UA99263C2 - СПОСОБ ОЧИСТКИ Fc-СЛИТОГО БЕЛКА - Google Patents

СПОСОБ ОЧИСТКИ Fc-СЛИТОГО БЕЛКА

Info

Publication number
UA99263C2
UA99263C2 UAA200902919A UAA200902919A UA99263C2 UA 99263 C2 UA99263 C2 UA 99263C2 UA A200902919 A UAA200902919 A UA A200902919A UA A200902919 A UAA200902919 A UA A200902919A UA 99263 C2 UA99263 C2 UA 99263C2
Authority
UA
Ukraine
Prior art keywords
fusion protein
purification
chromatography
protein
exchange chromatography
Prior art date
Application number
UAA200902919A
Other languages
English (en)
Ukrainian (uk)
Inventor
Алекс Эон-Дюваль
Ален Лампруа
Original Assignee
Арес Трейдинг С.А.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Арес Трейдинг С.А. filed Critical Арес Трейдинг С.А.
Publication of UA99263C2 publication Critical patent/UA99263C2/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C07K1/28—Isoelectric focusing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Изобретение относится к способу очистки Fc-слитого белка, который имеет изоэлектрическую точку (pI) 6,9-9,5, который предусматривает подвергание жидкости, которая содержит указанный Fc-слитый белок, белок А- или белок G-аффинной хроматографии с последующей катионообменной хроматографией, анионообменной хроматографией и гидроксиапатитной хроматографией и сбор элюата для получения очищенного Fc-слитого белка.
UAA200902919A 2006-08-28 2007-08-27 СПОСОБ ОЧИСТКИ Fc-СЛИТОГО БЕЛКА UA99263C2 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP06119610 2006-08-28

Publications (1)

Publication Number Publication Date
UA99263C2 true UA99263C2 (ru) 2012-08-10

Family

ID=37038258

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA200902919A UA99263C2 (ru) 2006-08-28 2007-08-27 СПОСОБ ОЧИСТКИ Fc-СЛИТОГО БЕЛКА

Country Status (15)

Country Link
US (1) US20100267932A1 (ru)
EP (1) EP2061802A1 (ru)
KR (1) KR20090058532A (ru)
CN (1) CN101541825B (ru)
AR (1) AR062569A1 (ru)
AU (1) AU2007291282B2 (ru)
BR (1) BRPI0716139A2 (ru)
CA (1) CA2661872A1 (ru)
EA (1) EA017733B1 (ru)
IL (1) IL197221A (ru)
MX (1) MX2009002013A (ru)
NZ (1) NZ574623A (ru)
UA (1) UA99263C2 (ru)
WO (1) WO2008025747A1 (ru)
ZA (1) ZA200900836B (ru)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR058568A1 (es) 2005-12-20 2008-02-13 Bristol Myers Squibb Co Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo
PT2253644E (pt) 2005-12-20 2014-01-23 Bristol Myers Squibb Co Composições e métodos para a produção de uma composição
DK2089429T3 (en) * 2006-10-19 2015-01-12 Janssen Biotech Inc PROCEDURE FOR MANUFACTURING NON-AGGREGATED ANTIBODY-FC DOMAINS
WO2008085988A1 (en) * 2007-01-05 2008-07-17 Amgen Inc. Methods of purifying proteins
WO2011015926A1 (en) * 2009-08-03 2011-02-10 Avesthagen Limited A process of fermentation, purification and production of recombinant soluble tumour necrosis factor alfa receptor (tnfr) - human igg fc fusion protein
EP4786482A2 (en) 2009-08-06 2026-08-05 F. Hoffmann-La Roche AG Virus filtration methods
US8536316B2 (en) * 2009-08-07 2013-09-17 Emd Millipore Corporation Methods for purifying a target protein from one or more impurities in a sample
BR112012004697B8 (pt) * 2009-09-01 2021-05-25 Genentech Inc método para purificar um polipeptídeo compreendendo uma região ch2/ch3
US20120178910A1 (en) 2009-09-23 2012-07-12 Medarex, Inc. Cation exchange chromatography (methods)
WO2011090719A2 (en) * 2009-12-29 2011-07-28 Dr. Reddy's Laboratories Ltd. Protein purification by ion exchange
JP2013519652A (ja) * 2010-02-12 2013-05-30 ディーエスエム アイピー アセッツ ビー．ブイ． 単一ユニット抗体精製
WO2012030512A1 (en) * 2010-09-03 2012-03-08 Percivia Llc. Flow-through protein purification process
EA201300524A1 (ru) * 2010-11-01 2013-08-30 ДСМ АйПи АССЕТС Б.В. Очистка антител ионообменной хроматографией в едином блоке
EP2691411B1 (en) 2011-03-29 2020-02-26 GlaxoSmithKline LLC Buffer system for protein purification
EP2714737A1 (en) * 2011-05-27 2014-04-09 Glaxo Group Limited Bcma (cd269/tnfrsf17) -binding proteins
UA112434C2 (uk) 2011-05-27 2016-09-12 Ґлаксо Ґруп Лімітед Антигензв'язувальний білок, який специфічно зв'язується з всма
EP2723759A4 (en) * 2011-06-24 2015-01-28 Reddys Lab Ltd Dr PURIFICATION OF CHIMERIC PROTEIN
SMT201900520T1 (it) * 2011-06-28 2019-11-13 Inhibrx Lp Polipeptidi di fusione serpinici e relativi metodi d'uso
JP6280499B2 (ja) * 2011-07-08 2018-02-14 メルク・シャープ・アンド・ドーム・コーポレーションＭｅｒｃｋ Ｓｈａｒｐ ＆ Ｄｏｈｍｅ Ｃｏｒｐ． Ｆｃ融合タンパク質の精製方法
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
KR20140091721A (ko) * 2011-11-02 2014-07-22 제넨테크, 인크. 오버로드 및 용리 크로마토그래피
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
CN104861060A (zh) * 2014-02-21 2015-08-26 神州细胞工程有限公司 一种纯化凝血因子viii的方法
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
WO2017168296A1 (en) * 2016-03-29 2017-10-05 Navya Biologicals Pvt. Ltd A process for purification of fc-fusion proteins
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
JP7122974B2 (ja) 2016-05-11 2022-08-22 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 変異免疫グロブリン結合ポリペプチド
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
WO2017194592A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Method of storing a separation matrix
EP3455241B1 (en) 2016-05-11 2022-02-23 Cytiva BioProcess R&D AB Method of cleaning and/or sanitizing a separation matrix
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
JP2019530646A (ja) * 2016-08-12 2019-10-24 ブリストル−マイヤーズ スクイブ カンパニーＢｒｉｓｔｏｌ−Ｍｙｅｒｓ Ｓｑｕｉｂｂ Ｃｏｍｐａｎｙ タンパク質精製法
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
US12516102B1 (en) * 2017-02-09 2026-01-06 Bracco Suisse Sa Process for the purification of soluble PSGL-1 protein variants
CN107151673A (zh) * 2017-03-10 2017-09-12 上海埃文生物科技有限公司 巴斯德毕赤酵母中人rank胞外段蛋白的表达纯化及其应用
WO2019043096A1 (en) * 2017-08-30 2019-03-07 Fresenius Kabi Deutschland Gmbh METHOD FOR PURIFYING IL-6 ANTI-RECEPTOR ANTIBODIES
EP3676281A1 (en) * 2017-08-30 2020-07-08 Ares Trading S.A. Method for purifying proteins
EP3694870A1 (en) 2017-10-10 2020-08-19 Alpine Immune Sciences, Inc. Ctla-4 variant immunomodulatory proteins and uses thereof
CA3085177A1 (en) * 2018-01-31 2019-08-08 Regeneron Pharamaceuticals, Inc. System and method for characterizing size and charge variant drug product impurities
US20240228573A9 (en) * 2019-10-17 2024-07-11 Jcr Pharmaceuticals Co., Ltd. Method for producing fusion protein of serum albumin and growth hormone
IL309560B2 (en) * 2019-12-06 2025-05-01 Regeneron Pharma Anti-VEGF protein preparations and methods for their production
IL296039A (en) * 2020-03-06 2022-10-01 Aleta Biotherapeutics Inc Compositions and methods for treatment of cancer
MX2022013812A (es) 2020-05-08 2022-12-15 Regeneron Pharma Trampas y mini-trampas de vegf y metodos para el tratamiento de trastornos oculares y cancer.
US12202882B2 (en) 2020-05-08 2025-01-21 Alpine Immune Sciences, Inc. APRIL and BAFF inhibitory immunomodulatory proteins and methods of use thereof
CN116322775A (zh) 2020-06-02 2023-06-23 阿雷斯贸易股份有限公司 IgA肾病治疗的相关方法
TWI891671B (zh) * 2020-10-29 2025-08-01 日商Ｊｃｒ製藥股份有限公司 血清白蛋白與生長激素之融合蛋白質之製造方法
CN112574321B (zh) * 2020-12-30 2023-10-20 上海赛金生物医药有限公司 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法
CN114292328B (zh) * 2021-12-30 2025-07-25 武汉原谷生物科技有限责任公司 一种来源于美洲羊驼中天然抗体的纯化方法及其应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5447851B1 (en) * 1992-04-02 1999-07-06 Univ Texas System Board Of Dna encoding a chimeric polypeptide comprising the extracellular domain of tnf receptor fused to igg vectors and host cells
NZ256293A (en) * 1992-09-15 1997-06-24 Immunex Corp Method of treating tumour necrosis factor - mediated inflammation by use of tnf antagonist
ATE175979T1 (de) * 1993-12-27 1999-02-15 Rotkreuzstiftung Zentrallab Verfahren zur herstellung eines konzentrates von anti-d-immunoglobulin g und pharmazeutische zusammensetzung, die dieses enthält
US6406901B1 (en) * 1995-06-08 2002-06-18 Immunex Corporation TNF-a converting enzyme
ES2227598T3 (es) * 1995-06-08 2005-04-01 Immunex Corporation Enzima conversiva de tnf-alfa.
US5969102A (en) * 1997-03-03 1999-10-19 St. Jude Children's Research Hospital Lymphocyte surface receptor that binds CAML, nucleic acids encoding the same and methods of use thereof
ATE370961T1 (de) * 1998-05-06 2007-09-15 Genentech Inc Reinigung von antikörpern durch ionenaustauschchromatographie
AU2001263114A1 (en) * 2000-05-12 2001-11-26 Amgen Inc Methods and compositions of matter concerning april/g70, bcma, blys/agp-3, and taci
PL403488A1 (pl) * 2001-05-24 2013-07-08 Zymogenetics, Inc. Zastosowanie bialka fuzyjnego TACI-immunoglobulina, bialko fuzyjne, czasteczka kwasu nukleinowego kodujaca bialko fuzyjne, kompozycja farmaceutyczna i sposób wytwarzania bialka fuzyjnego TACI-immunoglobulina
JP4363986B2 (ja) * 2001-12-21 2009-11-11 イミュネックス・コーポレーション タンパク質の精製方法
SI1501369T1 (sl) * 2002-04-26 2015-10-30 Genentech, Inc. Neafinitetno čiščenje proteinov
CN100522989C (zh) * 2002-09-11 2009-08-05 中外制药株式会社 纯化蛋白质的方法
US20060257972A1 (en) * 2003-03-31 2006-11-16 Takashi Ishihara Purification of human monoclonal antibody and human polyclonal antibody
TWI353991B (en) * 2003-05-06 2011-12-11 Syntonix Pharmaceuticals Inc Immunoglobulin chimeric monomer-dimer hybrids
BRPI0415887B1 (pt) * 2003-10-27 2021-01-26 Wyeth métodos para a remoção de agregados de elevado peso molecular utilizando cromatografia de hidroxiapatita
CN1234725C (zh) * 2004-04-07 2006-01-04 陈志南 一种高效快速纯化制备片段抗体的方法
BRPI0611600B8 (pt) * 2005-06-17 2021-05-25 Wyeth Corp método para purificação de uma proteína tendo uma região fc a partir de um líquido fonte compreendendo a proteína e uma ou mais impurezas, e kit
JP2010516651A (ja) * 2007-01-17 2010-05-20 メルク セローノ ソシエテ アノニム Ｆｃ含有タンパク質の精製のための方法

Also Published As

Publication number Publication date
KR20090058532A (ko) 2009-06-09
US20100267932A1 (en) 2010-10-21
AU2007291282B2 (en) 2011-10-20
CA2661872A1 (en) 2008-03-06
EA200970230A1 (ru) 2009-08-28
AR062569A1 (es) 2008-11-19
CN101541825A (zh) 2009-09-23
NZ574623A (en) 2011-12-22
IL197221A (en) 2013-06-27
EP2061802A1 (en) 2009-05-27
BRPI0716139A2 (pt) 2013-09-17
ZA200900836B (en) 2010-05-26
WO2008025747A1 (en) 2008-03-06
IL197221A0 (en) 2009-12-24
MX2009002013A (es) 2009-03-09
EA017733B1 (ru) 2013-02-28
CN101541825B (zh) 2013-08-14
AU2007291282A1 (en) 2008-03-06

Similar Documents

Publication Publication Date Title
EA200970230A1 (ru) СПОСОБ ОЧИСТКИ Fc-СЛИТЫХ БЕЛКОВ
SG10201407983VA (en) Method of purifying polypeptides
IL175431A0 (en) Purification of il-18 binding protein
IL187935A0 (en) Process for the purification of il-18 binding protein
TW200505837A (en) Novel compounds
WO2007081851A3 (en) Affinity chromatography matrices and methods of making and using the same
WO2003102132A3 (en) Non-affinity purification of proteins
TW200612892A (en) Novel compounds
PE20150720A1 (es) Purificacion de iduronato-2-sulfatasa
TW200510303A (en) Novel compounds
UA99262C2 (ru) СПОСОБ УМЕНЬШЕНИЯ КОНЦЕНТРАЦИИ СВОБОДНЫХ FC-частиц В ЖИДКОСТИ, КОТОРАЯ СОДЕРЖИТ Fc-содержащий БЕЛОК
IL166438A (en) Method for purifying preproinsulin
TW200508201A (en) Novel process for the preparation of roflumilast
MX348178B (es) Método para purificar adamts13 recombinante y otras proteínas y sus composiciones.
TNSN03056A1 (fr) Procede d'obtention d'hydroxytyrosol purifie a partir des produits et sous-produits derives de l'olivier
PL1664108T3 (pl) Rozdzielanie polipeptydów zawierających racemizowany aminokwas
GB0100513D0 (en) Process
WO2011090720A3 (en) Purification of proteins
GB0216001D0 (en) Process and composition
BRPI0417992A (pt) método para purificar fsh
WO2007016635A3 (en) Process for the purification of coenzyme q10
WO2004029072A3 (en) PrPsc -INTERACTING MOLECULES AND USES THEREOF
PL1841863T3 (pl) Sposób oczyszczania czynnika VII
MY157846A (en) Process for the purification of fc-containing proteins
DE602005013263D1 (de) Reinigung von glykopeptiden