CH666696A5 - Verfahren zur identifizierung von nucleinsaeuren. - Google Patents
Verfahren zur identifizierung von nucleinsaeuren. Download PDFInfo
- Publication number
- CH666696A5 CH666696A5 CH5378/85A CH537885A CH666696A5 CH 666696 A5 CH666696 A5 CH 666696A5 CH 5378/85 A CH5378/85 A CH 5378/85A CH 537885 A CH537885 A CH 537885A CH 666696 A5 CH666696 A5 CH 666696A5
- Authority
- CH
- Switzerland
- Prior art keywords
- affinity
- pair
- hybridization
- poly
- components
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 24
- 150000007523 nucleic acids Chemical class 0.000 title claims description 20
- 108020004707 nucleic acids Proteins 0.000 title claims description 16
- 102000039446 nucleic acids Human genes 0.000 title claims description 16
- 239000000523 sample Substances 0.000 claims description 63
- 238000009396 hybridization Methods 0.000 claims description 32
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 10
- 108010090804 Streptavidin Proteins 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 229960002685 biotin Drugs 0.000 claims description 5
- 235000020958 biotin Nutrition 0.000 claims description 5
- 239000011616 biotin Substances 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 108090001008 Avidin Proteins 0.000 claims description 2
- 108020004414 DNA Proteins 0.000 description 23
- 239000000243 solution Substances 0.000 description 21
- 239000013612 plasmid Substances 0.000 description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 239000012634 fragment Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 5
- 239000001488 sodium phosphate Substances 0.000 description 5
- 229910000162 sodium phosphate Inorganic materials 0.000 description 5
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 5
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 229910052708 sodium Inorganic materials 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 241000701161 unidentified adenovirus Species 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000606153 Chlamydia trachomatis Species 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 229940038705 chlamydia trachomatis Drugs 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- -1 haem compounds Chemical class 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- BRLRJZRHRJEWJY-VCOUNFBDSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-n-[3-[3-(4-azido-2-nitroanilino)propyl-methylamino]propyl]pentanamide Chemical compound C([C@H]1[C@H]2NC(=O)N[C@H]2CS1)CCCC(=O)NCCCN(C)CCCNC1=CC=C(N=[N+]=[N-])C=C1[N+]([O-])=O BRLRJZRHRJEWJY-VCOUNFBDSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241001131785 Escherichia coli HB101 Species 0.000 description 1
- 102000008857 Ferritin Human genes 0.000 description 1
- 108050000784 Ferritin Proteins 0.000 description 1
- 238000008416 Ferritin Methods 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 239000008118 PEG 6000 Substances 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical group BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 230000037189 immune system physiology Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 229910052747 lanthanoid Inorganic materials 0.000 description 1
- 150000002602 lanthanoids Chemical class 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI850004A FI72146C (fi) | 1985-01-02 | 1985-01-02 | Foerfarande foer identifiering av nukleinsyror. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH666696A5 true CH666696A5 (de) | 1988-08-15 |
Family
ID=8520136
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH5378/85A CH666696A5 (de) | 1985-01-02 | 1985-12-17 | Verfahren zur identifizierung von nucleinsaeuren. |
Country Status (21)
| Country | Link |
|---|---|
| JP (1) | JPH0669400B2 (fr) |
| AT (1) | AT397514B (fr) |
| AU (1) | AU561382B2 (fr) |
| BE (1) | BE903937A (fr) |
| CA (1) | CA1271705A (fr) |
| CH (1) | CH666696A5 (fr) |
| DE (1) | DE3546312A1 (fr) |
| DK (1) | DK164932C (fr) |
| FI (1) | FI72146C (fr) |
| FR (1) | FR2575493B1 (fr) |
| GB (1) | GB2169403B (fr) |
| HU (1) | HU196453B (fr) |
| IE (1) | IE58290B1 (fr) |
| IL (1) | IL77489A (fr) |
| IT (1) | IT1201514B (fr) |
| LU (1) | LU86238A1 (fr) |
| NL (1) | NL189427C (fr) |
| NO (1) | NO166743C (fr) |
| RO (1) | RO94651B (fr) |
| SE (1) | SE463212B (fr) |
| ZA (1) | ZA859895B (fr) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5288609A (en) * | 1984-04-27 | 1994-02-22 | Enzo Diagnostics, Inc. | Capture sandwich hybridization method and composition |
| US6060237A (en) | 1985-02-26 | 2000-05-09 | Biostar, Inc. | Devices and methods for optical detection of nucleic acid hybridization |
| IL79112A (en) * | 1985-06-13 | 1992-01-15 | Abbott Lab | Method for the isolation of a nucleic acid sequence and kit for performing a hybridization assay |
| US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
| US4910300A (en) * | 1985-12-11 | 1990-03-20 | Chiron Corporation | Method for making nucleic acid probes |
| US4882269A (en) * | 1985-12-13 | 1989-11-21 | Princeton University | Amplified hybridization assay |
| FI76119C (fi) * | 1986-02-27 | 1988-09-09 | Orion Yhtymae Oy | Kvantitativ bestaemning av nukleinsyramolekyler och reagensfoerpackning som anvaends vid foerfarandet. |
| AU622104B2 (en) * | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
| EP0365595A4 (fr) * | 1987-06-26 | 1990-06-05 | Du Pont | Extraction par affinite de sequences contaminantes a partir d'acides nucleiques clones recombinants au moyen de perles de capture. |
| DE3851810T2 (de) * | 1987-07-31 | 1995-02-09 | Gen Probe Inc | Polynukleotidentest unter Benutzung von Oligonukleotiden zur Eliminierung von unerwünschten Kreuzreaktionen. |
| EP0305145A3 (fr) * | 1987-08-24 | 1990-05-02 | Ortho Diagnostic Systems Inc. | Méthodes et sondes pour détecter des acides nucléiques |
| EP0304845A3 (fr) * | 1987-08-28 | 1991-03-06 | Profile Diagnostic Sciences Inc. | Procédé et composition pour déterminer l'expression des gènes |
| CA1325761C (fr) * | 1987-12-25 | 1994-01-04 | Takanori Oka | Methode pour la detection d'un acide nucleique prevu dans un echantillon |
| DE3800644A1 (de) * | 1988-01-12 | 1989-07-20 | Boehringer Mannheim Gmbh | Verfahren zum hochspezifischen nachweis von nukleinsaeuren in loesung |
| US5104791A (en) * | 1988-02-09 | 1992-04-14 | E. I. Du Pont De Nemours And Company | Particle counting nucleic acid hybridization assays |
| EP0436547B1 (fr) * | 1988-05-10 | 1994-11-30 | E.I. Du Pont De Nemours And Company | Procede de detection rapide d'acide nucleique |
| US5185243A (en) * | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
| AU629845B2 (en) * | 1988-08-30 | 1992-10-15 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
| US5858652A (en) * | 1988-08-30 | 1999-01-12 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
| GB2225112A (en) * | 1988-11-22 | 1990-05-23 | Ici Plc | Hybridisation probes |
| US5082935A (en) * | 1988-12-15 | 1992-01-21 | Amoco Corporation | Diagnostic reagents made by attaching cytidine containing nucleic acid probes to amino functionalized solid supports by bisulfite mediated transamination |
| EP0418346A1 (fr) * | 1989-02-06 | 1991-03-27 | Gene-Trak Systems | Sondes et procedes ameliores pour la detection de listeria |
| WO1990010717A1 (fr) * | 1989-03-10 | 1990-09-20 | Gene-Trak Systems | Empechement d'interferences avec des combinaisons de capture d'affinites |
| ATE118824T1 (de) | 1989-03-10 | 1995-03-15 | Amoco Corp | Immobilisierte oligonukleotidsonden und ihre verwendungen. |
| AU6075490A (en) * | 1989-07-11 | 1991-02-06 | Microprobe Corporation | Quantification of bacteria using a nucleic acid hybridization assay |
| US5334501A (en) * | 1989-07-11 | 1994-08-02 | Microprobe Corporation | Quantification of bacteria using a nucleic acid hybridization assay |
| GB8924989D0 (en) * | 1989-11-06 | 1989-12-28 | Scotgen Ltd | Method and device for the detection of antibiotic resistance |
| US5580970A (en) * | 1989-12-01 | 1996-12-03 | Amoco Corporation | Detection of HPV transcripts |
| AU7429591A (en) * | 1990-04-18 | 1991-10-24 | Gene-Trak Systems | Nucleic acid probes for the detection of giardia lamblia |
| EP0529070A1 (fr) * | 1991-02-27 | 1993-03-03 | Amoco Corporation | Procedes d'amelioration de la sensibilite d'essais d'hybridation |
| WO1993020234A1 (fr) * | 1992-03-31 | 1993-10-14 | E.I. Du Pont De Nemours And Company | Dosage rapide, a haute capacite, fonde sur l'acide nucleique |
| US7713528B1 (en) | 1993-02-18 | 2010-05-11 | Enzo Therapeutics, Inc. | Method for in vivo delivery of active compounds using reagent conjugate |
| RU2146707C1 (ru) * | 1996-04-11 | 2000-03-20 | Куликова Валентина Филипповна | Способ выявления анализируемой последовательности нуклеиновых кислот |
| US5853993A (en) * | 1996-10-21 | 1998-12-29 | Hewlett-Packard Company | Signal enhancement method and kit |
| GB0016833D0 (en) | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (2) |
| US7465540B2 (en) | 2000-09-21 | 2008-12-16 | Luminex Corporation | Multiple reporter read-out for bioassays |
| CN1303221C (zh) * | 2003-01-27 | 2007-03-07 | 英科新创(厦门)科技有限公司 | 使用亲和放大的集成信号基团的靶核酸检测法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI63596C (fi) * | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
| FR2518755B1 (fr) * | 1981-12-23 | 1986-04-11 | Pasteur Institut | Sonde contenant un acide nucleique modifie et reconnaissable par des anticorps specifiques et utilisation de cette sonde pour detecter et caracteriser une sequence d'adn homologue |
| GB8306426D0 (en) * | 1983-03-09 | 1983-04-13 | Malcolm A D B | Detecting polynucleotide sequence |
| CA1228811A (fr) * | 1983-05-05 | 1987-11-03 | Robert G. Pergolizzi | Methode d'analyse utilisant des sequences de polynucleotides |
| NO843838L (no) * | 1983-09-26 | 1985-03-27 | Ortho Diagnostic Systems Inc | Fremgangsmaate for detektering av nukleinsyre |
| ZA849595B (en) * | 1983-12-12 | 1985-07-31 | Miles Lab | Hybridization assay employing labeled probe and anti-hybrid |
| FR2558172B1 (fr) * | 1984-01-16 | 1986-06-13 | Inst Nat Sante Rech Med | Sonde contenant un acide nucleique modifie et reconnaissance par des anticorps specifiques et utilisation de cette sonde et de ces anticorps specifiques pour detecter et caracteriser une sequence d'adn homologue |
| CA1223222A (fr) * | 1984-02-22 | 1987-06-23 | Nanibhushan Dattagupta | Sonde moleculaire contenant un acide nucleique immobilise |
| FR2560298B1 (fr) * | 1984-02-28 | 1988-07-15 | Mors | Procede et dispositif de reglage avec precision de la chute de pression d'un fluide initialement sous pression elevee, tel qu'un fluide hydraulique par exemple alimentant un groupe hydraulique, et application du procede au dispositif dans un groupe ou une centrale hydraulique, en etant integre ou non, notamment a une table de machine ou a tout dispositif de support d'outillage |
| NZ211453A (en) * | 1984-03-22 | 1989-01-06 | Biotechnology Research Enterpr | Aryl azides, their preparation and use in the detection, localisation and isolation of polynucleotides |
| US4766062A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
-
1985
- 1985-01-02 FI FI850004A patent/FI72146C/fi not_active IP Right Cessation
- 1985-12-17 CH CH5378/85A patent/CH666696A5/de not_active IP Right Cessation
- 1985-12-20 GB GB08531414A patent/GB2169403B/en not_active Expired
- 1985-12-24 BE BE0/216059A patent/BE903937A/fr not_active IP Right Cessation
- 1985-12-27 JP JP60299797A patent/JPH0669400B2/ja not_active Expired - Lifetime
- 1985-12-27 NO NO855308A patent/NO166743C/no not_active IP Right Cessation
- 1985-12-29 HU HU855030A patent/HU196453B/hu unknown
- 1985-12-30 AT AT0376785A patent/AT397514B/de not_active IP Right Cessation
- 1985-12-30 DE DE19853546312 patent/DE3546312A1/de active Granted
- 1985-12-30 ZA ZA859895A patent/ZA859895B/xx unknown
- 1985-12-30 LU LU86238A patent/LU86238A1/fr unknown
- 1985-12-30 IT IT23408/85A patent/IT1201514B/it active
- 1985-12-30 RO RO121637A patent/RO94651B/ro unknown
- 1985-12-30 FR FR858519394A patent/FR2575493B1/fr not_active Expired - Lifetime
- 1985-12-31 IL IL77489A patent/IL77489A/xx not_active IP Right Cessation
- 1985-12-31 AU AU51748/85A patent/AU561382B2/en not_active Expired
- 1985-12-31 CA CA000498834A patent/CA1271705A/fr not_active Expired - Lifetime
- 1985-12-31 NL NLAANVRAGE8503597,A patent/NL189427C/xx not_active IP Right Cessation
- 1985-12-31 IE IE333385A patent/IE58290B1/en not_active IP Right Cessation
-
1986
- 1986-01-02 DK DK000386A patent/DK164932C/da not_active IP Right Cessation
- 1986-01-02 SE SE8600011A patent/SE463212B/sv not_active IP Right Cessation
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AT397514B (de) | Verfahren zur indentifizierung von nukleinsäuren | |
| DE3586659T2 (de) | Hybridizierungstest fuer nukleinsaeure. | |
| DE69029726T2 (de) | Festphasendiagnose von medizinischen konditionen | |
| DE69734263T2 (de) | Verfahren zur Isolierung von Ribonucleinsäuren. | |
| DE69434255T2 (de) | Nukleinsäuresonde zum nachweis von lactobacillus | |
| DE68927373T2 (de) | Verfahren und mittel zum nachweis von nukleinsäuresequenzen | |
| DE3211311A1 (de) | Verfahren zum nachweis einer verdaechtigen viralen desoxyribonukleinsaeure in einer azellulaeren biologischen fluessigkeit | |
| AT395434B (de) | Verbessertes verfahren zum nachweis von nucleinsaeuren und reagenzienkombination und besteck zu seiner durchfuehrung | |
| DE60033825T2 (de) | Detektion von unterschieden in nukleinsäuren durch inhibierung spontaner dna verzweigungsmigration | |
| DE3876108T2 (de) | Diagnostischer test unter verwendung von nucleinsaeure-sonden. | |
| DE69430539T2 (de) | Primers zur genamplifikation von enterotoxin und lecithinase aus clostridium perfringens und deren anwendung zur nachweis und zählung dieser bakterien | |
| DE69009774T2 (de) | Verfahren zum klonieren und reagenziensatz. | |
| CH621147A5 (en) | Process for obtaining a cell culture which produces a desired polypeptide by genetic modification of microorganisms | |
| EP0120376A1 (fr) | Procédé por la réalisation de réactions d'hybridation | |
| DE69012440T2 (de) | Verfahren zur Bestimmung von Nukleinsäuren. | |
| DE3688985T2 (de) | Verfahren und Sonde zum Nachweis von Actinobacillus Actinomycetemcomitans. | |
| Dahlén et al. | Sensitive detection of genes by sandwich hybridization and time-resolved fluorometry | |
| DE69032778T2 (de) | Verfahren zur Untersuchung von durch Mikroorganismen verursachten Lebensmittelvergiftungen und Reagens dafür | |
| NO841725L (no) | Fremgangsmaate og stoffer til bruk ved analyse og paavisning av genetisk materiale | |
| DE69133590T2 (de) | Salmonella Genom Nukleinsäure, ihre Verwendung, besonders bei der in vitro Diagnose der Anwesenheit von Bakterien der Gattung Salmonella in Lebensmitteln | |
| DE68923608T2 (de) | Verfahren zur Vorbereitung von Nukleotid-Sonden unter Verwendung eines hybridizierbaren Komplementärelements. | |
| DE68924601T2 (de) | Reinigung von q-beta-replicase. | |
| DE69130394T2 (de) | Nicht-ätzende zusammensetzung und verfahren zur gewinnung von nukleinsäuren | |
| WO2001068900A2 (fr) | Procede pour la detection specifique de micro-organismes par reaction en chaine de la polymerase | |
| DE68919360T2 (de) | Verfahren zur reinigung hochreiner heparinase in grosser menge. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUE | Assignment |
Owner name: ORION-YHTYMAE OY TRANSFER- SANGTEC MEDICAL AB |
|
| NV | New agent |
Representative=s name: E. BLUM & CO. PATENTANWAELTE |
|
| PUE | Assignment |
Owner name: SANGTEC MEDICAL AB TRANSFER- SANGTEC MOLECULAR DIA |
|
| PL | Patent ceased |