ATE102619T1 - Fluorescierende intrazellulare ca-indikatoren. - Google Patents
Fluorescierende intrazellulare ca-indikatoren.Info
- Publication number
- ATE102619T1 ATE102619T1 AT89304872T AT89304872T ATE102619T1 AT E102619 T1 ATE102619 T1 AT E102619T1 AT 89304872 T AT89304872 T AT 89304872T AT 89304872 T AT89304872 T AT 89304872T AT E102619 T1 ATE102619 T1 AT E102619T1
- Authority
- AT
- Austria
- Prior art keywords
- indicators
- fluorescent intracellular
- hydrogen
- compounds
- intracellular
- Prior art date
Links
- 230000003834 intracellular effect Effects 0.000 title abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 229910052739 hydrogen Inorganic materials 0.000 abstract 2
- 239000001257 hydrogen Substances 0.000 abstract 2
- 125000004435 hydrogen atom Chemical group [H]* 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 1
- -1 acetoxymethyl Chemical group 0.000 abstract 1
- 239000011575 calcium Substances 0.000 abstract 1
- 229910052791 calcium Inorganic materials 0.000 abstract 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 abstract 1
- 229910003002 lithium salt Inorganic materials 0.000 abstract 1
- 159000000002 lithium salts Chemical class 0.000 abstract 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- BITYAPCSNKJESK-UHFFFAOYSA-N potassiosodium Chemical compound [Na].[K] BITYAPCSNKJESK-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
- C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
- C07D403/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
- C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
- C07D405/06—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
- C07D409/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings
- C07D409/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
- C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
- C07D417/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Inorganic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Plural Heterocyclic Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Electroluminescent Light Sources (AREA)
- Luminescent Compositions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/196,654 US4849362A (en) | 1988-05-19 | 1988-05-19 | Fluorescent intracellular calcium indicators |
| EP89304872A EP0342891B1 (de) | 1988-05-19 | 1989-05-15 | Fluorescierende intrazellulare Ca-Indikatoren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE102619T1 true ATE102619T1 (de) | 1994-03-15 |
Family
ID=22726283
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT89304872T ATE102619T1 (de) | 1988-05-19 | 1989-05-15 | Fluorescierende intrazellulare ca-indikatoren. |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4849362A (de) |
| EP (1) | EP0342891B1 (de) |
| JP (2) | JPH0813812B2 (de) |
| AT (1) | ATE102619T1 (de) |
| DE (1) | DE68913586T2 (de) |
| ES (1) | ES2061990T3 (de) |
Families Citing this family (81)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5200345A (en) * | 1989-08-16 | 1993-04-06 | New York University | Methods and apparatus for quantifying tissue damage, determining tissue type, monitoring neural activity, and determining hematocrit |
| US5648270A (en) * | 1995-02-06 | 1997-07-15 | Molecular Probes, Inc. | Methods of sensing with fluorescent conjugates of metal-chelating nitrogen heterocycles |
| US5453517A (en) * | 1992-02-25 | 1995-09-26 | Molecular Probes, Inc. | Reactive derivatives of bapta used to make ion-selective chelators |
| US5501980A (en) * | 1994-05-20 | 1996-03-26 | Molecular Probes, Inc. | Benzazolylcoumarin-based ion indicators |
| US5459276A (en) * | 1994-05-20 | 1995-10-17 | Molecular Probes, Inc. | Benzazolylcoumarin-based ion indicators for heavy metals |
| JP2970967B2 (ja) * | 1991-11-20 | 1999-11-02 | 浜松ホトニクス株式会社 | 蛍光性プローブ試薬を用いた細胞内イオン濃度測定法 |
| US5262330A (en) * | 1992-02-27 | 1993-11-16 | Miles Inc. | Colorimetric methods and reagents for the assay of calcium in a test sample |
| JPH07194393A (ja) * | 1992-08-24 | 1995-08-01 | Hamamatsu Photonics Kk | 生細胞内イオン濃度の３波長測光法のための定数最適化方法 |
| US5409835A (en) * | 1992-12-30 | 1995-04-25 | The University Of Maryland At Baltimore | Long-wavelength fluorescent probe compounds for calcium ions and their use in ratiometrically measuring calcium ion concentrations |
| US5294799A (en) * | 1993-02-01 | 1994-03-15 | Aslund Nils R D | Apparatus for quantitative imaging of multiple fluorophores |
| US5383023A (en) * | 1993-03-01 | 1995-01-17 | Walleczek; Jan | Method and apparatus for performing dual-beam dual-wavelength fluorescence spectrophotometric evaluation of a biological specimen |
| US5773227A (en) * | 1993-06-23 | 1998-06-30 | Molecular Probes, Inc. | Bifunctional chelating polysaccharides |
| AT401823B (de) * | 1993-09-30 | 1996-12-27 | Avl Verbrennungskraft Messtech | Optischer indikator zur bestimmung der aktivität eines ions in einer probe |
| US5516911A (en) * | 1993-12-30 | 1996-05-14 | The United States Of America As Represented By The Department Of Health And Human Services | Fluorescent intracellular calcium indicators |
| US5888829A (en) * | 1994-11-08 | 1999-03-30 | Molecular Probes, Inc. | Photolabile caged ionophores and method of using in a membrane separation process |
| RU2152023C1 (ru) * | 1995-01-30 | 2000-06-27 | Цирельников Николай Иванович | Устройство для прямого определения количественных параметров клеточных структур |
| US5872243A (en) * | 1995-06-30 | 1999-02-16 | Molecular Probes, Inc. | Caged nucleotides |
| IL121844A0 (en) | 1997-09-28 | 1998-02-22 | Dpharm Ltd | Lipophilic diesters of chelating agents |
| US7169922B2 (en) * | 2000-08-04 | 2007-01-30 | Invitrogen Corporation | Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings |
| GB2368843A (en) * | 2000-11-08 | 2002-05-15 | Leuven K U Res & Dev | Non-porphyrin multipurpose contrast agents |
| US6962992B2 (en) | 2000-12-20 | 2005-11-08 | Molecullar Probes, Inc. | Crown ether derivatives |
| US7579463B2 (en) * | 2000-12-20 | 2009-08-25 | Life Technologies Corporation | Crown ether derivatives |
| US8323903B2 (en) | 2001-10-12 | 2012-12-04 | Life Technologies Corporation | Antibody complexes and methods for immunolabeling |
| US20050069962A1 (en) * | 2001-10-12 | 2005-03-31 | Archer Robert M | Antibody complexes and methods for immunolabeling |
| US20040171034A1 (en) * | 2002-05-03 | 2004-09-02 | Brian Agnew | Compositions and methods for detection and isolation of phosphorylated molecules |
| CN1665790A (zh) * | 2002-05-03 | 2005-09-07 | 莫莱丘莱尔探针公司 | 用于检测和分离磷酸化分子的组合物及方法 |
| US7445894B2 (en) * | 2002-05-03 | 2008-11-04 | Molecular Probes, Inc. | Compositions and methods for detection and isolation of phosphorylated molecules |
| US20050233307A1 (en) | 2002-09-12 | 2005-10-20 | Kyle Gee | Site-specific labeling of affinity tags in fusion proteins |
| US8445702B2 (en) * | 2003-05-05 | 2013-05-21 | Life Technologies Corporation | Zinc binding compounds and their method of use |
| US20050250214A1 (en) * | 2004-05-05 | 2005-11-10 | Gee Kyle R | Zinc binding compounds and their method of use |
| US7619059B2 (en) | 2003-07-29 | 2009-11-17 | Life Technologies Corporation | Bimolecular optical probes |
| US7727752B2 (en) * | 2003-07-29 | 2010-06-01 | Life Technologies Corporation | Kinase and phosphatase assays |
| CA2445420A1 (en) | 2003-07-29 | 2005-01-29 | Invitrogen Corporation | Kinase and phosphatase assays |
| US7271265B2 (en) * | 2003-08-11 | 2007-09-18 | Invitrogen Corporation | Cyanine compounds and their application as quenching compounds |
| US20050064485A1 (en) * | 2003-09-12 | 2005-03-24 | Kurt Vogel | Multiplex binding and activity assays |
| US7282339B2 (en) * | 2003-09-17 | 2007-10-16 | Invitrogen Corporation | Competitive immunoassay |
| US20050214807A1 (en) * | 2003-11-19 | 2005-09-29 | Iain Johnson | Environmental sensitive fluorogenic compounds and their application for singlet oxygen and protein detection |
| EP2607431B1 (de) * | 2003-12-05 | 2016-08-24 | Life Technologies Corporation | Cyanin Farbstoffverbindungen |
| US7776529B2 (en) | 2003-12-05 | 2010-08-17 | Life Technologies Corporation | Methine-substituted cyanine dye compounds |
| WO2005071096A2 (en) | 2004-01-21 | 2005-08-04 | Molecular Probes, Inc. | Derivatives of cephalosporin and clavulanic acid for detecting beta-lacamase in a sample |
| WO2006023231A2 (en) * | 2004-07-27 | 2006-03-02 | Molecular Probes, Inc. | Fluorescent metal ion indicators with large stokes shift |
| JP5306811B2 (ja) * | 2005-05-11 | 2013-10-02 | ライフ テクノロジーズ コーポレーション | ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用 |
| US20070134685A1 (en) * | 2005-09-06 | 2007-06-14 | Invitrogen Corporation | Control of chemical modification |
| EP1934607B1 (de) * | 2005-10-13 | 2013-08-28 | Fundação D. Anna Sommer Champalimaud E Dr. Carlos Montez Champalimaud | Multiplex in situ immunochemische analyse |
| US7521577B2 (en) | 2006-01-12 | 2009-04-21 | Molecular Probes, Inc. | Heavy metal binding compounds and their method of use |
| US20070196860A1 (en) * | 2006-01-18 | 2007-08-23 | Invitrogen Corporation | Methods for Measuring Real Time Kinase Activity |
| US8551408B2 (en) | 2006-01-24 | 2013-10-08 | Life Technologies Corporation | Device and methods for quantifying analytes |
| EP1987361A4 (de) * | 2006-01-30 | 2009-03-04 | Invitrogen Corp | Zusammensetzungen und verfahren zur erkennung und quantifizierung toxischer substanzen in krankheitsstadien |
| DK3438131T3 (da) | 2006-02-10 | 2022-04-11 | Life Technologies Corp | Oligosaccharidmodifikation og mærkning af proteiner |
| US8114636B2 (en) | 2006-02-10 | 2012-02-14 | Life Technologies Corporation | Labeling and detection of nucleic acids |
| US8562802B1 (en) | 2006-02-13 | 2013-10-22 | Life Technologies Corporation | Transilluminator base and scanner for imaging fluorescent gels, charging devices and portable electrophoresis systems |
| JP2009536150A (ja) | 2006-03-23 | 2009-10-08 | アブソルバー， アーベー | 診断および治療適用のための異なる型の血液型抗原 |
| AU2007240316A1 (en) | 2006-04-20 | 2007-11-01 | Becton, Dickinson And Company | Thermostable proteins and methods of making and using thereof |
| WO2008076524A2 (en) | 2006-10-27 | 2008-06-26 | Life Technologies Corporation | Fluorogenic ph sensitive dyes and their method of use |
| US8586743B2 (en) * | 2007-01-30 | 2013-11-19 | Life Technologies Corporation | Labeling reagents and methods of their use |
| US9279817B2 (en) | 2007-04-13 | 2016-03-08 | Aat Bioquest, Inc. | Carbofluorescein lactone ion indicators and their applications |
| US20080254498A1 (en) | 2007-04-13 | 2008-10-16 | Abd Bioquest, Inc. | Fluorescent ion indicators and their applications |
| US9097730B2 (en) | 2007-04-13 | 2015-08-04 | Aat Bioquest, Inc. | Fluorescein lactone ion indicators and their applications |
| US8513451B2 (en) * | 2007-05-30 | 2013-08-20 | Hee Chol Kang | Fluorescent phospholipase A2 indicators |
| JP4923298B2 (ja) * | 2007-09-07 | 2012-04-25 | コリア ユニバーシティ インダストリアル アンド アカデミック コラボレイション ファウンデーション | 細胞内カルシウムのリアルタイムモニタリング用二光子プローブ |
| EP2297344B1 (de) * | 2008-05-16 | 2018-03-14 | Life Technologies Corporation | Doppelmarkierungsverfahren zur messung von zellproliferation |
| JP2012519170A (ja) | 2009-02-26 | 2012-08-23 | オーエスアイ・ファーマシューティカルズ，エルエルシー | 生体内の腫瘍細胞のｅｍｔステータスをモニターするためのｉｎｓｉｔｕ法 |
| JP5906190B2 (ja) | 2009-10-17 | 2016-04-20 | アサンテ リサーチ, エルエルシーAsante Research, Llc | レシオメトリックであり、かつ赤色スペクトルで発光する蛍光カルシウム指示薬 |
| US8974651B2 (en) | 2010-04-17 | 2015-03-10 | C.C. Imex | Illuminator for visualization of fluorophores |
| WO2012022734A2 (en) | 2010-08-16 | 2012-02-23 | Medimmune Limited | Anti-icam-1 antibodies and methods of use |
| WO2014066733A2 (en) | 2012-10-25 | 2014-05-01 | Life Technologies Corporation | Methods and compositions for enzyme-mediated site-specific radiolabeling of glycoproteins |
| WO2015050959A1 (en) | 2013-10-01 | 2015-04-09 | Yale University | Anti-kit antibodies and methods of use thereof |
| SI3052192T1 (sl) | 2013-10-02 | 2020-11-30 | Medimmune, Llc | Nevtralizarijoča protitelesa proti-influenci A in njihove uporabe |
| US9636034B2 (en) | 2013-10-23 | 2017-05-02 | Verily Life Sciences Llc | Non-invasive analyte detection system with modulation source |
| US10542918B2 (en) | 2013-10-23 | 2020-01-28 | Verily Life Sciences Llc | Modulation of a response signal to distinguish between analyte and background signals |
| US9835587B2 (en) | 2014-04-01 | 2017-12-05 | C.C. Imex | Electrophoresis running tank assembly |
| MX392761B (es) | 2014-07-15 | 2025-03-21 | Medimmune Llc | Anticuerpos anti-influenza b neutralizantes y usos de los mismos. |
| US10383957B2 (en) | 2015-01-06 | 2019-08-20 | Anthony H. de Haas | Near-infrared fluorescent surgical dye markers |
| US9861710B1 (en) | 2015-01-16 | 2018-01-09 | Verily Life Sciences Llc | Composite particles, methods, and in vivo diagnostic system |
| US10028659B2 (en) | 2015-03-26 | 2018-07-24 | Verily Life Sciences Llc | Aptamer-based sensors, implantable devices and detection system |
| WO2017181176A1 (en) | 2016-04-15 | 2017-10-19 | Lcahn School Of Medicine At Mount Sinai | Tissue profiling using multiplexed immunohistochemical consecutive staining |
| US20210108253A1 (en) | 2017-03-30 | 2021-04-15 | Life Technologies Corporation | Quantification of ngs dna by adapter sequence |
| US9810700B1 (en) | 2017-05-31 | 2017-11-07 | Aat Bioquest, Inc. | Fluorogenic calcium ion indicators and methods of using the same |
| KR20230034333A (ko) | 2020-07-02 | 2023-03-09 | 라이프 테크놀로지스 코포레이션 | 트리뉴클레오티드 캡 유사체, 제조 및 이의 용도 |
| US20250092261A1 (en) | 2021-07-21 | 2025-03-20 | Life Technologies Corporation | Dibenzoxanthene Quenchers, Uses, and Methods of Preparation |
| US12523648B1 (en) * | 2025-02-13 | 2026-01-13 | ION Biosciences | Fluorescent indicators for calcium metal ion detection |
-
1988
- 1988-05-19 US US07/196,654 patent/US4849362A/en not_active Expired - Lifetime
-
1989
- 1989-05-15 EP EP89304872A patent/EP0342891B1/de not_active Expired - Lifetime
- 1989-05-15 DE DE68913586T patent/DE68913586T2/de not_active Expired - Lifetime
- 1989-05-15 ES ES89304872T patent/ES2061990T3/es not_active Expired - Lifetime
- 1989-05-15 AT AT89304872T patent/ATE102619T1/de not_active IP Right Cessation
- 1989-05-19 JP JP1127716A patent/JPH0813812B2/ja not_active Expired - Lifetime
-
1995
- 1995-07-07 JP JP7171874A patent/JP2648293B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH08178850A (ja) | 1996-07-12 |
| DE68913586D1 (de) | 1994-04-14 |
| JPH0222275A (ja) | 1990-01-25 |
| US4849362A (en) | 1989-07-18 |
| JP2648293B2 (ja) | 1997-08-27 |
| EP0342891B1 (de) | 1994-03-09 |
| JPH0813812B2 (ja) | 1996-02-14 |
| DE68913586T2 (de) | 1994-06-23 |
| EP0342891A1 (de) | 1989-11-23 |
| ES2061990T3 (es) | 1994-12-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| EELA | Cancelled due to lapse of time |