AT397514B - Verfahren zur indentifizierung von nukleinsäuren - Google Patents

Verfahren zur indentifizierung von nukleinsäuren Download PDF

Info

Publication number: AT397514B
Authority: AT; Austria
Prior art keywords: affinity; pair; hybridization; components; poly
Prior art date: 1985-01-02

Application number

AT0376785A

Other languages

German (de)

English (en)

Other versions

ATA376785A (de

Inventor

Hans Erik Soederlund

Original Assignee

Orion Yhtymae Oy

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-01-02

Filing date

1985-12-30

Publication date

1994-04-25

1985-12-30 Application filed by Orion Yhtymae Oy filed Critical Orion Yhtymae Oy

1993-09-15 Publication of ATA376785A publication Critical patent/ATA376785A/de

1994-04-25 Application granted granted Critical

1994-04-25 Publication of AT397514B publication Critical patent/AT397514B/de

Links

238000000034 method Methods 0.000 title claims description 28
150000007523 nucleic acids Chemical class 0.000 title claims description 19
108020004707 nucleic acids Proteins 0.000 title claims description 16
102000039446 nucleic acids Human genes 0.000 title claims description 16
239000000523 sample Substances 0.000 claims description 69
238000009396 hybridization Methods 0.000 claims description 35
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 10
239000000203 mixture Substances 0.000 claims description 7
108010090804 Streptavidin Proteins 0.000 claims description 5
229960002685 biotin Drugs 0.000 claims description 5
235000020958 biotin Nutrition 0.000 claims description 5
239000011616 biotin Substances 0.000 claims description 5
239000000126 substance Substances 0.000 claims description 4
108090001008 Avidin Proteins 0.000 claims description 2
229910001385 heavy metal Inorganic materials 0.000 claims description 2
108020004414 DNA Proteins 0.000 description 22
239000000243 solution Substances 0.000 description 21
239000013612 plasmid Substances 0.000 description 17
210000004027 cell Anatomy 0.000 description 13
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
239000012634 fragment Substances 0.000 description 7
238000012360 testing method Methods 0.000 description 7
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 5
239000011780 sodium chloride Substances 0.000 description 5
239000001488 sodium phosphate Substances 0.000 description 5
229910000162 sodium phosphate Inorganic materials 0.000 description 5
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 5
239000007853 buffer solution Substances 0.000 description 4
239000000463 material Substances 0.000 description 4
239000007787 solid Substances 0.000 description 4
241000701161 unidentified adenovirus Species 0.000 description 4
239000000872 buffer Substances 0.000 description 3
230000000694 effects Effects 0.000 description 3
244000005700 microbiome Species 0.000 description 3
108090000623 proteins and genes Proteins 0.000 description 3
241000606153 Chlamydia trachomatis Species 0.000 description 2
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
229920002684 Sepharose Polymers 0.000 description 2
229920004890 Triton X-100 Polymers 0.000 description 2
239000013504 Triton X-100 Substances 0.000 description 2
238000001042 affinity chromatography Methods 0.000 description 2
239000001913 cellulose Substances 0.000 description 2
229920002678 cellulose Polymers 0.000 description 2
229940038705 chlamydia trachomatis Drugs 0.000 description 2
238000005194 fractionation Methods 0.000 description 2
238000011534 incubation Methods 0.000 description 2
230000002285 radioactive effect Effects 0.000 description 2
238000011160 research Methods 0.000 description 2
238000000926 separation method Methods 0.000 description 2
238000005406 washing Methods 0.000 description 2
XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical compound OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 description 1
BRLRJZRHRJEWJY-VCOUNFBDSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-n-[3-[3-(4-azido-2-nitroanilino)propyl-methylamino]propyl]pentanamide Chemical compound C([C@H]1[C@H]2NC(=O)N[C@H]2CS1)CCCC(=O)NCCCN(C)CCCNC1=CC=C(N=[N+]=[N-])C=C1[N+]([O-])=O BRLRJZRHRJEWJY-VCOUNFBDSA-N 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 description 1
229920002307 Dextran Polymers 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
241001131785 Escherichia coli HB101 Species 0.000 description 1
102000008857 Ferritin Human genes 0.000 description 1
108050000784 Ferritin Proteins 0.000 description 1
238000008416 Ferritin Methods 0.000 description 1
102000016943 Muramidase Human genes 0.000 description 1
108010014251 Muramidase Proteins 0.000 description 1
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
239000008118 PEG 6000 Substances 0.000 description 1
229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
239000002202 Polyethylene glycol Substances 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
JGQHTPYIIXRFLR-UHFFFAOYSA-N [2-(9h-fluoren-1-yl)-2-oxoethyl] acetate Chemical class C1C2=CC=CC=C2C2=C1C(C(=O)COC(=O)C)=CC=C2 JGQHTPYIIXRFLR-UHFFFAOYSA-N 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
230000003321 amplification Effects 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
238000009835 boiling Methods 0.000 description 1
244000309466 calf Species 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
229960005091 chloramphenicol Drugs 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical group BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
238000010828 elution Methods 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
238000000605 extraction Methods 0.000 description 1
230000002349 favourable effect Effects 0.000 description 1
239000011521 glass Substances 0.000 description 1
-1 haem compounds Chemical class 0.000 description 1
210000004408 hybridoma Anatomy 0.000 description 1
230000037189 immune system physiology Effects 0.000 description 1
229910052747 lanthanoid Inorganic materials 0.000 description 1
150000002602 lanthanoids Chemical class 0.000 description 1
239000004816 latex Substances 0.000 description 1
229920000126 latex Polymers 0.000 description 1
239000003446 ligand Substances 0.000 description 1
229960000274 lysozyme Drugs 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
238000007899 nucleic acid hybridization Methods 0.000 description 1
239000002773 nucleotide Substances 0.000 description 1
125000003729 nucleotide group Chemical group 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
239000004033 plastic Substances 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920001223 polyethylene glycol Polymers 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
239000012488 sample solution Substances 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000000758 substrate Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
238000009210 therapy by ultrasound Methods 0.000 description 1
210000001541 thymus gland Anatomy 0.000 description 1
238000013519 translation Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)
Saccharide Compounds (AREA)

AT0376785A 1985-01-02 1985-12-30 Verfahren zur indentifizierung von nukleinsäuren AT397514B (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
FI850004A FI72146C (fi)	1985-01-02	1985-01-02	Foerfarande foer identifiering av nukleinsyror.

Publications (2)

Publication Number	Publication Date
ATA376785A ATA376785A (de)	1993-09-15
AT397514B true AT397514B (de)	1994-04-25

Family

ID=8520136

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT0376785A AT397514B (de)	1985-01-02	1985-12-30	Verfahren zur indentifizierung von nukleinsäuren

Country Status (21)

Country	Link
JP (1)	JPH0669400B2 (fr)
AT (1)	AT397514B (fr)
AU (1)	AU561382B2 (fr)
BE (1)	BE903937A (fr)
CA (1)	CA1271705A (fr)
CH (1)	CH666696A5 (fr)
DE (1)	DE3546312A1 (fr)
DK (1)	DK164932C (fr)
FI (1)	FI72146C (fr)
FR (1)	FR2575493B1 (fr)
GB (1)	GB2169403B (fr)
HU (1)	HU196453B (fr)
IE (1)	IE58290B1 (fr)
IL (1)	IL77489A (fr)
IT (1)	IT1201514B (fr)
LU (1)	LU86238A1 (fr)
NL (1)	NL189427C (fr)
NO (1)	NO166743C (fr)
RO (1)	RO94651B (fr)
SE (1)	SE463212B (fr)
ZA (1)	ZA859895B (fr)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5288609A (en) *	1984-04-27	1994-02-22	Enzo Diagnostics, Inc.	Capture sandwich hybridization method and composition
US6060237A (en)	1985-02-26	2000-05-09	Biostar, Inc.	Devices and methods for optical detection of nucleic acid hybridization
IL79112A (en) *	1985-06-13	1992-01-15	Abbott Lab	Method for the isolation of a nucleic acid sequence and kit for performing a hybridization assay
US4910300A (en) *	1985-12-11	1990-03-20	Chiron Corporation	Method for making nucleic acid probes
US4868105A (en) *	1985-12-11	1989-09-19	Chiron Corporation	Solution phase nucleic acid sandwich assay
US4882269A (en) *	1985-12-13	1989-11-21	Princeton University	Amplified hybridization assay
FI76119C (fi) *	1986-02-27	1988-09-09	Orion Yhtymae Oy	Kvantitativ bestaemning av nukleinsyramolekyler och reagensfoerpackning som anvaends vid foerfarandet.
AU622104B2 (en) *	1987-03-11	1992-04-02	Sangtec Molecular Diagnostics Ab	Method of assaying of nucleic acids, a reagent combination and kit therefore
FI894132A7 (fi) *	1987-06-26	1989-09-01	Du Pont	Kontaminoivien sekvenssien affiniteettipoisto kloonatusta yhdistelmänu kleiinihaposta kaappaushelmiä käyttämällä
KR960002234B1 (ko) *	1987-07-31	1996-02-13	젠-프로우브 인코오퍼레이티드	바람직하지 못한 교차반응을 제거하기 위한 올리고뉴클레오티드를 사용하는 폴리뉴클레오티드에 대한 측정방법
EP0305145A3 (fr) *	1987-08-24	1990-05-02	Ortho Diagnostic Systems Inc.	Méthodes et sondes pour détecter des acides nucléiques
EP0304845A3 (fr) *	1987-08-28	1991-03-06	Profile Diagnostic Sciences Inc.	Procédé et composition pour déterminer l'expression des gènes
CA1325761C (fr) *	1987-12-25	1994-01-04	Takanori Oka	Methode pour la detection d'un acide nucleique prevu dans un echantillon
DE3800644A1 (de) *	1988-01-12	1989-07-20	Boehringer Mannheim Gmbh	Verfahren zum hochspezifischen nachweis von nukleinsaeuren in loesung
US5104791A (en) *	1988-02-09	1992-04-14	E. I. Du Pont De Nemours And Company	Particle counting nucleic acid hybridization assays
EP0436547B1 (fr) *	1988-05-10	1994-11-30	E.I. Du Pont De Nemours And Company	Procede de detection rapide d'acide nucleique
US5185243A (en) *	1988-08-25	1993-02-09	Syntex (U.S.A.) Inc.	Method for detection of specific nucleic acid sequences
AU629845B2 (en) *	1988-08-30	1992-10-15	Abbott Laboratories	Detection and amplification of target nucleic acid sequences
US5858652A (en) *	1988-08-30	1999-01-12	Abbott Laboratories	Detection and amplification of target nucleic acid sequences
GB2225112A (en) *	1988-11-22	1990-05-23	Ici Plc	Hybridisation probes
US5082935A (en) *	1988-12-15	1992-01-21	Amoco Corporation	Diagnostic reagents made by attaching cytidine containing nucleic acid probes to amino functionalized solid supports by bisulfite mediated transamination
JP3015462B2 (ja) *	1989-02-06	2000-03-06	ジーン‐トラック・システムス	リステリア検出のためのプローブ類及び方法
AU5269590A (en)	1989-03-10	1990-10-09	Gene-Trak Systems	Immobilized oligonucleotide probes and uses therefor
EP0481999B1 (fr) *	1989-03-10	1997-07-23	VYSIS, Inc.	Empechement d'interferences avec des combinaisons de capture d'affinites
WO1991000926A1 (fr) *	1989-07-11	1991-01-24	Microprobe Corporation	Quantification de bacteries au moyen d'un dosage d'hybridation d'acide nucleique
US5334501A (en) *	1989-07-11	1994-08-02	Microprobe Corporation	Quantification of bacteria using a nucleic acid hybridization assay
GB8924989D0 (en) *	1989-11-06	1989-12-28	Scotgen Ltd	Method and device for the detection of antibiotic resistance
US5580970A (en) *	1989-12-01	1996-12-03	Amoco Corporation	Detection of HPV transcripts
AU7429591A (en) *	1990-04-18	1991-10-24	Gene-Trak Systems	Nucleic acid probes for the detection of giardia lamblia
EP0529070A1 (fr) *	1991-02-27	1993-03-03	Amoco Corporation	Procedes d'amelioration de la sensibilite d'essais d'hybridation
WO1993020234A1 (fr) *	1992-03-31	1993-10-14	E.I. Du Pont De Nemours And Company	Dosage rapide, a haute capacite, fonde sur l'acide nucleique
US7713528B1 (en)	1993-02-18	2010-05-11	Enzo Therapeutics, Inc.	Method for in vivo delivery of active compounds using reagent conjugate
WO1997038131A1 (fr) *	1996-04-11	1997-10-16	Valentina Filippovna Kulikova	Procede de detection d'une chaine d'acides nucleiques devant etre analysee
US5853993A (en) *	1996-10-21	1998-12-29	Hewlett-Packard Company	Signal enhancement method and kit
GB0016833D0 (en)	2000-07-07	2000-08-30	Lee Helen	Improved dipstick assays (2)
US7465540B2 (en)	2000-09-21	2008-12-16	Luminex Corporation	Multiple reporter read-out for bioassays
CN1303221C (zh) *	2003-01-27	2007-03-07	英科新创(厦门)科技有限公司	使用亲和放大的集成信号基团的靶核酸检测法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1983002286A1 (fr) *	1981-12-23	1983-07-07	Tchen, Paul	Sonde contenant un acide nucleique modifie et reconnaissable par des anticorps specifiques et utilisation de cette sonde pour detecter et caracteriser une sequence d'adn homologue
US4486539A (en) *	1981-10-16	1984-12-04	Orioon Corporation Ltd.	Detection of microbial nucleic acids by a one-step sandwich hybridization test
EP0139489A2 (fr) *	1983-09-26	1985-05-02	Ortho Diagnostic Systems Inc.	Méthode d'hybridation en sandwich pour la détection d'acides nucléiques

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB8306426D0 (en) *	1983-03-09	1983-04-13	Malcolm A D B	Detecting polynucleotide sequence
CA1228811A (fr) *	1983-05-05	1987-11-03	Robert G. Pergolizzi	Methode d'analyse utilisant des sequences de polynucleotides
ZA849593B (en) *	1983-12-12	1985-07-31	Miles Lab	Hybridization assay employing labeled pairs of hybrid binding reagents
FR2558172B1 (fr) *	1984-01-16	1986-06-13	Inst Nat Sante Rech Med	Sonde contenant un acide nucleique modifie et reconnaissance par des anticorps specifiques et utilisation de cette sonde et de ces anticorps specifiques pour detecter et caracteriser une sequence d'adn homologue
CA1223222A (fr) *	1984-02-22	1987-06-23	Nanibhushan Dattagupta	Sonde moleculaire contenant un acide nucleique immobilise
FR2560298B1 (fr) *	1984-02-28	1988-07-15	Mors	Procede et dispositif de reglage avec precision de la chute de pression d'un fluide initialement sous pression elevee, tel qu'un fluide hydraulique par exemple alimentant un groupe hydraulique, et application du procede au dispositif dans un groupe ou une centrale hydraulique, en etant integre ou non, notamment a une table de machine ou a tout dispositif de support d'outillage
NZ211453A (en) *	1984-03-22	1989-01-06	Biotechnology Research Enterpr	Aryl azides, their preparation and use in the detection, localisation and isolation of polynucleotides
US4766062A (en) *	1984-05-07	1988-08-23	Allied Corporation	Displacement polynucleotide assay method and polynucleotide complex reagent therefor

1985
- 1985-01-02 FI FI850004A patent/FI72146C/fi not_active IP Right Cessation
- 1985-12-17 CH CH5378/85A patent/CH666696A5/de not_active IP Right Cessation
- 1985-12-20 GB GB08531414A patent/GB2169403B/en not_active Expired
- 1985-12-24 BE BE0/216059A patent/BE903937A/fr not_active IP Right Cessation
- 1985-12-27 JP JP60299797A patent/JPH0669400B2/ja not_active Expired - Lifetime
- 1985-12-27 NO NO855308A patent/NO166743C/no not_active IP Right Cessation
- 1985-12-29 HU HU855030A patent/HU196453B/hu unknown
- 1985-12-30 ZA ZA859895A patent/ZA859895B/xx unknown
- 1985-12-30 DE DE19853546312 patent/DE3546312A1/de active Granted
- 1985-12-30 IT IT23408/85A patent/IT1201514B/it active
- 1985-12-30 LU LU86238A patent/LU86238A1/fr unknown
- 1985-12-30 RO RO121637A patent/RO94651B/ro unknown
- 1985-12-30 FR FR858519394A patent/FR2575493B1/fr not_active Expired - Lifetime
- 1985-12-30 AT AT0376785A patent/AT397514B/de not_active IP Right Cessation
- 1985-12-31 NL NLAANVRAGE8503597,A patent/NL189427C/xx not_active IP Right Cessation
- 1985-12-31 IL IL77489A patent/IL77489A/xx not_active IP Right Cessation
- 1985-12-31 CA CA000498834A patent/CA1271705A/fr not_active Expired - Lifetime
- 1985-12-31 AU AU51748/85A patent/AU561382B2/en not_active Expired
- 1985-12-31 IE IE333385A patent/IE58290B1/en not_active IP Right Cessation
1986
- 1986-01-02 SE SE8600011A patent/SE463212B/sv not_active IP Right Cessation
- 1986-01-02 DK DK000386A patent/DK164932C/da not_active IP Right Cessation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4486539A (en) *	1981-10-16	1984-12-04	Orioon Corporation Ltd.	Detection of microbial nucleic acids by a one-step sandwich hybridization test
WO1983002286A1 (fr) *	1981-12-23	1983-07-07	Tchen, Paul	Sonde contenant un acide nucleique modifie et reconnaissable par des anticorps specifiques et utilisation de cette sonde pour detecter et caracteriser une sequence d'adn homologue
EP0139489A2 (fr) *	1983-09-26	1985-05-02	Ortho Diagnostic Systems Inc.	Méthode d'hybridation en sandwich pour la détection d'acides nucléiques

Also Published As

Publication number	Publication date
SE463212B (sv)	1990-10-22
NL189427C (nl)	1993-04-01
GB2169403B (en)	1988-06-08
HUT40166A (en)	1986-11-28
CH666696A5 (de)	1988-08-15
FI850004L (fi)	1986-07-03
IL77489A (en)	1991-01-31
IE853333L (en)	1986-07-02
FI850004A0 (fi)	1985-01-02
NO166743B (no)	1991-05-21
FR2575493B1 (fr)	1990-01-26
DE3546312C2 (fr)	1992-08-06
LU86238A1 (fr)	1986-04-14
NO855308L (no)	1986-07-03
NO166743C (no)	1991-08-28
AU561382B2 (en)	1987-05-07
JPS61185200A (ja)	1986-08-18
DK164932C (da)	1993-01-25
GB8531414D0 (en)	1986-02-05
RO94651B (ro)	1988-07-01
DE3546312A1 (de)	1986-07-10
DK386A (da)	1986-07-03
NL8503597A (nl)	1986-08-01
ZA859895B (en)	1986-08-27
SE8600011L (sv)	1986-07-03
IE58290B1 (en)	1993-08-25
CA1271705A (fr)	1990-07-17
NL189427B (nl)	1992-11-02
IT8523408A0 (it)	1985-12-30
ATA376785A (de)	1993-09-15
FR2575493A1 (fr)	1986-07-04
FI72146B (fi)	1986-12-31
HU196453B (en)	1988-11-28
JPH0669400B2 (ja)	1994-09-07
SE8600011D0 (sv)	1986-01-02
BE903937A (fr)	1986-04-16
AU5174885A (en)	1986-07-17
FI72146C (fi)	1987-04-13
DK164932B (da)	1992-09-07
IT1201514B (it)	1989-02-02
GB2169403A (en)	1986-07-09
DK386D0 (da)	1986-01-02
RO94651A (fr)	1988-06-30

Legal Events

Date	Code	Title
1989-02-15	ELJ	Ceased due to non-payment of the annual fee
1996-12-15	EIH	Change in the person of patent owner
2003-09-15	EIH	Change in the person of patent owner
2006-09-15	ELA	Expired due to lapse of time

Publication	Publication Date	Title
AT397514B (de)	1994-04-25	Verfahren zur indentifizierung von nukleinsäuren
DE3586660T2 (de)	1993-01-07	Hybridisierungstest fuer nukleinsaeure.
DE69216640T2 (de)	1997-06-19	Verfahren und Gerät zum Nachweis von Nukleinsäurensequenzen.
DE69029726T2 (de)	1997-09-04	Festphasendiagnose von medizinischen konditionen
AT395434B (de)	1992-12-28	Verbessertes verfahren zum nachweis von nucleinsaeuren und reagenzienkombination und besteck zu seiner durchfuehrung
DE69122457T2 (de)	1997-03-20	Verfahren zur Herstellung eines Polynukleotides zur Verwendung bei Einzelprimeramplifikation
DE68927373T2 (de)	1997-03-20	Verfahren und mittel zum nachweis von nukleinsäuresequenzen
DE69836587T2 (de)	2007-10-11	Nukleinsäuresammlung
DE2915082C2 (fr)	1989-07-13
DE69419461T2 (de)	2000-04-13	Vorrichtung zum Auffangen von zielmolekülen und diese Vorrichtung benutzendes Auffangverfahren
DE3855709T2 (de)	1997-04-17	Hybridisierungsverfahren und Reagenzsatz dafür
DE3211311A1 (de)	1982-10-07	Verfahren zum nachweis einer verdaechtigen viralen desoxyribonukleinsaeure in einer azellulaeren biologischen fluessigkeit
DE68923603T2 (de)	1996-01-04	Nukleotid Sonden.
DE3850468T2 (de)	1995-01-19	System zum Detektierung von ONS.
CH621147A5 (en)	1981-01-15	Process for obtaining a cell culture which produces a desired polypeptide by genetic modification of microorganisms
DE69012440T2 (de)	1995-02-09	Verfahren zur Bestimmung von Nukleinsäuren.
Dahlén et al.	1987	Sensitive detection of genes by sandwich hybridization and time-resolved fluorometry
EP0122614B1 (fr)	1993-07-14	Trousse de réactifs pour le marquage chimique terminal de l'ADN
DE68923608T2 (de)	1996-01-04	Verfahren zur Vorbereitung von Nukleotid-Sonden unter Verwendung eines hybridizierbaren Komplementärelements.
DE69030804T2 (de)	1997-11-06	Reagenzien zur verbesserung von hybridisierung
DE102009032502A1 (de)	2011-01-20	Detektion von Antigenen
DE68919360T2 (de)	1995-05-18	Verfahren zur reinigung hochreiner heparinase in grosser menge.
EP0729580B1 (fr)	2000-02-02	Procede d'identification d'anticorps specifiques contre l'hdm-2
EP0314904A1 (fr)	1989-05-10	Méthode et réactif pour exécuter des réactions d'hybridations
DE4339533C2 (de)	1997-07-03	Nachweisverfahren für hdm-2 spezifische Antikörper