AR044117A1 - Fosfolipasa y metodo para producirla - Google Patents
Fosfolipasa y metodo para producirlaInfo
- Publication number
- AR044117A1 AR044117A1 ARP040101441A ARP040101441A AR044117A1 AR 044117 A1 AR044117 A1 AR 044117A1 AR P040101441 A ARP040101441 A AR P040101441A AR P040101441 A ARP040101441 A AR P040101441A AR 044117 A1 AR044117 A1 AR 044117A1
- Authority
- AR
- Argentina
- Prior art keywords
- amino acid
- seq
- phospholipase
- peptide
- amino acids
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
- A21D8/00—Methods for preparing or baking dough
- A21D8/02—Methods for preparing dough; Treating dough prior to baking
- A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
- A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
- A23C19/00—Cheese; Cheese preparations; Making thereof
- A23C19/02—Making cheese curd
- A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
- A23C19/0328—Enzymes other than milk clotting enzymes, e.g. lipase, beta-galactosidase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Polymers & Plastics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Dairy Products (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Detergent Compositions (AREA)
- Fats And Perfumes (AREA)
Abstract
Reivindicación 1: Un método para producir una fosfolipasa que comprende procesar un péptido fungal expresado para disociarlo de un péptido del extremo C-terminal y/o un péptido del extremo N-terminal para obtener un péptido de núcleo con actividad de fosfolipasa donde el péptido de núcleo comprende: a) la secuencia de aminoácido proporcionada por los aminoácidos 146-153 de la SEC ID NO: 1, los aminoácidos 87-94 de la SEC ID NO: 3, o los aminoácidos 79-86 de la SEC ID NO: 12 o una secuencia idéntica a cualquiera de estas secuencias de aminoácido excepto por la sustitución de un solo aminoácido con otro aminoácido; y b) por lo menos dos residuos cisteína situados en el lado N-terminal de la secuencia proporcionada en a); y c) por lo menos dos residuos cisteína situados en el lado C-terminal de la secuencia proporcionada en a). Reivindicación 3: El método de la reivindicación 2 donde la célula huésped es una célula de Aspergillus, Fusarium o Trichoderma, particularmente A. oryzae, A. N+iger, F. venenatum o T. reesei. Reivindicación 7: El método de cualquiera de las reivindicaciones 1-6 donde el péptido expresado deriva de Tuber, Verticillium, Neurospora, Aspergillus, o Helicosporum, particularmente, T. borchii, T. albidum, V. dahliae, V. tenerum, N. crassa, A. oryzae, o Helicosporium sp.NH1. Reivindicación 12: Un método para producir queso que comprende poner en contacto la leche de queso o una fracción de leche de queso con una fosfolipasa, donde la fosfolipasa comprende: a) la secuencia de aminoácido proporcionada por los aminoácidos 146-153 de la SEC ID NO: 1, los aminoácidos 87-94 de la SEC ID NO: 3, o los aminoácidos 79-86 de la SEC ID NO: 12 o una secuencia idéntica a cualquiera de estas secuencias de aminoácido excepto por la sustitución de un solo aminoácido con otro aminoácido; y b) por lo menos dos residuos cisteína situados en el lado N-terminal de la secuencia proporcionada en a); y c) por lo menos dos residuos cisteína situados en el lado C-terminal de la secuencia proporcionada en a). Reivindicación 25: Una composición detergente que comprende un agente tensoactivo y la fosfolipasa de la reivindicación 15.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200300634 | 2003-04-28 | ||
| DKPA200301163 | 2003-08-14 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR044117A1 true AR044117A1 (es) | 2005-08-24 |
Family
ID=33420359
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP040101441A AR044117A1 (es) | 2003-04-28 | 2004-04-28 | Fosfolipasa y metodo para producirla |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP1620551B1 (es) |
| JP (2) | JP2006524495A (es) |
| AR (1) | AR044117A1 (es) |
| AU (2) | AU2004234472B2 (es) |
| BR (1) | BRPI0409599B1 (es) |
| CA (1) | CA2522954C (es) |
| DK (1) | DK1620551T3 (es) |
| MX (1) | MXPA05011421A (es) |
| NZ (2) | NZ542924A (es) |
| PL (1) | PL1620551T3 (es) |
| RU (2) | RU2363731C2 (es) |
| WO (1) | WO2004097012A2 (es) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
| ATE280221T1 (de) | 1997-04-09 | 2004-11-15 | Danisco | Verwendung von lipase zur verbesserung von teigen und backwaren |
| ES2188190T5 (es) | 1998-07-21 | 2007-11-16 | Danisco A/S | Producto alimentario. |
| CN100591212C (zh) | 2001-05-18 | 2010-02-24 | 丹尼斯科有限公司 | 改善生面团和面包质量的方法 |
| US7955814B2 (en) | 2003-01-17 | 2011-06-07 | Danisco A/S | Method |
| US20050196766A1 (en) | 2003-12-24 | 2005-09-08 | Soe Jorn B. | Proteins |
| MXPA05007653A (es) | 2003-01-17 | 2005-09-30 | Danisco | Metodo. |
| US7718408B2 (en) | 2003-12-24 | 2010-05-18 | Danisco A/S | Method |
| GB0716126D0 (en) | 2007-08-17 | 2007-09-26 | Danisco | Process |
| US7906307B2 (en) | 2003-12-24 | 2011-03-15 | Danisco A/S | Variant lipid acyltransferases and methods of making |
| GB0405637D0 (en) | 2004-03-12 | 2004-04-21 | Danisco | Protein |
| ATE514767T1 (de) | 2004-07-16 | 2011-07-15 | Danisco | Enzymatisches öldegummierungsverfahren |
| EP1900282A1 (en) * | 2006-08-28 | 2008-03-19 | Puratos N.V. | Method of preparing a cake using phospholipase |
| DE102006046719A1 (de) | 2006-10-02 | 2008-04-03 | Ab Enzymes Gmbh | Klonierung, Expression und Verwendung saurer Phospholipasen |
| DE102006046857A1 (de) | 2006-10-02 | 2008-04-03 | Ab Enzymes Gmbh | Klonierung, Expression und Verwendung saurer Lysophospholipasen |
| AU2007344910B2 (en) | 2007-01-25 | 2013-03-28 | Dupont Nutrition Biosciences Aps | Production of a lipid acyltransferase from transformed Bacillus licheniformis cells |
| MX2009008090A (es) | 2007-02-01 | 2009-08-12 | Dsm Ip Assets Bv | Metodo para producir tarta con fosfolipasa a. |
| MX2011012971A (es) * | 2009-06-10 | 2012-11-23 | Puratos Nv | Metodos para preparar panes usando fosfolipasas y composiciones de masa de pan y mezcla de pan que comprenden fosfolipasas. |
| WO2010142695A1 (en) * | 2009-06-10 | 2010-12-16 | Novozymes A/S | Phospholipases and methods of using same |
| EP2343310A1 (en) | 2010-01-08 | 2011-07-13 | Novozymes A/S | Serine hydrolase formulation |
| ES2546630T3 (es) | 2012-01-30 | 2015-09-25 | Dsm Ip Assets B.V. | Alfa-amilasa |
| WO2014073674A1 (ja) | 2012-11-09 | 2014-05-15 | 国立大学法人東北大学 | 糸状菌の高密度培養株を用いた有用物質生産方法 |
| BE1022042B1 (nl) | 2014-09-29 | 2016-02-08 | Puratos Nv | Verbeterde cakebeslagsoorten |
| WO2018114912A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| EP3559221A1 (en) | 2016-12-21 | 2019-10-30 | DSM IP Assets B.V. | Lipolytic enzyme variants |
| WO2018114938A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114941A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| EP4431608A3 (en) | 2017-05-02 | 2025-04-16 | Tohoku University | Mutant filamentous fungus and substance production method in which said mutant filamentous fungus is used |
| MX2020007300A (es) * | 2018-01-15 | 2020-09-25 | Chr Hansen As | Producto de leche fermentada y preparacion del mismo usando fosfolipasa. |
| RU2746817C1 (ru) * | 2020-06-10 | 2021-04-21 | Общество с ограниченной ответственностью "Инновационный центр "Бирюч-новые технологии" | Способ получения штамма-продуцента фосфолипазы А2 Komagataella phaffii (Pichia pastoris) YIB Δleu2_PLA2S |
| WO2024223932A1 (en) | 2023-04-28 | 2024-10-31 | Chr. Hansen A/S | Method of making a food product and uses of a phospholipase |
| CN118685384B (zh) * | 2024-08-07 | 2025-10-17 | 青岛蔚蓝生物集团有限公司 | 溶血磷脂酶突变体 |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1092775A (en) | 1965-07-07 | 1967-11-29 | Knud Aunstrup | Preparation of amyloglucosidase |
| GB1525929A (en) | 1974-11-25 | 1978-09-27 | Unilever Ltd | Stabilised emulsions comprising phospholipoprotein |
| NL8302198A (nl) | 1983-06-21 | 1985-01-16 | Unilever Nv | Margarinevetmengsel en werkwijze ter bereiding van een dergelijk vetmengsel. |
| GB8525012D0 (en) | 1985-10-10 | 1985-11-13 | Cpc International Inc | Carbohydrate refining process |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| GB8610600D0 (en) | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
| EP0305216B1 (en) | 1987-08-28 | 1995-08-02 | Novo Nordisk A/S | Recombinant Humicola lipase and process for the production of recombinant humicola lipases |
| JP2794574B2 (ja) | 1988-08-11 | 1998-09-10 | 昭和産業株式会社 | リゾレシチンの製造方法 |
| JP2709736B2 (ja) | 1988-08-11 | 1998-02-04 | 昭和産業株式会社 | 油脂の精製方法 |
| GB2247025A (en) | 1990-08-13 | 1992-02-19 | Unilever Plc | Enzymatic dishwashing and rinsing composition |
| JPH0687751B2 (ja) | 1990-09-26 | 1994-11-09 | 辻製油株式会社 | 高濃度のリゾホスファチジルコリンを含むリゾレシチンを採取する方法 |
| DE4115938A1 (de) | 1991-05-16 | 1992-11-19 | Metallgesellschaft Ag | Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen |
| JPH06327468A (ja) * | 1993-03-25 | 1994-11-29 | Asahi Chem Ind Co Ltd | ホスフォリパーゼａ２を生産する実質上純粋な微生物 |
| EP1559776A3 (en) | 1994-06-30 | 2006-01-11 | Novozymes Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system and promoters and terminators for use therein |
| EP0743017B1 (en) | 1995-05-15 | 2004-09-29 | DSM IP Assets B.V. | Application of phospholipases in animal feed |
| WO1997022705A1 (en) | 1995-12-15 | 1997-06-26 | Novo Nordisk A/S | A FUNGUNS WHEREIN THE areA, pepC AND/OR pepE GENES HAVE BEEN INACTIVATED |
| AU719536B2 (en) | 1996-06-28 | 2000-05-11 | Novozymes A/S | A recombinant enzyme with dextranase activitiy |
| CN1262639C (zh) | 1996-09-19 | 2006-07-05 | 诺沃奇梅兹有限公司 | 新的宿主细胞和生产蛋白质的方法 |
| ATE226974T1 (de) * | 1996-12-09 | 2002-11-15 | Novozymes As | Verringerung von phosphorhaltigen substanzen in speiseölen mit hohem anteil an nicht- hydratisierbarem phosphor unter verwendung einer phospholipase, eine phospholipase aus fädenförmigen pilz, welche eine phospholipase a und/oder b-aktivität aufweist |
| AU3719297A (en) | 1997-07-02 | 1999-01-25 | Procter & Gamble Company, The | Dishwashing compositions comprising a phospholipase and an amylase |
| WO1999003962A1 (en) * | 1997-07-18 | 1999-01-28 | The Procter & Gamble Company | Detergent compositions comprising a phospholipase |
| AU754470B2 (en) * | 1998-04-20 | 2002-11-14 | Novozymes A/S | Preparation of dough and baked products |
| WO2000032758A1 (en) | 1998-11-27 | 2000-06-08 | Novozymes A/S | Lipolytic enzyme variants |
| DE69939947D1 (de) | 1998-12-23 | 2009-01-02 | Novozymes As | Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen |
| AU766617B2 (en) * | 1999-03-16 | 2003-10-23 | Novozymes A/S | Process for producing cheese |
| US6399121B1 (en) * | 1999-03-16 | 2002-06-04 | Novozymes A/S | Process for producing cheese |
| US6902887B1 (en) | 1999-03-22 | 2005-06-07 | Novozymes Biotech, Inc. | Methods for monitoring multiple gene expression |
| EP1210414B1 (en) | 1999-08-13 | 2006-05-17 | Novozymes A/S | Alkaline xyloglucanase from malbranchea |
| WO2002000852A2 (en) * | 2000-06-26 | 2002-01-03 | Novozymes A/S | Lipolytic enzyme |
-
2004
- 2004-04-23 CA CA2522954A patent/CA2522954C/en not_active Expired - Lifetime
- 2004-04-23 EP EP04729049.9A patent/EP1620551B1/en not_active Expired - Lifetime
- 2004-04-23 PL PL04729049T patent/PL1620551T3/pl unknown
- 2004-04-23 WO PCT/DK2004/000279 patent/WO2004097012A2/en not_active Ceased
- 2004-04-23 RU RU2005136868/13A patent/RU2363731C2/ru active
- 2004-04-23 MX MXPA05011421A patent/MXPA05011421A/es active IP Right Grant
- 2004-04-23 AU AU2004234472A patent/AU2004234472B2/en not_active Ceased
- 2004-04-23 NZ NZ542924A patent/NZ542924A/en not_active IP Right Cessation
- 2004-04-23 BR BRPI0409599-5A patent/BRPI0409599B1/pt not_active IP Right Cessation
- 2004-04-23 NZ NZ553115A patent/NZ553115A/en not_active IP Right Cessation
- 2004-04-23 JP JP2006504363A patent/JP2006524495A/ja active Pending
- 2004-04-23 DK DK04729049.9T patent/DK1620551T3/da active
- 2004-04-28 AR ARP040101441A patent/AR044117A1/es not_active Application Discontinuation
-
2009
- 2009-01-21 AU AU2009200207A patent/AU2009200207B2/en not_active Ceased
- 2009-03-30 RU RU2009111842/10A patent/RU2009111842A/ru not_active Application Discontinuation
-
2010
- 2010-04-19 JP JP2010095997A patent/JP5189130B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1620551B1 (en) | 2013-09-18 |
| NZ553115A (en) | 2008-04-30 |
| NZ542924A (en) | 2008-01-31 |
| WO2004097012A3 (en) | 2005-02-03 |
| RU2009111842A (ru) | 2010-10-10 |
| CA2522954C (en) | 2014-06-17 |
| RU2363731C2 (ru) | 2009-08-10 |
| MXPA05011421A (es) | 2006-05-31 |
| PL1620551T3 (pl) | 2014-03-31 |
| AU2009200207A1 (en) | 2009-02-12 |
| BRPI0409599B1 (pt) | 2014-09-16 |
| BRPI0409599A (pt) | 2006-05-02 |
| JP5189130B2 (ja) | 2013-04-24 |
| JP2010172343A (ja) | 2010-08-12 |
| DK1620551T3 (da) | 2014-01-13 |
| WO2004097012A2 (en) | 2004-11-11 |
| CA2522954A1 (en) | 2004-11-11 |
| JP2006524495A (ja) | 2006-11-02 |
| AU2004234472B2 (en) | 2009-01-08 |
| AU2004234472A1 (en) | 2004-11-11 |
| EP1620551A2 (en) | 2006-02-01 |
| RU2005136868A (ru) | 2006-05-27 |
| AU2009200207B2 (en) | 2011-09-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FC | Refusal |