AR059316A2 - Planta y semilla transformada con antralinato sintesa resistente a la inhibicion por l-triptofano - Google Patents
Planta y semilla transformada con antralinato sintesa resistente a la inhibicion por l-triptofanoInfo
- Publication number
- AR059316A2 AR059316A2 ARP070100466A ARP070100466A AR059316A2 AR 059316 A2 AR059316 A2 AR 059316A2 AR P070100466 A ARP070100466 A AR P070100466A AR P070100466 A ARP070100466 A AR P070100466A AR 059316 A2 AR059316 A2 AR 059316A2
- Authority
- AR
- Argentina
- Prior art keywords
- tryptophan
- plant
- transformed
- anthranilate
- plant cells
- Prior art date
Links
- 230000005764 inhibitory process Effects 0.000 title abstract 2
- 241000196324 Embryophyta Species 0.000 abstract 11
- 108010037870 Anthranilate Synthase Proteins 0.000 abstract 8
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 abstract 8
- 229960004799 tryptophan Drugs 0.000 abstract 6
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 abstract 4
- 150000001413 amino acids Chemical class 0.000 abstract 4
- 241001057636 Dracaena deremensis Species 0.000 abstract 2
- 240000008042 Zea mays Species 0.000 abstract 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 235000005822 corn Nutrition 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
- C12N15/8254—Tryptophan or lysine
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La presente proporciona un método para impartir tolerancia a un aminoácido análogo al triptofano en células de plantas, por medio de introducir y expresar un segmento aislado de ADN que codifica una antranilato sintetasa en las células de la planta. Se proveen asimismo plantas transgénicas transformadas con un segmento de ADN aislado que codifica una antranilato sintetasa, así como semillas y progenie derivada de esas plantas. La presente proporciona asimismo una secuencia de ADNc de una subunidad alfa y una subunidad beta de una antranilato sintetasa de maíz. Reivindicacion 1: Una planta transformada, caracterizada porque ha sido regenerada a partir de las células de planta transformadas obtenidas mediante el método de impartir tolerancia frente a un aminoácido análogo del triptofano a una célula de planta de maíz que comprende los pasos de: a) introducir un casete de expresion que comprende un primer segmento de ADN que codifica una antranilato sintasa exogena ligada operablemente a un promotor distinto de un promotor antranilato sintasa nativa, funcional en una célula de planta de maíz, en células de una planta susceptible, a efectos de producir células de planta transformadas, en donde la antranilato sintasa es sustancialmente resistente a la inhibicion por L-triptofano libre o por un aminoácido análogo del triptofano; y b) expresar la antranilato sintasa codificada por el primer segmento de ADN en las células de planta de modo tal de producir una cantidad de dicha antranilato sintasa efectiva para lograr que las células de planta transformadas sean sustancialmente tolerantes a una cantidad de L-triptofano libre o de un aminoácido análogo del triptofano que inhibe el crecimiento de la células no transformadas de la planta susceptible.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/604,789 US6118047A (en) | 1993-08-25 | 1996-01-19 | Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR059316A2 true AR059316A2 (es) | 2008-03-26 |
Family
ID=24421060
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP970100213A AR005664A1 (es) | 1996-01-19 | 1997-01-20 | Un metodo para impartir tolerancia frente a un aminoacido analogo al triptofano a una celula de planta de maiz, un metodo para seleccionar celulas de planta de maiz transformadas y un metodo para alterar el contenido de triptofano en una planta de maiz |
| ARP980101552A AR011462A2 (es) | 1996-01-19 | 1998-04-06 | UN METODO PARA IMPARTIR TOLERANCIA FRENTE A UN AMINOÁCIDO ANÁLOGO DEL TRIPTOFANO, A UNA CÉLULA DE PLANTA DICOTILEDoNEA, UN MÉTODO PARA ALTERAR EL CONTENIDO DE TRIPTOFANO EN UNA PLANTA DICOTILEDoNEA Y UN MÉTODO PARA SELECCIONAR CÉLULAS DE PLANTAS DICOTILEDoNEAS. |
| ARP070100466A AR059316A2 (es) | 1996-01-19 | 2007-02-05 | Planta y semilla transformada con antralinato sintesa resistente a la inhibicion por l-triptofano |
| ARP070100465A AR059315A2 (es) | 1996-01-19 | 2007-02-05 | Planta y semilla transformada |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP970100213A AR005664A1 (es) | 1996-01-19 | 1997-01-20 | Un metodo para impartir tolerancia frente a un aminoacido analogo al triptofano a una celula de planta de maiz, un metodo para seleccionar celulas de planta de maiz transformadas y un metodo para alterar el contenido de triptofano en una planta de maiz |
| ARP980101552A AR011462A2 (es) | 1996-01-19 | 1998-04-06 | UN METODO PARA IMPARTIR TOLERANCIA FRENTE A UN AMINOÁCIDO ANÁLOGO DEL TRIPTOFANO, A UNA CÉLULA DE PLANTA DICOTILEDoNEA, UN MÉTODO PARA ALTERAR EL CONTENIDO DE TRIPTOFANO EN UNA PLANTA DICOTILEDoNEA Y UN MÉTODO PARA SELECCIONAR CÉLULAS DE PLANTAS DICOTILEDoNEAS. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP070100465A AR059315A2 (es) | 1996-01-19 | 2007-02-05 | Planta y semilla transformada |
Country Status (11)
| Country | Link |
|---|---|
| US (5) | US6118047A (es) |
| EP (1) | EP0879292A1 (es) |
| JP (1) | JP2000503541A (es) |
| AR (4) | AR005664A1 (es) |
| AU (1) | AU724987B2 (es) |
| BR (1) | BR9707055A (es) |
| CA (1) | CA2243268A1 (es) |
| HU (1) | HUP9900387A3 (es) |
| IL (1) | IL125070A0 (es) |
| WO (1) | WO1997026366A1 (es) |
| ZA (1) | ZA97399B (es) |
Families Citing this family (156)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7705215B1 (en) | 1990-04-17 | 2010-04-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US6946587B1 (en) * | 1990-01-22 | 2005-09-20 | Dekalb Genetics Corporation | Method for preparing fertile transgenic corn plants |
| US6118047A (en) * | 1993-08-25 | 2000-09-12 | Dekalb Genetic Corporation | Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction |
| US6563025B1 (en) * | 1996-07-26 | 2003-05-13 | Board Of Trustees Of The University Of Illinois | Nucleotide sequences encoding anthranilate synthase |
| US5965727A (en) * | 1996-07-26 | 1999-10-12 | Board Of Trustees Of The University Of Illinois | For selectable markers and promoters for plant tissue culture transformation |
| EP1026249B1 (en) * | 1997-08-29 | 2008-12-10 | Hokko Chemical Industry Co. Ltd. | GENE ENCODING alpha-SUBUNIT OF RICE ANTHRANILATE SYNTHASE AND DNA RELATING THERETO |
| US7053282B1 (en) * | 1998-02-09 | 2006-05-30 | Pioneer Hi-Bred International, Inc. | Alteration of amino acid compositions in seeds |
| WO1999049058A2 (en) * | 1998-03-26 | 1999-09-30 | E.I. Du Pont De Nemours And Company | Tryptophan biosynthetic enzymes |
| EP1098963A2 (en) * | 1998-07-21 | 2001-05-16 | E.I. Du Pont De Nemours And Company | Chorismate synthase from plants |
| US6653531B1 (en) | 1998-07-21 | 2003-11-25 | E. I. Du Pont De Nemours And Company | Chorismate synthase from plants |
| US6911331B2 (en) | 1998-07-21 | 2005-06-28 | E. I. Du Pont De Nemours And Company | Chorismate biosynthesis enzymes |
| US6429357B1 (en) | 1999-05-14 | 2002-08-06 | Dekalb Genetics Corp. | Rice actin 2 promoter and intron and methods for use thereof |
| US7485715B2 (en) * | 1999-06-18 | 2009-02-03 | Ceres, Inc. | Sequence-determined DNA encoding AP2 domain polypeptides |
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| US6580019B1 (en) | 2000-03-09 | 2003-06-17 | Dekalb Genetics Corporation | Non-reciprocal recombination-mediated transgene deletion in transgenic plants |
| US6750379B2 (en) | 2000-03-09 | 2004-06-15 | Dekalb Genetics Corporation | Homologous recombination-mediated transgene alterations in plants |
| US7879540B1 (en) | 2000-08-24 | 2011-02-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| US7091398B2 (en) * | 2001-02-22 | 2006-08-15 | Pioneer Hi-Bred International, Inc. | Isolated sucrose sythase polynucleotides and uses thereof |
| AR035965A1 (es) * | 2001-05-04 | 2004-07-28 | Monsanto Technology Llc | Un adn aislado que codifica para una antranilato sintasa monomerica, el vector ,la semilla y la planta transgenica que lo comprende; el metodo para alterar el contenido de triptofano en dicha planta; el metodo para hacer alimento animal o alimento humano y alimento animal o humano obtenido. |
| US7192758B2 (en) | 2001-07-16 | 2007-03-20 | E. I. Du Pont De Nemours And Company | Polynucleotides encoding phosphoribosylanthranilate isomerase |
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| US7413874B2 (en) | 2002-12-09 | 2008-08-19 | University Of Miami | Nucleic acid encoding fluorescent proteins from aquatic species |
| US7078234B2 (en) | 2002-12-18 | 2006-07-18 | Monsanto Technology Llc | Maize embryo-specific promoter compositions and methods for use thereof |
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| WO2004108903A2 (en) * | 2003-06-06 | 2004-12-16 | Arborgen, Llc | Plant transformation and selection |
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-
1996
- 1996-01-19 US US08/604,789 patent/US6118047A/en not_active Expired - Lifetime
-
1997
- 1997-01-17 EP EP97903052A patent/EP0879292A1/en not_active Withdrawn
- 1997-01-17 JP JP9526289A patent/JP2000503541A/ja active Pending
- 1997-01-17 AU AU17066/97A patent/AU724987B2/en not_active Expired
- 1997-01-17 BR BR9707055A patent/BR9707055A/pt not_active Application Discontinuation
- 1997-01-17 WO PCT/US1997/000983 patent/WO1997026366A1/en not_active Ceased
- 1997-01-17 CA CA002243268A patent/CA2243268A1/en not_active Abandoned
- 1997-01-17 IL IL12507097A patent/IL125070A0/xx unknown
- 1997-01-17 HU HU9900387A patent/HUP9900387A3/hu unknown
- 1997-01-17 ZA ZA9700399A patent/ZA97399B/xx unknown
- 1997-01-20 AR ARP970100213A patent/AR005664A1/es active IP Right Grant
-
1998
- 1998-04-06 AR ARP980101552A patent/AR011462A2/es active IP Right Grant
-
1999
- 1999-05-17 US US09/312,721 patent/US6271016B1/en not_active Expired - Lifetime
-
2000
- 2000-12-08 US US09/733,300 patent/US6515201B2/en not_active Expired - Fee Related
-
2003
- 2003-01-29 US US10/353,726 patent/US20030167514A1/en not_active Abandoned
-
2005
- 2005-08-03 US US11/196,176 patent/US7288403B2/en not_active Expired - Fee Related
-
2007
- 2007-02-05 AR ARP070100466A patent/AR059316A2/es not_active Application Discontinuation
- 2007-02-05 AR ARP070100465A patent/AR059315A2/es not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| WO1997026366A1 (en) | 1997-07-24 |
| ZA97399B (en) | 1998-04-06 |
| EP0879292A1 (en) | 1998-11-25 |
| US6271016B1 (en) | 2001-08-07 |
| US20020061570A1 (en) | 2002-05-23 |
| AR011462A2 (es) | 2000-08-16 |
| HUP9900387A2 (hu) | 1999-05-28 |
| HUP9900387A3 (en) | 2001-02-28 |
| JP2000503541A (ja) | 2000-03-28 |
| US20030167514A1 (en) | 2003-09-04 |
| AU724987B2 (en) | 2000-10-05 |
| US6118047A (en) | 2000-09-12 |
| CA2243268A1 (en) | 1997-07-24 |
| AR005664A1 (es) | 1999-07-14 |
| US6515201B2 (en) | 2003-02-04 |
| AR059315A2 (es) | 2008-03-26 |
| US7288403B2 (en) | 2007-10-30 |
| US20060168680A1 (en) | 2006-07-27 |
| IL125070A0 (en) | 1999-01-26 |
| BR9707055A (pt) | 1999-07-20 |
| AU1706697A (en) | 1997-08-11 |
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