WO2012129373A3 - Procédés de production d'un locus complexe de caractéristique transgénique - Google Patents

Procédés de production d'un locus complexe de caractéristique transgénique Download PDF

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Publication number
WO2012129373A3
WO2012129373A3 PCT/US2012/030061 US2012030061W WO2012129373A3 WO 2012129373 A3 WO2012129373 A3 WO 2012129373A3 US 2012030061 W US2012030061 W US 2012030061W WO 2012129373 A3 WO2012129373 A3 WO 2012129373A3
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WO
WIPO (PCT)
Prior art keywords
transgenic trait
complex transgenic
methods
producing
trait locus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/030061
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English (en)
Other versions
WO2012129373A2 (fr
Inventor
Michael Lassner
Derek Jantz
James Jefferson Smith
Mark CIGAN
Carl Falco
Huirong Gao
Zhongsen Li
Zhan-Bin Liu
Sergei Svitashev
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
EIDP Inc
Original Assignee
Pioneer Hi Bred International Inc
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred International Inc, EI Du Pont de Nemours and Co filed Critical Pioneer Hi Bred International Inc
Priority to EP12712040.0A priority Critical patent/EP2689019A2/fr
Priority to CN201280022453.3A priority patent/CN103842511A/zh
Priority to BR112013024337A priority patent/BR112013024337A2/pt
Priority to EA201391373A priority patent/EA201391373A1/ru
Priority to MX2013010911A priority patent/MX2013010911A/es
Priority to CA2831144A priority patent/CA2831144A1/fr
Publication of WO2012129373A2 publication Critical patent/WO2012129373A2/fr
Publication of WO2012129373A3 publication Critical patent/WO2012129373A3/fr
Anticipated expiration legal-status Critical
Priority to ZA2013/07200A priority patent/ZA201307200B/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des procédés de production, dans une plante, d'un locus complexe de caractéristique transgénique, comprenant au moins deux séquences cibles modifiées dans une région génomique d'intérêt. Les procédés consistent à utiliser au moins deux agents induisant une coupure double brin, dont chacun peut provoquer une coupure double brin dans une séquence cible dans la région génomique d'intérêt, ce qui conduit à une modification dans la séquence cible. L'invention concerne également des loci complexes de caractéristiques transgéniques dans les plantes. Un locus complexe de caractéristique transgénique comprend au moins deux séquences cibles modifiées qui sont liées génétiquement à un polynucléotide d'intérêt. L'invention concerne aussi des plantes, des cellules végétales, des parties de plantes et des semences comprenant un ou plusieurs loci complexes de caractéristique transgénique.
PCT/US2012/030061 2011-03-23 2012-03-22 Procédés de production d'un locus complexe de caractéristique transgénique Ceased WO2012129373A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP12712040.0A EP2689019A2 (fr) 2011-03-23 2012-03-22 Procédés de production d'un locus complexe de caractéristique transgénique
CN201280022453.3A CN103842511A (zh) 2011-03-23 2012-03-22 产生复合转基因性状基因座的方法
BR112013024337A BR112013024337A2 (pt) 2011-03-23 2012-03-22 locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão
EA201391373A EA201391373A1 (ru) 2011-03-23 2012-03-22 Способы получения сложного локуса трансгенных признаков
MX2013010911A MX2013010911A (es) 2011-03-23 2012-03-22 Metodos para producir un locus de rasgo transgenico complejo.
CA2831144A CA2831144A1 (fr) 2011-03-23 2012-03-22 Procedes de production d'un locus complexe de caracteristique transgenique
ZA2013/07200A ZA201307200B (en) 2011-03-23 2013-09-26 Methods for producing a complex transgenic trait locus

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161466602P 2011-03-23 2011-03-23
US61/466,602 2011-03-23
US201161499443P 2011-06-21 2011-06-21
US61/499,443 2011-06-21

Publications (2)

Publication Number Publication Date
WO2012129373A2 WO2012129373A2 (fr) 2012-09-27
WO2012129373A3 true WO2012129373A3 (fr) 2013-03-14

Family

ID=45929041

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/030061 Ceased WO2012129373A2 (fr) 2011-03-23 2012-03-22 Procédés de production d'un locus complexe de caractéristique transgénique

Country Status (10)

Country Link
US (5) US10030245B2 (fr)
EP (1) EP2689019A2 (fr)
CN (1) CN103842511A (fr)
AR (1) AR085533A1 (fr)
BR (1) BR112013024337A2 (fr)
CA (1) CA2831144A1 (fr)
EA (1) EA201391373A1 (fr)
MX (1) MX2013010911A (fr)
WO (1) WO2012129373A2 (fr)
ZA (1) ZA201307200B (fr)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2689019A2 (fr) 2011-03-23 2014-01-29 Pioneer Hi-Bred International Inc. Procédés de production d'un locus complexe de caractéristique transgénique
EP2790708B1 (fr) 2011-12-16 2024-10-23 Targetgene Biotechnologies Ltd. Compositions et procédés pour la modification d'une séquence d'acide nucléique cible prédéterminée
AR089793A1 (es) * 2012-01-27 2014-09-17 Du Pont Metodos y composiciones para generar locus de rasgos transgenicos complejos
UA123532C2 (uk) 2013-03-12 2021-04-21 Е. І. Дю Пон Де Немур Енд Компані Спосіб ідентифікації варіантного сайта розпізнавання для сконструйованого засобу, що рідко розщеплює, для індукції двониткового розриву
CA3221516A1 (fr) 2013-08-22 2015-02-26 E. I. Du Pont De Nemours And Company Modification du genome des plantes a l'aide de systemes d'arn de guida /endonuclease cas et leurs procedes d'utilisation
CN106687594A (zh) 2014-07-11 2017-05-17 纳幕尔杜邦公司 用于产生对草甘膦除草剂具有抗性的植物的组合物和方法
BR112017003757A2 (pt) 2014-09-12 2017-12-26 Du Pont ?plantas de milho, partes de planta de milho ou sementes de milho?
EP4696781A3 (fr) 2014-09-26 2026-04-01 Pioneer Hi-Bred International, Inc. Polynucleotides, polypeptides de la ms1 du ble et procedes d'utilisation
WO2016100309A1 (fr) 2014-12-16 2016-06-23 Pioneer Hi-Bred International, Inc. Rétablissement de la fertilité mâle dans le blé
EP3237624B1 (fr) 2014-12-23 2020-01-29 Syngenta Participations AG Procédés et compositions d'identification et d'enrichissement pour des cellules contenant des modifications génomiques spécifiques à un site
ES2945315T3 (es) 2015-01-27 2023-06-30 Inst Genetics & Developmental Biology Cas Método para realizar modificaciones específicas de sitio en la planta completa a través de la expresión transitoria de genes
EP3262176A1 (fr) 2015-02-25 2018-01-03 Pioneer Hi-Bred International, Inc. Composition et procédés pour la régulation de l'expression d'un complexe arn guide/endonucléase cas
KR102194612B1 (ko) 2015-03-16 2020-12-23 인스티튜트 오브 제네틱스 앤드 디벨롭멘털 바이오롤지, 차이니즈 아카데미 오브 사이언시스 비-유전성 물질을 이용한 식물 게놈의 부위-특이적인 변형 방법
EP3553178A1 (fr) 2015-03-27 2019-10-16 E. I. du Pont de Nemours and Company Promoteurs de petit gène d'arn nucléaire u6 de graines de soja et leur utilisation dans l'expression de constitutive de petits gènes dans des plantes
EP3294878A1 (fr) 2015-05-15 2018-03-21 Pioneer Hi-Bred International, Inc. Systèmes arn guide/endonucléase cas
EP3095870A1 (fr) 2015-05-19 2016-11-23 Kws Saat Se Procédés pour la transformation in planta de plantes et procédés de fabrication basés sur ceux-ci et produits pouvant être obtenus à partir de ceux-ci
EP3316676A4 (fr) * 2015-06-30 2018-12-12 Regents of the University of Minnesota Lignée inductrice d'haploïdes pour l'édition de génome accélérée
JP2018527920A (ja) 2015-08-14 2018-09-27 インスティテュート・オブ・ジェネティクス・アンド・ディヴェロプメンタル・バイオロジー、チャイニーズ・アカデミー・オブ・サイエンシズＩｎｓｔｉｔｕｔｅ ｏｆ Ｇｅｎｅｔｉｃｓ ａｎｄ Ｄｅｖｅｌｏｐｍｅｎｔａｌ Ｂｉｏｌｏｇｙ， Ｃｈｉｎｅｓｅ Ａｃａｄｅｍｙ ｏｆ Ｓｃｉｅｎｃｅｓ 部位特異的ヌクレオチド置換によりグリホサート耐性イネを取得するための方法
FI4144844T3 (fi) 2015-10-12 2025-11-24 Dupont Us Holding Llc Suojatut dna-templaatit geenimuokkaukseen ja homologisen rekombinaation lisäämiseen soluissa ja niiden käyttömenetelmät
CN108138155A (zh) 2015-10-20 2018-06-08 先锋国际良种公司 经由指导cas系统恢复非功能性基因产物的功能及使用方法
US20180258438A1 (en) * 2015-11-06 2018-09-13 Pioneer Hi-Bred International, Inc. Generation of complex trait loci in soybean and methods of use
DK3387134T3 (da) 2015-12-11 2020-12-21 Danisco Us Inc Fremgangsmåder og sammensætninger til øget nukleasemedieret genommodifikation og reducerede virkninger uden for målstedet
DK3390631T3 (da) 2015-12-18 2020-07-13 Danisco Us Inc Fremgangsmåder og sammensætninger til t-rna-baseret guide-rna-ekspression
BR112018015348A2 (pt) 2016-01-26 2018-12-18 Pioneer Hi Bred Int método para obter uma planta de milho ceroso, planta, semente, polinucleotídeo-guia e método para produzir uma planta híbrida de milho ceroso
WO2017155715A1 (fr) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Nouveaux systèmes cas9 et procédés d'utilisation
EP3426780A1 (fr) 2016-03-11 2019-01-16 Pioneer Hi-Bred International, Inc. Nouveaux systèmes cas9 et procédés d'utilisation
CA3010628A1 (fr) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Nouveaux systemes cas9 et procedes d'utilisation
AU2017234920B2 (en) 2016-03-18 2023-08-17 E. I. Du Pont De Nemours And Company Methods and compositions for producing clonal, non-reduced, non-recombined gametes
MX2018014993A (es) 2016-06-14 2019-09-06 Pioneer Hi Bred Int Uso de endonucleasa cpf1 para modificaciones de genoma de planta.
WO2017222773A1 (fr) 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Nouveaux systèmes cas et méthodes d'utilisation
CA3037560A1 (fr) 2016-10-13 2018-04-19 Pioneer Hi-Bred International, Inc. Generation de mais resistant a l'helminthosporiose du nord
US20210032645A1 (en) * 2017-01-11 2021-02-04 Yeda Research And Development Co. Ltd. Targeted recombination between homologous chromosomes and uses thereof
EP3661354A1 (fr) 2017-07-31 2020-06-10 R. J. Reynolds Tobacco Company Procédés et compositions pour l'édition de gènes à base de virus dans des plantes
WO2019118463A1 (fr) 2017-12-15 2019-06-20 Danisco Us Inc Variants de cas9 et procédés d'utilisation
EP3755792A4 (fr) 2018-02-23 2021-12-08 Pioneer Hi-Bred International, Inc. Nouveaux orthologues de cas9
ES3036459T3 (en) * 2018-03-12 2025-09-19 Pioneer Hi Bred Int Methods for plant transformation
WO2019204256A1 (fr) 2018-04-18 2019-10-24 Pioneer Hi-Bred International, Inc. Amélioration de caractéristiques agronomiques dans le maïs par modification de facteurs de transcription de mads box endogènes
AU2019255192B2 (en) 2018-04-18 2023-02-02 Pioneer Hi-Bred International, Inc. Genes, constructs and maize event DP-202216-6
CN112088018A (zh) 2018-05-07 2020-12-15 先锋国际良种公司 用于在植物细胞基因组中同源定向修复双链断裂的方法和组合物
US20210324398A1 (en) 2018-06-29 2021-10-21 Pioneer Hi-Bred International, Inc. Edited nac genes in plants
CN113166744B (zh) 2018-12-14 2025-02-07 先锋国际良种公司 用于基因组编辑的新颖crispr-cas系统
US12031140B2 (en) * 2019-11-25 2024-07-09 Syngenta Crop Protection Ag Glycine regulatory elements and uses thereof
WO2021173480A2 (fr) 2020-02-24 2021-09-02 Pioneer Hi-Bred International, Inc. Recombinaison homologue intragénomique
US20220056470A1 (en) 2020-08-18 2022-02-24 Pioneer Hi-Bred International, Inc. Multiple disease resistance genes and genomic stacks thereof
US20250027097A1 (en) 2021-11-30 2025-01-23 Pioneer Hi-Bred International, Inc. High efficiency large scale chromosomal genome manipulation
JP2026510998A (ja) 2023-03-20 2026-04-10 パイオニア ハイ－ブレッド インターナショナル， インコーポレイテッド 変更されたＰＡＭ認識を有するＣａｓポリペプチド
WO2025184161A1 (fr) * 2024-02-27 2025-09-04 Dryland Genetics, Inc. Variétés de proso dlg197 et dlg317 à rendement supérieur

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008148559A1 (fr) * 2007-06-05 2008-12-11 Bayer Bioscience N.V. Procédés et moyens permettant le remplacement précis d'un adn cible dans des organismes eucaryotes
WO2009006297A2 (fr) * 2007-06-29 2009-01-08 Pioneer Hi-Bred International, Inc. Procédés de modification du génome d'une cellule de plante monocotylédone
WO2009042164A1 (fr) * 2007-09-27 2009-04-02 Dow Agrosciences Llc Protéines à doigt de zinc synthétisées ciblant des gènes de 5-énolpyruvyl shikimate-3-phosphate synthase
WO2009114321A2 (fr) * 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Méganucléases rationnellement conçues pour modification par génie génétique du génome du maïs
WO2010077319A1 (fr) * 2008-12-17 2010-07-08 Dow Agrosciences Llc Intégration ciblée dans le locus zp15
WO2011117249A1 (fr) * 2010-03-22 2011-09-29 Philip Morris Products S.A. Modification d'activité enzymatique dans des plantes

Family Cites Families (226)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US205178A (en) * 1878-06-25 Improvement in feather-renovators
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5036006A (en) 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5453566A (en) 1986-03-28 1995-09-26 Calgene, Inc. Antisense regulation of gene expression in plant/cells
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
NZ230375A (en) 1988-09-09 1991-07-26 Lubrizol Genetics Inc Synthetic gene encoding b. thuringiensis insecticidal protein
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
EP1103616A3 (fr) 1989-02-24 2001-06-27 Monsanto Company Gènes synthétiques de plantes et méthode pour leur préparation
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
US5231020A (en) 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5034323A (en) 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
ES2187497T3 (es) 1990-04-12 2003-06-16 Syngenta Participations Ag Promotores preferentemente en tejidos.
CA2080584C (fr) 1990-04-18 2000-09-05 Marc Cornelissen Genes modifies de la proteine des cristaux de l'insecticide a base de bacillus thuringiensis, et leur expression dans des cellules vegetales
EP0528857B1 (fr) 1990-04-26 2002-01-30 Aventis CropScience N.V. Nouvelle souche de bacillus thuringiensis et sa gene de codage de toxine insecticide
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5277905A (en) 1991-01-16 1994-01-11 Mycogen Corporation Coleopteran-active bacillus thuringiensis isolate
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
GB9114259D0 (en) 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences
CA2112676C (fr) 1991-08-02 2007-07-03 Michio Ohba Nouveaux microorganisme et insecticide
DE69230290T2 (de) 1991-08-27 2000-07-20 Novartis Ag, Basel Proteine mit insektiziden eigenschaften gegen homopteran insekten und ihre verwendung im pflanzenschutz
CA2112999C (fr) 1991-10-04 2007-04-24 Mark A. Conkling Plantes transgeniques resistantes aux pathogenes
TW261517B (fr) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
US5792632A (en) 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5474896A (en) 1992-05-05 1995-12-12 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
GB9210273D0 (en) 1992-05-13 1992-07-01 Ici Plc Dna
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
WO1994002620A2 (fr) 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. Procede ameliore de transformation induite par agrobacterium de cellules de soja cultivees
AU5676394A (en) 1992-11-20 1994-06-22 Agracetus, Inc. Transgenic cotton plants producing heterologous bioplastic
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
DE69428290T2 (de) 1993-01-13 2002-04-18 Pioneer Hi-Bred International, Inc. Derivate von alpha-hordothionin mit höherem behalt an lysin
US5527695A (en) 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
US5583210A (en) 1993-03-18 1996-12-10 Pioneer Hi-Bred International, Inc. Methods and compositions for controlling plant development
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US5593881A (en) 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5792931A (en) 1994-08-12 1998-08-11 Pioneer Hi-Bred International, Inc. Fumonisin detoxification compositions and methods
US7262055B2 (en) 1998-08-25 2007-08-28 Gendaq Limited Regulated gene expression in plants
EP0711834A3 (fr) 1994-10-14 1996-12-18 Nissan Chemical Ind Ltd Nouvelle souche de Bacillus et agents de contrÔle d'organismes nuisibles
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
PL323635A1 (en) 1995-06-02 1998-04-14 Pioneer Hi Bred Int Derivatives of alpha-hordothionine of high methionine content
BR9609200A (pt) 1995-06-02 1999-05-11 Pioneer Hi Bred Int Derivados de alta treonina de alpha-hordotionina
NZ500843A (en) 1995-06-07 2002-03-28 Invitrogen Corp A method of DNA recombination which is selectable to select for cells containing a product and against cells only harbouring the insert donor
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US5703049A (en) 1996-02-29 1997-12-30 Pioneer Hi-Bred Int'l, Inc. High methionine derivatives of α-hordothionin for pathogen-control
US5693512A (en) 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US5850016A (en) 1996-03-20 1998-12-15 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
US6175056B1 (en) 1996-04-29 2001-01-16 The Procter & Gamble Company Disposable absorbent article having an expanding topsheet and being capable of self-shaping in use
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
WO1998001575A1 (fr) 1996-07-08 1998-01-15 Pioneer Hi-Bred International, Inc. Transformation de cellules de zygote, d'oeuf et de sperme et recuperation de plantes transformees dans des sacs embryonnaires isoles
AU728086B2 (en) 1996-11-01 2001-01-04 Pioneer Hi-Bred International, Inc. Proteins with enhanced levels of essential amino acids
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
GB9710475D0 (en) 1997-05-21 1997-07-16 Zeneca Ltd Gene silencing
JP4206154B2 (ja) 1997-11-13 2009-01-07 大日本住友製薬株式会社 変異型ｌｏｘＰ配列とその応用
NZ503859A (en) 1997-11-18 2003-02-28 Pioneer Hi Bred Int Non-identical minimal recombination sites (FRT) which are non-identical for targeted integration of nucleotide sequences into transformed plants
WO1999025840A1 (fr) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Nouveau procede d'integration d'adn etranger dans des genomes .
DE69841438D1 (de) 1997-11-18 2010-02-25 Pioneer Hi Bred Int Mobilisierung eines viralen genoms aus t-dna durch ortsspezifische rekombinationssysteme
US5929301A (en) 1997-11-18 1999-07-27 Pioneer Hi-Bred International Nucleic acid sequence encoding FLP recombinase
FR2772787B1 (fr) * 1997-12-24 2001-12-07 Rhone Poulenc Agrochimie Promoteur h3c4 de mais associe au premier intron de l'actine de riz, gene chimere le comprenant et plante transformee
ATE342985T1 (de) 1998-02-26 2006-11-15 Pioneer Hi Bred Int Mais alpha-tubulin 3-18 promoter
BRPI9908967B1 (pt) 1998-03-20 2017-05-30 Benitec Australia Ltd processos para reprimir, retardar ou de outro modo reduzir a expressão de um gene alvo em uma célula de planta
AU760041B2 (en) 1998-04-08 2003-05-08 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
AR020078A1 (es) 1998-05-26 2002-04-10 Syngenta Participations Ag Metodo para alterar la expresion de un gen objetivo en una celula de planta
WO2000011155A1 (fr) * 1998-08-19 2000-03-02 The Board Of Trustees Of The Leland Stanford Junior University Procedes et compositions pour la modification de genomes
WO2000011177A1 (fr) 1998-08-20 2000-03-02 Pioneer Hi-Bred International, Inc. Promoteurs preferes des graines
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
WO2000049035A1 (fr) 1999-02-19 2000-08-24 The General Hospital Corporation Inhibition d'un gene
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
AU6364800A (en) 1999-07-23 2001-02-13 Regents Of The University Of California, The Dna recombination in eukaryotic cells by the bacteriophage phic31 recombination system
AU6529900A (en) 1999-08-09 2001-03-05 Monsanto Technology Llc Novel cloning methods and vectors
WO2001023545A1 (fr) 1999-09-30 2001-04-05 Sumitomo Pharmaceuticals Company, Limited Adn contenant des sequences frt allelomorphes
US20070083945A1 (en) 2000-03-10 2007-04-12 Byrum Joseph R Nucleic acid molecules and other molecules associated with plants
WO2001083819A2 (fr) 2000-04-28 2001-11-08 Sangamo Biosciences, Inc. Procédés d'élaboration de molécules régulatrices exogènes
US20030082561A1 (en) 2000-07-21 2003-05-01 Takashi Sera Zinc finger domain recognition code and uses thereof
US20020048814A1 (en) 2000-08-15 2002-04-25 Dna Plant Technology Corporation Methods of gene silencing using poly-dT sequences
US7612251B2 (en) 2000-09-26 2009-11-03 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
US7462481B2 (en) 2000-10-30 2008-12-09 Verdia, Inc. Glyphosate N-acetyltransferase (GAT) genes
CA2435394C (fr) 2001-01-22 2018-01-09 Sangamo Biosciences, Inc. Proteines de liaison en doigt de gant modifiees
GB0108491D0 (en) 2001-04-04 2001-05-23 Gendaq Ltd Engineering zinc fingers
DE10131786A1 (de) 2001-07-04 2003-01-16 Sungene Gmbh & Co Kgaa Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen
AT5356U1 (de) 2001-07-16 2002-06-25 Uv & P Umweltman Verfahrenstec Verfahren zur immobilisierung von verbrennungsrückständen
CN1646687A (zh) 2002-03-14 2005-07-27 联邦科学和工业研究组织 修饰的基因沉默rna及其用途
AU2003215869B2 (en) 2002-03-15 2008-04-24 Cellectis Hybrid and single chain meganucleases and use thereof
AU2003234328A1 (en) 2002-04-30 2003-11-17 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
AU2003290518A1 (en) 2002-09-06 2004-04-23 Fred Hutchinson Cancer Research Center Methods and compositions concerning designed highly-specific nucleic acid binding proteins
US6967263B2 (en) 2002-09-13 2005-11-22 Monsanto Technology, L.L.C. Soybean cultivar SN79525
AU2004208031B2 (en) 2003-01-28 2009-10-08 Cellectis Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof.
WO2004073390A1 (fr) 2003-02-19 2004-09-02 Commonwealth Scientific And Industrial Research Organisation Blocage genique efficace chez des vegetaux a l’aide de courtes sequences d’arn bicatenaire
IES20030696A2 (en) 2003-09-23 2005-03-23 Carsphairn Ltd A pipeline apparatus
ATE514782T1 (de) 2003-11-18 2011-07-15 Bayer Bioscience Nv Verbesserte gezielte dna insertion in pflanzen
US7935862B2 (en) 2003-12-02 2011-05-03 Syngenta Participations Ag Targeted integration and stacking of DNA through homologous recombination
WO2005075655A2 (fr) 2004-02-02 2005-08-18 Pioneer Hi-Bred International, Inc. Facteur de transcription odp2 (ovule development protein 2) du domaine ap2 et procedes pour l'utiliser
EP1591521A1 (fr) 2004-04-30 2005-11-02 Cellectis Dérivés de I-Dmo I ayant une activité améliorée à 37degrés C et leur utilisation
US20070199095A1 (en) 2005-10-13 2007-08-23 Edwards Allen Methods for producing hybrid seed
WO2006097784A1 (fr) 2005-03-15 2006-09-21 Cellectis Variants de meganuclease i-crei presentant une specificite modifiee, leur procede de preparation, et leurs utilisations
AU2006224248B2 (en) 2005-03-15 2011-01-06 Cellectis I-Crei meganuclease variants with modified specificity, method of preparation and uses thereof
AP2693A (en) * 2005-05-27 2013-07-16 Monsanto Technology Llc Soybean event MON89788 and methods for detection thereof
EP1907553B1 (fr) 2005-07-18 2012-08-22 Pioneer Hi-Bred International Inc. Sites de recombinaison frt modifies et leurs procedes d'utilisation
US20070016985A1 (en) 2005-07-18 2007-01-18 Pioneer Hi-Bred International, Inc. Particle Preparation for Direct-Delivery Transformation
NZ597299A (en) 2005-08-26 2013-03-28 Dupont Nutrition Biosci Aps Use of a cas gene in combination with CRISPR repeats for modulating resistance in a cell
EP2650366B1 (fr) 2005-10-18 2017-03-22 Precision Biosciences Meganucleases concues rationnellement possedant une specificité sequence modifiée et une affinité de liaison pour l'ADN
WO2011066384A1 (fr) 2009-11-24 2011-06-03 Dow Agrosciences Llc Événement 416 de la transformation aad-12, lignées de soja transgéniques associées, et leur identification spécifique à l'événement
CN101484581A (zh) 2006-05-09 2009-07-15 密苏里大学学监 通过端粒截短的植物人工染色体平台
WO2007134122A2 (fr) 2006-05-09 2007-11-22 The Curators Of The University Of Missouri Plateformes végétales de chromosomes artificiels au moyen d'un troncage du télomère
US7951995B2 (en) 2006-06-28 2011-05-31 Pioneer Hi-Bred International, Inc. Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof
US7968770B2 (en) 2006-06-28 2011-06-28 Pioneer Hi-Bred International, Inc. Methods for improving yield using soybean event 3560.4.3.5
EP2078089B1 (fr) 2006-10-31 2016-05-04 E. I. du Pont de Nemours and Company Événement de soja dp-305423-1, leurs compositions et leurs procédés d'identification et/ou de détection
WO2008083233A2 (fr) 2006-12-29 2008-07-10 Dow Agrosciences Llc Procédés in vitro pour l'induction et la maintenance de lignées cellulaires végétales sous forme de cellules en suspension uniques avec des parois cellulaires intactes, et la transformation de celles-ci
WO2008148223A1 (fr) 2007-06-07 2008-12-11 Agriculture And Agri-Food Canada Transfection et transduction végétale basée sur un nanosupport
BRPI0817911B8 (pt) 2007-10-05 2022-06-28 Dow Agrosciences Llc Processos para transferência de substâncias moleculares para dentro de células vegetais e processo para expressão de um gene
WO2010011961A2 (fr) 2008-07-25 2010-01-28 University Of Georgia Research Foundation, Inc. Système procaryote arni-like et ses procédés d'utilisation
US20100076057A1 (en) 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
US8293533B2 (en) 2008-12-19 2012-10-23 E.I. Du Pont De Nemours And Company Site-specific integration and stacking of transgenes in soybean via DNA recombinase mediated cassette exchange
EP2206723A1 (fr) 2009-01-12 2010-07-14 Bonas, Ulla Domaines modulaires de liaison à l'ADN
WO2010083178A1 (fr) 2009-01-16 2010-07-22 Monsanto Technology Llc Nouvelles molécules d'acide nucléique et de protéine isolées issues de maïs et procédés d'utilisation de ces molécules pour produire des plantes transgéniques présentant des caractères agronomiques améliorés
EP2417262B1 (fr) 2009-04-07 2015-05-20 Dow AgroSciences LLC Distribution à médiation par nanoparticules de nucléases à spécificité de séquence
US8704041B2 (en) 2009-12-30 2014-04-22 Pioneer Hi Bred International Inc Methods and compositions for targeted polynucleotide modification
US20110203012A1 (en) 2010-01-21 2011-08-18 Dotson Stanton B Methods and compositions for use of directed recombination in plant breeding
CA2787674C (fr) 2010-01-22 2022-09-06 Dow Agrosciences Llc Excision de transgenes dans organismes genetiquement modifies
GEP201606587B (en) 2010-01-22 2017-01-10 Dow Agrosciences Llc Engineered landing pads for gene targeting in plants
JP5902631B2 (ja) 2010-01-22 2016-04-13 ダウ アグロサイエンシィズ エルエルシー 標的化ゲノム変更
EP2552191B1 (fr) 2010-03-31 2020-11-04 Dow AgroSciences, LLC Peptide de gamma-zéine végétale pour la délivrance de biomolécules dans des cellules végétales
EA024121B9 (ru) 2010-05-10 2017-01-30 Дзе Реджентс Ов Дзе Юниверсити Ов Калифорния Композиции эндорибонуклеаз и способы их использования
KR20130124296A (ko) 2010-07-07 2013-11-13 다우 아그로사이언시즈 엘엘씨 기능성화된 선형 ｄｎａ 카세트의 생산 및 식물 내에서 양자점/나노입자 매개된 전달
CA2804168C (fr) 2010-07-07 2020-02-18 Dow Agrosciences Llc Delivrance d'une molecule d'adn lineaire utilisant des boites quantiques pegylees pour une transformation stable dans des plantes
CA2830531C (fr) 2011-03-23 2019-10-15 Dow Agrosciences Llc Conjugues peptidiques supports de points quantiques adaptes pour des applications d'imagerie et d'administration dans des plantes
EP2689019A2 (fr) 2011-03-23 2014-01-29 Pioneer Hi-Bred International Inc. Procédés de production d'un locus complexe de caractéristique transgénique
WO2012164565A1 (fr) 2011-06-01 2012-12-06 Yeda Research And Development Co. Ltd. Compositions et procédés pour la régulation à la baisse de gènes procaryotes
AR086995A1 (es) 2011-06-21 2014-02-05 Pioneer Hi Bred Int Metodos y composiciones para producir plantas con esterilidad masculina
US9951346B2 (en) 2011-08-03 2018-04-24 Pioneer Hi-Bred International, Inc. Methods and compositions for targeted integration in a plant
AU2012299691B2 (en) 2011-08-22 2015-01-29 BASF Agricultural Solutions Seed US LLC Methods and means to modify a plant genome
EP2776560A2 (fr) 2011-11-07 2014-09-17 The University of Western Ontario Endonucléase pour l'édition de génome
US20130157369A1 (en) 2011-12-15 2013-06-20 Dow Agrosciences Llc Method for improved transformation using agrobacterium
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
AR089793A1 (es) 2012-01-27 2014-09-17 Du Pont Metodos y composiciones para generar locus de rasgos transgenicos complejos
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
WO2013141680A1 (fr) 2012-03-20 2013-09-26 Vilnius University Clivage d'adn dirigé par arn par le complexe cas9-arncr
JP6559063B2 (ja) 2012-05-07 2019-08-14 サンガモ セラピューティクス， インコーポレイテッド 導入遺伝子のヌクレアーゼ媒介標的化組み込みのための方法および組成物
RU2014151361A (ru) 2012-05-18 2016-07-10 Пайонир Хай-Бред Интернэшнл, Инк. Последовательности индуцируемого промотора для регулируемой экспрессии и способы применения
LT3401400T (lt) 2012-05-25 2019-06-10 The Regents Of The University Of California Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui
JP2015527889A (ja) 2012-07-25 2015-09-24 ザ ブロード インスティテュート， インコーポレイテッド 誘導可能なｄｎａ結合タンパク質およびゲノム撹乱ツール、ならびにそれらの適用
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
US20140096284A1 (en) 2012-10-01 2014-04-03 Iowa State University Research Foundation, Inc. Method for the delivery of molecules lyophilized onto microparticles to plant tissues
PL4397760T3 (pl) 2012-10-23 2026-03-30 Toolgen Incorporated Kompozycja do rozszczepiania docelowego dna zawierająca prowadzące rna specyficzne dla docelowego dna i kwas nukleinowy kodujący białko cas lub białko cas oraz jej zastosowanie
US20150291967A1 (en) 2012-10-31 2015-10-15 Luc Mathis Coupling herbicide resistance with targeted insertion of transgenes in plants
EP3135765A1 (fr) 2012-12-06 2017-03-01 Sigma-Aldrich Co. LLC Modification et régulation de génome à base de crispr
WO2014093479A1 (fr) 2012-12-11 2014-06-19 Montana State University Contrôle de la régulation génétique guidé par arn crispr (répétitions palindromiques groupées, courtes régulièrement espacées )
CN114634950A (zh) 2012-12-12 2022-06-17 布罗德研究所有限公司 用于序列操纵的crispr-cas组分系统、方法以及组合物
EP2931898B1 (fr) 2012-12-12 2016-03-09 The Broad Institute, Inc. Fabrication et optimisation de systèmes, de procédés et de compositions pour la manipulation de séquence avec des domaines fonctionnels
WO2014093701A1 (fr) 2012-12-12 2014-06-19 The Broad Institute, Inc. Génomique fonctionnelle employant des systèmes crispr-cas, des compositions, des procédés, des banques d'inactivation et leurs applications
ES2576126T3 (es) 2012-12-12 2016-07-05 The Broad Institute, Inc. Modificación por tecnología genética y optimización de sistemas, métodos y composiciones enzimáticas mejorados para la manipulación de secuencias
IL239317B (en) 2012-12-12 2022-07-01 Broad Inst Inc Providing, engineering and optimizing systems, methods and compositions for sequence manipulation and therapeutic applications
EP2896697B1 (fr) 2012-12-12 2015-09-02 The Broad Institute, Inc. Ingénierie de systèmes, procédés et compositions de guidage optimisé pour manipulation de séquence
JP2016504026A (ja) 2012-12-12 2016-02-12 ザ・ブロード・インスティテュート・インコーポレイテッド 配列操作のための系、方法および最適化ガイド組成物のエンジニアリング
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
US20140173783A1 (en) 2012-12-13 2014-06-19 Dow Agrosciences Llc Precision gene targeting to a particular locus in maize
SG10201912991WA (en) 2012-12-17 2020-03-30 Harvard College Rna-guided human genome engineering
EP2958996B1 (fr) 2013-02-25 2019-10-16 Sangamo Therapeutics, Inc. Méthodes et compositions pour améliorer une disruption génique à médiation nucléase
UA123532C2 (uk) 2013-03-12 2021-04-21 Е. І. Дю Пон Де Немур Енд Компані Спосіб ідентифікації варіантного сайта розпізнавання для сконструйованого засобу, що рідко розщеплює, для індукції двониткового розриву
SG11201507378UA (en) 2013-03-14 2015-10-29 Caribou Biosciences Inc Compositions and methods of nucleic acid-targeting nucleic acids
US10378027B2 (en) 2013-03-15 2019-08-13 The General Hospital Corporation RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci
US10113162B2 (en) 2013-03-15 2018-10-30 Cellectis Modifying soybean oil composition through targeted knockout of the FAD2-1A/1B genes
US20140273235A1 (en) 2013-03-15 2014-09-18 Regents Of The University Of Minnesota ENGINEERING PLANT GENOMES USING CRISPR/Cas SYSTEMS
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
JP2016522679A (ja) 2013-04-04 2016-08-04 プレジデント アンド フェローズ オブ ハーバード カレッジ ＣＲＩＳＰＲ／Ｃａｓ系を用いたゲノム編集の治療的使用
WO2014186686A2 (fr) 2013-05-17 2014-11-20 Two Blades Foundation Mutagénèse ciblée et ingénierie génomique dans des plantes à l'aide de nucléases cas guidées par arn
WO2014194190A1 (fr) 2013-05-30 2014-12-04 The Penn State Research Foundation Ciblage génique et modification génétique de végétaux par le biais de l'édition du génome guidée par l'arn
BR112016000571B1 (pt) 2013-07-10 2023-12-26 President And Fellows Of Harvard College Métodos in vitro para modular a expressão e para alterar um ou mais ácidos nucleicos alvo em uma célula simultaneamente com a regulação da expressão de um ou mais ácidos nucleicos alvo em uma célula, bem como célula de levedura ou bactéria compreendendo ácidos nucleicos
US20150044772A1 (en) 2013-08-09 2015-02-12 Sage Labs, Inc. Crispr/cas system-based novel fusion protein and its applications in genome editing
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
CA3221516A1 (fr) 2013-08-22 2015-02-26 E. I. Du Pont De Nemours And Company Modification du genome des plantes a l'aide de systemes d'arn de guida /endonuclease cas et leurs procedes d'utilisation
CA2916060A1 (fr) 2013-08-27 2015-03-05 Evogene Ltd. Polynucleotides et polypeptides isoles, et procedes les utilisant pour augmenter un rendement vegetal et/ou des caracteristiques agricoles
CN105682452B (zh) 2013-09-04 2018-10-16 美国陶氏益农公司 用于在作物中确定供体插入的快速靶向分析
KR102269769B1 (ko) 2013-11-04 2021-06-28 코르테바 애그리사이언스 엘엘씨 최적 메이즈 유전자좌
US10077449B2 (en) 2013-11-04 2018-09-18 Dow Agrosciences Llc Universal donor system for gene targeting
MX358066B (es) 2013-11-04 2018-08-03 Dow Agrosciences Llc Óptimos loci de soya.
UY35814A (es) 2013-11-04 2015-05-29 Dow Agrosciences Llc ?lugares óptimos para la soja?.
BR102014027442B1 (pt) 2013-11-04 2022-09-27 Dow Agrosciences Llc Molécula de ácido nucleico recombinante, uso de uma planta de milho, parte de planta de milho ou célula de planta de milho compreendendo a mesma e método para produzir uma célula vegetal transgênica compreendendo um dna de interesse
CA2930877A1 (fr) 2013-11-18 2015-05-21 Crispr Therapeutics Ag Systeme crips-cas, materiels et procedes
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
JP2017503514A (ja) 2014-01-24 2017-02-02 ノースカロライナ ステート ユニバーシティーＮｏｒｔｈ Ｃａｒｏｌｉｎａ Ｓｔａｔｅ Ｕｎｉｖｅｒｓｉｔｙ Ｃａｓ９ターゲッティングをガイドする配列に関する方法および組成物
EP3971283A1 (fr) 2014-02-27 2022-03-23 Monsanto Technology LLC Compositions et procédés de modification génomique dirigée
US11584936B2 (en) 2014-06-12 2023-02-21 King Abdullah University Of Science And Technology Targeted viral-mediated plant genome editing using CRISPR /Cas9
CN106687594A (zh) 2014-07-11 2017-05-17 纳幕尔杜邦公司 用于产生对草甘膦除草剂具有抗性的植物的组合物和方法
WO2016007948A1 (fr) 2014-07-11 2016-01-14 Pioneer Hi-Bred International, Inc. Modification de caractère agronomique au moyen d'arn guide/endonucléase cas, systèmes et procédés d'utilisation
US10450584B2 (en) 2014-08-28 2019-10-22 North Carolina State University Cas9 proteins and guiding features for DNA targeting and genome editing
BR112017003757A2 (pt) 2014-09-12 2017-12-26 Du Pont ?plantas de milho, partes de planta de milho ou sementes de milho?
SI3250691T1 (sl) 2015-01-28 2023-10-30 Caribou Biosciences, Inc. Hibridni dna/rna-polinukleotidi crispr in postopki za uporabo
EP3262176A1 (fr) 2015-02-25 2018-01-03 Pioneer Hi-Bred International, Inc. Composition et procédés pour la régulation de l'expression d'un complexe arn guide/endonucléase cas
WO2016149352A1 (fr) 2015-03-19 2016-09-22 Pioneer Hi-Bred International Inc Procédés et compositions permettant d'accélérer l'introgression de caractères
EP3553178A1 (fr) 2015-03-27 2019-10-16 E. I. du Pont de Nemours and Company Promoteurs de petit gène d'arn nucléaire u6 de graines de soja et leur utilisation dans l'expression de constitutive de petits gènes dans des plantes
EP3294878A1 (fr) 2015-05-15 2018-03-21 Pioneer Hi-Bred International, Inc. Systèmes arn guide/endonucléase cas
DE102015006335A1 (de) 2015-05-19 2016-11-24 Kws Saat Se Verfahren und Konstrukte zur gezielten Nukleinsäure Editierung in Pflanzen
US10883103B2 (en) 2015-06-02 2021-01-05 Monsanto Technology Llc Compositions and methods for delivery of a polynucleotide into a plant
WO2017015015A1 (fr) 2015-07-17 2017-01-26 Emory University Protéine associée à crispr issue de francisella, et utilisations associées
CN114606226A (zh) 2015-08-21 2022-06-10 孟山都技术公司 基因组基因座的增强的重组
US11692182B2 (en) 2015-10-09 2023-07-04 Monsanto Technology Llc RNA-guided DNA nucleases and uses thereof
EP4089175A1 (fr) 2015-10-13 2022-11-16 Duke University Ingénierie génomique avec systèmes crispr de type i dans des cellules eucaryotes
CN108138155A (zh) 2015-10-20 2018-06-08 先锋国际良种公司 经由指导cas系统恢复非功能性基因产物的功能及使用方法
US20180258438A1 (en) 2015-11-06 2018-09-13 Pioneer Hi-Bred International, Inc. Generation of complex trait loci in soybean and methods of use
CN115216459B (zh) 2015-12-29 2024-06-28 孟山都技术公司 新型crispr相关转座酶及其用途
WO2017155715A1 (fr) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Nouveaux systèmes cas9 et procédés d'utilisation
CA3010628A1 (fr) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Nouveaux systemes cas9 et procedes d'utilisation
EP3426780A1 (fr) 2016-03-11 2019-01-16 Pioneer Hi-Bred International, Inc. Nouveaux systèmes cas9 et procédés d'utilisation
GB201610041D0 (en) 2016-06-08 2016-07-20 Oxford Genetics Ltd Methods
MX2018014993A (es) 2016-06-14 2019-09-06 Pioneer Hi Bred Int Uso de endonucleasa cpf1 para modificaciones de genoma de planta.
WO2017222773A1 (fr) 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Nouveaux systèmes cas et méthodes d'utilisation
CN110914433A (zh) 2017-03-24 2020-03-24 库尔维科公司 编码crispr相关蛋白质的核酸及其用途

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008148559A1 (fr) * 2007-06-05 2008-12-11 Bayer Bioscience N.V. Procédés et moyens permettant le remplacement précis d'un adn cible dans des organismes eucaryotes
WO2009006297A2 (fr) * 2007-06-29 2009-01-08 Pioneer Hi-Bred International, Inc. Procédés de modification du génome d'une cellule de plante monocotylédone
WO2009042164A1 (fr) * 2007-09-27 2009-04-02 Dow Agrosciences Llc Protéines à doigt de zinc synthétisées ciblant des gènes de 5-énolpyruvyl shikimate-3-phosphate synthase
WO2009114321A2 (fr) * 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Méganucléases rationnellement conçues pour modification par génie génétique du génome du maïs
WO2010077319A1 (fr) * 2008-12-17 2010-07-08 Dow Agrosciences Llc Intégration ciblée dans le locus zp15
WO2011117249A1 (fr) * 2010-03-22 2011-09-29 Philip Morris Products S.A. Modification d'activité enzymatique dans des plantes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
QUE Q., ET AL.: "Trait stacking in transgenic crops: challenges and opportunities", GM CROPS, vol. 1, no. 4, October 2010 (2010-10-01), pages 220 - 229, XP002677400 *
TOWNSEND JEFFREY A ET AL: "High-frequency modification of plant genes using engineered zinc-finger nucleases", NATURE: INTERNATIONAL WEEKLY JOURNAL OF SCIENCE, NATURE PUBLISHING GROUP, UNITED KINGDOM, vol. 459, no. 7245, 21 May 2009 (2009-05-21), pages 442 - 445, XP002588972, ISSN: 0028-0836, DOI: 10.1038/NATURE07845 *

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US20180305706A1 (en) 2018-10-25
US11459576B2 (en) 2022-10-04
WO2012129373A2 (fr) 2012-09-27
CA2831144A1 (fr) 2012-09-27
CN103842511A (zh) 2014-06-04
US10030245B2 (en) 2018-07-24
US20250277227A1 (en) 2025-09-04
US20230235342A1 (en) 2023-07-27
US20130263324A1 (en) 2013-10-03
BR112013024337A2 (pt) 2017-09-26
MX2013010911A (es) 2015-03-03
US12338444B2 (en) 2025-06-24
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US10822610B2 (en) 2020-11-03
US20210062201A1 (en) 2021-03-04
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