AR059316A2 - Planta y semilla transformada con antralinato sintesa resistente a la inhibicion por l-triptofano - Google Patents

Planta y semilla transformada con antralinato sintesa resistente a la inhibicion por l-triptofano

Info

Publication number
AR059316A2
AR059316A2 ARP070100466A ARP070100466A AR059316A2 AR 059316 A2 AR059316 A2 AR 059316A2 AR P070100466 A ARP070100466 A AR P070100466A AR P070100466 A ARP070100466 A AR P070100466A AR 059316 A2 AR059316 A2 AR 059316A2
Authority
AR
Argentina
Prior art keywords
tryptophan
plant
transformed
anthranilate
plant cells
Prior art date
Application number
ARP070100466A
Other languages
English (en)
Inventor
Paul C Anderson
Paul S Chomet
Matthew C Grifford
Alan L Kriz
Original Assignee
Dekalb Genetics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dekalb Genetics Corp filed Critical Dekalb Genetics Corp
Publication of AR059316A2 publication Critical patent/AR059316A2/es

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • CCHEMISTRY; METALLURGY
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254Tryptophan or lysine
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
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  • Biomedical Technology (AREA)
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  • Biochemistry (AREA)
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  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente proporciona un método para impartir tolerancia a un aminoácido análogo al triptofano en células de plantas, por medio de introducir y expresar un segmento aislado de ADN que codifica una antranilato sintetasa en las células de la planta. Se proveen asimismo plantas transgénicas transformadas con un segmento de ADN aislado que codifica una antranilato sintetasa, así como semillas y progenie derivada de esas plantas. La presente proporciona asimismo una secuencia de ADNc de una subunidad alfa y una subunidad beta de una antranilato sintetasa de maíz. Reivindicacion 1: Una planta transformada, caracterizada porque ha sido regenerada a partir de las células de planta transformadas obtenidas mediante el método de impartir tolerancia frente a un aminoácido análogo del triptofano a una célula de planta de maíz que comprende los pasos de: a) introducir un casete de expresion que comprende un primer segmento de ADN que codifica una antranilato sintasa exogena ligada operablemente a un promotor distinto de un promotor antranilato sintasa nativa, funcional en una célula de planta de maíz, en células de una planta susceptible, a efectos de producir células de planta transformadas, en donde la antranilato sintasa es sustancialmente resistente a la inhibicion por L-triptofano libre o por un aminoácido análogo del triptofano; y b) expresar la antranilato sintasa codificada por el primer segmento de ADN en las células de planta de modo tal de producir una cantidad de dicha antranilato sintasa efectiva para lograr que las células de planta transformadas sean sustancialmente tolerantes a una cantidad de L-triptofano libre o de un aminoácido análogo del triptofano que inhibe el crecimiento de la células no transformadas de la planta susceptible.
ARP070100466A 1996-01-19 2007-02-05 Planta y semilla transformada con antralinato sintesa resistente a la inhibicion por l-triptofano AR059316A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/604,789 US6118047A (en) 1993-08-25 1996-01-19 Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction

Publications (1)

Publication Number Publication Date
AR059316A2 true AR059316A2 (es) 2008-03-26

Family

ID=24421060

Family Applications (4)

Application Number Title Priority Date Filing Date
ARP970100213A AR005664A1 (es) 1996-01-19 1997-01-20 Un metodo para impartir tolerancia frente a un aminoacido analogo al triptofano a una celula de planta de maiz, un metodo para seleccionar celulas de planta de maiz transformadas y un metodo para alterar el contenido de triptofano en una planta de maiz
ARP980101552A AR011462A2 (es) 1996-01-19 1998-04-06 UN METODO PARA IMPARTIR TOLERANCIA FRENTE A UN AMINOÁCIDO ANÁLOGO DEL TRIPTOFANO, A UNA CÉLULA DE PLANTA DICOTILEDoNEA, UN MÉTODO PARA ALTERAR EL CONTENIDO DE TRIPTOFANO EN UNA PLANTA DICOTILEDoNEA Y UN MÉTODO PARA SELECCIONAR CÉLULAS DE PLANTAS DICOTILEDoNEAS.
ARP070100466A AR059316A2 (es) 1996-01-19 2007-02-05 Planta y semilla transformada con antralinato sintesa resistente a la inhibicion por l-triptofano
ARP070100465A AR059315A2 (es) 1996-01-19 2007-02-05 Planta y semilla transformada

Family Applications Before (2)

Application Number Title Priority Date Filing Date
ARP970100213A AR005664A1 (es) 1996-01-19 1997-01-20 Un metodo para impartir tolerancia frente a un aminoacido analogo al triptofano a una celula de planta de maiz, un metodo para seleccionar celulas de planta de maiz transformadas y un metodo para alterar el contenido de triptofano en una planta de maiz
ARP980101552A AR011462A2 (es) 1996-01-19 1998-04-06 UN METODO PARA IMPARTIR TOLERANCIA FRENTE A UN AMINOÁCIDO ANÁLOGO DEL TRIPTOFANO, A UNA CÉLULA DE PLANTA DICOTILEDoNEA, UN MÉTODO PARA ALTERAR EL CONTENIDO DE TRIPTOFANO EN UNA PLANTA DICOTILEDoNEA Y UN MÉTODO PARA SELECCIONAR CÉLULAS DE PLANTAS DICOTILEDoNEAS.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP070100465A AR059315A2 (es) 1996-01-19 2007-02-05 Planta y semilla transformada

Country Status (11)

Country Link
US (5) US6118047A (es)
EP (1) EP0879292A1 (es)
JP (1) JP2000503541A (es)
AR (4) AR005664A1 (es)
AU (1) AU724987B2 (es)
BR (1) BR9707055A (es)
CA (1) CA2243268A1 (es)
HU (1) HUP9900387A3 (es)
IL (1) IL125070A0 (es)
WO (1) WO1997026366A1 (es)
ZA (1) ZA97399B (es)

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US20020061570A1 (en) 2002-05-23
AR011462A2 (es) 2000-08-16
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HUP9900387A3 (en) 2001-02-28
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AU724987B2 (en) 2000-10-05
US6118047A (en) 2000-09-12
CA2243268A1 (en) 1997-07-24
AR005664A1 (es) 1999-07-14
US6515201B2 (en) 2003-02-04
AR059315A2 (es) 2008-03-26
US7288403B2 (en) 2007-10-30
US20060168680A1 (en) 2006-07-27
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