученного на первой стадии, через анионо- или катионообменник, не выбранный на первой стадии, при этом хроматографию осуществляют также вначале до насыщения колонки гемоглобином и веществами с более высоким сродством к колонке, а затем до сверхнагрузки и замещения на ней гемоглобина.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002106612A CA2106612C (en) | 1993-09-21 | 1993-09-21 | Displacement chromatography process |
| PCT/CA1994/000514 WO1995008574A1 (en) | 1993-09-21 | 1994-09-20 | Displacement chromatography process and purified hemoglobin product |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA35599C2 true UA35599C2 (ru) | 2001-04-16 |
Family
ID=4152343
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UA95058439A UA35599C2 (ru) | 1993-09-21 | 1994-09-20 | Способ очистки гемоглобина из сырого раствора |
Country Status (9)
| Country | Link |
|---|---|
| JP (1) | JP4057049B2 (ru) |
| KR (1) | KR100361894B1 (ru) |
| AT (1) | ATE175975T1 (ru) |
| DE (1) | DE69416103T2 (ru) |
| DK (1) | DK0668877T3 (ru) |
| ES (1) | ES2126775T3 (ru) |
| HU (1) | HU218159B (ru) |
| RU (1) | RU2145873C1 (ru) |
| UA (1) | UA35599C2 (ru) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006116064A2 (en) * | 2005-04-22 | 2006-11-02 | Waters Investments Limited | Apparatus and methods for mass-spectrometric directed purification of biopolymers |
| RU2329826C1 (ru) * | 2006-12-29 | 2008-07-27 | Институт высокомолекулярных соединений РАН (ИВС РАН) | Способ получения растворов гемоглобина, очищенных от стромальных компонентов |
| PT2462158T (pt) | 2009-08-06 | 2018-02-23 | H Hoffnabb La Roche Ag | Método para melhorar a remoção de vírus na purificação de proteínas |
| WO2012068134A1 (en) * | 2010-11-15 | 2012-05-24 | Biogen Idec Inc. | Enrichment and concentration of select product isoforms by overloaded bind and elute chromatography |
| ES2697676T3 (es) | 2011-12-22 | 2019-01-25 | Hoffmann La Roche | Cromatografía de membrana de intercambio iónico |
| DK2994485T3 (en) * | 2013-05-06 | 2018-04-23 | Sanofi Sa | CONTINUOUS MULTIPLE STEP PROCEDURE FOR PURIFICATION OF ANTIBODIES |
| EP3546475A1 (en) | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| TW202128264A (zh) * | 2019-09-25 | 2021-08-01 | 瑞士商赫孚孟拉羅股份公司 | 使用經預測之溶離緩衝鹽濃度的陽離子層析法 |
| KR20240128671A (ko) * | 2021-10-27 | 2024-08-26 | 플라즈마 테크놀로지스, 엘엘씨 | 단백질을 단리하기 위한 조성물 및 방법(compositions and methods for isolating proteins) |
| CN114712894B (zh) * | 2022-04-08 | 2024-04-30 | 无锡博慧斯生物医药科技有限公司 | 一种糖化血红蛋白层析柱的活化液、活化方法和应用 |
-
1994
- 1994-09-20 HU HU9501479A patent/HU218159B/hu not_active IP Right Cessation
- 1994-09-20 DE DE69416103T patent/DE69416103T2/de not_active Expired - Fee Related
- 1994-09-20 JP JP50945895A patent/JP4057049B2/ja not_active Expired - Fee Related
- 1994-09-20 DK DK94926756T patent/DK0668877T3/da active
- 1994-09-20 RU RU95112503A patent/RU2145873C1/ru not_active IP Right Cessation
- 1994-09-20 AT AT94926756T patent/ATE175975T1/de not_active IP Right Cessation
- 1994-09-20 UA UA95058439A patent/UA35599C2/ru unknown
- 1994-09-20 ES ES94926756T patent/ES2126775T3/es not_active Expired - Lifetime
- 1994-09-21 KR KR1019950701487A patent/KR100361894B1/ko not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| KR100361894B1 (ko) | 2003-05-12 |
| DE69416103T2 (de) | 1999-08-05 |
| DK0668877T3 (da) | 1999-09-13 |
| DE69416103D1 (de) | 1999-03-04 |
| HU218159B (hu) | 2000-06-28 |
| RU95112503A (ru) | 1996-12-20 |
| HU9501479D0 (en) | 1995-07-28 |
| ATE175975T1 (de) | 1999-02-15 |
| RU2145873C1 (ru) | 2000-02-27 |
| JP4057049B2 (ja) | 2008-03-05 |
| JPH08503714A (ja) | 1996-04-23 |
| ES2126775T3 (es) | 1999-04-01 |
| HUT72924A (en) | 1996-06-28 |
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