ZA200605588B - Gene expression technique - Google Patents

Gene expression technique

Info

Publication number
ZA200605588B
ZA200605588B ZA200605588A ZA200605588A ZA200605588B ZA 200605588 B ZA200605588 B ZA 200605588B ZA 200605588 A ZA200605588 A ZA 200605588A ZA 200605588 A ZA200605588 A ZA 200605588A ZA 200605588 B ZA200605588 B ZA 200605588B
Authority
ZA
South Africa
Prior art keywords
protein
gene encoding
host cell
heterologous protein
gene expression
Prior art date
Application number
ZA200605588A
Other languages
English (en)
Inventor
Sleep Darrell
Shuttleworth Gillian
Finnis Christopher John Arthur
Original Assignee
Delta Biotechnology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Delta Biotechnology Ltd filed Critical Delta Biotechnology Ltd
Publication of ZA200605588B publication Critical patent/ZA200605588B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ZA200605588A 2003-12-23 2004-12-23 Gene expression technique ZA200605588B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0329681.1A GB0329681D0 (en) 2003-12-23 2003-12-23 Gene expression technique

Publications (1)

Publication Number Publication Date
ZA200605588B true ZA200605588B (en) 2009-02-25

Family

ID=30776264

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200605588A ZA200605588B (en) 2003-12-23 2004-12-23 Gene expression technique

Country Status (15)

Country Link
US (3) US20070275889A1 (de)
EP (2) EP1709181B1 (de)
JP (1) JP5052899B2 (de)
KR (1) KR101262682B1 (de)
CN (2) CN1922325B (de)
AT (1) ATE421994T1 (de)
AU (2) AU2004303601C1 (de)
CA (1) CA2551503A1 (de)
DE (1) DE602004019304D1 (de)
DK (1) DK2048236T3 (de)
ES (1) ES2321948T3 (de)
GB (2) GB0329681D0 (de)
SG (2) SG182155A1 (de)
WO (1) WO2005061718A1 (de)
ZA (1) ZA200605588B (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003244515A1 (en) * 2002-02-07 2003-09-02 Novozymes Delta Limited Albumin-fused anti-angiogenesis peptides
GB0329722D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
DK2330200T3 (en) 2004-12-23 2017-07-24 Albumedix As GENE EXPRESSION TECHNIQUE
GB0512707D0 (en) * 2005-06-22 2005-07-27 Delta Biotechnology Ltd Gene expression technique
WO2008012629A2 (en) 2006-07-24 2008-01-31 Biorexis Pharmaceutical Corporation Exendin fusion proteins
GB0711424D0 (en) * 2007-06-13 2007-07-25 Novozymes Delta Ltd Recombinant transferrin mutants
CN101835801B (zh) 2007-08-08 2014-09-10 诺维信生物制药丹麦公司 转铁蛋白变体和偶联物
JP5288379B2 (ja) † 2007-10-31 2013-09-11 独立行政法人産業技術総合研究所 タンパク質の高分泌生産方法
EP4219547A3 (de) 2008-09-15 2023-10-18 uniQure biopharma B.V. Faktor-ix-polypeptidmutante, deren verwendungen und verfahren zu deren herstellung
WO2010034708A1 (en) * 2008-09-23 2010-04-01 Unilever Plc A polynucleotide expression cassette
KR101722961B1 (ko) 2009-02-11 2017-04-04 알부메딕스 에이/에스 알부민 변이체 및 접합체
DK2427486T3 (en) 2009-05-07 2015-05-26 Novozymes Biopharma Dk As A process for purifying albumin
EP2258854A1 (de) 2009-05-20 2010-12-08 FH Campus Wien Eukaryotische Wirtszelle mit einem Ausdrucksverstärker
FI20095615A0 (fi) 2009-06-02 2009-06-02 Oulun Yliopisto Menetelmä luonnollisesti laskostuneiden proteiinien tuottamiseksi prokaryootti-isännässä
CA2776241A1 (en) 2009-10-30 2011-05-05 Novozymes Biopharma Dk A/S Albumin variants
CN103641896B (zh) * 2009-11-19 2015-08-05 浙江大学 明胶样单元的用途
GB201000587D0 (en) * 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
US10233228B2 (en) 2010-04-09 2019-03-19 Albumedix Ltd Albumin derivatives and variants
CN102533839B (zh) * 2010-12-21 2017-06-20 上海开阳生物科技有限公司 融合表达含有二硫键蛋白质的方法
EP2565262A1 (de) * 2011-08-31 2013-03-06 VTU Holding GmbH Protein-Expression
EP2780364A2 (de) 2011-11-18 2014-09-24 Eleven Biotherapeutics, Inc. Proteine mit verbesserter halbwertzeit und anderen eigenschaften
WO2013135896A1 (en) 2012-03-16 2013-09-19 Novozymes Biopharma Dk A/S Albumin variants
MX2015005363A (es) 2012-11-08 2015-11-06 Novozymes Biopharma Dk As Variantes de albumina.
CN102994541B (zh) * 2012-12-19 2015-04-15 江南大学 一种共表达upr关键基因和下游靶基因增强葡萄糖氧化酶分泌的方法
CN104937599B (zh) * 2013-02-28 2018-01-23 株式会社日立高新技术 数据解析装置及其方法
CN103436516B (zh) * 2013-08-23 2015-04-22 华南理工大学 利用大肠杆菌原核表达系统生产的小麦蛋白质二硫键异构酶及方法和应用
BR112016023304A2 (pt) 2014-04-17 2017-10-17 Boehringer Ingelheim Rcv Gmbh célula hospedeira recombinante engendrada para superexpressar proteínas auxiliares
EP3132039B1 (de) 2014-04-17 2019-07-24 Boehringer Ingelheim RCV GmbH & Co KG Rekombinante wirtszelle zur expression von zielproteinen
WO2017029407A1 (en) 2015-08-20 2017-02-23 Albumedix A/S Albumin variants and conjugates
EP3394243A1 (de) 2015-12-22 2018-10-31 Albumedix Ltd. Verbesserte proteinexpressionsstämme
JP7146637B2 (ja) 2015-12-22 2022-10-04 アルブミディクス リミティド 改善されたタンパク質発現株
EP3453763B1 (de) * 2016-05-04 2021-12-22 Shenzhen Protgen Ltd. Konstruktion von genetischen modifizierten hefen pichia pastoris zur hohen expression von rekombinantem menschlichem serumalbumin
EP3568410A4 (de) 2017-01-16 2020-12-23 Nansha Biologics (Hong Kong) Limited Systeme und verfahren zur herstellung von rekombinanter il-11-in-hefe
CN106834322B (zh) * 2017-03-14 2020-09-04 中国科学院南海海洋研究所 抗毒素so_1445或其编码基因在提高质粒在宿主细胞中的稳定性中的应用
JP7282693B2 (ja) 2017-06-20 2023-05-29 アルブミディクス リミティド 改良されたタンパク質発現株
WO2019006348A1 (en) * 2017-06-30 2019-01-03 Western University Of Health Sciences IX-TRANSFERRIN FACTOR FUSION PROTEINS
EP3775222B1 (de) 2018-03-26 2024-01-03 Novozymes A/S Chaperon-proteine aus pilzen
CN112391402B (zh) * 2020-11-17 2023-03-28 华中科技大学 一种提高解脂耶氏酵母中目的蛋白表达水平的方法
WO2022191252A1 (ja) * 2021-03-10 2022-09-15 住友ファーマ株式会社 システインノットタンパク質の製造方法
CN114410496B (zh) * 2022-02-16 2023-10-03 江南大学 一种提高毕赤酵母外源蛋白产量的方法
KR20240094203A (ko) * 2022-12-15 2024-06-25 씨제이제일제당 (주) 트리코더마 속 균주 유래 pdi를 이용하여 재조합 단백질을 생산하는 방법
CN117903295B (zh) * 2024-03-19 2024-05-31 北京国科星联科技有限公司 一种用于分泌表达乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用
CN118460394B (zh) * 2024-07-12 2024-09-24 通化安睿特生物制药股份有限公司 一种用于制备转铁蛋白的工程菌及其应用
CN118895293B (zh) * 2024-10-08 2025-01-28 通化安睿特生物制药股份有限公司 一种产人白蛋白的重组工程菌及其构建方法和应用

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9019919D0 (en) 1990-09-12 1990-10-24 Delta Biotechnology Ltd Purification of proteins
US4302386A (en) * 1978-08-25 1981-11-24 The Ohio State University Antigenic modification of polypeptides
NZ199722A (en) 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
IL66614A (en) 1981-08-28 1985-09-29 Genentech Inc Method of constructing a dna sequence encoding a polypeptide,microbial production of human serum albumin,and pharmaceutical compositions comprising it
CA1205935A (en) 1981-09-14 1986-06-10 Union Carbide Corporation Process for producing a polymer water-in-oil emulsion
GB8615701D0 (en) * 1986-06-27 1986-08-06 Delta Biotechnology Ltd Stable gene integration vector
GB8620926D0 (en) 1986-08-29 1986-10-08 Delta Biotechnology Ltd Yeast promoter
WO1988008027A1 (en) * 1987-04-09 1988-10-20 Delta Biotechnology Limited Yeast vector
IL86455A0 (en) 1987-06-01 1988-11-15 Takeda Chemical Industries Ltd Polypeptide and production thereof
IL88326A (en) 1987-11-18 1993-03-15 Gist Brocades Nv Purification of serum albumin
WO1990001063A1 (en) 1988-07-23 1990-02-08 Delta Biotechnology Limited New secretory leader sequences
FR2649991B2 (fr) * 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces
US5759802A (en) 1988-10-26 1998-06-02 Tonen Corporation Production of human serum alubumin A
GB8909916D0 (en) 1989-04-29 1989-06-14 Delta Biotechnology Ltd Polypeptides
US5571691A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Production of recombinant lactoferrin and lactoferrin polypeptides using CDNA sequences in various organisms
GB8927480D0 (en) 1989-12-05 1990-02-07 Delta Biotechnology Ltd Mutant fungal strain detection and new promoter
JP3230091B2 (ja) 1990-06-25 2001-11-19 ウェルファイド株式会社 ヒト血清アルブミンの着色抑制方法
DE69114500T2 (de) * 1990-07-13 1996-06-13 Transkaryotic Therapies Inc Verfahren zum absuchen einer bibliothek.
EP0509841A3 (en) 1991-04-18 1993-08-18 Tonen Corporation Co-expression system of protein disulfide isomerase gene and useful polypeptide gene and process for producing the polypeptide using its system
US6291205B1 (en) 1992-06-12 2001-09-18 Merck & Co., Inc. Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae
WO1994003617A1 (en) * 1992-07-29 1994-02-17 Unilever N.V. Process for producing anti-freeze peptides
DE4244915C2 (de) 1992-08-14 1998-12-03 Widmar Prof Dr Tanner DNA-Moleküle codierend Proteine, die an der O-Glykosylierung von Proteinen beteiligt sind
US5728553A (en) 1992-09-23 1998-03-17 Delta Biotechnology Limited High purity albumin and method of producing
EP0663010B1 (de) * 1992-10-02 2000-08-09 Research Corporation Technologies, Inc. Methoden der erhöhung der sekretion von überexpremierten proteinen
GB9404270D0 (en) 1994-03-05 1994-04-20 Delta Biotechnology Ltd Yeast strains and modified albumins
GB9411356D0 (en) 1994-06-07 1994-07-27 Delta Biotechnology Ltd Yeast strains
JP3945866B2 (ja) * 1996-09-04 2007-07-18 サントリー株式会社 メタノール酵母由来のプロテインジスルフィドイソメラーゼ遺伝子
EP1009836A1 (de) * 1997-09-05 2000-06-21 Pentapharm A.G. Expressionsvektor für verbesserte herstellung von polypeptide in hefe
CN1111602C (zh) * 1998-05-15 2003-06-18 中国科学院上海生物化学研究所 一种使用分子伴侣促进蛋白质分泌的方法
GB9902000D0 (en) 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
EP1077263A1 (de) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
EP2206720A1 (de) 2000-04-12 2010-07-14 Human Genome Sciences, Inc. Albuminfusionsproteine
US6358733B1 (en) * 2000-05-19 2002-03-19 Apolife, Inc. Expression of heterologous multi-domain proteins in yeast
DE10121235A1 (de) * 2001-04-30 2002-10-31 Roche Diagnostics Gmbh Verfahren zur Expression von Proteinen in in vitro Translations-Systemen unter Ko-Expression von Faltungshelferproteinen
US20030226155A1 (en) 2001-08-30 2003-12-04 Biorexis Pharmaceutical Corporation Modified transferrin-antibody fusion proteins
US7176278B2 (en) 2001-08-30 2007-02-13 Biorexis Technology, Inc. Modified transferrin fusion proteins
DE10145694A1 (de) * 2001-09-17 2003-04-03 Roche Diagnostics Gmbh Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung
WO2003057897A2 (en) * 2002-01-07 2003-07-17 European Molecular Biology Laboratory Recombinant protein expression
AU2003244515A1 (en) 2002-02-07 2003-09-02 Novozymes Delta Limited Albumin-fused anti-angiogenesis peptides
EP1529108A2 (de) 2002-08-07 2005-05-11 ZLB Behring GmbH Albumin-fusionierter ciliar-neurotrophischer faktor
US6939676B2 (en) * 2002-12-30 2005-09-06 The United States Of America As Represented By The Secretary Of Argriculture Selection procedure for identifying transgenic cells, embryos, and plants without the use of antibiotics
US7244616B2 (en) * 2003-06-27 2007-07-17 Bayer Pharmaceuticals Corporation Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
US7226781B1 (en) * 2003-07-24 2007-06-05 Belyaev Alexander S Chaperone expression genomes
US8603824B2 (en) * 2004-07-26 2013-12-10 Pfenex, Inc. Process for improved protein expression by strain engineering

Also Published As

Publication number Publication date
CN1922325A (zh) 2007-02-28
AU2004303601A1 (en) 2005-07-07
CN1922325B (zh) 2010-06-09
US20120231503A1 (en) 2012-09-13
EP2048236B1 (de) 2013-03-20
US20180105818A1 (en) 2018-04-19
US20070275889A1 (en) 2007-11-29
CN101818121A (zh) 2010-09-01
JP2007515962A (ja) 2007-06-21
GB0329681D0 (en) 2004-01-28
GB0614614D0 (en) 2006-08-30
EP1709181B1 (de) 2009-01-28
AU2004303601C1 (en) 2009-01-08
SG182155A1 (en) 2012-07-30
ES2321948T3 (es) 2009-06-15
EP1709181A1 (de) 2006-10-11
GB2424889A (en) 2006-10-11
WO2005061718A1 (en) 2005-07-07
ATE421994T1 (de) 2009-02-15
DE602004019304D1 (de) 2009-03-19
AU2008202538A1 (en) 2008-06-26
KR101262682B1 (ko) 2013-05-15
EP2048236A1 (de) 2009-04-15
CA2551503A1 (en) 2005-07-07
JP5052899B2 (ja) 2012-10-17
DK2048236T3 (da) 2013-05-21
AU2004303601B2 (en) 2008-03-06
SG149003A1 (en) 2009-01-29
HK1099938A1 (en) 2007-08-31
KR20070005568A (ko) 2007-01-10

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