ATE49233T1 - Vektoren zur transformation von hefe. - Google Patents

Vektoren zur transformation von hefe.

Info

Publication number: ATE49233T1
Authority: AT; Austria
Prior art keywords: yeast; polypeptides; exogenous; endorphin; secretion
Prior art date: 1983-04-22

Application number

AT84104456T

Other languages

English (en)

Inventor

Grant A Bitter

Original Assignee

Amgen Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-04-22

Filing date

1984-04-19

Publication date

1990-01-15

1984-04-19 Application filed by Amgen Inc filed Critical Amgen Inc

1990-01-15 Application granted granted Critical

1990-01-15 Publication of ATE49233T1 publication Critical patent/ATE49233T1/de

Links

240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 3
230000009466 transformation Effects 0.000 title abstract 3
239000013598 vector Substances 0.000 title abstract 3
108090000765 processed proteins & peptides Proteins 0.000 abstract 8
102000004196 processed proteins & peptides Human genes 0.000 abstract 8
229920001184 polypeptide Polymers 0.000 abstract 7
239000002243 precursor Substances 0.000 abstract 3
230000028327 secretion Effects 0.000 abstract 2
210000005253 yeast cell Anatomy 0.000 abstract 2
102400000748 Beta-endorphin Human genes 0.000 abstract 1
101800005049 Beta-endorphin Proteins 0.000 abstract 1
108020004414 DNA Proteins 0.000 abstract 1
101500024092 Homo sapiens Beta-endorphin Proteins 0.000 abstract 1
101710089743 Mating factor alpha Proteins 0.000 abstract 1
108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
WOPZMFQRCBYPJU-NTXHZHDSSA-N beta-endorphin Chemical compound C([C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CCSC)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)CNC(=O)CNC(=O)[C@@H](N)CC=1C=CC(O)=CC=1)[C@@H](C)O)[C@@H](C)O)C(C)C)[C@@H](C)O)C1=CC=CC=C1 WOPZMFQRCBYPJU-NTXHZHDSSA-N 0.000 abstract 1
230000004071 biological effect Effects 0.000 abstract 1
230000015572 biosynthetic process Effects 0.000 abstract 1
230000003834 intracellular effect Effects 0.000 abstract 1
238000004519 manufacturing process Methods 0.000 abstract 1
239000000463 material Substances 0.000 abstract 1
238000010188 recombinant method Methods 0.000 abstract 1
239000000126 substance Substances 0.000 abstract 1
238000003786 synthesis reaction Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/665—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin
- C07K14/675—Beta-endorphins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Bioinformatics & Cheminformatics (AREA)
Molecular Biology (AREA)
Wood Science & Technology (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Mycology (AREA)
Medicinal Chemistry (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Toxicology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)

AT84104456T 1983-04-22 1984-04-19 Vektoren zur transformation von hefe. ATE49233T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US48775383A	1983-04-22	1983-04-22
EP84104456A EP0123294B1 (de)	1983-04-22	1984-04-19	Vektoren zur Transformation von Hefe

Publications (1)

Publication Number	Publication Date
ATE49233T1 true ATE49233T1 (de)	1990-01-15

Family

ID=23936984

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT84104456T ATE49233T1 (de)	1983-04-22	1984-04-19	Vektoren zur transformation von hefe.

Country Status (6)

Country	Link
EP (1)	EP0123294B1 (de)
JP (1)	JPS60501140A (de)
AT (1)	ATE49233T1 (de)
DE (1)	DE3480939D1 (de)
IL (1)	IL71615A0 (de)
WO (1)	WO1984004330A1 (de)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
NZ207926A (en) *	1983-04-25	1988-04-29	Genentech Inc	Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
US7198919B1 (en)	1983-04-25	2007-04-03	Genentech, Inc.	Use of alpha factor sequences in yeast expression systems
NZ207842A (en)	1983-04-25	1988-02-12	Chiron Corp	Production of human insulin-like growth factor (igf) using cdna
IL71991A (en)	1983-06-06	1994-05-30	Genentech Inc	Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
US4870008A (en) *	1983-08-12	1989-09-26	Chiron Corporation	Secretory expression in eukaryotes
KR850004274A (ko) *	1983-12-13	1985-07-11	원본미기재	에리트로포이에틴의 제조방법
US4703008A (en) *	1983-12-13	1987-10-27	Kiren-Amgen, Inc.	DNA sequences encoding erythropoietin
NZ210501A (en) *	1983-12-13	1991-08-27	Kirin Amgen Inc	Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
GB2177095B (en) *	1984-07-20	1988-05-25	Celltech Ltd	Eucaryotic expression vectors
US4925791A (en) *	1984-07-20	1990-05-15	Celltech Limited	Eucaryotic expression vectors
US5498600A (en) *	1984-10-12	1996-03-12	Zymogenetics, Inc.	Biologically active mosaic proteins
US5187263A (en) *	1984-10-12	1993-02-16	Zymogenetics, Inc.	Expression of biologically active PDGE analogs in eucaryotic cells
EP0487116B1 (de) *	1984-10-12	1999-12-29	ZymoGenetics, Inc.	Von Blutplättchen abstammende biologisch aktive Analoga eines Wachstumsfaktors in eukaryotischen Zellen
US5045633A (en) *	1985-02-25	1991-09-03	Zymogenetics, Inc.	Expression of biologically active PDGF analogs in eucaryotic cells
FR2593518B1 (fr) *	1985-05-02	1989-09-08	Transgene Sa	Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
US5821079A (en) *	1985-05-02	1998-10-13	Transgene S.A.	Vectors for the expression and secretion of hirudin by transformed yeasts
US4935350A (en) *	1985-11-18	1990-06-19	Amgen	Materials and methods for controlling plasmid copy number and stability
GB8530631D0 (en)	1985-12-12	1986-01-22	Ciba Geigy Ag	Thrombin inhibitors
MY101203A (en) *	1985-12-12	1991-08-17	Ucp Gen Pharma Ag	Production of thrombin iinhibitors.
US5024941A (en) *	1985-12-18	1991-06-18	Biotechnica International, Inc.	Expression and secretion vector for yeast containing a glucoamylase signal sequence
EP0234871A3 (de) *	1986-02-28	1988-10-12	Merck & Co. Inc.	Regulierbare Expression rekombinanter Proteine in Hefe
EP0234862A3 (de) *	1986-02-28	1988-10-05	Merck & Co. Inc.	Expression rekombinanter Proteine in Hefe
FR2599755B1 (fr) *	1986-06-10	1990-04-20	Transgene Sa	Bloc d'expression d'une amyloglucosidase dans une levure, levure transformee et procede de preparation d'enzyme
DK293787A (da) *	1986-06-10	1987-12-11	Transgene Sa	Ekspression af en amyloglucosidase
IT1196484B (it) *	1986-07-11	1988-11-16	Sclavo Spa	Vettore ad espressione e secrezione in lieviti,utile per la preparazione di proteine eterologhe
CA1340772C (en) *	1987-12-30	1999-09-28	Patricia Tekamp-Olson	Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
US5037743A (en) *	1988-08-05	1991-08-06	Zymogenetics, Inc.	BAR1 secretion signal
DK105489D0 (da) *	1989-03-03	1989-03-03	Novo Nordisk As	Polypeptid
FR2686899B1 (fr)	1992-01-31	1995-09-01	Rhone Poulenc Rorer Sa	Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
DE4309333A1 (de) *	1993-03-17	1994-09-22	Silica Gel Gmbh	Superparamagnetische Teilchen, Verfahren zu ihrer Herstellung und Verwendung derselben
US6500645B1 (en)	1994-06-17	2002-12-31	Novo Nordisk A/S	N-terminally extended proteins expressed in yeast
CN102080070B (zh)	1995-03-17	2016-01-20	诺沃奇梅兹有限公司	新的内切葡聚糖酶
GB9526733D0 (en)	1995-12-30	1996-02-28	Delta Biotechnology Ltd	Fusion proteins
DE69740176D1 (de)	1996-03-01	2011-05-26	Novo Nordisk As	Appetithemmendes Peptid, Zusammensetzung und Verwendung
EP2213743A1 (de)	2000-04-12	2010-08-04	Human Genome Sciences, Inc.	Albuminfusionsproteine
US6946134B1 (en)	2000-04-12	2005-09-20	Human Genome Sciences, Inc.	Albumin fusion proteins
CZ20032454A3 (en)	2001-03-22	2004-03-17	Novo Nordisk Health Care Ag	Coagulation factor vii derivative
US7507413B2 (en)	2001-04-12	2009-03-24	Human Genome Sciences, Inc.	Albumin fusion proteins
CZ2004427A3 (cs)	2001-09-27	2004-08-18	Novoánordiskáhealthácareáag	Polypeptidy lidského koagulačního faktoru VII
WO2005003296A2 (en)	2003-01-22	2005-01-13	Human Genome Sciences, Inc.	Albumin fusion proteins
AU2002364586A1 (en)	2001-12-21	2003-07-30	Delta Biotechnology Limited	Albumin fusion proteins
WO2004024885A2 (en)	2002-09-13	2004-03-25	Cornell Research Foundation, Inc.	Using mutations to improve aspergillus phytases
ES2381110T3 (es)	2003-09-09	2012-05-23	Novo Nordisk Health Care Ag	Polipéptidos de factor VII de coagulación
US8673822B2 (en)	2004-01-17	2014-03-18	Korea Research Institute Of Bioscience And Biotechnology	Rapid screening method of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom
CA2604222A1 (en)	2005-04-18	2006-10-26	Novo Nordisk A/S	Il-21 variants
WO2007014197A2 (en) *	2005-07-25	2007-02-01	The G.I. Company, Inc.	Yeast expression vectors for production of itf
JP5690047B2 (ja)	2005-09-14	2015-03-25	ノボノルディスクヘルスケアアーゲー	ヒト凝固第ｖｉｉ因子ポリペプチド
EP2406282A1 (de)	2009-03-11	2012-01-18	Novo Nordisk A/S	Interleukin-21-varianten mit antagonistischer bindung an den il-21-rezeptor
US20120282285A1 (en)	2009-07-17	2012-11-08	Rigshospitalet	Masp isoforms as inhibitors of complement activation
WO2011067283A1 (en)	2009-12-01	2011-06-09	Novo Nordisk A/S	Novel peptidyl alpha-hydroxyglycine alpha-amidating lyases
US9815876B2 (en)	2010-03-05	2017-11-14	Omeros Corporation	Chimeric inhibitor molecules of complement activation
SG10201606195UA (en)	2012-02-09	2016-09-29	Var2 Pharmaceuticals Aps	Targeting of chondroitin sulfate glycans
EP2906693A1 (de)	2012-10-15	2015-08-19	Novo Nordisk Health Care AG	Gerinnungsfaktor-vii-polypeptide
WO2015128507A1 (en) *	2014-02-28	2015-09-03	Novo Nordisk A/S	Mating factor alpha pro-peptide variants
EA037848B1 (ru)	2016-07-14	2021-05-27	Общество С Ограниченной Ответственностью "Биохимический Агент"	Гибридный белок, полинуклеотид, генетическая конструкция, продуцент, препарат для регенерации хряща (варианты)
US20210278406A1 (en)	2018-07-13	2021-09-09	Varct Diagnostic Aps	Isolation of circulating cells of fetal origin using recombinant malaria protein var2csa
WO2022049409A1 (en)	2020-09-02	2022-03-10	Sia Terragen	Express diagnosticum for sars-cov-2

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4411994A (en) *	1978-06-08	1983-10-25	The President And Fellows Of Harvard College	Protein synthesis
US4443539A (en) *	1980-02-05	1984-04-17	The Upjohn Company	Process for preparing bovine growth hormone
US4430428A (en) *	1980-02-05	1984-02-07	The Upjohn Company	Composition of matter and process
US4350764A (en) *	1980-03-10	1982-09-21	The Regents Of The University Of California	Microbiological synthesis of beta endorphin
NZ199722A (en) *	1981-02-25	1985-12-13	Genentech Inc	Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
US4615974A (en) *	1981-08-25	1986-10-07	Celltech Limited	Yeast expression vectors
EP0116201B1 (de) *	1983-01-12	1992-04-22	Chiron Corporation	Sekretorische Expression in Eukaryoten

1984
- 1984-04-18 JP JP59501817A patent/JPS60501140A/ja active Pending
- 1984-04-18 WO PCT/US1984/000601 patent/WO1984004330A1/en not_active Ceased
- 1984-04-19 AT AT84104456T patent/ATE49233T1/de not_active IP Right Cessation
- 1984-04-19 DE DE8484104456T patent/DE3480939D1/de not_active Expired - Fee Related
- 1984-04-19 EP EP84104456A patent/EP0123294B1/de not_active Expired - Lifetime
- 1984-04-22 IL IL71615A patent/IL71615A0/xx unknown

Also Published As

Publication number	Publication date
DE3480939D1 (de)	1990-02-08
EP0123294A1 (de)	1984-10-31
JPS60501140A (ja)	1985-07-25
WO1984004330A1 (en)	1984-11-08
IL71615A0 (en)	1984-07-31
EP0123294B1 (de)	1990-01-03

Legal Events

Date	Code	Title	Description
1990-07-15	UEP	Publication of translation of european patent specification
1998-01-15	REN	Ceased due to non-payment of the annual fee

Publication	Publication Date	Title
ATE49233T1 (de)	1990-01-15	Vektoren zur transformation von hefe.
NZ218332A (en)	1993-04-28	Il-4(interleukin-4), recombinant production, dna, vectors and pharmaceutical composition
ATE80660T1 (de)	1992-10-15	Verfahren zur herstellung eines polypeptides.
IL94183A (en)	2003-09-17	cDNA SEQUENCE CODING FOR HUMAN LACTOFERRIN PROTEIN OR PORTION THEREOF AND LACTOFERRIN PROTEIN PRODUCED FROM SAID SEQUENCE
DE3854952D1 (de)	1996-03-14	Mykobakterielle rekombinanten und peptide
UA27706C2 (uk)	2000-10-16	Спосіб одержання гетерологічного поліпептиду
ES8307297A1 (es)	1983-07-01	Proceso para la produccion microbiana de interferon fibro- blasto humano
NO308310B1 (no)	2000-08-28	Hovedsakelig rent KGF protein og dets anvendelse
ES8506347A1 (es)	1985-07-01	Un metodo para producir un polipeptido de tipo il-2-".
IL94361A0 (en)	1991-03-10	Polypeptides,recombinant dna molecules and method of producing a human hemoglobin like protein
ATE87663T1 (de)	1993-04-15	Verfahren zur herstellung von faktor-viii:c-typ- proteinen.
NZ505500A (en)	2002-12-20	Cell surface glycoproteins associated with human b cell lymphomas - ulbp, dna and polypeptides
DE3586189D1 (de)	1992-07-16	Menge und verfahren zur herstellung rekombinierter erzeugnisse in grosser ausbeute.
NO864759D0 (no)	1986-11-26	Eutaryotiske fusjonsproteiner, fremstilling og anvendelse derav samt middel til gjennomfoering av fremgangsmaaten.
ATE250133T1 (de)	2003-10-15	Verktorvermittelte genominsertion und expression von dna in bcg
NZ238056A (en)	1993-01-27	Surface protein of metastasis forming tumour cells, related polypeptides, dna encoding these, antibodies, assays and pharmaceuticals
ES8606494A1 (es)	1986-04-16	Un metodo de producir un peptido o polipeptido sintetico.
DE69939330D1 (de)	2008-09-25	Wachstumsfaktor-homologe zvegf-3
GR3021636T3 (en)	1997-02-28	Recombinant production of PDGF A-chain polypeptides
DE69013811D1 (de)	1994-12-08	Verfahren zur Herstellung von antiviralem Protein unter Benützung von E.coli-Transformant, das Gen, das für dieses Protein kodiert und E.coli-Vektor benützt in diesem Verfahren.
PT86276A (en)	1988-06-01	Method for the production of hybrid proteins
DE3854879D1 (de)	1996-02-22	Rekombinantes Enzym zur alpha-Amidierung von C-Termini
AU7873798A (en)	1998-07-17	N-terminally extended proteins expressed in yeast
DE69233245D1 (de)	2003-12-11	Verfahren zur Herstellung von Peptiden
ES8802441A1 (es)	1988-06-16	Procedimiento para preparar laminina humana